##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295155_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2830520 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38713769908003 33.0 33.0 33.0 27.0 33.0 2 30.005345307575993 33.0 33.0 33.0 27.0 33.0 3 30.84287551403982 33.0 33.0 33.0 27.0 33.0 4 35.14600285459915 37.0 37.0 37.0 33.0 37.0 5 35.44559939516414 37.0 37.0 37.0 33.0 37.0 6 35.201269024772834 37.0 37.0 37.0 33.0 37.0 7 35.099844904823144 37.0 37.0 37.0 33.0 37.0 8 35.08476922968218 37.0 37.0 37.0 33.0 37.0 9 35.06975432076085 37.0 37.0 37.0 33.0 37.0 10-11 35.033470881675456 37.0 37.0 37.0 33.0 37.0 12-13 34.94464709664655 37.0 37.0 37.0 33.0 37.0 14-15 36.36402109859672 37.0 37.0 40.0 33.0 40.0 16-17 36.36455297966451 37.0 37.0 40.0 33.0 40.0 18-19 36.40175303477806 38.5 37.0 40.0 33.0 40.0 20-21 36.400261789353195 40.0 37.0 40.0 33.0 40.0 22-23 36.358799619857834 38.5 37.0 40.0 33.0 40.0 24-25 36.323720553113915 37.0 37.0 40.0 33.0 40.0 26-27 36.2995852352218 37.0 37.0 40.0 27.0 40.0 28-29 36.24630792221924 37.0 37.0 40.0 27.0 40.0 30-31 36.147006380453064 37.0 37.0 40.0 27.0 40.0 32-33 36.09740030100477 37.0 37.0 40.0 27.0 40.0 34-35 35.99967638455125 37.0 37.0 40.0 27.0 40.0 36-37 35.99204828088125 37.0 37.0 40.0 27.0 40.0 38-39 35.91877481876122 37.0 37.0 40.0 27.0 40.0 40-41 35.856282237892685 37.0 37.0 40.0 27.0 40.0 42-43 35.75122609979792 37.0 37.0 40.0 27.0 40.0 44-45 35.63436541695519 37.0 37.0 40.0 27.0 40.0 46-47 35.508225167107106 37.0 37.0 40.0 27.0 40.0 48-49 35.38114745700437 37.0 37.0 40.0 27.0 40.0 50-51 35.28090103585207 37.0 33.0 40.0 27.0 40.0 52-53 35.17892525048401 37.0 33.0 40.0 27.0 40.0 54-55 35.059534467165044 37.0 33.0 40.0 27.0 40.0 56-57 34.93326720885209 37.0 33.0 40.0 27.0 40.0 58-59 34.80062956629877 37.0 33.0 40.0 27.0 40.0 60-61 34.647921936605286 37.0 33.0 40.0 27.0 40.0 62-63 34.468545355623704 37.0 33.0 40.0 27.0 40.0 64-65 34.28757277814677 37.0 33.0 40.0 27.0 40.0 66-67 34.06148110594519 37.0 33.0 40.0 22.0 40.0 68-69 33.845669170329124 37.0 33.0 37.0 22.0 40.0 70-71 33.62363982589771 37.0 33.0 37.0 22.0 40.0 72-73 33.406526892585106 37.0 33.0 37.0 22.0 40.0 74-75 33.172469016293824 37.0 33.0 37.0 22.0 40.0 76-77 32.94387992312366 37.0 33.0 37.0 22.0 37.0 78-79 32.707408356061784 37.0 33.0 37.0 22.0 37.0 80-81 32.49506663086642 37.0 33.0 37.0 22.0 37.0 82-83 32.29054166725549 37.0 33.0 37.0 22.0 37.0 84-85 32.12829179797352 33.0 33.0 37.0 22.0 37.0 86-87 32.15865759648403 33.0 33.0 37.0 22.0 37.0 88-89 32.16456357842375 33.0 33.0 37.0 22.0 37.0 90-91 32.17207368257422 33.0 33.0 37.0 22.0 37.0 92-93 32.14489369444483 33.0 33.0 37.0 22.0 37.0 94-95 32.17573467066122 33.0 33.0 37.0 22.0 37.0 96-97 32.14149855856874 33.0 33.0 37.0 22.0 37.0 98-99 32.06876916608962 33.0 33.0 37.0 22.0 37.0 100 31.543057812698727 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5092736895137122 1101 2 4.097978414561567 1101 3 2.3287147351191173 1101 4 1.1728586155494014 1101 5 0.5519271364744114 1101 6 0.5458027084369235 1101 7 0.5741616432494538 1101 8 0.5259547828285918 1101 9 0.6028438310924997 1101 10-11 0.6359194030128634 1101 12-13 0.6852712167962594 1101 14-15 0.9041514994508333 1101 16-17 0.9373217545144357 1101 18-19 1.0026855161756245 1101 20-21 1.011005972171283 1101 22-23 0.9491115842829032 1101 24-25 0.9429969034314354 1101 26-27 0.9445028543688991 1101 28-29 0.8746849660102214 1101 30-31 0.7940957024569499 1101 32-33 1.000252735659764 1101 34-35 1.0482050367431768 1101 36-37 0.9382892110205105 1101 38-39 0.9857971726992218 1101 40-41 0.9504428916149408 1101 42-43 1.0193324281740956 1101 44-45 1.0461069067356945 1101 46-47 1.0507463196158042 1101 48-49 0.943455008563042 1101 50-51 0.9783258560201418 1101 52-53 0.9561823395870874 1101 54-55 0.9682412477602824 1101 56-57 0.9428191476930721 1101 58-59 0.976013384636893 1101 60-61 0.9954513276107164 1101 62-63 0.9647390530147888 1101 64-65 1.032680320522232 1101 66-67 0.9753136921585579 1101 68-69 0.9263618197471786 1101 70-71 0.9190290302855075 1101 72-73 0.9151849810205235 1101 74-75 0.7625763359977427 1101 76-77 0.7807087427022168 1101 78-79 0.9293435799907002 1101 80-81 0.8563179738795199 1101 82-83 0.9112903818772509 1101 84-85 0.9649886629942266 1101 86-87 0.8712326521410354 1101 88-89 0.7872719712540288 1101 90-91 0.8538496513186189 1101 92-93 0.9536373079323148 1101 94-95 0.9422611889161843 1101 96-97 0.8909916153756292 1101 98-99 0.8113038826586099 1101 100 1.0203521835319336 1102 1 0.5287234268595498 1102 2 4.09653982581418 1102 3 2.3197433036757893 