##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295147_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2709257 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28245124032161 33.0 33.0 33.0 27.0 33.0 2 29.998400668522773 33.0 33.0 33.0 27.0 33.0 3 30.80679315399019 33.0 33.0 33.0 27.0 33.0 4 35.041807772389255 37.0 37.0 37.0 33.0 37.0 5 35.38099338674773 37.0 37.0 37.0 33.0 37.0 6 35.13652414665718 37.0 37.0 37.0 33.0 37.0 7 35.01589107271846 37.0 37.0 37.0 33.0 37.0 8 35.01815257836373 37.0 37.0 37.0 33.0 37.0 9 34.95961365053223 37.0 37.0 37.0 33.0 37.0 10-11 34.94411899646287 37.0 37.0 37.0 33.0 37.0 12-13 34.86050769639056 37.0 37.0 37.0 30.0 37.0 14-15 36.27774459934956 37.0 37.0 40.0 27.0 40.0 16-17 36.27348715902552 37.0 37.0 40.0 27.0 40.0 18-19 36.304687226054966 38.5 37.0 40.0 27.0 40.0 20-21 36.29964211590115 40.0 37.0 40.0 27.0 40.0 22-23 36.258736989514105 37.0 37.0 40.0 27.0 40.0 24-25 36.22441392603211 37.0 37.0 40.0 27.0 40.0 26-27 36.192732361677024 37.0 37.0 40.0 27.0 40.0 28-29 36.12747424847477 37.0 37.0 40.0 27.0 40.0 30-31 36.01984160232861 37.0 37.0 40.0 27.0 40.0 32-33 35.97068605894531 37.0 37.0 40.0 27.0 40.0 34-35 35.875275213831685 37.0 37.0 40.0 27.0 40.0 36-37 35.84518596796096 37.0 37.0 40.0 27.0 40.0 38-39 35.76383931092546 37.0 37.0 40.0 27.0 40.0 40-41 35.687896533994376 37.0 37.0 40.0 27.0 40.0 42-43 35.58333151856764 37.0 37.0 40.0 27.0 40.0 44-45 35.456433442822146 37.0 37.0 40.0 27.0 40.0 46-47 35.331408205275466 37.0 35.0 40.0 27.0 40.0 48-49 35.19918449966171 37.0 33.0 40.0 27.0 40.0 50-51 35.09106463506415 37.0 33.0 40.0 27.0 40.0 52-53 34.99592397472813 37.0 33.0 40.0 27.0 40.0 54-55 34.86452281935601 37.0 33.0 40.0 24.5 40.0 56-57 34.72542619618589 37.0 33.0 40.0 22.0 40.0 58-59 34.59490000394942 37.0 33.0 40.0 22.0 40.0 60-61 34.42594427180589 37.0 33.0 40.0 22.0 40.0 62-63 34.23356145245726 37.0 33.0 40.0 22.0 40.0 64-65 34.05003936503624 37.0 33.0 40.0 22.0 40.0 66-67 33.82216858718091 37.0 33.0 38.5 22.0 40.0 68-69 33.60368691489955 37.0 33.0 37.0 22.0 40.0 70-71 33.35848297153058 37.0 33.0 37.0 22.0 40.0 72-73 33.13790921274726 37.0 33.0 37.0 22.0 40.0 74-75 32.896972860086734 37.0 33.0 37.0 22.0 40.0 76-77 32.65881697454321 37.0 33.0 37.0 22.0 37.0 78-79 32.42545539238249 37.0 33.0 37.0 22.0 37.0 80-81 32.23234506730074 37.0 33.0 37.0 22.0 37.0 82-83 32.03818629978625 33.0 33.0 37.0 22.0 37.0 84-85 31.857818582733202 33.0 33.0 37.0 22.0 37.0 86-87 31.880941896615937 33.0 33.0 37.0 22.0 37.0 88-89 31.898463121069724 33.0 33.0 37.0 22.0 37.0 90-91 31.91763221429344 33.0 33.0 37.0 22.0 37.0 92-93 31.885681572475406 33.0 33.0 37.0 22.0 37.0 94-95 31.928132510131007 33.0 33.0 37.0 22.0 37.0 96-97 31.896392073546366 33.0 33.0 37.0 22.0 37.0 98-99 31.817109266488927 33.0 33.0 37.0 22.0 37.0 100 31.267609532798108 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5843721966182045 1101 2 4.043923140115879 1101 3 2.2718845202784195 1101 4 1.0859913853784704 1101 5 0.39665157309961785 1101 6 0.39249183156989176 1101 7 0.46415648914285157 1101 8 0.394806942751444 1101 9 0.5400777614123555 1101 10-11 0.4793677848226565 1101 12-13 0.5754602125321782 1101 14-15 0.8785066734747744 1101 16-17 0.8539545997284606 1101 18-19 0.8533875288493888 1101 20-21 0.8512599258647171 1101 22-23 0.7605848289429886 1101 24-25 0.8222790083639069 1101 26-27 0.9037419901751207 1101 28-29 0.6955657312065213 1101 30-31 0.6050747874882205 1101 32-33 0.9029812394480459 1101 34-35 0.9904833826742774 1101 36-37 0.8944556548253644 1101 38-39 0.870777454331332 1101 40-41 0.93985206089814 1101 42-43 0.9252474802574397 1101 44-45 1.011520933961613 1101 46-47 0.9461529169444205 1101 48-49 0.8850479139584593 1101 50-51 0.9320191607741606 1101 52-53 0.8270140362651262 1101 54-55 0.8347124732902884 1101 56-57 