##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295150_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2573741 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.84998801355692 33.0 27.0 33.0 15.0 33.0 2 23.13604671177092 33.0 15.0 33.0 2.0 33.0 3 25.382534606240487 33.0 15.0 33.0 15.0 33.0 4 25.756458788976826 33.0 15.0 37.0 2.0 37.0 5 27.677266671355042 37.0 15.0 37.0 2.0 37.0 6 28.42024158608034 33.0 22.0 37.0 6.0 37.0 7 28.22302088671704 33.0 15.0 37.0 6.0 37.0 8 28.858897612463725 33.0 27.0 37.0 6.0 37.0 9 29.906958781011763 37.0 27.0 37.0 15.0 37.0 10-11 29.417290434429884 37.0 27.0 37.0 10.5 37.0 12-13 28.227607206785763 33.0 18.5 37.0 4.0 37.0 14-15 30.075734699023716 37.0 27.0 37.0 10.5 40.0 16-17 30.498494020960152 37.0 27.0 40.0 10.5 40.0 18-19 30.622436367917363 37.0 27.0 40.0 10.5 40.0 20-21 30.19811026051184 37.0 27.0 40.0 6.0 40.0 22-23 30.911552483330684 37.0 27.0 40.0 15.0 40.0 24-25 31.029975626918173 37.0 27.0 40.0 15.0 40.0 26-27 29.68439578807658 35.0 24.5 40.0 6.0 40.0 28-29 30.328467200079572 37.0 27.0 40.0 10.5 40.0 30-31 30.97649277841088 37.0 27.0 40.0 15.0 40.0 32-33 29.795884473223992 33.0 27.0 40.0 6.0 40.0 34-35 29.45236972173968 33.0 24.5 37.0 6.0 40.0 36-37 29.785178267743333 33.0 27.0 37.0 6.0 40.0 38-39 29.86791172849172 33.0 27.0 37.0 6.0 40.0 40-41 28.972037590417994 33.0 22.0 37.0 6.0 40.0 42-43 29.596473965329068 33.0 22.0 37.0 6.0 40.0 44-45 29.50405479805466 33.0 22.0 37.0 6.0 40.0 46-47 29.15380141203019 33.0 22.0 37.0 6.0 40.0 48-49 29.036768656986077 33.0 22.0 37.0 10.5 40.0 50-51 29.049250099368972 33.0 22.0 37.0 6.0 40.0 52-53 29.211256688221543 33.0 22.0 37.0 6.0 40.0 54-55 29.193368913189012 33.0 22.0 37.0 6.0 40.0 56-57 29.19697106274485 33.0 22.0 37.0 6.0 40.0 58-59 29.025836321525748 33.0 22.0 37.0 10.5 40.0 60-61 28.97667325500118 33.0 22.0 37.0 10.5 40.0 62-63 28.886490326726737 33.0 22.0 37.0 10.5 40.0 64-65 28.486662216594443 33.0 22.0 37.0 6.0 40.0 66-67 28.68597656096709 33.0 22.0 37.0 10.5 40.0 68-69 28.290754780686946 33.0 22.0 37.0 10.5 40.0 70-71 28.378515942357836 33.0 22.0 37.0 10.5 37.0 72-73 28.38078307024677 33.0 22.0 37.0 10.5 37.0 74-75 28.455108536562147 33.0 22.0 37.0 15.0 37.0 76-77 28.193070709135068 33.0 22.0 37.0 15.0 37.0 78-79 27.77435491760826 33.0 22.0 37.0 15.0 37.0 80-81 27.59959704570118 33.0 22.0 37.0 10.5 37.0 82-83 27.665423793614043 33.0 22.0 33.0 15.0 37.0 84-85 27.624083775329375 33.0 22.0 33.0 15.0 37.0 86-87 27.3726116963595 27.0 22.0 33.0 15.0 37.0 88-89 27.28246820484268 27.0 22.0 33.0 15.0 37.0 90-91 27.424542912437577 27.0 22.0 33.0 15.0 37.0 92-93 27.356496438452822 27.0 22.0 33.0 15.0 37.0 94-95 27.23995071765185 27.0 22.0 33.0 15.0 37.0 96-97 27.339579235051236 27.0 22.0 33.0 15.0 37.0 98-99 27.381734797712745 27.0 22.0 33.0 15.0 37.0 100 27.03298777926761 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.396920569420772 1101 2 11.330698990963409 1101 3 8.451884743861434 1101 4 13.000201482601742 1101 5 10.977439174058539 1101 6 10.094798093030658 1101 7 9.621203466747623 1101 8 9.101842802644722 1101 9 7.392669764830696 1101 10-11 7.981696005439726 1101 12-13 9.330652783321181 1101 14-15 8.115430409002283 1101 16-17 8.36583739637658 1101 18-19 8.393989870173968 1101 20-21 7.5155082192051665 1101 22-23 7.64951555129257 1101 24-25 7.508648701244173 1101 26-27 8.802496086368528 1101 28-29 8.457127865875975 1101 30-31 5.6989827451023665 1101 32-33 7.2135778855667 1101 34-35 8.883734258105573 1101 36-37 8.276612454202226 