##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295151_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3278041 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.01666361097985 33.0 27.0 33.0 15.0 33.0 2 23.22854290108025 33.0 15.0 33.0 2.0 33.0 3 25.47943024507625 33.0 15.0 33.0 15.0 33.0 4 25.801170272122892 33.0 15.0 37.0 2.0 37.0 5 27.508263624524524 37.0 15.0 37.0 2.0 37.0 6 28.47332446421506 33.0 22.0 37.0 6.0 37.0 7 28.314577212426567 33.0 22.0 37.0 6.0 37.0 8 28.98998151639958 33.0 27.0 37.0 6.0 37.0 9 30.014020263932025 37.0 27.0 37.0 15.0 37.0 10-11 29.52438407573304 37.0 27.0 37.0 10.5 37.0 12-13 28.315940069083943 35.0 22.0 37.0 4.0 37.0 14-15 30.251335019909757 37.0 27.0 38.5 10.5 40.0 16-17 30.699650187413763 37.0 27.0 40.0 10.5 40.0 18-19 30.825439645202728 37.0 27.0 40.0 10.5 40.0 20-21 30.38799072372798 37.0 27.0 40.0 6.0 40.0 22-23 31.110933633838016 37.0 27.0 40.0 15.0 40.0 24-25 31.242682291039067 37.0 27.0 40.0 15.0 40.0 26-27 29.85687457844487 37.0 27.0 40.0 4.0 40.0 28-29 30.525616214074198 37.0 27.0 40.0 10.5 40.0 30-31 31.214076181475463 37.0 27.0 40.0 15.0 40.0 32-33 29.98706239488768 37.0 27.0 40.0 6.0 40.0 34-35 29.64363029626536 33.0 27.0 38.5 6.0 40.0 36-37 29.96868404025453 33.0 27.0 37.0 6.0 40.0 38-39 30.09501345468223 35.0 27.0 38.5 6.0 40.0 40-41 29.198608711727523 33.0 22.0 37.0 6.0 40.0 42-43 29.82769465055501 33.0 27.0 37.0 6.0 40.0 44-45 29.77475174959679 33.0 27.0 37.0 6.0 40.0 46-47 29.41861465430115 33.0 22.0 37.0 6.0 40.0 48-49 29.35529070563791 33.0 22.0 37.0 10.5 40.0 50-51 29.310769297882487 33.0 22.0 37.0 6.0 40.0 52-53 29.497892491277565 33.0 24.5 37.0 6.0 40.0 54-55 29.51150366941719 33.0 27.0 37.0 6.0 40.0 56-57 29.503612675985444 33.0 27.0 37.0 6.0 40.0 58-59 29.358768697523917 33.0 22.0 37.0 10.5 40.0 60-61 29.295892272244306 33.0 24.5 37.0 10.5 40.0 62-63 29.218706690977932 33.0 24.5 37.0 10.5 40.0 64-65 28.822038833559432 33.0 24.5 37.0 10.5 40.0 66-67 29.04280803687324 33.0 22.0 37.0 15.0 40.0 68-69 28.601188179159443 33.0 24.5 37.0 10.5 40.0 70-71 28.734729522907127 33.0 22.0 37.0 15.0 38.5 72-73 28.75696399160352 33.0 24.5 37.0 15.0 37.0 74-75 28.84377666417229 33.0 27.0 37.0 15.0 37.0 76-77 28.616162976607065 33.0 27.0 37.0 15.0 37.0 78-79 28.150110233520568 33.0 22.0 37.0 15.0 37.0 80-81 27.97269604010444 33.0 22.0 37.0 15.0 37.0 82-83 28.04740697264006 33.0 22.0 37.0 15.0 37.0 84-85 28.000764633511295 33.0 24.5 33.0 15.0 37.0 86-87 27.74935838203366 33.0 22.0 33.0 15.0 37.0 88-89 27.66780418548761 33.0 22.0 33.0 15.0 37.0 90-91 27.800597674037633 33.0 24.5 33.0 15.0 37.0 92-93 27.72768125841013 33.0 24.5 33.0 15.0 37.0 94-95 27.591750987861346 33.0 22.0 33.0 15.0 37.0 96-97 27.688920303315303 33.0 22.0 33.0 15.0 37.0 98-99 27.730898271254084 33.0 24.5 33.0 15.0 37.0 100 27.399026430724938 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.412333748073294 1101 2 11.484217146086714 1101 3 8.586146661951052 1101 4 13.147575307875005 1101 5 11.018276155498036 1101 6 10.10909536629475 1101 7 9.605689124297424 1101 8 9.135006154071995 1101 9 7.379883351026567 1101 10-11 7.89897806496262 1101 12-13 9.19238843999921 1101 14-15 8.119784502459726 1101 16-17 8.323359888879153 1101 18-19 8.3045269090745 1101 20-21 7.460695919465294 1101 22-23 7.547802196277754 1101 24-25 7.485977245097409 1101 26-27 8.867673013158083 1101 28-29 8.405975942933388 1101 30-31 5.65577330776652 1101 32-33 7.202180971774734 1101 34-35 8.87831658944744 