##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295155_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2830520 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.543958000650058 33.0 27.0 33.0 6.0 33.0 2 22.452393553128047 27.0 2.0 33.0 2.0 33.0 3 24.8854927716462 27.0 15.0 33.0 15.0 33.0 4 25.002510846063622 33.0 6.0 37.0 2.0 37.0 5 26.858094625722483 33.0 15.0 37.0 2.0 37.0 6 27.79175169226856 33.0 15.0 37.0 2.0 37.0 7 27.54264976046804 33.0 15.0 37.0 6.0 37.0 8 28.391747452764864 33.0 22.0 37.0 6.0 37.0 9 29.31452100674081 33.0 27.0 37.0 15.0 37.0 10-11 28.809327084775944 33.0 24.5 37.0 10.5 37.0 12-13 27.59609577038848 33.0 15.0 37.0 4.0 37.0 14-15 29.44249554852112 33.0 24.5 37.0 10.5 40.0 16-17 29.861317355114963 35.0 27.0 38.5 6.0 40.0 18-19 30.048454877549002 37.0 27.0 40.0 10.5 40.0 20-21 29.560807554795588 33.0 24.5 40.0 6.0 40.0 22-23 30.308995166965786 35.0 27.0 40.0 15.0 40.0 24-25 30.47709290165765 37.0 27.0 40.0 15.0 40.0 26-27 28.98127075590351 33.0 22.0 38.5 4.0 40.0 28-29 29.70279524610319 33.0 27.0 40.0 10.5 40.0 30-31 30.434111576671427 33.0 27.0 40.0 15.0 40.0 32-33 29.20355747353843 33.0 24.5 38.5 6.0 40.0 34-35 28.8704036714102 33.0 22.0 37.0 6.0 40.0 36-37 29.19951546005681 33.0 22.0 37.0 6.0 40.0 38-39 29.293600822463716 33.0 24.5 37.0 6.0 40.0 40-41 28.391052704096772 33.0 22.0 37.0 6.0 40.0 42-43 28.985656522476436 33.0 22.0 37.0 6.0 40.0 44-45 28.889116840721847 33.0 22.0 37.0 6.0 40.0 46-47 28.561759676667187 33.0 22.0 37.0 6.0 40.0 48-49 28.508288053078587 33.0 22.0 37.0 6.0 40.0 50-51 28.435009291578933 33.0 22.0 37.0 6.0 40.0 52-53 28.680321637013694 33.0 22.0 37.0 6.0 40.0 54-55 28.666556145160605 33.0 22.0 37.0 6.0 40.0 56-57 28.68265795684185 33.0 22.0 37.0 6.0 40.0 58-59 28.500924388451594 33.0 22.0 37.0 6.0 40.0 60-61 28.470982187018638 33.0 22.0 37.0 10.5 40.0 62-63 28.457044112035952 33.0 22.0 37.0 10.5 40.0 64-65 28.018557367550837 33.0 22.0 37.0 6.0 40.0 66-67 28.207481840792504 33.0 22.0 37.0 10.5 40.0 68-69 27.761143712109437 33.0 22.0 37.0 6.0 38.5 70-71 27.913929066037333 33.0 22.0 37.0 10.5 37.0 72-73 27.95496975820697 33.0 22.0 37.0 10.5 37.0 74-75 28.06839873945423 33.0 22.0 37.0 15.0 37.0 76-77 27.82414097056371 33.0 22.0 37.0 15.0 37.0 78-79 27.375724071901985 33.0 22.0 37.0 10.5 37.0 80-81 27.19783626330144 27.0 22.0 33.0 10.5 37.0 82-83 27.28782838489041 27.0 22.0 33.0 15.0 37.0 84-85 27.246741588118084 27.0 22.0 33.0 10.5 37.0 86-87 27.087571894916834 27.0 22.0 33.0 15.0 37.0 88-89 27.006821184799968 27.0 22.0 33.0 15.0 37.0 90-91 27.159474054237386 27.0 22.0 33.0 15.0 37.0 92-93 27.065156225711178 27.0 22.0 33.0 15.0 37.0 94-95 26.934533230643133 27.0 22.0 33.0 15.0 37.0 96-97 27.022551863261874 27.0 22.0 33.0 15.0 37.0 98-99 27.106629877195708 27.0 22.0 33.0 15.0 37.0 100 26.76568793013298 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.373888914135808 1101 2 11.290694981930638 1101 3 8.500167967494956 1101 4 12.894006161609202 1101 5 10.884988979702662 1101 6 10.036182106451925 1101 7 9.468828700161982 1101 8 9.020751268500362 1101 9 7.338776495088975 1101 10-11 7.774511708682773 1101 12-13 9.154347153522014 1101 14-15 8.036312909631828 1101 16-17 8.290392167942944 1101 18-19 8.22846225999864 1101 20-21 7.4478060858822595 1101 22-23 7.459043875966433 1101 24-25 7.47232077806575 1101 26-27 8.628602233558471 1101 28-29 8.273072974264046 1101 30-31 5.517757713756303 1101 32-33 7.17333660059224 1101 34-35 8.836636717271194 1101 36-37 8.223261077973142 1101 38-39 8.299768757533588 1101 40-41 9.141704389461811 1101 42-43 8.729970886944777 1101 44-45 8.663343464927102 1101 46-47 7.541842769498729 1101 48-49 5.631819298389551 