FastQCFastQC Report
Wed 25 May 2016
SRR1295150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573741
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32970.12810146786331647No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28200.10956813447817787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG6400.019.0978831
GTGTAGG7950.018.4492781
TAAGACG2302.493226E-416.8492494
CTAGTAC5751.8189894E-1216.3678423
GTACATG135550.015.2388581
GTCCTAG8850.015.191991
TACACCG2305.669127E-415.1257655
GTGTATA17200.014.9229981
TAGAACG2606.3374813E-414.9051054
ACTGTCG2701.0944252E-414.7994588
GTATTAC9100.014.7746281
GTATTAG10400.014.69068051
GTATAAG8800.014.5838391
TACATGG137750.014.2820482
CATATAC14150.014.0849863
GGACCGC2851.7439344E-414.0103186
CATGGGT17150.013.8808054
CACGACT2800.00111052713.8404544
TAGTACT9200.013.8404544
GTGTATG16550.013.6627771
Produced by FastQC (version 0.11.5)