##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295152_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 208221 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.082306779815678 27.0 22.0 33.0 6.0 33.0 2 21.27491463397064 27.0 2.0 33.0 2.0 33.0 3 23.665230692389336 27.0 15.0 33.0 6.0 33.0 4 23.322902108817075 33.0 6.0 37.0 2.0 37.0 5 25.55036715797158 33.0 15.0 37.0 2.0 37.0 6 26.18577376921636 33.0 15.0 37.0 2.0 37.0 7 26.165780588893533 33.0 15.0 37.0 6.0 37.0 8 26.88102544892206 33.0 15.0 37.0 6.0 37.0 9 27.411798041503978 33.0 15.0 37.0 6.0 37.0 10-11 27.205190638792438 33.0 15.0 37.0 6.0 37.0 12-13 26.0719980213331 33.0 15.0 37.0 4.0 37.0 14-15 27.676939405727566 33.0 15.0 37.0 6.0 40.0 16-17 28.093885823235887 33.0 15.0 37.0 6.0 40.0 18-19 28.20513540901254 33.0 15.0 37.0 6.0 40.0 20-21 27.78608065468901 33.0 15.0 37.0 6.0 40.0 22-23 28.618621560745552 33.0 22.0 37.0 6.0 40.0 24-25 28.87640535776891 33.0 22.0 37.0 6.0 40.0 26-27 27.465952041340692 33.0 15.0 37.0 4.0 40.0 28-29 28.226252395291542 33.0 22.0 37.0 6.0 40.0 30-31 28.875089448230483 33.0 22.0 37.0 6.0 40.0 32-33 27.5639560851211 33.0 18.5 37.0 6.0 40.0 34-35 27.20850922817583 33.0 15.0 37.0 6.0 40.0 36-37 27.500086446612016 33.0 18.5 37.0 6.0 40.0 38-39 27.495514381354425 33.0 18.5 37.0 6.0 40.0 40-41 26.56052223358835 33.0 15.0 37.0 6.0 40.0 42-43 27.011259671214717 33.0 15.0 37.0 6.0 40.0 44-45 26.881834685262294 33.0 15.0 37.0 6.0 40.0 46-47 26.486562354421505 33.0 15.0 37.0 6.0 40.0 48-49 26.32346401179516 27.0 15.0 37.0 6.0 40.0 50-51 26.37417935750957 33.0 15.0 37.0 6.0 40.0 52-53 26.69894727236926 33.0 18.5 37.0 6.0 40.0 54-55 26.627998616854207 33.0 15.0 37.0 6.0 40.0 56-57 26.432206645823427 27.0 15.0 37.0 6.0 40.0 58-59 26.329181494661924 27.0 15.0 37.0 6.0 40.0 60-61 26.227928499046683 27.0 15.0 37.0 6.0 37.0 62-63 25.994827611047874 27.0 15.0 37.0 6.0 37.0 64-65 25.607839747191683 27.0 15.0 37.0 6.0 37.0 66-67 25.852610927812275 27.0 15.0 35.0 6.0 37.0 68-69 25.3570581257414 27.0 15.0 33.0 6.0 37.0 70-71 25.324743901911912 27.0 15.0 33.0 6.0 37.0 72-73 25.194442443365464 27.0 15.0 33.0 6.0 37.0 74-75 25.249501731333538 27.0 15.0 33.0 6.0 37.0 76-77 24.858289989962586 27.0 15.0 33.0 6.0 37.0 78-79 24.41126495406323 27.0 15.0 33.0 6.0 37.0 80-81 24.36958568059898 27.0 15.0 33.0 6.0 37.0 82-83 24.518840558829318 27.0 15.0 33.0 6.0 37.0 84-85 24.36277800990294 27.0 15.0 33.0 6.0 37.0 86-87 23.999205171428436 27.0 15.0 33.0 6.0 37.0 88-89 23.874606787980078 27.0 15.0 33.0 6.0 37.0 90-91 24.011807166424134 27.0 15.0 33.0 6.0 37.0 92-93 23.763916703886736 27.0 15.0 33.0 6.0 37.0 94-95 23.68273373002723 27.0 15.0 33.0 6.0 33.0 96-97 23.849726972783724 27.0 15.0 33.0 6.0 37.0 98-99 23.858006637178768 27.0 15.0 33.0 6.0 37.0 100 23.368641971751167 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.213218985679333 1101 2 10.901153212180525 1101 3 8.23768683585595 1101 4 12.747886199351044 1101 5 10.504257612822265 1101 6 9.544957135687905 1101 7 9.140807823825575 1101 8 8.48562342900049 1101 9 7.092629308740062 1101 10-11 7.2971915526072095 1101 12-13 8.833884002894845 1101 14-15 7.092100307927311 1101 16-17 7.4979915922858495 1101 18-19 7.923600675570107 1101 20-21 6.810638861671848 1101 22-23 7.0409148689107255 1101 24-25 7.117762555450142 1101 26-27 8.091311572819315 1101 28-29 8.098905076563412 1101 30-31 5.398892649509996 1101 32-33 6.746118614209763 1101 34-35 8.622709442695026 1101 36-37 8.214195036864382 1101 38-39 7.955067638780783 1101 40-41 8.718651819878147 1101 42-43 7.941728266704494 1101 44-45 8.31574749606915 1101 46-47 7.093758806823203 1101 48-49 5.401314492234324 1101 50-51 6.607305941697852 1101 52-53 5.719517088293177 1101 54-55 4.3304939898919805 1101 56-57 