1102 4 1.051936365114564 1102 5 0.5052520497242412 1102 6 0.4927422520917091 1102 7 0.5173340553848575 1102 8 0.48405756538940636 1102 9 0.5032720098196961 1102 10-11 0.6499001103872786 1102 12-13 0.6386832079857712 1102 14-15 0.8745632732186337 1102 16-17 0.8597520539226977 1102 18-19 0.8293587892572916 1102 20-21 0.839340390792195 1102 22-23 0.8492720830198905 1102 24-25 0.879018026114089 1102 26-27 0.8007111454561411 1102 28-29 0.830410504429409 1102 30-31 0.8057528382592025 1102 32-33 0.946343214774167 1102 34-35 1.0096971942657476 1102 36-37 0.8849687499990821 1102 38-39 0.8423997728006043 1102 40-41 0.8648223602319121 1102 42-43 0.9594903265911796 1102 44-45 0.947235614922981 1102 46-47 0.9392519496361658 1102 48-49 0.827652110626687 1102 50-51 0.8311411120314673 1102 52-53 0.9491777551575353 1102 54-55 0.9376903568775745 1102 56-57 0.8641170514856071 1102 58-59 0.8172193048442153 1102 60-61 0.8539024765587939 1102 62-63 0.92505475698038 1102 64-65 0.8813306670858836 1102 66-67 0.8033949619887224 1102 68-69 0.8001826620760113 1102 70-71 0.8549792177707616 1102 72-73 0.7481681543285603 1102 74-75 0.7758712636258167 1102 76-77 0.8163396535134311 1102 78-79 0.7660180876780345 1102 80-81 0.7429501444255493 1102 82-83 0.7187918950886214 1102 84-85 0.7221092238766822 1102 86-87 0.8510280025942301 1102 88-89 0.8151247570925619 1102 90-91 0.7459884362918316 1102 92-93 0.8278765231601319 1102 94-95 0.7936684754932912 1102 96-97 0.826036252379712 1102 98-99 0.8569189293332506 1102 100 0.8256706571660004 1103 1 0.5374606721992947 1103 2 4.114694703584181 1103 3 2.332422942631446 1103 4 1.051995800272742 1103 5 0.526635267951022 1103 6 0.5489393717725051 1103 7 0.48528257262313446 1103 8 0.49737727527933373 1103 9 0.52413236490667 1103 10-11 0.6020424776799373 1103 12-13 0.6565637696410818 1103 14-15 0.8422858952927683 1103 16-17 0.9067879159056673 1103 18-19 0.8674691945662758 1103 20-21 0.8949647716759657 1103 22-23 0.7763725062702562 1103 24-25 0.7603014237546972 1103 26-27 0.8024857490485715 1103 28-29 0.8161096987989964 1103 30-31 0.7333466148855265 1103 32-33 0.8151342149889089 1103 34-35 0.9986973951538118 1103 36-37 0.878511791998541 1103 38-39 0.8135406091876689 1103 40-41 0.8181036935744856 1103 42-43 0.8438790380674348 1103 44-45 0.8761112652114988 1103 46-47 0.8200762141433984 1103 48-49 0.7952421465787225 1103 50-51 0.78610983583809 1103 52-53 0.9023781098288737 1103 54-55 0.8057210304067652 1103 56-57 0.7818878866950527 1103 58-59 0.861634963029708 1103 60-61 0.8051420251840895 1103 62-63 0.8627661570316505 1103 64-65 0.8656021014959308 1103 66-67 0.9062734801275312 1103 68-69 0.8403482908210194 1103 70-71 0.7886409222713411 1103 72-73 0.840419347534386 1103 74-75 0.8291906964287605 1103 76-77 0.7055728152837446 1103 78-79 0.7648026456203851 1103 80-81 0.7311014893365027 1103 82-83 0.7996765645584887 1103 84-85 0.7330952379111331 1103 86-87 0.7697174504291446 1103 88-89 0.7767793820206919 1103 90-91 0.8262804859735269 1103 92-93 0.8129649485440744 1103 94-95 0.7231330741674284 1103 96-97 0.7517926652116067 1103 98-99 0.7915533476930428 1103 100 0.7985844346235851 1104 1 0.5262732439305537 1104 2 3.7206902893526994 1104 3 2.27835531660514 1104 4 1.1314929310222794 1104 5 0.5051990535959447 1104 6 0.5189911065654798 1104 7 0.5363379270859241 1104 8 0.5160872602709432 1104 9 0.6174428619491366 1104 10-11 0.5833437931209176 1104 12-13 0.6219343002317075 1104 14-15 0.8427781703876747 1104 16-17 0.7869983864627415 1104 18-19 0.8128566758057971 1104 20-21 0.7951544070604442 1104 22-23 0.6748783771981266 1104 24-25 0.779751233942747 1104 26-27 0.741235991831303 1104 28-29 0.7519359546478555 1104 30-31 0.649769674830786 1104 32-33 0.880028008579437 1104 34-35 0.9617287361973368 1104 36-37 0.8308112534623078 1104 38-39 0.7635708025310137 1104 40-41 0.740361308123795 1104 42-43 0.8070364495620908 1104 44-45 0.7972430741635677 1104 46-47 0.8095565708133066 1104 48-49 0.7099429499688554 1104 50-51 0.7145869259766968 1104 52-53 0.673244888322742 1104 54-55 0.6672988533077913 1104 56-57 0.6889658420230376 1104 58-59 0.7132825307881632 1104 60-61 0.7024126175739625 1104 62-63 0.8236235773547733 1104 64-65 0.8029133048154264 1104 66-67 0.7881271042115756 1104 68-69 0.7272962911741558 1104 70-71 0.6646285624231894 1104 72-73 0.6963819148514219 1104 74-75 0.7307833511475721 1104 76-77 0.6792336257366429 1104 78-79 0.6452504252619491 1104 80-81 0.5694894119991645 1104 82-83 0.6667774738901713 1104 84-85 0.7062213966468924 1104 86-87 0.8029820394641938 1104 88-89 0.7570022124259168 