0.9059316368561383 1101 58-59 0.9447187216837634 1101 60-61 0.9546817173093913 1101 62-63 0.7979668550875232 1101 64-65 0.8480200034805847 1101 66-67 0.8255876182573587 1101 68-69 0.7761810848537678 1101 70-71 0.8425038545420165 1101 72-73 0.7199683309740621 1101 74-75 0.8033709212967111 1101 76-77 0.7899872407772932 1101 78-79 0.8200487705386195 1101 80-81 0.8568719283929127 1101 82-83 0.8934119587749478 1101 84-85 0.8687254073395962 1101 86-87 0.8384261163929523 1101 88-89 0.7933216002635604 1101 90-91 0.7978041191327669 1101 92-93 0.8849350101825557 1101 94-95 0.8072443800354776 1101 96-97 0.6647311596840844 1101 98-99 0.7435122815837083 1101 100 0.733406427678446 1102 1 0.4870294425661754 1102 2 3.990198726937951 1102 3 2.244414931578099 1102 4 1.044004521755184 1102 5 0.4263077544489491 1102 6 0.45548269684298504 1102 7 0.4526420995583962 1102 8 0.5276194880355334 1102 9 0.559270399667831 1102 10-11 0.5805732464512445 1102 12-13 0.6281089173689907 1102 14-15 0.8024452088429825 1102 16-17 0.8362885075078665 1102 18-19 0.8843176740391883 1102 20-21 0.9055726840798997 1102 22-23 0.8477309567282418 1102 24-25 0.8512991525433122 1102 26-27 0.8028051075659022 1102 28-29 0.8061298042584184 1102 30-31 0.8225535122792991 1102 32-33 0.8702617371533066 1102 34-35 0.9854130852947236 1102 36-37 0.8576781728767742 1102 38-39 0.9256531416691587 1102 40-41 0.8374807063415588 1102 42-43 0.9593631530429789 1102 44-45 0.9972997139545257 1102 46-47 0.945210368674644 1102 48-49 0.8413850206482465 1102 50-51 0.8611201102297628 1102 52-53 0.8005786010986355 1102 54-55 0.7673900059400012 1102 56-57 0.7267433299612307 1102 58-59 0.8018981734410389 1102 60-61 0.8208690152962035 1102 62-63 0.8862335135786736 1102 64-65 0.8206646088113487 1102 66-67 0.8344347716919742 1102 68-69 0.793104972699382 1102 70-71 0.7967853976383665 1102 72-73 0.7849715286342374 1102 74-75 0.6448999944321514 1102 76-77 0.6109466843082743 1102 78-79 0.5773593195439659 1102 80-81 0.5855247337586604 1102 82-83 0.7088846334133976 1102 84-85 0.7387300933011858 1102 86-87 0.6883465186923559 1102 88-89 0.6466452631420836 1102 90-91 0.6995637954502207 1102 92-93 0.8466137777985345 1102 94-95 0.8348192267074666 1102 96-97 0.7469247216070265 1102 98-99 0.7520656247723743 1102 100 0.6945636362910257 1103 1 0.41495584456394496 1103 2 3.886135002185288 1103 3 2.2470015908564953 1103 4 1.049309604432871 1103 5 0.392947234970336 1103 6 0.4458542831316237 1103 7 0.350883272165035 1103 8 0.3219311082319294 1103 9 0.4073946473852956 1103 10-11 0.47294076674452157 1103 12-13 0.46891968299078 1103 14-15 0.7012900996519278 1103 16-17 0.633394754113418 1103 18-19 0.6774334402297839 1103 20-21 0.696205631210951 1103 22-23 0.6471132020347312 1103 24-25 0.6694908121152849 1103 26-27 0.6157698943213248 1103 28-29 0.6567881890894682 1103 30-31 0.6349484106110737 1103 32-33 0.6676221084689615 1103 34-35 0.7897267949099955 1103 36-37 0.6739063864993255 1103 38-39 0.6730165978019116 1103 40-41 0.5748700542960847 1103 42-43 0.7255778232863506 1103 44-45 0.7522042291176092 1103 46-47 0.6302218730929212 1103 48-49 0.6255827411404198 1103 50-51 0.6022176032940934 1103 52-53 0.4639826818644224 1103 54-55 0.49173803156531903 1103 56-57 0.5415203607571044 1103 58-59 0.6600604618845054 1103 60-61 0.4826654361744289 1103 62-63 0.6283422876700655 1103 64-65 0.5937308413253746 1103 66-67 0.5916647687745851 1103 68-69 0.6387343029110468 1103 70-71 0.6976447657577296 1103 72-73 0.6609668121897059 1103 74-75 0.6157417068473805 1103 76-77 0.5556300077640799 1103 78-79 0.5768553131807579 1103 80-81 0.5859727776405848 1103 82-83 0.6133566778604518 1103 84-85 0.46328574202560446 1103 86-87 0.607155221959097 1103 88-89 0.49826722327845374 1103 90-91 0.53898547595767 1103 92-93 0.6014791880310462 1103 94-95 0.6438785088647947 