##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295149_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2776620 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.291176322291133 33.0 27.0 33.0 6.0 33.0 2 22.42262354949543 27.0 6.0 33.0 2.0 33.0 3 24.662950637825844 27.0 15.0 33.0 15.0 33.0 4 24.865635917050227 33.0 6.0 37.0 2.0 37.0 5 26.563756653773293 33.0 15.0 37.0 2.0 37.0 6 27.540348337186938 33.0 15.0 37.0 6.0 37.0 7 27.169985810085645 33.0 15.0 37.0 6.0 37.0 8 28.01740245334255 33.0 22.0 37.0 6.0 37.0 9 29.02016768589148 33.0 27.0 37.0 15.0 37.0 10-11 28.575170170927244 33.0 24.5 37.0 10.5 37.0 12-13 27.394300804575344 33.0 15.0 37.0 4.0 37.0 14-15 29.148230402431732 33.0 22.0 37.0 6.0 40.0 16-17 29.548298110652517 33.0 27.0 37.0 6.0 40.0 18-19 29.701668935612364 33.0 27.0 38.5 10.5 40.0 20-21 29.22729523665464 33.0 22.0 38.5 6.0 40.0 22-23 29.944148100928466 33.0 27.0 37.0 15.0 40.0 24-25 30.091959828856666 33.0 27.0 37.0 15.0 40.0 26-27 28.71340280628966 33.0 18.5 37.0 4.0 40.0 28-29 29.38123077698785 33.0 22.0 37.0 6.0 40.0 30-31 30.056180536047425 33.0 27.0 37.0 15.0 40.0 32-33 28.842824189122027 33.0 22.0 37.0 6.0 40.0 34-35 28.48765675533562 33.0 22.0 37.0 6.0 40.0 36-37 28.76345142655459 33.0 22.0 37.0 6.0 40.0 38-39 28.885382227312345 33.0 22.0 37.0 6.0 40.0 40-41 27.949141402136412 33.0 22.0 37.0 6.0 40.0 42-43 28.519249663259647 33.0 22.0 37.0 6.0 40.0 44-45 28.396283250858957 33.0 22.0 37.0 6.0 40.0 46-47 28.056984931319374 33.0 22.0 37.0 6.0 40.0 48-49 27.92757651389099 33.0 22.0 37.0 6.0 40.0 50-51 27.886788253343994 33.0 22.0 37.0 6.0 40.0 52-53 28.0816143368556 33.0 22.0 37.0 6.0 40.0 54-55 28.051464730499674 33.0 22.0 37.0 6.0 40.0 56-57 28.009452499801917 33.0 22.0 37.0 6.0 40.0 58-59 27.884254777391217 33.0 22.0 37.0 6.0 40.0 60-61 27.84068165611427 33.0 22.0 37.0 6.0 40.0 62-63 27.78478996045552 33.0 22.0 37.0 6.0 40.0 64-65 27.341898783412926 33.0 22.0 37.0 6.0 40.0 66-67 27.515580814083307 33.0 22.0 37.0 6.0 37.0 68-69 27.103744840849664 33.0 22.0 37.0 6.0 37.0 70-71 27.204242388227414 33.0 22.0 37.0 6.0 37.0 72-73 27.234557663634202 27.0 22.0 37.0 10.5 37.0 74-75 27.32713947173182 27.0 22.0 37.0 10.5 37.0 76-77 27.070060541233588 27.0 22.0 35.0 6.0 37.0 78-79 26.674683247977757 27.0 22.0 33.0 6.0 37.0 80-81 26.490308720674776 27.0 22.0 33.0 6.0 37.0 82-83 26.596775936210214 27.0 22.0 33.0 10.5 37.0 84-85 26.534306819082193 27.0 22.0 33.0 10.5 37.0 86-87 26.28229357996413 27.0 22.0 33.0 10.5 37.0 88-89 26.226388018526123 27.0 22.0 33.0 6.0 37.0 90-91 26.377784500579843 27.0 22.0 33.0 10.5 37.0 92-93 26.28773130640851 27.0 22.0 33.0 6.0 37.0 94-95 26.166696559125846 27.0 22.0 33.0 10.5 37.0 96-97 26.26338479878413 27.0 22.0 33.0 10.5 37.0 98-99 26.307802832220467 27.0 22.0 33.0 10.5 37.0 100 25.969937910120937 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.164779528770374 1101 2 11.16685951244115 1101 3 8.288093135342379 1101 4 12.713397943233666 1101 5 10.602354318526004 1101 6 9.891027972833278 1101 7 9.354186383458586 1101 8 8.93698602750629 1101 9 7.319895989188691 1101 10-11 7.691209478109204 1101 12-13 9.02943875794999 1101 14-15 7.829807020113837 1101 16-17 8.064166879457083 1101 18-19 8.003659612084125 1101 20-21 7.143134541023631 1101 22-23 7.338187484777755 1101 24-25 7.207511058636491 1101 26-27 8.426620896231139 1101 28-29 8.14739967660163 1101 30-31 5.383604857018437 1101 32-33 6.758536696729106 1101 34-35 8.684549074104737 1101 36-37 8.135618925659948 1101 38-39 8.176386778623716 1101 40-41 8.97589241801342 1101 42-43 8.532011668890352 