1101 36-37 8.425713670892478 1101 38-39 8.393008564668012 1101 40-41 9.363610466284577 1101 42-43 8.77013165851736 1101 44-45 8.705623537202104 1101 46-47 7.5772528237887435 1101 48-49 5.700064170127021 1101 50-51 7.054208152686748 1101 52-53 6.018164030660696 1101 54-55 4.244915374791244 1101 56-57 6.507451688475552 1101 58-59 6.525031864925747 1101 60-61 7.018756450371534 1101 62-63 6.406612575492648 1101 64-65 7.0209358132135335 1101 66-67 6.056823377908042 1101 68-69 7.09970151700205 1101 70-71 6.590354661708211 1101 72-73 5.729564037786272 1101 74-75 5.165581860988166 1101 76-77 5.185269545183576 1101 78-79 5.332394923463113 1101 80-81 5.142797357394123 1101 82-83 4.719767966262189 1101 84-85 4.420414802726974 1101 86-87 4.258271222155184 1101 88-89 4.186065852993313 1101 90-91 4.0378462554548555 1101 92-93 3.887194476924762 1101 94-95 3.8340012596485202 1101 96-97 3.8685943999336736 1101 98-99 3.614789007933158 1101 100 3.8585538701359283 1102 1 6.718093342949839 1102 2 10.56575276729071 1102 3 8.158894009826682 1102 4 12.672617256812185 1102 5 10.491314418962887 1102 6 9.478954445715747 1102 7 6.521894062551841 1102 8 7.793186486924917 1102 9 6.505579192801907 1102 10-11 7.339474280855185 1102 12-13 8.968631010453695 1102 14-15 6.105476027497961 1102 16-17 7.346390261534253 1102 18-19 7.390887296281175 1102 20-21 6.806936354759493 1102 22-23 6.418816699121923 1102 24-25 6.888758059289728 1102 26-27 8.360240972757133 1102 28-29 7.580258517472522 1102 30-31 4.1843368206621925 1102 32-33 4.389507170887722 1102 34-35 4.995288734888991 1102 36-37 5.073568124906462 1102 38-39 4.4661796374236005 1102 40-41 6.23905409225091 1102 42-43 6.90040776723416 1102 44-45 6.554405915631417 1102 46-47 5.620119125621503 1102 48-49 3.6841319289745904 1102 50-51 5.120410073866843 1102 52-53 5.916105109520057 1102 54-55 5.381880047171787 1102 56-57 6.3018506060421124 1102 58-59 5.97070317556604 1102 60-61 6.045013758073665 1102 62-63 4.221928927339217 1102 64-65 6.179138774123857 1102 66-67 3.8210701188875653 1102 68-69 6.092240350229783 1102 70-71 5.335924262131535 1102 72-73 5.056179739105197 1102 74-75 4.192802833832175 1102 76-77 3.2876880203542136 1102 78-79 4.406256790196615 1102 80-81 4.486091097429075 1102 82-83 3.851995251754012 1102 84-85 2.920706530605436 1102 86-87 3.2748640865423333 1102 88-89 3.3556840306340057 1102 90-91 3.3420123129045898 1102 92-93 3.15451880777249 1102 94-95 3.0021975829953362 1102 96-97 3.1965071531068183 1102 98-99 2.9728602039246255 1102 100 3.2656514214319934 1103 1 6.665788849121981 1103 2 11.025448018601008 1103 3 8.043880995353835 1103 4 12.241625820642096 1103 5 10.57178102852162 1103 6 9.182586580962433 1103 7 8.173075748368543 1103 8 8.17361415925754 1103 9 7.168478623846436 1103 10-11 7.439047977225059 1103 12-13 8.794373531556491 1103 14-15 8.591294676777391 1103 16-17 8.182201359351637 1103 18-19 7.358778361721672 1103 20-21 8.373617534392253 1103 22-23 6.903975657397734 1103 24-25 5.313556706634095 1103 26-27 6.062003059226093 1103 28-29 7.0748123204596 1103 30-31 6.168543291793188 1103 32-33 8.117430294644382 1103 34-35 7.467915982923838 1103 36-37 4.269809325683319 1103 38-39 6.519235501723831 1103 40-41 7.014521987918023 1103 42-43 7.7116432218203705 1103 44-45 5.850539272091101 1103 46-47 6.528947964655007 1103 48-49 5.783979719817971 1103 50-51 6.727900770229631 1103 52-53 6.2362630884656305 1103 54-55 5.026749182694569 1103 56-57 6.134858105587849 1103 