1103 96-97 0.5390415919098466 1103 98-99 0.5934064858239587 1103 100 0.6054038212057407 1104 1 0.31849322544093184 1104 2 3.5342455052938213 1104 3 2.1137253987831635 1104 4 0.8178608884599825 1104 5 0.35799343671216377 1104 6 0.21816188868876196 1104 7 0.23224338951736456 1104 8 0.2226602799733115 1104 9 0.24959646978473415 1104 10-11 0.35230309674947335 1104 12-13 0.39258839371250787 1104 14-15 0.5445851760958362 1104 16-17 0.4246112791643881 1104 18-19 0.44965110627124005 1104 20-21 0.45229046982104393 1104 22-23 0.41974108536912524 1104 24-25 0.47649325921960894 1104 26-27 0.36632437494873216 1104 28-29 0.3648156539006351 1104 30-31 0.41346639535226615 1104 32-33 0.5130916015239464 1104 34-35 0.6370645823182741 1104 36-37 0.5543543292031643 1104 38-39 0.43927173067817193 1104 40-41 0.3991825352073519 1104 42-43 0.467721641724701 1104 44-45 0.45166805844238667 1104 46-47 0.4709639349937973 1104 48-49 0.3163908143127756 1104 50-51 0.31311636957688904 1104 52-53 0.38756148234766385 1104 54-55 0.4756358280706081 1104 56-57 0.378896600236061 1104 58-59 0.48001030538038236 1104 60-61 0.45530633029031975 1104 62-63 0.5165127030266703 1104 64-65 0.3863554819186348 1104 66-67 0.3595723792268686 1104 68-69 0.3908063079251818 1104 70-71 0.29086915243581757 1104 72-73 0.32096057380690013 1104 74-75 0.2861178320281965 1104 76-77 0.28152951124641845 1104 78-79 0.34629756053503513 1104 80-81 0.33971454840911264 1104 82-83 0.45077952661965526 1104 84-85 0.4663565089660864 1104 86-87 0.4663197336296818 1104 88-89 0.35617600716035014 1104 90-91 0.39066747079270314 1104 92-93 0.33830413224043454 1104 94-95 0.3546345342267969 1104 96-97 0.3874012116671217 1104 98-99 0.35145818043432797 1104 100 0.2833860715017913 1105 1 0.4626627126372611 1105 2 3.7281313523557884 1105 3 2.148576079347542 1105 4 0.9594631903237456 1105 5 0.36396439183307905 1105 6 0.39633315674628733 1105 7 0.35092190174730575 1105 8 0.3236008386240883 1105 9 0.34527295088060583 1105 10-11 0.28922313567035474 1105 12-13 0.35118570760715073 1105 14-15 0.4576030392299302 1105 16-17 0.43223353554990496 1105 18-19 0.5113735244156246 1105 20-21 0.41198814666278594 1105 22-23 0.39408644105860446 1105 24-25 0.4402175810837434 1105 26-27 0.3729290215591803 1105 28-29 0.3498468527723304 1105 30-31 0.3818577110160106 1105 32-33 0.528380114116878 1105 34-35 0.6036244531580763 1105 36-37 0.42686521269284583 1105 38-39 0.4430993183901464 1105 40-41 0.34386348726709315 1105 42-43 0.4892833326640158 1105 44-45 0.5347170168413911 1105 46-47 0.4931028406973539 1105 48-49 0.457741535364633 1105 50-51 0.4223962318107155 1105 52-53 0.4458910498311752 1105 54-55 0.5240240370001032 1105 56-57 0.45085539912027883 1105 58-59 0.49361558230224034 1105 60-61 0.4705767080201255 1105 62-63 0.41064694186869133 1105 64-65 0.44416808823500276 1105 66-67 0.38907691977629355 1105 68-69 0.3804089619050828 1105 70-71 0.37114287424025605 1105 72-73 0.3677542962050566 1105 74-75 0.4586498483648569 1105 76-77 0.3878574027981756 1105 78-79 0.4265869041867205 1105 80-81 0.4988957049636866 1105 82-83 0.4182639668630088 1105 84-85 0.5471265881466678 1105 86-87 0.45422836507503916 1105 88-89 0.46768891179081606 1105 90-91 0.305665796183586 1105 92-93 0.4848784580651433 1105 94-95 0.3710261138306876 1105 96-97 0.42925665211871333 1105 98-99 0.42183253015472744 1105 100 0.3651404740190394 1106 1 0.3917895409017511 1106 2 3.7693281140896993 1106 3 2.1470135894303795 1106 4 0.94614000973327 1106 5 0.30308077338405326 1106 6 0.22454415884925538 1106 7 0.13222006184945911 1106 8 0.14920138125091142 1106 9 0.12067649731398689 1106 10-11 0.20669015538861402 1106 12-13 0.24921409656905524 1106 14-15 0.2757099828886638 1106 16-17 0.22710668720937122 1106 18-19 0.24723385823724442 1106 20-21 0.2472922306322758 1106 22-23 