1101 44-45 8.355677815525691 1101 46-47 7.223463058053195 1101 48-49 5.196695326525745 1101 50-51 6.60765490159487 1101 52-53 5.783553108107949 1101 54-55 3.941704958362095 1101 56-57 6.07057164022774 1101 58-59 5.875045406175719 1101 60-61 6.427494547791916 1101 62-63 5.903515270658236 1101 64-65 6.516656429914185 1101 66-67 5.600254761359274 1101 68-69 6.506692846646356 1101 70-71 6.1333114738130305 1101 72-73 5.307111364926289 1101 74-75 4.590447220876879 1101 76-77 4.71492464132211 1101 78-79 4.7446342655836595 1101 80-81 4.693980537686258 1101 82-83 4.192204214400043 1101 84-85 3.950981714331153 1101 86-87 3.884642280174031 1101 88-89 3.8286181847664764 1101 90-91 3.685254240018512 1101 92-93 3.5588872730280983 1101 94-95 3.4622905160611097 1101 96-97 3.5534610084719276 1101 98-99 3.2276246863031126 1101 100 3.441387269994401 1102 1 6.69428659837012 1102 2 10.331259193016727 1102 3 8.011273498664867 1102 4 12.353100935810666 1102 5 10.4040063395123 1102 6 9.46754824419245 1102 7 6.592216565004389 1102 8 7.717681532187729 1102 9 6.491773396864286 1102 10-11 7.390717928946888 1102 12-13 8.908296139995137 1102 14-15 6.036056483876052 1102 16-17 7.165062143348379 1102 18-19 7.257130865841706 1102 20-21 6.885505763782252 1102 22-23 6.401866612821479 1102 24-25 6.845116584365023 1102 26-27 8.321969722463447 1102 28-29 7.473840724349763 1102 30-31 4.078752212934969 1102 32-33 4.455853278594347 1102 34-35 5.043612313197514 1102 36-37 5.066785347245219 1102 38-39 4.511389559575424 1102 40-41 6.191074008455626 1102 42-43 6.985223764542791 1102 44-45 6.298047287799211 1102 46-47 5.517776446726131 1102 48-49 3.7190592301528262 1102 50-51 5.015279213615791 1102 52-53 5.66998329367798 1102 54-55 5.337947570418155 1102 56-57 6.302691749830224 1102 58-59 5.978452049771047 1102 60-61 5.954668698159988 1102 62-63 4.320261444142314 1102 64-65 5.979430799910151 1102 66-67 3.637338815149384 1102 68-69 5.9530623392028446 1102 70-71 5.175088864056605 1102 72-73 4.98537545389711 1102 74-75 4.035836778928481 1102 76-77 3.3158834243568194 1102 78-79 4.291004764197066 1102 80-81 4.266251782931459 1102 82-83 3.765495515052816 1102 84-85 2.921756542959603 1102 86-87 3.133307508314161 1102 88-89 3.3082273394744846 1102 90-91 3.2201166072510325 1102 92-93 2.9750789149499433 1102 94-95 2.948769596536952 1102 96-97 3.172850133946021 1102 98-99 2.858887535612208 1102 100 2.9271113897128025 1103 1 6.568829404879889 1103 2 10.706472984444108 1103 3 7.854157781776731 1103 4 11.92041699857647 1103 5 10.175898645289337 1103 6 9.137106843447178 1103 7 8.055707906815325 1103 8 7.8907945040132965 1103 9 7.047337295837391 1103 10-11 7.408028148763844 1103 12-13 8.626131789389298 1103 14-15 8.314887944456533 1103 16-17 7.847886009530036 1103 18-19 7.044003683076671 1103 20-21 8.190717576700795 1103 22-23 6.730603420879021 1103 24-25 5.2221976019601115 1103 26-27 5.779312744543784 1103 28-29 6.882661446735305 1103 30-31 5.837083179267207 1103 32-33 7.87133187674031 1103 34-35 7.212134611268134 1103 36-37 4.185330428349868 1103 38-39 6.27796026130018 1103 40-41 6.7885879927558115 1103 42-43 7.433895211698971 1103 44-45 5.481014947097659 1103 46-47 6.161720516509323 1103 48-49 5.425894029375595 1103 50-51 6.272177540864941 1103 52-53 5.854128895253076 1103 54-55 4.918877230274081 1103 56-57 5.913877002763318 1103 58-59 5.373638615790831 1103 60-61 4.793019721899579 1103 62-63 2.3910254557894497 1103 64-65 4.800171958142588 1103 66-67 3.869961523910149 1103 68-69 4.900527935440923 1103 70-71 3.5723378678520987 1103 72-73 4.2238898365789375 1103 