FastQCFastQC Report
Wed 25 May 2016
SRR1295155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830520
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40590.1434012124980569No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30500.10775405225894889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC852.501274E-741.0383341
GTATCAA93350.024.5558031
TCGCGGT1603.4501696E-521.8016151
TCAACGC105350.021.2015194
ATCAACG105200.021.185553
CAACGCA105600.021.062275
AACGCAG108000.020.8285396
TATCAAC108950.020.4744012
TACGACT2001.4027726E-518.8090934
GAGTACT48200.018.1171612-13
CGTTACG650.00810126618.0541918-19
GGTATCA71750.017.2242531
GTATAGG9000.017.1644461
ACGCAGA136850.016.158767
CGCAGAG138000.015.9900828
GTACATG119000.015.95471
AGTACTT54450.015.77892612-13
GCAGAGT137250.015.6324879
GTACTTT57800.015.43271514-15
CGTACCC2155.17532E-415.3086453
Produced by FastQC (version 0.11.5)