0.13480919302551797 1106 24-25 0.2044868811539331 1106 26-27 0.22097644691393015 1106 28-29 0.2353527368460817 1106 30-31 0.13354779137038975 1106 32-33 0.3212498915667439 1106 34-35 0.3432825294284072 1106 36-37 0.14297867676425824 1106 38-39 0.1383692587427845 1106 40-41 0.23107841638790205 1106 42-43 0.21345897798339308 1106 44-45 0.19869241795002068 1106 46-47 0.15126680032442863 1106 48-49 0.13366153806055792 1106 50-51 0.12625083248742186 1106 52-53 0.09134307274367615 1106 54-55 0.1849996467561965 1106 56-57 0.1493694849754874 1106 58-59 0.16182925165120565 1106 60-61 0.17330661996515317 1106 62-63 0.23528376262618167 1106 64-65 0.1626018412293817 1106 66-67 0.057906995412793094 1106 68-69 0.057781753271235914 1106 70-71 0.05114032171451299 1106 72-73 0.11480021370541493 1106 74-75 -2.1676704747619624E-5 1106 76-77 0.03447390803192718 1106 78-79 0.13580862044480568 1106 80-81 0.12623167829696058 1106 82-83 0.1036040084634422 1106 84-85 0.12194728396805132 1106 86-87 0.1014230604102977 1106 88-89 0.1549862960972277 1106 90-91 0.15011602444105066 1106 92-93 0.23428503167933812 1106 94-95 0.28586790383956284 1106 96-97 0.2263137189941702 1106 98-99 0.23975549119931472 1106 100 0.18466471476810753 1107 1 0.3522189356713348 1107 2 3.4695297222548653 1107 3 2.0500757404349663 1107 4 0.8905210812119435 1107 5 0.2930962198026421 1107 6 0.13441227677186873 1107 7 0.10111813898755173 1107 8 0.0700187239684098 1107 9 -0.001147533154409075 1107 10-11 0.08277448471565663 1107 12-13 0.026061132872051473 1107 14-15 0.03035035337482128 1107 16-17 0.03371709919220223 1107 18-19 0.055462405412200155 1107 20-21 0.06671996539255787 1107 22-23 0.031901577421287186 1107 24-25 0.044373464988680666 1107 26-27 0.02514256463572906 1107 28-29 0.05326196545806283 1107 30-31 -0.08729575827215541 1107 32-33 0.014672309454674348 1107 34-35 0.14340152880812695 1107 36-37 0.03506119439517619 1107 38-39 0.01938041876240959 1107 40-41 -0.05634881132534986 1107 42-43 0.0700813183899811 1107 44-45 0.2223405358584003 1107 46-47 0.049519838155688944 1107 48-49 -0.04756376387098982 1107 50-51 -0.03493521602815264 1107 52-53 -0.012007995366552393 1107 54-55 0.08304181854116877 1107 56-57 0.005145512618099701 1107 58-59 0.04852923719467839 1107 60-61 0.08507538088321809 1107 62-63 0.06280324156413997 1107 64-65 -0.028390026790269474 1107 66-67 0.005393160221586868 1107 68-69 -0.0029965211879101616 1107 70-71 0.08728045470041224 1107 72-73 0.020479781348122117 1107 74-75 0.06611790792972982 1107 76-77 0.10764022444209331 1107 78-79 0.1064618494157088 1107 80-81 0.020276937941360984 1107 82-83 -0.04229094022297275 1107 84-85 0.0651459815978157 1107 86-87 0.18607192678275197 1107 88-89 0.15938941557588038 1107 90-91 0.15860877730048983 1107 92-93 0.10585407873714914 1107 94-95 0.08645663386479185 1107 96-97 0.0714246675780359 1107 98-99 0.10566419852137088 1107 100 0.16074348936647453 1108 1 0.4140303901430471 1108 2 3.647653944001142 1108 3 2.0911190095631085 1108 4 1.0230608032819717 1108 5 0.28436142020964894 1108 6 0.09312039338006883 1108 7 0.004313693246508876 1108 8 0.04547393568900304 1108 9 0.06595739857272775 1108 10-11 0.04938389562651224 1108 12-13 0.12607792536381623 1108 14-15 0.02074881928108141 1108 16-17 -0.022368827134293667 1108 18-19 0.06253659834084857 1108 20-21 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82-83 -0.366812678029234 2211 84-85 0.16770694989535073 2211 86-87 0.14180988342040024 2211 88-89 0.15730343386660905 2211 90-91 0.013121229201434659 2211 92-93 0.09656931552138914 2211 94-95 0.25592085267333786 2211 96-97 0.0777690950355101 2211 98-99 0.18236923445151376 2211 100 0.09550358021416372 2212 1 0.6471410581390593 2212 2 3.9700439169394706 2212 3 2.2998969614218154 2212 4 1.0085187152603226 2212 5 0.5800771302224632 2212 6 