FastQCFastQC Report
Wed 25 May 2016
SRR1295153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732461
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT1501.9048457E-830.1945636
GTACTAG6000.020.8699991
GTACATG94650.017.1325821
TATTACG3003.9103266E-516.6937032
TACATGG97100.016.1822322
TTTAGCG2400.002830515615.6503472
CATGGGG37250.015.3436764
GTATAGG7351.8189894E-1215.333061
GTATATG14300.015.3241241
ACATGGG96800.015.1396383
TATACTG13350.015.0827695
CCTTACG2500.00371476815.0243322
GTATTAG9400.014.6534031
GTATATA17150.014.6029151
TAGACTG11800.014.5043835
GTATAAG10050.014.3286551
GTCTAAG7953.6379788E-1214.1758491
TCTATAC10100.014.0058573
CTTACAC11400.013.8977423
GTGTTCG4001.0333042E-613.8526579
Produced by FastQC (version 0.11.5)