1104 90-91 0.7806274445680401 1104 92-93 0.7929797803618506 1104 94-95 0.7659588885931328 1104 96-97 0.8158795372871772 1104 98-99 0.7577586977415969 1104 100 0.6444055699639222 1105 1 0.38799169742841855 1105 2 3.7255045317030557 1105 3 2.11628986483527 1105 4 0.983001926135131 1105 5 0.29942411693807713 1105 6 0.36362377167023396 1105 7 0.27345707390185225 1105 8 0.2324087591383872 1105 9 0.2980246271892355 1105 10-11 0.3150952163311729 1105 12-13 0.22782901624339758 1105 14-15 0.3547108174703979 1105 16-17 0.4162691162135772 1105 18-19 0.416117376827394 1105 20-21 0.25306968938790675 1105 22-23 0.3383421546861385 1105 24-25 0.3830928432746177 1105 26-27 0.35712299724144714 1105 28-29 0.3571880545676436 1105 30-31 0.2806233041494508 1105 32-33 0.3989065719056768 1105 34-35 0.48507865686507046 1105 36-37 0.405902632218222 1105 38-39 0.32740620133024834 1105 40-41 0.2585211811921653 1105 42-43 0.45095449526736786 1105 44-45 0.36769139864490086 1105 46-47 0.38212547327172075 1105 48-49 0.2534267990502599 1105 50-51 0.20976726995009187 1105 52-53 0.1930954538964258 1105 54-55 0.258389002325238 1105 56-57 0.2726616584386292 1105 58-59 0.2148406518325956 1105 60-61 0.2695774555347086 1105 62-63 0.2759435355153883 1105 64-65 0.2412438146975049 1105 66-67 0.2927447862959127 1105 68-69 0.2902528227462824 1105 70-71 0.3306247671958502 1105 72-73 0.3341159874413293 1105 74-75 0.2355444885849849 1105 76-77 0.26955114562271376 1105 78-79 0.30781933478782975 1105 80-81 0.3311769100114432 1105 82-83 0.3715891719540423 1105 84-85 0.3456241253941492 1105 86-87 0.36881916979173823 1105 88-89 0.3602076458910517 1105 90-91 0.3273657470342286 1105 92-93 0.22348185996268555 1105 94-95 0.22290543384080053 1105 96-97 0.26784541557841024 1105 98-99 0.22827795127621542 1105 100 0.19776817672677893 1106 1 0.4526771067960276 1106 2 3.9400718103910215 1106 3 2.2141691253680698 1106 4 0.8967903268118107 1106 5 0.32591071227337665 1106 6 0.18167593823088168 1106 7 0.13321004325221253 1106 8 0.1449252609997913 1106 9 0.22672419517034115 1106 10-11 0.1756526821966915 1106 12-13 0.2327067892289918 1106 14-15 0.3632389810848409 1106 16-17 0.24102472290767452 1106 18-19 0.28035608712527704 1106 20-21 0.2975077415150764 1106 22-23 0.2452417052053022 1106 24-25 0.25518056424857605 1106 26-27 0.20873035731780476 1106 28-29 0.17889623272931487 1106 30-31 0.28659560760907965 1106 32-33 0.28571689092743924 1106 34-35 0.42196921218758376 1106 36-37 0.3207980361831275 1106 38-39 0.27322101417567524 1106 40-41 0.34410733453032094 1106 42-43 0.3957374699416505 1106 44-45 0.3188901181464061 1106 46-47 0.2735250904219768 1106 48-49 0.15124921801471913 1106 50-51 0.1526675471748007 1106 52-53 0.14372243436596932 1106 54-55 0.21110017363408673 1106 56-57 0.12569563766679437 1106 58-59 0.23854461976301877 1106 60-61 0.24384882983532918 1106 62-63 0.27097898620740324 1106 64-65 0.19384149980884047 1106 66-67 0.09153552234293727 1106 68-69 0.19516821111361082 1106 70-71 0.12803147218687627 1106 72-73 0.1338880959968307 1106 74-75 0.0788472930644275 1106 76-77 0.2467233518600409 1106 78-79 0.22860518936926155 1106 80-81 0.1631443471041436 1106 82-83 0.1642255999238671 1106 84-85 0.2275281973084553 1106 86-87 0.3271738792309584 1106 88-89 0.28292998693324023 1106 90-91 0.25724990413128523 1106 92-93 0.29122790919879193 1106 94-95 0.266381561202671 1106 96-97 0.3083341808551445 1106 98-99 0.306112578828138 1106 100 0.4265978722653827 1107 1 0.3955761993219227 1107 2 3.5478027553268134 1107 3 2.1659223684355915 1107 4 0.9518590495712473 1107 5 0.2792033589123193 1107 6 0.20298141265958236 1107 7 0.14958319123791597 1107 8 0.08470878129755732 1107 9 0.14140769723606184 1107 10-11 0.1416791633781287 1107 12-13 0.19044231367541187 1107 14-15 0.14938131743505778 1107 16-17 0.08282864589277494 1107 18-19 0.2010689556791334 1107 20-21 0.24338921346009101 1107 22-23 0.09349134314446417 1107 24-25 0.13812881018851186 1107 26-27 0.07029336364398375 1107 28-29 0.061488437843237875 1107 30-31 0.10103372960552548 1107 32-33 0.18750219056933304 1107 34-35 0.38733714684119036 1107 36-37 0.24880855916753575 1107 38-39 0.1441367572002008 1107 40-41 0.12861975209912657 1107 42-43 0.1155118398181898 1107 44-45 0.1644267722914421 1107 46-47 0.17051050392813494 1107 48-49 0.11385471875947673 1107 50-51 0.03838768704552109 1107 52-53 0.06494582160422624 1107 54-55 0.0121126969831451 1107 56-57 0.07861052842934413 1107 58-59 0.09074317186365732 1107 60-61 0.18042067268201123 1107 62-63 0.26327111082513 1107 64-65 0.05876904894075352 