1101 38-39 8.314392703144986 1101 40-41 9.148990239519375 1101 42-43 8.698084807710956 1101 44-45 8.640354647094252 1101 46-47 7.37628922412814 1101 48-49 5.754850730505968 1101 50-51 7.122250459214797 1101 52-53 6.2301396367949025 1101 54-55 4.405407904641731 1101 56-57 6.442335974652206 1101 58-59 6.307642787907238 1101 60-61 6.9247568896327785 1101 62-63 6.511500113698585 1101 64-65 6.895936404208886 1101 66-67 5.986909500737557 1101 68-69 7.022619259542807 1101 70-71 6.4445319757370605 1101 72-73 5.6715785597321435 1101 74-75 5.086381551919143 1101 76-77 5.236424474465107 1101 78-79 5.3928726812824905 1101 80-81 5.111281953945458 1101 82-83 4.637370331392152 1101 84-85 4.520408924730543 1101 86-87 4.316761753206844 1101 88-89 4.313634423915204 1101 90-91 4.033197051185422 1101 92-93 4.022406641353161 1101 94-95 3.9499330875284073 1101 96-97 3.9345581438908646 1101 98-99 3.580394828219795 1101 100 3.892398176763443 1102 1 6.836938831545897 1102 2 10.507844964499046 1102 3 8.22861112792268 1102 4 12.762547188124596 1102 5 10.681775052718656 1102 6 9.623108915405343 1102 7 6.891467346684344 1102 8 8.123919090694546 1102 9 6.423352402179798 1102 10-11 7.524889650519352 1102 12-13 9.152884749940068 1102 14-15 6.239374912920081 1102 16-17 7.401699634369802 1102 18-19 7.523318791124517 1102 20-21 6.9809589622149275 1102 22-23 6.527258441676171 1102 24-25 7.043603559250503 1102 26-27 8.500448737599157 1102 28-29 7.725196101271692 1102 30-31 4.193496993550827 1102 32-33 4.4110468277126245 1102 34-35 5.143682409657021 1102 36-37 5.275509928210365 1102 38-39 4.610407207902433 1102 40-41 6.368933387012483 1102 42-43 7.043217630576578 1102 44-45 6.496892898662008 1102 46-47 5.681465117304935 1102 48-49 3.9866055358540002 1102 50-51 5.217218096735397 1102 52-53 5.850602408184287 1102 54-55 5.52682130980352 1102 56-57 6.259795928307984 1102 58-59 6.004314069479651 1102 60-61 6.109717570320267 1102 62-63 4.3740811572107 1102 64-65 6.26913722348613 1102 66-67 3.8712425822903285 1102 68-69 6.236268477273043 1102 70-71 5.307623840466913 1102 72-73 5.038390995015806 1102 74-75 4.302944739637535 1102 76-77 3.31966030053119 1102 78-79 4.423358171353872 1102 80-81 4.518171322243862 1102 82-83 4.059435336353847 1102 84-85 3.174044355006231 1102 86-87 3.3259643649232977 1102 88-89 3.5221090953106042 1102 90-91 3.3624990908066508 1102 92-93 3.188305768792393 1102 94-95 3.117107639238924 1102 96-97 3.3631569684046028 1102 98-99 3.1900614635088402 1102 100 3.262951093073603 1103 1 6.588883103538819 1103 2 10.840732540377715 1103 3 7.945711331772273 1103 4 12.008726019164921 1103 5 10.368946978562121 1103 6 9.100137034518202 1103 7 8.262660449574867 1103 8 8.26015463449961 1103 9 7.021284678682623 1103 10-11 7.388068172930911 1103 12-13 8.707643802455717 1103 14-15 8.413812200626307 1103 16-17 7.891114872067472 1103 18-19 7.217359134077071 1103 20-21 8.209418414104519 1103 22-23 6.802152592120496 1103 24-25 5.14550214645627 1103 26-27 5.927539444721763 1103 28-29 7.064714244955923 1103 30-31 6.044659881745158 1103 32-33 7.960348399043333 1103 34-35 7.21264352897445 1103 36-37 4.11942163124646 1103 38-39 6.313832065421796 1103 40-41 6.774856784640498 1103 42-43 7.577145921665242 1103 44-45 5.70828821285679 1103 46-47 6.407641489424396 1103 48-49 5.491551088745478 1103 50-51 6.418001784843142 1103 52-53 6.165208903541178 1103 54-55 5.1832736430131625 1103 56-57 5.959690777478318 1103 58-59 5.452265285917019 1103 60-61 4.999677725098483 1103 