58-59 5.6687501786975325 1103 60-61 5.197270583297431 1103 62-63 2.87413578312281 1103 64-65 5.258607410778179 1103 66-67 4.2065542619972085 1103 68-69 5.346289821745433 1103 70-71 3.951053321337355 1103 72-73 4.568226444897878 1103 74-75 3.387155697134194 1103 76-77 3.740628134900451 1103 78-79 3.0687996506432427 1103 80-81 4.160729158213623 1103 82-83 3.504915661704004 1103 84-85 3.326139158585022 1103 86-87 2.8662908762978283 1103 88-89 3.11062079893863 1103 90-91 2.80280736386516 1103 92-93 2.4534785938773283 1103 94-95 2.9330810568566577 1103 96-97 3.117159693819822 1103 98-99 2.938964876627743 1103 100 3.136217828281172 1104 1 6.578339351816652 1104 2 9.983791219245912 1104 3 7.58308448204313 1104 4 12.54179183068403 1104 5 9.883547767668176 1104 6 8.480475693909067 1104 7 4.329845680935932 1104 8 6.839060987006576 1104 9 6.859425335579804 1104 10-11 7.364533628359979 1104 12-13 7.700848222201319 1104 14-15 7.592765025805132 1104 16-17 7.452754251729772 1104 18-19 7.164994169992038 1104 20-21 7.795684909591692 1104 22-23 6.7532858426161475 1104 24-25 6.29610232949468 1104 26-27 8.590585371741192 1104 28-29 7.521508804347782 1104 30-31 6.543301273551219 1104 32-33 7.875514493311041 1104 34-35 8.190495262287168 1104 36-37 6.852029327262088 1104 38-39 7.516867466550746 1104 40-41 7.631361123230555 1104 42-43 7.635295406861768 1104 44-45 7.1052516010876055 1104 46-47 7.083084939684131 1104 48-49 5.883789789020717 1104 50-51 6.057940335158932 1104 52-53 6.280876552980384 1104 54-55 5.011382369872091 1104 56-57 6.120012825033275 1104 58-59 4.00503329997337 1104 60-61 4.564924579140008 1104 62-63 4.139159632115867 1104 64-65 3.0051660513138145 1104 66-67 3.588708959913948 1104 68-69 4.471445605664467 1104 70-71 2.8175475791600384 1104 72-73 2.7467710822233435 1104 74-75 3.3663073834278414 1104 76-77 3.9920774934653984 1104 78-79 4.577696377566291 1104 80-81 3.278616381420587 1104 82-83 3.155077724821311 1104 84-85 3.1350711088202807 1104 86-87 0.9842721678750941 1104 88-89 0.47393640699226935 1104 90-91 2.385316866385214 1104 92-93 2.5870235062914837 1104 94-95 2.7653331720494485 1104 96-97 3.0934448962013725 1104 98-99 2.6351954440041396 1104 100 0.9389360633121591 1105 1 5.90828301345438 1105 2 -2.9952697685138396 1105 3 0.2578982689299316 1105 4 8.845319798233135 1105 5 8.847652877261119 1105 6 8.300256982456364 1105 7 5.583546103177131 1105 8 6.167135401087606 1105 9 5.053881284910862 1105 10-11 5.142873840903857 1105 12-13 5.21031715900747 1105 14-15 6.096318019447789 1105 16-17 6.241459062432874 1105 18-19 6.274273809900944 1105 20-21 7.222693164644362 1105 22-23 2.235828481860853 1105 24-25 4.198124283664903 1105 26-27 7.069201938777908 1105 28-29 6.151392035679024 1105 30-31 5.421653702624187 1105 32-33 7.295610149919828 1105 34-35 6.399166566490603 1105 36-37 6.433619433059597 1105 38-39 4.115075254291522 1105 40-41 5.531111040205602 1105 42-43 5.809151975321772 1105 44-45 6.397512311306787 1105 46-47 5.7350724978016 1105 48-49 5.304380630182116 1105 50-51 5.989011297773985 1105 52-53 5.622589535711196 1105 54-55 5.371498248987752 1105 56-57 5.250589314738175 1105 58-59 1.8879588621709225 1105 60-61 4.52230443016952 1105 62-63 -0.8140738340573961 1105 64-65 -0.3971909085495504 1105 66-67 0.9944329968244752 1105 68-69 2.985683495881755 1105 70-71 3.125451080440996 1105 72-73 2.759567481312441 1105 74-75 2.7997163880772398 1105 76-77 1.8350445681404146 1105 78-79 3.3358275451343715 1105 80-81 