##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295154_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2423990 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.429524874277533 33.0 33.0 33.0 27.0 33.0 2 30.12839945709347 33.0 33.0 33.0 27.0 33.0 3 30.9313681162051 33.0 33.0 33.0 27.0 33.0 4 35.21204006617189 37.0 37.0 37.0 33.0 37.0 5 35.50388697973177 37.0 37.0 37.0 33.0 37.0 6 35.26474696677791 37.0 37.0 37.0 33.0 37.0 7 35.16781298602717 37.0 37.0 37.0 33.0 37.0 8 35.15532778600572 37.0 37.0 37.0 33.0 37.0 9 35.14252534045107 37.0 37.0 37.0 33.0 37.0 10-11 35.10750374382732 37.0 37.0 37.0 33.0 37.0 12-13 35.018996571768035 37.0 37.0 37.0 33.0 37.0 14-15 36.46462320389111 37.0 37.0 40.0 33.0 40.0 16-17 36.45321309081308 37.0 37.0 40.0 33.0 40.0 18-19 36.484087599371286 38.5 37.0 40.0 33.0 40.0 20-21 36.48080850168524 40.0 37.0 40.0 33.0 40.0 22-23 36.44288219010805 40.0 37.0 40.0 33.0 40.0 24-25 36.41457019212125 40.0 37.0 40.0 33.0 40.0 26-27 36.38339143313297 37.0 37.0 40.0 33.0 40.0 28-29 36.330409778918224 37.0 37.0 40.0 33.0 40.0 30-31 36.2408988898469 37.0 37.0 40.0 30.0 40.0 32-33 36.19929413900222 37.0 37.0 40.0 30.0 40.0 34-35 36.10836038927553 37.0 37.0 40.0 27.0 40.0 36-37 36.08898695951716 37.0 37.0 40.0 27.0 40.0 38-39 36.02662263458183 37.0 37.0 40.0 27.0 40.0 40-41 35.97014509135764 37.0 37.0 40.0 27.0 40.0 42-43 35.874378813443954 37.0 37.0 40.0 27.0 40.0 44-45 35.77069583620394 37.0 37.0 40.0 27.0 40.0 46-47 35.66785650930903 37.0 37.0 40.0 27.0 40.0 48-49 35.55044265859182 37.0 37.0 40.0 27.0 40.0 50-51 35.45285273454098 37.0 37.0 40.0 27.0 40.0 52-53 35.35525249691624 37.0 33.0 40.0 27.0 40.0 54-55 35.2318400653468 37.0 33.0 40.0 27.0 40.0 56-57 35.10033519115178 37.0 33.0 40.0 27.0 40.0 58-59 34.96837342563295 37.0 33.0 40.0 27.0 40.0 60-61 34.80000515678695 37.0 33.0 40.0 27.0 40.0 62-63 34.618424993502444 37.0 33.0 40.0 27.0 40.0 64-65 34.43213792136106 37.0 33.0 40.0 27.0 40.0 66-67 34.20136613599891 37.0 33.0 38.5 27.0 40.0 68-69 33.979312414655176 37.0 33.0 37.0 27.0 40.0 70-71 33.75069142199432 37.0 33.0 37.0 27.0 40.0 72-73 33.52897144790201 37.0 33.0 37.0 22.0 40.0 74-75 33.296511124220814 37.0 33.0 37.0 22.0 40.0 76-77 33.057183197950486 37.0 33.0 37.0 22.0 37.0 78-79 32.82735489832879 37.0 33.0 37.0 22.0 37.0 80-81 32.6236886290785 37.0 33.0 37.0 22.0 37.0 82-83 32.420064645481204 37.0 33.0 37.0 22.0 37.0 84-85 32.2454968873634 33.0 33.0 37.0 22.0 37.0 86-87 32.27400628715465 33.0 33.0 37.0 22.0 37.0 88-89 32.291867128164725 33.0 33.0 37.0 22.0 37.0 90-91 32.31166774615407 33.0 33.0 37.0 22.0 37.0 92-93 32.28097846938312 33.0 33.0 37.0 22.0 37.0 94-95 32.31599099006184 33.0 33.0 37.0 22.0 37.0 96-97 32.27642090107632 33.0 33.0 37.0 22.0 37.0 98-99 32.20223453892136 33.0 33.0 37.0 22.0 37.0 100 31.663315442720474 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5416196441847383 1101 2 4.032444010853876 1101 3 2.3298017199805052 1101 4 1.1506682673055408 1101 5 0.436004468532623 1101 6 0.488380259600639 1101 7 0.5533467772997653 1101 8 0.5371098816972548 1101 9 0.6283076882839111 1101 10-11 0.6272745383048814 1101 12-13 0.629764498340883 1101 14-15 0.9339506995623879 1101 16-17 0.9455594229044024 1101 18-19 0.9916189541107272 1101 20-21 1.0095050743877536 1101 22-23 0.950979125807315 1101 24-25 0.9372109960906627 1101 26-27 0.9452602911102801 1101 28-29 0.8389955282851176 1101 30-31 0.7445956081995035 1101 32-33 0.9638814589171218 1101 34-35 1.0661549859204484 1101 36-37 0.9726559311788634 1101 38-39 1.0099875453392855 1101 40-41 0.9441870594029993 1101 42-43 1.0682282165844725 1101 44-45 1.061930595455074 1101 46-47 1.0459647733953048 1101 48-49 0.9581897179462686 1101 50-51 0.9877830200081092 1101 52-53 0.9495613184109146 1101 54-55 