##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295153_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2732461 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.107641060567744 33.0 33.0 33.0 27.0 33.0 2 29.690334098089597 33.0 33.0 33.0 22.0 33.0 3 30.594919012567793 33.0 33.0 33.0 22.0 33.0 4 34.88942349039932 37.0 37.0 37.0 33.0 37.0 5 35.23287468695802 37.0 37.0 37.0 33.0 37.0 6 34.931600853589494 37.0 37.0 37.0 33.0 37.0 7 34.811602434581864 37.0 37.0 37.0 27.0 37.0 8 34.795839355072225 37.0 37.0 37.0 27.0 37.0 9 34.77952585599575 37.0 37.0 37.0 27.0 37.0 10-11 34.746976992535295 37.0 37.0 37.0 27.0 37.0 12-13 34.64350140770536 37.0 37.0 37.0 27.0 37.0 14-15 36.00951998948933 37.0 37.0 40.0 27.0 40.0 16-17 35.99727864368421 37.0 37.0 40.0 27.0 40.0 18-19 36.02741411496815 37.0 37.0 40.0 27.0 40.0 20-21 36.025290571393334 37.0 37.0 40.0 27.0 40.0 22-23 35.98706148047493 37.0 37.0 40.0 27.0 40.0 24-25 35.94853705139799 37.0 37.0 40.0 27.0 40.0 26-27 35.92094397687653 37.0 37.0 40.0 27.0 40.0 28-29 35.86487785187053 37.0 37.0 40.0 27.0 40.0 30-31 35.777178887457126 37.0 37.0 40.0 27.0 40.0 32-33 35.729525325338585 37.0 37.0 40.0 27.0 40.0 34-35 35.61905842388967 37.0 37.0 40.0 27.0 40.0 36-37 35.60796531039235 37.0 37.0 40.0 27.0 40.0 38-39 35.545789125627046 37.0 37.0 40.0 27.0 40.0 40-41 35.48968402476741 37.0 37.0 40.0 27.0 40.0 42-43 35.3879987674115 37.0 35.0 40.0 27.0 40.0 44-45 35.28629027093159 37.0 33.0 40.0 27.0 40.0 46-47 35.18126626509948 37.0 33.0 40.0 27.0 40.0 48-49 35.07738847873767 37.0 33.0 40.0 27.0 40.0 50-51 34.97648731308516 37.0 33.0 40.0 27.0 40.0 52-53 34.88696014325548 37.0 33.0 40.0 22.0 40.0 54-55 34.756040616865164 37.0 33.0 40.0 22.0 40.0 56-57 34.634252785309656 37.0 33.0 40.0 22.0 40.0 58-59 34.50443098730412 37.0 33.0 40.0 22.0 40.0 60-61 34.34034593723387 37.0 33.0 40.0 22.0 40.0 62-63 34.16505267595768 37.0 33.0 40.0 22.0 40.0 64-65 33.97765878451696 37.0 33.0 40.0 22.0 40.0 66-67 33.758593260800424 37.0 33.0 38.5 22.0 40.0 68-69 33.54319878673474 37.0 33.0 37.0 22.0 40.0 70-71 33.313257535972156 37.0 33.0 37.0 22.0 40.0 72-73 33.103550791758785 37.0 33.0 37.0 22.0 40.0 74-75 32.87325912428393 37.0 33.0 37.0 22.0 40.0 76-77 32.64155243203837 37.0 33.0 37.0 22.0 37.0 78-79 32.40491099415509 37.0 33.0 37.0 22.0 37.0 80-81 32.200422256712905 37.0 33.0 37.0 22.0 37.0 82-83 31.996790256109787 33.0 33.0 37.0 22.0 37.0 84-85 31.827949968910808 33.0 33.0 37.0 22.0 37.0 86-87 31.851330906461243 33.0 33.0 37.0 22.0 37.0 88-89 31.86419678085067 33.0 33.0 37.0 22.0 37.0 90-91 31.874315863977564 33.0 33.0 37.0 22.0 37.0 92-93 31.838857535386598 33.0 33.0 37.0 22.0 37.0 94-95 31.866986939612314 33.0 33.0 37.0 22.0 37.0 96-97 31.83156246328859 33.0 33.0 37.0 22.0 37.0 98-99 31.75561792098771 33.0 33.0 37.0 22.0 37.0 100 31.213900582661562 33.0 27.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6276084850038544 1101 2 4.044760464780136 1101 3 2.2289080491199478 1101 4 1.1913442089623345 1101 5 0.4534214504793752 1101 6 0.6276193122908609 1101 7 0.6018315502461888 1101 8 0.6104890117619135 1101 9 0.588300307972986 1101 10-11 0.6537460275400093 1101 12-13 0.6506115280978548 1101 14-15 0.922090531086269 1101 16-17 0.9433841146625852 1101 18-19 0.9281247383140823 1101 20-21 0.9691405640680273 1101 22-23 0.9084666473163665 1101 24-25 0.9971141568752273 1101 26-27 1.0014441147233057 1101 28-29 0.8636670684894554 1101 30-31 0.8578332591353046 1101 32-33 0.9646150625797034 1101 34-35 1.112639149376129 1101 36-37 0.963491668666876 1101 38-39 1.0133862356332557 1101 40-41 1.0288852116434413 1101 42-43 1.1539927870748343 1101 44-45 1.1199101142034422 1101 46-47 1.075964638337851 1101 48-49 1.0938772914728858 1101 50-51 0.9792395018167142 1101 52-53 0.9905343176465848 1101 54-55 1.0335369334312787 1101 56-57 1.0403418133284106 1101 58-59 1.081308423472457 1101 60-61 1.0522197884788227 1101 62-63 1.0893423752921407 1101 64-65 1.0734279234639743 1101 66-67 1.1568554507369129 1101 68-69 0.9787281592710357 