##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295147_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2709257 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.468539160367584 33.0 27.0 33.0 15.0 33.0 2 22.54165551662319 27.0 6.0 33.0 2.0 33.0 3 24.875297544677377 27.0 15.0 33.0 15.0 33.0 4 25.077303482098596 33.0 6.0 37.0 2.0 37.0 5 26.933446328642873 33.0 15.0 37.0 2.0 37.0 6 27.617999695119362 33.0 15.0 37.0 6.0 37.0 7 27.465271105694292 33.0 15.0 37.0 6.0 37.0 8 28.241867419739066 33.0 22.0 37.0 6.0 37.0 9 29.258606695488837 33.0 27.0 37.0 15.0 37.0 10-11 28.73853624813002 33.0 24.5 37.0 10.5 37.0 12-13 27.54417391927012 33.0 15.0 37.0 4.0 37.0 14-15 29.363198101915028 33.0 24.5 37.0 10.5 40.0 16-17 29.7503824849396 33.0 27.0 38.5 6.0 40.0 18-19 29.858824393551444 35.0 27.0 40.0 10.5 40.0 20-21 29.39379671253041 33.0 24.5 38.5 6.0 40.0 22-23 30.125093337398408 33.0 27.0 40.0 15.0 40.0 24-25 30.229754689200767 33.0 27.0 40.0 15.0 40.0 26-27 28.811919651771685 33.0 18.5 37.0 4.0 40.0 28-29 29.48665002987904 33.0 22.0 37.0 6.0 40.0 30-31 30.166321430561958 33.0 27.0 40.0 15.0 40.0 32-33 28.932815528390257 33.0 22.0 37.0 6.0 40.0 34-35 28.594556736404115 33.0 22.0 37.0 6.0 40.0 36-37 28.909289151970448 33.0 22.0 37.0 6.0 40.0 38-39 29.006381085293867 33.0 22.0 37.0 6.0 40.0 40-41 28.071017994970575 33.0 22.0 37.0 6.0 40.0 42-43 28.65026204601483 33.0 22.0 37.0 6.0 40.0 44-45 28.52426034148846 33.0 22.0 37.0 6.0 40.0 46-47 28.15219283368097 33.0 22.0 37.0 6.0 40.0 48-49 28.04422725492635 33.0 22.0 37.0 6.0 40.0 50-51 28.012260741598155 33.0 22.0 37.0 6.0 40.0 52-53 28.20917579985952 33.0 22.0 37.0 6.0 40.0 54-55 28.167681212967246 33.0 22.0 37.0 6.0 40.0 56-57 28.145473279205333 33.0 22.0 37.0 6.0 40.0 58-59 27.981971994535773 33.0 22.0 37.0 6.0 40.0 60-61 27.932330155463287 33.0 22.0 37.0 6.0 40.0 62-63 27.86708293085521 33.0 22.0 37.0 6.0 40.0 64-65 27.43334187195973 33.0 22.0 37.0 6.0 40.0 66-67 27.64571172096261 33.0 22.0 37.0 6.0 37.0 68-69 27.222192099162243 33.0 22.0 37.0 6.0 37.0 70-71 27.314163071277477 33.0 22.0 37.0 6.0 37.0 72-73 27.33379483747758 33.0 22.0 37.0 10.5 37.0 74-75 27.43513166155887 30.0 22.0 37.0 10.5 37.0 76-77 27.17500333117161 27.0 22.0 35.0 10.5 37.0 78-79 26.732565976575867 27.0 22.0 33.0 6.0 37.0 80-81 26.578578370379777 27.0 22.0 33.0 6.0 37.0 82-83 26.66107755742626 27.0 22.0 33.0 10.5 37.0 84-85 26.61061833558057 27.0 22.0 33.0 10.5 37.0 86-87 26.38297474178345 27.0 22.0 33.0 10.5 37.0 88-89 26.295288892858817 27.0 22.0 33.0 6.0 37.0 90-91 26.43733208034528 27.0 22.0 33.0 10.5 37.0 92-93 26.35388392463321 27.0 22.0 33.0 10.5 37.0 94-95 26.214266125362045 27.0 22.0 33.0 10.5 37.0 96-97 26.310809568822744 27.0 22.0 33.0 10.5 37.0 98-99 26.345320322139983 27.0 22.0 33.0 10.5 37.0 100 26.017273001416992 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.183416039276512 1101 2 11.155092322548121 1101 3 8.380737571668142 1101 4 12.528161760923496 1101 5 10.537024623498315 1101 6 9.789541809556646 1101 7 9.23396052189937 1101 8 8.86384635185831 1101 9 7.150931837903833 1101 10-11 7.637417428652931 1101 12-13 8.961033254442484 1101 14-15 7.6963997097625345 1101 16-17 7.925959412102191 1101 18-19 7.866243018817414 1101 20-21 6.965319772279042 1101 22-23 7.176654631358005 1101 24-25 7.054079807682182 1101 26-27 8.240828621393586 1101 28-29 7.9729487751865555 1101 30-31 5.264275767876917 1101 32-33 6.748456379696176 