0.8970423265035166 1101 56-57 0.911522831058484 1101 58-59 0.8935979927784459 1101 60-61 0.9232649182229764 1101 62-63 0.9752759975715151 1101 64-65 0.921561467791733 1101 66-67 0.9591116263469175 1101 68-69 0.8726774446678363 1101 70-71 0.8323328458563921 1101 72-73 0.8201303334288141 1101 74-75 0.7693334590708929 1101 76-77 0.7599157794030731 1101 78-79 0.8278725396535975 1101 80-81 0.8349619055745308 1101 82-83 0.8212671075035374 1101 84-85 0.9842481176153584 1101 86-87 0.9348210359391729 1101 88-89 0.9435810693746873 1101 90-91 0.8398618247996978 1101 92-93 0.8420046829215337 1101 94-95 0.9136701400929539 1101 96-97 0.8772421631353993 1101 98-99 0.8255589777868764 1101 100 0.9334435026096308 1102 1 0.5099773412849053 1102 2 3.948169005432632 1102 3 2.209628594073923 1102 4 1.0576970094531646 1102 5 0.33383071791887176 1102 6 0.4335845365975928 1102 7 0.47810425384234634 1102 8 0.47178570811405507 1102 9 0.4879921802655218 1102 10-11 0.5130819512531772 1102 12-13 0.6183650320947294 1102 14-15 0.7593713797291741 1102 16-17 0.7370392836823356 1102 18-19 0.7171311142717087 1102 20-21 0.7276990456826908 1102 22-23 0.772968551290198 1102 24-25 0.845638131471226 1102 26-27 0.6987541850471288 1102 28-29 0.7140413841507964 1102 30-31 0.7263276987101293 1102 32-33 0.7614641876739867 1102 34-35 0.9105604509284149 1102 36-37 0.7247748513792658 1102 38-39 0.7420188927742188 1102 40-41 0.7485056145541975 1102 42-43 0.8399641778076798 1102 44-45 0.8148186372003394 1102 46-47 0.7506555616049724 1102 48-49 0.7928359592540133 1102 50-51 0.7354786047553716 1102 52-53 0.7591701517253426 1102 54-55 0.7182184599369279 1102 56-57 0.6441009377126292 1102 58-59 0.7061428655168314 1102 60-61 0.7266769597907228 1102 62-63 0.7983615748694817 1102 64-65 0.6846449608598348 1102 66-67 0.6622393123911152 1102 68-69 0.6486365344942087 1102 70-71 0.7032313792551932 1102 72-73 0.677871182760903 1102 74-75 0.5722422448019486 1102 76-77 0.6352904913467796 1102 78-79 0.6408803951215347 1102 80-81 0.6319273006175479 1102 82-83 0.7035277483115223 1102 84-85 0.6966541376689079 1102 86-87 0.6934335821040207 1102 88-89 0.588572892564585 1102 90-91 0.6796397447720111 1102 92-93 0.6999908225121274 1102 94-95 0.6867423819714773 1102 96-97 0.6341549173299796 1102 98-99 0.7133331719876566 1102 100 0.701130734988336 1103 1 0.5478560207841241 1103 2 3.9920077225935664 1103 3 2.305120935024739 1103 4 1.0933658992211903 1103 5 0.4754693720610632 1103 6 0.5157136111501472 1103 7 0.4551817426711864 1103 8 0.4550892634251511 1103 9 0.5595203603774976 1103 10-11 0.5415639291934511 1103 12-13 0.5839966857357766 1103 14-15 0.7212869511462117 1103 16-17 0.8154259642832002 1103 18-19 0.8456360826232299 1103 20-21 0.8185132549070744 1103 22-23 0.7478852793449065 1103 24-25 0.7724786422149279 1103 26-27 0.7532316107709605 1103 28-29 0.8037012022677388 1103 30-31 0.7527097887053031 1103 32-33 0.8266746595690719 1103 34-35 0.9417288131319239 1103 36-37 0.8054985310068119 1103 38-39 0.703678446048535 1103 40-41 0.7882656724457462 1103 42-43 0.7685081213044711 1103 44-45 0.8311399600447302 1103 46-47 0.780913239687024 1103 48-49 0.7746070002070695 1103 50-51 0.7447621694797348 1103 52-53 0.7271503067876779 1103 54-55 0.7438656727233948 1103 56-57 0.6737165844775461 1103 58-59 0.7562941148170736 1103 60-61 0.8052924661545333 1103 62-63 0.745129031748256 1103 64-65 0.6980054954393751 1103 66-67 0.7499146619039507 1103 68-69 0.789141861289103 1103 70-71 0.7315091178010178 1103 72-73 0.6782665775759256 1103 74-75 0.7359403351240772 1103 76-77 0.5794976589633407 1103 78-79 0.6718641798363905 1103 80-81 0.6362527940864524 1103 82-83 0.7160063727286499 1103 84-85 0.757822991599781 1103 86-87 0.7200643754378362 1103 88-89 0.6534527425043422 1103 