FastQCFastQC Report
Wed 25 May 2016
SRR1295148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013806
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG51950.017.3927234
GTGTATA22150.017.1854951
GTACATG145550.016.9352191
TATACTG21450.016.6617745
CTGTGCG6500.016.3276569
TATACAG22800.016.1167765
TACATGG150450.016.0070632
ACATGGG147850.015.3930333
GTCTAGG11050.015.2478081
ATACCGT3704.180747E-715.0082016
GTCTAAG12300.014.7129171
GTATAGT14450.014.251221
GTATATA28000.014.040691
CTATACT16550.013.9900414
TACTGCG3707.0374645E-613.6139337
GTACTAG7854.9112714E-1113.514041
GTATAAC11600.013.4489361
GTATTAG15750.012.6787191
CCTATAC11200.012.605573
ACCGTGT8450.012.5555488
Produced by FastQC (version 0.11.5)