##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295150_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2573741 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.548808524245448 33.0 33.0 33.0 27.0 33.0 2 30.345177700475688 33.0 33.0 33.0 27.0 33.0 3 31.08006710853967 33.0 33.0 33.0 27.0 33.0 4 35.30327099735366 37.0 37.0 37.0 33.0 37.0 5 35.60223386890911 37.0 37.0 37.0 33.0 37.0 6 35.42322751201461 37.0 37.0 37.0 33.0 37.0 7 35.33170470532971 37.0 37.0 37.0 33.0 37.0 8 35.341131838829156 37.0 37.0 37.0 33.0 37.0 9 35.28737390436722 37.0 37.0 37.0 33.0 37.0 10-11 35.26869312024792 37.0 37.0 37.0 33.0 37.0 12-13 35.196918804184264 37.0 37.0 37.0 33.0 37.0 14-15 36.722956971971925 40.0 37.0 40.0 33.0 40.0 16-17 36.728512115243916 40.0 37.0 40.0 33.0 40.0 18-19 36.75380079036702 40.0 37.0 40.0 33.0 40.0 20-21 36.751748136273235 40.0 37.0 40.0 33.0 40.0 22-23 36.71176450932708 40.0 37.0 40.0 33.0 40.0 24-25 36.68919425070355 40.0 37.0 40.0 33.0 40.0 26-27 36.66932511857254 40.0 37.0 40.0 33.0 40.0 28-29 36.61156619877447 40.0 37.0 40.0 33.0 40.0 30-31 36.519862138420294 40.0 37.0 40.0 33.0 40.0 32-33 36.47823382383853 40.0 37.0 40.0 33.0 40.0 34-35 36.400558370092405 40.0 37.0 40.0 33.0 40.0 36-37 36.38380357619512 40.0 37.0 40.0 33.0 40.0 38-39 36.320686502643426 37.0 37.0 40.0 33.0 40.0 40-41 36.25578020476808 37.0 37.0 40.0 33.0 40.0 42-43 36.16056258186041 37.0 37.0 40.0 27.0 40.0 44-45 36.057331137826225 37.0 37.0 40.0 27.0 40.0 46-47 35.949230711248724 37.0 37.0 40.0 27.0 40.0 48-49 35.835410400658034 37.0 37.0 40.0 27.0 40.0 50-51 35.73905241436493 37.0 37.0 40.0 27.0 40.0 52-53 35.64803354339073 37.0 37.0 40.0 27.0 40.0 54-55 35.53418972616126 37.0 37.0 40.0 27.0 40.0 56-57 35.411571521765396 37.0 35.0 40.0 27.0 40.0 58-59 35.284555244680796 37.0 33.0 40.0 27.0 40.0 60-61 35.12597945947164 37.0 33.0 40.0 27.0 40.0 62-63 34.95057836044886 37.0 33.0 40.0 27.0 40.0 64-65 34.77323320411806 37.0 33.0 40.0 27.0 40.0 66-67 34.552005038580035 37.0 33.0 40.0 27.0 40.0 68-69 34.33459038807712 37.0 33.0 38.5 27.0 40.0 70-71 34.098806562121055 37.0 33.0 37.0 27.0 40.0 72-73 33.87702550489735 37.0 33.0 37.0 27.0 40.0 74-75 33.63067670756304 37.0 33.0 37.0 27.0 40.0 76-77 33.38697580681195 37.0 33.0 37.0 27.0 38.5 78-79 33.1531082575908 37.0 33.0 37.0 24.5 37.0 80-81 32.950059465968025 37.0 33.0 37.0 22.0 37.0 82-83 32.73724259744861 37.0 33.0 37.0 22.0 37.0 84-85 32.55607732091147 37.0 33.0 37.0 22.0 37.0 86-87 32.57421997784547 37.0 33.0 37.0 22.0 37.0 88-89 32.589947084807676 37.0 33.0 37.0 22.0 37.0 90-91 32.59806095485132 37.0 33.0 37.0 22.0 37.0 92-93 32.561869084729196 37.0 33.0 37.0 22.0 37.0 94-95 32.58797310995939 37.0 33.0 37.0 22.0 37.0 96-97 32.550425237038226 37.0 33.0 37.0 22.0 37.0 98-99 32.474634199789335 33.0 33.0 37.0 22.0 37.0 100 31.939061855874385 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5434361818680173 1101 2 4.079478935900784 1101 3 2.3478356596823 1101 4 1.1283501354208525 1101 5 0.44940935138299665 1101 6 0.5059905057666114 1101 7 0.5287553120536543 1101 8 0.5143970826932289 1101 9 0.6341357629261566 1101 10-11 0.6154456321428228 1101 12-13 0.6195979739842628 1101 14-15 0.9473837951368651 1101 16-17 0.8862715987455232 1101 18-19 0.8928755254679572 1101 20-21 0.9258491575738859 1101 22-23 0.8699213292234376 1101 24-25 0.9053706032340045 1101 26-27 0.8848154397682748 1101 28-29 0.8691009157679161 1101 30-31 0.7465928449375099 1101 32-33 0.9988724973650065 1101 34-35 1.051744046175763 1101 36-37 0.9098476365534296 1101 38-39 0.9039436198972908 1101 40-41 0.9359306152124489 1101 42-43 1.062532952831198 1101 44-45 1.0971075095557765 1101 46-47 1.0728734575603411 1101 48-49 0.9186538813730039 1101 50-51 0.9008825630153083 1101 52-53 0.9359289513199585 1101 54-55 0.9492347870206075 1101 56-57 0.9859074812782538 1101 58-59 0.9849423910889854 1101 60-61 0.9755809178203236 1101 62-63 1.0328813607301868 1101 64-65 0.9846068483703192 1101 66-67 0.9500864702013843 1101 68-69 