1107 66-67 0.06728830358938609 1107 68-69 0.10498829439620039 1107 70-71 0.13961892406976517 1107 72-73 0.11804298923618006 1107 74-75 0.1520902229880292 1107 76-77 0.09254681667047748 1107 78-79 0.11584054525528131 1107 80-81 0.14577295180250616 1107 82-83 0.21020861660446855 1107 84-85 0.12738160996837777 1107 86-87 0.07172383158685136 1107 88-89 0.005947854334696245 1107 90-91 0.05757378088268439 1107 92-93 0.1323840356810031 1107 94-95 0.03845512491334091 1107 96-97 -0.005359824414949088 1107 98-99 0.057813125273401766 1107 100 0.019293187318513816 1108 1 0.3464406944838565 1108 2 3.6230410697464883 1108 3 2.028719564503419 1108 4 0.9325559293972461 1108 5 0.2144457459837099 1108 6 0.07840483036482482 1108 7 -0.0317760794623112 1108 8 -8.032028806539415E-4 1108 9 -0.027203509554723837 1108 10-11 0.008759310297136835 1108 12-13 -0.02891953268190406 1108 14-15 -0.05930286138808327 1108 16-17 -0.1491017815375031 1108 18-19 -0.0938984183121363 1108 20-21 -0.04025043917982174 1108 22-23 -0.10680343926589586 1108 24-25 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1.0029096057463391 2202 78-79 0.880961339094199 2202 80-81 0.8962167384019679 2202 82-83 0.9141013884464044 2202 84-85 0.9564369741795993 2202 86-87 0.9320046937318089 2202 88-89 0.9903643784634966 2202 90-91 1.0193761108270252 2202 92-93 1.0560708543133082 2202 94-95 1.0280450352589554 2202 96-97 1.060392157745607 2202 98-99 1.0105388817591923 2202 100 0.9592189727431659 2203 1 0.5519139393136854 2203 2 4.0305096955400295 2203 3 2.357189027356835 2203 4 1.0877029179672206 2203 5 0.6245161025226622 2203 6 0.6089986875995947 2203 7 0.5832292620969284 2203 8 0.604643006771802 2203 9 0.6357178721866816 2203 10-11 0.6616456554793047 2203 12-13 0.6923527691148195 2203 14-15 1.0554885335843025 2203 16-17 1.0276687934795703 2203 18-19 1.0878517372835645 2203 20-21 1.0209688712255414 2203 22-23 1.0288219359441442 2203 24-25 1.062256293215512 2203 26-27 0.9869146413852334 2203 28-29 0.987191697298698 2203 30-31 0.9672971377043709 2203 32-33 1.0675095442463558 2203 34-35 1.2337243261774873 2203 36-37 1.03525247944782 2203 38-39 0.9867937990345013 2203 40-41 0.6273363215113505 2203 42-43 0.8773190898339749 2203 44-45 0.9686669855711614 2203 46-47 0.9791762381018074 2203 48-49 1.0699826961477115 2203 50-51 1.000929864519918 2203 52-53 1.0056807522841353 2203 54-55 1.0141538161551296 2203 56-57 0.9326018403850256 2203 58-59 1.0394118003698836 2203 60-61 0.9766032354317389 2203 62-63 1.088912983157094 2203 64-65 0.9963845994362543 2203 66-67 1.0160381758113957 2203 68-69 0.9546244841510259 2203 70-71 0.9444076834292403 2203 72-73 0.9803040605010764 2203 74-75 1.0534696132357553 2203 76-77 1.0107821237048853 2203 78-79 0.921152858822893 2203 80-81 0.9097936333681602 2203 82-83 0.8653027492539067 2203 84-85 0.8453272126230651 2203 86-87 0.8889254442095087 2203 88-89 0.8080195060037312 2203 90-91 0.9375711888148857 2203 92-93 1.0013591960692203 2203 94-95 0.9886378286483648 2203 96-97 0.8863832665539597 2203 98-99 0.963538161749188 2203 100 0.8340258392551334 2204 1 0.4825927926808653 2204 2 4.034744858579977 2204 3 2.35562436270623 2204 4 1.1185850287498198 2204 5 0.5859494651163573 2204 6 0.5921653354824841 2204 7 0.458392582759501 2204 8 0.5843799242217571 2204 9 0.5653303809042853 2204 10-11 0.5277782001893456 2204 12-13 0.5738394542321927 2204 14-15 0.7797174958904165 2204 16-17 0.7870225626114902 2204 18-19 0.8032853125836468 2204 20-21 0.7401343046936191 2204 22-23 0.6932961505575435 2204 24-25 0.7829272133973362 2204 26-27 0.7378828550912644 2204 28-29 0.7760414767654993 2204 30-31 0.8080481399767194 2204 32-33 1.0213478851053068 2204 34-35 0.900305066479838 2204 36-37 0.7844534450200982 2204 38-39 0.7447323382495483 2204 40-41 0.6816950160577591 2204 42-43 0.8618135513778213 2204 44-45 0.915423523612283 2204 46-47 0.9116469576244839 2204 48-49 0.753940111648653 2204 50-51 0.6412091448371129 2204 52-53 0.7169319094912794 2204 54-55 0.7612244734699516 2204 56-57 0.7511602669570294 2204 58-59 0.8009811357609848 2204 60-61 0.7599480047273133 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0.49251089511688306 2205 88-89 0.5122239330364877 2205 90-91 0.466842547445637 2205 92-93 0.5567474904987755 2205 94-95 0.47975106557588987 2205 96-97 0.45608862790657057 2205 98-99 0.5433548708578151 2205 100 0.3380987040217036 2206 1 0.460906522199501 2206 2 3.7581792682037225 2206 3 2.253959221686422 2206 4 1.0103230798379244 2206 5 0.5313053018677962 2206 6 0.45192753201231284 2206 7 0.4243349493550923 2206 8 