##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295153_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2732461 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.968123973224138 27.0 27.0 33.0 6.0 33.0 2 22.04107871987926 27.0 2.0 33.0 2.0 33.0 3 24.321551158461183 27.0 15.0 33.0 6.0 33.0 4 24.257545487382984 33.0 2.0 37.0 2.0 37.0 5 26.28025615004203 33.0 15.0 37.0 2.0 37.0 6 27.200546686668172 33.0 15.0 37.0 2.0 37.0 7 26.894054480558 33.0 15.0 37.0 6.0 37.0 8 27.64177238028283 33.0 15.0 37.0 6.0 37.0 9 28.63263043827524 33.0 22.0 37.0 15.0 37.0 10-11 28.18445606359981 33.0 18.5 37.0 6.0 37.0 12-13 26.986790479351765 33.0 15.0 37.0 4.0 37.0 14-15 28.736420025757 33.0 22.0 37.0 4.0 40.0 16-17 29.13780635844391 33.0 22.0 37.0 6.0 40.0 18-19 29.322181542572793 33.0 22.0 38.5 6.0 40.0 20-21 28.800551407687063 33.0 22.0 38.5 6.0 40.0 22-23 29.575314341174494 33.0 24.5 37.0 6.0 40.0 24-25 29.702469678432738 33.0 24.5 37.0 6.0 40.0 26-27 28.249737690675182 33.0 18.5 37.0 4.0 40.0 28-29 28.975487664782772 33.0 22.0 37.0 6.0 40.0 30-31 29.697687359490217 33.0 24.5 37.0 10.5 40.0 32-33 28.43561481755824 33.0 22.0 37.0 4.0 40.0 34-35 28.07314907696761 33.0 18.5 37.0 6.0 40.0 36-37 28.356118714960616 33.0 22.0 37.0 6.0 40.0 38-39 28.51270155365438 33.0 22.0 37.0 6.0 40.0 40-41 27.551737609429743 33.0 15.0 37.0 6.0 40.0 42-43 28.16890780874823 33.0 22.0 37.0 6.0 40.0 44-45 28.062052669736183 33.0 22.0 37.0 6.0 40.0 46-47 27.77981442370083 33.0 22.0 37.0 6.0 40.0 48-49 27.711908239495457 33.0 18.5 37.0 6.0 40.0 50-51 27.60099686692692 33.0 22.0 37.0 6.0 40.0 52-53 27.824632629706336 33.0 22.0 37.0 6.0 40.0 54-55 27.789399006975763 33.0 22.0 37.0 6.0 40.0 56-57 27.775401002978633 33.0 22.0 37.0 6.0 40.0 58-59 27.678965774808866 33.0 22.0 37.0 6.0 40.0 60-61 27.64265876072888 33.0 22.0 37.0 6.0 40.0 62-63 27.652839692862955 33.0 22.0 37.0 6.0 40.0 64-65 27.154890591302127 33.0 22.0 37.0 6.0 40.0 66-67 27.361439925400582 33.0 22.0 37.0 6.0 38.5 68-69 26.929397162484662 33.0 22.0 37.0 6.0 37.0 70-71 27.09552268083607 33.0 22.0 37.0 6.0 37.0 72-73 27.161664887440296 27.0 22.0 37.0 10.5 37.0 74-75 27.295773114419568 27.0 22.0 37.0 6.0 37.0 76-77 27.05445658693756 27.0 22.0 35.0 6.0 37.0 78-79 26.618697577019397 27.0 22.0 33.0 6.0 37.0 80-81 26.42858891673111 27.0 22.0 33.0 6.0 37.0 82-83 26.559846050867698 27.0 22.0 33.0 10.5 37.0 84-85 26.505744638258335 27.0 22.0 33.0 6.0 37.0 86-87 26.328626465299962 27.0 22.0 33.0 10.5 37.0 88-89 26.280617179897536 27.0 22.0 33.0 10.5 37.0 90-91 26.43170460621396 27.0 22.0 33.0 15.0 37.0 92-93 26.357688728219728 27.0 22.0 33.0 10.5 37.0 94-95 26.220338551950057 27.0 22.0 33.0 10.5 37.0 96-97 26.300641985375087 27.0 22.0 33.0 10.5 37.0 98-99 26.37497515975525 27.0 22.0 33.0 15.0 37.0 100 26.06545893976163 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.194559161881134 1101 2 11.168929233251877 1101 3 8.33413645822387 1101 4 12.585548221600192 1101 5 10.675006815109317 1101 6 9.901358943067379 1101 7 9.439035383125887 1101 8 8.887645194135878 1101 9 7.311355837358587 1101 10-11 7.63594723425846 1101 12-13 9.068409788188436 1101 14-15 7.8667866152190875 1101 16-17 8.052843751459303 1101 18-19 7.950985835666014 1101 20-21 7.205415919665846 1101 22-23 7.278250353750376 1101 24-25 7.2421578418033725 1101 26-27 8.32444385605016 1101 28-29 8.015274689952793 1101 30-31 5.425937260889324 1101 32-33 6.872306404736474 1101 34-35 8.62917821653863 1101 36-37 8.056319653889965 1101 38-39 8.131435211484867 1101 40-41 8.976626442449621 1101 42-43 8.507277981481774 1101 44-45 8.409338772791195 1101 46-47 7.144903555810394 1101 48-49 5.348985091476262 1101 50-51 6.766664815598677 1101 52-53 5.816486401950954 1101 54-55 3.948144083593384 1101 56-57 6.142337322614903 1101 58-59 6.213171037649289 