0.5151300786100919 2212 7 0.4789683008522658 2212 8 0.47552663473472023 2212 9 0.4349732082864435 2212 10-11 0.4843354694763917 2212 12-13 0.4497369482950191 2212 14-15 0.5357872753414767 2212 16-17 0.5456756109799343 2212 18-19 0.6033783587738384 2212 20-21 0.5423479471784205 2212 22-23 0.5317517577379647 2212 24-25 0.38871783601253185 2212 26-27 0.3692402431738415 2212 28-29 0.39286484847467307 2212 30-31 0.43155138362138246 2212 32-33 0.560213392748885 2212 34-35 0.537973115246345 2212 36-37 0.6779592391022291 2212 38-39 0.5100894969228733 2212 40-41 0.49808586155210577 2212 42-43 0.4896344016103882 2212 44-45 0.42122860010402974 2212 46-47 0.5775337611864089 2212 48-49 0.4130757913957126 2212 50-51 0.4151366441141704 2212 52-53 0.5752696841146587 2212 54-55 0.2870990975327885 2212 56-57 0.3740290458818549 2212 58-59 0.5323262102527551 2212 60-61 0.5348537010575711 2212 62-63 0.5177872703656092 2212 64-65 0.23607538910671622 2212 66-67 0.5043297121238908 2212 68-69 0.4637701619488368 2212 70-71 0.45781154318166983 2212 72-73 0.5197862438404073 2212 74-75 0.5725628195960937 2212 76-77 0.46882913563280226 2212 78-79 0.44560753380924467 2212 80-81 0.35623847995135094 2212 82-83 0.6893482296027429 2212 84-85 0.6026087957681057 2212 86-87 0.34566079655905213 2212 88-89 0.4621489065477462 2212 90-91 0.5161070397406995 2212 92-93 0.5485957430631494 2212 94-95 0.6560086919761758 2212 96-97 0.43394068490647797 2212 98-99 0.3830455267756321 2212 100 0.3760840408341224 2213 1 -0.5217760734115373 2213 2 -25.822934291292974 2213 3 -14.714232487864058 2213 4 -6.138379984486878 2213 5 -2.0650199887415965 2213 6 -1.247710317759008 2213 7 -1.10807663733506 2213 8 -0.9780887933673768 2213 9 -1.1051831633789249 2213 10-11 -0.9989924416925575 2213 12-13 -1.1022128975732883 2213 14-15 -1.208471185901601 2213 16-17 -1.2969932672187028 2213 18-19 -1.2984525550297477 2213 20-21 -1.1778857036626391 2213 22-23 -1.1740047141930035 2213 24-25 -1.2141114671415636 2213 26-27 -1.3443220121621025 2213 28-29 -1.442583040081189 2213 30-31 -1.3775839856013405 2213 32-33 -1.290829262796116 2213 34-35 -1.1297362958532702 2213 36-37 -1.513202988500673 2213 38-39 -1.4727754858506472 2213 40-41 -1.2218933920025208 2213 42-43 -1.3200836280639692 2213 44-45 -1.1576142922384562 2213 46-47 -1.3012453985025587 2213 48-49 -1.267248418479383 2213 50-51 -1.2766213104676467 2213 52-53 -1.3084488895538868 2213 54-55 -1.1757556986827211 2213 56-57 -1.0958000759408435 2213 58-59 -1.2234345282993644 2213 60-61 -1.263065521137392 2213 62-63 -1.1877969367361843 2213 64-65 -1.1492170344407668 2213 66-67 -1.332689626336503 2213 68-69 -1.211565567803376 2213 70-71 -1.3571194975538958 2213 72-73 -1.3140093956727696 2213 74-75 -1.3996708099118607 2213 76-77 -1.202166107084608 2213 78-79 -1.060618799776531 2213 80-81 -1.1739398648129722 2213 82-83 -1.2530374516166631 2213 84-85 -1.3543793963578459 2213 86-87 -1.1082468950922362 2213 88-89 -1.1732731156173486 2213 90-91 -0.9970186869182172 2213 92-93 -1.0557100776747639 2213 94-95 -1.1789618683378507 2213 96-97 -1.3045557044653684 2213 98-99 -1.2003698501270144 2213 100 -1.0205282619679785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 40.0 5 34.0 6 35.0 7 43.0 8 49.0 9 97.0 10 431.0 11 1900.0 12 5561.0 13 10326.0 14 13610.0 15 15687.0 16 16971.0 17 17411.0 18 18161.0 19 19133.0 20 21230.0 21 23789.0 22 26426.0 23 27777.0 24 28110.0 25 29322.0 26 31680.0 27 36286.0 28 41962.0 29 49681.0 30 59937.0 31 73615.0 32 93336.0 33 123270.0 34 168335.0 35 236065.0 36 357351.0 37 597958.0 38 561038.0 39 2591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.18635913700344 20.955963725248406 10.63557444757038 30.222102690177778 2 12.938884957903825 23.00931539557312 35.86033764217784 28.191462004345208 3 15.438033379631388 27.08816476251607 