##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295151_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3278041 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55055961777171 33.0 33.0 33.0 27.0 33.0 2 30.290336515010033 33.0 33.0 33.0 27.0 33.0 3 31.047285558661407 33.0 33.0 33.0 27.0 33.0 4 35.29162081865358 37.0 37.0 37.0 33.0 37.0 5 35.591355324719856 37.0 37.0 37.0 33.0 37.0 6 35.41796213043095 37.0 37.0 37.0 33.0 37.0 7 35.328155749119674 37.0 37.0 37.0 33.0 37.0 8 35.33886397394053 37.0 37.0 37.0 33.0 37.0 9 35.29024286151393 37.0 37.0 37.0 33.0 37.0 10-11 35.27334038836 37.0 37.0 37.0 33.0 37.0 12-13 35.205238738624686 37.0 37.0 37.0 33.0 37.0 14-15 36.72308110240232 40.0 37.0 40.0 33.0 40.0 16-17 36.72917940928744 40.0 37.0 40.0 33.0 40.0 18-19 36.76780400245147 40.0 37.0 40.0 33.0 40.0 20-21 36.763466808377316 40.0 37.0 40.0 33.0 40.0 22-23 36.715648614523126 40.0 37.0 40.0 33.0 40.0 24-25 36.69016205105427 40.0 37.0 40.0 33.0 40.0 26-27 36.669225613712584 40.0 37.0 40.0 33.0 40.0 28-29 36.61859308654162 40.0 37.0 40.0 33.0 40.0 30-31 36.51756292859058 40.0 37.0 40.0 33.0 40.0 32-33 36.47253146010071 40.0 37.0 40.0 33.0 40.0 34-35 36.39088513535981 40.0 37.0 40.0 33.0 40.0 36-37 36.37760464252888 40.0 37.0 40.0 33.0 40.0 38-39 36.30923148917296 37.0 37.0 40.0 33.0 40.0 40-41 36.24142849342031 37.0 37.0 40.0 30.0 40.0 42-43 36.14670606621455 37.0 37.0 40.0 27.0 40.0 44-45 36.03945420450812 37.0 37.0 40.0 27.0 40.0 46-47 35.92719172823037 37.0 37.0 40.0 27.0 40.0 48-49 35.82239849959168 37.0 37.0 40.0 27.0 40.0 50-51 35.72107655151354 37.0 37.0 40.0 27.0 40.0 52-53 35.6350944054696 37.0 37.0 40.0 27.0 40.0 54-55 35.52049089684967 37.0 37.0 40.0 27.0 40.0 56-57 35.40122591511211 37.0 35.0 40.0 27.0 40.0 58-59 35.28348120111981 37.0 33.0 40.0 27.0 40.0 60-61 35.12533400283889 37.0 33.0 40.0 27.0 40.0 62-63 34.95561281875364 37.0 33.0 40.0 27.0 40.0 64-65 34.779784175975834 37.0 33.0 40.0 27.0 40.0 66-67 34.55761108540131 37.0 33.0 40.0 27.0 40.0 68-69 34.341848225815355 37.0 33.0 38.5 27.0 40.0 70-71 34.1121123866358 37.0 33.0 37.0 27.0 40.0 72-73 33.89127042645288 37.0 33.0 37.0 27.0 40.0 74-75 33.65300296732103 37.0 33.0 37.0 27.0 40.0 76-77 33.40454161494624 37.0 33.0 37.0 27.0 38.5 78-79 33.16341040273748 37.0 33.0 37.0 24.5 37.0 80-81 32.95117068395423 37.0 33.0 37.0 22.0 37.0 82-83 32.73580394510014 37.0 33.0 37.0 22.0 37.0 84-85 32.558369770237775 37.0 33.0 37.0 22.0 37.0 86-87 32.573896269143674 37.0 33.0 37.0 22.0 37.0 88-89 32.582158978487456 37.0 33.0 37.0 22.0 37.0 90-91 32.59039880831265 37.0 33.0 37.0 22.0 37.0 92-93 32.55392519495638 37.0 33.0 37.0 22.0 37.0 94-95 32.57901121431977 37.0 33.0 37.0 22.0 37.0 96-97 32.53572713092972 37.0 33.0 37.0 22.0 37.0 98-99 32.461694194795 33.0 33.0 37.0 22.0 37.0 100 31.938315292578707 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5683273501255677 1101 2 4.114724463203526 1101 3 2.3621320094978167 1101 4 1.103921727341394 1101 5 0.4715391264721589 1101 6 0.4932652566687352 1101 7 0.49677339894741834 1101 8 0.4990313076113182 1101 9 0.5482193244923224 1101 10-11 0.5497763239593922 1101 12-13 0.6440742645014623 1101 14-15 0.8358937838809624 1101 16-17 0.9019489750902423 1101 18-19 0.8618259093536054 1101 20-21 0.9021294772330748 1101 22-23 0.8377453365419072 1101 24-25 0.8024873482296329 1101 26-27 0.8788354448114788 1101 28-29 0.7501941742447613 1101 30-31 0.6101617450966756 1101 32-33 0.9098518593534095 1101 34-35 0.9878637721045962 1101 36-37 0.8961603378201772 1101 38-39 0.9047180648040083 1101 40-41 0.9015093742613871 1101 42-43 0.8919194786782967 1101 44-45 0.9541181996679384 1101 46-47 0.9837871542214245 1101 48-49 0.8980251643327861 1101 50-51 0.9753375171599785 1101 52-53 0.9294714973251388 1101 54-55 0.9070269431528075 1101 56-57 0.8476648137558769 1101 58-59 0.8307799248361363 1101 60-61 0.841196264757464 1101 62-63 0.9429204815673131 1101 64-65 0.8473392615489672 1101 66-67 0.8893243318714141 1101 68-69 0.8805217373703655 1101 70-71 0.7650906945111586 1101 72-73 0.769547186889902 1101 74-75 0.7661242285483212 1101 76-77 0.7379838553529368 1101 78-79 0.8955734530464667 1101 80-81 0.7936149664279526 