1101 34-35 8.417858092507608 1101 36-37 7.856064899379611 1101 38-39 7.774421597176001 1101 40-41 8.696305589428317 1101 42-43 8.290771853103085 1101 44-45 8.204434559074219 1101 46-47 7.091183320259564 1101 48-49 5.106063528247947 1101 50-51 6.468989159086796 1101 52-53 5.545861089317892 1101 54-55 3.8789129464315373 1101 56-57 5.835316559841996 1101 58-59 5.80683008645628 1101 60-61 6.317726893092839 1101 62-63 5.806325593505651 1101 64-65 6.2158003242658815 1101 66-67 5.515627444524998 1101 68-69 6.340308415313288 1101 70-71 5.856603951964267 1101 72-73 5.0319765558033644 1101 74-75 4.46919282454515 1101 76-77 4.67200122892897 1101 78-79 4.716659375386989 1101 80-81 4.561101152696484 1101 82-83 4.116171320088309 1101 84-85 3.852776553247992 1101 86-87 3.6122911696512148 1101 88-89 3.6767701437698435 1101 90-91 3.3371436592596915 1101 92-93 3.239838770824033 1101 94-95 3.2284841706809075 1101 96-97 3.3451247385804166 1101 98-99 3.0370261971550825 1101 100 3.180833560471374 1102 1 6.7313424700206035 1102 2 10.540650639016121 1102 3 8.076062753875672 1102 4 12.518295868302367 1102 5 10.53411189433314 1102 6 9.451028791437096 1102 7 6.69461560637227 1102 8 7.829683441556799 1102 9 6.297574789111756 1102 10-11 7.409367928055055 1102 12-13 8.941473186450011 1102 14-15 6.040493900772695 1102 16-17 7.208583771646719 1102 18-19 7.254992431935676 1102 20-21 6.836270558203335 1102 22-23 6.265532095792494 1102 24-25 6.838285096783739 1102 26-27 8.409723085082842 1102 28-29 7.529226642479625 1102 30-31 3.966862911930505 1102 32-33 4.411368812223952 1102 34-35 5.014584045358628 1102 36-37 5.033782293957692 1102 38-39 4.350012377253925 1102 40-41 6.074675947124145 1102 42-43 6.804063751344859 1102 44-45 6.360422426377514 1102 46-47 5.45235487487022 1102 48-49 3.6460436569522052 1102 50-51 5.006213259317956 1102 52-53 5.730313317684132 1102 54-55 5.325065502610514 1102 56-57 6.206795993948774 1102 58-59 5.843585874664452 1102 60-61 5.874235691460221 1102 62-63 4.2518697873060205 1102 64-65 6.016170561260974 1102 66-67 3.692832628155479 1102 68-69 5.9021743173677095 1102 70-71 5.154523293529266 1102 72-73 4.833336720651925 1102 74-75 4.00600605073139 1102 76-77 3.1474361862933122 1102 78-79 4.145886253667118 1102 80-81 4.270665015033547 1102 82-83 3.7856735920920705 1102 84-85 3.081446790322641 1102 86-87 3.085311061063745 1102 88-89 3.1893878454528704 1102 90-91 3.2099422347410105 1102 92-93 3.0348609112253513 1102 94-95 3.0727581910075017 1102 96-97 3.1743555777085035 1102 98-99 2.8894296448089847 1102 100 3.11107032711924 1103 1 6.615067227271872 1103 2 10.867813267821571 1103 3 7.78444639228098 1103 4 11.798845763900232 1103 5 10.191191469311857 1103 6 8.908611409490412 1103 7 8.024030036986133 1103 8 7.876941807489043 1103 9 6.989194912119125 1103 10-11 7.2134528566249045 1103 12-13 8.668191246093173 1103 14-15 8.263654558506165 1103 16-17 7.874355306192999 1103 18-19 7.1019910081033615 1103 20-21 8.181428909089927 1103 22-23 6.685001970546622 1103 24-25 5.107343704639039 1103 26-27 5.825094052114519 1103 28-29 6.864169948357702 1103 30-31 5.842632453386777 1103 32-33 7.699652653929032 1103 34-35 6.940508756555531 1103 36-37 3.8665576844106724 1103 38-39 6.160035292055177 1103 40-41 6.550987074667223 1103 42-43 7.266642855688286 1103 44-45 5.365702968131028 1103 46-47 6.008470278222102 1103 48-49 5.432836236143704 1103 50-51 6.166817495861274 1103 52-53 