28.91774386852188 28.556057989330654 4 10.8606027138772 19.154092132738647 36.81112600323427 33.17417915014988 5 11.617544680802293 38.81160027905976 34.93766815032699 14.633186889810954 6 30.04284380016863 39.00425557373798 17.84419717824033 13.108703447853065 7 25.74903805218537 33.35116735288872 23.166576019631453 17.733218575294455 8 24.644507062224605 35.92671134922443 21.226073531621875 18.20270805692909 9 25.003895590782033 16.654518331431696 22.030027435298113 36.31155864248816 10-11 22.786748740823892 27.609134750306485 29.132737840272956 20.471378668596664 12-13 24.062651162962442 25.212689749904914 29.16606047626257 21.55859861087007 14-15 21.97929350510277 26.88349250162707 26.42706337959907 24.710150613671086 16-17 20.67464916714697 30.01952117550929 27.832130444430742 21.473699212912997 18-19 20.837697668863324 29.005795443762928 29.742529740554485 20.413977146819263 20-21 21.899258004564647 28.412484927871606 28.94897147721995 20.739285590343794 22-23 21.18239822642112 28.229180799642627 28.722563567241583 21.86585740669467 24-25 21.398167112877907 28.377078278692764 28.48952941819272 21.735225190236612 26-27 21.317058962938024 27.972547751467186 28.83063816529232 21.879755120302466 28-29 21.076017139514487 27.824797878991543 29.38607526124506 21.71310972024891 30-31 21.794257878581348 28.43235817298285 28.637985103446645 21.135398844989155 32-33 20.74828193138282 28.2036394614759 29.181928596040297 21.86615001110098 34-35 20.780221950305723 28.136916725264054 29.29314013727477 21.789721187155454 36-37 21.790717306430913 28.251786815712236 28.49597788825938 21.46151798959747 38-39 21.65693556618025 28.221169596147792 28.639537705822693 21.48235713184926 40-41 21.32050120243551 28.086697168970336 28.73326233765275 21.859539290941406 42-43 21.594550897540486 27.846216598316026 28.721221673858555 21.838010830284933 44-45 21.988525077905884 27.716093314305667 28.448087476070842 21.847294131717607 46-47 21.656965046850555 28.185180591149077 28.72478704796838 21.433067314031984 48-49 21.789928807635892 28.054159065812044 28.463894509921627 21.69201761663043 50-51 21.51671194117243 28.062526812130862 28.371590844483364 22.049170402213345 52-53 21.311048403800296 28.102230474350083 27.960954422828348 22.625766699021277 54-55 21.674858751378242 28.442147735896324 27.655637456917486 22.227356055807952 56-57 21.901737226996836 27.732338905914496 27.938645802050594 22.427278065038077 58-59 21.405909076730065 27.394908534621397 28.680741557400484 22.51844083124805 60-61 21.902178567526143 27.93519300566717 28.04051110842043 22.12211731838626 62-63 21.209033622558252 28.088706716629908 28.151539164236155 22.55072049657569 64-65 21.01253065289159 27.786558966611075 28.528116673419458 22.672793707077876 66-67 21.588954971518017 27.758931307291608 28.103410085169067 22.54870363602131 68-69 22.02748354273351 27.67167061099563 27.899321772326687 22.401524073944167 70-71 21.542422792467097 27.725523380827482 27.99708994943409 22.73496387727133 72-73 21.73248099592783 27.703174667636404 28.13141698940055 22.432927347035218 74-75 22.112356704876238 27.510836259644922 27.71210151906076 22.664705516418078 76-77 21.588646659315238 27.804749829350655 28.219421279982694 22.387182231351414 78-79 21.885430404291075 27.8136977401156 28.139995256337723 22.160876599255598 80-81 21.524836747640528 27.721717090522624 28.13557213494163 22.617874026895223 82-83 21.185073159693683 27.768015195053447 27.957122810501655 23.08978883475121 84-85 21.330098404468853 27.697974047355594 27.709122185625727 23.262805362549823 86-87 21.699218703136907 27.554229451482758 27.7354572490473 23.01109459633303 88-89 21.34919886403381 27.28515191342235 28.23097831553718 23.134670907006658 90-91 21.45258872081091 27.40753250204868 28.033579913327873 23.10629886381254 92-93 