74-75 3.028834349198366 1103 76-77 3.3588330775736743 1103 78-79 2.7733606422536816 1103 80-81 3.942746315720637 1103 82-83 3.1176154741356648 1103 84-85 3.1251228610948907 1103 86-87 2.5710154070525135 1103 88-89 2.851921579545049 1103 90-91 2.5538705959061545 1103 92-93 2.152173091022828 1103 94-95 2.669875388884801 1103 96-97 2.7912943000018444 1103 98-99 2.508941134550632 1103 100 2.76417329679121 1104 1 6.308524128857492 1104 2 9.651783980985954 1104 3 7.265622415213169 1104 4 11.98759579134753 1104 5 9.277261753590956 1104 6 8.144540474359932 1104 7 3.959687926502607 1104 8 6.427347406909323 1104 9 6.543611636560161 1104 10-11 7.0544213617347005 1104 12-13 7.337635828797588 1104 14-15 7.112246377820778 1104 16-17 7.155532478976198 1104 18-19 6.88322723527914 1104 20-21 7.5015625475794145 1104 22-23 6.345249259328206 1104 24-25 5.951574397190306 1104 26-27 8.257293752039022 1104 28-29 7.12867446081718 1104 30-31 6.385796570015952 1104 32-33 7.629222500386444 1104 34-35 8.030199211880493 1104 36-37 6.654316223107266 1104 38-39 7.270583454002711 1104 40-41 7.1145398626990755 1104 42-43 7.291374297371323 1104 44-45 6.654504042983387 1104 46-47 6.407442635007211 1104 48-49 5.340986516593862 1104 50-51 5.645055220160394 1104 52-53 5.916160474085313 1104 54-55 4.621016655231212 1104 56-57 5.930524613888888 1104 58-59 3.7050244213040813 1104 60-61 4.036021318553626 1104 62-63 3.8916840311590093 1104 64-65 2.8143864302757216 1104 66-67 3.201150519970902 1104 68-69 4.090382879972946 1104 70-71 2.4381242492441046 1104 72-73 2.4371583146750133 1104 74-75 3.0442521319841553 1104 76-77 3.616220719240463 1104 78-79 4.183625863481861 1104 80-81 2.897875730436848 1104 82-83 2.7595081603481653 1104 84-85 2.8795996734644724 1104 86-87 0.6999561798740714 1104 88-89 0.31503867264230223 1104 90-91 2.124882647774804 1104 92-93 2.2394213648178223 1104 94-95 2.4244585750621894 1104 96-97 2.709650763232805 1104 98-99 2.382299603620776 1104 100 0.8297343866684272 1105 1 5.633709779429502 1105 2 -2.7756963782113786 1105 3 0.43115224624656534 1105 4 8.259473422675544 1105 5 8.448112950038027 1105 6 7.934348048574289 1105 7 5.11005237158453 1105 8 5.693686094087095 1105 9 4.793544702683011 1105 10-11 4.935384638180597 1105 12-13 5.132367479873903 1105 14-15 5.722526164638857 1105 16-17 5.942655925823299 1105 18-19 5.960302052119655 1105 20-21 6.88328801984969 1105 22-23 2.012996768127074 1105 24-25 3.9297013947514756 1105 26-27 6.921749527184982 1105 28-29 5.7911102529578 1105 30-31 5.129082621096497 1105 32-33 6.995807036735261 1105 34-35 6.11051958518987 1105 36-37 6.082825270889053 1105 38-39 4.135162278009048 1105 40-41 5.309119931967366 1105 42-43 5.499792930031251 1105 44-45 6.058104458614348 1105 46-47 5.147931217262094 1105 48-49 4.812298742024982 1105 50-51 5.836410356999991 1105 52-53 5.324032705126395 1105 54-55 4.958777496627263 1105 56-57 4.926680276045275 1105 58-59 1.7039733353192048 1105 60-61 4.16732088414739 1105 62-63 -0.7610446382703557 1105 64-65 -0.557530432808047 1105 66-67 0.5393366327057336 1105 68-69 2.5607921019824857 1105 70-71 2.626312806981936 1105 72-73 2.483174653550698 1105 74-75 2.4018057823457077 1105 76-77 1.6661927127154996 1105 78-79 3.0980488454265114 1105 80-81 1.5481235945361256 1105 82-83 0.6692706118994742 1105 84-85 1.132035076582909 1105 86-87 -0.5429278211126451 1105 88-89 1.356320823838324 1105 90-91 1.3127155280913918 1105 92-93 1.4966931961183718 1105 94-95 0.8313702731993011 1105 96-97 1.862292817278849 1105 98-99 1.79743966542922 1105 100 1.9568630647886565 1106 1 