##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295154_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2423990 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.52215644453979 33.0 27.0 33.0 15.0 33.0 2 22.79026357369461 27.0 6.0 33.0 2.0 33.0 3 25.057914017797103 33.0 15.0 33.0 15.0 33.0 4 25.265216028118928 33.0 6.0 37.0 2.0 37.0 5 27.063450344267096 33.0 15.0 37.0 2.0 37.0 6 27.923027322719978 33.0 15.0 37.0 6.0 37.0 7 27.61543859504371 33.0 15.0 37.0 6.0 37.0 8 28.418480686801512 33.0 22.0 37.0 6.0 37.0 9 29.498062285735504 33.0 27.0 37.0 15.0 37.0 10-11 28.99614561116176 35.0 24.5 37.0 10.5 37.0 12-13 27.79735312439408 33.0 15.0 37.0 4.0 37.0 14-15 29.60319122603641 33.0 27.0 37.0 10.5 40.0 16-17 30.029736096271023 35.0 27.0 38.5 6.0 40.0 18-19 30.180627807870493 37.0 27.0 40.0 10.5 40.0 20-21 29.725558067483774 33.0 27.0 40.0 6.0 40.0 22-23 30.462562139282753 35.0 27.0 40.0 15.0 40.0 24-25 30.570442741100415 37.0 27.0 40.0 15.0 40.0 26-27 29.19682115025227 33.0 24.5 38.5 4.0 40.0 28-29 29.852026411000047 33.0 27.0 40.0 10.5 40.0 30-31 30.521003799520624 33.0 27.0 40.0 15.0 40.0 32-33 29.303942879302305 33.0 24.5 37.0 6.0 40.0 34-35 28.954916274407076 33.0 22.0 37.0 6.0 40.0 36-37 29.26854978774665 33.0 24.5 37.0 6.0 40.0 38-39 29.386711372571668 33.0 24.5 37.0 6.0 40.0 40-41 28.461079459898762 33.0 22.0 37.0 6.0 40.0 42-43 29.082876373252365 33.0 22.0 37.0 6.0 40.0 44-45 28.975749487415378 33.0 22.0 37.0 6.0 40.0 46-47 28.63801707102752 33.0 22.0 37.0 6.0 40.0 48-49 28.542648278251974 33.0 22.0 37.0 6.0 40.0 50-51 28.511061720551652 33.0 22.0 37.0 6.0 40.0 52-53 28.691985321721624 33.0 22.0 37.0 6.0 40.0 54-55 28.67363561730865 33.0 22.0 37.0 6.0 40.0 56-57 28.680520134158968 33.0 22.0 37.0 6.0 40.0 58-59 28.519507506219085 33.0 22.0 37.0 6.0 40.0 60-61 28.454666067104238 33.0 22.0 37.0 10.5 40.0 62-63 28.385722300834573 33.0 22.0 37.0 10.5 40.0 64-65 27.968110429498473 33.0 22.0 37.0 6.0 40.0 66-67 28.160598434812023 33.0 22.0 37.0 10.5 40.0 68-69 27.737911460030777 33.0 22.0 37.0 6.0 37.0 70-71 27.855420814442304 33.0 22.0 37.0 10.5 37.0 72-73 27.882518698509482 33.0 22.0 37.0 10.5 37.0 74-75 27.97806570984204 33.0 22.0 37.0 15.0 37.0 76-77 27.722770514729845 33.0 22.0 37.0 15.0 37.0 78-79 27.28738361131853 33.0 22.0 35.0 10.5 37.0 80-81 27.09281494560621 27.0 22.0 33.0 10.5 37.0 82-83 27.19218664268417 27.0 22.0 33.0 15.0 37.0 84-85 27.14290343607028 27.0 22.0 33.0 10.5 37.0 86-87 26.92331940313285 27.0 22.0 33.0 15.0 37.0 88-89 26.85817660138862 27.0 22.0 33.0 15.0 37.0 90-91 27.004968048548054 27.0 22.0 33.0 15.0 37.0 92-93 26.939307092851045 27.0 22.0 33.0 15.0 37.0 94-95 26.820736471685116 27.0 22.0 33.0 15.0 37.0 96-97 26.911313371754833 27.0 22.0 33.0 15.0 37.0 98-99 26.980133581409163 27.0 22.0 33.0 15.0 37.0 100 26.66156667312984 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.347452489570994 1101 2 11.25399460194944 1101 3 8.293635889728492 1101 4 12.839714678174914 1101 5 10.74949261819932 1101 6 9.894804207050246 1101 7 9.331755114647219 1101 8 8.983498880098274 1101 9 7.16263555188695 1101 10-11 7.638474129701667 1101 12-13 9.043816994797186 1101 14-15 7.918558345043149 1101 16-17 7.956703377490424 1101 18-19 8.04371721581872 1101 20-21 7.183088232886632 1101 22-23 7.292657689931268 1101 24-25 7.2231986951668254 1101 26-27 8.384178485309093 1101 28-29 8.1287779605799 1101 30-31 5.319879627012401 