1101 82-83 0.8307049980370138 1101 84-85 0.8571277954718752 1101 86-87 0.8743610506701458 1101 88-89 0.7781389084492005 1101 90-91 0.7800556331814761 1101 92-93 0.8210669465266491 1101 94-95 0.8096288602250752 1101 96-97 0.7437150366372833 1101 98-99 0.7763979999789754 1101 100 0.8424444966438358 1102 1 0.43675099841825116 1102 2 3.930132313630903 1102 3 2.197726988157818 1102 4 0.9604236385419327 1102 5 0.3611073599287309 1102 6 0.3840708387203904 1102 7 0.3009627226818168 1102 8 0.33403551991123237 1102 9 0.3828184365441416 1102 10-11 0.4166614964316082 1102 12-13 0.5064510576299526 1102 14-15 0.7070251403343661 1102 16-17 0.6526451649895435 1102 18-19 0.6808431670187147 1102 20-21 0.7209524817285171 1102 22-23 0.6695012041712545 1102 24-25 0.7231091672469532 1102 26-27 0.6671011144511212 1102 28-29 0.5872403076319443 1102 30-31 0.6343615241540022 1102 32-33 0.7304771596103166 1102 34-35 0.7719202169234691 1102 36-37 0.6324056120374166 1102 38-39 0.626278309976108 1102 40-41 0.6550147504557344 1102 42-43 0.7010557019046573 1102 44-45 0.7980694298219717 1102 46-47 0.6697250349820081 1102 48-49 0.6501936865001525 1102 50-51 0.6369464648740788 1102 52-53 0.6362990204695862 1102 54-55 0.622406827622072 1102 56-57 0.6299202392677969 1102 58-59 0.6052894761971785 1102 60-61 0.5910831824911327 1102 62-63 0.6949046720978771 1102 64-65 0.6572843507963526 1102 66-67 0.6046155027515425 1102 68-69 0.6006147560924688 1102 70-71 0.6282049882488678 1102 72-73 0.5846214765429636 1102 74-75 0.5438520118573962 1102 76-77 0.5956791935089498 1102 78-79 0.5601247444703148 1102 80-81 0.5681741834739569 1102 82-83 0.5865396136269396 1102 84-85 0.5270298682019359 1102 86-87 0.5604665531174575 1102 88-89 0.5637910374592963 1102 90-91 0.6091459156492576 1102 92-93 0.6523937513753211 1102 94-95 0.5851943295773907 1102 96-97 0.619844814928733 1102 98-99 0.6899008580766832 1102 100 0.6081047369422024 1103 1 0.4634878936818332 1103 2 3.9584809740723337 1103 3 2.2497897172090013 1103 4 0.9749613828730261 1103 5 0.3786704087371291 1103 6 0.3530286661647679 1103 7 0.3316091514302357 1103 8 0.2764822880034714 1103 9 0.3990249359931184 1103 10-11 0.4272128357541902 1103 12-13 0.4587159062821442 1103 14-15 0.6251152575944161 1103 16-17 0.6231014856812536 1103 18-19 0.7247556266950852 1103 20-21 0.6875462885857146 1103 22-23 0.6546330833761758 1103 24-25 0.6272338584039545 1103 26-27 0.5659780704567723 1103 28-29 0.5624664385223781 1103 30-31 0.5473680325703398 1103 32-33 0.6491763494800367 1103 34-35 0.7524996264463297 1103 36-37 0.58435164740186 1103 38-39 0.5468934473537246 1103 40-41 0.605652023056436 1103 42-43 0.6316062624827268 1103 44-45 0.6675386918317443 1103 46-47 0.6929851309327688 1103 48-49 0.6189537010367232 1103 50-51 0.5348404919036014 1103 52-53 0.5512837759556035 1103 54-55 0.5297264361731564 1103 56-57 0.49987503668802447 1103 58-59 0.5223134804071279 1103 60-61 0.5321985334335295 1103 62-63 0.5915633271352974 1103 64-65 0.5443772502981901 1103 66-67 0.5243296563152384 1103 68-69 0.5466924205196619 1103 70-71 0.5041663247632613 1103 72-73 0.5609592062430977 1103 74-75 0.5238336759636439 1103 76-77 0.5087677060833187 1103 78-79 0.4602226184977738 1103 80-81 0.48682051310943564 1103 82-83 0.5368344746210383 1103 84-85 0.5836157460239946 1103 86-87 0.5680272774914599 1103 88-89 0.5300892753728235 1103 90-91 0.5213698448165403 1103 92-93 0.5532109611572977 1103 94-95 0.4377164589009013 1103 96-97 0.4447041099525606 1103 98-99 0.5110213534525911 1103 100 0.6073552543353458 1104 1 0.405940536566483 1104 2 3.6413216594498152 1104 3 2.1354038945622626 1104 4 0.8898860205333818 1104 5 0.2853220531694589 1104 6 0.2360614352074819 1104 7 0.22979619738439538 1104 8 0.22059407447724055 1104 9 0.3403366784527009 1104 10-11 0.35176515881834547 1104 12-13 0.30248311427549623 1104 14-15 0.4069912189097664 1104 16-17 0.4695665960635793 1104 18-19 0.48314607083175076 1104 20-21 0.49765442236014934 1104 22-23 0.4484666714655532 1104 24-25 0.49416407199237966 1104 26-27 0.4091922305870952 1104 28-29 0.42314346766025324 1104 30-31 0.35647161422503615 1104 32-33 0.4812744150085493 1104 34-35 0.549299866610717 1104 36-37 