5.808547261039092 1103 54-55 4.532080009737012 1103 56-57 5.612571803661659 1103 58-59 5.236780217683268 1103 60-61 4.656063893982019 1103 62-63 2.6665887570275686 1103 64-65 4.805472679303282 1103 66-67 3.860341178488685 1103 68-69 4.82265197549383 1103 70-71 3.305860980315515 1103 72-73 4.145923703320069 1103 74-75 3.033519293656262 1103 76-77 3.289585991892494 1103 78-79 2.657348528074099 1103 80-81 3.76274942021659 1103 82-83 3.1043123781282382 1103 84-85 3.032059957927519 1103 86-87 2.5346657035644036 1103 88-89 2.831138000955171 1103 90-91 2.50922660304386 1103 92-93 2.0760029678557146 1103 94-95 2.495551812844244 1103 96-97 2.6560538968157417 1103 98-99 2.3240288272980436 1103 100 2.5511410462646467 1104 1 6.416299463046439 1104 2 9.687775788339021 1104 3 7.473012667398464 1104 4 12.132485795276011 1104 5 9.66216835064041 1104 6 8.104567041437303 1104 7 4.013601971735014 1104 8 6.7022276523677355 1104 9 6.716319124211495 1104 10-11 7.154046064528529 1104 12-13 7.490553134481043 1104 14-15 7.1261223565291765 1104 16-17 7.211572834046141 1104 18-19 6.841432015276041 1104 20-21 7.438932271281981 1104 22-23 6.490620924222156 1104 24-25 6.0694219755565335 1104 26-27 8.367939691547004 1104 28-29 7.265794958077731 1104 30-31 6.261859083048641 1104 32-33 7.619680966139068 1104 34-35 7.968149992043415 1104 36-37 6.540887986345126 1104 38-39 7.288399606702015 1104 40-41 7.1186743479446974 1104 42-43 7.2526472774802535 1104 44-45 6.85600170902465 1104 46-47 6.593877992901167 1104 48-49 5.476216945429208 1104 50-51 5.685099766463981 1104 52-53 6.0026413616626755 1104 54-55 4.868261476528719 1104 56-57 5.993952327212696 1104 58-59 3.8961380202641607 1104 60-61 4.248906476876272 1104 62-63 3.741813719997783 1104 64-65 2.8302233928468574 1104 66-67 3.205400922685712 1104 68-69 4.206549575841969 1104 70-71 2.5766003533996162 1104 72-73 2.4538618932076837 1104 74-75 3.1271262160478273 1104 76-77 3.741149917691459 1104 78-79 4.210976823397058 1104 80-81 3.0943751811892106 1104 82-83 2.906916367179978 1104 84-85 2.860279801371867 1104 86-87 0.6071579486233531 1104 88-89 0.270000692943249 1104 90-91 2.216193179739779 1104 92-93 2.354145253106381 1104 94-95 2.375001520611086 1104 96-97 2.783019713475916 1104 98-99 2.5973639010625007 1104 100 0.800586389974125 1105 1 5.737707492617144 1105 2 -2.826727972587637 1105 3 0.3778900197319608 1105 4 8.422060144761272 1105 5 8.749979033250476 1105 6 8.018891540564582 1105 7 5.304267675519807 1105 8 6.027950838818963 1105 9 4.971403991403012 1105 10-11 5.0629942968422945 1105 12-13 5.100926819942281 1105 14-15 5.865909393864218 1105 16-17 6.070373129083027 1105 18-19 6.114874639816918 1105 20-21 7.07422049583332 1105 22-23 1.98731375123716 1105 24-25 4.065403633773734 1105 26-27 6.964165876496448 1105 28-29 6.064239548208516 1105 30-31 5.254207008480556 1105 32-33 7.156867061431161 1105 34-35 6.30906560864419 1105 36-37 6.207001524990382 1105 38-39 4.058151433892345 1105 40-41 5.399669672706441 1105 42-43 5.7141792207777975 1105 44-45 6.15773661582924 1105 46-47 5.446647960979984 1105 48-49 5.041342736569959 1105 50-51 6.020247203342095 1105 52-53 5.645473145523319 1105 54-55 5.303009917026014 1105 56-57 5.137492546415281 1105 58-59 1.9723467404880424 1105 60-61 4.350795618193445 1105 62-63 -0.8764216196774655 1105 64-65 -0.19906463154497445 1105 66-67 0.932181545070101 1105 68-69 2.7933700861076396 1105 70-71 2.9302455711083546 