1101 70-71 0.9872351583281187 1101 72-73 0.8961768128015137 1101 74-75 0.8879169664594784 1101 76-77 0.927256586773872 1101 78-79 0.9284096622412363 1101 80-81 0.9753573494864511 1101 82-83 1.0049878193120492 1101 84-85 1.0093366348127724 1101 86-87 1.0127000909316415 1101 88-89 0.8687457056315466 1101 90-91 0.9425770043250417 1101 92-93 1.0398946139408345 1101 94-95 1.0223239950136822 1101 96-97 0.9107177908555286 1101 98-99 0.9511253031162532 1101 100 1.0379965560058828 1102 1 0.5422370056786683 1102 2 4.0099462737579366 1102 3 2.2787758602364825 1102 4 1.1287762083523631 1102 5 0.5601098064325782 1102 6 0.5206380099489181 1102 7 0.5546744717053613 1102 8 0.4914357786376584 1102 9 0.6256387378066322 1102 10-11 0.6927566718463822 1102 12-13 0.7047454811728997 1102 14-15 0.9393540216185272 1102 16-17 0.9071288395361776 1102 18-19 0.924912740257767 1102 20-21 0.9464009031484721 1102 22-23 0.9596562875130701 1102 24-25 0.8993922908428829 1102 26-27 0.9282219781226289 1102 28-29 0.90534466026876 1102 30-31 0.9164613623652471 1102 32-33 0.9595736210480439 1102 34-35 1.0762336365224598 1102 36-37 0.8953494106737594 1102 38-39 0.8982193001112151 1102 40-41 0.9791399868637853 1102 42-43 1.0263749279156897 1102 44-45 1.0409422098937995 1102 46-47 1.0083772306645074 1102 48-49 0.8709081613596297 1102 50-51 0.9332249440692593 1102 52-53 0.9184224941689934 1102 54-55 0.8838325299316807 1102 56-57 0.9215472872699593 1102 58-59 0.8532807172274417 1102 60-61 0.7988098835506108 1102 62-63 0.8318635182234431 1102 64-65 0.8431851182631291 1102 66-67 0.8933574641047102 1102 68-69 0.8752280489642459 1102 70-71 0.8233864188132358 1102 72-73 0.8128226301306043 1102 74-75 0.8947948875577367 1102 76-77 0.8552601193398246 1102 78-79 0.77780792556063 1102 80-81 0.8240018696650679 1102 82-83 0.8482296372491973 1102 84-85 0.7040413830957881 1102 86-87 0.7979132626856504 1102 88-89 0.7781872078976733 1102 90-91 0.7898422862969205 1102 92-93 0.8035383567135206 1102 94-95 0.8527134437091952 1102 96-97 0.7893484736868217 1102 98-99 0.8422650805809759 1102 100 0.8244259706556747 1103 1 0.5153168336421352 1103 2 3.9527688058326476 1103 3 2.1977561600618714 1103 4 1.0781018380075338 1103 5 0.47119279390382474 1103 6 0.48824671061652225 1103 7 0.43220714747575073 1103 8 0.4234333234013974 1103 9 0.48630225872713595 1103 10-11 0.4972478751307179 1103 12-13 0.4770534336127099 1103 14-15 0.7481739903877269 1103 16-17 0.7475426263799037 1103 18-19 0.7287573494235033 1103 20-21 0.748534208391888 1103 22-23 0.7590240216772841 1103 24-25 0.6926290681449387 1103 26-27 0.7355475555645725 1103 28-29 0.7842906754276058 1103 30-31 0.7190130868590856 1103 32-33 0.863746080752783 1103 34-35 0.807458698631919 1103 36-37 0.829544252249093 1103 38-39 0.7553737426074463 1103 40-41 0.7064227069547044 1103 42-43 0.7478249896001614 1103 44-45 0.899187787554304 1103 46-47 0.7871306336316763 1103 48-49 0.6803982505592572 1103 50-51 0.6845276794355897 1103 52-53 0.6971133475032403 1103 54-55 0.7184026541263222 1103 56-57 0.6808934257977413 1103 58-59 0.6049042148827013 1103 60-61 0.7099409102327812 1103 62-63 0.7590597373781733 1103 64-65 0.6899774346607614 1103 66-67 0.7377096769744185 1103 68-69 0.8151386459436551 1103 70-71 0.8662310959775965 1103 72-73 0.7386567213653379 1103 74-75 0.7689195266185749 1103 76-77 0.7065606083228388 1103 78-79 0.6869517226284287 1103 80-81 0.7073286510470425 1103 82-83 0.662030186945266 1103 84-85 0.7434672318036881 1103 86-87 0.7119245322443639 1103 88-89 0.6048756685301129 1103 90-91 0.7070104529809917 1103 92-93 0.7521865196576734 1103 94-95 0.7058612338751367 1103 96-97 0.6638036346614271 1103 98-99 0.7044273338127276 1103 100 0.7439241225500446 1104 1 0.4351075921425718 1104 2 3.593328594779532 1104 3 2.0975659405168123 1104 4 0.9666841362672542 1104 5 0.340184743540938 1104 6 0.3487229388346833 1104 7 0.2999241732572955 1104 8 0.38931948606175837 1104 9 0.43517509469674565 1104 10-11 0.4671575208468113 1104 12-13 0.37437733900113557 1104 14-15 