1101 32-33 6.8592418629868845 1101 34-35 8.607520838578566 1101 36-37 8.075362397451645 1101 38-39 8.111827006447694 1101 40-41 8.7704451758492 1101 42-43 8.518498806911953 1101 44-45 8.381626528784913 1101 46-47 7.144428899289924 1101 48-49 5.433883568044092 1101 50-51 6.65974628499562 1101 52-53 5.7127350121865526 1101 54-55 3.8467767828448274 1101 56-57 5.842207277053692 1101 58-59 5.887625386351335 1101 60-61 6.568812725267524 1101 62-63 6.21204874520015 1101 64-65 6.577155141417634 1101 66-67 5.574078981303071 1101 68-69 6.633722584604669 1101 70-71 6.210386714537847 1101 72-73 5.43048185382764 1101 74-75 4.776994304741457 1101 76-77 5.022052398335532 1101 78-79 5.054105995831591 1101 80-81 4.794084824500274 1101 82-83 4.27009277576477 1101 84-85 4.12572632239144 1101 86-87 3.8849256536400816 1101 88-89 3.8906221238364367 1101 90-91 3.7639026758263974 1101 92-93 3.688204808827564 1101 94-95 3.533800687286565 1101 96-97 3.6477963015079737 1101 98-99 3.439829297451368 1101 100 3.6100252515844815 1102 1 6.767906045514305 1102 2 10.509000264762623 1102 3 8.117972461389485 1102 4 12.67683927804211 1102 5 10.664359342655146 1102 6 9.601243171137451 1102 7 6.6331037827063675 1102 8 7.811069448975054 1102 9 6.367361908237449 1102 10-11 7.4300299370626455 1102 12-13 9.147447459159107 1102 14-15 6.215231540611324 1102 16-17 7.3626312979683455 1102 18-19 7.5434076009462 1102 20-21 7.059018622418513 1102 22-23 6.503883571009943 1102 24-25 7.032426882662119 1102 26-27 8.511972989515193 1102 28-29 7.609829130108114 1102 30-31 4.0885600988460915 1102 32-33 4.211028963682711 1102 34-35 5.0097286053965036 1102 36-37 5.117726511002278 1102 38-39 4.6451172062416255 1102 40-41 6.154468353215481 1102 42-43 7.080739717759723 1102 44-45 6.62297198904016 1102 46-47 5.5833895104355555 1102 48-49 3.6967284771862765 1102 50-51 4.990770369367041 1102 52-53 5.841235366674162 1102 54-55 5.381843353202473 1102 56-57 6.257899091086454 1102 58-59 6.169974733330097 1102 60-61 6.195077974976233 1102 62-63 4.447608992925758 1102 64-65 6.299906469482153 1102 66-67 3.888468271058212 1102 68-69 6.122609875521583 1102 70-71 5.2783598543767525 1102 72-73 4.949815964861475 1102 74-75 4.31413200829094 1102 76-77 3.26320442829676 1102 78-79 4.454001973942123 1102 80-81 4.595258320343611 1102 82-83 4.0493700255251674 1102 84-85 3.117271568156408 1102 86-87 3.2613774166401157 1102 88-89 3.4108596937367857 1102 90-91 3.430294722270453 1102 92-93 3.284350900699966 1102 94-95 3.163095197953215 1102 96-97 3.3984843852749087 1102 98-99 3.228891858896823 1102 100 3.2586347725262144 1103 1 6.830217320873221 1103 2 11.108565064473222 1103 3 8.098947213130067 1103 4 12.27911698941353 1103 5 10.476188434037141 1103 6 9.190895350945471 1103 7 8.29801235628219 1103 8 8.370822124485954 1103 9 7.286590152194897 1103 10-11 7.592302808801531 1103 12-13 8.831438918774882 1103 14-15 8.680909653143129 1103 16-17 8.269826096125403 1103 18-19 7.473253741321276 1103 20-21 8.548291884591812 1103 22-23 7.091261152282087 1103 24-25 5.556678010274208 1103 26-27 6.230936725124884 1103 28-29 7.176556642045991 1103 30-31 6.324598168752903 1103 32-33 8.020792224991169 1103 34-35 7.370457611351654 1103 36-37 4.4319797679951805 1103 38-39 6.663149743927963 1103 40-41 6.8415159990814765 1103 42-43 7.842021877466596 1103 44-45 5.958219794113681 1103 46-47 6.783651233047113 1103 48-49 6.050533293195766 1103 50-51 