1.7509940975226819 1105 82-83 0.9377856430298692 1105 84-85 1.3168295180621428 1105 86-87 -0.2957037548879775 1105 88-89 1.6246993470245314 1105 90-91 1.3503686383749667 1105 92-93 1.7081506293581867 1105 94-95 0.9696561035419968 1105 96-97 2.0475705248188873 1105 98-99 1.951185931716445 1105 100 1.9287983604940564 1106 1 6.291277732114857 1106 2 10.544017365545631 1106 3 6.87746814040074 1106 4 12.037688186026688 1106 5 10.060915033150636 1106 6 9.510951024768357 1106 7 6.720664517779518 1106 8 7.625847447443903 1106 9 6.952201692558273 1106 10-11 5.906134522307724 1106 12-13 8.074916606362589 1106 14-15 8.020102277302772 1106 16-17 7.92514351484337 1106 18-19 7.368212611086658 1106 20-21 8.377746705707828 1106 22-23 6.363694527711612 1106 24-25 5.267660021750295 1106 26-27 8.027858586601802 1106 28-29 6.672721820672201 1106 30-31 5.589745162862865 1106 32-33 7.558985812480909 1106 34-35 7.016277522493478 1106 36-37 6.215668288322554 1106 38-39 7.406221858378725 1106 40-41 7.078983481877252 1106 42-43 6.527841396940413 1106 44-45 4.820409819234545 1106 46-47 6.3283009224381495 1106 48-49 6.059896996972867 1106 50-51 6.796676966769191 1106 52-53 5.937024147059756 1106 54-55 5.62927880339436 1106 56-57 5.233026476755423 1106 58-59 5.295727946228094 1106 60-61 5.168571051644154 1106 62-63 5.237498427229124 1106 64-65 5.5212285960232705 1106 66-67 3.2689517496011007 1106 68-69 3.4724288751884025 1106 70-71 3.0410678367257518 1106 72-73 3.021544766573715 1106 74-75 3.000235062342295 1106 76-77 3.7413662434625046 1106 78-79 4.408641543556477 1106 80-81 3.8977404055637983 1106 82-83 2.171396237113541 1106 84-85 2.8609906601949007 1106 86-87 3.5488743315743285 1106 88-89 3.611084530550123 1106 90-91 3.383351857962264 1106 92-93 3.1250332794493403 1106 94-95 1.7302798205479881 1106 96-97 1.7692674471651948 1106 98-99 2.178578485755388 1106 100 3.068028851325437 1107 1 6.245967886472435 1107 2 10.68612563766019 1107 3 6.370933412577777 1107 4 11.73612784602911 1107 5 9.668990373247539 1107 6 8.28032708627423 1107 7 3.812388377231837 1107 8 6.474751317024182 1107 9 5.945587915340656 1107 10-11 4.765029284843738 1107 12-13 6.361649924706288 1107 14-15 6.432636460497893 1107 16-17 7.192461768730752 1107 18-19 6.51005705952252 1107 20-21 5.785056817687796 1107 22-23 6.00071714331369 1107 24-25 4.7872645467262345 1107 26-27 7.485076898642134 1107 28-29 6.659009127020397 1107 30-31 5.272451735589041 1107 32-33 5.2896440914056875 1107 34-35 6.983362599164945 1107 36-37 6.893645301521296 1107 38-39 6.703131583894137 1107 40-41 7.981595813457595 1107 42-43 6.555026562496675 1107 44-45 4.532162012008591 1107 46-47 3.546036109944776 1107 48-49 4.273978097041432 1107 50-51 5.184024265451306 1107 52-53 4.252649729815442 1107 54-55 5.552155233376855 1107 56-57 5.571357847909436 1107 58-59 3.30962541416395 1107 60-61 3.0353237679674763 1107 62-63 0.646269231245423 1107 64-65 2.41213939739972 1107 66-67 4.411086886936548 1107 68-69 5.258516498572764 1107 70-71 4.419097931502872 1107 72-73 2.5782999362457026 1107 74-75 2.7321513298994766 1107 76-77 3.3172539469150912 1107 78-79 4.051327531755778 1107 80-81 2.2280317142986696 1107 82-83 1.8221953986985682 1107 84-85 2.4630050470898652 1107 86-87 2.436784452839497 1107 88-89 2.6641640409223655 1107 90-91 1.535375891482623 1107 92-93 2.3686799819872704 1107 94-95 2.3290874847477525 1107 96-97 2.4081455748930445 1107 98-99 1.872231545067514 1107 100 0.031758488469836976 