FastQCFastQC Report
Wed 25 May 2016
SRR1295156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561513
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32260.1259411917878223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70650.020.5998651
CAACGCA77600.018.1787785
ATCAACG77950.018.1567673
TCAACGC77950.018.0971554
TATCAAC79500.017.9768032
AACGCAG78450.017.9579266
CTATACT12650.017.0990684
GAGTACT30850.017.04435212-13
GTCTACG2404.0750743E-516.613931
GTACTAG6100.016.3415721
TACACCG2901.1451084E-616.2146345
GGTATCA54700.016.0368481
GTACATG95600.015.9535451
ACACCGT3252.180459E-715.8941466
TATACTG12500.015.7995385
TACATGG97250.015.6685742
ACGCAGA89800.015.6328397
CGCAGAG91550.015.282738
CATGGGG45400.015.2253274
ACATGGG98550.015.07712653
Produced by FastQC (version 0.11.5)