6.695161739206625 1103 52-53 6.248562478314319 1103 54-55 5.1803679302612196 1103 56-57 6.114706750991129 1103 58-59 5.7194878513438745 1103 60-61 5.195647271619901 1103 62-63 2.8436678375950493 1103 64-65 5.294531885734045 1103 66-67 4.308852146225025 1103 68-69 5.420189494917409 1103 70-71 3.9067496010846767 1103 72-73 4.626799141243435 1103 74-75 3.4140961028472354 1103 76-77 3.788118345453782 1103 78-79 3.09310753120187 1103 80-81 4.2845223698177115 1103 82-83 3.6816739832044085 1103 84-85 3.5366644940543175 1103 86-87 2.988395416521783 1103 88-89 3.174662297692013 1103 90-91 2.8232738007805516 1103 92-93 2.498162965374771 1103 94-95 2.919614934948086 1103 96-97 3.1581382372435485 1103 98-99 2.9914949967356996 1103 100 3.196566557143587 1104 1 6.396297545479332 1104 2 9.76985832887459 1104 3 7.4155585993518685 1104 4 12.287803203432944 1104 5 9.474807563938882 1104 6 8.152162453573357 1104 7 3.713487532215172 1104 8 6.539721779916437 1104 9 6.504284590442019 1104 10-11 7.153559396929911 1104 12-13 7.5503266172571095 1104 14-15 7.315125555210674 1104 16-17 7.118367513185593 1104 18-19 6.954301850208733 1104 20-21 7.529827442308026 1104 22-23 6.419115621065188 1104 24-25 6.019271543373758 1104 26-27 8.252120457876181 1104 28-29 7.174234134815254 1104 30-31 6.354152094772562 1104 32-33 7.718211343704773 1104 34-35 7.986462488146401 1104 36-37 6.638359019548798 1104 38-39 7.300102784020492 1104 40-41 7.166433583637346 1104 42-43 7.2165299347143765 1104 44-45 6.735158879063739 1104 46-47 6.646749008154128 1104 48-49 5.475370162859395 1104 50-51 5.592811982874295 1104 52-53 5.915957337529498 1104 54-55 4.927177958516374 1104 56-57 6.083054904081944 1104 58-59 3.901970065474881 1104 60-61 4.349742515412409 1104 62-63 3.9006495604514377 1104 64-65 2.7120409144015056 1104 66-67 3.226500660418612 1104 68-69 4.1726584436453855 1104 70-71 2.6533626106104755 1104 72-73 2.4659344561889363 1104 74-75 3.1530386165785096 1104 76-77 3.8439638287330524 1104 78-79 4.267457702549404 1104 80-81 3.0314449749738728 1104 82-83 2.945881334552926 1104 84-85 2.8656001953271364 1104 86-87 0.6293882832832338 1104 88-89 0.1860755086627357 1104 90-91 2.132406785841834 1104 92-93 2.4226941567180837 1104 94-95 2.4495534529498855 1104 96-97 2.8438796350320317 1104 98-99 2.6120844259587273 1104 100 0.7370294777537723 1105 1 5.788777569989865 1105 2 -2.7787492515999084 1105 3 0.3824367118883849 1105 4 8.74115472753498 1105 5 8.82604772395839 1105 6 8.220727923804287 1105 7 5.2249740940652885 1105 8 5.943452016815158 1105 9 4.808462608006092 1105 10-11 4.906348602909386 1105 12-13 4.979391547506882 1105 14-15 5.707368350273693 1105 16-17 6.005078975518504 1105 18-19 6.094183937273474 1105 20-21 6.899635371520809 1105 22-23 1.9950733735438746 1105 24-25 3.921078791771034 1105 26-27 6.868358688893956 1105 28-29 5.922625447832221 1105 30-31 5.266634356646438 1105 32-33 7.111485720203817 1105 34-35 6.115972790100983 1105 36-37 6.100542683980368 1105 38-39 4.039456810068216 1105 40-41 5.199212460052685 1105 42-43 5.51283684787381 1105 44-45 6.1381808925631525 1105 46-47 5.43966643683331 1105 48-49 5.002417381492624 1105 50-51 5.81729854245112 1105 52-53 5.556504602031968 1105 54-55 5.187637430523559 1105 56-57 5.217118673117412 1105 58-59 2.090144203182504 1105 60-61 4.341898855558224 1105 62-63 -0.8640476654746649 1105 64-65 -0.6822648235371709 1105 66-67 0.6314323709705256 