1101 50-51 6.839449997052672 1101 52-53 6.05342965484143 1101 54-55 4.272879909435851 1101 56-57 6.286579859903355 1101 58-59 6.3354047475022846 1101 60-61 6.889558460807791 1101 62-63 6.366533009798875 1101 64-65 6.687824605960245 1101 66-67 5.951213707312942 1101 68-69 6.877264168118398 1101 70-71 6.39549667027233 1101 72-73 5.677694548267631 1101 74-75 5.054198001399623 1101 76-77 5.217714557701665 1101 78-79 5.2913912109902945 1101 80-81 5.053444293126873 1101 82-83 4.682169512038648 1101 84-85 4.421612382087758 1101 86-87 4.137507932189223 1101 88-89 4.1003055667284976 1101 90-91 3.947973504888555 1101 92-93 3.941041708682203 1101 94-95 3.7972012284332948 1101 96-97 3.9400649887501906 1101 98-99 3.557318145525638 1101 100 3.804884699921601 1102 1 6.709243486328557 1102 2 10.457138072072151 1102 3 8.184287660875722 1102 4 12.593133251698266 1102 5 10.619614424379222 1102 6 9.392264451372341 1102 7 6.45145818079725 1102 8 7.701453780977523 1102 9 6.268938403243052 1102 10-11 7.242752024129977 1102 12-13 8.96514754946988 1102 14-15 5.9588371428652245 1102 16-17 7.221031666365274 1102 18-19 7.397128842173121 1102 20-21 6.9153023602138255 1102 22-23 6.388493127118007 1102 24-25 6.873829892296271 1102 26-27 8.45921684866742 1102 28-29 7.6315385997830205 1102 30-31 4.1964764989917285 1102 32-33 4.377265487305696 1102 34-35 4.993073492350074 1102 36-37 5.151463459341741 1102 38-39 4.378436820142301 1102 40-41 6.0675288165173775 1102 42-43 6.94988649673072 1102 44-45 6.441094095340802 1102 46-47 5.5057199284197935 1102 48-49 3.622733857121819 1102 50-51 4.870570964371673 1102 52-53 5.792299056505634 1102 54-55 5.31725551581297 1102 56-57 6.195665601628221 1102 58-59 6.034317147313484 1102 60-61 6.062071276670181 1102 62-63 4.387181844243717 1102 64-65 6.056033127580051 1102 66-67 3.6956886830578597 1102 68-69 6.006169427975575 1102 70-71 5.325224691013702 1102 72-73 5.02610332729725 1102 74-75 4.120597743771292 1102 76-77 3.181095579955162 1102 78-79 4.260584576459795 1102 80-81 4.475200530616931 1102 82-83 3.8592518165339236 1102 84-85 3.0695859353047297 1102 86-87 3.150072748986851 1102 88-89 3.3940599370140525 1102 90-91 3.296707597049135 1102 92-93 3.206206872699017 1102 94-95 3.059898410254654 1102 96-97 3.277603201434509 1102 98-99 3.050804453420998 1102 100 3.252849512567458 1103 1 6.818323072637689 1103 2 11.041007868898117 1103 3 7.984500048049362 1103 4 12.034199482414746 1103 5 10.381532559029637 1103 6 9.283351948514884 1103 7 8.077071270386401 1103 8 8.087611346491279 1103 9 7.063911214082697 1103 10-11 7.366202172717877 1103 12-13 8.7948517591139 1103 14-15 8.64808769184381 1103 16-17 8.17873807885081 1103 18-19 7.390802530685793 1103 20-21 8.471171616720174 1103 22-23 7.002323092689952 1103 24-25 5.477324444073439 1103 26-27 6.089256195019228 1103 28-29 7.21655054915923 1103 30-31 6.259873516150343 1103 32-33 8.030210229233806 1103 34-35 7.222793980854423 1103 36-37 4.130033551958068 1103 38-39 6.548650177120383 1103 40-41 7.000145833814532 1103 42-43 7.601255999151775 1103 44-45 5.739293653387058 1103 46-47 6.431196968299535 1103 48-49 5.822217644069639 1103 50-51 6.558613414097703 1103 52-53 6.311409399470044 1103 54-55 5.136476208668977 1103 56-57 6.011280700098013 1103 58-59 5.6457097864977435 1103 60-61 5.199721856128338 1103 62-63 2.944149127198017 1103 64-65 5.133877945014362 1103 66-67 4.236591224638225 1103 68-69 5.346843649880686 1103 70-71 3.94893135287753 1103 72-73 4.575642670185466 1103 74-75 3.303348940440703 1103 76-77 3.66002290696629 1103 78-79 3.0001474024699526 1103 80-81 4.2403197819279015 1103 82-83 3.632009524747474 1103 84-85 3.41911045403927 1103 86-87 