0.5296643831684378 1104 16-17 0.5263824003809674 1104 18-19 0.5692469383001821 1104 20-21 0.5230708313156072 1104 22-23 0.505212271091338 1104 24-25 0.6093157380574112 1104 26-27 0.5515412828486177 1104 28-29 0.5584344044100433 1104 30-31 0.43666670612560665 1104 32-33 0.7151942402654541 1104 34-35 0.8092488004220186 1104 36-37 0.6374117552778813 1104 38-39 0.5850570497101089 1104 40-41 0.5496999179738538 1104 42-43 0.5961701927840721 1104 44-45 0.6293409822236313 1104 46-47 0.5893107987150756 1104 48-49 0.5161068749775595 1104 50-51 0.5168151826908343 1104 52-53 0.4831905222900872 1104 54-55 0.5280438411868573 1104 56-57 0.5523067117271552 1104 58-59 0.5040216188388129 1104 60-61 0.535139904786611 1104 62-63 0.6049541655306712 1104 64-65 0.6229963773571185 1104 66-67 0.5761810138328514 1104 68-69 0.6269209351452858 1104 70-71 0.547585051525104 1104 72-73 0.46567921935557166 1104 74-75 0.6065518594121997 1104 76-77 0.6011082141607673 1104 78-79 0.5674168547064653 1104 80-81 0.550698141190729 1104 82-83 0.5799527177710289 1104 84-85 0.5934457188789573 1104 86-87 0.5208240895310148 1104 88-89 0.5386622829618801 1104 90-91 0.5662089266955981 1104 92-93 0.5982689028368249 1104 94-95 0.5613815635890127 1104 96-97 0.5153735120377618 1104 98-99 0.4673679241726738 1104 100 0.3769747685039171 1105 1 0.5439879010228807 1105 2 3.8011208025717416 1105 3 2.2893595523104224 1105 4 1.1097120992952156 1105 5 0.5330219963781886 1105 6 0.49488884500058816 1105 7 0.4522185455516663 1105 8 0.45291372965600374 1105 9 0.5152382747599304 1105 10-11 0.46612908981838075 1105 12-13 0.49538003000382247 1105 14-15 0.6818733453774044 1105 16-17 0.6232526472512134 1105 18-19 0.5908287798820311 1105 20-21 0.6278771961219718 1105 22-23 0.5136900995691676 1105 24-25 0.5460666448083273 1105 26-27 0.5984407797481168 1105 28-29 0.5552356212112599 1105 30-31 0.5336492731081748 1105 32-33 0.6339786090498265 1105 34-35 0.7109359421091668 1105 36-37 0.5562569641230937 1105 38-39 0.587027331680396 1105 40-41 0.5358892241631565 1105 42-43 0.6408338174476995 1105 44-45 0.689404162286813 1105 46-47 0.6799659993702676 1105 48-49 0.576079964950651 1105 50-51 0.5658780117536608 1105 52-53 0.43968168878125624 1105 54-55 0.4874775606205759 1105 56-57 0.5078993173197617 1105 58-59 0.5060679508851464 1105 60-61 0.5269978666445709 1105 62-63 0.5280043585808585 1105 64-65 0.5398186541794061 1105 66-67 0.39382580147764656 1105 68-69 0.45375608198043693 1105 70-71 0.5636819276219782 1105 72-73 0.5069849406612974 1105 74-75 0.5486332914936298 1105 76-77 0.48290884596139705 1105 78-79 0.5676274549170657 1105 80-81 0.5254015458941339 1105 82-83 0.5406196184379226 1105 84-85 0.47131748675072416 1105 86-87 0.5298518185587504 1105 88-89 0.4085324128320167 1105 90-91 0.41232920281587226 1105 92-93 0.4345260690939945 1105 94-95 0.4085887707962179 1105 96-97 0.45037760704185814 1105 98-99 0.45363562044036954 1105 100 0.43754806907721644 1106 1 0.5556728511029974 1106 2 3.9185753928477283 1106 3 2.31208133441098 1106 4 1.058917004255079 1106 5 0.40554921606675975 1106 6 0.3451988226272178 1106 7 0.2748902770413437 1106 8 0.2863106347353934 1106 9 0.24534595827572048 1106 10-11 0.3217834268532158 1106 12-13 0.2953889195899322 1106 14-15 0.4151145706462387 1106 16-17 0.3925966480878813 1106 18-19 0.39475282247734356 1106 20-21 0.38102922672008077 1106 22-23 0.38301796468650906 1106 24-25 0.40689188226858874 1106 26-27 0.3774479100926058 1106 28-29 0.3549654973173162 1106 30-31 0.3712626059932873 1106 32-33 0.47244138297420335 1106 34-35 0.5620957342870767 1106 36-37 0.4787071282816271 1106 38-39 0.41416566932779375 1106 40-41 0.3510739071821831 1106 42-43 0.4614469000797641 1106 44-45 0.391724344261867 1106 46-47 0.4106744396214168 1106 48-49 0.33528916529876795 1106 50-51 0.292542736157813 1106 52-53 0.283825729957222 1106 54-55 0.26186449666411704 1106 56-57 0.30081379427219446 1106 58-59 0.44948244838074913 1106 60-61 0.34605064015809006 1106 62-63 0.3865373527652807 1106 64-65 0.28402395132689406 1106 