1105 68-69 2.6239294251600427 1105 70-71 2.8480659113704085 1105 72-73 2.6499987495491446 1105 74-75 2.4998485624982614 1105 76-77 1.565962834056016 1105 78-79 3.056161906907871 1105 80-81 1.3576229805114757 1105 82-83 0.857919607032688 1105 84-85 1.1866469682476612 1105 86-87 -0.36759276116725914 1105 88-89 1.539538292275676 1105 90-91 1.4202405744577113 1105 92-93 1.6397531612915905 1105 94-95 1.0341043563394194 1105 96-97 1.9800110035335479 1105 98-99 1.8989939913800455 1105 100 2.0317854896502965 1106 1 6.2302449863916785 1106 2 10.415337402571172 1106 3 6.867133204975065 1106 4 11.975344606110482 1106 5 10.017247245866415 1106 6 9.385755386928725 1106 7 6.648397123973201 1106 8 7.510675226007564 1106 9 6.895494037120805 1106 10-11 5.690234621435451 1106 12-13 8.072293891787435 1106 14-15 7.943076007894643 1106 16-17 7.785535933658284 1106 18-19 7.3016233201001945 1106 20-21 8.337248117018326 1106 22-23 6.145083152697978 1106 24-25 5.01209522618279 1106 26-27 7.82869710195456 1106 28-29 6.417861152610417 1106 30-31 5.60496391413972 1106 32-33 7.544251047669661 1106 34-35 6.903940790535614 1106 36-37 5.971790015086231 1106 38-39 7.201006425693134 1106 40-41 6.66923353956264 1106 42-43 6.262721182212221 1106 44-45 4.707054605653639 1106 46-47 6.306823310763715 1106 48-49 5.913778368100612 1106 50-51 6.692433087312992 1106 52-53 5.760251756569765 1106 54-55 5.342580195307736 1106 56-57 4.959598014654365 1106 58-59 5.135506656663612 1106 60-61 5.116321803973186 1106 62-63 5.1239561918583405 1106 64-65 5.41795911909179 1106 66-67 3.0252007136755488 1106 68-69 3.3557242802384124 1106 70-71 3.0275829841436135 1106 72-73 2.9184566312757063 1106 74-75 2.922358654042011 1106 76-77 3.593573841037241 1106 78-79 4.220261110993384 1106 80-81 3.7183417483380587 1106 82-83 2.3074213048024994 1106 84-85 2.7602450450140203 1106 86-87 3.3463618200947955 1106 88-89 3.478495834448278 1106 90-91 3.317669526186716 1106 92-93 3.0420936388402815 1106 94-95 1.6892267859159276 1106 96-97 1.6553618306869993 1106 98-99 2.1333195828830505 1106 100 2.973806514831619 1107 1 6.10279957331241 1107 2 10.456667173247627 1107 3 6.222680135782152 1107 4 11.637980864241165 1107 5 9.377150550320636 1107 6 8.085257550613019 1107 7 3.775218424342963 1107 8 6.392360578629624 1107 9 5.84884907027331 1107 10-11 4.5815872974765846 1107 12-13 6.2824318637720005 1107 14-15 6.192698726129866 1107 16-17 6.987990010471325 1107 18-19 6.194128731560074 1107 20-21 5.447248045045463 1107 22-23 5.756388200694886 1107 24-25 4.4960324298570065 1107 26-27 7.138969627212539 1107 28-29 6.389550877584103 1107 30-31 5.033520894158247 1107 32-33 4.917568996802835 1107 34-35 6.510848872188831 1107 36-37 6.332029792690076 1107 38-39 6.457029151125642 1107 40-41 7.630027526229664 1107 42-43 6.2633279854474 1107 44-45 4.2194421435518485 1107 46-47 3.2868020247885283 1107 48-49 3.989487597721048 1107 50-51 4.837985901901888 1107 52-53 3.881611827822905 1107 54-55 5.099506702014708 1107 56-57 5.148945562628779 1107 58-59 2.7550223872331863 1107 60-61 2.6282099941254984 1107 62-63 0.24364827394339272 1107 64-65 2.084450675774921 1107 66-67 3.968457179987748 1107 68-69 4.775442290548526 1107 70-71 3.952873151750424 1107 72-73 2.023663508283075 1107 74-75 2.4075242905970278 1107 76-77 2.947177756361164 1107 78-79 3.856396851819092 1107 80-81 1.9138124958662388 1107 82-83 1.3313110243782198 1107 84-85 