5.7274882996100835 1101 58-59 5.8548348761223785 1101 60-61 6.360937516602679 1101 62-63 5.887546577287729 1101 64-65 6.349579647841374 1101 66-67 5.765213227067363 1101 68-69 6.733856612575352 1101 70-71 5.967037081415093 1101 72-73 5.298215481838458 1101 74-75 4.9721364802722015 1101 76-77 4.709804008159505 1101 78-79 4.994191726194156 1101 80-81 4.808920363404045 1101 82-83 4.634356694102916 1101 84-85 4.030250479775326 1101 86-87 4.347815950612532 1101 88-89 3.979385965661155 1101 90-91 3.865407858811036 1101 92-93 3.7752205622816497 1101 94-95 4.111885889207883 1101 96-97 4.590293682452312 1101 98-99 4.2434766615576365 1101 100 4.56940380950773 1102 1 6.6305972628328895 1102 2 9.776509017423972 1102 3 7.572043889538847 1102 4 12.087436761148798 1102 5 10.349176464257972 1102 6 9.027104451543089 1102 7 6.4278240535384334 1102 8 7.188345027002992 1102 9 5.808434552185755 1102 10-11 7.082360091933051 1102 12-13 8.184383378675122 1102 14-15 5.471550994569011 1102 16-17 6.593210069189723 1102 18-19 7.138182448354122 1102 20-21 6.294059585766728 1102 22-23 6.142974794004353 1102 24-25 6.5087238538271635 1102 26-27 7.93194827693916 1102 28-29 7.498930045352424 1102 30-31 3.84295007772473 1102 32-33 4.747067428192285 1102 34-35 4.7765296674141275 1102 36-37 4.7707368595859805 1102 38-39 4.308201221802008 1102 40-41 5.676267300527336 1102 42-43 6.28426259878939 1102 44-45 5.976546497317592 1102 46-47 4.920288145150295 1102 48-49 3.634522981622588 1102 50-51 4.7020000740324335 1102 52-53 5.6151225814267605 1102 54-55 5.341342928718451 1102 56-57 6.201608149797352 1102 58-59 5.4802655877328625 1102 60-61 5.772722785017162 1102 62-63 3.9153742926435307 1102 64-65 5.515584641599176 1102 66-67 3.4351008675168018 1102 68-69 5.513856612575353 1102 70-71 4.430769915372647 1102 72-73 4.519850937518857 1102 74-75 3.6828605751535974 1102 76-77 2.911239713527795 1102 78-79 3.9278496537846657 1102 80-81 3.738233721706166 1102 82-83 3.2886762946022934 1102 84-85 2.7391268842697087 1102 86-87 3.0525725049196453 1102 88-89 3.1967892116037255 1102 90-91 3.022948433093184 1102 92-93 3.090801086626218 1102 94-95 2.7739458143015163 1102 96-97 2.8655121593561788 1102 98-99 3.2493568113703724 1102 100 3.310652248958416 1103 1 6.284495426233672 1103 2 10.14459595470423 1103 3 7.374438598559575 1103 4 11.346704580095029 1103 5 9.93623913482567 1103 6 8.557697228077888 1103 7 7.740783510672159 1103 8 7.944276480301248 1103 9 6.968019534852388 1103 10-11 7.083931158734124 1103 12-13 7.798958158012766 1103 14-15 7.7457156738402695 1103 16-17 7.342803165346879 1103 18-19 6.652081103481599 1103 20-21 7.7870696907503785 1103 22-23 5.949485255489861 1103 24-25 4.319581179325652 1103 26-27 5.462391076830983 1103 28-29 6.314844779942941 1103 30-31 5.250648059186634 1103 32-33 7.02381858989661 1103 34-35 6.321768523657827 1103 36-37 3.622235396699054 1103 38-39 5.6482794063470365 1103 40-41 6.194156317811526 1103 42-43 7.0463258840154595 1103 44-45 5.787349732879267 1103 46-47 6.068971546915591 1103 48-49 5.0569186740967105 1103 50-51 5.790250264576528 1103 52-53 5.99793673332114 1103 54-55 4.20082464877844 1103 56-57 5.090996687648012 1103 58-59 4.647300229878759 1103 60-61 4.633711647407441 1103 62-63 1.7721490572293739 1103 64-65 3.85325336532253 1103 66-67 3.7471825924940596 1103 68-69 5.090005409074259 1103 70-71 3.0090380539412287 1103 72-73 3.676817475517037 1103 74-75 3.043950241517038 1103 76-77 2.7691159459178287 1103 78-79 2.633647111557636 1103 80-81 3.912941758979052 1103 82-83 2.9460829279954517 1103 84-85 3.176136694217341 1103 86-87 2.5785307573229588 1103 88-89 2.524370162111431 1103 90-91 2.203596528881544 1103 92-93 1.4277223857681562 1103 94-95 1.8369114180189712 1103 96-97 