66-67 0.2860454713677143 1106 68-69 0.26285625416948477 1106 70-71 0.24546626925101833 1106 72-73 0.2814338049979952 1106 74-75 0.21551495167986445 1106 76-77 0.21870325000428892 1106 78-79 0.23265552434548198 1106 80-81 0.21178187824512662 1106 82-83 0.2894729738831714 1106 84-85 0.2719836827922357 1106 86-87 0.3848831375417525 1106 88-89 0.4348457371263592 1106 90-91 0.33903351949430416 1106 92-93 0.37742661404803357 1106 94-95 0.3741316891950497 1106 96-97 0.3440792232283769 1106 98-99 0.36072876313316726 1106 100 0.48491073804471796 1107 1 0.4769705131548676 1107 2 3.561631211941073 1107 3 2.0845795950615873 1107 4 0.9513077235879379 1107 5 0.17551557472238954 1107 6 0.23122975858216677 1107 7 0.016753851186557256 1107 8 0.05774443145765673 1107 9 0.15079987774891634 1107 10-11 0.13708043791703517 1107 12-13 0.16908303515911172 1107 14-15 0.03815529648301208 1107 16-17 0.014293763811004112 1107 18-19 0.03558291578761441 1107 20-21 0.06868497755216652 1107 22-23 0.056245379697060116 1107 24-25 0.10562179919750037 1107 26-27 -0.013427158675845874 1107 28-29 0.02617192557080017 1107 30-31 -0.012977426609815268 1107 32-33 0.20832184879763105 1107 34-35 0.2864393909424905 1107 36-37 0.1328379488368583 1107 38-39 -0.020939473298859923 1107 40-41 0.0036490542362628275 1107 42-43 0.18015846582836303 1107 44-45 0.15234078997448108 1107 46-47 0.10377390653917473 1107 48-49 -0.0017679271296060506 1107 50-51 -0.012340360468861888 1107 52-53 -0.05919164742611116 1107 54-55 0.08244774258245968 1107 56-57 0.05542226463523292 1107 58-59 0.1158646583270837 1107 60-61 0.09271534073342735 1107 62-63 0.314962245092687 1107 64-65 0.18467971289938134 1107 66-67 0.14108303594441907 1107 68-69 0.07709736379682397 1107 70-71 0.043388552255990476 1107 72-73 0.04261668789055051 1107 74-75 0.14484882561373524 1107 76-77 0.12349371061264947 1107 78-79 0.05672703099796905 1107 80-81 0.0519071322856135 1107 82-83 0.06951352190651505 1107 84-85 0.1467129350092371 1107 86-87 0.21001397659435028 1107 88-89 0.1336825807830131 1107 90-91 -0.00486682384073589 1107 92-93 0.11041194324750592 1107 94-95 0.015814660397627023 1107 96-97 0.0198883936273333 1107 98-99 0.16101756076836793 1107 100 0.19777628484485277 1108 1 0.5605054542660959 1108 2 3.688761922513745 1108 3 2.0969918315356217 1108 4 1.0672928076723807 1108 5 0.37014605226330843 1108 6 0.2842879120102779 1108 7 0.1785345009826642 1108 8 0.210163913100871 1108 9 0.15406891314808746 1108 10-11 0.23196643831059305 1108 12-13 0.21012392917250367 1108 14-15 0.16574777478280822 1108 16-17 0.12893420757878715 1108 18-19 0.17266355277874368 1108 20-21 0.18764674810391568 1108 22-23 0.18557788729766145 1108 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2210 78-79 0.31888811451783994 2210 80-81 0.30475378031552935 2210 82-83 0.28678660035755144 2210 84-85 0.409058937055228 2210 86-87 0.3681179096686158 2210 88-89 0.5248034810640263 2210 90-91 0.49921334118902294 2210 92-93 0.500801117614774 2210 94-95 0.31525149239791617 2210 96-97 0.34946483524625194 2210 98-99 0.4641218849217488 2210 100 0.2773582128255221 2211 1 0.6225250180454438 2211 2 4.020788108986718 2211 3 2.2969292304424087 2211 4 1.1481910395882267 2211 5 0.4724942713011586 2211 6 0.4293113895841927 2211 7 0.4648819902354546 2211 8 0.45850280573956326 2211 9 0.4826030377166646 2211 10-11 0.4015663510595431 2211 12-13 0.46473312581071724 2211 14-15 0.43955679595715935 2211 16-17 0.4468015011476254 2211 18-19 0.534961969853029 2211 20-21 0.5194310995040752 2211 22-23 0.4332700430315626 2211 24-25 0.33925238343628195 2211 26-27 0.3674663195281056 2211 28-29 0.3362777626433413 2211 30-31 0.40045805441822324 2211 32-33 0.5953800125817779 2211 34-35 0.5519482857041567 2211 36-37 0.49278287392958475 2211 38-39 0.48730612983430177 2211 40-41 0.45802326794738235 2211 42-43 0.4841159342195027 2211 44-45 0.5609775394606373 2211 46-47 0.5806964378119233 2211 48-49 0.4972961472801458 2211 50-51 0.4208459937575313 2211 52-53 0.4517795861744318 2211 54-55 0.5141284476444525 