6.206760162940441 1106 2 10.413924434205736 1106 3 6.849356065279025 1106 4 11.888993431359907 1106 5 9.953325076995291 1106 6 9.489826604816024 1106 7 6.566252108179878 1106 8 7.499649512064085 1106 9 6.904540104106555 1106 10-11 5.755649182918315 1106 12-13 7.916228434155549 1106 14-15 7.76824691046976 1106 16-17 7.651783458198565 1106 18-19 7.0882807717745955 1106 20-21 8.228289766938314 1106 22-23 6.219121387248329 1106 24-25 5.037340243169659 1106 26-27 7.658580325867074 1106 28-29 6.361684438388288 1106 30-31 5.3820286615701995 1106 32-33 7.4807247242349995 1106 34-35 6.875014406091616 1106 36-37 6.125186584430594 1106 38-39 7.164632940195361 1106 40-41 6.746286327810608 1106 42-43 6.3524140654541945 1106 44-45 4.588766909049479 1106 46-47 6.070180527209846 1106 48-49 5.928344241467386 1106 50-51 6.623183970056502 1106 52-53 5.76702924962569 1106 54-55 5.306847093129665 1106 56-57 5.051921613338692 1106 58-59 5.147217345972443 1106 60-61 4.919239047919458 1106 62-63 5.053062862178631 1106 64-65 5.213245283122461 1106 66-67 3.216570613096316 1106 68-69 3.3991989459741028 1106 70-71 2.9598971132894683 1106 72-73 2.8977323162099644 1106 74-75 2.8003201022570288 1106 76-77 3.468665248014485 1106 78-79 4.127695669959703 1106 80-81 3.693149101254807 1106 82-83 2.0892323030258915 1106 84-85 2.655529840203524 1106 86-87 3.376999332448513 1106 88-89 3.2825259931442403 1106 90-91 3.187881041932428 1106 92-93 3.0027112595833785 1106 94-95 1.540238753071069 1106 96-97 1.342247103662011 1106 98-99 1.9955571480236038 1106 100 2.876229380892344 1107 1 6.154865641546717 1107 2 10.411492561678333 1107 3 6.122227596259268 1107 4 11.396991482795201 1107 5 9.375488642152028 1107 6 8.195751890965283 1107 7 3.7023194969406745 1107 8 6.398013229382649 1107 9 5.8398587449079535 1107 10-11 4.602941729930549 1107 12-13 6.179967325040899 1107 14-15 6.204031178457019 1107 16-17 7.082829717832389 1107 18-19 6.364769486402178 1107 20-21 5.674671848565307 1107 22-23 5.913822259594085 1107 24-25 4.6173677855211785 1107 26-27 7.287311805189855 1107 28-29 6.460525888505089 1107 30-31 5.121168506421139 1107 32-33 4.9671282020735426 1107 34-35 6.738097471676014 1107 36-37 6.6012715832740625 1107 38-39 6.483913966820182 1107 40-41 7.748888576137833 1107 42-43 6.196379485736305 1107 44-45 4.2720272276881985 1107 46-47 3.559442212910536 1107 48-49 4.1459207073124595 1107 50-51 4.956938314625511 1107 52-53 3.9501736649425254 1107 54-55 5.342613316587769 1107 56-57 5.366090018740373 1107 58-59 3.220216174772503 1107 60-61 2.8089700580455848 1107 62-63 0.5505591698696328 1107 64-65 2.1216219641129896 1107 66-67 4.189351707540897 1107 68-69 5.009658895077752 1107 70-71 4.267456154330521 1107 72-73 2.483294231764514 1107 74-75 2.569827270221232 1107 76-77 3.1809650779775716 1107 78-79 3.939780176689748 1107 80-81 2.260534723148421 1107 82-83 1.6917472842767722 1107 84-85 2.2646980610210043 1107 86-87 2.313665924022235 1107 88-89 2.460144840854941 1107 90-91 1.3976661239790609 1107 92-93 2.215257521258888 1107 94-95 2.318494503771678 1107 96-97 2.3098931045557407 1107 98-99 1.902093959265855 1107 100 0.11456605530617736 1108 1 4.3745898563482974 1108 2 -3.240636937910299 1108 3 0.10267678711808514 1108 4 6.57944681047956 1108 5 6.789052786583085 1108 6 6.789883803399839 1108 7 4.1547829921735975 1108 8 5.028755499896317 1108 9 4.919849413441099 1108 10-11 3.3868228952427444 1108 12-13 2.4593242156146147 1108 14-15 3.980583011986017 1108 16-17 4.610646680238169 1108 18-19 3.9252208731961424 1108 20-21 4.660019235138396 1108 22-23 4.174677097074628 