1101 60-61 6.653429524014889 1101 62-63 6.155129753379079 1101 64-65 6.622934397278371 1101 66-67 5.797215159701182 1101 68-69 6.65327220672398 1101 70-71 6.2196348805410295 1101 72-73 5.414727580498607 1101 74-75 4.85976257419761 1101 76-77 5.084616980717893 1101 78-79 5.027221122172492 1101 80-81 4.841775209818909 1101 82-83 4.484130907711371 1101 84-85 4.231543333326297 1101 86-87 3.9728860824064647 1101 88-89 3.943124307781815 1101 90-91 3.8092718486223553 1101 92-93 3.7101788610110127 1101 94-95 3.5626685844297086 1101 96-97 3.757805387056049 1101 98-99 3.4823349389198874 1101 100 3.757460825522333 1102 1 6.749860508556132 1102 2 10.455781052393679 1102 3 8.091715120230006 1102 4 12.402692848500322 1102 5 10.462002433432051 1102 6 9.415470772639921 1102 7 6.706658028022158 1102 8 7.674184198520905 1102 9 6.408595126127178 1102 10-11 7.373420915393204 1102 12-13 8.987997662663854 1102 14-15 6.114160689203754 1102 16-17 7.135702688914517 1102 18-19 7.3996186200627285 1102 20-21 6.987809605671771 1102 22-23 6.465917009023865 1102 24-25 6.917794215174286 1102 26-27 8.363000336352933 1102 28-29 7.471424260580921 1102 30-31 4.394140701505069 1102 32-33 4.398797900716428 1102 34-35 5.110983586722476 1102 36-37 5.175118258192384 1102 38-39 4.593569373581115 1102 40-41 6.231912638373593 1102 42-43 6.822545928981956 1102 44-45 6.482293806820184 1102 46-47 5.591745259114536 1102 48-49 3.6867732365917156 1102 50-51 4.929406812321265 1102 52-53 5.729602237703546 1102 54-55 5.358864556031506 1102 56-57 6.308620086599486 1102 58-59 6.038692407528149 1102 60-61 6.039696860585167 1102 62-63 4.239603272101117 1102 64-65 6.031150962370376 1102 66-67 3.977903207560228 1102 68-69 6.116918799785861 1102 70-71 5.3512540049417545 1102 72-73 4.940079692795933 1102 74-75 4.212659821451652 1102 76-77 3.353303718639001 1102 78-79 4.2851546810352055 1102 80-81 4.350749213866916 1102 82-83 3.7271640868701503 1102 84-85 2.9693403310694855 1102 86-87 3.1378743998060656 1102 88-89 3.3047877628438087 1102 90-91 3.255397469053225 1102 92-93 3.116028192214859 1102 94-95 3.0788802764235115 1102 96-97 3.214211901992524 1102 98-99 3.092495912138883 1102 100 3.151316832975869 1103 1 6.665875400256169 1103 2 11.082197518504863 1103 3 7.900657668672956 1103 4 11.774582882572496 1103 5 10.311324207807733 1103 6 9.11616208192926 1103 7 8.235141834634053 1103 8 7.949520518532168 1103 9 7.1042422727346555 1103 10-11 7.3841914582651285 1103 12-13 8.634673531407064 1103 14-15 8.445822202343276 1103 16-17 7.952533124008614 1103 18-19 7.223237382706227 1103 20-21 8.322252672604279 1103 22-23 6.910412975247969 1103 24-25 5.191567386314691 1103 26-27 6.1333095085181775 1103 28-29 7.0293781726878315 1103 30-31 6.183104038100719 1103 32-33 7.994913053968343 1103 34-35 7.064699028847141 1103 36-37 4.430636908638139 1103 38-39 6.537044130911003 1103 40-41 6.8792104061971315 1103 42-43 7.632959888857901 1103 44-45 5.672583784550632 1103 46-47 6.355613746727855 1103 48-49 5.587531891845945 1103 50-51 6.375067422050414 1103 52-53 6.048722906130081 1103 54-55 5.098040433881678 1103 56-57 6.027104907119526 1103 58-59 5.616307804846578 1103 60-61 5.147176861702292 1103 62-63 2.9037287429309195 1103 64-65 5.140997710457306 1103 66-67 4.268848309594603 1103 68-69 5.28785821907756 1103 70-71 4.009876291978543 1103 72-73 4.594689499048407 1103 74-75 3.4542328938726214 1103 76-77 3.7511193688880446 1103 78-79 3.1578110809205775 1103 80-81 4.232240214369622 1103 82-83 3.534217351047257 1103 84-85 3.402851088209953 1103 86-87 2.973063068684308 1103 88-89 3.0578013164279447 1103 90-91 2.7906716067455974 1103 92-93 2.4631998741852605 1103 94-95 2.838892649745709 1103 96-97 3.0341251946319154 1103 98-99 2.7425409156966687 1103 100 3.01454724147764 1104 1 6.545673631667306 