0.35031650300563655 2206 9 0.3982127613443751 2206 10-11 0.40998909575053943 2206 12-13 0.3996712815216341 2206 14-15 0.5884388650497314 2206 16-17 0.6566206825768077 2206 18-19 0.5612356984010987 2206 20-21 0.4983237903156734 2206 22-23 0.5316725238301032 2206 24-25 0.4608397505365005 2206 26-27 0.5397329183877062 2206 28-29 0.53198165572622 2206 30-31 0.6220094672404102 2206 32-33 0.501449080987932 2206 34-35 0.6023102019498836 2206 36-37 0.3430930035172466 2206 38-39 0.5770214820917232 2206 40-41 0.5837790908486937 2206 42-43 0.5133904733981183 2206 44-45 0.647124744812352 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2210 70-71 0.23594051254707438 2210 72-73 0.2618031933566627 2210 74-75 -0.3279276413624146 2210 76-77 0.1672516514098561 2210 78-79 0.18557139299618086 2210 80-81 0.19065057851227607 2210 82-83 0.21373022810420395 2210 84-85 0.2755635264069234 2210 86-87 0.3823603255931154 2210 88-89 0.3167295932134877 2210 90-91 0.44731882652040156 2210 92-93 0.5144796156675433 2210 94-95 0.4052546843530962 2210 96-97 0.3496645754055585 2210 98-99 0.2984827989641481 2210 100 0.21730599435013787 2211 1 0.39382764504086154 2211 2 3.9520279773214284 2211 3 2.156415135543984 2211 4 0.9442229308949521 2211 5 0.5118337353383069 2211 6 0.3558089631171626 2211 7 0.30075515718129253 2211 8 0.2270829947655173 2211 9 0.27929768197027016 2211 10-11 0.2314572937639241 2211 12-13 0.20958124371058062 2211 14-15 0.12156885503312509 2211 16-17 0.04260193638189236 2211 18-19 0.13799751388211234 2211 20-21 0.10753738578384997 2211 22-23 0.12963822287596116 2211 24-25 0.15727242777494865 2211 26-27 0.07181159314024654 2211 28-29 0.058318627782810495 2211 30-31 0.09165408600004099 2211 32-33 0.19809258960494702 2211 34-35 0.3361996734625805 2211 36-37 0.2291646919689967 2211 38-39 0.08994929929050244 2211 40-41 0.08210415544415639 2211 42-43 0.07092590294075052 2211 44-45 0.2446845348763489 2211 46-47 0.11424997646923174 2211 48-49 0.12162106346949741 2211 50-51 0.08181638470315278 2211 52-53 0.1946201891363728 2211 54-55 0.2035878381175067 2211 56-57 0.12411141672031079 2211 58-59 0.16870007920483232 2211 60-61 0.2224627340795564 2211 62-63 0.1886632147231424 2211 64-65 0.06062605167359436 2211 66-67 0.11707835012114742 2211 68-69 0.1477335373129236 2211 70-71 0.0626857583945224 2211 72-73 0.13570353396088564 2211 74-75 0.18796073250922518 2211 76-77 0.0044501988168406115 2211 78-79 -0.026762918766976895 2211 80-81 0.13005207673043628 2211 82-83 -0.2779396912676475 2211 84-85 -0.0940108505741577 2211 86-87 0.046734691080626334 2211 88-89 0.07318052328576385 2211 90-91 0.1318743982331867 2211 92-93 0.20823681844581543 2211 94-95 0.15465761658121835 2211 96-97 0.07567323961856331 2211 98-99 0.17605685020476258 2211 100 -0.03848244460245809 2212 1 0.623591676827445 2212 2 4.103657658419802 2212 3 2.3776757953285426 2212 4 1.1886786920697432 2212 5 0.6449943686532293 2212 6 0.4856310410990261 2212 7 0.36866740744599014 2212 8 0.38510638874885217 2212 9 0.19612187176910112 2212 10-11 0.20308550752353938 2212 12-13 0.1883373792006111 2212 14-15 0.32687130663972397 2212 16-17 0.316701537035577 2212 18-19 0.36725678832625164 2212 20-21 0.33620325116846317 2212 22-23 0.2820159590311846 2212 24-25 0.21066863369724587 2212 26-27 0.24125829134030852 2212 28-29 0.271075735744418 2212 30-31 0.2209514465165512 2212 32-33 0.3655319467954854 2212 34-35 0.32462531966705654 2212 36-37 0.3915384936423365 2212 38-39 0.4135118827274127 2212 40-41 0.2970482455214949 2212 42-43 0.3386192032445763 2212 44-45 0.5401995492044804 2212 46-47 0.19476785354653003 2212 48-49 0.2176618022601744 2212 50-51 0.36692300412726553 2212 52-53 0.2886776829217581 2212 54-55 0.1156577424239984 2212 56-57 0.25139873891338027 2212 58-59 0.28771420018259874 2212 60-61 0.21010472068361707 2212 62-63 0.2956061366837375 2212 64-65 0.31883397789234635 2212 66-67 0.3470225624767167 2212 68-69 0.25947228340001516 2212 70-71 0.2739487342726221 2212 72-73 0.3582997237712249 2212 74-75 0.3974166878906189 2212 76-77 0.4036934763913038 2212 78-79 0.4273928147873107 2212 80-81 0.28157692222829667 2212 82-83 0.16199832086235233 2212 84-85 0.31353062332447834 2212 86-87 0.16145774527433687 2212 88-89 0.33430586422623776 2212 90-91 0.3354196397398823 2212 92-93 0.3616508626040833 2212 94-95 0.5249232912117918 2212 96-97 0.2716834448158245 2212 98-99 0.356555342586109 2212 100 0.46451624917832746 2213 1 -0.7179745735539491 2213 2 -25.806799072349428 2213 3 -14.722089382062379 2213 4 -6.348170394278021 2213 5 -2.4548550334046055 2213 6 -1.6385129396447766 2213 7 -1.425623908196279 2213 8 -1.376811401619662 2213 9 -1.3805008568134625 2213 10-11 -1.3125381681892492 2213 12-13 -1.4304001989853035 2213 14-15 -1.7141184285452127 2213 16-17 -1.7981295262929038 2213 18-19 -1.715319538291709 2213 20-21 -1.6832294718626883 2213 22-23 -1.6401199255502945 2213 24-25 -1.594841116234953 2213 26-27 -1.6646150813755654 2213 28-29 -1.9207873933449804 2213 30-31 -1.8673072641150341 2213 32-33 -1.6300548660583658 2213 34-35 -1.8536690301820826 2213 36-37 -1.8437827095732473 2213 38-39 -1.6954229242021057 2213 40-41 -1.8087285336848566 2213 42-43 -1.9026847323251417 2213 44-45 -1.603862108548853 2213 46-47 -1.695315910268441 2213 48-49 -1.7233672907408035 2213 50-51 -1.7506531741705231 2213 52-53 -1.8866603281471583 2213 54-55 -1.7610425099321745 2213 56-57 -1.5054642471530002 2213 58-59 -1.5538724442219163 2213 60-61 -1.6230568084549404 2213 62-63 -1.757641465826687 2213 64-65 -1.6224141522141053 2213 66-67 -1.8089461589186797 2213 68-69 -1.6467383852696749 2213 70-71 -1.6794676089148233 2213 72-73 -1.6452248666532263 2213 74-75 -1.785769446504407 2213 76-77 -1.7541002452159766 2213 78-79 -1.7112780669972736 2213 80-81 -1.6806185181978464 2213 82-83 -1.8437016761712464 2213 84-85 -1.7079760659151084 2213 86-87 -1.8136788946805709 2213 88-89 -1.7252212434033467 2213 90-91 -1.664349272140985 2213 92-93 -1.744433945808641 2213 94-95 -1.751531729637783 2213 96-97 -1.788648167297275 2213 98-99 -1.6731055127551073 2213 100 -1.6929505059154124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 35.0 5 32.0 6 36.0 7 39.0 8 46.0 9 89.0 10 362.0 11 1785.0 12 5072.0 13 9363.0 14 12673.0 15 14572.0 16 15777.0 17 16527.0 18 17394.0 19 18717.0 20 21290.0 21 24256.0 22 27343.0 23 27770.0 24 27792.0 25 28922.0 26 31405.0 27 35578.0 28 41936.0 29 50196.0 30 60629.0 31 74960.0 32 95231.0 33 126275.0 34 173333.0 35 244689.0 36 374104.0 37 631260.0 38 617270.0 39 3751.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.36457771584075 20.888191207433866 10.252927325441702 30.49430375128368 2 12.8327867478651 22.588233709620578 36.55807536070525 28.020904181809065 3 15.22059550895242 27.295691251077542 28.974216751692268 28.509496488277776 4 10.388295797578266 18.84553528021798 37.57158079797397 33.19458812422978 5 11.41173989266117 38.99525730248728 34.98279082375439 14.610211981097155 6 29.441769889106205 39.92957743989497 17.80691803912655 12.82173463187227 7 25.718320780383397 33.85984920909524 22.998069257224206 17.42376075329716 8 24.514612863731085 36.84859936492856 20.743169306200006 17.893618465140346 9 24.85807654217836 17.050536838600635 21.964895017993584 36.12649160122742 10-11 22.977436641119116 27.919278252443757 29.031638396712683 20.07164670972444 12-13 23.70429001891426 25.49889170520472 29.4367091482079 21.36010912767312 14-15 21.35136816403972 27.02034732683318 26.697259996055518 24.931024513071588 16-17 20.506811548054042 30.502347913949052 27.566103664456293 21.424736873540617 18-19 20.51532146630903 29.19625471341384 29.882054217953204 20.406369602323927 20-21 21.79288949810706 28.499183615847656 29.15117780707255 20.556749078972732 22-23 21.122907301169647 28.387591154854015 29.307139183322477 21.182362360653862 24-25 21.000033746006356 28.411434136364612 29.21070211304771 21.37783000458133 26-27 20.916800860726443 28.436550133516832 29.25927529024342 21.387373715513306 28-29 20.819767133694942 28.284899026484567 29.580451951447905 21.314881888372586 30-31 21.357170244382523 28.540807870562606 29.068851520521875 21.033170364532992 32-33 20.685243230486375 28.49569190286891 29.51763726627672 21.301427600367994 34-35 20.748342521282556 28.274694096671226 29.586809912350727 21.390153469695488 36-37 21.23815499716826 28.59296505492308 29.02376790948883 21.145112038419835 38-39 21.0365735169523 28.474459378579496 29.2264854502299 21.262481654238304 40-41 21.17819080904539 28.339430345667743 29.090027092074898 21.392351753211972 42-43 20.95550695501938 28.462175788688953 29.13727374546431 21.44504351082736 44-45 21.24120367409685 28.188061976447027 29.011808284701875 21.558926064754242 46-47 21.26783635814976 28.589391505543748 28.83122605594138 21.311546080365112 48-49 21.141393011389827 28.48747147065221 28.767088675140997 21.604046842816967 50-51 21.328988252804816 28.479500980081397 28.35050198472225 21.84100878239154 52-53 21.139587456978916 28.53246455387588 28.477989141670353 21.849958847474856 54-55 21.41381148532332 28.601074665220633 28.19055262161455 21.794561227841502 56-57 21.513141866148704 