1108 24-25 2.990825373722082 1108 26-27 4.737573860064817 1108 28-29 6.148762438842432 1108 30-31 4.516012066162244 1108 32-33 4.459409132321749 1108 34-35 3.0219238364177095 1108 36-37 1.2379042584034536 1108 38-39 2.3784221979301456 1108 40-41 5.247566026826469 1108 42-43 3.0119136533889943 1108 44-45 3.7344694289452356 1108 46-47 4.621341706875757 1108 48-49 4.761452508005274 1108 50-51 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2213 50-51 -23.268633758198945 2213 52-53 -17.72957347498742 2213 54-55 -17.6003977437613 2213 56-57 -17.463355072972423 2213 58-59 -17.895204457842294 2213 60-61 -19.287780096926337 2213 62-63 -17.53885235145474 2213 64-65 -17.484379676472127 2213 66-67 -8.680043976946855 2213 68-69 -22.6324824110856 2213 70-71 -17.290793292240334 2213 72-73 -9.35922319743067 2213 74-75 -4.248815697699872 2213 76-77 -2.949972927077983 2213 78-79 -4.4707480555481744 2213 80-81 -3.213600220344169 2213 82-83 -3.2793237290337593 2213 84-85 -2.868217343129306 2213 86-87 -3.088781272309255 2213 88-89 -2.70227190952205 2213 90-91 -2.6454583790521546 2213 92-93 -3.515044576949201 2213 94-95 -3.5199899796277982 2213 96-97 -3.267566207227773 2213 98-99 -3.647240448088578 2213 100 -3.030224329729947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 745.0 3 686.0 4 3308.0 5 11434.0 6 19151.0 7 24573.0 8 23412.0 9 24071.0 10 28571.0 11 34770.0 12 43530.0 13 57074.0 14 46837.0 15 51388.0 16 57003.0 17 59276.0 18 59009.0 19 58470.0 20 57540.0 21 56502.0 22 59442.0 23 64265.0 24 70189.0 25 77559.0 26 85084.0 27 91197.0 28 98441.0 29 103115.0 30 108176.0 31 118982.0 32 135035.0 33 161250.0 34 193438.0 35 220039.0 36 232534.0 37 187969.0 38 52502.0 39 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.9114114664158 20.9170856719296 13.758272609721441 24.413230251933165 2 14.405244851402053 22.229251835417756 39.99982900940876 23.365674303771435 3 18.18578254964778 27.62126737945559 29.63057426465597 24.56237580624067 4 11.953927463268515 18.558339293100897 38.98079023220008 30.506943011430504 5 12.438862673686874 38.647031844362054 35.708442998315824 13.205662483635244 6 29.445478889746724 38.197443871051014 19.466321833860384 12.890755405341881 7 25.27952164373552 32.4745415563399 23.968313513306295 18.277623286618283 8 24.55988046938209 35.99615738198025 22.010642004618227 17.433320144019433 9 24.86833597232599 17.593969844653586 22.135512463433006 35.40218171958742 10-11 22.628744698958027 27.965244531671146 29.222186550675943 20.183824218694884 12-13 24.01827370916649 25.553537602671973 28.967152977737555 21.461035710423985 14-15 21.811656230112657 27.032971090178627 26.50302409070815 24.65234858900057 16-17 20.879042367829168 30.352861578487712 27.724319620395242 21.043776433287874 18-19 21.048779619222334 29.548893673500622 29.01627463115231 20.386052076124734 20-21 22.166681856711072 28.903414396015805 28.156377139204825 20.7735266080683 22-23 21.304534785563003 28.999535990002297 27.950288845745995 21.7456403786887 24-25 21.51317988139989 29.152648313824553 27.550459416702182 21.78371238807338 26-27 21.66903524035532 28.437495832020314 27.871526492823513 22.021942434800852 28-29 21.557375931172974 28.38392537591273 28.50731654089016 21.55138215202414 30-31 22.224849466431156 28.962903186364414 27.851616507621202 20.960630839583228 32-33 20.926790127647212 28.825252535617846 28.439433682414112 21.808523654320833 34-35 20.7490011309633 28.707964039358373 28.479000644631647 22.064034185046676 36-37 22.054543920265886 28.819347941035524 27.786478675660547 21.339629463038044 38-39 