##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295148_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4013806 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.324427488523362 33.0 27.0 33.0 6.0 33.0 2 22.323763281035507 27.0 2.0 33.0 2.0 33.0 3 24.791552207555622 33.0 15.0 33.0 15.0 33.0 4 24.671560608559556 33.0 2.0 37.0 2.0 37.0 5 26.80672807803865 33.0 15.0 37.0 2.0 37.0 6 27.829539594090996 33.0 15.0 37.0 2.0 37.0 7 27.52712662246257 33.0 15.0 37.0 6.0 37.0 8 28.199033535751354 33.0 22.0 37.0 2.0 37.0 9 29.27344071935714 33.0 27.0 37.0 15.0 37.0 10-11 28.80721103610887 35.0 24.5 37.0 8.5 37.0 12-13 27.591809992809818 33.0 15.0 37.0 4.0 37.0 14-15 29.491350105112204 33.0 24.5 37.0 8.5 40.0 16-17 29.884862272865206 37.0 27.0 40.0 6.0 40.0 18-19 30.060119746694284 37.0 27.0 40.0 10.5 40.0 20-21 29.592047423318416 35.0 27.0 40.0 6.0 40.0 22-23 30.37511030677616 37.0 27.0 40.0 10.5 40.0 24-25 30.569480189127226 37.0 27.0 40.0 15.0 40.0 26-27 29.065299369227112 33.0 22.0 40.0 4.0 40.0 28-29 29.762769924605223 33.0 27.0 40.0 10.5 40.0 30-31 30.521706579740027 37.0 27.0 40.0 15.0 40.0 32-33 29.2701715030572 33.0 24.5 40.0 4.0 40.0 34-35 28.920889176009005 33.0 22.0 37.0 6.0 40.0 36-37 29.223749104964217 33.0 24.5 37.0 6.0 40.0 38-39 29.385079149316134 33.0 24.5 37.0 6.0 40.0 40-41 28.415645026192095 33.0 22.0 37.0 4.0 40.0 42-43 29.04724319012927 33.0 22.0 37.0 6.0 40.0 44-45 28.956485191362013 33.0 22.0 37.0 6.0 40.0 46-47 28.65478824836078 33.0 22.0 37.0 6.0 40.0 48-49 28.640548023496898 33.0 22.0 37.0 6.0 40.0 50-51 28.460842651588045 33.0 22.0 37.0 6.0 40.0 52-53 28.717021325893676 33.0 22.0 37.0 6.0 40.0 54-55 28.74848933904628 33.0 22.0 37.0 6.0 40.0 56-57 28.73550390826064 33.0 22.0 37.0 6.0 40.0 58-59 28.61737874227105 33.0 22.0 37.0 6.0 40.0 60-61 28.53480051103616 33.0 22.0 37.0 10.5 40.0 62-63 28.54722188865132 33.0 22.0 37.0 10.5 40.0 64-65 28.119037018729856 33.0 22.0 37.0 6.0 40.0 66-67 28.345568021972163 33.0 22.0 37.0 10.5 40.0 68-69 27.804648256542542 33.0 22.0 37.0 6.0 38.5 70-71 27.99494382638324 33.0 22.0 37.0 10.5 37.0 72-73 28.076414256194745 33.0 22.0 37.0 10.5 37.0 74-75 28.198585706434244 33.0 22.0 37.0 15.0 37.0 76-77 27.982751906793702 33.0 22.0 37.0 15.0 37.0 78-79 27.480064432610845 33.0 22.0 37.0 10.5 37.0 80-81 27.332488914511565 33.0 22.0 35.0 10.5 37.0 82-83 27.407058911168104 30.0 22.0 33.0 15.0 37.0 84-85 27.36756908530208 30.0 22.0 33.0 15.0 37.0 86-87 27.18831029700987 27.0 22.0 33.0 15.0 37.0 88-89 27.12575881843816 27.0 22.0 33.0 15.0 37.0 90-91 27.274035416759055 27.0 22.0 33.0 15.0 37.0 92-93 27.18220486989157 27.0 22.0 33.0 15.0 37.0 94-95 27.034222007740283 27.0 22.0 33.0 15.0 37.0 96-97 27.14377538426122 27.0 22.0 33.0 15.0 37.0 98-99 27.208579462983515 27.0 22.0 33.0 15.0 37.0 100 26.851071775765945 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.264341763723394 1101 2 11.26849662714292 1101 3 8.50028730671162 1101 4 12.931697561685485 1101 5 10.929672820867935 1101 6 10.086651701785655 1101 7 9.533658615664827 1101 8 9.20568095358436 1101 9 7.490154374211318 1101 10-11 7.923651129395232 1101 12-13 9.289403091406534 1101 14-15 8.134478594289153 1101 16-17 8.347217092417768 1101 18-19 8.208663490768007 1101 20-21 7.438162752205027 1101 22-23 7.475044926334984 1101 24-25 7.406628307360933 1101 26-27 8.701560680262073 1101 28-29 8.409651864660198 1101 30-31 5.707937284582577 1101 32-33 7.294160126974187 1101 34-35 8.80496579859117 1101 36-37 8.305820516668696 1101 38-39 8.2693454896019 1101 40-41 9.295699092067093 1101 42-43 8.76172863614449 1101 44-45 8.641292248938363 1101 46-47 7.558822269901462 1101 48-49 5.676925816002942 1101 50-51 7.032437003957121 1101 52-53 6.1476236277095175 1101 54-55 4.146865877480426 1101 56-57 6.275285022452408 1101 58-59 6.257065570739915 1101 60-61 6.774712727113872 1101 62-63 