2.1876491996477228 1107 86-87 2.1330602447886875 1107 88-89 2.446649964341308 1107 90-91 1.2777028856102177 1107 92-93 2.151356006927511 1107 94-95 2.1669558440610075 1107 96-97 2.086606241544107 1107 98-99 1.6167319244302867 1107 100 -0.012124769074347341 1108 1 4.214417152359246 1108 2 -3.2707392403171394 1108 3 -0.09419150759183736 1108 4 6.758719636167392 1108 5 6.791673742255131 1108 6 6.6723064213316405 1108 7 4.019366799892943 1108 8 4.901286677021481 1108 9 4.744740058476797 1108 10-11 3.181293212367617 1108 12-13 2.4372311863985665 1108 14-15 3.8031346611670607 1108 16-17 4.395420087656529 1108 18-19 3.774970777953733 1108 20-21 4.481403166133539 1108 22-23 3.935475718177507 1108 24-25 2.8343407933455076 1108 26-27 4.532410247732791 1108 28-29 5.915427317883331 1108 30-31 4.319981912661365 1108 32-33 4.423341147146893 1108 34-35 2.8002045254292334 1108 36-37 1.1305539681707408 1108 38-39 2.3626095142073886 1108 40-41 5.198958138135939 1108 42-43 2.8577018300165093 1108 44-45 3.323910056418196 1108 46-47 4.578799995509055 1108 48-49 4.640006978714485 1108 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-18.237247121515498 2213 54-55 -18.14331509265038 2213 56-57 -17.99635411698363 2213 58-59 -18.42777332339124 2213 60-61 -19.77436730098817 2213 62-63 -17.91242924818155 2213 64-65 -18.010367482753136 2213 66-67 -8.976249797418944 2213 68-69 -23.20084714833302 2213 70-71 -17.818219558863117 2213 72-73 -9.620182112179787 2213 74-75 -4.5029113534374225 2213 76-77 -3.232808108601251 2213 78-79 -4.93841453099116 2213 80-81 -3.494729804890561 2213 82-83 -3.7120207296028873 2213 84-85 -3.281166573036341 2213 86-87 -3.5662584740961805 2213 88-89 -2.9773176783914117 2213 90-91 -2.828177893409393 2213 92-93 -3.926747049267423 2213 94-95 -3.9635490804515605 2213 96-97 -3.6582332827510413 2213 98-99 -3.8280780735617306 2213 100 -3.477337838197549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 645.0 3 584.0 4 3012.0 5 11068.0 6 18138.0 7 20486.0 8 18093.0 9 17931.0 10 20836.0 11 26283.0 12 32099.0 13 43591.0 14 34491.0 15 37987.0 16 44090.0 17 46754.0 18 47091.0 19 48133.0 20 48303.0 21 47461.0 22 49179.0 23 54705.0 24 60799.0 25 67229.0 26 73815.0 27 79411.0 28 85748.0 29 89946.0 30 95684.0 31 104161.0 32 118832.0 33 144006.0 34 172636.0 35 200719.0 36 217154.0 37 185983.0 38 56810.0 39 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.26480699691836 20.713274194410303 14.119729599743494 23.90218920892784 2 14.795817490494297 21.00472569255839 40.81803367734927 23.381423139598045 3 17.76560944202057 26.70008501466382 29.802616031424296 25.73168951189131 4 12.269443153416386 17.82209163879671 39.45616481246147 30.452300395325434 5 13.299296937756658 37.72263950511625 35.922942801588555 13.055120755538537 6 30.305263263101367 37.950564742034054 18.627855397690876 13.1163165971737 7 26.13962893089351 31.769892637481544 24.059108606387937 18.031369825237007 8 23.903767745383764 36.24134624760295 22.22317020761783 17.631715799395455 9 24.45138615692844 17.424986517503797 22.236435653538493 35.88719167202927 10-11 23.083396221828625 27.465031970927285 29.295512578988138 20.156059228255955 12-13 24.083537973304324 25.05569228740791 29.18216345194211 21.67860628734565 14-15 21.53582057222714 26.646497380468738 26.477792361616743 25.339889685687382 16-17 21.069642967631232 30.077440144100454 27.47850789735791 21.374408990910403 18-19 21.18640963515118 