1104 2 9.779928471074523 1104 3 7.433650569407792 1104 4 12.207211195845964 1104 5 9.740844469586062 1104 6 8.202518358608117 1104 7 4.115950041248713 1104 8 6.499317278006348 1104 9 6.64184799743715 1104 10-11 7.161463053278659 1104 12-13 7.564438927301492 1104 14-15 7.320814559593696 1104 16-17 7.098175624489823 1104 18-19 6.836318939336163 1104 20-21 7.529362694230436 1104 22-23 6.4206570903307885 1104 24-25 5.94640269921452 1104 26-27 8.30003014620656 1104 28-29 7.21737452681322 1104 30-31 6.322925027921709 1104 32-33 7.692987647204223 1104 34-35 8.024377580461174 1104 36-37 6.778894927863895 1104 38-39 7.348543355313449 1104 40-41 7.263134590121311 1104 42-43 7.30133588949197 1104 44-45 6.715395070910304 1104 46-47 6.699943958800006 1104 48-49 5.5315558278053665 1104 50-51 5.717065746306805 1104 52-53 6.021229388313984 1104 54-55 4.916116385828598 1104 56-57 6.132017779774333 1104 58-59 4.025348894532826 1104 60-61 4.500575903811008 1104 62-63 4.0433929148531575 1104 64-65 2.854084362253637 1104 66-67 3.20218711551405 1104 68-69 4.326027808860051 1104 70-71 2.751231493011165 1104 72-73 2.567924023895838 1104 74-75 3.2803812877629497 1104 76-77 3.9031115789447703 1104 78-79 4.480369080897933 1104 80-81 3.287844897393658 1104 82-83 3.065405996752034 1104 84-85 3.0336898658229217 1104 86-87 0.8545025098011685 1104 88-89 0.42214873883988346 1104 90-91 2.305272089088014 1104 92-93 2.415555539444153 1104 94-95 2.610613910176646 1104 96-97 2.991342780881755 1104 98-99 2.5955159267361942 1104 100 1.1450537010163586 1105 1 5.766085472079144 1105 2 -2.836772125626073 1105 3 0.3953447311019538 1105 4 8.311409160043933 1105 5 8.621008269749861 1105 6 8.120494256584017 1105 7 5.196864982163653 1105 8 5.907687466376537 1105 9 4.967871163460316 1105 10-11 4.918532200347805 1105 12-13 5.081749094611663 1105 14-15 5.931525920305056 1105 16-17 6.0246550909692935 1105 18-19 6.103157596174821 1105 20-21 6.95853667340441 1105 22-23 2.048289007962712 1105 24-25 4.003936336748158 1105 26-27 6.814430520606926 1105 28-29 5.9438083632470615 1105 30-31 5.316673711670386 1105 32-33 7.017858362074943 1105 34-35 6.368024474108072 1105 36-37 6.346289335258305 1105 38-39 4.229881836651934 1105 40-41 5.572031279018002 1105 42-43 5.631243459399538 1105 44-45 6.167831013346248 1105 46-47 5.415345854201899 1105 48-49 4.888449474699012 1105 50-51 6.006958106199161 1105 52-53 5.592875579960179 1105 54-55 5.3143742540864665 1105 56-57 5.232567680324237 1105 58-59 1.8845345737185042 1105 60-61 4.340633233868338 1105 62-63 -0.9617925303322892 1105 64-65 -0.4283796602103891 1105 66-67 0.9255766089035475 1105 68-69 2.846828089660331 1105 70-71 2.996032007811678 1105 72-73 2.6929550289268427 1105 74-75 2.6420380094196716 1105 76-77 1.653445029278224 1105 78-79 3.246420326949181 1105 80-81 1.6983128978616584 1105 82-83 1.0651638065098403 1105 84-85 1.3835354256684802 1105 86-87 -0.2341028488041914 1105 88-89 1.630703787394932 1105 90-91 1.4222909261068502 1105 92-93 1.7517296356182506 1105 94-95 1.1302453598080966 1105 96-97 2.0203728499118263 1105 98-99 1.9043236955439653 1105 100 2.021342457305117 1106 1 6.133704519698192 1106 2 10.418077666514488 1106 3 6.813035007463501 1106 4 11.843393517360475 1106 5 9.879337699882573 1106 6 9.53092091936616 1106 7 6.644965262671182 1106 8 7.375770938843097 1106 9 7.008415743987644 1106 10-11 5.906922550291217 1106 12-13 8.015569487621004 1106 14-15 7.929499244076485 1106 16-17 7.783595512066743 1106 18-19 7.2447315758333595 1106 20-21 8.354532251022071 1106 22-23 6.268325163926388 1106 24-25 5.229211222920373 1106 26-27 7.6886090369424736 1106 28-29 6.195599316038869 1106 30-31 5.5037765656757465 1106 32-33 7.415062101677304 1106 34-35 6.947279989946846 1106 36-37 6.122757554987956 1106 38-39 7.137511193202773 