0.4153159887319049 1104 38-39 0.3949198654359023 1104 40-41 0.3829395050859645 1104 42-43 0.43710295304622804 1104 44-45 0.4757348396333114 1104 46-47 0.46777355185194835 1104 48-49 0.3842460605082678 1104 50-51 0.3918846161288556 1104 52-53 0.3075269136889176 1104 54-55 0.34023147508026597 1104 56-57 0.3615059657462396 1104 58-59 0.38665762113770796 1104 60-61 0.32820161442681695 1104 62-63 0.3807484546448876 1104 64-65 0.3210099016507826 1104 66-67 0.37384669626529643 1104 68-69 0.36921650274008044 1104 70-71 0.3258364958205817 1104 72-73 0.3653491432597491 1104 74-75 0.37986086907829986 1104 76-77 0.3598773301208027 1104 78-79 0.42388406087147246 1104 80-81 0.3772330743764627 1104 82-83 0.41471662662786457 1104 84-85 0.3316427023231654 1104 86-87 0.40285021606056404 1104 88-89 0.4349642655055206 1104 90-91 0.39838295578253025 1104 92-93 0.4322420270422356 1104 94-95 0.4346521503422949 1104 96-97 0.35737086751809244 1104 98-99 0.3436248645410558 1104 100 0.3822114307939728 1105 1 0.5309972307499926 1105 2 3.7103787331759754 1105 3 2.1910817340157216 1105 4 0.9181733343202723 1105 5 0.311672276150766 1105 6 0.2594257249882972 1105 7 0.2615461262201464 1105 8 0.25347575205576334 1105 9 0.3129243290836925 1105 10-11 0.24119276105764698 1105 12-13 0.29810491188437993 1105 14-15 0.43207720904165825 1105 16-17 0.42351348381384213 1105 18-19 0.49320217197986693 1105 20-21 0.387606979528762 1105 22-23 0.33813273323612236 1105 24-25 0.419097770635517 1105 26-27 0.3810714411383813 1105 28-29 0.31936944972685666 1105 30-31 0.3093043070801471 1105 32-33 0.3310169190411898 1105 34-35 0.5239054387712585 1105 36-37 0.39516630659886687 1105 38-39 0.3607689142071351 1105 40-41 0.3864106598444792 1105 42-43 0.5056947312035476 1105 44-45 0.5127516936991654 1105 46-47 0.40428531767413745 1105 48-49 0.37041611933737784 1105 50-51 0.3484521865814685 1105 52-53 0.27233536325719143 1105 54-55 0.2704348862198387 1105 56-57 0.32469821595973514 1105 58-59 0.28849170169564076 1105 60-61 0.3344779451982376 1105 62-63 0.3651989480503346 1105 64-65 0.23767615319268032 1105 66-67 0.2779658607970319 1105 68-69 0.27149108998744964 1105 70-71 0.22300736117782094 1105 72-73 0.343657035374747 1105 74-75 0.24199544066952683 1105 76-77 0.2073169591178612 1105 78-79 0.22283699758273912 1105 80-81 0.23767660554640457 1105 82-83 0.24107058573214601 1105 84-85 0.2667891820650823 1105 86-87 0.3305261103579298 1105 88-89 0.3018837431599408 1105 90-91 0.21637392978386316 1105 92-93 0.2275258537810103 1105 94-95 0.3028611834573951 1105 96-97 0.21944850771166102 1105 98-99 0.23839180163186313 1105 100 0.22525441399920965 1106 1 0.4363533669082109 1106 2 3.818589473559264 1106 3 2.1550270554918924 1106 4 0.8050299606970981 1106 5 0.2786619509213537 1106 6 0.14641449974816823 1106 7 0.08121327544575507 1106 8 0.16055297680567548 1106 9 0.19454069089566417 1106 10-11 0.11991141387563431 1106 12-13 0.1420455513898773 1106 14-15 0.24577853186593757 1106 16-17 0.21573488262199447 1106 18-19 0.2258979338868201 1106 20-21 0.24771421484901168 1106 22-23 0.07497136897305268 1106 24-25 0.13778806354113016 1106 26-27 0.18242865730946534 1106 28-29 0.13710863236392612 1106 30-31 0.042836236396745164 1106 32-33 0.21026023981266917 1106 34-35 0.29627523402839273 1106 36-37 0.137002979546466 1106 38-39 0.14194740165294917 1106 40-41 0.16299663193266412 1106 42-43 0.20628082967620998 1106 44-45 0.13217929645357884 1106 46-47 0.1240741789882236 1106 48-49 0.01200559572262705 1106 50-51 0.10253311286484035 1106 52-53 0.060746691136841946 1106 54-55 0.13833757399655155 1106 56-57 0.12304967397710698 1106 58-59 0.04550756294845826 1106 60-61 0.11110043474278797 1106 62-63 0.16877147359531364 1106 64-65 0.10428691387408406 1106 66-67 0.10101497265879544 1106 68-69 0.06519096704981564 1106 70-71 0.09232080618907901 1106 72-73 0.051116046588504105 1106 74-75 0.0028846704135432333 1106 76-77 0.054797331224420986 1106 78-79 0.10557754938506037 1106 80-81 0.10094344340659944 1106 82-83 0.12935940683298952 1106 84-85 0.13377523764361854 1106 86-87 0.22974783087204997 1106 88-89 0.08796412943689091 1106 90-91 0.11037169124655577 1106 92-93 0.22800896804790938 