FastQCFastQC Report
Wed 25 May 2016
SRR1295146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320166
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG2008.766544E-418.645562
GTACATG99400.018.324941
TACATGG98850.017.9193532
TATACTG15550.017.1655125
GTATAGG9450.017.1042041
ACATGGG101500.016.6814823
GTATAGA11200.016.651931
CATGGGG47000.016.2773634
GTAATAC11900.015.1264563
TCTATAC11600.015.0327313
CTATACT14350.014.8961724
GTCTAGG10150.014.6996351
AGTACCG3453.4164332E-614.59795955
GTCTACG3007.7867863E-414.5056811
GTATTAG12150.014.3265991
GTATCAA96450.014.18018151
GTACCGT2550.001120669913.8241216
GTCTAGT9050.013.7386081
CATGGGT20700.013.3158454
GTATTAT17850.013.2344751
Produced by FastQC (version 0.11.5)