2.5269214480735087 1103 98-99 2.09891794529214 1103 100 1.5829754117445418 1104 1 6.091089356049704 1104 2 9.359301360328672 1104 3 6.916946095115211 1104 4 11.382515828980676 1104 5 8.868516872081528 1104 6 7.6088460245767955 1104 7 3.6334004164181692 1104 8 5.886456762333829 1104 9 5.818833012443765 1104 10-11 6.641034145199804 1104 12-13 7.64846733622818 1104 14-15 7.296915122742128 1104 16-17 7.040676777471038 1104 18-19 6.4961932681626955 1104 20-21 7.132027750560738 1104 22-23 6.308970424466278 1104 24-25 5.53123477767236 1104 26-27 7.576265276523014 1104 28-29 6.731405076563412 1104 30-31 6.078938945806293 1104 32-33 7.03667416976532 1104 34-35 7.622061294546878 1104 36-37 6.4949357776051215 1104 38-39 6.739836157299305 1104 40-41 6.635318486544811 1104 42-43 6.69853382226005 1104 44-45 5.919451199772858 1104 46-47 6.203018066082464 1104 48-49 4.6397404181602475 1104 50-51 5.010778163920072 1104 52-53 5.390720791996884 1104 54-55 3.881142138040129 1104 56-57 5.519247558869342 1104 58-59 3.0278441353816348 1104 60-61 3.3872801091952702 1104 62-63 3.33125028099143 1104 64-65 2.4005981663598916 1104 66-67 2.684796560400695 1104 68-69 3.6904769829457216 1104 70-71 1.71009263697065 1104 72-73 2.2916414077643843 1104 74-75 2.7121364802722 1104 76-77 2.917674378529874 1104 78-79 3.11599728174971 1104 80-81 2.721559252292934 1104 82-83 2.908245582991807 1104 84-85 2.6990467760716257 1104 86-87 1.0203159506125274 1104 88-89 0.3075804101056008 1104 90-91 1.634111562514743 1104 92-93 1.777211303022387 1104 94-95 1.9739692225412142 1104 96-97 2.1282566454152736 1104 98-99 1.9912081430391204 1104 100 0.36218158728550875 1105 1 5.664330096790444 1105 2 -2.1966245655972507 1105 3 0.7554646136337269 1105 4 8.025663977128822 1105 5 8.155368723933378 1105 6 8.04940158013235 1105 7 4.967474490492243 1105 8 5.890067873444938 1105 9 4.659295975406728 1105 10-11 4.599413774829429 1105 12-13 4.87055066956151 1105 14-15 5.560989196816198 1105 16-17 6.302436036730299 1105 18-19 6.2980451200145495 1105 20-21 6.461749972782961 1105 22-23 2.1331370911329444 1105 24-25 3.6188736665612495 1105 26-27 6.681311572819311 1105 28-29 5.753349521007856 1105 30-31 4.844448205065554 1105 32-33 6.651674169765322 1105 34-35 6.149376109361693 1105 36-37 5.771972814642162 1105 38-39 3.4417343054474507 1105 40-41 5.0630962643225885 1105 42-43 5.079506044482272 1105 44-45 5.862414162735821 1105 46-47 5.170425473489868 1105 48-49 4.581314492234323 1105 50-51 5.270639275031186 1105 52-53 5.258405977182065 1105 54-55 4.661605101003094 1105 56-57 4.7608216329434185 1105 58-59 1.9837237650112662 1105 60-61 4.465381961047125 1105 62-63 -0.08356453382338458 1105 64-65 -0.8170870188252941 1105 66-67 0.5296576715118064 1105 68-69 2.06941216813091 1105 70-71 2.6003704147484257 1105 72-73 2.0193265929495716 1105 74-75 2.2554698136055364 1105 76-77 0.9742484526039483 1105 78-79 2.599747281749707 1105 80-81 0.5989203634040443 1105 82-83 0.695467805214026 1105 84-85 0.9769171464419948 1105 86-87 -0.23551738272080414 1105 88-89 1.2193859656611536 1105 90-91 1.2798523032554812 1105 92-93 1.2029983400594269 1105 94-95 0.8141081114301052 1105 96-97 1.4636270157856401 1105 98-99 1.4023655504465253 1105 100 1.789403809507732 1106 1 6.199885652345998 1106 2 10.101676087997518 1106 3 6.555290321694731 1106 4 11.053812125276973 1106 5 9.830662841580441 1106 6 8.774151035470041 1106 7 5.990698891363703 1106 8 6.886320596756484 1106 9 6.281387478674702 1106 10-11 6.053139265025507 1106 12-13 7.49630230354844 1106 14-15 7.274583968058028 1106 16-17 7.545987234987379 1106 18-19 6.758720501278162 1106 20-21 7.969854547946355 1106 22-23 5.982853866732071 1106 24-25 4.986912882247523 1106 26-27 7.552640548854168 1106 28-29 5.919994401182148 