FastQCFastQC Report
Wed 25 May 2016
SRR1295151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278041
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT78740.240204439175715No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA62590.1909372091441199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134150.019.3075331
GAGTACT50800.017.3294312-13
CAACGCA146100.017.2558925
TCAACGC148450.017.0460974
AACGCAG148000.017.0057666
ATCAACG149750.016.9920853
AGTACTT59950.016.87737312-13
TATCAAC151800.016.786232
CATGGGG48350.016.3431454
GTACATG162200.016.0909541
TACATGG164850.015.4874582
ACGCAGA161650.015.4812097
CGCAGAG160750.015.4802618
TAGGACG3051.931916E-615.4213724
GTATATA24900.015.343991
ACATGGG159300.015.2647963
GTACTTT63000.014.15977914-15
TACCGAC2000.00570323914.0855767
CATGGGT24050.014.081224
GCAGAGT173750.013.9975389
Produced by FastQC (version 0.11.5)