1106 94-95 0.18380140742601725 1106 96-97 0.12420234520419626 1106 98-99 0.14956769436590633 1106 100 0.20644702395371084 1107 1 0.3510722655343308 1107 2 3.499321556899659 1107 3 2.0556963732524522 1107 4 0.8790506641063658 1107 5 0.2620906820342839 1107 6 0.14879140775514088 1107 7 0.10528551890855908 1107 8 0.0535907314909565 1107 9 0.10047350496247276 1107 10-11 0.13011349099358682 1107 12-13 0.16357533181925277 1107 14-15 0.006267517590202942 1107 16-17 0.052782993622265906 1107 18-19 0.07482791926974386 1107 20-21 0.11459320340227919 1107 22-23 0.06808792451175094 1107 24-25 0.06188791405401162 1107 26-27 0.07679036840471554 1107 28-29 0.056745630865343344 1107 30-31 0.026882801185919902 1107 32-33 0.0898625950805183 1107 34-35 0.2681158247627238 1107 36-37 0.1105085309616669 1107 38-39 -0.014041310476748947 1107 40-41 -0.030521323397870503 1107 42-43 0.027317458754275492 1107 44-45 0.11374705132801921 1107 46-47 0.037660583334726994 1107 48-49 0.0020983625066506306 1107 50-51 0.06374405966068508 1107 52-53 -0.01729318971438687 1107 54-55 -0.05185512723519281 1107 56-57 -0.07986599554432416 1107 58-59 -0.005730266333351608 1107 60-61 0.03782280323227383 1107 62-63 0.030219608417596078 1107 64-65 -0.03750198274909167 1107 66-67 -0.06992875986257019 1107 68-69 -0.156825988357582 1107 70-71 -0.10471802985928491 1107 72-73 -0.10684182362254546 1107 74-75 -0.033411134716921254 1107 76-77 0.004001725107187326 1107 78-79 -0.01631036568805655 1107 80-81 -0.05364710093495262 1107 82-83 -0.04897580931088896 1107 84-85 -0.007606468746047312 1107 86-87 -0.07480921447426425 1107 88-89 -0.06686760425531446 1107 90-91 -0.012125785443267034 1107 92-93 0.0569895442845052 1107 94-95 -0.07352422699778316 1107 96-97 -0.06737268168331667 1107 98-99 0.06570585832650977 1107 100 0.05558467407902512 1108 1 0.4215196947509732 1108 2 3.662698317255302 1108 3 2.12647246302458 1108 4 0.9337141995209635 1108 5 0.308770089430233 1108 6 0.18466369726024823 1108 7 0.11890450201779146 1108 8 0.06317995563959755 1108 9 0.12903721910495847 1108 10-11 0.10931771323748052 1108 12-13 0.03752094158644326 1108 14-15 -0.014656004167413528 1108 16-17 -0.025321750849272462 1108 18-19 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0.8403788929559823 2202 62-63 0.8677939164584103 2202 64-65 0.8253473082242735 2202 66-67 0.8460532972279182 2202 68-69 0.7545022434779725 2202 70-71 0.8160615682975632 2202 72-73 0.7176043885878798 2202 74-75 0.7444613445067887 2202 76-77 0.7172370161374317 2202 78-79 0.7277506976906167 2202 80-81 0.763303339249596 2202 82-83 0.808771598985345 2202 84-85 0.7937970029928039 2202 86-87 0.8118192603039986 2202 88-89 0.7695816742741357 2202 90-91 0.7564300825038472 2202 92-93 0.7992227639366618 2202 94-95 0.8023903867244613 2202 96-97 0.7322472825614099 2202 98-99 0.7517395824917728 2202 100 0.664824455608084 2203 1 0.31319357305503104 2203 2 3.8852458829344307 2203 3 2.15493525368446 2203 4 0.8875177097516058 2203 5 0.44130995731018174 2203 6 0.4196060217446771 2203 7 0.33757269626845954 2203 8 0.3199767872524504 2203 9 0.48779406683386384 2203 10-11 0.46034217444618974 2203 12-13 0.4975256330714046 2203 14-15 0.6663465327131632 2203 16-17 0.7511817032755417 2203 18-19 0.7925508607307279 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0.7584151717796033 2203 88-89 0.7063639881564896 2203 90-91 0.669254795898425 2203 92-93 0.828347972483563 2203 94-95 0.7637272980191625 2203 96-97 0.789808238560525 2203 98-99 0.6927146104655435 2203 100 0.6012861600309591 2204 1 0.36521853059389997 2204 2 3.918679783268214 2204 3 2.2239746317026814 2204 4 1.0195980540591663 2204 5 0.4433644856015917 2204 6 0.37023582148325573 2204 7 0.3641482973567989 2204 8 0.37098391155625876 2204 9 0.39526202597598825 2204 10-11 0.34446968740928696 2204 12-13 0.38816726111100053 2204 14-15 0.5428397675772061 2204 16-17 0.6290546715533054 2204 18-19 0.6485510793163272 2204 20-21 0.6016583489058007 2204 22-23 0.5223809062175349 2204 24-25 0.6366596764110213 2204 26-27 0.6169370577128106 2204 28-29 0.5608611305839659 2204 30-31 0.5817821345244525 2204 32-33 0.7875627896244168 2204 34-35 0.7536399889344452 2204 36-37 