2.87654460041281 1103 88-89 3.1418581345632184 1103 90-91 2.924821743032414 1103 92-93 2.515770668513621 1103 94-95 2.9851318214032503 1103 96-97 3.0865941891803352 1103 98-99 2.7959352731135674 1103 100 3.0993502503115558 1104 1 6.533469536838272 1104 2 9.91645096945431 1104 3 7.669359234065077 1104 4 12.394622542360008 1104 5 9.806804563973579 1104 6 8.245530106897505 1104 7 4.096921186515999 1104 8 6.876632269948502 1104 9 6.722230627872758 1104 10-11 7.311831553842623 1104 12-13 7.638697437581275 1104 14-15 7.563265232234027 1104 16-17 7.556164405327376 1104 18-19 7.184135981731959 1104 20-21 7.763993370052919 1104 22-23 6.6959584771695155 1104 24-25 6.292048554605799 1104 26-27 8.570980923543996 1104 28-29 7.416607757599245 1104 30-31 6.569297370657274 1104 32-33 7.9600419587297 1104 34-35 8.228282812848779 1104 36-37 6.88507195577197 1104 38-39 7.528058316963243 1104 40-41 7.421136034220641 1104 42-43 7.558724702250565 1104 44-45 7.028011937133861 1104 46-47 7.0158845289952225 1104 48-49 5.808120707546287 1104 50-51 5.978741603379948 1104 52-53 6.275175268632225 1104 54-55 5.04232524195228 1104 56-57 6.189784452194772 1104 58-59 4.208560673758274 1104 60-61 4.634048685827288 1104 62-63 4.143735248854238 1104 64-65 3.204900104456403 1104 66-67 3.6658875961399495 1104 68-69 4.4809917914892345 1104 70-71 2.907056893620883 1104 72-73 2.7654002405924594 1104 74-75 3.416725474386144 1104 76-77 4.188345322433758 1104 78-79 4.616002968815852 1104 80-81 3.4075312236159014 1104 82-83 3.253430554146256 1104 84-85 3.223984471872768 1104 86-87 0.8617484496226595 1104 88-89 0.564378837308869 1104 90-91 2.513803821252594 1104 92-93 2.7003879546441034 1104 94-95 2.6965131227186916 1104 96-97 3.206601958227747 1104 98-99 2.7622207856058445 1104 100 1.1802698230139477 1105 1 5.812711537354197 1105 2 -2.7344282016736905 1105 3 0.4659241646095893 1105 4 8.69146122399556 1105 5 8.768546778280239 1105 6 8.051015334134092 1105 7 5.142662227623898 1105 8 5.963733797580517 1105 9 4.9930043948655864 1105 10-11 4.974635202066747 1105 12-13 5.081983878301166 1105 14-15 5.946450834399503 1105 16-17 6.10162632407275 1105 18-19 6.1565560999706115 1105 20-21 7.150275100309077 1105 22-23 2.163258077504512 1105 24-25 4.1640365257846845 1105 26-27 7.133459850137793 1105 28-29 6.0882975949415865 1105 30-31 5.405454494482747 1105 32-33 7.20252827788924 1105 34-35 6.189566928373516 1105 36-37 6.241104140996022 1105 38-39 4.132711619058394 1105 40-41 5.434563449614281 1105 42-43 5.66072731014421 1105 44-45 6.203507659552201 1105 46-47 5.612227979401069 1105 48-49 5.129911517724846 1105 50-51 5.922058038410537 1105 52-53 5.706012176334696 1105 54-55 5.462577578632057 1105 56-57 5.177754528558701 1105 58-59 1.9474846808681399 1105 60-61 4.481686845884113 1105 62-63 -0.8824242284362391 1105 64-65 -0.5854432964772087 1105 66-67 0.8453865130513236 1105 68-69 2.930329761077939 1105 70-71 3.0780958883450253 1105 72-73 2.807101788720111 1105 74-75 2.758991246312064 1105 76-77 1.9143655707137128 1105 78-79 3.2896392983998943 1105 80-81 1.643035243235179 1105 82-83 1.0881213491297714 1105 84-85 1.448141666372866 1105 86-87 -0.16784416063858743 1105 88-89 1.601087760333602 1105 90-91 1.4269731894567883 1105 92-93 1.8581241050679758 1105 94-95 1.2252884516207274 1105 96-97 2.1112060283793816 1105 98-99 2.0055191844722238 1105 100 2.1232705403613856 1106 1 6.315317124649781 1106 2 10.455217655743663 1106 3 6.916698398138749 1106 4 11.812669160250291 1106 5 10.070803274482927 1106 6 9.426189625300779 1106 7 6.617363257550117 1106 8 7.584759565101699 1106 9 6.963067507305379 1106 10-11 5.7341765766890305 1106 12-13 8.000625926914939 1106 14-15 