1108 1 4.495242817736006 1108 2 -3.3651096092153026 1108 3 -0.11334287477540528 1108 4 7.000389979108128 1108 5 7.222434186079923 1108 6 7.0380965056319305 1108 7 4.380849638808893 1108 8 5.326792568756584 1108 9 5.029773666584646 1108 10-11 3.2655665752991823 1108 12-13 2.5921173873965806 1108 14-15 4.096715959737615 1108 16-17 4.717919145984268 1108 18-19 4.118830086584598 1108 20-21 4.855478823185422 1108 22-23 4.2374815580364675 1108 24-25 3.0534404992917707 1108 26-27 4.832144141412996 1108 28-29 6.190790945761812 1108 30-31 4.675259056187368 1108 32-33 4.711616793097228 1108 34-35 3.0919898212458357 1108 36-37 1.1943889647280272 1108 38-39 2.521083485098419 1108 40-41 5.529593103817902 1108 42-43 3.1453521371452133 1108 44-45 3.993678214763616 1108 46-47 4.924020253314314 1108 48-49 5.034603539101916 1108 50-51 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2213 48-49 -6.435204943294586 2213 50-51 -24.46383592444824 2213 52-53 -18.809760374683066 2213 54-55 -18.72038795172909 2213 56-57 -18.61073012970627 2213 58-59 -19.031942571520833 2213 60-61 -20.458703634346612 2213 62-63 -18.533150894989294 2213 64-65 -18.65224467199041 2213 66-67 -9.260542361598464 2213 68-69 -23.884802615229358 2213 70-71 -18.466287270813485 2213 72-73 -10.085365314386483 2213 74-75 -4.6798445438224014 2213 76-77 -3.451084464117649 2213 78-79 -4.93919709644269 2213 80-81 -3.525757694592013 2213 82-83 -3.5899338910203085 2213 84-85 -3.2743510374934104 2213 86-87 -3.4210039340994456 2213 88-89 -3.0609749361295933 2213 90-91 -3.1150189064279026 2213 92-93 -3.983726610785183 2213 94-95 -3.9486122656336278 2213 96-97 -3.6831821633531554 2213 98-99 -3.919060484054164 2213 100 -3.459136227319565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 889.0 3 710.0 4 3034.0 5 12247.0 6 21988.0 7 26476.0 8 23079.0 9 22837.0 10 26260.0 11 32758.0 12 40108.0 13 55775.0 14 38033.0 15 41978.0 16 50337.0 17 55842.0 18 56570.0 19 58112.0 20 57511.0 21 55513.0 22 58211.0 23 65921.0 24 74471.0 25 83583.0 26 93672.0 27 102980.0 28 113003.0 29 118719.0 30 125518.0 31 138584.0 32 158433.0 33 195518.0 34 241334.0 35 291971.0 36 330815.0 37 302737.0 38 102132.0 39 382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.32070552848368 21.810153087920398 14.25770824926472 23.611433134331207 2 14.29670080435463 21.75610772857663 41.415558735776855 22.531632731291886 3 16.913068174826112 27.75031947133243 30.701826540505984 24.634785813335476 4 11.571819335507534 18.043897064829554 41.12035732856258 29.26392627110034 5 12.437721758482887 38.25694922862007 36.51779643852359 12.787532574373447 6 28.992756956780152 39.47196636702787 19.290168332756892 12.245108343435088 7 24.637995337841275 33.324851473827735 25.200540900297614 16.836612288033372 8 22.894106442938682 37.0341777926705 22.76995484821794 17.301760916172885 9 24.001825937963115 17.24183712504782 23.144728086333433 35.611608850655635 10-11 22.369393378555436 28.02547666682943 30.040645673233872 19.564484281381265 12-13 23.096072473243915 25.84484605586978 30.14778859570838 20.911292875177928 14-15 20.86456525655046 27.19100861845229 27.328502905245582 24.615923219751668 16-17 20.232995990139866 30.860814174444485 28.207653318205807 20.698536517209842 18-19 20.090518571491547 29.956308135405596 29.841347590334887 20.11182570276797 20-21 21.84581844727082 29.335221735907087 28.511966358254327 20.306993458567767 22-23 20.68328240728476 29.393203585521192 29.08939185316757 