22.001988060534586 28.38485682078379 27.866307235939296 21.74684788274233 40-41 21.474306379448013 28.716330079644592 27.755434283826546 22.053929257080846 42-43 21.820859554365047 28.46685617948625 28.037987795908403 21.6742964702403 44-45 22.219060281440893 28.1707609319302 28.08145210658224 21.52872668004667 46-47 21.628428709274367 28.52288751045522 28.684854954042933 21.163828826227483 48-49 21.845020857011765 28.297162186882424 28.488338966175952 21.36947798992986 50-51 21.596991674581474 28.437866839897087 28.346720633025228 21.618420852496207 52-53 21.0776528430518 28.513096145512478 27.939952380395628 22.46929863104009 54-55 21.609651084344655 28.845216527823048 27.578928617451087 21.966203770381206 56-57 21.96213015422611 28.174278946677266 27.648453935579482 22.21513696351714 58-59 21.6750782509448 27.74903692937494 28.39264384256557 22.18324097711469 60-61 22.11890342213763 28.36073878097689 27.833233595351757 21.687124201533713 62-63 21.253452711902714 28.499592335990847 28.184353198464645 22.062601753641793 64-65 21.07472327159334 28.244801368948323 28.41674984929044 22.263725510167898 66-67 21.719634257307085 28.14700355829764 27.931452835911436 22.201909348483838 68-69 22.457456725844366 27.930251724681217 27.683458360516244 21.928833188958173 70-71 21.831995656397396 28.2512382591609 27.74832590316278 22.168440181278925 72-73 22.046649595192484 28.130446835231016 27.92665336934873 21.896250200227765 74-75 22.28023365006374 27.880263153352974 27.666545976897687 22.172957219685596 76-77 21.85194596782884 28.1437399337704 28.17539485832282 21.828919240077937 78-79 22.157238030723974 27.975560217155472 28.25155288199927 21.61564887012128 80-81 21.77654239750331 28.003647207954025 28.169487985705832 22.050322408836838 82-83 21.3567232580223 28.272701465558605 27.91363552675853 22.45693974966057 84-85 21.612789814641346 28.12879101119668 27.560554675860406 22.697864498301573 86-87 21.98865835254637 27.89097935015396 27.691614877557935 22.428747419741732 88-89 21.81468860701137 27.561996261124026 28.244895651436437 22.378419480428168 90-91 22.07616905145723 27.699999115752828 27.947227406898246 22.276604425891698 92-93 21.4096841691699 28.013183345752452 28.126986660474863 22.450145824602785 94-95 21.14702660619393 27.864914043616412 28.264269483899586 22.72378986629007 96-97 21.810485013071652 27.756462753707257 28.064644278199523 22.368407955021567 98-99 21.97638271153686 27.48875410298817 27.943692707447443 22.591170478027525 100 21.617390261470916 27.64853904724336 28.024189906880203 22.709880784405524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 389.0 1 481.0 2 460.5 3 332.5 4 354.0 5 473.5 6 672.0 7 985.5 8 1462.0 9 2038.5 10 2718.5 11 3486.5 12 4404.0 13 5390.5 14 6324.5 15 7247.0 16 8169.0 17 9063.5 18 10005.0 19 10953.5 20 11894.0 21 12927.5 22 14188.5 23 15943.0 24 18291.0 25 21232.5 26 25410.5 27 31010.5 28 38097.0 29 45799.5 30 53785.0 31 61990.0 32 69578.0 33 76931.0 34 83802.5 35 89671.0 36 94476.0 37 97831.5 38 100228.0 39 101232.0 40 100867.5 41 99906.5 42 97328.5 43 94053.5 44 90554.5 45 87342.0 46 85416.5 47 84285.5 48 83759.5 49 86730.5 50 92994.5 51 89141.5 52 74766.0 53 62977.0 54 55207.0 55 49954.0 56 45285.5 57 40344.0 58 35992.0 59 31946.0 60 27687.5 61 23449.0 62 19656.5 63 16103.0 64 13124.5 65 10655.0 66 8480.0 67 6705.5 68 5327.0 69 4135.5 70 3153.0 71 2469.0 72 1895.5 73 1449.0 74 1127.0 75 816.0 76 564.0 77 394.0 78 301.0 79 213.5 80 143.5 81 95.0 82 77.0 83 62.5 84 46.5 85 33.5 86 19.5 87 13.5 88 8.0 89 4.5 90 4.0 91 3.0 92 2.5 93 1.5 94 1.5 95 2.5 96 2.5 97 1.0 98 0.5 99 1.0 100 2.