0.9713090294364193 1101 70-71 0.9809304198800533 1101 72-73 0.8941235897655986 1101 74-75 0.8567955194708716 1101 76-77 0.8943011427541734 1101 78-79 1.027419154040885 1101 80-81 1.0413937529912758 1101 82-83 0.977371939079994 1101 84-85 1.0370816799682459 1101 86-87 0.9765739265436295 1101 88-89 0.893203483996615 1101 90-91 0.9077408360414765 1101 92-93 0.9361216694065959 1101 94-95 0.8525267039170075 1101 96-97 0.8011784379945794 1101 98-99 0.8915220277723819 1101 100 1.0149484600245593 1102 1 0.43289537019035507 1102 2 3.9041193831481387 1102 3 2.2176503546804405 1102 4 0.971679899184771 1102 5 0.35318316163511554 1102 6 0.3356136547134838 1102 7 0.38086277496627474 1102 8 0.39490450496101914 1102 9 0.3885465826772787 1102 10-11 0.45908771131855275 1102 12-13 0.4699070084773851 1102 14-15 0.7337924390224515 1102 16-17 0.6853659892276696 1102 18-19 0.6996243021644233 1102 20-21 0.7324066447325848 1102 22-23 0.7354429435826759 1102 24-25 0.706340814420571 1102 26-27 0.6385440738444075 1102 28-29 0.6850697897536975 1102 30-31 0.6742373837212625 1102 32-33 0.7656463183100541 1102 34-35 0.8320890235274945 1102 36-37 0.6996979144732407 1102 38-39 0.6381214990962576 1102 40-41 0.5922643600775785 1102 42-43 0.6950437805932452 1102 44-45 0.6910193223179775 1102 46-47 0.7671809438571628 1102 48-49 0.6039861706554959 1102 50-51 0.6093625808765992 1102 52-53 0.5993974526599928 1102 54-55 0.6622992679117843 1102 56-57 0.6110732874560725 1102 58-59 0.6959304127265682 1102 60-61 0.6213107218083707 1102 62-63 0.7466542337439108 1102 64-65 0.7062279096829442 1102 66-67 0.6553410937403612 1102 68-69 0.5683739715844496 1102 70-71 0.5455530800489328 1102 72-73 0.6667042715878253 1102 74-75 0.663051165032094 1102 76-77 0.5498328885201929 1102 78-79 0.4744648196960455 1102 80-81 0.5060575283592996 1102 82-83 0.5763043754691708 1102 84-85 0.5135086736259353 1102 86-87 0.5510258889566302 1102 88-89 0.5438855177501694 1102 90-91 0.5701231721595832 1102 92-93 0.6129127457051027 1102 94-95 0.5799105981063022 1102 96-97 0.5500048741239709 1102 98-99 0.5965130676214372 1102 100 0.5769921807932015 1103 1 0.46627981458722445 1103 2 3.959028364423471 1103 3 2.254814044477019 1103 4 1.0506754225810937 1103 5 0.41605185109868614 1103 6 0.41087649462138387 1103 7 0.3054873361068786 1103 8 0.3368295218493742 1103 9 0.43490181973270836 1103 10-11 0.43190217072465487 1103 12-13 0.449563660467831 1103 14-15 0.6805298346000797 1103 16-17 0.6103698693869433 1103 18-19 0.6344465016706096 1103 20-21 0.6653599900542773 1103 22-23 0.5642309007576145 1103 24-25 0.5381999845600589 1103 26-27 0.5887835287310921 1103 28-29 0.6347871327871317 1103 30-31 0.4870833274235764 1103 32-33 0.6707634902002226 1103 34-35 0.7573131956412524 1103 36-37 0.6580507526685224 1103 38-39 0.5580928721433978 1103 40-41 0.592757601167186 1103 42-43 0.5994029239730096 1103 44-45 0.6207737959483595 1103 46-47 0.6747738506286893 1103 48-49 0.6583076720303467 1103 50-51 0.5109566276121527 1103 52-53 0.5055752608843846 1103 54-55 0.6318700330970373 1103 56-57 0.5355678112282121 1103 58-59 0.5378131938582413 1103 60-61 0.5663868145563669 1103 62-63 0.629793499647505 1103 64-65 0.6218323046992182 1103 66-67 0.49361861224619474 1103 68-69 0.5193558600118777 1103 70-71 0.5766681515984686 1103 72-73 0.5778687386055807 1103 74-75 0.5010861661500527 1103 76-77 0.4006929458930344 1103 78-79 0.4512722900581707 1103 80-81 0.5073434963951158 1103 82-83 0.49612558189245703 1103 84-85 0.4655647417787776 1103 86-87 0.5243732235696683 1103 88-89 0.47208636668739246 1103 90-91 0.4462813476700447 1103 92-93 0.49157815823293305 1103 94-95 0.40726964574573543 1103 96-97 0.4838780996572609 1103 98-99 0.5353675405382177 1103 100 0.5702237855676096 1104 1 0.3168822018944617 1104 2 3.542439544731895 1104 3 2.158946730321226 1104 4 0.8553190564284634 1104 5 0.3951987471050131 1104 6 0.36731678722716055 1104 7 0.28785499624923006 1104 8 0.2786895702874901 1104 9 0.2866125996852631 1104 10-11 