0.5429735295306131 2204 38-39 0.4894044151295134 2204 40-41 0.4862726578578247 2204 42-43 0.6093553314589784 2204 44-45 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0.5849093625806248 2210 50-51 0.5566179416222212 2210 52-53 0.5175107171825388 2210 54-55 0.5420463190812725 2210 56-57 0.4879444085809226 2210 58-59 0.6523315236604574 2210 60-61 0.5557922952625844 2210 62-63 0.5518296859310681 2210 64-65 0.224512006903538 2210 66-67 0.33447299292916455 2210 68-69 0.500138934260292 2210 70-71 0.6310495104699712 2210 72-73 0.5704237127664271 2210 74-75 0.044794132218221705 2210 76-77 0.4237707343216357 2210 78-79 0.47132048106622904 2210 80-81 0.46916336015816285 2210 82-83 0.5091639469505083 2210 84-85 0.5854812670071681 2210 86-87 0.6704504990323201 2210 88-89 0.6032975676987675 2210 90-91 0.7064696505045802 2210 92-93 0.7806076856128428 2210 94-95 0.700825576421785 2210 96-97 0.5317495587627121 2210 98-99 0.6572511967412566 2210 100 0.5795787383235336 2211 1 0.364820656086156 2211 2 3.9518596248957216 2211 3 2.252687360342687 2211 4 0.9799284652473759 2211 5 0.5128223115364676 2211 6 0.36664175471229044 2211 7 0.25313317505241884 2211 8 0.261345532664393 2211 9 0.1869521068614688 2211 10-11 0.2729248912770359 2211 12-13 0.24154307536633013 2211 14-15 0.17069805122142157 2211 16-17 0.22551366074236512 2211 18-19 0.23802258953117672 2211 20-21 0.225880157164994 2211 22-23 0.14600295616605763 2211 24-25 0.20552116058597392 2211 26-27 0.17125898537050688 2211 28-29 0.2288851079009575 2211 30-31 0.19153626037101645 2211 32-33 0.23766677749837584 2211 34-35 0.34876610756106885 2211 36-37 0.1817710482832311 2211 38-39 0.16886029778816436 2211 40-41 0.1856480979195041 2211 42-43 0.31008875101954203 2211 44-45 0.2986403171952716 2211 46-47 0.33354044860124077 2211 48-49 0.22281969329349494 2211 50-51 0.17690627363030131 2211 52-53 0.20355705469384588 2211 54-55 0.15977228890915995 2211 56-57 0.09774359812534072 2211 58-59 0.232808315283755 2211 60-61 0.12479376260800024 2211 62-63 0.3847698963871977 2211 64-65 0.20838784816790934 2211 66-67 0.22257440644459336 2211 68-69 0.2091569604757666 2211 70-71 0.30177859260032847 2211 72-73 0.33950761923887285 2211 74-75 0.2869184767629491 2211 76-77 0.22566533137546685 2211 78-79 0.2114109537044726 2211 80-81 0.19735415331710726 2211 82-83 -0.17630777590974844 2211 84-85 0.20579749770556077 2211 86-87 0.270403145035921 2211 88-89 0.24534048413658383 2211 90-91 0.3302358370733174 2211 92-93 0.3113802416083331 2211 94-95 0.30335304474543534 2211 96-97 0.19793842278597396 2211 98-99 0.27832014558091345 2211 100 0.24098689807564355 2212 1 0.6686430326605013 2212 2 4.136702262587743 2212 3 2.4587533026431707 2212 4 1.2072658431186625 2212 5 0.6389322057636377 2212 6 0.5682247445961366 2212 7 0.4077942408506132 2212 8 0.36155016130663853 2212 9 0.2747575367257227 2212 10-11 0.4309916549741857 2212 12-13 0.44229703103973605 2212 14-15 0.4455230360904281 2212 16-17 0.4629070232809127 2212 18-19 0.4402641377918357 2212 20-21 0.480575808085824 2212 22-23 0.494821081652411 2212 24-25 0.5691725569603037 2212 26-27 0.4556327784938361 2212 28-29 0.29065809980227897 2212 30-31 0.3671078515748647 2212 32-33 0.45300095624581616 2212 34-35 0.7038517057395879 2212 36-37 0.5710624855832833 2212 38-39 0.4157956236088438 2212 40-41 0.43479175587264507 2212 42-43 0.3902089343206896 2212 44-45 0.5372165629802126 2212 46-47 0.5618280527168693 2212 48-49 0.5674076980201264 2212 50-51 0.28781398899473487 2212 52-53 0.3454606774854412 2212 54-55 0.4085187223183837 2212 56-57 0.33406755985113534 2212 58-59 0.3512000350289739 2212 60-61 0.4479979301152852 2212 62-63 0.6074381385983401 2212 64-65 0.600219202463677 2212 66-67 0.5539622785949163 2212 68-69 0.5204965575590847 2212 70-71 0.6222173913437388 2212 72-73 0.43441589661155433 2212 74-75 0.3575817905941179 2212 76-77 0.47376178063620955 2212 78-79 0.5758523319216025 2212 80-81 0.5414746824694845 2212 82-83 0.3344400553148503 2212 84-85 0.3724597451621179 2212 86-87 0.379595366265427 2212 88-89 0.5727821217421436 2212 90-91 0.5835681856913553 2212 92-93 0.48264965971364404 2212 94-95 0.58343936344734 2212 96-97 0.5362226404416717 