6.296720796569975 1101 64-65 6.809338042030948 1101 66-67 5.874103492842558 1101 68-69 6.95678874545456 1101 70-71 6.354066443299768 1101 72-73 5.5676174355395744 1101 74-75 4.961615795910053 1101 76-77 5.038189553316652 1101 78-79 5.22103237259077 1101 80-81 4.994923665547251 1101 82-83 4.553138690503015 1101 84-85 4.326089547521725 1101 86-87 4.135858283689739 1101 88-89 3.9836377071488336 1101 90-91 3.873191013676294 1101 92-93 3.7730438513258484 1101 94-95 3.734886175862716 1101 96-97 3.8419490964561582 1101 98-99 3.5224818871661085 1101 100 3.768185001727666 1102 1 6.728465238253069 1102 2 10.586056639839821 1102 3 8.23132055955567 1102 4 12.76718518787511 1102 5 10.809368662065978 1102 6 9.704676724316982 1102 7 6.883921722097732 1102 8 8.091432635640349 1102 9 6.700154969378563 1102 10-11 7.667699662449685 1102 12-13 9.255825314384523 1102 14-15 6.431654809138852 1102 16-17 7.610415088971468 1102 18-19 7.59610572411891 1102 20-21 7.230982789218761 1102 22-23 6.730807448937167 1102 24-25 7.122859602104754 1102 26-27 8.655973115573065 1102 28-29 7.850030589415191 1102 30-31 4.3708853675772374 1102 32-33 4.523784420567356 1102 34-35 5.284695139202544 1102 36-37 5.3741253528276545 1102 38-39 4.631034786794412 1102 40-41 6.542175673653187 1102 42-43 7.151279773764166 1102 44-45 6.625108057430552 1102 46-47 5.637076063384093 1102 48-49 3.9933611964281788 1102 50-51 5.2311400707688875 1102 52-53 5.941857264861557 1102 54-55 5.4366781943247915 1102 56-57 6.411950364163431 1102 58-59 6.039974223904789 1102 60-61 6.078188234564799 1102 62-63 4.4884006773664815 1102 64-65 6.335390871626064 1102 66-67 4.047181818268669 1102 68-69 6.396298051319512 1102 70-71 5.593508162256132 1102 72-73 5.183022709004753 1102 74-75 4.337126598195493 1102 76-77 3.377524468094375 1102 78-79 4.39450504591197 1102 80-81 4.451082782092335 1102 82-83 4.01351139273234 1102 84-85 3.194646295301183 1102 86-87 3.3680721074408773 1102 88-89 3.4795349997047076 1102 90-91 3.3698094080284413 1102 92-93 3.263101270794049 1102 94-95 3.1051132605068332 1102 96-97 3.2460536602552494 1102 98-99 3.052289407245919 1102 100 3.3163478904994115 1103 1 6.690800729908691 1103 2 11.035148954852907 1103 3 8.13835575550414 1103 4 12.021457221456561 1103 5 10.580252724470384 1103 6 9.394520968224398 1103 7 8.2544472820719 1103 8 8.372636622588448 1103 9 7.256746689578417 1103 10-11 7.710287057507635 1103 12-13 8.987448134818905 1103 14-15 8.840355227207585 1103 16-17 8.304769334238447 1103 18-19 7.509035391703289 1103 20-21 8.71023727580997 1103 22-23 7.199405389183934 1103 24-25 5.478991574165519 1103 26-27 6.308648203095583 1103 28-29 7.363921857556996 1103 30-31 6.470925687416802 1103 32-33 8.319413478043305 1103 34-35 7.555366428417734 1103 36-37 4.568037609379097 1103 38-39 6.73740991000572 1103 40-41 7.231159902605295 1103 42-43 7.767540225482328 1103 44-45 5.955738601939579 1103 46-47 6.653438100704371 1103 48-49 5.991845399888291 1103 50-51 6.770477831212112 1103 52-53 6.456770337156858 1103 54-55 5.356404622726274 1103 56-57 6.250547412190343 1103 58-59 5.809032804510476 1103 60-61 5.424311666807078 1103 62-63 3.0226837999659857 1103 64-65 5.40061583848647 1103 66-67 4.533954829043289 1103 68-69 5.732914031401766 1103 70-71 4.1667535482049445 1103 72-73 4.780320090127081 1103 74-75 3.5420400528707283 1103 76-77 3.7756310926142724 1103 78-79 3.275594318880259 1103 80-81 4.389025153187813 1103 82-83 3.7863466974466604 1103 84-85 3.571880243841342 1103 86-87 3.087561757690697 1103 88-89 3.2821523053707296 1103 90-91 2.9205414106796113 1103 92-93 2.658195931070722 1103 94-95 3.002250254490722 1103 96-97 3.216753797912027 1103 98-99 3.098806435898922 1103 100 3.189331643600937 1104 1 6.424833195588047 1104 2 9.760319579302688 1104 3 7.57398628273468 1104 4 12.275599259238124 1104 5 9.794196126393018 1104 6 8.35250697350034 1104 