1105 72-73 2.6460639020626076 1105 74-75 2.5113692243894263 1105 76-77 1.7055123692369136 1105 78-79 3.1758564271576475 1105 80-81 1.6635852844535854 1105 82-83 0.8979524836269555 1105 84-85 1.3590583727825951 1105 86-87 -0.3448433011244276 1105 88-89 1.2994800592458802 1105 90-91 1.1524424491649192 1105 92-93 1.605252221905161 1105 94-95 1.0023362997368253 1105 96-97 1.9402817047867558 1105 98-99 1.8316351918547369 1105 100 2.0046530081146017 1106 1 6.06976972548923 1106 2 10.272044879150052 1106 3 6.771244418572714 1106 4 11.791366452869774 1106 5 9.826007159789786 1106 6 9.341388685105656 1106 7 6.547949708248062 1106 8 7.305141798575814 1106 9 6.728373569741535 1106 10-11 5.54150747927547 1106 12-13 7.778150905173696 1106 14-15 7.625330042220096 1106 16-17 7.466294178601306 1106 18-19 7.02108588139842 1106 20-21 8.052481006897732 1106 22-23 6.080735763330111 1106 24-25 4.841137359450354 1106 26-27 7.554684820754591 1106 28-29 6.087552640586285 1106 30-31 5.09859521609841 1106 32-33 7.200058091454171 1106 34-35 6.647007469851317 1106 36-37 5.885370876899795 1106 38-39 6.990369758015788 1106 40-41 6.575374861718824 1106 42-43 6.122662568773816 1106 44-45 4.4372076407185155 1106 46-47 5.77125237326452 1106 48-49 5.632468622694329 1106 50-51 6.40430739706159 1106 52-53 5.513948161960585 1106 54-55 5.176196566169164 1106 56-57 4.797706341956538 1106 58-59 4.778967174890642 1106 60-61 4.691283193630973 1106 62-63 4.75249276100881 1106 64-65 4.923853823144469 1106 66-67 2.7811837297740176 1106 68-69 3.1902468270771855 1106 70-71 2.7306652321530116 1106 72-73 2.6047610957899927 1106 74-75 2.6051021283821925 1106 76-77 3.2276247707119268 1106 78-79 3.9916610069337537 1106 80-81 3.488624773317479 1106 82-83 1.8625026332293437 1106 84-85 2.6101547758005985 1106 86-87 3.213659800266054 1106 88-89 3.2447512543513426 1106 90-91 3.038708641750116 1106 92-93 2.713109985992986 1106 94-95 1.3555467820136258 1106 96-97 1.3827494952817574 1106 98-99 1.8441893882142324 1106 100 2.7232380484495273 1107 1 6.182001309249195 1107 2 10.472696633981418 1107 3 6.32399432973266 1107 4 11.448946343042813 1107 5 9.703322573663367 1107 6 8.266499120945333 1107 7 3.844122856262352 1107 8 6.397883121270656 1107 9 5.889963190659785 1107 10-11 4.857512575382955 1107 12-13 6.3411552015426835 1107 14-15 6.346286680441327 1107 16-17 7.2793697962622055 1107 18-19 6.6561892741270015 1107 20-21 5.948882539717985 1107 22-23 6.105297990178109 1107 24-25 5.012925818290878 1107 26-27 7.507657419562886 1107 28-29 6.694560999028649 1107 30-31 5.243824950418592 1107 32-33 5.215357380947399 1107 34-35 6.896412294916537 1107 36-37 6.686629450231827 1107 38-39 6.635165653783446 1107 40-41 7.893070802992522 1107 42-43 6.337289593668256 1107 44-45 4.266750607613044 1107 46-47 3.608356109246838 1107 48-49 4.145216012862033 1107 50-51 5.003078520163637 1107 52-53 4.097851127972053 1107 54-55 5.344169496124746 1107 56-57 5.411918542562002 1107 58-59 3.02855829700993 1107 60-61 2.854453419715554 1107 62-63 0.6819099631489465 1107 64-65 2.282624632902845 1107 66-67 4.251429469546377 1107 68-69 5.082229715664283 1107 70-71 4.372836162548701 1107 72-73 2.597203175281006 1107 74-75 2.6592305380420065 1107 76-77 3.25995421739686 1107 78-79 4.120560764733341 1107 80-81 2.311795255774147 1107 82-83 1.7130456791719375 1107 84-85 2.4501479676972444 1107 86-87 2.3799069899650256 1107 88-89 2.621845618509383 1107 