2212 98-99 0.5390083174689693 2212 100 0.8695078076643696 2213 1 -0.4289400452356702 2213 2 -26.047121266824025 2213 3 -14.874738158457397 2213 4 -6.121683296504102 2213 5 -1.9467685840642872 2213 6 -1.2372095796712017 2213 7 -1.000326633636483 2213 8 -0.915126398481874 2213 9 -1.0455926960059116 2213 10-11 -1.0155366296233552 2213 12-13 -0.9209654286174143 2213 14-15 -1.1493071995604893 2213 16-17 -1.1500393835696698 2213 18-19 -1.2614797276617153 2213 20-21 -1.3333966047006314 2213 22-23 -1.2550684316384348 2213 24-25 -1.1753511540956652 2213 26-27 -1.165828200438682 2213 28-29 -1.2943872707032469 2213 30-31 -1.3296876284037253 2213 32-33 -1.2962635469086337 2213 34-35 -1.2717240415154762 2213 36-37 -1.262333693401132 2213 38-39 -1.208391032029425 2213 40-41 -1.4058205229512168 2213 42-43 -1.3230283870760218 2213 44-45 -1.2167577149029896 2213 46-47 -1.2709745389566933 2213 48-49 -1.281093737293446 2213 50-51 -1.2270966485439843 2213 52-53 -1.2674182209722034 2213 54-55 -1.375761350339296 2213 56-57 -1.3043183611012026 2213 58-59 -1.2612103592362587 2213 60-61 -1.2912254598196853 2213 62-63 -1.2543306218421435 2213 64-65 -1.0317078073807977 2213 66-67 -1.267730823790778 2213 68-69 -1.406320289940865 2213 70-71 -1.309998911223616 2213 72-73 -1.3089392907901214 2213 74-75 -1.2845681794021573 2213 76-77 -1.3611104602369792 2213 78-79 -1.324170170876041 2213 80-81 -1.3565953478586152 2213 82-83 -1.3029504716155245 2213 84-85 -1.2949805311965719 2213 86-87 -1.258904893226294 2213 88-89 -1.1864742317504522 2213 90-91 -1.1816345827609425 2213 92-93 -1.2092886623480155 2213 94-95 -1.348590429445835 2213 96-97 -1.3488713847614555 2213 98-99 -1.3573922485473489 2213 100 -1.3995842956782099 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 47.0 5 52.0 6 47.0 7 50.0 8 67.0 9 100.0 10 376.0 11 1697.0 12 4721.0 13 8587.0 14 11593.0 15 13373.0 16 15042.0 17 16006.0 18 16698.0 19 18149.0 20 20713.0 21 23590.0 22 26762.0 23 28517.0 24 28599.0 25 30085.0 26 32458.0 27 37143.0 28 43742.0 29 53239.0 30 65205.0 31 80052.0 32 101831.0 33 138535.0 34 192393.0 35 278014.0 36 429006.0 37 751275.0 38 803119.0 39 7146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.377754378953355 21.68293226552713 10.799363620293443 29.13994973522608 2 12.663898829784461 23.31166720100432 37.52140432295775 26.503029646253466 3 15.033521545337598 28.398851631202902 29.48999112579739 27.07763569766211 4 10.445636561772002 18.963106056815672 38.83626596636553 31.754991415046792 5 11.388732526933474 39.11470203726895 35.6145574915916 13.882007944205979 6 28.85514963653485 40.700343853447926 18.02488015494009 12.419626355077142 7 24.78047407955362 34.0266244796044 24.266075120070166 16.926826320771816 8 23.664786573688485 37.344439117124374 21.664823807281365 17.32595050190578 9 24.453609531093306 16.838567992172578 22.861513126372195 35.84630935036192 10-11 22.50409032936574 28.142305312987638 29.697820718903046 19.655783638743575 12-13 23.237909830374605 25.8882055097658 30.185925374104546 20.68795928575505 14-15 21.103946986324342 27.076851426415004 27.355693276711435 24.46350831054922 16-17 20.080594279820225 30.763501868758887 28.21907545452534 20.93682839689555 18-19 19.946098841067105 29.633431534710695 30.477941002581694 19.942528621640506 20-21 21.54236556856226 28.981757455258684 29.350004454908397 20.12587252127066 22-23 20.478801026281175 29.06047244512543 29.706314967383673 20.75441156120972 24-25 20.70450460291312 28.685816510195185 29.53866212076045 21.071016766131248 26-27 20.626574219406084 28.791013225616847 29.537378762890654 21.045033792086414 28-29 20.22760884695291 28.71319045688061 30.082776561866392 20.976424134300082 30-31 21.196335258263595 29.131538596504612 29.16106097477248 20.511065170459315 32-33 20.044136857204393 29.126054839770514 30.02233688612459 20.8074714169005 34-35 20.2944740432324 28.522104925330517 30.03871061822282 21.144710413214266 36-37 21.028734703814226 29.067538810284205 29.221707614370846 20.682018871530726 38-39 20.681538999182845 28.991438394560546 29.57462883616806 20.75239377008855 40-41 20.942154887188224 28.74417483651407 29.29905836375257 21.014611912545135 