20.834122154026474 24-25 21.02610114848269 29.066316132222237 28.622713184838585 21.28486953445649 26-27 21.010375406831713 29.030269927396706 28.72789352906772 21.231461136703864 28-29 20.69267189648432 28.929845936058605 29.269467768180352 21.108014399276723 30-31 21.52671009517878 29.426263789921776 28.37224912026138 20.674776994638062 32-33 20.473259710634707 29.23223504117136 29.17678286662893 21.117722381564995 34-35 20.670069116179214 28.503771052758708 29.06518341047936 21.76097642058272 36-37 21.36558660998746 29.280056581021107 28.470697278936612 20.88365953005482 38-39 21.02241358309173 28.872772719030216 28.833205730063522 21.271607967814536 40-41 21.26640202459311 28.898387160155988 28.45488148891095 21.380329326339957 42-43 20.868003385420646 28.998247787950156 29.01569187339723 21.118056953231967 44-45 21.376606908337312 28.522157683940186 28.803505017490536 21.297730390231965 46-47 21.15878401066754 29.10218391632326 28.9428181429065 20.796213930102702 48-49 20.963730822764816 28.657421650611774 29.06424629239856 21.314601234224853 50-51 21.335352643433854 28.777757374128477 28.645111947660734 21.241778034776935 52-53 20.66888221433192 28.908051052390455 28.87358721375646 21.549479519521167 54-55 21.188851003417373 28.89986372751648 28.479876925912954 21.431408343153187 56-57 21.33531650567378 28.797037070190555 28.480267648342306 21.387378775793362 58-59 20.885478301781156 28.234130427950276 29.08509082822906 21.795300442039505 60-61 21.522349108473847 28.804200985916502 28.36311970220779 21.310330203401865 62-63 20.828387182094986 28.94570621593453 28.89494847081272 21.33095813115776 64-65 20.858590140983353 28.293415464179112 28.926514484040716 21.921479910796823 66-67 21.075554616779378 28.630345492686153 28.593544981254205 21.700554909280264 68-69 21.519614839952336 28.49443413580356 28.54865740866963 21.437293615574475 70-71 21.585347802788718 28.58449717268541 28.295754468930767 21.53440055559511 72-73 21.170782729535052 28.713149654305216 28.839621090400836 21.2764465257589 74-75 21.447760914077534 28.19017124963063 28.45152119226863 21.910546644023203 76-77 21.35350010652116 28.695598286658775 28.574601963874063 21.376299642946 78-79 21.295766730157727 28.41189983459439 28.845354674271324 21.446978760976563 80-81 21.519413117239797 28.382844137310002 28.469299598745224 21.628443146704974 82-83 20.838492892756523 28.68700694732723 28.961192992087486 21.513307167828756 84-85 21.11798414858476 28.193764826078993 28.479156961353784 22.209094063982462 86-87 21.367915457066175 28.546142571578088 28.49954374214191 21.586398229213835 88-89 21.111964804607485 28.007996717719248 28.93994317474611 21.940095302927162 90-91 21.45497607774858 28.03619305103092 28.533982176801683 21.974848694418814 92-93 20.87103625785403 28.40685186828792 28.927444505314575 21.79466736854347 94-95 20.898431151026937 27.903695403240004 28.78619211729905 22.411681328434018 96-97 21.151185722275095 28.172003316293992 28.781590030513833 21.895220930917084 98-99 21.079547596664753 27.9025019869811 28.790290965144077 22.227659451210073 100 21.286144659497154 27.766884251329888 28.486255167023184 22.460715922149777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 391.0 1 474.0 2 472.0 3 362.5 4 404.5 5 527.0 6 758.0 7 1119.0 8 1596.0 9 2351.0 10 3337.0 11 4452.5 12 5626.5 13 6805.0 14 7870.5 15 8866.5 16 9752.0 17 10658.5 18 11617.0 19 12552.5 20 13714.5 21 14871.5 22 16125.0 23 18084.5 24 20665.5 25 24138.0 