90-91 1.4738524842977725 1107 92-93 2.3624676670789206 1107 94-95 2.385218291026881 1107 96-97 2.3622098177836044 1107 98-99 1.7993011272787172 1107 100 -0.0803607035880951 1108 1 4.4024645258652235 1108 2 -3.142321688334807 1108 3 0.09380769114835275 1108 4 6.629832645065743 1108 5 7.113537674098119 1108 6 6.81742277914697 1108 7 4.243666043562676 1108 8 5.109120002136464 1108 9 5.050512504373419 1108 10-11 3.46310390590331 1108 12-13 2.730250897399344 1108 14-15 4.025324812205465 1108 16-17 4.739850659225514 1108 18-19 4.157497950010562 1108 20-21 4.848116489013201 1108 22-23 4.2121950080877255 1108 24-25 2.96387032479797 1108 26-27 4.838260383864611 1108 28-29 6.2633638935988465 1108 30-31 4.542227955989993 1108 32-33 4.477004681696659 1108 34-35 3.092632774641025 1108 36-37 1.1795529803551403 1108 38-39 2.550821023467307 1108 40-41 5.53074262362594 1108 42-43 3.1591883548662167 1108 44-45 3.8724607663590156 1108 46-47 4.937468811059148 1108 48-49 5.058786409691383 1108 50-51 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2213 50-51 -23.414279650877855 2213 52-53 -17.78946287830203 2213 54-55 -17.6918975268939 2213 56-57 -17.55394906757948 2213 58-59 -17.969400201537184 2213 60-61 -19.315829361620356 2213 62-63 -17.555805405369483 2213 64-65 -17.55136097624642 2213 66-67 -8.582511005001066 2213 68-69 -22.688727100593127 2213 70-71 -17.356492376321143 2213 72-73 -9.276362122551411 2213 74-75 -4.201463378485091 2213 76-77 -2.8585009855782957 2213 78-79 -4.297848557613509 2213 80-81 -3.212644571595323 2213 82-83 -3.0468220130324575 2213 84-85 -2.749346785871559 2213 86-87 -2.804682900630194 2213 88-89 -2.48619412232102 2213 90-91 -2.6633328324091217 2213 92-93 -3.509496098415113 2213 94-95 -3.375700925496112 2213 96-97 -3.0198823761683578 2213 98-99 -3.631548932589908 2213 100 -3.091421292128384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 752.0 3 692.0 4 3156.0 5 10871.0 6 18012.0 7 23568.0 8 22083.0 9 22501.0 10 27917.0 11 35338.0 12 42860.0 13 55564.0 14 44644.0 15 47731.0 16 52945.0 17 55939.0 18 55394.0 19 56260.0 20 56066.0 21 54021.0 22 56998.0 23 62189.0 24 68722.0 25 75421.0 26 82649.0 27 88494.0 28 95394.0 29 100295.0 30 105534.0 31 116368.0 32 131831.0 33 158213.0 34 188258.0 35 217631.0 36 230520.0 37 189735.0 38 54644.0 39 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.48027546888469 21.08348594831847 13.914896485753253 24.521342097043583 2 15.06912069979475 21.738065749860766 39.2063046958066 23.986508854537878 3 17.609361052139622 26.645186675067894 30.48996628634477 25.255485986447717 4 12.300614664545993 18.416760582449708 38.46088614419779 30.821738608806516 5 12.946727897128104 37.86158920777296 36.058094486350626 13.13358840874831 6 29.947949106878973 37.770238418004574 18.712430417652847 13.5693820574636 7 25.52871264599888 32.22042486095154 24.388082613454092 17.86277987959549 8 24.53632317983416 35.38847695096223 22.238446486840427 17.836753382363185 9 24.70869518809979 17.261010896882308 22.299348904004578 35.73094501101332 10-11 22.83684017812122 27.589917647321805 29.301142643162727 20.27209953139425 12-13 24.026675236300772 25.40290337948921 28.81186946145291 21.758551922757103 14-15 21.788430312774942 27.121147347319745 26.440348790728542 24.650073549176774 16-17 21.011340168284292 30.167752810066368 27.554335142773112 21.266571878876228 18-19 21.074115943855453 29.393870496656447 28.888165038431126 