FastQCFastQC Report
Wed 25 May 2016
SRR1295154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423990
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25990.10721991427357373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC1901.983557E-623.5758233
GTATTAG10400.019.6081371
TATACTG10450.018.1386535
TGTACGA2300.002325053616.1185782
CCTACAC8000.016.0978683
TACGACA3209.979944E-615.7483434
TCTATAC9700.015.5855653
TAGCGTG2000.00377328414.9892067
CTATACT11000.014.7620844
GTATAAT11850.014.6014311
CCTATAC6008.62201E-1013.9981453
CTGATCG2150.005952411313.9946389
GTACATG101600.013.8674571
GTCTTAG8950.013.8090141
TACACTG15250.013.7378015
GTGTAGG9000.013.7322991
GTCTAGC4951.1395405E-613.7322981
GTCCTAG8209.094947E-1213.5648311
TACATGG102350.013.5227032
GTATATG12450.013.4013981
Produced by FastQC (version 0.11.5)