0.3137344784549754 1104 12-13 0.3787769735362403 1104 14-15 0.45971009359573145 1104 16-17 0.5424122403833849 1104 18-19 0.5383008536872183 1104 20-21 0.5614972972903374 1104 22-23 0.4686094841897983 1104 24-25 0.4526152672804997 1104 26-27 0.45316761087817525 1104 28-29 0.5027348764605222 1104 30-31 0.5125992180918217 1104 32-33 0.5690879267364011 1104 34-35 0.6916644090655311 1104 36-37 0.5433611520358639 1104 38-39 0.48990015763173034 1104 40-41 0.4548306784704721 1104 42-43 0.5202989673696123 1104 44-45 0.550507324516964 1104 46-47 0.5923138244325585 1104 48-49 0.4289553465064202 1104 50-51 0.43162438166513795 1104 52-53 0.3142842367641805 1104 54-55 0.45199440290723913 1104 56-57 0.39603853829424196 1104 58-59 0.4891826210033514 1104 60-61 0.4806192186686644 1104 62-63 0.4571517687261917 1104 64-65 0.4318912459803599 1104 66-67 0.4485205003442587 1104 68-69 0.4015693707868948 1104 70-71 0.35640446041618645 1104 72-73 0.36557540378944964 1104 74-75 0.4289513673165217 1104 76-77 0.40619783913147245 1104 78-79 0.3528873058746953 1104 80-81 0.3707421346908788 1104 82-83 0.3935625011293027 1104 84-85 0.4321764212963686 1104 86-87 0.5173673047282392 1104 88-89 0.4386424412227825 1104 90-91 0.35542311566135254 1104 92-93 0.3872048263823942 1104 94-95 0.3893407459829845 1104 96-97 0.40509078253586495 1104 98-99 0.3724761432468 1104 100 0.32179617089778034 1105 1 0.4224658010387614 1105 2 3.7063941655755315 1105 3 2.150224386323451 1105 4 0.9020705566942198 1105 5 0.36633864159222895 1105 6 0.1938025413699478 1105 7 0.20018045680869534 1105 8 0.21819153790572443 1105 9 0.3215301517435236 1105 10-11 0.3183619582155899 1105 12-13 0.26067270514445084 1105 14-15 0.3943178814857973 1105 16-17 0.41066895133897674 1105 18-19 0.45791095716053576 1105 20-21 0.3869320966786205 1105 22-23 0.34695639621206453 1105 24-25 0.3955780434953837 1105 26-27 0.4050871917387866 1105 28-29 0.36484179992892507 1105 30-31 0.35893515274342747 1105 32-33 0.4618974759757961 1105 34-35 0.492947235909476 1105 36-37 0.32648861391188433 1105 38-39 0.3809099213606615 1105 40-41 0.30470272523897535 1105 42-43 0.36776298013104736 1105 44-45 0.3737250470749487 1105 46-47 0.3694757728107376 1105 48-49 0.31760315256973826 1105 50-51 0.27497985004989545 1105 52-53 0.20167138345731672 1105 54-55 0.2721971450849239 1105 56-57 0.3010044289372189 1105 58-59 0.28900026097847586 1105 60-61 0.26178163518372344 1105 62-63 0.33151347726266067 1105 64-65 0.24673504451582318 1105 66-67 0.2475343066171689 1105 68-69 0.2327158794333002 1105 70-71 0.2883170254988201 1105 72-73 0.25140152419601236 1105 74-75 0.22643205667004906 1105 76-77 0.14402800584718278 1105 78-79 0.2486647864824718 1105 80-81 0.25550989549677894 1105 82-83 0.30548522904953046 1105 84-85 0.26004081032439785 1105 86-87 0.32004894152554897 1105 88-89 0.2309630039157824 1105 90-91 0.21936625271814592 1105 92-93 0.24195356049884964 1105 94-95 0.1624341706678578 1105 96-97 0.2301261281256899 1105 98-99 0.23518929142969114 1105 100 0.3362835327707252 1106 1 0.501092788072075 1106 2 3.950706868211352 1106 3 2.276543053315404 1106 4 1.0884042735911947 1106 5 0.44592085362393874 1106 6 0.4049066398234018 1106 7 0.3722486997432455 1106 8 0.3204745660412982 1106 9 0.3639027667024095 1106 10-11 0.3583897022903102 1106 12-13 0.3071946579425813 1106 14-15 0.412653808716243 1106 16-17 0.4223371989323965 1106 18-19 0.4310610850039467 1106 20-21 0.4217461169482135 1106 22-23 0.3454730093434719 1106 24-25 0.3563916670095537 1106 26-27 0.35449138857956797 1106 28-29 0.3594103177618848 1106 30-31 0.3080567333579154 1106 32-33 0.376699806488098 1106 34-35 0.5047524317523582 1106 36-37 0.44693175014327124 1106 38-39 0.3426237275726862 1106 40-41 0.41849939409648584 1106 42-43 0.4308403450588756 1106 44-45 0.38226566779179194 1106 46-47 0.4073219882549495 1106 48-49 0.2637448563737834 1106 50-51 0.26827394137477256 1106 52-53 0.28144134358640116 1106 54-55 0.31621544029400894 1106 56-57 0.34589175377871584 1106 58-59 