7.933047728800059 1106 16-17 7.802013941391063 1106 18-19 7.111619176304888 1106 20-21 8.20610419616791 1106 22-23 6.2460439199413536 1106 24-25 5.083278336233356 1106 26-27 7.764319611066764 1106 28-29 6.403614891846431 1106 30-31 5.514339741638125 1106 32-33 7.456356159946306 1106 34-35 6.803196809428822 1106 36-37 6.017857465821585 1106 38-39 7.263023724974904 1106 40-41 6.749403169028479 1106 42-43 6.373567104067064 1106 44-45 4.719961927322515 1106 46-47 6.233262896387927 1106 48-49 5.965750819956234 1106 50-51 6.710288978145176 1106 52-53 5.798853568747155 1106 54-55 5.429339005863362 1106 56-57 4.955041845741711 1106 58-59 5.012521708714683 1106 60-61 4.907543555897089 1106 62-63 5.113224974977637 1106 64-65 5.2989619439428495 1106 66-67 3.1808166741541335 1106 68-69 3.5394702906342452 1106 70-71 3.02352026928315 1106 72-73 2.9176872363595656 1106 74-75 2.866658014402404 1106 76-77 3.6558369968093345 1106 78-79 4.279855770654777 1106 80-81 3.7642517545496617 1106 82-83 2.2535626848893955 1106 84-85 2.9009551639928546 1106 86-87 3.5586692020124246 1106 88-89 3.5832689502794643 1106 90-91 3.2843477900805595 1106 92-93 3.097186513778464 1106 94-95 1.6963799968251578 1106 96-97 1.633661442992132 1106 98-99 2.1731782604467575 1106 100 3.0383270651858965 1107 1 6.068611362292412 1107 2 10.36031106056036 1107 3 6.238113608822037 1107 4 11.432477328205241 1107 5 9.576257622137014 1107 6 7.881245706030889 1107 7 3.668574011359066 1107 8 6.202953724312149 1107 9 5.444409979019916 1107 10-11 4.363686964653134 1107 12-13 5.971899065268627 1107 14-15 6.0848622685097915 1107 16-17 6.956983720920949 1107 18-19 6.160363121890843 1107 20-21 5.535329837471394 1107 22-23 5.705002474725209 1107 24-25 4.594270236734566 1107 26-27 7.11836555495336 1107 28-29 6.3336865171126036 1107 30-31 5.025297134559274 1107 32-33 4.9953180128378065 1107 34-35 6.664218121998264 1107 36-37 6.380324441744968 1107 38-39 6.392857258688704 1107 40-41 7.740059677351578 1107 42-43 6.136405858852974 1107 44-45 3.916702648760989 1107 46-47 3.4545024946637923 1107 48-49 3.931761713601503 1107 50-51 4.734000630656215 1107 52-53 3.882576912985318 1107 54-55 5.278753301504526 1107 56-57 5.16055824253975 1107 58-59 3.0622354140504804 1107 60-61 2.8318916528846714 1107 62-63 0.49609152072801876 1107 64-65 2.0820428881906423 1107 66-67 4.085081252047953 1107 68-69 4.957850618776607 1107 70-71 4.130982356095192 1107 72-73 2.3146732948672195 1107 74-75 2.594961239065885 1107 76-77 3.155946969564475 1107 78-79 3.8061949442616587 1107 80-81 2.1037854872679205 1107 82-83 1.5137132884387938 1107 84-85 2.3561378800167603 1107 86-87 2.3502826560556365 1107 88-89 2.587508330114826 1107 90-91 1.3306128874155903 1107 92-93 2.148687840761543 1107 94-95 2.2263909489505664 1107 96-97 2.1977498666772775 1107 98-99 1.7433003506300118 1107 100 -0.13006713048863716 1108 1 4.278529154594764 1108 2 -3.355597266199533 1108 3 -0.08485138808553216 1108 4 6.501878517706231 1108 5 6.828338663901086 1108 6 6.7196102591627636 1108 7 4.067581128591989 1108 8 5.088322201271101 1108 9 4.758737349600445 1108 10-11 3.1970981314130427 1108 12-13 2.406260678165186 1108 14-15 3.962286268338623 1108 16-17 4.459002049750897 1108 18-19 3.924664292693894 1108 20-21 4.519363585567348 1108 22-23 4.009014384491092 1108 24-25 2.8792904302993563 1108 26-27 4.664694800914116 1108 28-29 6.003788328662658 1108 30-31 4.362843165325927 1108 32-33 4.516463615301465 1108 34-35 2.7658302291149077 1108 36-37 1.104237863746306 1108 38-39 2.4639375079510053 1108 40-41 5.381157376092556 1108 42-43 3.004578449274483 1108 44-45 3.606665475274035 1108 46-47 4.634170624140388 1108 48-49 4.707290765710788 