7 4.230424390086991 1104 8 6.844383001348707 1104 9 6.890997181757346 1104 10-11 7.223348067927041 1104 12-13 7.6635728872554125 1104 14-15 7.495061597689304 1104 16-17 7.487218554222043 1104 18-19 6.9652429816197845 1104 20-21 7.707831005998589 1104 22-23 6.63168913957583 1104 24-25 6.0732101391231055 1104 26-27 8.405630345937968 1104 28-29 7.3795072245562565 1104 30-31 6.483068023651761 1104 32-33 7.7856620037567446 1104 34-35 8.139560571069055 1104 36-37 6.749793932123374 1104 38-39 7.488922243824394 1104 40-41 7.501416522393026 1104 42-43 7.535584578710207 1104 44-45 7.0258886794483075 1104 46-47 6.941430857498553 1104 48-49 5.728194268948464 1104 50-51 6.00449213846057 1104 52-53 6.249383773729203 1104 54-55 5.109612932628114 1104 56-57 6.200397649997427 1104 58-59 4.0435816674808045 1104 60-61 4.448222197269963 1104 62-63 3.9317415001675258 1104 64-65 2.872848265034765 1104 66-67 3.5530428932745153 1104 68-69 4.549767570397343 1104 70-71 2.8822480646578903 1104 72-73 2.81721713159601 1104 74-75 3.4864518406233636 1104 76-77 4.111736499129702 1104 78-79 4.499217681235464 1104 80-81 3.227397721692025 1104 82-83 3.2733318592884117 1104 84-85 3.227212581722373 1104 86-87 1.0884039243529635 1104 88-89 0.5443689843580692 1104 90-91 2.4335329989253545 1104 92-93 2.8429171339284807 1104 94-95 2.7333370951143046 1104 96-97 3.09119608841052 1104 98-99 2.762322913884539 1104 100 0.9191818594754864 1105 1 5.8253613572248 1105 2 -2.8878352658777224 1105 3 0.43151707034036235 1105 4 8.429900347720181 1105 5 8.844068450138444 1105 6 8.391372130492677 1105 7 5.3863670818967115 1105 8 6.261401759991777 1105 9 5.224737073026297 1105 10-11 5.117366459814363 1105 12-13 5.29108328134182 1105 14-15 6.015175757359959 1105 16-17 6.328456382516389 1105 18-19 6.334913849600245 1105 20-21 7.225769674165168 1105 22-23 2.15006991865177 1105 24-25 4.096570308379945 1105 26-27 7.1546344657819 1105 28-29 6.142895855224026 1105 30-31 5.455549986641721 1105 32-33 7.218185078835621 1105 34-35 6.327471727113121 1105 36-37 6.502580797453859 1105 38-39 4.285127953396433 1105 40-41 5.782037189974982 1105 42-43 5.894113369898875 1105 44-45 6.483554627240153 1105 46-47 5.78781169644029 1105 48-49 5.437343276747022 1105 50-51 6.239625849982005 1105 52-53 5.8983167400457965 1105 54-55 5.506074671662038 1105 56-57 5.283050230092563 1105 58-59 1.9313911449407009 1105 60-61 4.599869334226955 1105 62-63 -0.6634102279069758 1105 64-65 -0.26870041882707696 1105 66-67 1.0342905604197625 1105 68-69 3.2031620368499745 1105 70-71 3.349435260872699 1105 72-73 2.9689513043243565 1105 74-75 2.843937629950897 1105 76-77 1.8411726852610677 1105 78-79 3.3706685533406784 1105 80-81 1.8195535169805517 1105 82-83 1.1941906429473583 1105 84-85 1.498162774493501 1105 86-87 -0.1047542019283405 1105 88-89 1.7826451415893452 1105 90-91 1.5997739579494628 1105 92-93 1.914613601378509 1105 94-95 1.2063741028203836 1105 96-97 2.2676386828252504 1105 98-99 2.107026591813998 1105 100 2.3248160254809456 1106 1 6.280479684480998 1106 2 10.513219561188137 1106 3 7.080092630181671 1106 4 11.981002737366818 1106 5 10.279316495131788 1106 6 9.592660940475355 1106 7 6.862305215944708 1106 8 7.719852245057151 1106 9 7.1407027221875445 1106 10-11 5.969987985082106 1106 12-13 8.157161256064569 1106 14-15 8.183803351139925 1106 16-17 8.048535741726955 1106 18-19 7.401885552904357 1106 20-21 8.49048854095766 1106 22-23 6.419808251505383 1106 24-25 5.221816597824386 1106 26-27 7.969969236493345 1106 28-29 6.516928997654887 1106 30-31 5.701589286785371 1106 32-33 7.65825171591246 1106 34-35 7.116952696719935 1106 36-37 6.300463635331667 1106 38-39 7.353125477003079 1106 40-41 7.047668918704016 1106 42-43 6.5392536234163785 1106 44-45 4.8422462329254685 1106 46-47 6.367289412768791 1106 48-49 6.123885206594046 1106 50-51 6.907305466702546 1106 52-53 