FastQCFastQC Report
Wed 25 May 2016
SRR1295151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278041
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG2009.328036E-418.474071
GTACATA14800.017.8915541
GTACATG127800.016.2864781
GTATATA20300.015.7742471
TACATGG129950.015.5376212
GTATAAG13200.015.3950591
ACATGGG132500.014.8018483
GTTACAC8750.014.6873853
GTATAGG8150.014.3561281
GTATTAA20700.014.2794741
TAGACTG13750.014.1637285
CATGGGG44400.013.9709534
CTATACA13300.013.8659084
GTATCAA97550.013.8247781
TACACTG21600.013.6399865
GTATTAC11750.013.6262641
CTAGACT11100.013.5933594
CTATACT13700.013.4610644
CCTAGAC9550.013.4570273
CCTATAC10600.013.1782843
Produced by FastQC (version 0.11.5)