62-63 2.6522782895202646 1103 64-65 4.997636264893526 1103 66-67 4.138712248662042 1103 68-69 5.215114532771512 1103 70-71 3.77209482757943 1103 72-73 4.438837138999748 1103 74-75 3.2297788210536815 1103 76-77 3.4498410416208003 1103 78-79 2.8695067936446 1103 80-81 3.9757396546733084 1103 82-83 3.303720961865256 1103 84-85 3.174140675128587 1103 86-87 2.606034647838939 1103 88-89 2.7698612343041518 1103 90-91 2.6209163307259686 1103 92-93 2.1976660806798947 1103 94-95 2.7514501856462275 1103 96-97 2.8148723853329187 1103 98-99 2.604613844767048 1103 100 2.7351077539839572 1104 1 6.415004470679982 1104 2 9.700449819934853 1104 3 7.422439411052622 1104 4 12.263310341031268 1104 5 9.641266818419773 1104 6 8.110829872596494 1104 7 3.8679861712039703 1104 8 6.6199120732105605 1104 9 6.623025114625687 1104 10-11 7.277056224038073 1104 12-13 7.536226925725789 1104 14-15 7.216287549084196 1104 16-17 7.187651608425721 1104 18-19 6.901483911657152 1104 20-21 7.694848982016737 1104 22-23 6.519703995837379 1104 24-25 5.934353038607316 1104 26-27 8.436908266390404 1104 28-29 7.338878625639985 1104 30-31 6.376608651021545 1104 32-33 7.680088167726609 1104 34-35 8.01921016845645 1104 36-37 6.5762069836584836 1104 38-39 7.32641300156256 1104 40-41 7.314022153857575 1104 42-43 7.474769735425625 1104 44-45 6.890382481836621 1104 46-47 6.748567065244881 1104 48-49 5.677501563241325 1104 50-51 5.774477602854212 1104 52-53 6.060226079302346 1104 54-55 4.957162161202174 1104 56-57 6.0643341024091235 1104 58-59 4.057023836727616 1104 60-61 4.419058841646528 1104 62-63 3.8396201242375447 1104 64-65 2.956998908230812 1104 66-67 3.3597723267561506 1104 68-69 4.238849828342886 1104 70-71 2.6387846629886837 1104 72-73 2.6284902874774048 1104 74-75 3.262329359804177 1104 76-77 3.874486405400951 1104 78-79 4.330047026938388 1104 80-81 3.093458491169951 1104 82-83 3.0498598136841544 1104 84-85 3.0827925014433397 1104 86-87 0.7480021992426558 1104 88-89 0.28598575239430346 1104 90-91 2.1611236755262837 1104 92-93 2.49207839780129 1104 94-95 2.505391034301823 1104 96-97 2.965197365562261 1104 98-99 2.5957485571039385 1104 100 0.886033885854264 1105 1 5.912487387762649 1105 2 -2.8880273295256913 1105 3 0.3508085062368984 1105 4 8.604084593000614 1105 5 8.77732788580952 1105 6 8.205928837110338 1105 7 5.346652715293242 1105 8 6.115263932046581 1105 9 5.1489184183229 1105 10-11 5.198072761946175 1105 12-13 5.370476896388791 1105 14-15 6.012975386498876 1105 16-17 6.220148230749071 1105 18-19 6.282733131167532 1105 20-21 7.152509405184865 1105 22-23 2.1667725396116317 1105 24-25 4.223393976111044 1105 26-27 7.03305887594103 1105 28-29 6.137403037472431 1105 30-31 5.382188077095773 1105 32-33 7.18987637030061 1105 34-35 6.277556496876439 1105 36-37 6.328971972039778 1105 38-39 4.35251097395253 1105 40-41 5.7324382572643735 1105 42-43 5.697383570815891 1105 44-45 6.401024328253836 1105 46-47 5.635442273506179 1105 48-49 5.097488827533464 1105 50-51 6.086013945274381 1105 52-53 5.768860553504929 1105 54-55 5.376992386278118 1105 56-57 5.184294649639803 1105 58-59 1.850273487978189 1105 60-61 4.418444945385911 1105 62-63 -0.9434296495067542 1105 64-65 -0.24852212623874692 1105 66-67 0.9765140093254558 1105 68-69 3.014477734195914 1105 70-71 3.1894304220513057 1105 72-73 2.807998803364253 1105 74-75 2.7238956667501135 1105 76-77 1.8185560806105912 1105 78-79 3.33260351869799 1105 80-81 1.69084357642922 1105 82-83 1.0333059820214103 1105 84-85 1.4220309067406909 1105 