0.3801111736562177 1106 60-61 0.3273499616523239 1106 62-63 0.4040102767939686 1106 64-65 0.3251526171992154 1106 66-67 0.18895675934216172 1106 68-69 0.2129855159462224 1106 70-71 0.2925101654873572 1106 72-73 0.21034979921164876 1106 74-75 0.29469806640258156 1106 76-77 0.3266517527112214 1106 78-79 0.2623261884167505 1106 80-81 0.22431595535976356 1106 82-83 0.3224829051247795 1106 84-85 0.34533312877372424 1106 86-87 0.345670177483818 1106 88-89 0.32836398663688726 1106 90-91 0.2527345515841901 1106 92-93 0.3562798627783863 1106 94-95 0.4221193143063431 1106 96-97 0.25785024296533976 1106 98-99 0.3920417040483244 1106 100 0.40055443582012984 1107 1 0.30292710313557336 1107 2 3.513114103316319 1107 3 2.0456416113971123 1107 4 0.8116345775664442 1107 5 0.18925114625984207 1107 6 0.07506137622429776 1107 7 0.05973604423232359 1107 8 0.043083357875353556 1107 9 0.03146907145520572 1107 10-11 0.04784611772314662 1107 12-13 0.021435752512651618 1107 14-15 -0.08165709618315731 1107 16-17 -0.10189013150400683 1107 18-19 0.029121045802234846 1107 20-21 0.020582430063775803 1107 22-23 0.022542084134499873 1107 24-25 0.04006850043872845 1107 26-27 0.08666065958164637 1107 28-29 0.0036500480678824943 1107 30-31 -0.025505643630516772 1107 32-33 0.04200665959912442 1107 34-35 0.1949238835520717 1107 36-37 0.01527733063637271 1107 38-39 -0.08990373235490523 1107 40-41 0.012073229384498063 1107 42-43 0.13731975150528086 1107 44-45 0.10580989171536004 1107 46-47 0.04400947914297859 1107 48-49 -0.08239349164669107 1107 50-51 -0.1372174240232411 1107 52-53 -0.07844905209574193 1107 54-55 -0.07898598228666032 1107 56-57 -0.04050497674597153 1107 58-59 0.06735325434370054 1107 60-61 0.08809278192786962 1107 62-63 0.16249067138267748 1107 64-65 0.07217420598217927 1107 66-67 0.06144672858420108 1107 68-69 0.001516421210105534 1107 70-71 0.01809401482805839 1107 72-73 -0.012960993813045718 1107 74-75 0.06682432891634704 1107 76-77 0.01296352679110413 1107 78-79 0.023211636300345617 1107 80-81 0.13665116795209542 1107 82-83 0.13201822513708805 1107 84-85 0.07580928224761152 1107 86-87 0.048490875723786075 1107 88-89 0.034311039016522216 1107 90-91 0.05292751015915087 1107 92-93 0.13398783838261608 1107 94-95 -0.03458523367930155 1107 96-97 -0.0015665328761969022 1107 98-99 0.01135197617217898 1107 100 0.04173362064323882 1108 1 0.38715666768695556 1108 2 3.61241729074872 1108 3 2.062276514527934 1108 4 0.8413556412094252 1108 5 0.1669465800594523 1108 6 -0.04662200792429161 1108 7 -0.06091400037110617 1108 8 -0.09346257533540836 1108 9 -0.05003612950358871 1108 10-11 0.01695547073963155 1108 12-13 -0.024220359822678006 1108 14-15 -0.09922739061705244 1108 16-17 -0.10193569552770043 1108 18-19 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0.9937932571466135 2211 5 0.46572103571241996 2211 6 0.20982511778649382 2211 7 0.25335126651511075 2211 8 0.21403356261868822 2211 9 0.1034122464443783 2211 10-11 0.1357494900485534 2211 12-13 0.11264786101963153 2211 14-15 0.03727572284608982 2211 16-17 -0.06786989475642002 2211 18-19 0.11324225665293852 2211 20-21 0.12722715495301884 2211 22-23 0.05698159051438978 2211 24-25 0.03395764554795022 2211 26-27 -0.010672785556920417 2211 28-29 -0.06363923400274984 2211 30-31 -0.10735056976918145 2211 32-33 0.048033660563440606 2211 34-35 0.18477581149360134 2211 36-37 -0.02862019986550024 2211 38-39 -0.11990422150280722 2211 40-41 -0.057034634716238486 2211 42-43 0.06323480747777666 2211 44-45 0.117376492696458 2211 46-47 0.21411066288090552 2211 48-49 0.08238646167462349 2211 50-51 0.10626293319449331 2211 52-53 -0.004512599396342409 2211 54-55 0.010031948477937647 2211 56-57 -0.1511310161852677 2211 58-59 -0.06507131206161887 2211 60-61 0.004500898987956248 2211 62-63 0.1366182411561283 2211 64-65 -0.16864553602723475 2211 66-67 -0.09080929142893979 2211 68-69 0.015790805233137917 2211 70-71 0.023857225315381925 2211 72-73 -0.09963974942078124 2211 74-75 -0.16497587512820644 2211 76-77 -0.05287403417768388 2211 78-79 -0.06299194705744071 2211 80-81 -0.08374315513707842 2211 82-83 -0.3928916318919633 2211 84-85 -0.2086284771754734 2211 86-87 -0.02603954228803218 2211 88-89 0.05652883561484856 2211 90-91 0.05574884539318248 2211 92-93 0.1485626989754607 2211 94-95 0.0484476230842148 2211 96-97 0.0308709610094553 2211 98-99 -0.07328982780529003 2211 100 -0.23216474839789214 2212 1 0.5579712828411942 2212 2 4.03050653902665 2212 3 2.20875749685797 2212 4 0.9912508194064884 2212 5 0.7690280415748703 2212 6 0.4563130025578914 2212 7 0.2918712646783064 2212 8 0.22628884724620235 2212 9 -0.019488061219668396 2212 10-11 0.25287191278814447 2212 12-13 0.30384193427735084 2212 14-15 0.4143095965048431 2212 16-17 0.3721134209742445 2212 18-19 0.39837259294045424 2212 20-21 0.5049433233876073 2212 22-23 0.3987795118687174 2212 24-25 0.2805610956711746 2212 26-27 0.2772383898422035 2212 28-29 0.27423405739030215 2212 30-31 0.2100247956775405 2212 32-33 0.359057140993869 2212 34-35 0.3474049615142647 2212 36-37 0.5060666256843902 2212 38-39 0.3284797769053043 2212 40-41 0.41088756148464967 2212 42-43 0.36454429302063573 2212 44-45 0.46488341896422725 2212 46-47 0.4563834736445571 2212 48-49 0.31397483472876075 2212 50-51 0.3824844053899241 2212 52-53 0.3484324037415263 2212 54-55 0.2946552513510028 2212 56-57 0.4152429104345643 2212 58-59 0.28839603115982015 2212 60-61 0.3795812590008154 2212 62-63 0.45417345996496294 2212 64-65 0.5589106777150832 2212 66-67 0.49763646938905026 2212 68-69 0.3654992131864816 2212 70-71 0.45003124683655926 2212 72-73 0.3606440362246275 2212 74-75 0.48844975741989316 2212 76-77 0.3432028665279532 2212 78-79 0.461044115454996 2212 80-81 0.2960711749665492 2212 82-83 0.42639591919404296 2212 84-85 0.616184781461314 2212 86-87 0.3115632037283973 2212 88-89 0.5427956920842192 2212 90-91 0.3725586294160763 2212 92-93 0.26277111458214364 2212 94-95 0.4976218282854177 2212 96-97 0.39900706791122786 2212 98-99 0.42310315561758216 2212 100 0.26345295223427456 2213 1 -0.45764040899000946 2213 2 -26.010763302243195 2213 3 -14.878853807826504 2213 4 -6.053880810481239 2213 5 -2.0304251136709652 2213 6 -1.1297400365439287 2213 7 -1.1409684565760472 2213 8 -1.115808520156044 2213 9 -1.0345722656331446 2213 10-11 -0.9625825006845616 2213 12-13 -0.8703910312665855 2213 14-15 -1.3060131870801328 2213 16-17 -1.1751039619138695 2213 18-19 -1.2519927767846752 2213 20-21 -1.3144672457180846 2213 22-23 -1.2291845802721966 2213 24-25 -1.237037142811218 2213 26-27 -1.2907832903745984 2213 28-29 -1.327520201223706 2213 30-31 -1.2126329666143647 2213 32-33 -1.0197852902875866 2213 34-35 -1.1329666993451966 2213 36-37 -1.2037548028870404 2213 38-39 -1.2037380899162216 2213 40-41 -1.3375057795916234 2213 42-43 -1.1381245997397258 2213 44-45 -1.1929224269513767 2213 46-47 -1.0907855159024749 2213 48-49 -1.1372737134710107 2213 50-51 -1.1922615295694285 2213 52-53 -1.4396652540207668 2213 54-55 -1.1976332697484935 2213 56-57 -1.2063381000184066 2213 58-59 -1.1838987810740136 2213 60-61 -1.1229593914056935 2213 62-63 -1.2280782821953125 2213 64-65 -1.1712190164389966 2213 66-67 -1.0677550327402514 2213 68-69 -1.1678413791480082 2213 70-71 -1.1534216180492294 2213 72-73 -1.2326559057033606 2213 74-75 -1.262508430733412 2213 76-77 -1.2033466882533084 2213 78-79 -1.134203658451316 2213 80-81 -1.0356337224395737 2213 82-83 -1.1524368334191983 2213 84-85 -1.0186027717713628 2213 86-87 -1.0658177486525489 2213 88-89 -1.240714276944047 2213 90-91 -1.0490925513814453 2213 92-93 -1.0971858153443037 2213 94-95 -1.1955885859615805 2213 96-97 -1.223430504678781 2213 98-99 -1.2186361010606959 2213 100 -1.2092435247304962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 27.0 5 41.0 6 34.0 7 29.0 8 51.0 9 84.0 10 303.0 11 1372.0 12 3789.0 13 6984.0 14 9471.0 15 10882.0 16 12144.0 17 12436.0 18 12836.0 19 13963.0 20 15724.0 21 18271.0 22 20481.0 23 21835.0 24 22107.0 25 23246.0 26 25479.0 27 29097.0 28 34516.0 