FastQCFastQC Report
Wed 25 May 2016
SRR1295148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013806
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT87700.2184958615339157No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA66480.16562833380586903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA146100.016.5240381
GAGTACT49350.016.07601212-13
ATACCGT3906.4446795E-915.6506846
CAACGCA156750.015.2680085
TCAACGC158750.015.1052744
ATCAACG160150.015.0022123
CATGGGG62800.014.8992924
AACGCAG159500.014.8951326
GTACATG181950.014.6721761
TATCAAC162650.014.5852752
ACATGGG177450.014.1490273
TCTATAC17850.013.9604353
TACATGG186700.013.9127632
GTATAGG13500.013.727371
AGTACTT60600.013.67259312-13
ACGCAGA173250.013.6025437
CGCAGAG172300.013.5685598
GTCCTAG17500.013.4517631
GTATATA30650.013.3999371
GTATAAG16350.013.1725561
Produced by FastQC (version 0.11.5)