86-87 -0.2249477565479019 1105 88-89 1.6458829254374017 1105 90-91 1.4464074380164682 1105 92-93 1.7664655526523099 1105 94-95 1.1631325970130248 1105 96-97 2.137765234428713 1105 98-99 2.0390947380545335 1105 100 2.114004437157611 1106 1 6.262805925508516 1106 2 10.432182193155494 1106 3 7.075296243388436 1106 4 12.025301934568962 1106 5 10.103357489925756 1106 6 9.512246409518202 1106 7 6.675839111191031 1106 8 7.6061102847521305 1106 9 6.993621326409897 1106 10-11 5.928176285304648 1106 12-13 8.202301315081979 1106 14-15 8.060586585096 1106 16-17 7.941475904264447 1106 18-19 7.422046634077077 1106 20-21 8.321379825089366 1106 22-23 6.362833621160899 1106 24-25 5.201744255042133 1106 26-27 8.015556332348027 1106 28-29 6.484916265157942 1106 30-31 5.54720486280576 1106 32-33 7.5317084842706095 1106 34-35 6.97941475687848 1106 36-37 6.227096031498991 1106 38-39 7.307163820472301 1106 40-41 6.805147977822315 1106 42-43 6.321718601589481 1106 44-45 4.8239072942961805 1106 46-47 6.349250943338543 1106 48-49 6.059567187230325 1106 50-51 6.802321544944149 1106 52-53 5.795448802531077 1106 54-55 5.545114709932353 1106 56-57 4.985835032528822 1106 58-59 5.1909233509422705 1106 60-61 5.0161727535075755 1106 62-63 5.160578084343495 1106 64-65 5.3271679094642295 1106 66-67 3.109016857247898 1106 68-69 3.41279642986747 1106 70-71 3.0221125832612508 1106 72-73 2.961382909201763 1106 74-75 3.016978947316307 1106 76-77 3.6440309879591837 1106 78-79 4.2210949407405565 1106 80-81 3.7591459835874517 1106 82-83 2.1407218299208104 1106 84-85 2.7911427268962647 1106 86-87 3.4495459325611613 1106 88-89 3.437184861198091 1106 90-91 3.37445168426132 1106 92-93 3.030902744189998 1106 94-95 1.5807608706209706 1106 96-97 1.5801343802824164 1106 98-99 2.137645331509475 1106 100 2.9935318385799192 1107 1 6.212127564812281 1107 2 10.55794367524744 1107 3 6.344880646534293 1107 4 11.635425254929547 1107 5 9.614539013487281 1107 6 8.232916123969378 1107 7 3.92532428500688 1107 8 6.626176230088426 1107 9 5.873595574976282 1107 10-11 4.6457770383581725 1107 12-13 6.254737146579426 1107 14-15 6.2269382771884345 1107 16-17 7.2190084112097885 1107 18-19 6.506252353963035 1107 20-21 5.677995607321435 1107 22-23 5.867024493601452 1107 24-25 4.6742321867469165 1107 26-27 7.448734601924578 1107 28-29 6.550658860165392 1107 30-31 5.126751456347197 1107 32-33 5.333114347011975 1107 34-35 6.906668918516697 1107 36-37 6.596513812188096 1107 38-39 6.545753864938707 1107 40-41 7.726855292302655 1107 42-43 6.3562717532166815 1107 44-45 4.247045377563371 1107 46-47 3.7054984502028887 1107 48-49 4.255513728327326 1107 50-51 4.9892474909689 1107 52-53 4.213347441000593 1107 54-55 5.532079154021321 1107 56-57 5.417251275320243 1107 58-59 3.183761534036126 1107 60-61 3.140733164335831 1107 62-63 0.6742738149815786 1107 64-65 2.270370039741074 1107 66-67 4.342221222487968 1107 68-69 5.228791945260625 1107 70-71 4.301216018776866 1107 72-73 2.451060821386694 1107 74-75 2.6392969914425315 1107 76-77 3.200980217592921 1107 78-79 4.033880259234209 1107 80-81 2.3163259231852287 1107 82-83 1.823442033279683 1107 84-85 2.454237206866136 1107 86-87 2.3176334292201055 1107 88-89 2.6985774154041735 1107 90-91 1.6519809640513792 1107 92-93 2.33474783010573 1107 94-95 2.3861842253033103 1107 96-97 2.463139244048371 1107 98-99 1.8591172155201932 1107 100 0.28536526269544993 1108 1 4.326864628696054 1108 2 -3.4839380196702017 1108 3 -0.1251636495095454 1108 4 6.822092670857042 1108 5 