28.437433360476472 28.274645206097333 21.7747795672775 58-59 21.142381242373347 28.166880349921126 28.76249040015392 21.928248007551616 60-61 21.433082720847064 28.50117103052276 28.334433668015276 21.7313125806149 62-63 21.054237483379076 28.647690102214934 28.62443935849149 21.673633055914504 64-65 20.973864163189614 28.172615110191433 28.655533034815743 22.197987691803203 66-67 21.18928060504153 28.3408090130071 28.458408076878978 22.011502305072398 68-69 21.428018630555243 28.319380442157637 28.454798286780502 21.797802640506617 70-71 21.38866134653401 28.1700105380185 28.31216070372195 22.129167411725543 72-73 21.153995999415514 28.314003300190667 28.59105110842976 21.94094959196406 74-75 21.5064963243388 27.97122867054605 28.28183025770797 22.240444747407178 76-77 21.15521710408802 28.411101219128458 28.371278087542688 22.062403589240827 78-79 21.390602397207754 28.328191041670937 28.446630998054506 21.834575563066803 80-81 21.359726206098742 28.087831138756208 28.287024964683948 22.2654176904611 82-83 20.92759827276169 28.270375015214828 28.55165276736712 22.250373944656367 84-85 20.918991351386683 28.007373978547623 28.48353795505477 22.590096715010922 86-87 21.138450520274368 28.149951656820487 28.33500365952481 22.376594163380336 88-89 21.019783406614042 27.984969225281958 28.52314035147535 22.472107016628648 90-91 21.136219297726484 27.90846930943016 28.42150543713006 22.533805955713294 92-93 20.773944821039084 28.185896134780545 28.602761756827682 22.43739728735269 94-95 20.77150328884844 27.78776628932115 28.789824601815813 22.6509058200146 96-97 20.95980418965735 27.917123300738318 28.558230471430075 22.564842038174255 98-99 20.859990807300598 27.842308816053098 28.561985538279895 22.735714838366412 100 20.900935758803552 27.710810988605232 28.435955094329213 22.952298158262003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 10.0 2 9.0 3 13.0 4 16.5 5 23.5 6 55.5 7 111.0 8 175.5 9 201.5 10 177.0 11 177.0 12 207.5 13 252.0 14 304.0 15 377.0 16 478.5 17 591.5 18 776.0 19 1025.5 20 1379.0 21 1925.5 22 2734.5 23 4077.0 24 6037.0 25 8931.0 26 13282.5 27 19495.0 28 27627.5 29 36908.0 30 46758.0 31 57208.0 32 67444.5 33 77049.5 34 86352.5 35 94563.0 36 101911.0 37 108742.5 38 113571.0 39 116430.5 40 117675.0 41 117050.5 42 114826.0 43 111770.5 44 107659.0 45 104474.0 46 102202.0 47 98704.5 48 94571.5 49 91154.5 50 93838.0 51 90690.5 52 77570.0 53 67969.0 54 61503.5 55 57208.5 56 52370.0 57 46328.0 58 42503.5 59 38751.5 60 34266.0 61 27987.5 62 20458.5 63 15082.5 64 11615.5 65 9126.0 66 7065.0 67 5353.0 68 4027.5 69 2822.0 70 1952.0 71 1400.0 72 1028.5 73 764.0 74 512.5 75 327.5 76 216.0 77 131.5 78 69.0 79 36.5 80 16.5 81 7.5 82 6.0 83 6.0 84 3.0 85 1.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7495089241552789 2 5.566539010499837 3 0.0 4 0.005370038014216469 5 0.16611788646609102 6 0.05642072834673487 7 0.03670703616296652 8 0.04352557127312296 9 0.04161779461017764 10-11 0.04311928550231053 12-13 0.041458813221598854 14-15 0.044302813617285865 16-17 0.0348699178949451 18-19 0.029323233893418874 20-21 0.023440922516004124 22-23 0.02301697214646072 24-25 0.019625369190113477 26-27 0.012453542105337535 28-29 0.020137642553311758 30-31 0.025966960134533586 32-33 0.0012718511086302162 34-35 0.0032326215677684665 36-37 0.0037802241284286987 38-39 0.005864646778683776 40-41 0.006076621963455478 42-43 0.006712547517770586 44-45 0.00773709424416715 46-47 0.017169989966507922 48-49 0.026797196274889423 50-51 0.023069965942653647 52-53 0.028369345561946216 54-55 0.025030736401791896 56-57 0.016304424628690136 58-59 0.014008026793663355 60-61 0.011323007786555121 62-63 0.01777058632336108 64-65 0.018936449839605442 66-67 0.019978661164732982 68-69 0.026850190071082344 70-71 0.02706216525585405 72-73 0.024147506465243135 74-75 0.02434181705128386 76-77 0.017894238514477905 78-79 0.01391970380000848 80-81 0.012789169481226064 82-83 0.007825417237822025 84-85 0.01051043624493026 86-87 0.008284696804827381 88-89 0.0068361997088874134 90-91 0.007507454460664471 92-93 0.003939205517007476 94-95 0.0016251430832497208 96-97 9.185591340107118E-4 98-99 4.592795670053559E-4 100 0.10888458657773131 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2830520.