26 29346.5 27 36287.5 28 45034.0 29 55407.5 30 66582.0 31 77897.0 32 89482.5 33 100645.0 34 110645.0 35 119442.5 36 126874.5 37 132695.0 38 136532.5 39 137859.0 40 136806.5 41 133200.5 42 127729.0 43 121433.0 44 114583.0 45 107896.5 46 101549.0 47 95841.0 48 91112.5 49 88307.5 50 87180.5 51 81546.0 52 71504.5 53 63447.5 54 57150.5 55 51501.0 56 46411.0 57 41638.5 58 36862.5 59 32070.5 60 27442.5 61 22723.0 62 18297.5 63 14488.5 64 11112.0 65 8433.0 66 6501.0 67 5024.5 68 3698.5 69 2714.5 70 2104.5 71 1556.5 72 1130.5 73 831.5 74 612.5 75 458.5 76 314.5 77 212.0 78 146.5 79 100.5 80 62.0 81 41.0 82 39.0 83 31.5 84 17.5 85 10.0 86 7.5 87 6.5 88 6.0 89 4.5 90 2.5 91 1.5 92 1.0 93 0.0 94 0.5 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 2.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.24121510377692 2 23.601687715315336 3 5.8000189747474185 4 23.437961880281545 5 12.191061673725253 6 9.152143002482276 7 7.6867555957963924 8 8.323385827083921 9 5.10399351319889 10-11 6.599597137436658 12-13 9.741229594138694 14-15 7.024866375984925 16-17 7.425059662157977 18-19 6.579722462287689 20-21 7.478323181436718 22-23 5.573542246726017 24-25 5.1403719477578225 26-27 9.297016114197474 28-29 6.129941632822774 30-31 4.1938920227050245 32-33 8.540527711520387 34-35 6.8592339144019245 36-37 6.790625864655141 38-39 5.707082370232709 40-41 8.008487386216341 42-43 6.17747001944149 44-45 5.960099339819117 46-47 5.744086178299783 48-49 3.7183793613319667 50-51 6.305442793424487 52-53 4.314162025429212 54-55 4.680203816852809 56-57 4.577612055492899 58-59 3.421174414841059 60-61 3.972143728525665 62-63 3.1168310585499084 64-65 4.299305591357765 66-67 2.224575592556652 68-69 4.892434231298511 70-71 2.3095806306266455 72-73 1.4782609491461516 74-75 0.7897399696953149 76-77 1.0541051805026234 78-79 1.6702811221702232 80-81 1.9599358275262575 82-83 0.755802017119371 84-85 1.1519837610328851 86-87 0.46013762488022575 88-89 0.7384288360029664 90-91 0.33234788704595214 92-93 0.32369942901873405 94-95 0.5240629998221499 96-97 0.5054543247018569 98-99 0.4744907095426811 100 0.39978145483842337 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3278041.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.08317497071968 #Duplication Level Percentage of deduplicated Percentage of total 1 94.67665003219635 87.1812653055633 2 4.1276617679456 7.601764015953696 3 0.7620161483485905 2.1050659895669144 4 0.20942596311076542 0.7713843041816039 5 0.08141087635211794 0.37482859858258505 6 0.04586152133525647 0.2533844696122689 7 0.02239845043367216 0.1443764301249778 8 0.014063044806821443 0.10359758524540877 9 0.00958514285298918 0.07943673478060313 >10 0.04798101076077096 0.7176840143946353 >50 0.0015715325130661703 0.09709304625953649 >100 0.0010409675794252097 0.21522403578633983 >500 2.3347923565063245E-4 0.15528788415946151 >1k 1.0006252888883943E-4 0.1996075857886272 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.050602478736538E-5 0.0 0.0 0.0 3 0.0 3.050602478736538E-5 0.0 0.0 0.0 4 0.0 3.050602478736538E-5 0.0 3.050602478736538E-5 0.0 5 0.0 3.050602478736538E-5 0.0 3.050602478736538E-5 0.0 6 0.0 3.050602478736538E-5 0.0 3.050602478736538E-5 0.0 7 0.0 3.050602478736538E-5 0.0 3.050602478736538E-5 0.0 8 0.0 3.050602478736538E-5 0.0 3.050602478736538E-5 0.0 9 0.0 3.050602478736538E-5 0.0 6.101204957473076E-5 0.0 10-11 0.0 3.050602478736538E-5 0.0 7.626506196841345E-5 0.0 12-13 0.0 3.050602478736538E-5 0.0 