7.153198438580091 1108 6 6.823636304509218 1108 7 4.329418835319103 1108 8 5.159613211169269 1108 9 4.922981949572112 1108 10-11 3.5233357960965286 1108 12-13 2.6721403302335 1108 14-15 4.226821101120468 1108 16-17 4.694605578491551 1108 18-19 4.064047420240598 1108 20-21 4.718041645236596 1108 22-23 4.198285416319358 1108 24-25 2.9369945000801074 1108 26-27 4.72485338602155 1108 28-29 6.162881033290752 1108 30-31 4.493277076264473 1108 32-33 4.635323129501558 1108 34-35 3.0730515384832806 1108 36-37 1.398273598527087 1108 38-39 2.698734450117815 1108 40-41 5.413900332484165 1108 42-43 3.31538996928429 1108 44-45 3.9113072211676574 1108 46-47 4.7092463393046025 1108 48-49 4.967107018664617 1108 50-51 2.1467456546394885 1108 52-53 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46-47 -17.533972247025467 2213 48-49 -6.150166390929318 2213 50-51 -24.074098909601304 2213 52-53 -18.42427703819324 2213 54-55 -18.307220090049167 2213 56-57 -18.20879033702033 2213 58-59 -18.645710662592467 2213 60-61 -19.99020413465761 2213 62-63 -18.16054352619789 2213 64-65 -18.26969943971352 2213 66-67 -8.889611768953134 2213 68-69 -23.45554518945071 2213 70-71 -18.061130528111626 2213 72-73 -9.858753477531419 2213 74-75 -4.48508821824171 2213 76-77 -3.4251504895445137 2213 78-79 -4.721719493618274 2213 80-81 -3.3941324659228975 2213 82-83 -3.297691216319027 2213 84-85 -3.125658190047563 2213 86-87 -3.322136930751846 2213 88-89 -2.7491999953706454 2213 90-91 -2.86276422482959 2213 92-93 -3.835396249943411 2213 94-95 -3.861123916657295 2213 96-97 -3.5280222623635566 2213 98-99 -3.8136153218996185 2213 100 -3.599093634904932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 701.0 3 562.0 4 2534.0 5 9850.0 6 16653.0 7 19398.0 8 17578.0 9 17495.0 10 21058.0 11 26777.0 12 33515.0 13 45134.0 14 34399.0 15 37496.0 16 43559.0 17 46729.0 18 47127.0 19 48235.0 20 48105.0 21 46591.0 22 48916.0 23 54422.0 24 60660.0 25 67203.0 26 74208.0 27 80322.0 28 87826.0 29 93120.0 30 98747.0 31 109076.0 32 124791.0 33 152610.0 34 186641.0 35 222101.0 36 248397.0 37 224664.0 38 76319.0 39 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.186838890484275 20.9694904431786 14.12498160907372 23.718689057263408 2 14.831022004574827 21.22409541978775 40.937985093301556 23.00689748233587 3 17.948488175595827 27.06929642215739 29.457032304952108 25.52518309729468 4 11.903474002329958 17.754665176134253 39.50081187938307 30.841048942152717 5 12.8728596070512 37.82916649136831 36.21444949303715 13.08352440854334 6 29.98889031393357 38.25910879593256 18.73014079913297 13.0218600910009 7 25.63714279612059 31.992761801542535 24.68006233125772 17.69003307107916 8 23.48055128460264 36.73215273350141 22.419397294955175 17.367898686940773 9 24.060981663994532 17.543846084982054 22.493306096538703 35.901866154484715 10-11 22.798030593284704 27.87931284101479 29.3539859649428 19.968670600757697 12-13 23.77012946275929 25.407082558238557 29.461475489459783 21.36131248954237 14-15 21.28016762465951 26.873638314778265 26.733874850041765 25.11231921052046 16-17 20.68678793677184 30.584607935049046 27.703299984193798 21.02530414398532 18-19 20.763395401803066 29.594295490511733 29.21520358080002 20.427105526885185 20-21 22.100476723221725 28.987098378907145 28.228731079192194 20.68369381867894 22-23 21.00079820398979 29.038128329478933 28.65599457344245 21.305078893088826 24-25 21.320711696509676 28.81929531097871 28.159763393255925 21.70022959925569 26-27 21.379913690190406 