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.37995603139115 #Duplication Level Percentage of deduplicated Percentage of total 1 88.33855259728128 71.88987526243483 2 8.630069049474649 14.046292795882328 3 1.7929685196873217 4.377350978934682 4 0.5326262748365226 1.7338041130943944 5 0.2417472656716729 0.9836690925534887 6 0.11464570471513798 0.559791744534347 7 0.08049976702956008 0.458574725098198 8 0.05261248312495751 0.34252812507290803 9 0.033507957550054084 0.245418850091261 >10 0.161260621728946 2.312137487214103 >50 0.012873955108619824 0.7247451460539153 >100 0.007458024558702035 1.2549452459201134 >500 6.9794324887892E-4 0.40160153680350047 >1k 4.79835983490022E-4 0.6692648963119641 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4059 0.1434012124980569 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3050 0.10775405225894889 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.532919746195046E-5 0.0 5 0.0 0.0 0.0 3.532919746195046E-5 0.0 6 0.0 0.0 0.0 3.532919746195046E-5 0.0 7 0.0 0.0 0.0 7.065839492390091E-5 0.0 8 0.0 0.0 0.0 7.065839492390091E-5 0.0 9 0.0 0.0 0.0 1.0598759238585136E-4 0.0 10-11 0.0 0.0 0.0 1.0598759238585136E-4 0.0 12-13 0.0 0.0 0.0 1.4131678984780183E-4 0.0 14-15 0.0 1.766459873097523E-5 0.0 1.4131678984780183E-4 0.0 16-17 7.065839492390091E-5 5.299379619292569E-5 0.0 1.5898138857877705E-4 0.0 18-19 7.065839492390091E-5 7.065839492390091E-5 0.0 1.7664598730975228E-4 0.0 20-21 1.0598759238585136E-4 7.065839492390091E-5 0.0 1.943105860407275E-4 0.0 22-23 1.4131678984780183E-4 7.065839492390091E-5 0.0 2.2963978350267795E-4 0.0 24-25 1.5898138857877705E-4 7.065839492390091E-5 0.0 2.8263357969560366E-4 0.0 26-27 2.1197518477170273E-4 7.065839492390091E-5 0.0 3.88621172081455E-4 0.0 28-29 2.1197518477170273E-4 7.065839492390091E-5 0.0 8.479007390868108E-4 0.0 30-31 2.1197518477170273E-4 7.065839492390091E-5 0.0 0.0038508825233526 0.0 32-33 2.1197518477170273E-4 7.065839492390091E-5 0.0 0.011499653773864874 0.0 34-35 2.1197518477170273E-4 7.065839492390091E-5 0.0 0.02227505899975976 0.0 36-37 2.1197518477170273E-4 7.065839492390091E-5 0.0 0.03875612961575965 0.0 38-39 2.1197518477170273E-4 7.065839492390091E-5 0.0 0.06447578536805958 0.0 40-41 2.1197518477170273E-4 7.065839492390091E-5 0.0 0.09318075830589431 0.0 42-43 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.12345788053078585 0.0 44-45 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.16097748823537725 0.0 46-47 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.20845992962423865 0.0 48-49 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.2585390670265534 0.0 50-51 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.31163885081186493 0.0 52-53 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.36609880869946154 0.0 54-55 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.4304156126789424 0.0 56-57 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.5072742817574156 0.0 58-59 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.5912694487232029 0.0 60-61 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.6828073993471164 0.0 62-63 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.7775249777426056 0.0 64-65 2.1197518477170273E-4 1.0598759238585136E-4 0.0 0.8840601726891172 0.0 66-67 2.1197518477170273E-4 1.0598759238585136E-4 0.0 1.0078536805957916 0.0 68-69 2.1197518477170273E-4 1.0598759238585136E-4 0.0 1.1427582211042493 0.0 70-71 2.1197518477170273E-4 1.0598759238585136E-4 0.0 1.2968111866370844 0.0 72-73 2.8263357969560366E-4 1.0598759238585136E-4 0.0 1.4605266876757628 0.0 74-75 3.179627771575541E-4 1.0598759238585136E-4 0.0 1.633003829685005 0.0 76-77 3.179627771575541E-4 1.0598759238585136E-4 0.0 1.8263958565917218 0.0 78-79 3.179627771575541E-4 1.236521911168266E-4 0.0 2.0432817998106354 0.0 80-81 3.179627771575541E-4 1.5898138857877705E-4 0.0 2.2814535845003743 0.0 82-83 3.179627771575541E-4 1.7664598730975228E-4 0.0 2.544974068369063 0.0 84-85 3.179627771575541E-4 1.7664598730975228E-4 0.0 2.8176094851829347 0.0 86-87 3.179627771575541E-4 1.7664598730975228E-4 0.0 3.11168972485621 0.0 88 3.179627771575541E-4 1.7664598730975228E-4 0.0 3.359418057459407 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTAC 85 2.501274E-7 41.038334 1 GTATCAA 9335 0.0 24.555803 1 TCGCGGT 160 3.4501696E-5 21.801615 1 TCAACGC 10535 0.0 21.201519 4 ATCAACG 10520 0.0 21.18555 3 CAACGCA 10560 0.0 21.06227 5 AACGCAG 10800 0.0 20.828539 6 TATCAAC 10895 0.0 20.474401 2 TACGACT 200 1.4027726E-5 18.809093 4 GAGTACT 4820 0.0 18.11716 12-13 CGTTACG 65 0.008101266 18.05419 18-19 GGTATCA 7175 0.0 17.224253 1 GTATAGG 900 0.0 17.164446 1 ACGCAGA 13685 0.0 16.15876 7 CGCAGAG 13800 0.0 15.990082 8 GTACATG 11900 0.0 15.9547 1 AGTACTT 5445 0.0 15.778926 12-13 GCAGAGT 13725 0.0 15.632487 9 GTACTTT 5780 0.0 15.432715 14-15 CGTACCC 215 5.17532E-4 15.308645 3 >>END_MODULE