20.64384852105697 20-21 22.36999107934345 28.756109329934908 27.990684602898803 20.883214987822836 22-23 21.57593872872427 28.73698388203152 27.927213766639476 21.759863622604733 24-25 21.806014219591013 28.82118897399565 27.517388996813658 21.855407809599683 26-27 21.798075242740772 28.269649968881357 27.810259035904476 22.122015752473395 28-29 21.653633401709723 28.186099583754775 28.385813001597548 21.774454012937948 30-31 22.314071092259027 28.788388414706194 27.691200697919484 21.206339795115294 32-33 21.272896521820865 28.504573998101872 28.21104167487874 22.01148780519852 34-35 21.2177742614465 28.234381986601587 28.184670844623433 22.363172907328476 36-37 22.224118030614033 28.600248875926216 27.483470549879772 21.692162543579975 38-39 22.119346263926932 28.20685315839824 27.748901443324357 21.924899134350476 40-41 21.787497703424343 28.350739065184154 27.61720174366879 22.244561487722716 42-43 22.00300351751056 28.133282518932667 27.92423501883754 21.93947894471923 44-45 22.424708439920852 27.926927047887528 27.78971304324209 21.858651468949525 46-47 21.994511171583397 28.30171754556489 28.219200614283984 21.484570668567724 48-49 22.033631364125174 28.026956258022413 28.16570565805226 21.773706719800156 50-51 21.90156335584814 28.06479465958652 28.08608480700353 21.9475571775618 52-53 21.529345623052222 28.026490004853642 27.77452142186657 22.669642950227566 54-55 21.930929186070987 28.444165511541712 27.502844565933305 22.122060736453996 56-57 22.214170865542794 27.918753998641403 27.487544770740683 22.379530365075112 58-59 21.877002099756737 27.448032313614746 28.121877442621557 22.55308814400696 60-61 22.260572194929686 28.024013328170415 27.698330304640873 22.017084172259025 62-63 21.703530888395154 28.079990540784205 27.925688035139235 22.290790535681406 64-65 21.4271558755353 27.781516832610453 28.161070103959045 22.630257187895207 66-67 21.883836272441208 27.773590061872007 27.673229972976777 22.66934369271001 68-69 22.6073091646253 27.61323233464532 27.489274810627464 22.29018369010192 70-71 22.15435113856974 27.816482894595485 27.563975983282717 22.465189983552058 72-73 22.22599208977289 27.755750801804613 27.77707105771513 22.241186050707363 74-75 22.425466227272228 27.529808765952524 27.481782162711234 22.56294284406402 76-77 22.120375505821176 27.80495576495758 27.836987297268106 22.237681431953142 78-79 22.34512140666291 27.622378475555838 27.933283442883976 22.099216674897278 80-81 22.0510339404209 27.55981243456565 27.948165351338528 22.440988273674918 82-83 21.655705678267275 27.71185238628064 27.87174518876218 22.760696746689906 84-85 21.884903526355277 27.611470079918774 27.514100568502077 22.989525825223872 86-87 22.237035467058153 27.43533573984747 27.59550034719315 22.732128445901232 88-89 22.030480254963052 27.119190918037134 28.032896445220945 22.817432381778865 90-91 22.204347974062795 27.19683080146674 27.828743122822907 22.770078101647563 92-93 21.71797791857535 27.453718833609862 27.934027385822397 22.89427586199239 94-95 21.442175972046286 27.25908116198944 28.08296383269558 23.215779033268696 96-97 22.0082556464255 27.117644909262275 27.92872238186139 22.945377062450834 98-99 22.078501288462878 26.945768100418512 27.8074879201969 23.168242690921716 100 21.89393168073247 27.0010496131981 27.82511629973399 23.27990240633544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 445.0 1 529.5 2 508.0 3 373.0 4 378.5 5 481.0 6 