90-91 0.6747098004658909 1103 92-93 0.7077646440252963 1103 94-95 0.6518097532940104 1103 96-97 0.6829045998459122 1103 98-99 0.7006136263207452 1103 100 0.6847949622606997 1104 1 0.4200515471281818 1104 2 3.5770834961835902 1104 3 2.1739425591034127 1104 4 1.0101758894015518 1104 5 0.4884140315995893 1104 6 0.40243770826693037 1104 7 0.32451953641727016 1104 8 0.2730164997204412 1104 9 0.3466750028182446 1104 10-11 0.4569101999984895 1104 12-13 0.4581228967794999 1104 14-15 0.6465136114651244 1104 16-17 0.7038192565011272 1104 18-19 0.6285364897379182 1104 20-21 0.5583272785988171 1104 22-23 0.6390065386890598 1104 24-25 0.6132974852231214 1104 26-27 0.6603370327600757 1104 28-29 0.5582999412160703 1104 30-31 0.5932402701002388 1104 32-33 0.735387588178618 1104 34-35 0.7934274173467628 1104 36-37 0.7651331953493496 1104 38-39 0.6702081618473201 1104 40-41 0.6287531732612379 1104 42-43 0.6624674717807864 1104 44-45 0.6130007057497338 1104 46-47 0.7228788587380635 1104 48-49 0.5807309969089118 1104 50-51 0.4640049885927766 1104 52-53 0.4704022093739155 1104 54-55 0.5339602197472502 1104 56-57 0.6174152288173431 1104 58-59 0.5914183056271227 1104 60-61 0.5790167941363293 1104 62-63 0.6488426489989507 1104 64-65 0.5383988343721455 1104 66-67 0.5675865591436136 1104 68-69 0.5531614014174053 1104 70-71 0.5725350107811309 1104 72-73 0.4963880136017522 1104 74-75 0.5220621806003223 1104 76-77 0.4441779907206964 1104 78-79 0.4535156057262668 1104 80-81 0.5334905852771357 1104 82-83 0.5873186188768855 1104 84-85 0.5863241460347126 1104 86-87 0.6282373010262958 1104 88-89 0.6682531636115101 1104 90-91 0.5205224083612379 1104 92-93 0.6606630544101719 1104 94-95 0.6406881988702295 1104 96-97 0.5587004819256549 1104 98-99 0.5526195929331124 1104 100 0.499923774764401 1105 1 0.4517236950050645 1105 2 3.7195661789839107 1105 3 2.229759641486268 1105 4 0.9224363765747725 1105 5 0.32276712156264153 1105 6 0.3253155148617921 1105 7 0.27847370398134075 1105 8 0.26030540126156865 1105 9 0.2999969374049627 1105 10-11 0.29088933275362905 1105 12-13 0.39606781657504797 1105 14-15 0.44412024500179825 1105 16-17 0.3819824398548448 1105 18-19 0.40057182379914025 1105 20-21 0.3460589888867389 1105 22-23 0.30868367271093433 1105 24-25 0.39008747306979075 1105 26-27 0.3887770009134002 1105 28-29 0.3335061750046364 1105 30-31 0.2732118054998196 1105 32-33 0.37104347113525904 1105 34-35 0.5433964345209219 1105 36-37 0.4182583688256756 1105 38-39 0.3526596982911556 1105 40-41 0.24420280886287316 1105 42-43 0.4406834762692924 1105 44-45 0.4977581234232318 1105 46-47 0.3455144749666701 1105 48-49 0.3410926071857432 1105 50-51 0.30118970717376214 1105 52-53 0.2893036874343764 1105 54-55 0.2541559512745053 1105 56-57 0.25072664684298474 1105 58-59 0.25485773515593024 1105 60-61 0.3062556002980159 1105 62-63 0.37228382236586555 1105 64-65 0.22058346018798147 1105 66-67 0.3177831992559774 1105 68-69 0.32671799222710973 1105 70-71 0.32522026846328345 1105 72-73 0.26055988813310904 1105 74-75 0.26013665260713736 1105 76-77 0.18591102113365565 1105 78-79 0.24136092581991875 1105 80-81 0.28560663894950267 1105 82-83 0.28244862098078727 1105 84-85 0.3207975886930541 1105 86-87 0.29183734625755875 1105 88-89 0.23278421159294282 1105 90-91 0.308882080029818 1105 92-93 0.31537053363499723 1105 94-95 0.26187994208282106 1105 96-97 0.2596044361188916 1105 98-99 0.2918353145795862 1105 100 0.1148984748121471 1106 1 0.5081770131263816 1106 2 3.9356960244189487 1106 3 2.2895607986861712 1106 4 0.9971608489079173 1106 5 0.38339700774415775 1106 6 0.36561453966281476 1106 7 0.1844858192666976 1106 8 0.21217827225822816 1106 9 0.3128257750438337 1106 10-11 0.2485862876336924 1106 12-13 0.309760859793478 1106 14-15 0.4005599266360065 1106 