5.962119771549627 1106 54-55 5.655579010269147 1106 56-57 5.241295267855243 1106 58-59 5.3814950906784595 1106 60-61 5.21879283595592 1106 62-63 5.136740364364876 1106 64-65 5.507002027324301 1106 66-67 3.2663454753045187 1106 68-69 3.710817513598805 1106 70-71 3.2528383521972764 1106 72-73 3.0812159273033046 1106 74-75 3.092458995199241 1106 76-77 3.782559914857348 1106 78-79 4.366353628041452 1106 80-81 3.975573151273718 1106 82-83 2.4554455821384416 1106 84-85 2.9540313274039143 1106 86-87 3.534998605568216 1106 88-89 3.5555211554029427 1106 90-91 3.3966559837373786 1106 92-93 3.22412387646553 1106 94-95 1.7716050341825955 1106 96-97 1.7746033526041032 1106 98-99 2.2527261381180423 1106 100 3.1349614539022994 1107 1 6.149606603124557 1107 2 10.438735155586627 1107 3 6.348442368990387 1107 4 11.580726436928519 1107 5 9.639892070681057 1107 6 8.300877780043294 1107 7 3.995804990840213 1107 8 6.667276659873053 1107 9 5.995094164888382 1107 10-11 4.759928102469281 1107 12-13 6.437210899488537 1107 14-15 6.456706036365009 1107 16-17 7.254392159219581 1107 18-19 6.475299507806373 1107 20-21 5.716117037960355 1107 22-23 5.9340973504918075 1107 24-25 4.7490892225778225 1107 26-27 7.352901542229038 1107 28-29 6.624879357734493 1107 30-31 5.250459680464225 1107 32-33 5.187757648773509 1107 34-35 6.931813830458282 1107 36-37 6.701125219191411 1107 38-39 6.67150819318223 1107 40-41 8.014373544382398 1107 42-43 6.399338206211041 1107 44-45 4.327100072665083 1107 46-47 3.6843354067846903 1107 48-49 4.369730244047226 1107 50-51 5.125401229395109 1107 52-53 4.260059857404656 1107 54-55 5.544148070488543 1107 56-57 5.46257227382533 1107 58-59 3.115305084967641 1107 60-61 3.023095134649438 1107 62-63 0.694082723337349 1107 64-65 2.3149029322969206 1107 66-67 4.317152139886559 1107 68-69 5.242791603370826 1107 70-71 4.533571082653477 1107 72-73 2.728860552561258 1107 74-75 2.789562285753963 1107 76-77 3.243474176321911 1107 78-79 4.063029701798648 1107 80-81 2.2689270067794034 1107 82-83 1.9084081587352806 1107 84-85 2.5459353282202457 1107 86-87 2.478875818073867 1107 88-89 2.600496234058131 1107 90-91 1.5165760234678807 1107 92-93 2.420163723464544 1107 94-95 2.418932695670982 1107 96-97 2.3991019586204345 1107 98-99 1.9238665376303459 1107 100 0.32293147996887583 1108 1 4.5256264514793045 1108 2 -3.3059791196174615 1108 3 0.026666532715541535 1108 4 6.904396911129432 1108 5 7.342948714300736 1108 6 7.192847398933846 1108 7 4.506501224060642 1108 8 5.424724041543865 1108 9 5.149751956845503 1108 10-11 3.5118111171384925 1108 12-13 2.7443053321246325 1108 14-15 4.230897980018799 1108 16-17 4.919885986160974 1108 18-19 4.197985210477096 1108 20-21 5.073124229616926 1108 22-23 4.348399393614315 1108 24-25 3.1818966468985224 1108 26-27 4.904265157985062 1108 28-29 6.318452459025401 1108 30-31 4.703784869047361 1108 32-33 4.727315974962135 1108 34-35 3.115931361873745 1108 36-37 1.4509688867227624 1108 38-39 2.662381187030949 1108 40-41 5.739085034845065 1108 42-43 3.296574148671766 1108 44-45 3.9573637408353406 1108 46-47 4.910066133851743 1108 48-49 5.094204200583963 1108 50-51 2.5023915491111772 1108 52-53 0.5769653070435901 1108 54-55 2.6868975433614075 1108 56-57 2.8728237564661896 1108 58-59 3.4121103874422936 1108 60-61 4.6993992022219615 1108 62-63 3.96863575432241 1108 64-65 4.39192335819779 1108 66-67 3.896243502100532 1108 68-69 4.060337313626711 1108 70-71 2.5927820908686243 1108 72-73 1.8552255827378943 1108 74-75 1.5961381826499057 1108 76-77 2.5494771118631583 1108 78-79 3.1439994939153877 1108 80-81 3.2831233461620464 1108 82-83 2.381391326699717 1108 84-85 0.12400863699536302 1108 86-87 0.3155235274707735 1108 88-89 0.4218315428476629 1108 90-91 0.11373652164726167 1108 92-93 -0.03462175356574804 1108 94-95 1.0730920791470844 1108 96-97 1.7003384916420998 1108 98-99 -0.7008040650515888 