28.624866158902623 28.30034740667105 21.69487274423592 28-29 21.1375046769177 28.604893992612528 28.794031016823716 21.463570313646052 30-31 21.84716912520484 29.068251191941428 28.04116121888405 21.043418463969683 32-33 20.751742354810098 28.843745105596685 28.847818937789416 21.5566936018038 34-35 20.848176016269854 28.394481766382064 28.687123534173846 22.07021868317424 36-37 21.608349987316995 29.001685072359294 28.10328991203761 21.286675028286105 38-39 21.402130709952722 28.565737256226033 28.401823859805404 21.63030817401585 40-41 21.446466938784088 28.73408664680945 28.0721373825246 21.747309031881866 42-43 21.250781991332424 28.76596097404469 28.57083944527174 21.412417589351143 44-45 21.763475383714812 28.24783245774801 28.37563784258743 21.613054315949743 46-47 21.458928557133376 28.794069995547815 28.63652962056683 21.11047182675198 48-49 21.353917626958744 28.507098695840444 28.711418302482887 21.42756537471793 50-51 21.53835610104106 28.583911608888652 28.41385565845796 21.463876631612326 52-53 20.953376728916286 28.668260949803148 28.47816919420845 21.90019312707211 54-55 21.405159056136647 28.715582390599394 28.124205247459027 21.755053305804932 56-57 21.63610893374776 28.543352713784042 28.087899267661708 21.732639084806486 58-59 21.305097511936108 28.05064279384754 28.674671795925228 21.969587898291124 60-61 21.83056684416899 28.586696080492107 28.054209282219013 21.528527793119892 62-63 21.0929305391205 28.735211450159976 28.601457432738954 21.57040057798057 64-65 21.042618399689086 28.275293187945895 28.618347438069886 22.063740974295133 66-67 21.396418450823766 28.391063745012797 28.303152739710924 21.909365064452512 68-69 21.845810766853123 28.299530826067947 28.222959231728957 21.63169917534997 70-71 21.843716108962894 28.469414484020163 27.92908423674679 21.757785170270157 72-73 21.515783934459613 28.54527762082675 28.447535805349823 21.49140263936382 74-75 21.819114784050424 28.068884833938835 28.094130420658015 22.017869961352726 76-77 21.642084873316065 28.54595322877455 28.318807207482482 21.4931546904269 78-79 21.700195462414822 28.28386130811041 28.469517058268952 21.54642617120582 80-81 21.69024385077091 28.262745646950236 28.290898493545534 21.75611200873332 82-83 21.13916634968043 28.547878227193817 28.528682949584315 21.78427247354143 84-85 21.410928565565385 28.168063741652606 28.104634717552322 22.31637297522969 86-87 21.702979044562547 28.35520557198678 28.115989140432536 21.82582624301814 88-89 21.43840817106714 27.956217817648454 28.552523691121422 22.05285032016298 90-91 21.840029236204167 28.046544037032522 28.157271287611156 21.956155439152152 92-93 21.190294622114013 28.384845154667985 28.58581112433951 21.839049098878494 94-95 21.13259617274929 27.9461613383293 28.51157505065628 22.409667438265128 96-97 21.49837813825222 28.124072277318746 28.41729363885504 21.96025594557399 98-99 21.503317982044045 27.889626044778463 28.402051183583037 22.20500478959445 100 21.546654663125935 27.813056815025856 28.247665082490535 22.392623439357674 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 298.0 1 349.0 2 340.0 3 278.0 4 309.5 5 408.0 6 579.0 7 832.0 8 1211.5 9 1709.0 10 2364.5 11 3132.0 12 3973.0 13 4819.0 14 5573.0 15 6398.0 16 7196.0 17 7829.0 18 8544.5 19 9313.0 20 10085.0 21 11018.5 22 12287.0 23 13841.5 24 15814.0 25 18575.0 26 22590.0 27 27801.5 28 33969.0 29 41554.0 30 49713.0 31 57656.5 32 65646.5 33 73140.5 34 80049.5 35 86276.0 36 92255.5 37 96928.0 38 99501.0 39 101419.5 40 