16-17 0.3265771210815913 1106 18-19 0.37418179853632694 1106 20-21 0.3489459038352649 1106 22-23 0.3037795355854698 1106 24-25 0.28513536376058113 1106 26-27 0.31153314684010525 1106 28-29 0.3102524874486079 1106 30-31 0.31202983174055277 1106 32-33 0.3994176324369718 1106 34-35 0.4260238215852752 1106 36-37 0.3599049067334832 1106 38-39 0.3478658482925141 1106 40-41 0.23056681071005158 1106 42-43 0.31828177035018257 1106 44-45 0.33109623767634844 1106 46-47 0.2270333405847964 1106 48-49 0.2082977792716889 1106 50-51 0.21332862549066078 1106 52-53 0.15088122797711634 1106 54-55 0.14662714351637618 1106 56-57 0.23801255127892063 1106 58-59 0.21236752789219793 1106 60-61 0.2570093014362058 1106 62-63 0.2766533114050631 1106 64-65 0.3264587759731583 1106 66-67 0.21916825344879243 1106 68-69 0.12034230972364668 1106 70-71 0.149689070245266 1106 72-73 0.13789202623649288 1106 74-75 0.11029089159880101 1106 76-77 0.14134703558507056 1106 78-79 0.16086936034033528 1106 80-81 0.15367378914100982 1106 82-83 0.2638667613116539 1106 84-85 0.25813532264573524 1106 86-87 0.27533149234724874 1106 88-89 0.3790949170502529 1106 90-91 0.2858503792719276 1106 92-93 0.2901524856628086 1106 94-95 0.2906574934912882 1106 96-97 0.27537353942478404 1106 98-99 0.17587535478277516 1106 100 0.2847841130377766 1107 1 0.38387900947375897 1107 2 3.4574342770976862 1107 3 2.0752052707202147 1107 4 0.8143624359096151 1107 5 0.22867146727291754 1107 6 0.15745490384551175 1107 7 -0.03773350004991727 1107 8 0.005098157461503661 1107 9 0.09314202381804648 1107 10-11 0.07140854164394028 1107 12-13 0.030276347454631036 1107 14-15 0.016207421530438637 1107 16-17 -0.02711449317983039 1107 18-19 0.015044177419490268 1107 20-21 0.017232355842857316 1107 22-23 0.09180159526896858 1107 24-25 0.06335513290760986 1107 26-27 0.027209898704647628 1107 28-29 0.02691220873368394 1107 30-31 -0.019166339302962854 1107 32-33 0.0467831736279436 1107 34-35 0.24289496316490045 1107 36-37 0.011948000487130628 1107 38-39 -0.01660784112657865 1107 40-41 -0.07351187362595368 1107 42-43 0.13160029526629557 1107 44-45 0.18296387957535387 1107 46-47 0.13864549680190663 1107 48-49 -0.02278679784274118 1107 50-51 -0.04745308980267282 1107 52-53 0.01388560932535654 1107 54-55 -0.0012465261101723968 1107 56-57 0.011972444399212634 1107 58-59 0.10357830942755442 1107 60-61 0.084914297252773 1107 62-63 0.10054223927925676 1107 64-65 -0.06593214464976427 1107 66-67 -0.04391531604550636 1107 68-69 -0.05820214701615356 1107 70-71 -0.002302059098973075 1107 72-73 0.016961704967620506 1107 74-75 0.0011146390460154976 1107 76-77 -0.040999333833653395 1107 78-79 -0.06431020296429324 1107 80-81 -0.0728854220363715 1107 82-83 0.06040193355170942 1107 84-85 0.019849050570307725 1107 86-87 0.056574733645284425 1107 88-89 0.15098936884193392 1107 90-91 0.15423671162463393 1107 92-93 0.1735358581313875 1107 94-95 0.0348548790769172 1107 96-97 0.03543446445604559 1107 98-99 0.00677019342860774 1107 100 0.056801062647771516 1108 1 0.4299579494769823 1108 2 3.5328157978566566 1108 3 2.0510065582130963 1108 4 0.95818422650283 1108 5 0.23021247168922798 1108 6 -0.09284522860956201 1108 7 -0.1730068201305599 1108 8 -0.054414512302763285 1108 9 -0.13089729174038922 1108 10-11 -0.025954861386225048 1108 12-13 -0.09485515096466912 1108 14-15 -0.17572126352848016 1108 16-17 -0.17582240869478483 1108 18-19 -0.16485028888251918 1108 20-21 -0.11268744641899531 1108 22-23 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-0.3441742433088564 2211 78-79 -0.1879759317622529 2211 80-81 -0.3043852177410642 2211 82-83 -0.7553745435058055 2211 84-85 -0.5047333623525674 2211 86-87 -0.3049765038259409 2211 88-89 -0.25448425771751104 2211 90-91 -0.2869593605669749 2211 92-93 -0.14712301351936574 2211 94-95 -0.21424878061913688 2211 96-97 -0.22999472894542805 2211 98-99 -0.14266138564249076 2211 100 -0.2788211087009529 2212 1 0.5921801356868812 2212 2 4.2326858760973245 2212 3 2.485485857234181 2212 4 1.1707877029282159 2212 5 0.7432596150821738 2212 6 0.7545794108571897 2212 7 0.5970634929591299 2212 8 0.4749792090263796 2212 9 0.33147058378700933 2212 10-11 0.44060346171644227 2212 12-13 0.43565157017599176 2212 14-15 0.609835492600098 2212 16-17 0.6352672069520011 2212 18-19 0.791240346545699 2212 20-21 0.7298108530936531 2212 22-23 0.5865523693091177 2212 24-25 0.4237567533000046 2212 26-27 0.4119125379408075 2212 28-29 0.3876186076671857 2212 30-31 0.5091445858389108 2212 32-33 0.6854394903604728 2212 34-35 0.523764649063807 2212 36-37 0.46259187004339 2212 38-39 0.37949114103255965 2212 40-41 0.3770414399059874 2212 42-43 0.6060553847139687 2212 44-45 0.8038425296357516 2212 46-47 0.7542011782116518 2212 48-49 0.7127567956651291 2212 50-51 0.6469039572627082 2212 52-53 0.5052934059630729 2212 54-55 0.5624319834851477 2212 56-57 0.45872137251233625 2212 58-59 0.49566495177978 2212 60-61 0.5575932202496361 2212 62-63 0.7554995253004577 2212 64-65 0.8182964028271797 2212 66-67 0.7319270356501946 2212 68-69 0.6930167827915668 2212 70-71 0.635801482423247 2212 72-73 0.6370052190546076 2212 74-75 0.5375851929258886 2212 76-77 0.8453735773493207 2212 78-79 0.7302932479281594 2212 80-81 0.5571304636140866 2212 82-83 0.5328737870727664 2212 84-85 0.7975435974310585 2212 86-87 0.807189415844519 2212 88-89 0.660959085668523 2212 90-91 0.7444311755248592 2212 92-93 0.6584772319547696 2212 94-95 0.8772648315084624 2212 96-97 0.6715515253732605 2212 98-99 0.6469588832761417 2212 100 0.4432540583929665 2213 1 -0.5215542259661134 2213 2 -25.911732547009624 2213 3 -14.80503406543749 2213 4 -6.220765852662765 2213 5 -2.208773523295484 2213 6 -1.5980394119701984 2213 7 -1.1712927895590326 2213 8 -1.1486075666846816 2213 9 -1.1617930232608273 2213 10-11 -1.1589195712543656 2213 12-13 -1.0543388659607018 2213 14-15 -1.474286246388175 2213 16-17 -1.6070324525908077 2213 18-19 -1.2835190242011478 2213 20-21 -1.3820992220684971 2213 22-23 -1.3458983502285449 2213 24-25 -1.5433752321008 2213 26-27 -1.532267401651545 2213 28-29 -1.5264500804895604 2213 30-31 -1.6611254911215738 2213 32-33 -1.4420398594603085 2213 34-35 -1.3589086142162685 2213 36-37 -1.720523415380704 2213 38-39 -1.6961575872597194 2213 40-41 -1.4037627829074992 2213 42-43 -1.3600956916293683 2213 44-45 -1.256805033827014 2213 46-47 -1.2633525980838627 2213 48-49 -1.4123898298033595 2213 50-51 -1.6206255006624204 2213 52-53 -1.836163897926646 2213 54-55 -1.6369365563091023 2213 56-57 -1.5230899169334933 2213 58-59 -1.4466516693154787 2213 60-61 -1.569209097965441 2213 62-63 -1.5073875761541515 2213 64-65 -1.5417626152011081 2213 66-67 -1.6100603678426566 2213 68-69 -1.7735928685361344 2213 70-71 -1.5266360357133522 2213 72-73 -1.4393246279644494 2213 74-75 -1.6474044462221933 2213 76-77 -1.5161328946068977 2213 78-79 -1.5380356508788111 2213 80-81 -1.5822728575805023 2213 82-83 -1.6583252107651418 2213 84-85 -1.5140556521735107 2213 86-87 -1.4854488616791741 2213 88-89 -1.6022419066844726 2213 90-91 -1.5780359333683123 2213 92-93 -1.4707565145865047 2213 94-95 -1.6203711776630385 2213 96-97 -1.6373230449681344 2213 98-99 -1.5283510226792032 2213 100 -1.489187465122285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 23.0 5 37.0 6 31.0 7 38.0 8 40.0 9 81.0 10 344.0 11 1455.0 12 4217.0 13 7641.0 14 10247.0 15 11777.0 16 12637.0 17 13104.0 18 13734.0 19 14783.0 20 16477.0 21 18453.0 22 20234.0 23 21544.0 24 22419.0 25 23817.0 26 26358.0 27 29919.0 28 35694.0 29 42437.0 30 51792.0 31 63828.0 32 81393.0 33 109234.0 