1108 100 0.739415697132241 1109 1 3.1436945447407645 1109 2 7.735626790047558 1109 3 3.8955079996323683 1109 4 -3.7842090275291724 1109 5 0.032185856057665774 1109 6 4.093169054134815 1109 7 1.806186822768872 1109 8 3.602361846250769 1109 9 2.276262351928896 1109 10-11 1.3004413530856702 1109 12-13 3.992718091629648 1109 14-15 1.9143769248307727 1109 16-17 2.1842497805662227 1109 18-19 2.597726449590308 1109 20-21 3.45676698544759 1109 22-23 3.4612141283874713 1109 24-25 3.8597136289503915 1109 26-27 5.568551814838006 1109 28-29 4.87247945486741 1109 30-31 4.103316256040003 1109 32-33 5.82683172002405 1109 34-35 1.0083344808452424 1109 36-37 2.80406542952948 1109 38-39 2.677163741732091 1109 40-41 4.141874459207681 1109 42-43 0.9744065714631063 1109 44-45 2.7357923814957488 1109 46-47 3.47467993043427 1109 48-49 2.1583807713722507 1109 50-51 3.9967860439232545 1109 52-53 2.9579143118414244 1109 54-55 3.552083675309131 1109 56-57 4.100352809033993 1109 58-59 2.399882341514246 1109 60-61 0.6241941469968886 1109 62-63 1.4679608403578506 1109 64-65 2.9136159435340225 1109 66-67 2.462238945146268 1109 68-69 1.428691387848751 1109 70-71 0.158679942377578 1109 72-73 0.3605878256554398 1109 74-75 0.9084125521744006 1109 76-77 -0.26411663129840335 1109 78-79 1.2594690919330631 1109 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52-53 -18.43609174816801 2213 54-55 -18.404531169540906 2213 56-57 -18.24580817917745 2213 58-59 -18.768602991252166 2213 60-61 -20.16927678585079 2213 62-63 -18.313322132183337 2213 64-65 -18.33754360992188 2213 66-67 -9.077221675788703 2213 68-69 -23.42138052010906 2213 70-71 -18.014401189906362 2213 72-73 -9.879688956491592 2213 74-75 -4.577758027691996 2213 76-77 -3.3458755735170307 2213 78-79 -4.864770330541177 2213 80-81 -3.5566471209010864 2213 82-83 -3.5379641865763922 2213 84-85 -3.227544284917794 2213 86-87 -3.4339095055210755 2213 88-89 -2.973608839207156 2213 90-91 -2.992348305685116 2213 92-93 -3.935658709440535 2213 94-95 -3.9956723497503788 2213 96-97 -3.620411938818897 2213 98-99 -3.9375573457644037 2213 100 -3.627497393108296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1079.0 3 1070.0 4 6979.0 5 26019.0 6 32085.0 7 38549.0 8 34687.0 9 34276.0 10 39458.0 11 47273.0 12 57104.0 13 76028.0 14 54404.0 15 60076.0 16 70710.0 17 75801.0 18 75853.0 19 75520.0 20 73487.0 21 70591.0 22 74630.0 23 82405.0 24 92260.0 25 103335.0 26 115027.0 27 125384.0 28 136081.0 29 143753.0 30 151851.0 31 165660.0 32 189280.0 33 231323.0 34 284843.0 35 339910.0 36 379002.0 37 338343.0 38 109431.0 39 239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.82651067657456 21.32979534621556 14.022684259476762 23.821009717733123 2 14.96298197853714 21.22496487034452 40.80701773249582 23.005035418622512 3 17.670780962505184 26.877982444766168 29.763365478278853 25.687871114449795 4 12.259156095618518 17.833743649751984 39.59898951976736 30.308110734862137 5 13.000480942647588 37.81157222001276 36.272382330907035 12.91556450643261 6 29.852873001089176 39.010183649254294 18.59800870614021 12.53893464351632 7 26.37358878520529 32.025726886616 24.26806465762931 17.3326196705494 8 23.637048424758344 36.56876505528354 21.984245779385958 17.809940740572156 9 24.31487024595301 17.43242259116857 22.387762576592866 35.86494458628555 10-11 22.965331845824096 27.653685925582327 29.454599895969753 19.926382332623824 12-13 23.7906964755018 25.221156457052135 29.465450005892823 21.52269706155324 14-15 21.281167098657594 26.779019649742082 26.746586347346803 25.193226904253518 16-17 20.903316011044158 30.38828874957958 27.55656107358967 21.151834165786596 18-19 20.90250994584013 29.47746083486435 29.03166510768433 20.588364111611188 20-21 22.1866634646918 28.806190351023787 28.133228594474353 20.873917589810063 22-23 21.292629308380064 28.8115307219443 28.52810614806745 