20.947176886586565 27.70934599119907 27.956490339850703 23.386986782363664 94-95 20.7009302329015 27.486809827499787 28.296122048682754 23.516137890915964 96-97 21.31681870226049 27.380654458049765 28.017255061762846 23.285271777926898 98-99 21.33277893462063 27.22439103343131 27.86850120227377 23.574328829674283 100 21.024157666863864 27.419508007906106 27.94813364406933 23.608200681160703 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 57.0 1 41.0 2 22.5 3 22.5 4 30.5 5 45.0 6 91.5 7 201.5 8 328.0 9 363.5 10 277.5 11 220.0 12 259.0 13 319.5 14 398.0 15 480.0 16 545.5 17 633.0 18 771.0 19 937.5 20 1200.0 21 1580.0 22 2211.0 23 3269.0 24 4792.5 25 7071.0 26 10733.5 27 16013.0 28 23384.0 29 32079.0 30 41223.0 31 51139.0 32 61140.0 33 70571.0 34 79710.0 35 87561.5 36 93513.5 37 98696.5 38 102624.5 39 104920.0 40 105990.5 41 105718.5 42 103495.5 43 100370.5 44 96631.0 45 92798.5 46 90466.0 47 89438.5 48 88097.0 49 90050.0 50 108431.0 51 105952.0 52 79679.0 53 68081.5 54 62740.0 55 58294.0 56 54092.5 57 49403.5 58 44536.0 59 40024.0 60 34978.5 61 29533.5 62 24644.0 63 20040.0 64 15601.5 65 12250.0 66 9531.5 67 7406.0 68 5851.0 69 4365.5 70 3218.5 71 2409.5 72 1811.0 73 1378.0 74 958.0 75 596.5 76 370.0 77 240.0 78 165.0 79 84.0 80 32.5 81 14.5 82 12.0 83 8.0 84 6.0 85 3.5 86 3.0 87 2.5 88 1.5 89 0.5 90 1.0 91 1.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7749726216449749 2 5.269082999508721 3 0.0 4 0.005315110378971061 5 0.16462077979313147 6 0.05588247995668186 7 0.03506496430571186 8 0.04119210543702573 9 0.03938349148862585 10-11 0.04196722570062567 12-13 0.04012170126348294 14-15 0.043166816584768446 16-17 0.03381000768845481 18-19 0.02768286655714094 20-21 0.022699950576855574 22-23 0.022496942888769873 24-25 0.019378006589998657 26-27 0.012365013728856287 28-29 0.02013467160922718 30-31 0.026243357496169614 32-33 0.001384143327857047 34-35 0.0033957649643426226 36-37 0.003986332784228296 38-39 0.005887222954485307 40-41 0.006348604063770989 42-43 0.006902261394913809 44-45 0.008083397034685155 46-47 0.015908420648170326 48-49 0.02509913234514112 50-51 0.021961740801998482 52-53 0.02694465678228385 54-55 0.023862630972255494 56-57 0.015816144426313194 58-59 0.014413545854084718 60-61 0.011848266886456323 62-63 0.017181832509798812 64-65 0.018436789127055868 66-67 0.019451827567484366 68-69 0.026833925316055288 70-71 0.026317178473655323 72-73 0.02314287644176983 74-75 0.023714989017284076 76-77 0.01793849752902733 78-79 0.014007530477913317 80-81 0.012457289950713425 82-83 0.008028031301570874 84-85 0.010501034047342131 86-87 0.008692420098942256 88-89 0.007289821526713782 90-91 0.007271366282342354 92-93 0.004115519494828287 94-95 0.0015871510159427473 96-97 8.489412410856556E-4 98-99 5.721125755142461E-4 100 0.11051000329610665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2709257.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.31865776269996 #Duplication Level Percentage of deduplicated Percentage of total 1 86.65859719090442 68.7364361278102 2 9.739107226178803 15.449858259750293 3 2.173346370146988 5.171607508004856 4 0.6873607214433824 2.1808211929476076 5 0.2760261546201488 1.094701204593484 6 0.13209789550049422 0.6286696658625964 7 0.08135914220517083 0.45173085695071485 8 0.0535105853973435 0.33955102478508953 9 0.03681586315574953 0.2628166364900362 >10 0.14897944098898513 1.9883164392764128 >50 0.007406433330751232 0.39608566424599917 >100 0.0037564442555073577 0.5495260079163561 >500 5.143385884811775E-4 0.31190890621239337 >1k 0.0010286771768504832 1.5875829534285086 >5k 4.6758053493203785E-5 0.35952971897715186 >10k+ 4.6758053493203785E-5 0.4908578327483603 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 13235 0.4885103185116805 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9694 