29.214076712256222 28.89481744318959 20.70469620940301 20-21 22.365647168680265 28.63147337180647 27.976955396721316 21.025924062791944 22-23 21.470333870417253 28.616609047045316 28.28124842949932 21.631808653038107 24-25 21.71833758020807 28.50558590556755 27.879061305090637 21.897015209133748 26-27 21.731569467734925 28.341333427974007 27.99316494118059 21.933932163110477 28-29 21.56490818606072 28.265221466583597 28.466485796887415 21.70338455046827 30-31 22.12014135796531 28.70024660814269 27.7749764722618 21.404635561630194 32-33 21.25132664342398 28.546454752825625 28.319610577394037 21.88260802635636 34-35 21.41730212989745 28.048607735696873 28.27171708318172 22.262373051223957 36-37 22.026602365720326 28.509252357864646 27.817952365178932 21.6461929112361 38-39 21.79799893538039 28.26269576393461 28.021164567778445 21.91814073290655 40-41 21.847302908662463 28.30913918576486 27.751002636810796 22.092555268761885 42-43 21.72548470748544 28.338641216992972 28.123751150921706 21.812122924599883 44-45 22.117938101914238 27.990051787234314 28.036491725317326 21.855518385534122 46-47 21.900868922409362 28.32654248490376 28.23860045097357 21.533988141713305 48-49 21.81783683248557 28.114831510057943 28.29321055480814 21.77412110264835 50-51 21.949774908767925 28.20349321863948 28.025212507876983 21.82151936471561 52-53 21.468983951221237 28.203931911971143 28.08329213505443 22.24379200175319 54-55 21.91217210016862 28.327451694481777 27.788365859095883 21.97201034625373 56-57 22.046449312356337 28.133009228185745 27.78203928824713 22.03850217121079 58-59 21.77124767487951 27.677208637428645 28.235152082378445 22.316391605313402 60-61 22.160349404164762 28.108338683301522 27.80101472377179 21.930297188761926 62-63 21.59123547170293 28.29479332214258 28.15607906712536 21.957892139029127 64-65 21.644403157782303 27.857923532642175 28.202875752681383 22.294797556894135 66-67 21.877331479671557 28.030651379876563 27.883846779463507 22.20817036098837 68-69 22.302476984606916 27.94780876788042 27.815362001626177 21.934352245886487 70-71 22.20990494079917 28.02211359615729 27.656372797663387 22.111608665380153 72-73 22.041054556512112 28.100159748289638 27.977374716545217 21.881410978653037 74-75 22.30185242431279 27.684998683031857 27.730526074202167 22.282622818453188 76-77 22.171236821634515 28.049373485918217 27.899490732406196 21.879898960041068 78-79 22.235885249866637 27.88361030332131 27.994536746796754 21.885967700015293 80-81 22.25953043491088 27.792698487289417 27.858930281711658 22.088840796088046 82-83 21.780658124971293 28.053124032907288 28.066487968197574 22.099729873923845 84-85 22.03616535970773 27.773164653847545 27.666502265374604 22.524167721070125 86-87 22.281723107322062 27.879360274774186 27.743203996956534 22.09571262094722 88-89 22.082631605847514 27.52394391831331 28.12166406752208 22.271760408317096 90-91 22.302734737555298 27.67107656350465 27.784440208541096 22.24174849039896 92-93 21.85042185042185 27.86609226551339 28.06803081766902 22.21545506639573 94-95 21.77357852083808 27.559230631103617 28.05065068576943 22.61654016228888 96-97 22.126900462883736 27.675427542567565 27.953759335720875 22.243912658827824 98-99 22.093239398618334 27.52275821080852 27.923266249048567 22.460736141524585 100 22.141460886811657 27.45170164230364 27.77940018449871 22.627437286385994 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 