1108 50-51 2.10227505054576 1108 52-53 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12-13 3.8426752518546294 1109 14-15 1.758280247557284 1109 16-17 2.070641831020584 1109 18-19 2.565879856095936 1109 20-21 3.240056803928308 1109 22-23 3.567911011738481 1109 24-25 3.6780158258933895 1109 26-27 5.445226036965401 1109 28-29 4.793086590979009 1109 30-31 4.043557983294193 1109 32-33 5.821193197053969 1109 34-35 1.088348842146253 1109 36-37 2.8043133446893656 1109 38-39 2.625950365000545 1109 40-41 3.963205392110286 1109 42-43 0.9539638183407142 1109 44-45 2.763896917411582 1109 46-47 3.4110938343898844 1109 48-49 1.9911349310458561 1109 50-51 3.8541129980160775 1109 52-53 2.9343906886653635 1109 54-55 3.481727459058604 1109 56-57 4.269848135347985 1109 58-59 2.335559339388727 1109 60-61 0.6204737485133265 1109 62-63 1.5673660244015473 1109 64-65 2.92801405375036 1109 66-67 2.494881686789096 1109 68-69 1.4963975131485405 1109 70-71 0.21689855821023585 1109 72-73 0.4082793408461356 1109 74-75 0.9403593439111972 1109 76-77 0.023340292887006342 1109 78-79 1.5526019980986625 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46-47 -17.522541659315312 2213 48-49 -6.280382766662054 2213 50-51 -24.016407332790262 2213 52-53 -18.339442512187894 2213 54-55 -18.226013308163527 2213 56-57 -18.111572111292755 2213 58-59 -18.601776319762163 2213 60-61 -19.988766501292353 2213 62-63 -18.082948996712748 2213 64-65 -18.241031761676396 2213 66-67 -8.887036271343153 2213 68-69 -23.320625229787364 2213 70-71 -17.954555514132085 2213 72-73 -9.824834992849958 2213 74-75 -4.631617164136777 2213 76-77 -3.4620791029856335 2213 78-79 -4.902306381071153 2213 80-81 -3.5097660767226095 2213 82-83 -3.4585203849683595 2213 84-85 -2.9593308137062238 2213 86-87 -3.225298388894636 2213 88-89 -3.0382826407065657 2213 90-91 -3.0473362441994283 2213 92-93 -3.8118498856267067 2213 94-95 -3.8967089734338956 2213 96-97 -3.570557461518508 2213 98-99 -3.9140507371382647 2213 100 -3.1731934516501177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 750.0 3 786.0 4 4963.0 5 17254.0 6 20856.0 7 24244.0 8 21572.0 9 21822.0 10 25946.0 11 32586.0 12 38651.0 13 51999.0 14 39995.0 15 44170.0 16 51574.0 17 54682.0 18 54759.0 19 55750.0 20 55675.0 21 54178.0 22 56997.0 23 62637.0 24 69191.0 25 76953.0 26 83761.0 27 90835.0 28 97625.0 29 102998.0 30 108022.0 31 117325.0 32 133930.0 33 162683.0 34 199540.0 35 235541.0 36 260694.0 37 227794.0 38 71659.0 39 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.32048537034179 21.088099712837558 14.012072292115137 23.579342624705514 2 14.940613041308062 21.18035598142574 40.711263291637046 23.16776768562915 3 17.518701923367434 26.960491809222255 30.560914188687 24.959892078723314 4 12.025923574191705 17.950153821641873 39.57604740471376 30.44787519945266 5 12.70330040153036 38.61255592221716 35.77184751519812 12.91229616105436 6 30.087003105907193 38.567567227887494 18.752916206488628 12.592513459716681 7 26.295120902835578 32.54019287655123 23.909811875903593 17.254874344709595 8 23.700452260367417 37.049102331694975 21.907957851327193 17.342487556610415 9 24.404972707429692 17.804339121241558 22.29721081014917 35.49347736117958 10-11 22.96505820148063 27.8724785457186 29.406199477056344 19.756263775744426 12-13 23.698517701438398 25.614479197839046 29.235087988036124 21.451915112686432 14-15 21.16326484493109 27.276278943636967 26.663728877699498 24.896727333732443 16-17 20.76972401660897 30.774841847095715 27.42764202461143 21.02779211168389 18-19 20.828552443096505 29.71604260175952 29.02967923590666 20.425725719237317 20-21 22.3343906500891 28.977876026996125 28.111493445643326 20.576239877271448 