2211 56-57 0.4912776042866298 2211 58-59 0.4709371082889149 2211 60-61 0.40559917867906137 2211 62-63 0.5817159140499797 2211 64-65 0.5132088431559154 2211 66-67 0.5031866525508377 2211 68-69 0.383106560079149 2211 70-71 0.5261230016651979 2211 72-73 0.49093388801233573 2211 74-75 0.5001006881828474 2211 76-77 0.5061599661325502 2211 78-79 0.4287603598759091 2211 80-81 0.41622491297632536 2211 82-83 0.1462820148152133 2211 84-85 0.3797334919567774 2211 86-87 0.5435715207271663 2211 88-89 0.5751040836026107 2211 90-91 0.5084243161418449 2211 92-93 0.48037292827230615 2211 94-95 0.6045165709794595 2211 96-97 0.35365627431035307 2211 98-99 0.5230020005046931 2211 100 0.5011328858804589 2212 1 0.6942183157694224 2212 2 4.173680500937891 2212 3 2.4577885426748978 2212 4 1.3496674127989152 2212 5 0.8447885094350269 2212 6 0.7012371203811227 2212 7 0.7123665695706549 2212 8 0.5934918612018762 2212 9 0.6056446425856521 2212 10-11 0.5371345359851176 2212 12-13 0.6266424235242809 2212 14-15 0.7199699799256507 2212 16-17 0.8140639864496464 2212 18-19 0.7733270343157699 2212 20-21 0.7122034075449619 2212 22-23 0.6631901693918181 2212 24-25 0.5512851897042808 2212 26-27 0.5070010366954705 2212 28-29 0.4548474072746629 2212 30-31 0.5400617853271328 2212 32-33 0.7525304872791239 2212 34-35 0.7849465551520396 2212 36-37 0.804279586661842 2212 38-39 0.6416049046450567 2212 40-41 0.6217110367591658 2212 42-43 0.6927371828994495 2212 44-45 0.6705478573014716 2212 46-47 0.7762388662882955 2212 48-49 0.5790655814846488 2212 50-51 0.45857176960870305 2212 52-53 0.6414775273512845 2212 54-55 0.4246176248574187 2212 56-57 0.5320579543816208 2212 58-59 0.576662839867133 2212 60-61 0.49028092766957343 2212 62-63 0.7173173037002556 2212 64-65 0.7377044243232334 2212 66-67 0.5384382857675405 2212 68-69 0.622443416151647 2212 70-71 0.7028608132524781 2212 72-73 0.7326908476575298 2212 74-75 0.6803593074131982 2212 76-77 0.6653973252240704 2212 78-79 0.5905013552103213 2212 80-81 0.48359593215302965 2212 82-83 0.6183447964856796 2212 84-85 0.6993708312556812 2212 86-87 0.6424575998742093 2212 88-89 0.6848918598366254 2212 90-91 0.6217943613132952 2212 92-93 0.7727930709093833 2212 94-95 0.8207323261655866 2212 96-97 0.5405130629837629 2212 98-99 0.6360626557706333 2212 100 0.5007431535626239 2213 1 -0.9099070494972921 2213 2 -25.666411664960727 2213 3 -14.654801597374705 2213 4 -6.637473970999203 2213 5 -2.6496406104052 2213 6 -1.813052686528863 2213 7 -1.631755603449129 2213 8 -1.5239742314060294 2213 9 -1.5545328954552886 2213 10-11 -1.5349361681918126 2213 12-13 -1.637745810999462 2213 14-15 -2.028000283066376 2213 16-17 -2.0202911387437297 2213 18-19 -1.911922329281154 2213 20-21 -1.8986309966917219 2213 22-23 -1.9395023681170827 2213 24-25 -2.026179593263855 2213 26-27 -2.046365760662333 2213 28-29 -1.978494588780059 2213 30-31 -2.0515464932332392 2213 32-33 -1.8610418825958916 2213 34-35 -1.7886048604867 2213 36-37 -2.0710459815990347 2213 38-39 -2.123573615625709 2213 40-41 -1.9145264909683704 2213 42-43 -1.991720871794378 2213 44-45 -2.022381725005296 2213 46-47 -1.8164463863493978 2213 48-49 -2.014013381608059 2213 50-51 -1.9347498861033685 2213 52-53 -1.9677617765751805 2213 54-55 -1.9988277918126585 2213 56-57 -2.0472180194583203 2213 58-59 -1.9703984512739723 2213 60-61 -1.9411821445816102 2213 62-63 -1.9052666155604498 2213 64-65 -1.876918522202537 2213 66-67 -2.0435177430772704 2213 68-69 -1.9374885644329467 2213 70-71 -1.9052464708002006 2213 72-73 -2.0919273104978444 2213 74-75 -2.1848390620852918 2213 76-77 -1.9585400248835683 2213 78-79 -1.897327794096796 2213 80-81 -1.886216871212742 2213 82-83 -2.0668648686557987 2213 84-85 -2.0281477697216452 2213 86-87 -1.9254007707702172 2213 88-89 -1.9346774323849338 2213 90-91 -1.8057344712247136 2213 92-93 -1.89654191857257 2213 94-95 -2.0316561293598703 2213 96-97 -1.988788694389097 2213 98-99 -1.9658154495080353 2213 100 -1.9038610487439804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 