0.3578102778732324 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3881 0.14324960681101867 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3564 0.13154898187953376 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3546 0.13088459308216238 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 3296 0.12165697089644874 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 2969 0.10958724107753529 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2738 0.10106091817793587 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.382097748570918E-5 0.0 6 0.0 0.0 0.0 7.382097748570918E-5 0.0 7 0.0 0.0 0.0 7.382097748570918E-5 0.0 8 0.0 0.0 0.0 7.382097748570918E-5 0.0 9 7.382097748570918E-5 0.0 0.0 1.1073146622856377E-4 0.0 10-11 7.382097748570918E-5 0.0 0.0 1.2918671059999106E-4 0.0 12-13 7.382097748570918E-5 0.0 0.0 1.4764195497141835E-4 0.0 14-15 7.382097748570918E-5 0.0 0.0 1.4764195497141835E-4 0.0 16-17 7.382097748570918E-5 0.0 0.0 1.6609719934284565E-4 0.0 18-19 9.227622185713647E-5 0.0 0.0 1.8455244371427294E-4 0.0 20-21 1.1073146622856377E-4 0.0 0.0 1.8455244371427294E-4 0.0 22-23 1.1073146622856377E-4 0.0 0.0 2.0300768808570024E-4 0.0 24-25 1.1073146622856377E-4 0.0 0.0 3.691048874285459E-4 0.0 26-27 1.1073146622856377E-4 0.0 0.0 5.721125755142461E-4 0.0 28-29 1.1073146622856377E-4 0.0 0.0 0.0014210538165999017 0.0 30-31 1.1073146622856377E-4 0.0 0.0 0.004964460735913942 0.0 32-33 1.1073146622856377E-4 0.0 0.0 0.016480533223684573 0.0 34-35 1.1073146622856377E-4 0.0 0.0 0.034492851730197616 0.0 36-37 1.1073146622856377E-4 0.0 0.0 0.06291392806219565 0.0 38-39 1.1073146622856377E-4 0.0 0.0 0.10910740472387817 0.0 40-41 1.1073146622856377E-4 0.0 0.0 0.16995434541647397 0.0 42-43 1.1073146622856377E-4 0.0 0.0 0.2282729176301842 0.0 44-45 1.1073146622856377E-4 0.0 0.0 0.2968156952256652 0.0 46-47 1.1073146622856377E-4 0.0 0.0 0.3779818599712025 0.0 48-49 1.1073146622856377E-4 0.0 0.0 0.4628206183466537 0.0 50-51 1.1073146622856377E-4 0.0 0.0 0.5497448193360763 0.0 52-53 1.1073146622856377E-4 0.0 0.0 0.6491447655205838 0.0 54-55 1.1073146622856377E-4 0.0 0.0 0.7595071268617188 0.0 56-57 1.1073146622856377E-4 0.0 0.0 0.8867744920470816 0.0 58-59 1.1073146622856377E-4 0.0 0.0 1.0293966205494716 0.0 60-61 1.1073146622856377E-4 0.0 0.0 1.176983209787776 0.0 62-63 1.4764195497141835E-4 0.0 0.0 1.330198648559365 0.0 64-65 1.4764195497141835E-4 0.0 0.0 1.5080333833224386 0.0 66-67 1.4764195497141835E-4 0.0 0.0 1.7106350560319674 0.0 68-69 1.4764195497141835E-4 0.0 0.0 1.9417500812953516 0.0 70-71 1.4764195497141835E-4 0.0 0.0 2.2038699171027334 0.0 72-73 1.4764195497141835E-4 0.0 0.0 2.4677983668585153 0.0 74-75 2.3991817682855483E-4 0.0 0.0 2.746601743577667 0.0 76-77 2.583734211999821E-4 0.0 0.0 3.0546935931142745 0.0 78-79 2.583734211999821E-4 0.0 0.0 3.3999173943261933 0.0 80-81 2.583734211999821E-4 0.0 0.0 3.7769764920788242 0.0 82-83 2.952839099428367E-4 0.0 0.0 4.191038354796167 0.0 84-85 2.952839099428367E-4 0.0 0.0 4.612629957217052 0.0 86-87 2.952839099428367E-4 0.0 0.0 5.064285152718993 0.0 88 3.321943986856913E-4 0.0 0.0 5.448098869911566 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 5050 0.0 18.715748 4 GAGTACT 4155 0.0 17.231665 12-13 TCTGTCG 675 0.0 16.69306 8 GTCTAGA 1015 0.0 16.147625 1 CTAGACT 930 0.0 15.674051 4 CTGTCGC 760 0.0 15.443826 9 TAGCGTG 215 5.1729276E-4 15.309538 5 AATACCG 220 6.159245E-4 14.961595 5 CGCGTGA 95 0.004059248 14.826073 10-11 ATGGGGG 3205 0.0 14.818213 5 AGTACTT 5080 0.0 14.556109 12-13 GTATATA 1675 0.0 14.52921 1 GTATAGG 755 0.0 14.472263 1 GTACTAG 525 7.039489E-10 14.190336 1 GTACTTT 5380 0.0 13.744303 14-15 GTTAGAC 530 1.8408173E-9 13.307911 3 GTGTATA 1285 0.0 13.141245 1 TTATACT 1570 0.0 12.878678 4 GTACATG 21885 0.0 12.708733 1 GTGTAAG 955 0.0 12.481552 1 >>END_MODULE