22-23 21.51265682773441 28.85239043315079 28.479555397041096 21.1553973420737 24-25 21.44752656472269 28.84861593907627 28.155479527612997 21.548377968588046 26-27 21.38049855306156 28.80216487657818 28.27761770670001 21.53971886366025 28-29 21.418677212417744 28.587297189872185 28.617481650252998 21.376543947457073 30-31 21.81005554355449 28.950265289273318 28.13372029366768 21.10595887350451 32-33 21.21531369604799 28.710815363520737 28.558752747899725 21.515118192531553 34-35 21.212712430828276 28.41893695227542 28.428653955048766 21.93969666184754 36-37 21.69037963514832 28.98075485675292 28.040460781573913 21.288404726524846 38-39 21.426954563724422 28.530312467020476 28.342917966103233 21.699815003151876 40-41 21.608690962192682 28.595352638764147 28.09045346588016 21.70550293316301 42-43 21.448152337822492 28.732786634527535 28.38165416218386 21.43740686546612 44-45 21.70197239335108 28.426141387220756 28.32810237584046 21.543783843587708 46-47 21.593814578413635 28.65679539823583 28.51109499342029 21.238295029930253 48-49 21.448009140018115 28.464569891045556 28.477883103357733 21.6095378655786 50-51 21.650636935472928 28.58213608417951 28.1417706180909 21.62545636225666 52-53 21.34060388106304 28.437354050713886 28.349152283010405 21.87288978521267 54-55 21.686298035088726 28.541719674742755 28.028077233984455 21.74390505618406 56-57 21.807462916808383 28.591870226950434 27.974600731906317 21.626066124334862 58-59 21.526876671212783 28.213144629045043 28.34787207185856 21.912106627883613 60-61 21.80495710953868 28.508773712224013 28.086814623580274 21.59945455465703 62-63 21.488767935294444 28.592070274572922 28.405391595158747 21.513770194973887 64-65 21.40270117317997 28.224967148294155 28.300448294303877 22.071883384221998 66-67 21.595226595099486 28.355583075185486 28.137140968699043 21.912049361015985 68-69 22.057179060032837 28.32787288196217 27.99720132022078 21.617746737784213 70-71 21.95647101230183 28.330850087299492 27.984666523210404 21.728012377188275 72-73 21.676315690644486 28.450374356015473 28.260496514294204 21.612813439045837 74-75 21.944509987446633 28.134385173768955 27.892994851052638 22.028109987731774 76-77 21.749651007126953 28.492223735523798 28.057452252710746 21.700673004638503 78-79 22.02456489394706 28.286230285353557 28.108365682712872 21.58083913798651 80-81 21.988579121046467 28.136997798452253 27.99538843760201 21.879034642899274 82-83 21.49942725005085 28.341094898064785 28.388877588526444 21.77060026335792 84-85 21.66047183064755 28.065941836190987 28.105671017992158 22.167915315169303 86-87 21.889440076686174 28.185646104795687 28.08129174484818 21.84362207366996 88-89 21.861569003734164 27.918676941430714 28.262766698033836 21.95698735680129 90-91 21.981612878073676 27.952182141667315 28.01803270554378 22.048172274715228 92-93 21.68617370349363 28.123682141778083 28.265400966874378 21.92474318785391 94-95 21.64507706609028 27.828785328033195 28.250122154962902 22.27601545091362 96-97 21.796770522122635 27.96866552852468 28.19040759709774 22.044156352254944 98-99 21.7748360170352 27.829389553088728 28.224826715555057 22.170947714321013 100 21.851203790471775 27.741394437437165 27.996144969619063 22.411256802471996 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 329.0 1 369.0 2 355.0 3 318.5 4 373.0 5 529.0 6 821.0 7 1284.5 8 1953.5 9 2787.5 10 3689.0 11 4693.0 12 5679.0 13 6602.5 14 7436.5 15 8165.0 16 8886.0 17 9595.0 18 10322.5 19 11130.0 20 12149.0 21 13133.0 22 14287.5 23 15985.0 24 18133.5 25 21167.0 26 25364.5 27 30815.5 28 37720.5 29 45807.0 30 54500.0 31 63206.0 32 71582.0 33 79822.5 34 87243.5 35 93690.0 36 99079.0 37 103253.5 38 107054.5 39 108844.5 40 108499.5 41 107406.5 42 105057.5 43 101997.5 44 98424.0 45 95363.5 46 92311.5 47 88257.5 48 84407.5 49 81303.0 50 79407.0 51 75391.0 52 68049.5 53 61073.0 54 55443.5 55 50516.0 56 45879.0 57 41570.5 58 37615.0 59 33482.0 60 28874.5 61 23589.5 62 18230.0 63 13923.5 64 10878.0 65 8541.5 66 6657.5 67 5142.0 68 3924.5 69 2952.0 70 2217.0 71 1706.0 72 1273.5 73 931.0 74 677.0 75 470.5 76 319.5 77 230.0 78 168.5 79 109.5 80 77.5 81 51.5 82 35.0 83 24.5 84 16.5 85 12.0 86 9.5 87 8.0 88 5.0 89 4.5 90 4.0 91 2.5 92 1.5 93 0.