1106 40-41 6.81498448916707 1106 42-43 6.26658912609982 1106 44-45 4.564702700761508 1106 46-47 6.133772397460195 1106 48-49 5.922035526266082 1106 50-51 6.822781494774919 1106 52-53 5.842419633835551 1106 54-55 5.442387580995394 1106 56-57 4.950973979230966 1106 58-59 5.065059208703886 1106 60-61 4.994038953831435 1106 62-63 5.101213120904596 1106 64-65 5.308343444122727 1106 66-67 3.270213088328635 1106 68-69 3.5250155105224756 1106 70-71 3.0900012080103885 1106 72-73 2.8818293010452933 1106 74-75 2.9655118953266886 1106 76-77 3.639324033793983 1106 78-79 4.267229129880501 1106 80-81 3.661939027268634 1106 82-83 2.3583025400799826 1106 84-85 2.9374374684057294 1106 86-87 3.4886239383574527 1106 88-89 3.431272870310867 1106 90-91 3.2654606238235715 1106 92-93 3.0956656305982477 1106 94-95 1.6939561270391401 1106 96-97 1.6534729623389453 1106 98-99 2.0829970555460555 1106 100 2.9065858190627125 1107 1 6.181284049435398 1107 2 10.396480734929693 1107 3 6.167471601465341 1107 4 11.306228006481035 1107 5 9.312002144478175 1107 6 8.023016754832657 1107 7 3.8193984376431658 1107 8 6.24583488603848 1107 9 5.652247050367325 1107 10-11 4.574103592993886 1107 12-13 6.145713501573994 1107 14-15 6.14401985790273 1107 16-17 6.96197820354136 1107 18-19 6.21747546364622 1107 20-21 5.7333866850801485 1107 22-23 5.806168379628385 1107 24-25 4.564212198507423 1107 26-27 7.18056365096205 1107 28-29 6.481478290138984 1107 30-31 5.231005051146962 1107 32-33 5.061203460959458 1107 34-35 6.699824019652429 1107 36-37 6.537516859463011 1107 38-39 6.582050829484825 1107 40-41 7.682108428700964 1107 42-43 6.2456967205229255 1107 44-45 4.182011017298038 1107 46-47 3.4691670537492385 1107 48-49 4.093123407267132 1107 50-51 4.886010395541906 1107 52-53 4.019582617735679 1107 54-55 5.2934936789216565 1107 56-57 5.294319759503704 1107 58-59 3.203822802778518 1107 60-61 2.9645775226362794 1107 62-63 0.5561108215025996 1107 64-65 2.1513514385663512 1107 66-67 4.148069827994274 1107 68-69 5.057684460122513 1107 70-71 4.29608356068481 1107 72-73 2.5614780031863305 1107 74-75 2.7478841411579182 1107 76-77 3.087370681772338 1107 78-79 3.903606384321961 1107 80-81 2.346224557433068 1107 82-83 1.6530800465630122 1107 84-85 2.440172364110399 1107 86-87 2.4321386413792077 1107 88-89 2.6354641653295836 1107 90-91 1.5744694840634175 1107 92-93 2.3853307924144254 1107 94-95 2.440042516316872 1107 96-97 2.4259817396389742 1107 98-99 1.9825888156244353 1107 100 0.2771417962787268 1108 1 4.418047610235973 1108 2 -3.1927854927898824 1108 3 0.059972286437474764 1108 4 6.702515222123445 1108 5 7.206167594201222 1108 6 6.9939002606094895 1108 7 4.401994778620885 1108 8 5.091680965502782 1108 9 4.970054113253887 1108 10-11 3.487056110066405 1108 12-13 2.5870622917390165 1108 14-15 4.152141956186579 1108 16-17 4.733360279585984 1108 18-19 3.9554128925009167 1108 20-21 4.813180407251686 1108 22-23 4.171634497281648 1108 24-25 2.936150125908849 1108 26-27 4.69969102706213 1108 28-29 6.123620188589857 1108 30-31 4.573343767278502 1108 32-33 4.5761749275154 1108 34-35 3.0579398684482406 1108 36-37 1.4813596778950213 1108 38-39 2.6026220020912127 1108 40-41 5.497335900650057 1108 42-43 3.259018663546424 1108 44-45 3.692852407880917 1108 46-47 4.745672297669934 1108 48-49 5.014579053085228 1108 50-51 2.2606413109000663 1108 52-53 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24-25 -25.147665394853572 2212 26-27 -17.215040223702523 2212 28-29 -18.086283802651558 2212 30-31 -6.303108441660147 2212 32-33 -17.202957166482523 2212 34-35 -13.684740502850454 2212 36-37 -17.565246763051984 2212 38-39 -23.609512102742006 2212 40-41 -10.087921241579679 2212 42-43 -13.795206757050677 2212 44-45 -17.114980046561584 2212 46-47 -17.054779826568943 2212 48-49 -17.68506120816651 2212 50-51 -8.999574500978607 2212 52-53 -2.1034629202492923 2212 54-55 -23.49376427405206 2212 56-57 -23.359207211450656 2212 58-59 -12.696010042955143 2212 60-61 -6.392547886086973 2212 62-63 -3.291849784905672 2212 64-65 -17.335786446972016 2212 66-67 -17.57994356984244 2212 68-69 -14.665647821525257 2212 70-71 -9.703350759958187 2212 72-73 -17.474042597103043 2212 74-75 -6.322071751786865 2212 76-77 -10.944565025183444 2212 78-79 -4.883906443055007 2212 80-81 -2.600117488955828 2212 82-83 -3.022196897302475 2212 84-85 -5.701458290292976 2212 86-87 -5.149131476090883 2212 88-89 -3.5106256358699746 2212 90-91 -4.322169344805033 2212 92-93 -5.715857293581582 2212 94-95 -4.078338636840414 2212 96-97 -5.27262533469926 2212 98-99 -3.9414296908545836 2212 100 -3.9805455471635334 2213 1 -21.066295480301807 2213 2 -17.0440448528988 2213 3 -19.82889769314047 2213 4 -18.796123307488536 2213 5 -21.078039349297757 2213 6 -21.85838020229044 2213 7 -10.58659383847101 2213 8 -10.398252496561648 2213 9 -4.3786527921577765 2213 10-11 -17.537815058077605 2213 12-13 -16.192193451888613 2213 14-15 -23.50570351692438 2213 16-17 -17.939009761052578 2213 18-19 -18.613259595277892 2213 20-21 -17.650057322889406 2213 22-23 -19.751208158577086 2213 24-25 -7.865249764302948 2213 26-27 -23.21734437116796 2213 28-29 -24.12476306532437 2213 30-31 -19.42671306141443 2213 32-33 -23.29235808814151 2213 34-35 -17.839936703258083 2213 36-37 -23.647043998075027 2213 38-39 -11.55467196064609 2213 40-41 -17.061805387571773 2213 42-43 -23.202349614193533 2213 44-45 -17.151830240142715 2213 46-47 -16.99667336104112 2213 48-49 -6.503215884808267 2213 50-51 -23.333215053973998 2213 52-53 -17.849692180875792 2213 54-55 -17.678710988084035 2213 56-57 -17.501303126193108 2213 58-59 -17.995397377101543 2213 60-61 -19.36423347431986 2213 62-63 -17.59777897432051 2213 64-65 -17.608597302248633 2213 66-67 -8.893667776077962 2213 68-69 -22.698366715534473 2213 70-71 -17.454411772800842 2213 72-73 -9.625795672710183 2213 74-75 -4.755337829661318 2213 76-77 -3.467553909615919 2213 78-79 -4.987017656026989 2213 80-81 -3.5175258755614855 2213 82-83 -3.4405613209381976 2213 84-85 -3.2498130553354194 2213 86-87 -3.3489718637442536 2213 88-89 -3.078116693485942 2213 90-91 -3.128347775038069 2213 92-93 -3.844458802941414 2213 94-95 -3.982028751515749 2213 96-97 -3.690690178238235 2213 98-99 -3.9498482189831883 2213 100 -3.421045338439697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 747.0 3 823.0 4 4679.0 5 14827.0 6 21441.0 7 27685.0 8 26376.0 9 26347.0 10 30579.0 11 36180.0 12 44602.0 13 58440.0 14 47522.0 15 51481.0 16 57656.0 17 60723.0 18 60400.0 19 58337.0 20 57348.0 21 56089.0 22 57612.0 23 62626.0 24 68511.0 25 74479.0 26 81397.0 27 87900.0 28 93642.0 29 98406.0 30 102080.0 31 111221.0 32 125176.0 33 149951.0 34 181131.0 35 210853.0 36 230383.0 37 196218.0 38 58487.0 39 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.77837292199993 20.79609793710739 13.828591265230935 24.59693787566174 2 14.802142013345994 21.03271541186128 40.92709015131336 23.238052423479367 3 17.808552460138188 26.955181279028956 29.837078216684283 25.399188044148573 4 12.67881991468661 17.801394852886073 38.784580503308845 30.735204729118472 5 13.064590755858113 37.70448211999394 36.16366457743931 13.06726254670863 6 30.14566100569653 38.816879262499604 18.29044966203643 12.74701006976743 7 26.01023333664444 32.1466561086537 23.693556488653687 18.149554066048175 8 24.315097199405063 36.509576187350454 21.670363079978184 17.504963533266302 9 24.495769410881017 17.49381131704991 22.18552257262206 35.82489669944701 10-11 23.23647847776144 27.572190968436423 29.103432991465507 20.087897562336625 12-13 23.993155080566115 25.19898141275877 29.054250144180482 