33.0 5 47.0 6 37.0 7 43.0 8 40.0 9 80.0 10 457.0 11 2356.0 12 6658.0 13 12147.0 14 16192.0 15 17718.0 16 18546.0 17 19281.0 18 19374.0 19 20665.0 20 21981.0 21 23965.0 22 26148.0 23 27983.0 24 28996.0 25 30827.0 26 33684.0 27 38136.0 28 44051.0 29 52105.0 30 62337.0 31 75488.0 32 94021.0 33 124403.0 34 168964.0 35 235419.0 36 353781.0 37 588277.0 38 564959.0 39 3254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.894900973161036 20.495352193547387 9.998344687740861 31.61140214555072 2 12.650341878511966 22.446304734136664 36.22942938500795 28.67392400234342 3 15.230336315870566 26.703180759030047 28.614607857166124 29.451875067933265 4 10.530923411100112 18.506943745505186 36.7862051044627 34.175927738932 5 11.532892682190138 38.62842932988867 34.98950178953434 14.849176198386854 6 30.166548759928702 39.36521502234369 17.45169851613272 13.01653770159489 7 26.179546365634938 33.2355358512463 22.805460013655527 17.779457769463242 8 24.42596499937942 37.13519052854606 20.549382112624542 17.889462359449983 9 24.697220432312108 16.938265186573723 21.78779069767442 36.57672368343975 10-11 23.1659908652394 27.70012722762762 28.848159775932924 20.28572213120006 12-13 23.83582337092523 25.128992941588248 29.242305364634568 21.79287832285195 14-15 21.42128416914612 26.724615002433836 26.287549425023073 25.566551403396975 16-17 20.83457042683295 30.20052446662813 27.261208616247924 21.703696490290998 18-19 20.815583403893765 29.223311775337052 29.258582839044244 20.70252198172494 20-21 21.934840918387795 28.480765341467006 28.52578973425542 21.058604005889777 22-23 21.232823809938672 28.391146753426455 28.683258206757806 21.692771229877074 24-25 21.372980088040453 28.30892930482012 28.543507401005737 21.774583206133695 26-27 21.226686499948023 28.25527028946147 28.66757097450028 21.850472236090226 28-29 20.98503759132911 28.212485127362484 29.012244566282686 21.790232715025716 30-31 21.585902035524214 28.55837869674763 28.42237081630718 21.433348451420972 32-33 20.77798858007816 28.548304102812967 28.97115302270736 21.702554294401516 34-35 20.911954231557083 28.16602332852553 29.071558208041903 21.850464231875485 36-37 21.526589210454997 28.460116321562527 28.45684074032528 21.556453727657196 38-39 21.31637853166069 28.483953351847756 28.65126758418964 21.548400532301912 40-41 21.350887874231255 28.336598915669285 28.55570128220542 21.756811927894038 42-43 21.16567027422401 28.357519845652345 28.693357381682322 21.783452498441317 44-45 21.49006095935171 28.093438675836463 28.557967565328035 21.858532799483793 46-47 21.43405270776107 28.508435038971985 28.490920567571358 21.56659168569558 48-49 21.38235622495914 28.40994075302412 28.50811831824247 21.69958470377427 50-51 21.481028801432565 28.336312968587908 28.313855889611467 21.868802340368056 52-53 21.171322793101133 28.43140969428275 28.233860418157253 22.163407094458865 54-55 21.48644100152261 28.48131859322382 28.086673337431943 21.94556706782162 56-57 21.565324589664698 28.259842300644866 28.121776668958 22.053056440732437 58-59 21.161920497440946 27.961269560767814 28.670587025262595 22.206222916528645 60-61 21.621460872175057 28.324643547994516 28.07255131975543 21.981344260074998 62-63 21.139455748075076 28.52726137024369 28.27186132006046 22.061421561620776 64-65 21.08947734268248 28.061804364146692 28.49118740764357 22.35753088552726 66-67 21.350571530802085 28.198344203293107 28.23039129109865 22.22069297480616 68-69 21.56714091723016 28.123666702236527 28.224976740789252 22.084215639744066 70-71 21.480436829808244 28.12077705408025 28.040571396671155 22.358214719440355 72-73 21.29741853132915 28.27960291630076 28.322504765143492 22.100473787226598 74-75 21.622635707722775 27.88678851595474 28.115045446392294 22.375530329930193 76-77 21.343261456018915 28.223790890822375 28.24194628784357 22.191001365315138 78-79 21.484092977528654 28.19734930846632 28.343501717281654 21.975055996723373 80-81 21.440162424003294 28.023301417202134 28.18881460966869 22.347721549125886 82-83 