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.208350409112107 2 25.256984582338227 3 6.453372525189718 4 24.6087291381089 5 13.078268304057206 6 10.047765074968556 7 8.191533711120218 8 9.25935870440767 9 5.329515424727612 10-11 7.0735235928380655 12-13 10.5126266551729 14-15 7.76228396195752 16-17 8.29153300453627 18-19 6.797796871246272 20-21 8.217271031471249 22-23 6.094569195766149 24-25 5.363908398456821 26-27 10.257090569930613 28-29 6.7156741517459695 30-31 4.488786512725577 32-33 9.239026751268318 34-35 7.286788293317128 36-37 7.145206534488363 38-39 6.433323205630061 40-41 8.622549920156015 42-43 6.7902540875881465 44-45 6.757221287961222 46-47 6.33060356400944 48-49 4.201630795754844 50-51 7.049005129799471 52-53 4.649128075406638 54-55 5.095017876573916 56-57 4.95615646594972 58-59 3.9986292271384762 60-61 4.371670223139211 62-63 3.065443805378517 64-65 4.610742902364231 66-67 2.7178751607478486 68-69 5.42456863049899 70-71 2.7227329253988666 72-73 1.6937347201220976 74-75 0.8796263584076425 76-77 1.286283085793423 78-79 1.8371889264163475 80-81 2.214946370278253 82-83 0.9980498283001004 84-85 1.471037123920693 86-87 0.4154360329550754 88-89 0.734423356839026 90-91 0.31725619320831505 92-93 0.4332596130746294 94-95 0.6542260786003985 96-97 0.6684284159801026 98-99 0.48627107386628604 100 0.6208046578013934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2830520.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.14393113314043 #Duplication Level Percentage of deduplicated Percentage of total 1 96.60146386717497 90.94441561671873 2 2.667487566117027 5.022555314460595 3 0.41129047171456784 1.1616150553443936 4 0.12972131692849118 0.4884989890966463 5 0.06094754532884253 0.2868920755086253 6 0.030826595524868394 0.1741282131697431 7 0.01870729278046378 0.12328246582480341 8 0.016954985870820498 0.1276967217748715 9 0.009416322826238862 0.07978406829127647 >10 0.04762129351160085 0.787584339904007 >50 0.002939880140780771 0.1934492071966383 >100 0.002433942364530292 0.45507825265571333 >500 1.1335183027842531E-4 0.06203632917337667 >1k 7.556788657672184E-5 0.0929833508805092 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.532919746195046E-5 0.0 0.0 0.0 0.0 3 3.532919746195046E-5 0.0 0.0 0.0 0.0 4 3.532919746195046E-5 0.0 0.0 0.0 0.0 5 3.532919746195046E-5 0.0 0.0 3.532919746195046E-5 0.0 6 3.532919746195046E-5 0.0 0.0 3.532919746195046E-5 0.0 7 3.532919746195046E-5 0.0 3.532919746195046E-5 3.532919746195046E-5 0.0 8 3.532919746195046E-5 0.0 3.532919746195046E-5 3.532919746195046E-5 0.0 9 7.065839492390091E-5 0.0 3.532919746195046E-5 3.532919746195046E-5 0.0 10-11 7.065839492390091E-5 0.0 3.532919746195046E-5 7.065839492390091E-5 0.0 12-13 7.065839492390091E-5 0.0 3.532919746195046E-5 7.065839492390091E-5 0.0 14-15 7.065839492390091E-5 0.0 3.532919746195046E-5 7.065839492390091E-5 0.0 16-17 7.065839492390091E-5 0.0 3.532919746195046E-5 7.065839492390091E-5 0.0 18-19 7.065839492390091E-5 0.0 3.532919746195046E-5 7.065839492390091E-5 0.0 20-21 7.065839492390091E-5 0.0 3.532919746195046E-5 1.236521911168266E-4 0.0 22-23 7.065839492390091E-5 