FastQCFastQC Report
Wed 25 May 2016
SRR1295150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573741
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT85770.3332503153969261No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69410.26968525582022435No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG28840.11205478717555496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1659.249743E-825.658425
TACACCG2151.1853572E-619.6913475
GAGTACT37750.017.53865612-13
GTCTCGC4303.6379788E-1117.2512071
GTATAGA11900.015.7918621
TACGACC1800.00279526515.6801484
CGTAGAC2104.3135553E-415.6798423
GTATAGG7350.015.4752331
GACCGCG1850.003394846815.2293757
AGTACTT44550.015.07240112-13
TCCGACG1106.23329E-414.93847330-31
GGACCGA1900.0040512614.8300436
GTGTATA18600.014.3574571
GTCTTAG8650.014.2929091
CTAGTAC6300.014.18652253
CTACACT11750.014.0120474
GTACAGG12450.013.902581
GTACTTT48700.013.83553514-15
CATGGGG41050.013.7511644
GAGTGCG2802.5180975E-413.4163547
Produced by FastQC (version 0.11.5)