21.05583503675549 28.240549438282436 28.281504769887032 22.42211075507504 84-85 21.18379765849381 27.95060927693817 28.14230650840924 22.72328655615878 86-87 21.29130358479092 28.1285004685744 28.12092422160925 22.459271725025424 88-89 21.07244362761828 27.812625868993585 28.44754086870719 22.667389634680944 90-91 21.27093484817403 27.88621785692522 28.242955959661913 22.59989133523884 92-93 20.885284330203845 28.210994778290054 28.293524695385003 22.610196196121095 94-95 20.8119778313283 27.75837999688554 28.526272276772062 22.903369895014098 96-97 21.072558841693542 27.909403234291723 28.334737757039274 22.683300166975457 98-99 20.996526001919527 27.800749695556267 28.35139257276228 22.851331729761924 100 21.061146433811608 27.673226247849957 28.23045177031758 23.035175548020863 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26.0 1 18.0 2 14.0 3 23.0 4 24.0 5 31.0 6 64.0 7 127.5 8 199.5 9 241.5 10 216.5 11 196.0 12 215.0 13 255.5 14 297.5 15 349.0 16 457.0 17 565.5 18 709.0 19 904.5 20 1208.5 21 1675.0 22 2376.0 23 3498.5 24 5173.5 25 7836.5 26 11857.0 27 17383.5 28 24483.5 29 32861.5 30 41857.0 31 51342.0 32 61002.5 33 70306.0 34 79004.5 35 87185.0 36 94822.5 37 101560.5 38 107201.5 39 111064.5 40 112667.0 41 112467.0 42 111806.5 43 109782.5 44 105929.0 45 101573.0 46 97527.0 47 94078.0 48 90598.0 49 88644.0 50 94083.5 51 90157.0 52 74871.5 53 66784.5 54 62159.0 55 57353.0 56 52775.0 57 48151.0 58 42885.5 59 37878.0 60 32940.5 61 27684.0 62 22876.0 63 18472.0 64 14484.5 65 11394.5 66 8882.5 67 6683.0 68 4985.0 69 3800.5 70 2806.0 71 2069.5 72 1590.5 73 1105.5 74 765.0 75 523.0 76 288.0 77 137.5 78 74.0 79 42.5 80 24.5 81 16.5 82 12.5 83 7.5 84 2.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.9523649193895173 2 5.986032371550774 3 0.0 4 0.005343168667366158 5 0.16018526888398407 6 0.05449300099800143 7 0.03535274611421718 8 0.04164743796892252 9 0.040293347279247535 10-11 0.04157424387758874 12-13 0.04062272069024956 14-15 0.04309302127276474 16-17 0.033815670196207744 18-19 0.02781375470683754 20-21 0.022269302288303473 22-23 0.02294634763314097 24-25 0.01957941943178695 26-27 0.012918757120412697 28-29 0.01882917999561567 30-31 0.023202526952809208 32-33 0.0015736729636763342 34-35 0.003586510475355367 36-37 0.004446541048527317 38-39 0.006477677083039794 40-41 0.006459378560206348 42-43 0.006861946062542155 44-45 0.00843561902621849 46-47 0.015901416342264354 48-49 0.02473960287081865 50-51 0.02133607762379774 52-53 0.026990321179332477 54-55 0.023440407749644003 56-57 0.01562693849976267 58-59 0.014565624175422816 60-61 0.011930636887406627 62-63 0.01729210407760623 64-65 0.018957269655449794 66-67 0.019890494319955525 68-69 0.027356291636001395 70-71 0.026843932996664908 72-73 0.023568497409478122 74-75 0.024556617642484194 76-77 0.017969149422443723 78-79 0.013961772921919106 80-81 0.012900458597579252 82-83 0.007904961864048562 84-85 0.010539949152064751 86-87 0.00869179834588673 88-89 0.007520692884546203 90-91 0.007484095838879311 92-93 0.004336749911526642 94-95 0.0017017626235104543 96-97 8.600305731719501E-4 98-99 6.770453448374927E-4 100 0.10488713288131102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2732461.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.89972131700488 #Duplication Level Percentage of deduplicated Percentage of total 1 89.49700736907823 75.08773976971594 2 7.819118806502 13.12043777620139 3 1.5277382385587797 3.8453043738124055 4 0.48456580981284614 1.6261974561218628 5 0.22597943609291532 0.9479805855784751 6 0.12138823806177737 0.6110663606727219 7 0.07263195720620907 0.4265660677816695 8 0.05647163182133467 0.37903633377011864 9 0.03452747768790948 0.2607161180215238 >10 0.15395271461637897 2.104285612009424 >50 0.004074063547335413 0.22625833470204304 >100 0.0016692107593539742 0.3249121292979025 >500 3.9377081493002834E-4 0.19583754533184944 >1k 4.37523127191766E-4 0.6138323204726367 >5k 4.3752312719176594E-5 0.22982921650993773 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6261 