0.0 3.532919746195046E-5 1.7664598730975228E-4 0.0 24-25 7.065839492390091E-5 0.0 3.532919746195046E-5 2.1197518477170273E-4 0.0 26-27 7.065839492390091E-5 0.0 3.532919746195046E-5 2.8263357969560366E-4 0.0 28-29 7.065839492390091E-5 0.0 3.532919746195046E-5 5.47602560660232E-4 0.0 30-31 7.065839492390091E-5 0.0 3.532919746195046E-5 0.002561366815991408 0.0 32-33 7.065839492390091E-5 0.0 3.532919746195046E-5 0.0077017650467052 0.0 34-35 7.065839492390091E-5 0.0 3.532919746195046E-5 0.01524454870483162 0.0 36-37 7.065839492390091E-5 0.0 3.532919746195046E-5 0.026443904300269917 0.0 38-39 7.065839492390091E-5 3.532919746195046E-5 3.532919746195046E-5 0.04416149682743807 0.0 40-41 7.065839492390091E-5 3.532919746195046E-5 3.532919746195046E-5 0.06452877916425251 0.0 42-43 7.065839492390091E-5 3.532919746195046E-5 3.532919746195046E-5 0.08618557720842814 0.0 44-45 7.065839492390091E-5 3.532919746195046E-5 3.532919746195046E-5 0.11257648771250511 0.0 46-47 7.065839492390091E-5 3.532919746195046E-5 3.532919746195046E-5 0.14555629354323585 0.0 48-49 7.065839492390091E-5 7.065839492390091E-5 3.532919746195046E-5 0.1810444723937651 0.0 50-51 7.065839492390091E-5 7.065839492390091E-5 3.532919746195046E-5 0.21909401806028575 0.0 52-53 7.065839492390091E-5 7.065839492390091E-5 3.532919746195046E-5 0.25857439622401535 0.0 54-55 7.065839492390091E-5 7.065839492390091E-5 3.532919746195046E-5 0.30535025366363777 0.0 56-57 7.065839492390091E-5 7.065839492390091E-5 3.532919746195046E-5 0.3611880502522504 0.0 58-59 7.065839492390091E-5 7.065839492390091E-5 3.532919746195046E-5 0.4226961830335062 0.0 60-61 7.065839492390091E-5 8.832299365487614E-5 3.532919746195046E-5 0.4896803414213643 0.0 62-63 7.065839492390091E-5 1.0598759238585136E-4 3.532919746195046E-5 0.5586959286632844 0.0 64-65 7.065839492390091E-5 1.0598759238585136E-4 3.532919746195046E-5 0.6369677656402357 0.0 66-67 7.065839492390091E-5 1.4131678984780183E-4 3.532919746195046E-5 0.7315440272458771 0.0 68-69 7.065839492390091E-5 1.4131678984780183E-4 3.532919746195046E-5 0.8341400166753812 0.0 70-71 7.065839492390091E-5 1.943105860407275E-4 3.532919746195046E-5 0.954842219804135 0.0 72-73 7.065839492390091E-5 2.1197518477170273E-4 3.532919746195046E-5 1.0848713310628435 0.0 74-75 7.065839492390091E-5 2.1197518477170273E-4 3.532919746195046E-5 1.2218956234190184 0.0 76-77 7.065839492390091E-5 2.1197518477170273E-4 3.532919746195046E-5 1.375489309384848 0.0 78-79 7.065839492390091E-5 2.1197518477170273E-4 7.065839492390091E-5 1.549538600681147 0.0 80-81 7.065839492390091E-5 2.473043822336532E-4 7.065839492390091E-5 1.745509659002586 0.0 82-83 7.065839492390091E-5 2.473043822336532E-4 7.065839492390091E-5 1.9655575653943442 0.0 84-85 1.0598759238585136E-4 2.473043822336532E-4 7.065839492390091E-5 2.190427907239659 0.0 86-87 1.0598759238585136E-4 2.8263357969560366E-4 7.065839492390091E-5 2.4328391956248323 0.0 88 1.4131678984780183E-4 2.8263357969560366E-4 7.065839492390091E-5 2.641140143860492 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9510 0.0 22.231045 1 TACATGG 9780 0.0 20.91311 2 CATGGGG 4005 0.0 19.011604 4 AACGCGT 265 2.2419408E-7 18.944876 8 ACGCGTG 295 3.3574906E-8 18.725256 9 ACATGGG 10320 0.0 18.580872 3 GTATAAG 1040 0.0 17.989895 1 TCGACTA 560 0.0 17.632576 94 TAACGCG 230 2.7613562E-5 17.384985 7 GTATCAA 6045 0.0 16.403687 1 GTATATG 1640 0.0 16.35179 1 GTATAAT 1555 0.0 15.641375 1 TACTAAG 1305 0.0 14.812718 2 AGACCGT 305 1.7958471E-5 14.801117 6 GTATTAT 1530 0.0 14.674109 1 TATCAAC 6710 0.0 14.590183 2 ATCAACG 6440 0.0 14.537478 3 CATGGGT 2135 0.0 14.529554 4 CCTATAC 875 0.0 14.180172 3 TCAACGC 6500 0.0 14.143658 4 >>END_MODULE