21.367733821608187 24-25 21.511861010126683 28.71273145068794 28.094183892481528 21.68122364670385 26-27 21.49842746371814 28.563201882994026 28.210140502622338 21.728230150665496 28-29 21.296728454026255 28.48996476011216 28.671363527051952 21.54194325880964 30-31 21.896660109750883 28.8688050433356 28.00841603224819 21.226118814665323 32-33 21.028285879878382 28.713143298512833 28.584438014551793 21.674132807056996 34-35 21.117968218783517 28.23741455802246 28.48213675704927 22.16248046614475 36-37 21.79326132353922 28.79465387199508 27.956237212603863 21.455847591861836 38-39 21.477199720773076 28.499673958988076 28.279211951254528 21.743914368984317 40-41 21.636762197469963 28.541530590253032 28.001931700168647 21.819775512108357 42-43 21.36919618188612 28.60942074711268 28.428282496156555 21.59310057484464 44-45 21.810420712878482 28.196780514124615 28.280911266910792 21.71188750608611 46-47 21.62548258210917 28.57429049664299 28.426981588994373 21.373245332253468 48-49 21.501549459263952 28.3544068400251 28.506020806297833 21.638022894413115 50-51 21.70035049513803 28.43871440366057 28.230572884174805 21.6303622170266 52-53 21.188753959734438 28.43795412361998 28.38307140223644 21.99022051440915 54-55 21.60570769202166 28.469089202598514 28.093133440692753 21.83206966468707 56-57 21.734195387337067 28.400407730857562 28.039958994919445 21.825437886885926 58-59 21.44227770217088 27.968949225780033 28.4775359036613 22.111237168387785 60-61 21.820209530335188 28.39763504354077 28.063795646983525 21.718359779140517 62-63 21.286710582596374 28.523055604960046 28.423995661238997 21.766238151204583 64-65 21.316190466458334 28.037938805158475 28.465069371543066 22.18080135684012 66-67 21.501209537186302 28.266445318479434 28.118444337271615 22.11390080706265 68-69 21.957561893050496 28.16069308704715 28.07436754838786 21.807377471514496 70-71 21.8945122468773 28.231040283775044 27.95524204768698 21.919205421660678 72-73 21.623079452464 28.35116994117431 28.286523536054215 21.739227070307475 74-75 21.84668993492747 27.960515844231075 28.03949573160075 22.153298489240704 76-77 21.77805459867024 28.345498399448594 28.07536234153102 21.80108466035015 78-79 21.807672203670535 28.102266868419434 28.298204736303944 21.791856191606087 80-81 21.835362069216217 28.050877874581037 28.112190195487706 22.00156986071504 82-83 21.35572029504003 28.30174921698232 28.4291426770232 21.91338781095445 84-85 21.562321788371126 27.950261304332376 28.04911441349411 22.438302493802393 86-87 21.772268454389295 28.169039655433842 28.061622540590687 21.997069349586177 88-89 21.582196871289298 27.773764698965486 28.424059344097373 22.21997908564784 90-91 21.903481962731323 27.847836780668082 28.060881392995164 22.187799863605434 92-93 21.408961144461795 28.131077259406073 28.338477669476188 22.121483926655948 94-95 21.37053031009204 27.739681215010688 28.293325146813498 22.596463328083775 96-97 21.627315001481747 27.919739634014277 28.238437392733285 22.214507971770693 98-99 21.57118968754446 27.71328401934086 28.288530439016768 22.42699585409791 100 21.68069242227885 27.567721746669417 28.090068881988483 22.66151694906325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 497.0 1 546.0 2 497.5 3 432.0 4 543.0 5 799.5 6 1222.0 7 1888.0 8 2813.5 9 4078.0 10 5623.0 11 7112.5 12 8458.5 13 9826.0 14 11210.0 15 12517.0 16 13746.5 17 14948.0 18 16209.0 19 17454.0 20 18812.5 21 20534.0 22 22611.0 23 24924.0 24 28083.0 25 32493.0 26 38276.0 27 46180.5 28 55867.5 29 66655.5 30 78307.5 31 90457.5 32 102231.0 33 113186.5 34 123236.0 35 132258.0 36 140321.5 37 146712.5 38 150803.5 39 153234.0 40 153623.0 41 151227.5 42 147476.5 43 142051.0 44 135516.5 45 129178.5 46 123695.5 47 119119.0 48 114361.5 49 111169.5 50 110098.5 51 104395.5 52 92903.0 53 82980.0 54 75620.0 55 69489.5 56 64147.0 57 58349.0 58 52382.0 59 46922.5 60 40948.0 61 34772.0 62 29123.5 63 23599.0 64 18916.0 65 15213.0 66 12063.5 67 9479.5 68 7370.5 69 5618.5 70 4266.5 71 3225.5 72 2464.0 73 1858.5 74 1403.0 75 1040.0 76 733.0 77 525.5 78 357.0 79 240.0 80 169.0 81 108.0 82 72.5 83 51.5 84 33.5 85 26.0 86 19.5 87 12.5 88 7.0 89 7.5 90 5.5 91 2.5 92 2.0 93 1.5 94 2.0 95 1.0 96 0.0 97 0.5 98 1.