101956.0 41 100653.0 42 97895.5 43 93858.0 44 89438.5 45 85280.5 46 81487.0 47 78180.0 48 75607.0 49 74753.5 50 75626.5 51 71517.0 52 61876.5 53 54092.0 54 48712.5 55 44261.5 56 40340.5 57 36511.5 58 32533.5 59 28449.5 60 24509.5 61 20724.5 62 17100.0 63 13822.5 64 11131.5 65 8892.0 66 6952.0 67 5432.0 68 4147.0 69 3171.5 70 2416.0 71 1782.5 72 1385.0 73 1064.0 74 794.0 75 591.5 76 434.5 77 298.5 78 194.0 79 127.5 80 87.0 81 54.0 82 40.5 83 35.5 84 28.5 85 19.0 86 10.5 87 8.5 88 6.5 89 6.5 90 4.5 91 0.5 92 1.5 93 1.5 94 0.0 95 1.5 96 1.5 97 0.0 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 6.778459837256352 2 22.832678191006785 3 5.479106094980031 4 22.424128923617413 5 11.342128054066047 6 8.510530002824682 7 7.221161725286267 8 8.04191253121429 9 4.51797597349539 10-11 6.205538941175511 12-13 9.205665993586768 14-15 6.578808823420849 16-17 7.082103443975132 18-19 6.137680520300994 20-21 6.977566895814304 22-23 5.202096869887064 24-25 4.74321231235 26-27 8.755368158645334 28-29 5.708771783951843 30-31 3.891242358885374 32-33 7.963660679143706 34-35 6.291173043441434 36-37 6.0282095206938076 38-39 5.3501304132777925 40-41 7.54751546484281 42-43 5.78309938723438 44-45 5.681302042435505 46-47 5.35630819107284 48-49 3.587559898218197 50-51 5.890142015066784 52-53 3.995312659665444 54-55 4.362637887806116 56-57 4.284114058096755 58-59 3.2525611551434275 60-61 3.6945442451280064 62-63 2.875308743187446 64-65 4.075371220336468 66-67 2.192236903402479 68-69 4.503580585614482 70-71 2.176656470095476 72-73 1.3563719115482094 74-75 0.7319695338419833 76-77 1.071650177698533 78-79 1.5444444487615498 80-81 1.8743727515705737 82-83 0.7158839992058252 84-85 1.0719027283631104 86-87 0.43547505362816225 88-89 0.7028484995187939 90-91 0.3274805040600433 92-93 0.3159797353346743 94-95 0.4898511544090878 96-97 0.4671993024939184 98-99 0.4435372479204396 100 0.3905987432301852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2573741.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.76388310961434 #Duplication Level Percentage of deduplicated Percentage of total 1 97.62305180873996 93.48762522215996 2 1.9344692714526817 3.705045783810708 3 0.24826557406472552 0.7132462624462702 4 0.07864713433204026 0.3012621991631856 5 0.03532203372142297 0.16912875542461023 6 0.02089483096273352 0.12005820899462177 7 0.01527099252785944 0.10236866803840078 8 0.007994707942345993 0.06124834215090615 9 0.007645288940650059 0.06589283008064754 >10 0.025654307823344498 0.4001097444210694 >50 0.0011900162360341721 0.07909174074084487 >100 0.0011855559286966608 0.2835407986222906 >500 2.4508639650239243E-4 0.18248255457986676 >1k 1.633909309998561E-4 0.3288988893666157 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3297 0.12810146786331647 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2820 0.10956813447817787 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.8853948396516975E-5 0.0 4 0.0 0.0 0.0 7.770789679303395E-5 0.0 5 0.0 0.0 0.0 7.770789679303395E-5 0.0 6 0.0 0.0 0.0 7.770789679303395E-5 0.0 7 0.0 3.8853948396516975E-5 0.0 1.1656184518955093E-4 0.0 8 0.0 3.8853948396516975E-5 0.0 1.1656184518955093E-4 0.0 9 0.0 3.8853948396516975E-5 0.0 1.1656184518955093E-4 0.0 10-11 0.0 3.8853948396516975E-5 0.0 1.1656184518955093E-4 0.0 12-13 0.0 3.8853948396516975E-5 0.0 1.1656184518955093E-4 0.0 14-15 0.0 3.8853948396516975E-5 0.0 1.554157935860679E-4 0.0 16-17 0.0 3.8853948396516975E-5 0.0 1.7484276778432638E-4 0.0 18-19 0.0 3.8853948396516975E-5 