271.0 1 341.0 2 335.0 3 254.0 4 292.0 5 379.5 6 526.0 7 777.0 8 1197.0 9 1755.0 10 2441.0 11 3214.5 12 4043.0 13 4883.5 14 5687.0 15 6426.0 16 7101.5 17 7771.5 18 8285.0 19 8800.0 20 9627.0 21 10443.5 22 11287.0 23 12499.0 24 14210.0 25 16547.5 26 19587.0 27 23932.5 28 29654.5 29 36239.0 30 43203.5 31 50265.5 32 57652.0 33 64256.5 34 70303.5 35 76093.5 36 81158.5 37 85579.0 38 88834.5 39 90861.0 40 91746.0 41 91543.0 42 89833.5 43 87112.5 44 84392.5 45 81641.0 46 78922.5 47 76563.0 48 74498.0 49 73417.0 50 73818.5 51 70542.0 52 62531.0 53 55389.0 54 50279.0 55 46155.0 56 42127.5 57 37941.0 58 33874.5 59 29809.0 60 25796.5 61 22078.5 62 18482.0 63 15086.0 64 12059.0 65 9634.5 66 7661.5 67 5937.0 68 4609.5 69 3583.5 70 2689.5 71 2012.0 72 1528.0 73 1109.0 74 808.0 75 582.5 76 395.5 77 276.5 78 197.5 79 132.0 80 86.5 81 67.0 82 53.0 83 29.0 84 16.5 85 16.5 86 11.0 87 5.0 88 7.0 89 6.0 90 4.5 91 3.0 92 1.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 1.5 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.361003964537807 2 24.05084179390179 3 5.90790391049468 4 23.84151749800948 5 12.076865003568496 6 9.19178709483125 7 7.698752882643905 8 8.612494275966485 9 4.9919347852095095 10-11 6.657927631714652 12-13 9.887252010115553 14-15 7.247843431697326 16-17 7.654796430678344 18-19 6.5231292208301195 20-21 7.575897590336594 22-23 5.598496693468207 24-25 5.174815077619957 26-27 9.331866055553034 28-29 6.141630122236478 30-31 4.153008056963931 32-33 8.61443322785985 34-35 6.807288808947232 36-37 6.654173490814731 38-39 5.75934719202637 40-41 8.10599466169415 42-43 6.266444993584957 44-45 6.147240706438558 46-47 5.685873291556484 48-49 3.7903415443133017 50-51 6.3173940486553155 52-53 4.37015004187311 54-55 4.789685601013206 56-57 4.484011897738852 58-59 3.4677329526936993 60-61 4.060701570551033 62-63 3.0460109158866167 64-65 4.4083308924541775 66-67 2.4315900643154467 68-69 4.967966039463859 70-71 2.442274926876761 72-73 1.5051217207991783 74-75 0.7774165735007158 76-77 1.1186721067331136 78-79 1.6678492898073012 80-81 2.114798328375942 82-83 0.753365319163858 84-85 1.2564614540489027 86-87 0.4518995540410646 88-89 0.7190211180739194 90-91 0.32473318784318417 92-93 0.32625959678051475 94-95 0.5202579218561132 96-97 0.5593051126448542 98-99 0.4612023976996605 100 0.4076336948584771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2423990.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.57612224062238 #Duplication Level Percentage of deduplicated Percentage of total 1 96.86330261689771 91.60955548926117 2 2.4358214692443116 4.607410980631649 3 0.38325682703330205 1.0874083356916393 4 0.12197566417153811 0.46143941300273966 5 0.0630072857549061 0.29794923798029127 6 0.04024838753324274 0.2283921851599144 7 0.024208245854004665 0.16026654133835697 8 0.01284922080099697 0.09721835817374698 9 0.010008855791816118 0.08519388919700063 >10 0.04270554194559262 0.6623816444424162 >50 9.437725253345331E-4 0.058675385310393555 >100 0.001276654440213826 0.24836562196587736 >500 3.0757844990658E-4 0.19972072489909645 >1k 8.787955710985313E-5 0.19602219294585305 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2599 0.10721991427357373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 6 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 7 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 8 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 