1106 30-31 5.343576745370566 1106 32-33 7.338144758000613 1106 34-35 6.730312928533806 1106 36-37 5.499837738389438 1106 38-39 6.88260576514244 1106 40-41 6.151658355825859 1106 42-43 5.6651051730225745 1106 44-45 3.5403662324525946 1106 46-47 5.485153142335186 1106 48-49 5.705236060861775 1106 50-51 6.127676312068221 1106 52-53 5.125878744066597 1106 54-55 4.8910822251861 1106 56-57 4.413174574119889 1106 58-59 4.820259712723683 1106 60-61 4.438410283487208 1106 62-63 4.555106490141757 1106 64-65 4.916529538908911 1106 66-67 2.4582851224921995 1106 68-69 2.38117687401326 1106 70-71 2.0383878439423277 1106 72-73 2.0643810591805085 1106 74-75 2.124445848463921 1106 76-77 2.753050195523336 1106 78-79 3.0975468460198634 1106 80-81 2.6839094701578574 1106 82-83 1.3338991777630476 1106 84-85 1.8576796736751113 1106 86-87 2.4610839244687384 1106 88-89 2.5629589504106107 1106 90-91 2.287434002601888 1106 92-93 2.209468928294722 1106 94-95 0.8837595275521082 1106 96-97 0.7777664493368412 1106 98-99 1.1138252454356312 1106 100 1.6745454217081672 1107 1 5.868712146226489 1107 2 9.984551340330274 1107 3 6.165006250302795 1107 4 10.771514709070267 1107 5 9.222678539148163 1107 6 7.96170779563511 1107 7 3.579084761670547 1107 8 5.793509198138963 1107 9 5.472355730626312 1107 10-11 4.328206671397705 1107 12-13 6.035983834908286 1107 14-15 5.57671273893283 1107 16-17 6.700868962096266 1107 18-19 5.932518362155175 1107 20-21 5.575827246601051 1107 22-23 5.777667928665942 1107 24-25 4.380954300110563 1107 26-27 6.448942962308152 1107 28-29 6.149166655637089 1107 30-31 4.689012639910764 1107 32-33 4.235108295035296 1107 34-35 6.5000960517663025 1107 36-37 6.099467415074123 1107 38-39 5.859224106263387 1107 40-41 7.153077065858469 1107 42-43 5.777555000565783 1107 44-45 3.858416082582231 1107 46-47 3.360725449491788 1107 48-49 3.8565580727478803 1107 50-51 4.670672872343399 1107 52-53 3.9669996896850677 1107 54-55 5.238588542327786 1107 56-57 4.9919447430946065 1107 58-59 2.8312423635233834 1107 60-61 2.688938476525884 1107 62-63 0.7573161957182535 1107 64-65 2.1591524820146404 1107 66-67 4.2810663588168225 1107 68-69 5.03048728212179 1107 70-71 4.221596716644278 1107 72-73 2.1534374640798823 1107 74-75 2.2930700055901774 1107 76-77 2.666259491720819 1107 78-79 3.9323294751742353 1107 80-81 1.8484116041047898 1107 82-83 1.3413809321638688 1107 84-85 1.9126046914383963 1107 86-87 1.9484566993526293 1107 88-89 2.0678620875714024 1107 90-91 1.1030440479159083 1107 92-93 2.1015128588979195 1107 94-95 2.347731821533298 1107 96-97 2.2686354151136996 1107 98-99 2.0676595269284057 1107 100 0.8034762837097951 1108 1 3.6418100509714293 1108 2 -2.667369124589234 1108 3 0.4799090580781744 1108 4 5.851597539557233 1108 5 5.8322530309207785 1108 6 6.359619220453084 1108 7 3.9613118329893773 1108 8 4.782576464510232 1108 9 4.618952332795043 1108 10-11 2.861521449514427 1108 12-13 1.916759146079265 1108 14-15 3.591092289599704 1108 16-17 4.091588384954811 1108 18-19 3.4340703204727347 1108 20-21 4.574854211041835 1108 22-23 3.9524268964021303 1108 24-25 2.905712154533756 1108 26-27 4.644747998936147 1108 28-29 5.674002441970057 1108 30-31 4.2767506105638375 1108 32-33 4.235900973889031 1108 34-35 2.4708537725919335 1108 36-37 1.0489831239205074 1108 38-39 2.3354571004073605 1108 40-41 4.990874042100369 1108 42-43 2.892255185375742 1108 44-45 2.829378652999278 1108 46-47 4.755328108083226 1108 48-49 4.575655843895262 1108 50-51 2.102208576291204 1108 52-53 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1109 12-13 3.371950769212731 1109 14-15 1.958433143004541 1109 16-17 2.1439507352853546 1109 18-19 2.242414826043447 1109 20-21 2.4660150450300904 1109 22-23 2.9573573203307504 1109 24-25 