FastQCFastQC Report
Wed 25 May 2016
SRR1295155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830520
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95100.022.2310451
TACATGG97800.020.913112
CATGGGG40050.019.0116044
AACGCGT2652.2419408E-718.9448768
ACGCGTG2953.3574906E-818.7252569
ACATGGG103200.018.5808723
GTATAAG10400.017.9898951
TCGACTA5600.017.63257694
TAACGCG2302.7613562E-517.3849857
GTATCAA60450.016.4036871
GTATATG16400.016.351791
GTATAAT15550.015.6413751
TACTAAG13050.014.8127182
AGACCGT3051.7958471E-514.8011176
GTATTAT15300.014.6741091
TATCAAC67100.014.5901832
ATCAACG64400.014.5374783
CATGGGT21350.014.5295544
CCTATAC8750.014.1801723
TCAACGC65000.014.1436584
Produced by FastQC (version 0.11.5)