644.5 7 898.5 8 1273.5 9 1818.5 10 2524.5 11 3312.5 12 4203.5 13 5025.5 14 5870.0 15 6731.0 16 7492.0 17 8306.5 18 9246.5 19 10101.5 20 11049.0 21 12015.5 22 13001.0 23 14290.5 24 16099.5 25 18539.0 26 21998.0 27 27104.5 28 33372.0 29 40671.5 30 48627.0 31 56887.5 32 65153.5 33 72791.5 34 79540.0 35 85851.0 36 90898.0 37 94455.0 38 97207.0 39 98701.5 40 98735.0 41 97616.0 42 95625.5 43 92585.0 44 88945.5 45 86072.0 46 83867.5 47 81952.5 48 81290.5 49 83363.5 50 87107.0 51 83560.5 52 72821.5 53 63609.5 54 57426.5 55 52744.0 56 48261.5 57 43867.0 58 39609.5 59 35286.5 60 30831.5 61 26407.5 62 22220.0 63 18362.5 64 14821.5 65 11819.5 66 9472.0 67 7553.5 68 5912.0 69 4586.5 70 3649.0 71 2844.5 72 2154.0 73 1681.0 74 1274.0 75 929.0 76 656.5 77 457.5 78 325.0 79 229.5 80 162.0 81 108.5 82 72.0 83 49.0 84 37.5 85 27.5 86 21.5 87 16.0 88 8.5 89 5.0 90 6.0 91 4.0 92 1.0 93 1.5 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.5 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.47083056350874 2 24.1826079991673 3 6.097022172499693 4 23.394458333041126 5 12.415359635501542 6 9.331525211524784 7 7.862118654671741 8 8.34206573979508 9 4.902894040690861 10-11 6.704882556361394 12-13 9.780079925972323 14-15 6.969973686512574 16-17 7.6340118342409005 18-19 6.571561871022203 20-21 7.709881343851838 22-23 5.619068253768469 24-25 5.208439066504211 26-27 9.706517321907814 28-29 6.2884030566313935 30-31 4.254358298234535 32-33 8.70218292321474 34-35 6.927711176902007 36-37 6.654444373494282 38-39 5.876389726039279 40-41 8.188887211512235 42-43 6.3940224201690725 44-45 6.226799450919569 46-47 5.975845037956901 48-49 3.9126040829644437 50-51 6.467492748011725 52-53 4.408957880333981 54-55 4.871243296593864 56-57 4.721275980831645 58-59 3.6436742619840055 60-61 4.058326692521233 62-63 3.0726690011320446 64-65 4.547021563476628 66-67 2.4829870329761996 68-69 4.937811363041601 70-71 2.427418292173832 72-73 1.4924202465842111 74-75 0.7811551285094032 76-77 1.073560020330297 78-79 1.676492853944827 80-81 2.04323547009383 82-83 0.7250142751315214 84-85 1.1179079725548369 86-87 0.3864897276264304 88-89 0.6428330719455555 90-91 0.22563381768507013 92-93 0.27121827128249554 94-95 0.5358111098356486 96-97 0.5082020642559935 98-99 0.4806114739207096 100 0.3049544579934646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2709257.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.77732480818062 #Duplication Level Percentage of deduplicated Percentage of total 1 96.3949761911764 90.39662992156788 2 2.891905835699792 5.423903857381848 3 0.4294126298654687 1.208075030028873 4 0.12282928632938303 0.46074407520270294 5 0.04824906135822421 0.2262333949340014 6 0.03057813114482483 0.17205212018372293 7 0.017127706475271808 0.11243333453455204 8 0.012271544649005432 0.09206341027582987 9 0.010160585586848365 0.08575492813372779 >10 0.038403739799328554 0.6090143410946413 >50 0.002093362685839238 0.12821430846024154 >100 0.0014352829949283697 0.3381915612761871 >500 2.3856095782872605E-4 0.14940505827050812 >1k 3.180812769020316E-4 0.5972846586553149 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4699 0.17344238660267372 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3675 0.13564604612999062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.382097748570918E-5 0.0 5 0.0 0.0 0.0 1.1073146622856377E-4 0.0 6 0.0 0.0 0.0 1.1073146622856377E-4 0.0 7 0.0 0.0 0.0 1.1073146622856377E-4 