9 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 10-11 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 12-13 0.0 8.250859120705944E-5 0.0 4.125429560352972E-5 0.0 14-15 0.0 1.2376288681058915E-4 0.0 4.125429560352972E-5 0.0 16-17 0.0 1.2376288681058915E-4 0.0 4.125429560352972E-5 0.0 18-19 0.0 1.4439003461235402E-4 0.0 8.250859120705944E-5 0.0 20-21 0.0 1.6501718241411888E-4 0.0 1.0313573900882429E-4 0.0 22-23 0.0 1.6501718241411888E-4 0.0 1.6501718241411888E-4 0.0 24-25 0.0 1.8564433021588372E-4 0.0 2.475257736211783E-4 0.0 26-27 0.0 2.0627147801764858E-4 0.0 2.475257736211783E-4 0.0 28-29 0.0 2.0627147801764858E-4 0.0 4.331701038370621E-4 0.0 30-31 0.0 2.0627147801764858E-4 0.0 0.001877070449960602 0.0 32-33 0.0 2.0627147801764858E-4 0.0 0.006064381453718869 0.0 34-35 0.0 2.0627147801764858E-4 0.0 0.012685695898085388 0.0 36-37 0.0 2.0627147801764858E-4 0.0 0.022607353990734286 0.0 38-39 0.0 2.0627147801764858E-4 0.0 0.040140429622234415 0.0 40-41 0.0 2.0627147801764858E-4 0.0 0.06289217364758105 0.0 42-43 0.0 2.0627147801764858E-4 0.0 0.08287987986749121 0.0 44-45 0.0 2.0627147801764858E-4 0.0 0.10936513764495728 0.0 46-47 0.0 2.0627147801764858E-4 0.0 0.14042962223441516 0.0 48-49 0.0 2.0627147801764858E-4 0.0 0.17465006043754305 0.0 50-51 0.0 2.0627147801764858E-4 0.0 0.21085070482964038 0.0 52-53 0.0 2.0627147801764858E-4 0.0 0.24913469114971595 0.0 54-55 0.0 2.0627147801764858E-4 0.0 0.2917091242125586 0.0 56-57 0.0 2.0627147801764858E-4 0.0 0.34218375488347724 0.0 58-59 0.0 2.0627147801764858E-4 0.0 0.3982895969042777 0.0 60-61 0.0 2.0627147801764858E-4 0.0 0.45891278429366456 0.0 62-63 0.0 2.2689862581941344E-4 0.0 0.5237026555390081 0.0 64-65 0.0 2.475257736211783E-4 0.0 0.5978572518863527 0.0 66-67 0.0 2.475257736211783E-4 0.0 0.6889054822833428 0.0 68-69 0.0 2.475257736211783E-4 0.0 0.7892565563389289 0.0 70-71 0.0 2.475257736211783E-4 0.0 0.9061506029315303 0.0 72-73 0.0 2.475257736211783E-4 0.0 1.0322031031481154 0.0 74-75 2.062714780176486E-5 2.475257736211783E-4 0.0 1.1605245896228944 0.0 76-77 4.125429560352972E-5 2.475257736211783E-4 0.0 1.3059459816253367 0.0 78-79 4.125429560352972E-5 2.475257736211783E-4 0.0 1.4693748736587198 2.062714780176486E-5 80-81 4.125429560352972E-5 2.6815292142294317E-4 0.0 1.6536990664152906 4.125429560352972E-5 82-83 4.125429560352972E-5 2.8878006922470803E-4 0.0 1.8566908279324585 4.125429560352972E-5 84-85 4.125429560352972E-5 2.8878006922470803E-4 0.0 2.069212331734042 4.125429560352972E-5 86-87 4.125429560352972E-5 2.8878006922470803E-4 0.0 2.2950796001633673 4.125429560352972E-5 88 4.125429560352972E-5 2.8878006922470803E-4 0.0 2.483962392584128 4.125429560352972E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGAC 190 1.983557E-6 23.575823 3 GTATTAG 1040 0.0 19.608137 1 TATACTG 1045 0.0 18.138653 5 TGTACGA 230 0.0023250536 16.118578 2 CCTACAC 800 0.0 16.097868 3 TACGACA 320 9.979944E-6 15.748343 4 TCTATAC 970 0.0 15.585565 3 TAGCGTG 200 0.003773284 14.989206 7 CTATACT 1100 0.0 14.762084 4 GTATAAT 1185 0.0 14.601431 1 CCTATAC 600 8.62201E-10 13.998145 3 CTGATCG 215 0.0059524113 13.994638 9 GTACATG 10160 0.0 13.867457 1 GTCTTAG 895 0.0 13.809014 1 TACACTG 1525 0.0 13.737801 5 GTGTAGG 900 0.0 13.732299 1 GTCTAGC 495 1.1395405E-6 13.732298 1 GTCCTAG 820 9.094947E-12 13.564831 1 TACATGG 10235 0.0 13.522703 2 GTATATG 1245 0.0 13.401398 1 >>END_MODULE