3.453148703930456 1109 26-27 5.121954323193002 1109 28-29 4.268431733264958 1109 30-31 3.642803959923551 1109 32-33 5.270418564384151 1109 34-35 1.1275524920223816 1109 36-37 2.5364571195748944 1109 38-39 2.25577516244795 1109 40-41 3.2233852528626983 1109 42-43 -0.3272353605999392 1109 44-45 1.8938740531194753 1109 46-47 2.74520374952375 1109 48-49 1.354727546544236 1109 50-51 3.274371213247427 1109 52-53 2.244036271153167 1109 54-55 3.015162649583065 1109 56-57 3.5341848616429665 1109 58-59 1.629618134717294 1109 60-61 -0.34826527363150106 1109 62-63 0.4451848433564862 1109 64-65 2.130740584562851 1109 66-67 1.5057513436592913 1109 68-69 0.7882960744587564 1109 70-71 -0.459221015246591 1109 72-73 -0.4479678764076773 1109 74-75 -0.1684066188807627 1109 76-77 -0.8047450700667049 1109 78-79 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-15.97869694837529 2213 46-47 -15.584018970954578 2213 48-49 -4.8085620509755564 2213 50-51 -22.020471836079928 2213 52-53 -16.611594022817933 2213 54-55 -16.107283787885795 2213 56-57 -16.10806725594547 2213 58-59 -16.71405401276651 2213 60-61 -17.873136557471398 2213 62-63 -15.989737373329557 2213 64-65 -16.069309241047513 2213 66-67 -7.1597250445375735 2213 68-69 -21.08614338742465 2213 70-71 -15.699629585251573 2213 72-73 -7.673636370013394 2213 74-75 -2.865024013554958 2213 76-77 -1.319208337519509 2213 78-79 -2.9044502491144897 2213 80-81 -1.964783340299661 2213 82-83 -2.2352729355267122 2213 84-85 -1.4066631004715866 2213 86-87 -2.3539124444492003 2213 88-89 -1.9713547750795861 2213 90-91 -1.4631106597074819 2213 92-93 -2.6799646229035368 2213 94-95 -1.9855215181995263 2213 96-97 -1.6603236014983054 2213 98-99 -2.7205974125164367 2213 100 -2.4017073016033805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59.0 3 81.0 4 491.0 5 1535.0 6 2062.0 7 2088.0 8 1901.0 9 1999.0 10 2454.0 11 3209.0 12 4067.0 13 5206.0 14 5166.0 15 5493.0 16 5702.0 17 5397.0 18 5282.0 19 5225.0 20 5237.0 21 5260.0 22 5522.0 23 5848.0 24 6213.0 25 6532.0 26 6861.0 27 6752.0 28 6941.0 29 7506.0 30 7747.0 31 8651.0 32 9897.0 33 11420.0 34 12828.0 35 13883.0 36 13400.0 37 8456.0 38 1846.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.424189197666394 22.63712021412291 11.705141877339356 22.233548710871336 2 13.523868296884844 26.92881294917782 33.88873889510292 25.65857985883442 3 20.99455173794938 33.873709182966095 25.2668929501235 19.86484612896103 4 13.593943494746519 24.390710975500774 28.95160157795963 33.06374395179308 5 11.253377836968594 44.41061234865356 30.812504489967342 13.5235053244105 6 29.010977270906224 35.51667957586603 18.537432226703345 16.9349109265244 7 22.250463441916153 38.6507932340106 20.871953493012924 18.226789831060326 8 25.054340944582787 38.53247794707298 19.909888996750094 16.503292111594142 9 25.168037746209613 24.20709571796097 18.445511027953753 32.179355507875655 10-11 20.84182187649655 35.51908466108386 22.104295828425727 21.534797633993865 12-13 24.34866352623051 33.21172191044392 22.518322191580914 19.92129237174466 14-15 24.811592546312774 32.41283638621385 21.112545689294613 21.663025378178766 16-17 19.953750451247014 35.54701601469104 25.77209017615637 18.727143357905586 18-19 21.801936733376373 33.95816655907037 26.423499031633312 17.816397675919948 20-21 21.67713390020655 33.305199366723635 25.392128081193576 19.62553865187624 22-23 20.044665355186783 33.616435622795194 22.678795723791083 23.660103298226947 24-25 21.15654309675613 34.36236144516983 21.36372313213454 23.1173723259395 26-27 22.589266623904212 30.894537096429335 22.8391597177678 23.677036561898653 28-29 22.348073043165375 30.479201202822004 25.492887158333012 21.679838595679605 30-31 23.801508587070835 33.138344177246395 23.634195311202692 