0.0 8 0.0 0.0 0.0 1.1073146622856377E-4 0.0 9 0.0 0.0 0.0 1.4764195497141835E-4 0.0 10-11 3.691048874285459E-5 0.0 0.0 1.4764195497141835E-4 0.0 12-13 3.691048874285459E-5 0.0 0.0 1.4764195497141835E-4 0.0 14-15 3.691048874285459E-5 0.0 0.0 2.2146293245712753E-4 0.0 16-17 3.691048874285459E-5 0.0 0.0 2.2146293245712753E-4 0.0 18-19 5.536573311428188E-5 0.0 0.0 2.2146293245712753E-4 0.0 20-21 7.382097748570918E-5 0.0 0.0 2.2146293245712753E-4 0.0 22-23 7.382097748570918E-5 0.0 0.0 2.2146293245712753E-4 0.0 24-25 7.382097748570918E-5 0.0 0.0 3.875601317999732E-4 0.0 26-27 7.382097748570918E-5 0.0 0.0 4.98291598028537E-4 0.0 28-29 7.382097748570918E-5 0.0 0.0 0.0010150384404285012 0.0 30-31 1.1073146622856377E-4 0.0 0.0 0.003248123009371204 0.0 32-33 1.1073146622856377E-4 0.0 0.0 0.010962415156627813 0.0 34-35 1.1073146622856377E-4 0.0 0.0 0.02290295826494127 0.0 36-37 1.1073146622856377E-4 0.0 0.0 0.042041046678111385 0.0 38-39 1.1073146622856377E-4 0.0 0.0 0.07461455299368056 0.0 40-41 1.1073146622856377E-4 0.0 0.0 0.11698779407047763 0.0 42-43 1.1073146622856377E-4 0.0 0.0 0.15614982262664634 0.0 44-45 1.1073146622856377E-4 0.0 0.0 0.2033767929731288 0.0 46-47 1.1073146622856377E-4 0.0 0.0 0.25852106315495355 0.0 48-49 1.4764195497141835E-4 0.0 0.0 0.318186868207778 0.0 50-51 1.4764195497141835E-4 0.0 0.0 0.3799196606302023 0.0 52-53 1.8455244371427294E-4 0.0 0.0 0.44955129764359747 0.0 54-55 1.8455244371427294E-4 0.0 0.0 0.5267495848492778 0.0 56-57 2.2146293245712753E-4 0.0 0.0 0.6165158934719002 0.0 58-59 2.2146293245712753E-4 0.0 0.0 0.7167463256531219 0.0 60-61 2.2146293245712753E-4 0.0 0.0 0.8210000011073146 0.0 62-63 2.2146293245712753E-4 3.691048874285459E-5 0.0 0.9301074058311929 0.0 64-65 2.2146293245712753E-4 3.691048874285459E-5 0.0 1.0596632213186123 0.0 66-67 2.2146293245712753E-4 3.691048874285459E-5 0.0 1.209039969260945 0.0 68-69 2.2146293245712753E-4 3.691048874285459E-5 0.0 1.375930744111762 0.0 70-71 2.2146293245712753E-4 3.691048874285459E-5 0.0 1.570928856140263 0.0 72-73 2.2146293245712753E-4 3.691048874285459E-5 0.0 1.7730137820073917 0.0 74-75 2.2146293245712753E-4 3.691048874285459E-5 0.0 1.987463721603377 0.0 76-77 2.2146293245712753E-4 7.382097748570918E-5 0.0 2.229024415180989 0.0 78-79 2.2146293245712753E-4 7.382097748570918E-5 0.0 2.4956657858593703 0.0 80-81 2.2146293245712753E-4 7.382097748570918E-5 0.0 2.794880662853321 0.0 82-83 2.2146293245712753E-4 7.382097748570918E-5 0.0 3.128736033532441 0.0 84-85 2.2146293245712753E-4 7.382097748570918E-5 0.0 3.4628497776327603 0.0 86-87 2.2146293245712753E-4 7.382097748570918E-5 0.0 3.824074275714707 0.0 88 2.2146293245712753E-4 7.382097748570918E-5 0.0 4.133974739199714 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGGT 255 1.1615812E-6 19.73259 4 TCTATAC 850 0.0 16.443827 3 ATAGCGC 250 5.635869E-5 15.99631 8 CATGGGG 4075 0.0 15.778003 4 GGACCGT 230 5.456258E-4 15.20225 6 GTACATG 16625 0.0 15.039963 1 TAGTCCG 200 0.0037743498 14.988676 5 TACATGG 16865 0.0 14.638879 2 CTATACT 920 0.0 14.584959 4 CGACAGT 275 1.2790092E-4 14.530982 6 GTCCATA 740 2.0008883E-11 14.218186 1 CTACGCT 240 0.006001656 13.977251 4 GTGTAAG 975 0.0 13.965134 1 TATACAG 1340 0.0 13.7955475 5 GTATAAG 855 1.8189894E-12 13.753541 1 CTATACA 1070 0.0 13.585366 4 ACATGGG 16695 0.0 13.562832 3 GTATTAT 1275 0.0 13.106316 1 GTCTCAC 1520 0.0 13.029671 1 GTCTAGG 670 1.4917532E-8 12.932435 1 >>END_MODULE