34 149983.0 35 214717.0 36 328594.0 37 553136.0 38 520599.0 39 3160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.08691553669874 20.488759617750528 10.127316739042248 31.297008106508486 2 12.692834544803796 22.550636620689716 36.164243870876724 28.592284963629762 3 15.324980713616807 26.989385269741213 28.530191956237445 29.15544206040454 4 10.573031454968673 18.787332198497758 36.934112150689444 33.70552419584413 5 11.734653822092678 38.59004162613827 34.94442823767806 14.73087631409099 6 30.069470816478166 39.308841740279036 17.605258812845705 13.016428630397096 7 26.19510916778266 33.1106988760289 22.969206864745853 17.72498509144259 8 24.581387180796245 36.92063742316679 20.57968346758467 17.918291928452298 9 24.793892209835395 17.00665785116327 21.698148249033018 36.501301689968315 10-11 23.131419458590965 27.75637026763389 28.763342950376103 20.34886732339905 12-13 24.006014039637687 25.126527189261893 29.12019701221256 21.747261758887856 14-15 21.602358125443804 26.605594893644767 26.255813439986063 25.53623354092536 16-17 20.88695146903581 30.059377158859903 27.364294634183384 21.689376737920906 18-19 20.985590245323607 29.073956923833684 29.22790059295445 20.71255223788826 20-21 22.00005942008473 28.36787056985099 28.55817989678236 21.073890113281916 22-23 21.243123611079305 28.36271227494868 28.585641074523004 21.80852303944901 24-25 21.406397095429092 28.225672846664757 28.439104671547096 21.928825386359055 26-27 21.359559988967128 28.149685610623408 28.546089158718825 21.944665241690636 28-29 21.160941321617532 28.097127819905655 28.95223142490791 21.789699433568906 30-31 21.787477240020426 28.511350330890128 28.331333721907864 21.36983870718158 32-33 20.857969369080955 28.413572688613158 28.921688347478252 21.806769594827628 34-35 21.02722027071544 28.08901153734843 28.9622079693124 21.921560222623732 36-37 21.710418018661763 28.334922378807263 28.38455340533614 21.570106197194836 38-39 21.45963198020329 28.357661839471877 28.595755407644987 21.58695077267985 40-41 21.48511746977389 28.190724127047652 28.466962206273994 21.857196196904464 42-43 21.37290476431145 28.293311851935133 28.541021803550414 21.792761580203006 44-45 21.6937998264699 27.926195659932162 28.46291773988391 21.917086773714026 46-47 21.59369164754379 28.401284793304782 28.449271645814946 21.555751913336486 48-49 21.57056729108926 28.293084199769293 28.458121912506286 21.67822659663516 50-51 21.65536012009333 28.23315618219244 28.140560857802427 21.970922839911804 52-53 21.26287051176268 28.435941510831935 28.071936832127655 22.22925114527773 54-55 21.624009373593793 28.4508130816845 27.88456400265164 22.040613542070066 56-57 21.74501548519025 28.125879371482 27.996939271482248 22.132165871845505 58-59 21.32243479609907 27.822607264811268 28.586132030163046 22.26882590892662 60-61 21.861103294733724 28.239342885175724 27.91846798636457 21.981085833725984 62-63 21.170099010186963 28.403448098720112 28.23710042155303 22.189352469539887 64-65 21.15830727599977 27.96894741576446 28.413613853011054 22.459131455224714 66-67 21.52480957726452 28.13798037911862 28.1195152692017 22.217694774415158 68-69 21.711767550802456 28.095541301394622 28.06126996650859 22.131421181294332 70-71 21.661764293224657 28.016527766085808 27.91897548648216 22.402732454207374 72-73 21.492868598125405 28.17644950702556 28.20176516355214 22.1289167312969 74-75 21.82761152057018 27.787967831799048 27.94854886982558 22.435871777805197 76-77 21.53865737498032 28.19682128763908 28.166885596765212 22.097635740615388 78-79 21.679488369694155 28.131559131466293 28.234689772551448 21.9542627262881 80-81 21.631319943374827 28.03909546606636 28.01801162458219 22.311572965976623 82-83 21.130198321882247 28.27082165223264 28.140653448508466 