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.585553658764973 2 24.174752036648876 3 6.097449416916971 4 23.555762041618948 5 12.6020485338289 6 9.595047215679495 7 7.930469419654111 8 8.524212891933358 9 5.424508935324243 10-11 6.833722295452745 12-13 10.069436941317141 14-15 7.330225237879148 16-17 7.751114664592203 18-19 6.452899568540167 20-21 7.927660248791696 22-23 5.73429205292766 24-25 5.273930174096563 26-27 9.540790601522716 28-29 6.263784745481916 30-31 4.270479936037341 32-33 8.748694455849199 34-35 7.0616973154410765 36-37 6.959324646512667 38-39 5.925423716605081 40-41 8.36915026182913 42-43 6.4749947778233965 44-45 6.221016919852194 46-47 5.830722245031729 48-49 3.9231871844184654 50-51 6.4716633893006605 52-53 4.508755249187861 54-55 4.975833927581016 56-57 4.833250498807903 58-59 3.4107475995995133 60-61 3.948397692158091 62-63 2.9531948916308317 64-65 4.531624781208808 66-67 2.6319770080169413 68-69 5.020258443719342 70-71 2.491878615006735 72-73 1.521057976964799 74-75 0.8782080371098674 76-77 1.0736795096196095 78-79 1.615075163328075 80-81 2.2209196793223414 82-83 0.7261526604288667 84-85 1.0949823886595933 86-87 0.471382472214419 88-89 0.6303347235127601 90-91 0.21254258775057444 92-93 0.265376608970619 94-95 0.5418458413466734 96-97 0.5028595918778948 98-99 0.48224099804798637 100 0.30101346241113297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2776620.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.11611937779527 #Duplication Level Percentage of deduplicated Percentage of total 1 96.87816882696893 91.17797302421211 2 2.564881434418046 4.827933745431592 3 0.34597243238394776 0.9768474824302148 4 0.08544721263142536 0.3216784025807634 5 0.041327972007568534 0.19448141735532506 6 0.01963497647444161 0.11087806739092489 7 0.013478067353398057 0.08879523772100636 8 0.008895006817867262 0.06697308188293585 9 0.0050982213712716384 0.04318423300737257 >10 0.03241007015385124 0.5527580353140055 >50 0.0021540264526577254 0.14030258478537444 >100 0.0017559105075103715 0.361430513814054 >500 3.8792122972692607E-4 0.2320187670336913 >1k 3.103369835120263E-4 0.4733109824814543 >5k 7.758424587800658E-5 0.43143442455903624 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6582 0.23705080277459648 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5235 0.18853858288134492 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6015011056608394E-5 0.0 5 0.0 0.0 0.0 3.6015011056608394E-5 0.0 6 0.0 0.0 0.0 3.6015011056608394E-5 0.0 7 0.0 0.0 0.0 1.4406004422643358E-4 0.0 8 0.0 0.0 0.0 1.4406004422643358E-4 0.0 9 0.0 0.0 0.0 1.4406004422643358E-4 0.0 10-11 0.0 0.0 0.0 1.9808256081134618E-4 0.0 12-13 0.0 0.0 0.0 2.3409757186795458E-4 0.0 14-15 0.0 0.0 0.0 2.5210507739625875E-4 0.0 16-17 0.0 0.0 0.0 2.5210507739625875E-4 0.0 18-19 0.0 0.0 0.0 2.5210507739625875E-4 0.0 20-21 0.0 0.0 0.0 3.2413509950947556E-4 0.0 22-23 0.0 0.0 0.0 4.3218013267930076E-4 1.8007505528304197E-5 24-25 0.0 0.0 0.0 4.862026492642133E-4 3.6015011056608394E-5 26-27 0.0 0.0 0.0 5.40225165849126E-4 3.6015011056608394E-5 28-29 0.0 0.0 0.0 0.0012065028703963812 3.6015011056608394E-5 30-31 0.0 0.0 0.0 0.0026110883016041086 3.6015011056608394E-5 32-33 0.0 0.0 3.6015011056608394E-5 0.007275032233434895 3.6015011056608394E-5 34-35 3.6015011056608394E-5 0.0 3.6015011056608394E-5 0.014478034444756574 3.6015011056608394E-5 36-37 7.203002211321679E-5 1.8007505528304197E-5 3.6015011056608394E-5 0.02620092054368261 3.6015011056608394E-5 38-39 7.203002211321679E-5 3.6015011056608394E-5 