29 41269.0 30 50828.0 31 63030.0 32 80068.0 33 108514.0 34 151311.0 35 219357.0 36 341902.0 37 595308.0 38 622124.0 39 4820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.83511507691345 20.87459236261545 10.718145631143887 29.572146929327214 2 12.67318934470551 22.72095656875848 37.224236612016284 27.381617474519725 3 15.805825061651502 27.418337742608912 28.45771971616414 28.31811747957545 4 10.582305395015073 18.651708619605703 37.74983505582457 33.016150929554655 5 11.703681158765978 38.67571381755881 35.12812948313078 14.492475540544433 6 29.84211796968942 39.52446839074316 17.795832965624864 12.837580673942565 7 25.75375190019446 33.05897200791523 23.42221183995572 17.765064251934593 8 24.131388569387266 37.3500485698615 20.863466795823495 17.65509606492774 9 24.43389930122446 17.09592042113888 22.087557426351033 36.382622851285625 10-11 22.903625742223646 28.0256807292105 28.981429419101097 20.08926410946476 12-13 23.845987151343415 25.419563736508277 29.55074403069995 21.183705081448355 14-15 21.496082014818317 26.762626870243427 26.671064362165385 25.07022675277287 16-17 20.59472335192443 30.47310030427625 27.694988371738244 21.23718797206108 18-19 20.63597339277143 29.32434507002567 29.804079697316542 20.235601839886357 20-21 21.77201469654373 28.65225589769937 28.972567177149006 20.603162228607893 22-23 20.780835376251982 28.694061985545932 29.187121762097007 21.33798087610508 24-25 21.030523107821473 28.460149588177824 28.980358293454294 21.528969010546408 26-27 21.031471704066735 28.374895225793516 29.057263354072504 21.536369716067245 28-29 20.683762942386437 28.35701497455922 29.550067599838492 21.409154483215847 30-31 21.47451536372909 28.761339238296994 28.76961722474092 20.994528173232997 32-33 20.311946628009107 28.75217657626084 29.57940668906106 21.356470106668993 34-35 20.4690273584398 28.33733508207066 29.65858241539842 21.535055144091125 36-37 21.25632931250499 28.72699197887195 28.901861001954238 21.114817706668823 38-39 21.022073230319098 28.676004456805032 29.146008934980966 21.155913377894908 40-41 21.113346236356918 28.543548870298967 28.988205817862205 21.35489907548191 42-43 20.93437444143087 28.59895319287829 29.185849063935283 21.280823301755557 44-45 21.434536033401788 28.105315104050298 28.97167881282992 21.488470049717993 46-47 21.209418040019113 28.789814461650444 28.987557006181376 21.013210492149074 48-49 21.210495991667567 28.583884472970595 28.977304414655215 21.228315120706625 50-51 21.31853569010059 28.560455393810912 28.618535787257482 21.502473128831017 52-53 20.799892265690996 28.801904705272623 28.587485447415105 21.810717581621272 54-55 21.229304847291967 28.728460048039395 28.31364855534956 21.72858654931908 56-57 21.436621053789207 28.460787860676646 28.349317205807434 21.75327387972672 58-59 20.922757913708622 28.146296404959912 29.04918528409067 21.881760397240797 60-61 21.555205179095154 28.545351569748068 28.33057921891955 21.568864032237226 62-63 20.745477918688422 28.832353642963675 28.731431156252256 21.690737282095643 64-65 20.669089953799247 28.28693859634976 28.988975513815046 22.054995936035944 66-67 21.198178530796802 28.48310322079825 28.545146651199527 21.77357159720542 68-69 21.38543277333541 28.352063949867386 28.579948313564373 21.682554963232832 70-71 21.373705064700612 28.391472227998005 28.329035505177796 21.905787202123587 72-73 21.11133824777622 28.59766988872549 28.70602185071991 21.58497001277838 74-75 21.564719322474314 28.017404122742946 28.389644504217436 22.028232050565308 76-77 21.22750971971054 28.588336036464383 28.62469083363629 21.559463410188787 78-79 21.34194288481114 28.501480661248685 28.694925492846174 21.461650961094 80-81 21.333864925469268 28.354261859255153 28.43112510814966 21.880748107125918 82-83 20.734472804506492 28.700292555020667 28.551081334492313 22.014153305980532 84-85 20.975393571323515 28.23818265999366 28.398337098377723 22.3880866703051 86-87 21.279869283331486 28.46604144930746 28.249002819112455 