FastQCFastQC Report
Wed 25 May 2016
SRR1295147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709257
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132350.4885103185116805No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA96940.3578102778732324No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG38810.14324960681101867No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC35640.13154898187953376No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35460.13088459308216238No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32960.12165697089644874No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG29690.10958724107753529No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG27380.10106091817793587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG50500.018.7157484
GAGTACT41550.017.23166512-13
TCTGTCG6750.016.693068
GTCTAGA10150.016.1476251
CTAGACT9300.015.6740514
CTGTCGC7600.015.4438269
TAGCGTG2155.1729276E-415.3095385
AATACCG2206.159245E-414.9615955
CGCGTGA950.00405924814.82607310-11
ATGGGGG32050.014.8182135
AGTACTT50800.014.55610912-13
GTATATA16750.014.529211
GTATAGG7550.014.4722631
GTACTAG5257.039489E-1014.1903361
GTACTTT53800.013.74430314-15
GTTAGAC5301.8408173E-913.3079113
GTGTATA12850.013.1412451
TTATACT15700.012.8786784
GTACATG218850.012.7087331
GTGTAAG9550.012.4815521
Produced by FastQC (version 0.11.5)