FastQCFastQC Report
Wed 25 May 2016
SRR1295147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709257
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46990.17344238660267372No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36750.13564604612999062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT2551.1615812E-619.732594
TCTATAC8500.016.4438273
ATAGCGC2505.635869E-515.996318
CATGGGG40750.015.7780034
GGACCGT2305.456258E-415.202256
GTACATG166250.015.0399631
TAGTCCG2000.003774349814.9886765
TACATGG168650.014.6388792
CTATACT9200.014.5849594
CGACAGT2751.2790092E-414.5309826
GTCCATA7402.0008883E-1114.2181861
CTACGCT2400.00600165613.9772514
GTGTAAG9750.013.9651341
TATACAG13400.013.79554755
GTATAAG8551.8189894E-1213.7535411
CTATACA10700.013.5853664
ACATGGG166950.013.5628323
GTATTAT12750.013.1063161
GTCTCAC15200.013.0296711
GTCTAGG6701.4917532E-812.9324351
Produced by FastQC (version 0.11.5)