21.753613362494633 14-15 21.40004692988372 26.769488073185077 26.516800978184783 25.31366401874641 16-17 21.02635568138981 30.330308231538588 27.314784366252535 21.328551720819064 18-19 21.038180767402455 29.40003905456396 28.827436028426987 20.734344149606596 20-21 22.320341940644948 28.698299979950605 27.979153449400187 21.00220463000426 22-23 21.508262623170022 28.66067634948108 28.30762670032429 21.523434327024614 24-25 21.69983161915002 28.616128034020825 27.90310116796831 21.78093917886084 26-27 21.65960853506414 28.457736014352637 28.04005857100097 21.842596879582253 28-29 21.481843342224487 28.396884143006577 28.475765851713007 21.645506663055926 30-31 21.98704452602348 28.767668147074808 27.87453056505261 21.3707567618491 32-33 21.23480558128236 28.606045883121023 28.394374180748926 21.764774354847688 34-35 21.304805316122373 28.219505741525875 28.248013083955716 22.22767585839604 36-37 21.904898621046087 28.661635791052475 27.85353476690852 21.579930820992914 38-39 21.703581505620612 28.391973388863345 28.04814430278438 21.856300802731663 40-41 21.73969553834591 28.458817270533643 27.815043218313228 21.986443972807216 42-43 21.601551656698714 28.512184716228834 28.211568974436624 21.67469465263583 44-45 21.961060316679905 28.14085684479911 28.116332904706077 21.781749933814908 46-47 21.772677091800215 28.471971381822637 28.266046360915105 21.489305165462046 48-49 21.64954943732526 28.308898873540695 28.313319062437753 21.728232626696293 50-51 21.88341281601199 28.336757592271923 28.10495148039902 21.67487811131707 52-53 21.439167479875493 28.31962771107108 28.13988606076502 22.101318748288403 54-55 21.83379085165153 28.417378555856427 27.901011507420026 21.84781908507202 56-57 21.89961549848773 28.300878722379068 27.864845880105964 21.93465989902723 58-59 21.65139582806227 27.830865206112502 28.324846288206945 22.192892677618286 60-61 21.98003796098878 28.328363895964713 27.903840091876873 21.787758051169632 62-63 21.577699959101352 28.38303847255182 28.160338605240593 21.878922963106238 64-65 21.581207255747888 27.987938591203843 28.204161313337973 22.226692839710296 66-67 21.730155287528355 28.15114272722176 27.951169946473208 22.167532038776674 68-69 22.213353809656418 27.984554203025542 27.898193989857383 21.903897997460657 70-71 22.102770294099297 28.162806307489053 27.712761455682855 22.021661942728795 72-73 21.89966626744007 28.182303787210362 28.080092832298366 21.837937113051208 74-75 22.143480308046005 27.845316440591272 27.868332518411478 22.14287073295125 76-77 22.02566565724592 28.21297326439999 27.89297237509692 21.868388703257168 78-79 22.127932551661537 28.041581800677985 28.02264842322273 21.807837224437748 80-81 22.15203754139367 27.91301602203135 27.897256831468404 22.03768960510658 82-83 21.701294710136835 28.15572329435391 28.170803174342424 21.972178821166835 84-85 21.938184572965962 27.853633272935834 27.769659236439132 22.43852291765907 86-87 22.08519058100566 28.053841000233128 27.861564846226877 21.99940357253433 88-89 21.957320448477 27.663327251342196 28.150561977822992 22.22879032235781 90-91 22.168928691825712 27.729772638317712 27.89497218917742 22.206326480679152 92-93 21.790548293840757 28.02258550991999 28.07656336578002 22.110302830459226 94-95 21.757212939241096 27.640629415885638 28.062676462817493 22.539481182055773 96-97 21.987508721044247 27.719138275206234 28.057631716999374 22.23572128675014 98-99 21.992980533255114 27.607248238466937 28.02609910192387 22.37367212635408 100 22.036817464442105 27.480525762154446 27.87497234242903 22.60768443097442 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 319.0 1 388.0 2 383.5 3 312.0 4 358.0 5 494.5 6 759.5 7 1122.0 8 1628.5 9 2384.0 10 3206.0 11 4108.0 12 5096.0 13 6067.5 14 7040.5 15 7934.5 16 8882.0 17 9914.0 18 