19.42595192448007 32-33 19.079975772131075 32.54892521893688 26.065325317329542 22.305773691602504 34-35 17.674123598251096 32.95624099943332 26.34014213746887 23.029493264846714 36-37 22.778325174110826 32.51844553234078 23.821050535457058 20.882178758091335 38-39 24.069744738572837 30.27761221637619 23.435444755014796 22.217198290036173 40-41 20.77328192537231 32.865404850879116 23.722470852266284 22.638842371482294 42-43 23.681160763953272 31.32455652388899 24.027803531326615 20.96647918083112 44-45 24.97753564044666 29.79507869606301 23.66752232918719 21.55986333430314 46-47 21.043279376206726 31.921200202050766 27.24839167079059 19.787128750951922 48-49 22.584817780273443 31.174639145279432 26.47512997788997 19.765413096557154 50-51 20.186072374636698 31.795516036771694 26.149501481525338 21.868910107066274 52-53 19.203514632124925 32.036254720141876 23.329529509860972 25.430701137872227 54-55 20.546096003687577 33.286732060409435 21.758995438647144 24.408176497255845 56-57 22.110356510668947 30.233413301819322 22.798119303513374 24.858110883998357 58-59 21.851134332153997 29.09919141901848 25.436765875538867 23.61290837328865 60-61 23.249598070739548 31.200763665594856 23.754019292604504 21.795618971061092 62-63 19.439369265599556 31.586616753386025 25.206537712992017 23.767476268022403 64-65 18.129286546992414 31.933040287864184 25.879010239769062 24.05866292537434 66-67 21.91800419820873 31.1032507751393 23.755645505425356 23.223099521226604 68-69 24.081352184672806 29.43527120775691 23.08967909448659 23.393697513083694 70-71 21.202718057037792 31.572889426738403 23.1121112264037 24.112281289820103 72-73 23.601433187523355 30.510921094087735 23.621216450882553 22.266429267506357 74-75 24.754024503083475 29.181825668651264 22.886257829796097 23.177891998469164 76-77 21.538042168748007 30.700484091191193 26.29036222871788 21.471111511342922 78-79 23.3083811081138 30.46382743308675 25.85321433292372 20.374577125875735 80-81 21.368170204402514 30.734571540880502 25.40413718553459 22.49312106918239 82-83 20.265172165645374 31.04021438626842 23.613746114127736 25.080867333958466 84-85 21.454617152204396 31.59784429038791 22.190469699330116 24.757068858077584 86-87 22.832782388436677 29.27243883062851 23.21284985403381 24.681928926901 88-89 22.954195292590043 27.846442310487507 25.40504268452214 23.794319712400313 90-91 23.758026930926178 29.55936913509367 24.09333674271847 22.58926719126168 92-93 21.011357881791216 30.167112783042754 24.813475125997734 24.008054209168293 94-95 19.922958025406945 30.50668985171231 25.294160266628023 24.276191856252716 96-97 22.821789232913307 29.801628419814453 23.756765961146897 23.619816386125343 98-99 23.76344423342833 28.77409826808592 23.322562663339745 24.13989483514601 100 21.73927746697972 30.064639554769702 24.590084736755653 23.605998241494923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2059.0 1 2097.5 2 1829.0 3 1261.0 4 781.5 5 484.0 6 343.0 7 284.0 8 300.5 9 312.0 10 306.5 11 333.5 12 390.0 13 434.0 14 497.5 15 582.0 16 641.0 17 726.5 18 811.0 19 861.5 20 913.0 21 995.5 22 1069.0 23 1157.0 24 1305.5 25 1464.5 26 1670.0 27 1894.5 28 2168.5 29 2455.0 30 2700.0 31 2989.5 32 3261.5 33 3502.5 34 3742.5 35 3891.0 36 3972.5 37 4092.5 38 4274.5 39 4438.5 40 4509.5 41 4559.0 42 4660.5 43 4698.0 44 4810.5 45 5108.0 46 5788.0 47 7038.0 48 8977.5 49 12345.5 50 16678.0 51 16445.0 52 11456.5 53 7419.5 54 5390.0 55 4352.5 56 3579.5 57 2872.5 58 2240.0 59 1807.0 60 1465.5 61 1218.0 62 991.5 63 783.5 64 630.0 65 499.5 66 381.0 67 299.5 68 246.0 69 192.0 70 150.5 71 105.0 72 63.5 73 49.5 74 41.0 75 29.0 76 22.0 77 16.0 78 11.5 79 7.5 80 4.0 81 6.5 82 5.0 83 0.5 84 0.