FastQCFastQC Report
Wed 25 May 2016
SRR1295149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776620
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65820.23705080277459648No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52350.18853858288134492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG41850.016.3011044
GGATCGT1900.002666159515.7916546
CTATGCG3552.8637987E-715.5169919
GTATAGA11200.015.4355261
TCGACAC2907.684677E-515.4260593
ATACCGA2701.0835746E-414.816866
GTGTATA13000.014.7231171
GTCTTAG8850.014.6506671
TATACTG14050.014.5974445
CTCGTAT3255.9702506E-714.54276294
GTACATG215050.014.2404471
GTACATA14000.014.1124811
GTATATA18150.013.9472521
GTATAAG9300.013.9417641
GTCCTAA8005.456968E-1213.8919731
TACATGG217800.013.77498152
GTATTAA15450.013.5872581
CTGTGCG5902.4556357E-1013.5803559
TCTAGAC8400.013.31415753
CTATAGT8950.013.1204544
Produced by FastQC (version 0.11.5)