3.6015011056608394E-5 0.04483868876547745 3.6015011056608394E-5 40-41 7.203002211321679E-5 3.6015011056608394E-5 3.6015011056608394E-5 0.07320050997255656 3.6015011056608394E-5 42-43 7.203002211321679E-5 3.6015011056608394E-5 3.6015011056608394E-5 0.09835699519559751 3.6015011056608394E-5 44-45 7.203002211321679E-5 3.6015011056608394E-5 3.6015011056608394E-5 0.1278172742399032 3.6015011056608394E-5 46-47 9.003752764152099E-5 3.6015011056608394E-5 3.6015011056608394E-5 0.16282386498692655 3.6015011056608394E-5 48-49 1.0804503316982519E-4 3.6015011056608394E-5 3.6015011056608394E-5 0.2004235365300257 3.6015011056608394E-5 50-51 1.0804503316982519E-4 3.6015011056608394E-5 3.6015011056608394E-5 0.24234500939991788 3.6015011056608394E-5 52-53 1.0804503316982519E-4 3.6015011056608394E-5 3.6015011056608394E-5 0.28601321030605553 3.6015011056608394E-5 54-55 1.0804503316982519E-4 3.6015011056608394E-5 3.6015011056608394E-5 0.33378712247264664 5.402251658491259E-5 56-57 1.0804503316982519E-4 3.6015011056608394E-5 3.6015011056608394E-5 0.38907016444454046 1.0804503316982519E-4 58-59 1.0804503316982519E-4 3.6015011056608394E-5 3.6015011056608394E-5 0.4521864713212467 1.0804503316982519E-4 60-61 1.8007505528304198E-4 3.6015011056608394E-5 3.6015011056608394E-5 0.521047172461482 1.0804503316982519E-4 62-63 1.8007505528304198E-4 7.203002211321679E-5 3.6015011056608394E-5 0.5932932846410384 1.0804503316982519E-4 64-65 1.8007505528304198E-4 1.0804503316982519E-4 3.6015011056608394E-5 0.6775504030079738 1.0804503316982519E-4 66-67 1.8007505528304198E-4 1.0804503316982519E-4 3.6015011056608394E-5 0.7703070639842686 1.2605253869812938E-4 68-69 1.8007505528304198E-4 1.0804503316982519E-4 3.6015011056608394E-5 0.8798107051018864 1.4406004422643358E-4 70-71 1.8007505528304198E-4 1.0804503316982519E-4 3.6015011056608394E-5 1.0237627042951503 1.4406004422643358E-4 72-73 1.8007505528304198E-4 1.0804503316982519E-4 3.6015011056608394E-5 1.1616821891364322 1.4406004422643358E-4 74-75 1.8007505528304198E-4 1.0804503316982519E-4 3.6015011056608394E-5 1.3016005070913557 2.8812008845286715E-4 76-77 1.8007505528304198E-4 1.4406004422643358E-4 3.6015011056608394E-5 1.4612370435997724 2.8812008845286715E-4 78-79 1.8007505528304198E-4 1.4406004422643358E-4 3.6015011056608394E-5 1.6376565752605687 2.8812008845286715E-4 80-81 1.8007505528304198E-4 1.4406004422643358E-4 3.6015011056608394E-5 1.8413034552801608 2.8812008845286715E-4 82-83 1.8007505528304198E-4 1.4406004422643358E-4 3.6015011056608394E-5 2.0651007339859255 2.8812008845286715E-4 84-85 1.8007505528304198E-4 1.4406004422643358E-4 3.6015011056608394E-5 2.2905547032002938 2.8812008845286715E-4 86-87 1.8007505528304198E-4 1.4406004422643358E-4 3.6015011056608394E-5 2.536357153661646 2.8812008845286715E-4 88 1.8007505528304198E-4 1.4406004422643358E-4 3.6015011056608394E-5 2.74200286679488 2.8812008845286715E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 4185 0.0 16.301104 4 GGATCGT 190 0.0026661595 15.791654 6 CTATGCG 355 2.8637987E-7 15.516991 9 GTATAGA 1120 0.0 15.435526 1 TCGACAC 290 7.684677E-5 15.426059 3 ATACCGA 270 1.0835746E-4 14.81686 6 GTGTATA 1300 0.0 14.723117 1 GTCTTAG 885 0.0 14.650667 1 TATACTG 1405 0.0 14.597444 5 CTCGTAT 325 5.9702506E-7 14.542762 94 GTACATG 21505 0.0 14.240447 1 GTACATA 1400 0.0 14.112481 1 GTATATA 1815 0.0 13.947252 1 GTATAAG 930 0.0 13.941764 1 GTCCTAA 800 5.456968E-12 13.891973 1 TACATGG 21780 0.0 13.7749815 2 GTATTAA 1545 0.0 13.587258 1 CTGTGCG 590 2.4556357E-10 13.580355 9 TCTAGAC 840 0.0 13.3141575 3 CTATAGT 895 0.0 13.120454 4 >>END_MODULE