9.151807436209614E-5 0.0 14-15 0.0 3.050602478736538E-5 0.0 9.151807436209614E-5 0.0 16-17 0.0 3.050602478736538E-5 0.0 9.151807436209614E-5 0.0 18-19 0.0 3.050602478736538E-5 0.0 9.151807436209614E-5 0.0 20-21 0.0 3.050602478736538E-5 0.0 1.0677108675577883E-4 0.0 22-23 0.0 3.050602478736538E-5 0.0 1.677831363305096E-4 0.0 24-25 0.0 3.050602478736538E-5 0.0 2.1354217351155766E-4 0.0 26-27 0.0 3.050602478736538E-5 0.0 2.4404819829892304E-4 0.0 28-29 0.0 3.050602478736538E-5 0.0 7.016385701094038E-4 0.0 30-31 0.0 3.050602478736538E-5 0.0 0.0025930121069260574 0.0 32-33 0.0 3.050602478736538E-5 0.0 0.007412964023329788 0.0 34-35 0.0 3.050602478736538E-5 0.0 0.014078530439369123 0.0 36-37 0.0 3.050602478736538E-5 0.0 0.025640313833780605 0.0 38-39 0.0 3.050602478736538E-5 0.0 0.04713180829647951 0.0 40-41 0.0 3.050602478736538E-5 0.0 0.07634132703038186 0.0 42-43 0.0 3.050602478736538E-5 0.0 0.10474243610741904 0.0 44-45 0.0 3.050602478736538E-5 0.0 0.13945829231544082 0.0 46-47 0.0 3.050602478736538E-5 0.0 0.1820599559309966 0.0 48-49 0.0 3.050602478736538E-5 0.0 0.22788000516161938 0.0 50-51 0.0 3.050602478736538E-5 0.0 0.2772235002551829 1.525301239368269E-5 52-53 0.0 6.101204957473076E-5 0.0 0.3296175978274829 3.050602478736538E-5 54-55 0.0 6.101204957473076E-5 0.0 0.38882979193975914 3.050602478736538E-5 56-57 0.0 6.101204957473076E-5 0.0 0.45801745615750383 3.050602478736538E-5 58-59 0.0 6.101204957473076E-5 0.0 0.5345265663242162 3.050602478736538E-5 60-61 0.0 6.101204957473076E-5 0.0 0.6173199175971258 3.050602478736538E-5 62-63 0.0 6.101204957473076E-5 0.0 0.7051467629599508 3.050602478736538E-5 64-65 0.0 6.101204957473076E-5 0.0 0.8063047411548543 3.050602478736538E-5 66-67 0.0 6.101204957473076E-5 0.0 0.9263917077303182 3.050602478736538E-5 68-69 0.0 6.101204957473076E-5 0.0 1.0604961926955765 3.050602478736538E-5 70-71 0.0 6.101204957473076E-5 0.0 1.2206223168044574 3.050602478736538E-5 72-73 0.0 6.101204957473076E-5 0.0 1.3886647543456596 3.050602478736538E-5 74-75 0.0 6.101204957473076E-5 3.050602478736538E-5 1.562991432993059 3.050602478736538E-5 76-77 0.0 6.101204957473076E-5 3.050602478736538E-5 1.7594807386484794 3.050602478736538E-5 78-79 0.0 6.101204957473076E-5 3.050602478736538E-5 1.9799020207495879 3.050602478736538E-5 80-81 3.050602478736538E-5 6.101204957473076E-5 3.050602478736538E-5 2.2257043154737843 3.050602478736538E-5 82-83 3.050602478736538E-5 6.101204957473076E-5 3.050602478736538E-5 2.4944013818008988 3.050602478736538E-5 84-85 3.050602478736538E-5 6.101204957473076E-5 3.050602478736538E-5 2.7674150506354254 3.050602478736538E-5 86-87 3.050602478736538E-5 6.101204957473076E-5 3.050602478736538E-5 3.0682807201008164 3.050602478736538E-5 88 3.050602478736538E-5 6.101204957473076E-5 3.050602478736538E-5 3.3204587740055724 3.050602478736538E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 200 9.328036E-4 18.47407 1 GTACATA 1480 0.0 17.891554 1 GTACATG 12780 0.0 16.286478 1 GTATATA 2030 0.0 15.774247 1 TACATGG 12995 0.0 15.537621 2 GTATAAG 1320 0.0 15.395059 1 ACATGGG 13250 0.0 14.801848 3 GTTACAC 875 0.0 14.687385 3 GTATAGG 815 0.0 14.356128 1 GTATTAA 2070 0.0 14.279474 1 TAGACTG 1375 0.0 14.163728 5 CATGGGG 4440 0.0 13.970953 4 CTATACA 1330 0.0 13.865908 4 GTATCAA 9755 0.0 13.824778 1 TACACTG 2160 0.0 13.639986 5 GTATTAC 1175 0.0 13.626264 1 CTAGACT 1110 0.0 13.593359 4 CTATACT 1370 0.0 13.461064 4 CCTAGAC 955 0.0 13.457027 3 CCTATAC 1060 0.0 13.178284 3 >>END_MODULE