0.0 2.3312369037910188E-4 0.0 20-21 0.0 3.8853948396516975E-5 0.0 2.7197763877561884E-4 0.0 22-23 0.0 3.8853948396516975E-5 0.0 3.302585613703943E-4 0.0 24-25 1.9426974198258488E-5 3.8853948396516975E-5 0.0 4.856743549564622E-4 0.0 26-27 3.8853948396516975E-5 3.8853948396516975E-5 0.0 5.439552775512377E-4 0.0 28-29 3.8853948396516975E-5 3.8853948396516975E-5 0.0 7.187980453355641E-4 0.0 30-31 3.8853948396516975E-5 3.8853948396516975E-5 0.0 0.0019621243940241076 0.0 32-33 5.828092259477546E-5 3.8853948396516975E-5 0.0 0.005692103440089737 0.0 34-35 7.770789679303395E-5 3.8853948396516975E-5 0.0 0.010995667396214305 0.0 36-37 7.770789679303395E-5 7.770789679303395E-5 0.0 0.01977665973382714 0.0 38-39 7.770789679303395E-5 1.1656184518955093E-4 0.0 0.032967575214444655 0.0 40-41 7.770789679303395E-5 1.1656184518955093E-4 0.0 0.051500908599583256 0.0 42-43 7.770789679303395E-5 1.554157935860679E-4 0.0 0.06916002814580022 0.0 44-45 7.770789679303395E-5 1.554157935860679E-4 0.0 0.09068511555747062 0.0 46-47 7.770789679303395E-5 1.554157935860679E-4 0.0 0.11790230640923077 0.0 48-49 7.770789679303395E-5 1.554157935860679E-4 0.0 0.1469456328356272 0.0 50-51 7.770789679303395E-5 1.554157935860679E-4 0.0 0.17861160077878854 0.0 52-53 1.1656184518955093E-4 1.942697419825849E-4 0.0 0.21319161485168864 0.0 54-55 1.1656184518955093E-4 1.942697419825849E-4 0.0 0.24985031516380243 0.0 56-57 1.1656184518955093E-4 1.942697419825849E-4 0.0 0.29400782751644394 0.0 58-59 1.1656184518955093E-4 1.942697419825849E-4 0.0 0.34550873611602717 0.0 60-61 1.1656184518955093E-4 1.942697419825849E-4 0.0 0.402157792878149 0.0 62-63 1.1656184518955093E-4 1.942697419825849E-4 0.0 0.46127407536344955 0.0 64-65 1.1656184518955093E-4 1.942697419825849E-4 0.0 0.5282582823990448 0.0 66-67 1.1656184518955093E-4 2.1369671618084337E-4 0.0 0.6062770107792509 0.0 68-69 1.1656184518955093E-4 2.3312369037910185E-4 0.0 0.6975255085884711 0.0 70-71 1.1656184518955093E-4 2.3312369037910185E-4 0.0 0.8078707220345793 0.0 72-73 1.1656184518955093E-4 2.3312369037910185E-4 0.0 0.9210911276620297 0.0 74-75 1.1656184518955093E-4 2.3312369037910185E-4 0.0 1.0380997932581406 0.0 76-77 1.3598881938780942E-4 2.3312369037910185E-4 0.0 1.1695427006835575 0.0 78-79 1.554157935860679E-4 2.3312369037910185E-4 0.0 1.3207816948169997 3.8853948396516975E-5 80-81 1.554157935860679E-4 2.3312369037910185E-4 0.0 1.4919721914520538 3.8853948396516975E-5 82-83 1.554157935860679E-4 2.3312369037910185E-4 0.0 1.6810743582979017 3.8853948396516975E-5 84-85 1.942697419825849E-4 2.3312369037910185E-4 0.0 1.8695160080210091 3.8853948396516975E-5 86-87 1.942697419825849E-4 2.7197763877561884E-4 0.0 2.0738100686898955 3.8853948396516975E-5 88 1.942697419825849E-4 2.7197763877561884E-4 0.0 2.2500710055906947 3.8853948396516975E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 640 0.0 19.097883 1 GTGTAGG 795 0.0 18.449278 1 TAAGACG 230 2.493226E-4 16.849249 4 CTAGTAC 575 1.8189894E-12 16.367842 3 GTACATG 13555 0.0 15.238858 1 GTCCTAG 885 0.0 15.19199 1 TACACCG 230 5.669127E-4 15.125765 5 GTGTATA 1720 0.0 14.922998 1 TAGAACG 260 6.3374813E-4 14.905105 4 ACTGTCG 270 1.0944252E-4 14.799458 8 GTATTAC 910 0.0 14.774628 1 GTATTAG 1040 0.0 14.6906805 1 GTATAAG 880 0.0 14.583839 1 TACATGG 13775 0.0 14.282048 2 CATATAC 1415 0.0 14.084986 3 GGACCGC 285 1.7439344E-4 14.010318 6 CATGGGT 1715 0.0 13.880805 4 CACGACT 280 0.001110527 13.840454 4 TAGTACT 920 0.0 13.840454 4 GTGTATG 1655 0.0 13.662777 1 >>END_MODULE