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.6811619694624 2 25.852968479293718 3 7.004075433640788 4 25.609085242286252 5 13.800716825875492 6 10.950878044429652 7 8.95456830748671 8 10.235547009496722 9 6.262883657057665 10-11 7.725223889744546 12-13 11.320714055437657 14-15 8.358351151002315 16-17 8.962740102536097 18-19 7.563556883416886 20-21 8.91246363177493 22-23 6.536613877202835 24-25 5.889883566868952 26-27 10.790892733729532 28-29 7.2791510102879915 30-31 4.844005913589246 32-33 9.813827574127899 34-35 8.105473956638662 36-37 8.112101083111641 38-39 6.813458348510118 40-41 9.485685157678274 42-43 7.255831497586082 44-45 7.280321968724945 46-47 6.852947053245723 48-49 4.4777076919014025 50-51 7.767129253381952 52-53 5.260642891061501 54-55 5.527683699710449 56-57 5.496466446061419 58-59 4.265191192598746 60-61 4.844641220826318 62-63 3.4851958465356816 64-65 4.915322265201656 66-67 2.7518395258764374 68-69 6.073400159350004 70-71 2.990117110792101 72-73 1.9185157429133344 74-75 0.9967970549647891 76-77 1.3394892528438096 78-79 2.09885579920903 80-81 2.334766553241487 82-83 1.0820029667602271 84-85 1.5064380291424149 86-87 0.510600163535557 88-89 0.8411966099009269 90-91 0.37652542250422666 92-93 0.46428502025259816 94-95 0.7073710089625657 96-97 0.6715944916122005 98-99 0.5339072192328179 100 0.686829408297262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4013806.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.07761437378312 #Duplication Level Percentage of deduplicated Percentage of total 1 96.38549752108494 90.67717667013856 2 2.8237139316639492 5.312965407299199 3 0.4478803734883984 1.2640655118788247 4 0.13574047472004536 0.5108056014250674 5 0.07404844807022204 0.34831506712637267 6 0.03329939754248425 0.18796367285286805 7 0.02484079298934323 0.16358737805132853 8 0.01668479335700971 0.1255732444277614 9 0.010225734516510366 0.08658114376796511 >10 0.04548080473814445 0.7006629810784752 >50 0.0013747902981135459 0.08686367253959616 >100 9.199533676528599E-4 0.19686271369796665 >500 1.598095564553468E-4 0.10719599370539677 >1k 1.3317460673351552E-4 0.2313809420105532 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.491400929691171E-5 0.0 4 0.0 2.491400929691171E-5 0.0 4.982801859382342E-5 0.0 5 0.0 2.491400929691171E-5 0.0 4.982801859382342E-5 0.0 6 0.0 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 0.0 7 0.0 2.491400929691171E-5 2.491400929691171E-5 9.965603718764684E-5 0.0 8 0.0 2.491400929691171E-5 2.491400929691171E-5 1.2457004648455853E-4 0.0 9 0.0 2.491400929691171E-5 2.491400929691171E-5 1.4948405578147026E-4 0.0 10-11 0.0 2.491400929691171E-5 2.491400929691171E-5 1.8685506972683781E-4 0.0 12-13 0.0 2.491400929691171E-5 2.491400929691171E-5 1.9931207437529368E-4 0.0 14-15 0.0 2.491400929691171E-5 2.491400929691171E-5 1.9931207437529368E-4 0.0 16-17 0.0 2.491400929691171E-5 2.491400929691171E-5 2.117690790237495E-4 0.0 18-19 0.0 2.491400929691171E-5 3.7371013945367564E-5 2.2422608367220537E-4 0.0 20-21 0.0 2.491400929691171E-5 4.982801859382342E-5 2.2422608367220537E-4 0.0 22-23 0.0 2.491400929691171E-5 4.982801859382342E-5 2.615970976175729E-4 0.0 24-25 0.0 2.491400929691171E-5 4.982801859382342E-5 3.612531348052198E-4 2.491400929691171E-5 26-27 0.0 2.491400929691171E-5 4.982801859382342E-5 4.484521673444108E-4 2.491400929691171E-5 28-29 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 6.726782510166163E-4 2.491400929691171E-5 30-31 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 