10773.0 19 11520.5 20 12501.0 21 13663.5 22 14837.5 23 16412.0 24 18582.5 25 21341.0 26 24925.0 27 29834.0 28 35952.5 29 42967.0 30 50570.5 31 58432.0 32 66216.5 33 73859.0 34 80733.5 35 86946.0 36 92330.0 37 96674.5 38 100418.0 39 102863.5 40 103344.5 41 102430.5 42 101084.5 43 98686.0 44 95202.5 45 91328.0 46 87639.0 47 84041.5 48 81010.5 49 78856.0 50 77207.5 51 73582.0 52 66559.0 53 59581.0 54 54445.0 55 50132.5 56 45721.5 57 41248.0 58 37018.0 59 32749.5 60 28242.0 61 23830.0 62 19912.5 63 16339.5 64 13260.5 65 10677.0 66 8395.5 67 6541.0 68 5100.0 69 3976.5 70 3054.0 71 2298.5 72 1725.5 73 1313.5 74 989.5 75 721.0 76 504.5 77 348.5 78 242.0 79 158.0 80 116.0 81 79.5 82 45.5 83 35.0 84 28.5 85 19.0 86 13.0 87 6.5 88 5.0 89 5.5 90 4.5 91 3.0 92 2.0 93 2.0 94 0.5 95 0.5 96 0.5 97 0.5 98 1.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.491978476545501 2 26.081945908834562 3 6.888808294061653 4 25.28167831123665 5 13.70530082588553 6 10.80944979635574 7 8.998554782666615 8 9.623193158109117 9 6.063361929044916 10-11 7.5264020236702365 12-13 11.096736604840837 14-15 8.292707562889278 16-17 8.742338865952707 18-19 7.229599983311748 20-21 8.915333100820103 22-23 6.286384325338952 24-25 5.921420287425877 26-27 10.561523110485382 28-29 6.955140439332895 30-31 4.6308254719829485 32-33 9.610329296557206 34-35 7.953251665806025 36-37 7.839508047873327 38-39 6.6258219238993705 40-41 9.33367392983834 42-43 7.185207766917808 44-45 7.104401490085312 46-47 6.483605804437831 48-49 4.371535403433023 50-51 7.29413887334531 52-53 5.118133433560442 54-55 5.691389556886631 56-57 5.438174597917408 58-59 3.955006128175297 60-61 4.624567377173911 62-63 3.314393142299195 64-65 5.1318756242083605 66-67 3.0595313162749624 68-69 5.77428186532214 70-71 2.921780036384783 72-73 1.8215813510238572 74-75 0.9389886991982686 76-77 1.2339974843190809 78-79 1.9033940466121932 80-81 2.4647927271423087 82-83 0.8619885151151289 84-85 1.353541733989982 86-87 0.4733827857012414 88-89 0.6647304389705837 90-91 0.23290359862409749 92-93 0.3338382505733842 94-95 0.61367756026527 96-97 0.6238515389606658 98-99 0.49748194027288956 100 0.42770967270896093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2732461.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.37647218550877 #Duplication Level Percentage of deduplicated Percentage of total 1 97.09458308296209 92.60538802775706 2 2.2871683480480285 4.362840966623577 3 0.3539523117984361 1.0127616846372023 4 0.11553532848936605 0.44077408176438565 5 0.055362284717650354 0.26401297042495986 6 0.02758124895617396 0.1578361334346071 7 0.016767921846659244 0.11194856621316762 8 0.008578301417320959 0.06545345012224184 9 0.009903953800912798 0.0850143756797391 >10 0.02874849220844656 0.41830189038390764 >50 5.437329323126477E-4 0.036143118114514684 >100 0.001082310139823714 0.22972839881837703 >500 1.156096096778846E-4 0.07608241338055105 >1k 7.707307311809255E-5 0.13371392264574478 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.6597045666891494E-5 0.0 0.0 0.0 3 0.0 3.6597045666891494E-5 0.0 0.0 0.0 4 0.0 3.6597045666891494E-5 0.0 0.0 0.0 5 0.0 3.6597045666891494E-5 0.0 0.0 0.0 6 0.0 3.6597045666891494E-5 0.0 0.0 0.0 7 0.0 3.6597045666891494E-5 0.0 0.0 0.0 8 0.0 3.6597045666891494E-5 0.0 0.0 0.0 9 0.0 3.6597045666891494E-5 0.0 7.319409133378299E-5 0.0 10-11 0.0 5.489556850033724E-5 0.0 7.319409133378299E-5 0.0 12-13 0.0 9.149261416722874E-5 0.0 9.149261416722874E-5 0.0 14-15 0.0 1.0979113700067448E-4 0.0 1.4638818266756598E-4 0.0 16-17 0.0 1.0979113700067448E-4 0.0 1.4638818266756598E-4 0.0 18-19 3.6597045666891494E-5 1.0979113700067448E-4 0.0 1.646867055010117E-4 0.0 20-21 3.6597045666891494E-5 1.0979113700067448E-4 0.0 2.0128375116790323E-4 0.0 22-23 3.6597045666891494E-5 1.0979113700067448E-4 