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.130303859841225 2 25.289956344460933 3 6.473890721877236 4 24.763592529091685 5 13.092819648354393 6 10.09312221149644 7 8.54764889228272 8 8.9697965142805 9 5.541708089001589 10-11 7.236782072893705 12-13 10.486454296156488 14-15 7.5652791985438546 16-17 8.204743997963702 18-19 7.0098597163590615 20-21 8.388683177969561 22-23 6.131706215991663 24-25 5.654328814096561 26-27 10.153154580950048 28-29 6.569222124569568 30-31 4.558618006829283 32-33 9.212567416350897 34-35 7.622670143741505 36-37 7.146973648191104 38-39 6.530081019685814 40-41 8.945543437021241 42-43 6.758684282565159 44-45 6.735391723217159 46-47 6.349263522891543 48-49 4.208989487131461 50-51 6.72146421350392 52-53 4.676041321480542 54-55 5.187517109225294 56-57 5.311664049255358 58-59 3.690549944530091 60-61 4.4092574716287025 62-63 3.268162193054495 64-65 4.83716820109403 66-67 2.6488682697710604 68-69 5.296776021630864 70-71 2.5720268368704406 72-73 1.682106992090135 74-75 0.8635056022207174 76-77 1.3372810619486026 78-79 1.9037465001128608 80-81 2.257697350411342 82-83 0.8954428227700376 84-85 1.3509684421840256 86-87 0.5534984463622785 88-89 0.8755120761114393 90-91 0.4562460078474313 92-93 0.42070684513089457 94-95 0.5719884161539903 96-97 0.5813534657887534 98-99 0.49106478213052474 100 0.5892777385566298 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 208221.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.42823887090933 #Duplication Level Percentage of deduplicated Percentage of total 1 92.74432268816129 63.46330666826194 2 4.307191289308264 5.894670288149715 3 1.0616383191243781 2.179381214866608 4 0.4804014137753418 1.3149209078296653 5 0.30122585910039335 1.0306177520308295 6 0.1992070670542809 0.817883326149815 7 0.13323261661530095 0.6381811320603668 8 0.10830371579358967 0.5928826027944663 9 0.08046245142227666 0.4955313461456236 >10 0.4926389056391096 6.437572739604924 >50 0.046681050850774064 2.2319336363872884 >100 0.03376927082821953 5.547211431240271 >500 0.005959283087332859 2.9611860090558517 >1k 0.004966069239444049 6.394720945422655 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2971 1.426849357173388 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2226 1.069056435229876 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2001 0.9609981702133791 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1142 0.5484557273281754 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1069 0.5133968235672675 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 917 0.4403974623116784 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 808 0.3880492361481311 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 772 0.37075991374549155 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 636 0.3054446957799646 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 625 0.3001618472680469 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 599 0.28767511442169613 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 446 0.2141954942104783 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 439 0.21083368152107615 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 394 0.18922202851777678 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 372 0.17865633149394153 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 334 0.16040649118004427 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 332 0.15944597326878654 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 325 0.1560841605793844 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 322 0.15464338371249778 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 309 0.14840001728932242 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 307 0.14743949937806464 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 