42-43 20.563630708486176 28.8908407492927 29.58541051127675 20.96011803094438 44-45 21.036732514682328 28.356036915567078 29.36067424300206 21.246556326748532 46-47 21.009773800746395 29.084132001764157 29.18405613570033 20.72203806178912 48-49 20.861254720864444 28.79622800756864 29.266491253070072 21.076026018496847 50-51 21.16437246420016 28.719389232479354 28.820935648925232 21.295302654395254 52-53 20.589373757007863 29.026775776615004 28.948643207570058 21.43520725880708 54-55 21.02665900168495 28.883066920294564 28.619628944093844 21.470645133926634 56-57 21.171197215446668 28.705225720932308 28.68537833376227 21.438198729858755 58-59 20.572824380527443 28.296466295954637 29.420348908252507 21.710360415265413 60-61 21.33978389381654 28.768128853372904 28.542326692799225 21.349760560011326 62-63 20.56353619143193 29.077464868234333 28.949819783508325 21.40917915682542 64-65 20.598070507030062 28.314501526290968 29.140723127354867 21.946704839324102 66-67 20.91830935806617 28.64175716477585 28.80872028353218 21.631213193625804 68-69 21.11685362469512 28.548202581720457 28.85952805468953 21.47541573889489 70-71 21.250330318307842 28.507904143305357 28.501007890411827 21.740757647974974 72-73 20.864867734780567 28.771655792173966 28.972495461883945 21.39098101116152 74-75 21.266277208780043 28.14033604855357 28.707709770978624 21.885676971687765 76-77 21.038539144040392 28.71527643229234 28.738770514987245 21.507413908680025 78-79 21.03499965065337 28.60808708463982 28.980667554118977 21.376245710587828 80-81 21.19354191599182 28.458756757045368 28.603374880533682 21.74432644642913 82-83 20.528358921132707 28.799484643964394 28.922237130895915 21.749919304006976 84-85 20.85700495384053 28.210073104005023 28.70117056106203 22.231751381092423 86-87 21.014307488977106 28.598411580718814 28.52618158209782 21.861099348206256 88-89 20.67623989542943 28.206291044276373 29.00548891759729 22.111980142696908 90-91 21.0850723986173 28.24259207536296 28.598883912806507 22.07345161321323 92-93 20.40509198350467 28.719888282046647 28.804988563553312 22.07003117089537 94-95 20.422358402903235 28.040422843455058 29.036167807301894 22.50105094633981 96-97 20.768073288422922 28.341789944043754 28.798241004634672 22.091895762898652 98-99 20.604489721084764 28.119450729571287 28.822618891093132 22.453440658250816 100 20.729975113096607 28.037513899437855 28.679461020952456 22.55304996651308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14.0 1 11.0 2 16.0 3 22.0 4 24.5 5 30.5 6 68.5 7 146.0 8 217.0 9 236.5 10 214.0 11 219.0 12 248.0 13 304.0 14 400.0 15 506.0 16 639.5 17 783.0 18 952.0 19 1212.5 20 1583.0 21 2159.5 22 3057.5 23 4553.0 24 6750.0 25 10192.5 26 15579.0 27 23071.0 28 33326.0 29 45336.0 30 57997.0 31 71234.5 32 85012.0 33 98397.0 34 110382.0 35 121210.0 36 131241.5 37 139849.5 38 145575.5 39 148545.0 40 148538.5 41 145409.0 42 139701.0 43 133354.0 44 127073.0 45 119321.0 46 111909.5 47 106386.0 48 100419.0 49 96967.0 50 104510.0 51 99078.5 52 79413.5 53 69168.0 54 62941.0 55 57580.5 56 52398.5 57 46861.0 58 41442.5 59 36785.0 60 31520.0 61 25786.0 62 20819.5 63 15988.0 64 11635.0 65 8771.0 66 6543.5 67 4643.0 68 3483.5 69 2554.5 70 1785.0 71 1270.5 72 906.0 73 657.0 74 426.5 75 275.0 76 187.0 77 113.5 78 57.5 79 21.0 80 13.0 81 6.5 82 3.5 83 3.5 84 2.5 85 2.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7707652222775737 2 5.133309803019547 3 0.0 4 0.006497783279708826 5 0.1744639557589426 6 0.059059663988339374 7 0.036820771918350016 8 0.0436236154459325 9 0.04133566358688009 10-11 0.04301349495018519 12-13 0.041183133462943264 14-15 0.044310001003648215 16-17 0.03457857909647866 18-19 0.02841636208943085 20-21 0.023093060764035594 22-23 0.02353539812345239 24-25 0.02013397635966115 26-27 0.012949807522236604 28-29 0.020256000458810614 30-31 0.026418217465858422 32-33 0.0016778313633050957 34-35 0.003935277197570134 36-37 0.0042860964826248365 38-39 0.0063910121929530465 40-41 0.007199421849818231 42-43 0.00782479535795922 44-45 0.008877253213123324 46-47 0.016915590744594103 48-49 0.025442024672662728 50-51 0.022025349896477805 52-53 0.0280197837671951 54-55 0.02437431380510494 