FastQCFastQC Report
Wed 25 May 2016
SRR1295149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776620
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT181830.6548609460423105No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA137640.495710612183158No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG54380.19584963012583642No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC48710.17542911885673948No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC48360.1741685934697582No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42170.1518753016257176No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41860.15075883628296274No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG36540.13159885040084707No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32230.11607638063544885No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30100.10840518328039125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT51950.018.84243412-13
TATACGG2203.181125E-517.1009355
AGTACTT58350.016.37344412-13
GTATAGG7150.015.9951541
CATGGGG44050.015.1598544
GTACTTT66150.014.79751614-15
GTATATA21500.014.3390331
CTAGACT10650.013.6887794
ACACCGT3901.7125149E-613.2448276
TTACGCT2852.88827E-413.2007234
CCGGTAC2150.00910825813.1232643
GTCTAGG7700.012.9153421
TAGACTG12150.012.7729225
GTATTAG12100.012.73922351
GTCTTAG11050.012.5997641
TATACAG16850.012.5592935
TATACTG15050.012.49902255
CTAGTAC5807.039489E-912.1616463
TCTGTCG5453.954665E-812.0604538
TACACTG19500.012.0583525
Produced by FastQC (version 0.11.5)