0.0 3.110748881685777E-4 0.0 24-25 3.6597045666891494E-5 1.0979113700067448E-4 0.0 4.5746307083614367E-4 0.0 26-27 5.489556850033724E-5 1.0979113700067448E-4 0.0 5.489556850033724E-4 0.0 28-29 7.319409133378299E-5 1.0979113700067448E-4 0.0 8.600305731719501E-4 0.0 30-31 7.319409133378299E-5 1.0979113700067448E-4 0.0 0.002634987288016188 3.6597045666891494E-5 32-33 7.319409133378299E-5 1.0979113700067448E-4 0.0 0.00786836481838167 3.6597045666891494E-5 34-35 7.319409133378299E-5 1.0979113700067448E-4 0.0 0.014858400540757946 3.6597045666891494E-5 36-37 7.319409133378299E-5 1.829852283344575E-4 0.0 0.025654529012490938 3.6597045666891494E-5 38-39 7.319409133378299E-5 2.561793196682405E-4 0.0 0.04250746854209447 3.6597045666891494E-5 40-41 7.319409133378299E-5 2.561793196682405E-4 0.0 0.064081426962727 3.6597045666891494E-5 42-43 1.0979113700067448E-4 2.561793196682405E-4 0.0 0.0866801026620325 3.6597045666891494E-5 44-45 1.0979113700067448E-4 2.561793196682405E-4 0.0 0.11431087214053559 3.6597045666891494E-5 46-47 1.0979113700067448E-4 2.561793196682405E-4 0.0 0.1481631393824102 3.6597045666891494E-5 48-49 1.0979113700067448E-4 2.744778425016862E-4 0.0 0.18190561548728418 3.6597045666891494E-5 50-51 1.0979113700067448E-4 2.9277636533513196E-4 0.0 0.21850266115417566 3.6597045666891494E-5 52-53 1.0979113700067448E-4 3.293734110020235E-4 0.0 0.2594364567325938 3.6597045666891494E-5 54-55 1.0979113700067448E-4 3.8426897950236073E-4 0.0 0.30445082290287034 3.6597045666891494E-5 56-57 1.0979113700067448E-4 4.0256750233580647E-4 0.0 0.35993194413387786 3.6597045666891494E-5 58-59 1.0979113700067448E-4 4.0256750233580647E-4 0.0 0.4184689186780708 3.6597045666891494E-5 60-61 1.0979113700067448E-4 4.3916454800269793E-4 0.0 0.4817452106361262 3.6597045666891494E-5 62-63 1.0979113700067448E-4 4.3916454800269793E-4 0.0 0.5472905194255289 3.6597045666891494E-5 64-65 1.646867055010117E-4 4.3916454800269793E-4 0.0 0.6213080442868169 3.6597045666891494E-5 66-67 1.8298522833445747E-4 4.7576159366958945E-4 0.0 0.7108244179880335 3.6597045666891494E-5 68-69 1.8298522833445747E-4 4.7576159366958945E-4 0.0 0.8136987133576655 3.6597045666891494E-5 70-71 1.8298522833445747E-4 4.940601165030352E-4 0.0 0.9320535590443926 3.6597045666891494E-5 72-73 1.8298522833445747E-4 5.12358639336481E-4 0.0 1.0588989193258385 3.6597045666891494E-5 74-75 1.8298522833445747E-4 5.12358639336481E-4 0.0 1.191142343843151 3.6597045666891494E-5 76-77 1.8298522833445747E-4 5.12358639336481E-4 0.0 1.3377135117390515 3.6597045666891494E-5 78-79 1.8298522833445747E-4 5.12358639336481E-4 0.0 1.5069199523799242 3.6597045666891494E-5 80-81 1.8298522833445747E-4 5.12358639336481E-4 0.0 1.6992740244051059 3.6597045666891494E-5 82-83 1.8298522833445747E-4 5.12358639336481E-4 0.0 1.9085725285740582 3.6597045666891494E-5 84-85 1.8298522833445747E-4 5.12358639336481E-4 0.0 2.123250798456044 3.6597045666891494E-5 86-87 1.8298522833445747E-4 5.12358639336481E-4 0.0 2.364718105766194 3.6597045666891494E-5 88 1.8298522833445747E-4 5.12358639336481E-4 0.0 2.569368785135451 3.6597045666891494E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 150 1.9048457E-8 30.194563 6 GTACTAG 600 0.0 20.869999 1 GTACATG 9465 0.0 17.132582 1 TATTACG 300 3.9103266E-5 16.693703 2 TACATGG 9710 0.0 16.182232 2 TTTAGCG 240 0.0028305156 15.650347 2 CATGGGG 3725 0.0 15.343676 4 GTATAGG 735 1.8189894E-12 15.33306 1 GTATATG 1430 0.0 15.324124 1 ACATGGG 9680 0.0 15.139638 3 TATACTG 1335 0.0 15.082769 5 CCTTACG 250 0.003714768 15.024332 2 GTATTAG 940 0.0 14.653403 1 GTATATA 1715 0.0 14.602915 1 TAGACTG 1180 0.0 14.504383 5 GTATAAG 1005 0.0 14.328655 1 GTCTAAG 795 3.6379788E-12 14.175849 1 TCTATAC 1010 0.0 14.005857 3 CTTACAC 1140 0.0 13.897742 3 GTGTTCG 400 1.0333042E-6 13.852657 9 >>END_MODULE