22.005086448248594 88-89 20.956157279152954 28.096581708195856 28.762995417033622 22.184265595617575 90-91 21.284620911246048 28.247679375555894 28.404330511164915 22.063369202033144 92-93 20.621962961437525 28.675666623407608 28.588708142002655 22.113662273152208 94-95 20.57395344192752 28.138304599526638 28.802384728196895 22.485357230348946 96-97 20.99884913755254 28.30587375015639 28.496473201068333 22.198803911222733 98-99 20.939947445904366 28.148340404137347 28.526391084686846 22.38532106527144 100 20.93999407199031 28.180101025110023 28.428462848416437 22.45144205448323 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7.0 1 6.5 2 6.0 3 9.0 4 13.0 5 20.5 6 50.5 7 96.5 8 138.5 9 177.0 10 185.5 11 183.0 12 221.0 13 271.5 14 334.0 15 429.0 16 511.5 17 610.5 18 830.5 19 1108.0 20 1477.0 21 2071.5 22 2934.5 23 4289.0 24 6169.5 25 8925.5 26 13014.5 27 18596.0 28 25819.5 29 34058.0 30 43007.5 31 52780.0 32 62411.5 33 71719.0 34 80160.0 35 87635.5 36 94504.0 37 100546.5 38 105477.0 39 108315.5 40 109412.5 41 108855.0 42 106329.0 43 102297.5 44 97569.5 45 92486.0 46 88314.5 47 85326.5 48 81595.5 49 80364.0 50 91563.0 51 87818.5 52 66709.5 53 57237.0 54 52951.0 55 48852.0 56 44847.5 57 40769.0 58 36423.0 59 32089.5 60 27643.5 61 23039.5 62 18779.5 63 15117.0 64 11882.5 65 9328.0 66 7203.0 67 5305.0 68 3933.5 69 2940.5 70 2205.0 71 1650.5 72 1245.5 73 916.0 74 609.0 75 400.5 76 252.0 77 150.0 78 90.5 79 57.0 80 28.5 81 15.0 82 8.5 83 4.0 84 2.5 85 2.0 86 0.5 87 0.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6341352917795535 2 4.791313500464887 3 0.0 4 0.005556114620701927 5 0.17045227161551998 6 0.05843633838836153 7 0.0379991615317936 8 0.04499287224316666 9 0.04266163533937564 10-11 0.04470146763019278 12-13 0.0426810623135739 14-15 0.04590594003048481 16-17 0.03553193580861477 18-19 0.029373584987766835 20-21 0.024050594057444006 22-23 0.02428371774782311 24-25 0.021097693979308718 26-27 0.013598881938780942 28-29 0.01981551368222366 30-31 0.025313347380330812 32-33 0.0012238993744902848 34-35 0.0028946191555405147 36-37 0.0039048218138499563 38-39 0.0057698113368827705 40-41 0.0071491265049591236 42-43 0.007809643627699913 44-45 0.008780992337612837 46-47 0.017309434010648314 48-49 0.025779594761089014 50-51 0.022671278889367655 52-53 0.028926764581206885 54-55 0.02504136974155519 56-57 0.016240950429744095 58-59 0.015153039874641621 60-61 0.012374982564290657 62-63 0.018611041281931633 64-65 0.019990356450007983 66-67 0.021117120953506974 68-69 0.028188539561673068 70-71 0.028596506019836494 72-73 0.025060796715753446 74-75 0.02612928029665767 76-77 0.019057861688491575 78-79 0.015444444487615498 80-81 0.013190915480617514 82-83 0.00808162126647553 84-85 0.01117051016399863 86-87 0.009169531821578007 88-89 0.007362823221139967 90-91 0.007304542298545191 92-93 0.0038465408912551806 94-95 0.0011850454260937677 96-97 7.382250195338226E-4 98-99 4.662473807582037E-4 100 0.11248218060791665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2573741.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.91770893996028 #Duplication Level Percentage of deduplicated Percentage of total 1 91.99393432286493 79.95902007716596 2 6.333389280135939 11.009673721086402 3 0.9885175115681712 2.577590320576084 4 0.28011641281364263 0.9738830735296781 5 0.13817775600030413 0.6005046989005643 6 0.06969205860018016 0.36344844389022696 7 0.04318327693048185 0.2627374046722126 8 0.031815560110891705 0.22122684747842367 9 0.023762750921883402 0.18588634814169308 >10 0.0900214214171715 1.3187278917734653 >50 0.003567614624743509 0.2127463361130997 >100 0.0023416195654215022 0.36595905880884916 >500 8.97220876540103E-4 0.521403584735477 >1k 4.934714820309562E-4 0.8221113654966323 >5k 8.972208764199202E-5 0.6050808276312246 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8577 0.3332503153969261 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6941 0.26968525582022435 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2884 