FastQCFastQC Report
Wed 25 May 2016
SRR1295153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732461
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62610.22913410292040765No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42940.15714771409363207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC758.366035E-418.77645522-23
GAGTACT33750.017.45802512-13
GTATAGG7850.017.219111
TAGATCG1650.001565961617.1008135
ATCGACC1952.4889293E-416.8534418
GTATCAA100000.016.5708431
AACGCAG106100.015.7985716
CAACGCA106400.015.7788765
TCAACGC106700.015.7785864
ATCAACG107300.015.5583023
CCTATAC8750.015.0481643
GTACATG125450.015.044831
ATAACCG2206.154218E-414.9632125
TATCAAC109250.014.878932
CATGGGG45600.014.8507064
GTATAGA10150.014.7969361
GTATAAG9500.014.7554021
TACATGG127450.014.4809252
TCGGTAC2601.3262144E-414.4693883
ACATGGG124450.014.20778753
Produced by FastQC (version 0.11.5)