292 0.14023561504363152 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 280 0.13447250757608503 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 279 0.13399224862045617 No Hit ANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 254 0.12198577472973426 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 252 0.12102525681847653 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 248 0.11910422099596102 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 247 0.11862396204033215 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 245 0.1176634441290744 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.4012947781443754E-4 0.0 20-21 0.0 0.0 0.0 7.203884334433127E-4 0.0 22-23 0.0 0.0 0.0 0.0014407768668866254 0.0 24-25 0.0 0.0 0.0 0.0024012947781443753 0.0 26-27 0.0 0.0 0.0 0.005282848511917626 0.0 28-29 0.0 0.0 0.0 0.015128157102309567 0.0 30-31 0.0 0.0 0.0 0.038900975405938884 0.0 32-33 0.0 0.0 0.0 0.0953314026923317 0.0 34-35 0.0 2.4012947781443754E-4 0.0 0.18778125165089016 0.0 36-37 0.0 4.802589556288751E-4 0.0 0.2922375745001705 0.0 38-39 0.0 4.802589556288751E-4 0.0 0.44928225299081265 0.0 40-41 0.0 4.802589556288751E-4 0.0 0.7307140009893334 0.0 42-43 0.0 4.802589556288751E-4 0.0 0.9081696850942027 0.0 44-45 0.0 4.802589556288751E-4 0.0 1.0731386363527213 0.0 46-47 0.0 4.802589556288751E-4 0.0 1.2966991801979626 2.4012947781443754E-4 48-49 0.0 4.802589556288751E-4 0.0 1.5000888479067913 4.802589556288751E-4 50-51 0.0 4.802589556288751E-4 0.0 1.7027581271821766 4.802589556288751E-4 52-53 0.0 4.802589556288751E-4 0.0 1.9551342083651504 4.802589556288751E-4 54-55 0.0 4.802589556288751E-4 0.0 2.2041484768587223 4.802589556288751E-4 56-57 0.0 4.802589556288751E-4 0.0 2.444518084150974 4.802589556288751E-4 58-59 0.0 4.802589556288751E-4 0.0 2.729551774316712 4.802589556288751E-4 60-61 0.0 4.802589556288751E-4 0.0 3.000898084247026 4.802589556288751E-4 62-63 0.0 4.802589556288751E-4 0.0 3.2897738460577948 4.802589556288751E-4 64-65 0.0 4.802589556288751E-4 0.0 3.651168710168523 4.802589556288751E-4 66-67 0.0 4.802589556288751E-4 0.0 4.0308134145931485 4.802589556288751E-4 68-69 0.0 4.802589556288751E-4 0.0 4.470490488471384 4.802589556288751E-4 70-71 0.0 4.802589556288751E-4 0.0 5.207928114839522 4.802589556288751E-4 72-73 0.0 4.802589556288751E-4 0.0 5.790482228017346 4.802589556288751E-4 74-75 0.0 4.802589556288751E-4 0.0 6.313724360175007 4.802589556288751E-4 76-77 0.0 4.802589556288751E-4 0.0 6.918850644267389 4.802589556288751E-4 78-79 0.0 4.802589556288751E-4 0.0 7.47571090331907 4.802589556288751E-4 80-81 0.0 4.802589556288751E-4 0.0 8.104129746759453 4.802589556288751E-4 82-83 0.0 4.802589556288751E-4 0.0 8.96115185307918 4.802589556288751E-4 84-85 0.0 4.802589556288751E-4 0.0 9.736770066419814 4.802589556288751E-4 86-87 0.0 4.802589556288751E-4 0.0 10.441069824849558 4.802589556288751E-4 88 0.0 4.802589556288751E-4 0.0 11.068528150378684 4.802589556288751E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCCTAC 30 0.003477385 62.51256 1 GCCGCTC 30 0.008319678 50.150406 8 CTCGTGT 15 0.009787105 48.141064 16-17 TAGATGT 60 2.9740757E-5 47.061726 4 GTACATA 125 3.783498E-10 45.00904 1 TTAGATG 70 7.38699E-5 40.33862 3 CTGTGCG 75 4.744743E-6 40.120323 9 ACTTAGA 65 0.0015166744 38.469265 1 ATTACAC 60 0.001686556 37.64938 3 TACATAA 120 6.2579056E-7 36.46566 2 TACATGA 140 5.1679308E-8 35.721462 2 GAACATA 80 1.6212727E-4 35.29629 3 TACTGTG 100 8.712923E-7 34.96438 7 AGTACTC 100 1.5101941E-5 33.88444 3 CTTAGAT 75 0.0030610333 33.340034 2 CATAGAA 70 0.0035921477 32.270897 4 GTACAAG 120 2.6214646E-5 31.25628 1 TACAAGA 80 0.004198854 31.256279 2 GCATGCA 65 0.0044711037 30.861788 9 CATGAAA 150 2.3140456E-7 30.119503 4 >>END_MODULE