0.0018062656740260989 2.491400929691171E-5 32-33 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 0.006041647254501089 2.491400929691171E-5 34-35 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 0.01217049354154137 2.491400929691171E-5 36-37 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 0.021675188088313188 2.491400929691171E-5 38-39 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 0.03745821297790675 3.7371013945367564E-5 40-41 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 0.05736450640613921 4.982801859382342E-5 42-43 2.491400929691171E-5 2.491400929691171E-5 4.982801859382342E-5 0.0790646085037493 4.982801859382342E-5 44-45 4.982801859382342E-5 2.491400929691171E-5 4.982801859382342E-5 0.10518694725156123 4.982801859382342E-5 46-47 4.982801859382342E-5 3.7371013945367564E-5 4.982801859382342E-5 0.13710179316090515 4.982801859382342E-5 48-49 4.982801859382342E-5 4.982801859382342E-5 4.982801859382342E-5 0.16986371538634404 4.982801859382342E-5 50-51 4.982801859382342E-5 7.474202789073513E-5 4.982801859382342E-5 0.20540354964838858 4.982801859382342E-5 52-53 4.982801859382342E-5 7.474202789073513E-5 6.228502324227928E-5 0.24373375295168725 4.982801859382342E-5 54-55 4.982801859382342E-5 7.474202789073513E-5 7.474202789073513E-5 0.28526540644963905 4.982801859382342E-5 56-57 4.982801859382342E-5 7.474202789073513E-5 7.474202789073513E-5 0.33785888007541964 4.982801859382342E-5 58-59 4.982801859382342E-5 8.719903253919098E-5 7.474202789073513E-5 0.39380328795163494 4.982801859382342E-5 60-61 4.982801859382342E-5 9.965603718764684E-5 7.474202789073513E-5 0.45622533824504724 4.982801859382342E-5 62-63 7.474202789073513E-5 9.965603718764684E-5 7.474202789073513E-5 0.5183359634222481 4.982801859382342E-5 64-65 7.474202789073513E-5 1.1211304183610269E-4 7.474202789073513E-5 0.5888799807464536 4.982801859382342E-5 66-67 7.474202789073513E-5 1.2457004648455853E-4 7.474202789073513E-5 0.6730893321700152 4.982801859382342E-5 68-69 7.474202789073513E-5 1.2457004648455853E-4 7.474202789073513E-5 0.7686968428469139 4.982801859382342E-5 70-71 7.474202789073513E-5 1.2457004648455853E-4 7.474202789073513E-5 0.8825663223384488 4.982801859382342E-5 72-73 7.474202789073513E-5 1.2457004648455853E-4 7.474202789073513E-5 1.00348646646101 4.982801859382342E-5 74-75 7.474202789073513E-5 1.2457004648455853E-4 7.474202789073513E-5 1.1277326308247084 4.982801859382342E-5 76-77 7.474202789073513E-5 1.2457004648455853E-4 7.474202789073513E-5 1.2691943756125732 7.474202789073513E-5 78-79 7.474202789073513E-5 1.370270511330144E-4 7.474202789073513E-5 1.4307243548891002 7.474202789073513E-5 80-81 7.474202789073513E-5 1.7439806507838195E-4 7.474202789073513E-5 1.6116000623846793 7.474202789073513E-5 82-83 7.474202789073513E-5 1.7439806507838195E-4 7.474202789073513E-5 1.805916877895942 7.474202789073513E-5 84-85 9.965603718764684E-5 1.7439806507838195E-4 7.474202789073513E-5 2.005266323285181 7.474202789073513E-5 86-87 9.965603718764684E-5 1.8685506972683781E-4 7.474202789073513E-5 2.2248210302142155 7.474202789073513E-5 88 9.965603718764684E-5 1.9931207437529368E-4 7.474202789073513E-5 2.40928435504855 7.474202789073513E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 5195 0.0 17.392723 4 GTGTATA 2215 0.0 17.185495 1 GTACATG 14555 0.0 16.935219 1 TATACTG 2145 0.0 16.661774 5 CTGTGCG 650 0.0 16.327656 9 TATACAG 2280 0.0 16.116776 5 TACATGG 15045 0.0 16.007063 2 ACATGGG 14785 0.0 15.393033 3 GTCTAGG 1105 0.0 15.247808 1 ATACCGT 370 4.180747E-7 15.008201 6 GTCTAAG 1230 0.0 14.712917 1 GTATAGT 1445 0.0 14.25122 1 GTATATA 2800 0.0 14.04069 1 CTATACT 1655 0.0 13.990041 4 TACTGCG 370 7.0374645E-6 13.613933 7 GTACTAG 785 4.9112714E-11 13.51404 1 GTATAAC 1160 0.0 13.448936 1 GTATTAG 1575 0.0 12.678719 1 CCTATAC 1120 0.0 12.60557 3 ACCGTGT 845 0.0 12.555548 8 >>END_MODULE