0.22913410292040765 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4294 0.15714771409363207 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.6597045666891494E-5 0.0 0.0 0.0 6 0.0 3.6597045666891494E-5 0.0 0.0 0.0 7 0.0 3.6597045666891494E-5 0.0 7.319409133378299E-5 0.0 8 0.0 3.6597045666891494E-5 0.0 1.0979113700067448E-4 0.0 9 0.0 3.6597045666891494E-5 0.0 1.0979113700067448E-4 0.0 10-11 0.0 3.6597045666891494E-5 0.0 1.0979113700067448E-4 0.0 12-13 0.0 3.6597045666891494E-5 0.0 1.0979113700067448E-4 0.0 14-15 0.0 3.6597045666891494E-5 0.0 1.2808965983412024E-4 0.0 16-17 0.0 3.6597045666891494E-5 0.0 1.4638818266756598E-4 0.0 18-19 0.0 3.6597045666891494E-5 0.0 1.4638818266756598E-4 0.0 20-21 0.0 3.6597045666891494E-5 0.0 1.4638818266756598E-4 0.0 22-23 0.0 3.6597045666891494E-5 0.0 2.561793196682405E-4 0.0 24-25 0.0 5.489556850033724E-5 0.0 3.110748881685777E-4 0.0 26-27 0.0 7.319409133378299E-5 0.0 5.672542078368182E-4 0.0 28-29 0.0 7.319409133378299E-5 0.0 0.0010613143243398534 0.0 30-31 5.489556850033724E-5 7.319409133378299E-5 0.0 0.0035682119525219207 0.0 32-33 7.319409133378299E-5 7.319409133378299E-5 0.0 0.011034009268567787 0.0 34-35 7.319409133378299E-5 1.2808965983412024E-4 0.0 0.021354376146631188 0.0 36-37 7.319409133378299E-5 1.646867055010117E-4 0.0 0.03784134521956581 0.0 38-39 7.319409133378299E-5 1.8298522833445747E-4 0.0 0.06378865059739187 0.0 40-41 7.319409133378299E-5 1.8298522833445747E-4 0.0 0.09522551282525167 0.0 42-43 7.319409133378299E-5 1.8298522833445747E-4 0.0 0.1284007347222888 0.0 44-45 7.319409133378299E-5 1.8298522833445747E-4 0.0 0.1692430376865397 0.0 46-47 7.319409133378299E-5 1.8298522833445747E-4 0.0 0.21731325717000172 0.0 48-49 7.319409133378299E-5 1.8298522833445747E-4 0.0 0.266627776206138 0.0 50-51 1.0979113700067448E-4 1.8298522833445747E-4 0.0 0.3194007160577955 0.0 52-53 1.0979113700067448E-4 1.8298522833445747E-4 0.0 0.3768031821863148 0.0 54-55 1.0979113700067448E-4 1.8298522833445747E-4 0.0 0.44125057960571074 0.0 56-57 1.0979113700067448E-4 1.8298522833445747E-4 0.0 0.5195316602871917 0.0 58-59 1.0979113700067448E-4 1.8298522833445747E-4 0.0 0.6041989254375452 0.0 60-61 1.0979113700067448E-4 1.8298522833445747E-4 0.0 0.6954719573307725 0.0 62-63 1.0979113700067448E-4 1.8298522833445747E-4 0.0 0.7892335883293485 0.0 64-65 1.0979113700067448E-4 1.8298522833445747E-4 0.0 0.8957309912200028 0.0 66-67 1.0979113700067448E-4 1.8298522833445747E-4 0.0 1.018770258752092 0.0 68-69 1.0979113700067448E-4 1.8298522833445747E-4 0.0 1.1590101377476203 0.0 70-71 1.0979113700067448E-4 1.8298522833445747E-4 0.0 1.3168165986632565 0.0 72-73 1.0979113700067448E-4 1.8298522833445747E-4 0.0 1.4817411849611029 0.0 74-75 1.0979113700067448E-4 1.8298522833445747E-4 0.0 1.6520089399263154 0.0 76-77 1.0979113700067448E-4 2.0128375116790323E-4 0.0 1.842423368531152 0.0 78-79 1.0979113700067448E-4 2.1958227400134897E-4 0.0 2.0584191320571454 0.0 80-81 1.0979113700067448E-4 2.1958227400134897E-4 0.0 2.2991727969767912 0.0 82-83 1.0979113700067448E-4 2.1958227400134897E-4 0.0 2.560585494175397 0.0 84-85 1.0979113700067448E-4 2.1958227400134897E-4 0.0 2.830250825171887 0.0 86-87 1.0979113700067448E-4 2.1958227400134897E-4 0.0 3.1300355247522287 0.0 88 1.0979113700067448E-4 2.1958227400134897E-4 0.0 3.3803593171137667 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCGC 75 8.366035E-4 18.776455 22-23 GAGTACT 3375 0.0 17.458025 12-13 GTATAGG 785 0.0 17.21911 1 TAGATCG 165 0.0015659616 17.100813 5 ATCGACC 195 2.4889293E-4 16.853441 8 GTATCAA 10000 0.0 16.570843 1 AACGCAG 10610 0.0 15.798571 6 CAACGCA 10640 0.0 15.778876 5 TCAACGC 10670 0.0 15.778586 4 ATCAACG 10730 0.0 15.558302 3 CCTATAC 875 0.0 15.048164 3 GTACATG 12545 0.0 15.04483 1 ATAACCG 220 6.154218E-4 14.963212 5 TATCAAC 10925 0.0 14.87893 2 CATGGGG 4560 0.0 14.850706 4 GTATAGA 1015 0.0 14.796936 1 GTATAAG 950 0.0 14.755402 1 TACATGG 12745 0.0 14.480925 2 TCGGTAC 260 1.3262144E-4 14.469388 3 ACATGGG 12445 0.0 14.2077875 3 >>END_MODULE