0.11205478717555496 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9426974198258488E-5 0.0 12-13 3.8853948396516975E-5 0.0 0.0 3.8853948396516975E-5 0.0 14-15 3.8853948396516975E-5 0.0 0.0 3.8853948396516975E-5 0.0 16-17 3.8853948396516975E-5 0.0 0.0 3.8853948396516975E-5 0.0 18-19 3.8853948396516975E-5 0.0 0.0 5.828092259477546E-5 0.0 20-21 7.770789679303395E-5 0.0 0.0 1.554157935860679E-4 0.0 22-23 7.770789679303395E-5 0.0 0.0 2.7197763877561884E-4 0.0 24-25 7.770789679303395E-5 0.0 0.0 4.6624738075820376E-4 0.0 26-27 7.770789679303395E-5 0.0 0.0 6.022362001460132E-4 0.0 28-29 7.770789679303395E-5 0.0 0.0 0.0010102026583094414 0.0 30-31 7.770789679303395E-5 0.0 0.0 0.0030111810007300657 0.0 32-33 7.770789679303395E-5 0.0 0.0 0.00810104824067379 0.0 34-35 7.770789679303395E-5 0.0 0.0 0.01503647802945207 0.0 36-37 7.770789679303395E-5 0.0 0.0 0.027411460593742727 1.9426974198258488E-5 38-39 7.770789679303395E-5 0.0 0.0 0.04580880515949351 3.8853948396516975E-5 40-41 7.770789679303395E-5 0.0 0.0 0.07123871438501388 3.8853948396516975E-5 42-43 7.770789679303395E-5 0.0 0.0 0.09515331962307008 3.8853948396516975E-5 44-45 7.770789679303395E-5 0.0 0.0 0.12481830922381079 3.8853948396516975E-5 46-47 7.770789679303395E-5 0.0 0.0 0.162487212194234 3.8853948396516975E-5 48-49 7.770789679303395E-5 7.770789679303395E-5 0.0 0.2021182395586813 3.8853948396516975E-5 50-51 7.770789679303395E-5 7.770789679303395E-5 0.0 0.24501299858843606 3.8853948396516975E-5 52-53 7.770789679303395E-5 7.770789679303395E-5 0.0 0.2929976248581345 3.8853948396516975E-5 54-55 9.713487099129243E-5 7.770789679303395E-5 0.0 0.3437214544897874 3.8853948396516975E-5 56-57 1.1656184518955093E-4 7.770789679303395E-5 0.0 0.4037896587108027 3.8853948396516975E-5 58-59 1.1656184518955093E-4 7.770789679303395E-5 0.0 0.4735713500309472 3.8853948396516975E-5 60-61 1.1656184518955093E-4 7.770789679303395E-5 0.0 0.551201538927188 3.8853948396516975E-5 62-63 1.1656184518955093E-4 7.770789679303395E-5 0.0 0.6321343134371329 3.8853948396516975E-5 64-65 1.1656184518955093E-4 7.770789679303395E-5 0.0 0.7205464730134074 3.8853948396516975E-5 66-67 1.1656184518955093E-4 7.770789679303395E-5 0.0 0.8227323572962469 3.8853948396516975E-5 68-69 1.1656184518955093E-4 7.770789679303395E-5 0.0 0.9427910578414844 3.8853948396516975E-5 70-71 1.1656184518955093E-4 7.770789679303395E-5 0.0 1.083461778011074 3.8853948396516975E-5 72-73 1.1656184518955093E-4 7.770789679303395E-5 0.0 1.2234914080321213 3.8853948396516975E-5 74-75 1.1656184518955093E-4 7.770789679303395E-5 0.0 1.3673675789444237 3.8853948396516975E-5 76-77 1.1656184518955093E-4 7.770789679303395E-5 0.0 1.5293885437578996 3.8853948396516975E-5 78-79 1.1656184518955093E-4 7.770789679303395E-5 0.0 1.7156155184224053 3.8853948396516975E-5 80-81 1.1656184518955093E-4 7.770789679303395E-5 0.0 1.9221631080982897 3.8853948396516975E-5 82-83 1.1656184518955093E-4 7.770789679303395E-5 0.0 2.14749658182389 3.8853948396516975E-5 84-85 1.554157935860679E-4 7.770789679303395E-5 0.0 2.375297281272669 3.8853948396516975E-5 86-87 1.554157935860679E-4 7.770789679303395E-5 0.0 2.6203879877578977 3.8853948396516975E-5 88 1.554157935860679E-4 7.770789679303395E-5 0.0 2.829927331460314 3.8853948396516975E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 165 9.249743E-8 25.65842 5 TACACCG 215 1.1853572E-6 19.691347 5 GAGTACT 3775 0.0 17.538656 12-13 GTCTCGC 430 3.6379788E-11 17.251207 1 GTATAGA 1190 0.0 15.791862 1 TACGACC 180 0.002795265 15.680148 4 CGTAGAC 210 4.3135553E-4 15.679842 3 GTATAGG 735 0.0 15.475233 1 GACCGCG 185 0.0033948468 15.229375 7 AGTACTT 4455 0.0 15.072401 12-13 TCCGACG 110 6.23329E-4 14.938473 30-31 GGACCGA 190 0.00405126 14.830043 6 GTGTATA 1860 0.0 14.357457 1 GTCTTAG 865 0.0 14.292909 1 CTAGTAC 630 0.0 14.1865225 3 CTACACT 1175 0.0 14.012047 4 GTACAGG 1245 0.0 13.90258 1 GTACTTT 4870 0.0 13.835535 14-15 CATGGGG 4105 0.0 13.751164 4 GAGTGCG 280 2.5180975E-4 13.416354 7 >>END_MODULE