FastQCFastQC Report
Wed 25 May 2016
SRR1295154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423990
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT72470.2989698802387799No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53420.22038044711405574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG13850.016.3003565
GTACTAG5403.6379788E-1215.6353291
GCGTAGA1950.003318213415.28158951
CATGGGG42200.015.2691114
GAGTACT31400.014.72971912-13
TATACTG12250.014.5899275
TAGACTC7600.014.23376755
CTAGTAC6105.456968E-1213.8775373
GTACATG141450.013.8339161
AGTACTT37700.013.82513912-13
TCTGTCG5101.0550139E-913.8110568
GTATCAA117750.013.4549361
CTGTCGC4554.8950824E-813.4161779
GTACTGA9700.013.3123121
TACATGG141600.013.2835772
TAGGACC6451.4551915E-1113.1255784
ACTAGAC7950.013.0144333
TATACGT2550.001875452812.9111074
ACATGGG137800.012.8665433
GTATAGA10100.012.7850921
Produced by FastQC (version 0.11.5)