22.458326577376642 84-85 21.339179454024205 27.94069683508646 27.925699200256798 22.79442451063254 86-87 21.507689451372343 28.112427927509774 27.90966570741921 22.470216913698675 88-89 21.31748140785991 27.767129925594308 28.29970131691618 22.615687349629603 90-91 21.5146976217819 27.87094948223056 28.028490676148156 22.58586221983938 92-93 20.95467182859366 28.194839640727903 28.204823546836277 22.645664983842163 94-95 20.9463416304466 27.768537830005542 28.356131125344504 22.928989414203354 96-97 21.36391492693681 27.90433931864073 28.109127880844742 22.622617873577717 98-99 21.22368499490609 27.85702896504438 28.100657866102907 22.81862817394662 100 21.19697998384435 27.779787597813538 28.066310845924992 22.95692157241712 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7.0 1 7.0 2 6.5 3 9.0 4 11.0 5 21.5 6 55.0 7 118.0 8 199.0 9 226.0 10 186.0 11 172.5 12 188.0 13 231.0 14 282.5 15 315.5 16 378.5 17 479.0 18 605.0 19 787.0 20 1052.5 21 1458.5 22 2088.5 23 3078.5 24 4618.0 25 6855.0 26 10168.5 27 14874.0 28 21217.5 29 28814.5 30 36707.0 31 44867.5 32 53437.5 33 61702.0 34 69208.5 35 76186.5 36 82425.5 37 87752.5 38 92350.0 39 95862.0 40 97911.5 41 98433.5 42 97005.0 43 94879.5 44 92506.5 45 89253.0 46 86452.0 47 84043.0 48 81445.0 49 81183.0 50 89898.5 51 86147.0 52 68625.0 53 60398.0 54 55775.5 55 51336.5 56 47455.0 57 43046.0 58 38224.5 59 34042.0 60 29744.0 61 25035.0 62 20767.0 63 16776.5 64 13242.0 65 10516.5 66 8181.0 67 6209.0 68 4668.0 69 3436.5 70 2509.5 71 1852.5 72 1373.5 73 954.0 74 675.0 75 442.0 76 235.0 77 133.5 78 77.0 79 40.5 80 24.5 81 12.5 82 6.5 83 5.0 84 3.5 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7434849153668125 2 5.252991967788645 3 0.0 4 0.005940618566908279 5 0.15990164975928117 6 0.0573434708889063 7 0.036510051609123795 8 0.04319324749689561 9 0.0406767354650803 10-11 0.042677568801851494 12-13 0.04096551553430501 14-15 0.04325512894030091 16-17 0.03448859112455084 18-19 0.028919261218074335 20-21 0.02339118560720135 22-23 0.023535575641813702 24-25 0.020606520653963095 26-27 0.013345764627741865 28-29 0.01990519762870309 30-31 0.024855713101126657 32-33 0.0014026460505200103 34-35 0.003279716500480613 36-37 0.00424919244716356 38-39 0.006311907227340047 40-41 0.007219501730617701 42-43 0.007735180425661822 44-45 0.008374622007516533 46-47 0.01689363404964542 48-49 0.02493822169233371 50-51 0.022339201069311344 52-53 0.027619750906563146 54-55 0.02419564437147018 56-57 0.01584164951175541 58-59 0.014934055008477758 60-61 0.012541305863473035 62-63 0.01862631446499367 64-65 0.020132096254522502 66-67 0.020647774949566624 68-69 0.028135429601607264 70-71 0.02780539523677903 72-73 0.024278152962677237 74-75 0.02487634024892842 76-77 0.019038857421028964 78-79 0.015057817895288346 80-81 0.013737680435975397 82-83 0.008374622007516533 84-85 0.011324304143168908 86-87 0.00886967355475889 88-89 0.007570163243247704 90-91 0.007405146060833584 92-93 0.003671632308714145 94-95 0.0014232731983217753 96-97 0.0010932388334935375 98-99 6.806958774582403E-4 100 0.10503343660658666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2423990.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.12086733752709 #Duplication Level Percentage of deduplicated Percentage of total 1 89.02527621376267 73.1083289763711 2 8.045721683457211 13.214432860037093 3 1.6157660845820505 3.9806433684131433 4 0.5505499779105808 1.8084656679469309 5 0.253017358734109 1.0389002475347633 6 0.13524644837355798 0.6663933386853996 7 0.08436929760382371 0.4849935927118764 8 0.05653199032271501 0.37139648620944354 9 0.03805092301304162 0.28122973207420027 >10 0.18291086414478536 2.620933165062 >50 0.008667905808450262 0.48537914701863366 >100 0.0025801878968830867 0.39820660915853395 >500 7.059577485133013E-4 0.3574916828029228 >1k 5.04255534641212E-4 0.6618958714019244 >5k 1.0085110692824238E-4 0.5213092545719986 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7247 0.2989698802387799 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5342 0.22038044711405574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.125429560352972E-5 0.0 2 0.0 0.0 0.0 4.125429560352972E-5 0.0 3 0.0 0.0 0.0 4.125429560352972E-5 0.0 4 0.0 0.0 0.0 4.125429560352972E-5 0.0 5 0.0 0.0 0.0 4.125429560352972E-5 0.0 6 0.0 0.0 0.0 4.125429560352972E-5 0.0 7 0.0 0.0 0.0 4.125429560352972E-5 0.0 8 0.0 0.0 0.0 4.125429560352972E-5 0.0 9 0.0 0.0 0.0 4.125429560352972E-5 0.0 10-11 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 12-13 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 14-15 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 16-17 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 18-19 0.0 4.125429560352972E-5 0.0 4.125429560352972E-5 0.0 20-21 0.0 4.125429560352972E-5 0.0 6.188144340529458E-5 0.0 22-23 0.0 4.125429560352972E-5 0.0 8.250859120705944E-5 0.0 24-25 0.0 4.125429560352972E-5 0.0 2.0627147801764858E-4 0.0 26-27 0.0 6.188144340529458E-5 0.0 2.0627147801764858E-4 0.0 28-29 0.0 8.250859120705944E-5 0.0 5.363058428458863E-4 0.0 30-31 0.0 8.250859120705944E-5 0.0 0.0027021563620311965 0.0 32-33 0.0 8.250859120705944E-5 0.0 0.008560266337732417 0.0 34-35 0.0 1.0313573900882429E-4 0.0 0.01788373714413013 0.0 36-37 0.0 1.2376288681058915E-4 0.0 0.032116469127347884 0.0 38-39 0.0 1.2376288681058915E-4 0.0 0.05618835061200747 0.0 40-41 0.0 1.2376288681058915E-4 0.0 0.0866959022108177 0.0 42-43 0.0 1.2376288681058915E-4 0.0 0.1145219245953985 0.0 44-45 0.0 1.2376288681058915E-4 0.0 0.15099072190891877 0.0 46-47 0.0 1.2376288681058915E-4 0.0 0.1939983250755985 0.0 48-49 0.0 1.6501718241411888E-4 0.0 0.23910989731805826 0.0 50-51 0.0 1.6501718241411888E-4 0.0 0.28855317059888863 0.0 52-53 0.0 1.6501718241411888E-4 0.0 0.3415649404494243 0.0 54-55 0.0 1.6501718241411888E-4 0.0 0.40123927903993 0.0 56-57 0.0 1.6501718241411888E-4 0.0 0.4705671228016617 0.0 58-59 0.0 1.6501718241411888E-4 0.0 0.549404081700007 0.0 60-61 0.0 1.6501718241411888E-4 0.0 0.63160326569004 0.0 62-63 0.0 1.6501718241411888E-4 0.0 0.71914488096073 0.0 64-65 0.0 1.6501718241411888E-4 0.0 0.8184027161828226 0.0 66-67 0.0 1.6501718241411888E-4 0.0 0.935998085800684 0.0 68-69 0.0 2.0627147801764858E-4 0.0 1.0647321152314984 0.0 70-71 0.0 2.0627147801764858E-4 0.0 1.2123399849009278 0.0 72-73 0.0 2.0627147801764858E-4 0.0 1.367683034996019 0.0 74-75 0.0 2.475257736211783E-4 0.0 1.528203499189353 0.0 76-77 0.0 2.475257736211783E-4 0.0 1.709371738332254 0.0 78-79 0.0 2.475257736211783E-4 0.0 1.9104451751038578 0.0 80-81 0.0 2.475257736211783E-4 0.0 2.1344147459354206 0.0 82-83 0.0 2.475257736211783E-4 0.0 2.3802078391412502 0.0 84-85 0.0 2.475257736211783E-4 0.0 2.6370364564210247 0.0 86-87 0.0 2.475257736211783E-4 0.0 2.910222401907599 0.0 88 0.0 2.475257736211783E-4 0.0 3.1352439572770514 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACAG 1385 0.0 16.300356 5 GTACTAG 540 3.6379788E-12 15.635329 1 GCGTAGA 195 0.0033182134 15.2815895 1 CATGGGG 4220 0.0 15.269111 4 GAGTACT 3140 0.0 14.729719 12-13 TATACTG 1225 0.0 14.589927 5 TAGACTC 760 0.0 14.2337675 5 CTAGTAC 610 5.456968E-12 13.877537 3 GTACATG 14145 0.0 13.833916 1 AGTACTT 3770 0.0 13.825139 12-13 TCTGTCG 510 1.0550139E-9 13.811056 8 GTATCAA 11775 0.0 13.454936 1 CTGTCGC 455 4.8950824E-8 13.416177 9 GTACTGA 970 0.0 13.312312 1 TACATGG 14160 0.0 13.283577 2 TAGGACC 645 1.4551915E-11 13.125578 4 ACTAGAC 795 0.0 13.014433 3 TATACGT 255 0.0018754528 12.911107 4 ACATGGG 13780 0.0 12.866543 3 GTATAGA 1010 0.0 12.785092 1 >>END_MODULE