56-57 0.0162139521744847 58-59 0.014475108761604873 60-61 0.011988867741434595 62-63 0.018120578723695036 64-65 0.019874675148968544 66-67 0.020454289619928488 68-69 0.028187566903525613 70-71 0.02742491628384148 72-73 0.024404819829892302 74-75 0.025503036722237457 76-77 0.018913735368166536 78-79 0.015069976244958498 80-81 0.013529421993196546 82-83 0.009365349609721173 84-85 0.011653301468773575 86-87 0.009670409857594825 88-89 0.00776378330838449 90-91 0.007641759209235028 92-93 0.0043776145569869316 94-95 0.0013117590658567114 96-97 0.0010219518303767404 98-99 6.101204957473076E-4 100 0.1106758579285616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3278041.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.79687291456533 #Duplication Level Percentage of deduplicated Percentage of total 1 82.6364692432721 62.635859573406826 2 12.04389415906933 18.257790299429075 3 3.127389383577211 7.11139006984088 4 1.0698515397287776 3.243656047770968 5 0.4691743269843544 1.7780973418604917 6 0.2176284847930214 0.9897335162667633 7 0.11651774127216942 0.6182176299249179 8 0.07477199152169167 0.4533986511150897 9 0.046172623277334277 0.31497664128157865 >10 0.18559262475483132 2.3558979115244822 >50 0.007642385859039329 0.39352780038828067 >100 0.00376394546210545 0.5141299461047708 >500 6.466002462383889E-4 0.33232030699846044 >1k 4.0412515151854E-4 0.5680903663443159 >5k 8.082503030370799E-5 0.4329138977431368 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7874 0.240204439175715 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6259 0.1909372091441199 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.525301239368269E-5 0.0 22-23 0.0 0.0 0.0 1.2202409914946152E-4 0.0 24-25 0.0 0.0 0.0 2.1354217351155766E-4 0.0 26-27 0.0 0.0 0.0 3.2031326026733646E-4 0.0 28-29 0.0 0.0 0.0 8.84674718833596E-4 0.0 30-31 0.0 0.0 0.0 0.0033251567018228265 0.0 32-33 0.0 0.0 0.0 0.010051735167436893 0.0 34-35 0.0 0.0 0.0 0.0197373980374254 0.0 36-37 0.0 0.0 0.0 0.03679026589356265 0.0 38-39 0.0 0.0 0.0 0.06738780875529013 0.0 40-41 0.0 0.0 0.0 0.10622197830960625 0.0 42-43 0.0 0.0 0.0 0.14472058159126136 0.0 44-45 0.0 0.0 0.0 0.19240149833391346 0.0 46-47 0.0 0.0 0.0 0.25111034303719815 0.0 48-49 0.0 0.0 0.0 0.31427306735943816 0.0 50-51 0.0 0.0 0.0 0.3810049965817999 0.0 52-53 0.0 0.0 0.0 0.4513366367290708 0.0 54-55 0.0 0.0 0.0 0.5307133132257955 0.0 56-57 0.0 0.0 0.0 0.6240159900379525 0.0 58-59 0.0 0.0 0.0 0.7286364020462222 0.0 60-61 3.050602478736538E-5 0.0 0.0 0.8397088382970195 0.0 62-63 3.050602478736538E-5 0.0 0.0 0.957355322889494 0.0 64-65 3.050602478736538E-5 0.0 0.0 1.091475060867146 0.0 66-67 3.050602478736538E-5 0.0 0.0 1.2451186547087119 0.0 68-69 3.050602478736538E-5 0.0 0.0 1.4176607309060503 0.0 70-71 3.050602478736538E-5 3.050602478736538E-5 0.0 1.6157973618999883 0.0 72-73 3.050602478736538E-5 3.050602478736538E-5 0.0 1.821224932818107 0.0 74-75 3.050602478736538E-5 3.050602478736538E-5 0.0 2.035316214775837 0.0 76-77 3.050602478736538E-5 3.050602478736538E-5 0.0 2.2782967022072023 0.0 78-79 3.050602478736538E-5 3.050602478736538E-5 0.0 2.550334178248533 0.0 80-81 3.050602478736538E-5 3.050602478736538E-5 0.0 2.847600136789015 0.0 82-83 3.050602478736538E-5 3.050602478736538E-5 0.0 3.1665253729285263 0.0 84-85 3.050602478736538E-5 6.101204957473076E-5 0.0 3.497134416561599 0.0 86-87 3.050602478736538E-5 6.101204957473076E-5 0.0 3.855625966850323 0.0 88 3.050602478736538E-5 6.101204957473076E-5 0.0 4.1535478049237335 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13415 0.0 19.307533 1 GAGTACT 5080 0.0 17.32943 12-13 CAACGCA 14610 0.0 17.255892 5 TCAACGC 14845 0.0 17.046097 4 AACGCAG 14800 0.0 17.005766 6 ATCAACG 14975 0.0 16.992085 3 AGTACTT 5995 0.0 16.877373 12-13 TATCAAC 15180 0.0 16.78623 2 CATGGGG 4835 0.0 16.343145 4 GTACATG 16220 0.0 16.090954 1 TACATGG 16485 0.0 15.487458 2 ACGCAGA 16165 0.0 15.481209 7 CGCAGAG 16075 0.0 15.480261 8 TAGGACG 305 1.931916E-6 15.421372 4 GTATATA 2490 0.0 15.34399 1 ACATGGG 15930 0.0 15.264796 3 GTACTTT 6300 0.0 14.159779 14-15 TACCGAC 200 0.005703239 14.085576 7 CATGGGT 2405 0.0 14.08122 4 GCAGAGT 17375 0.0 13.997538 9 >>END_MODULE