##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295146_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3320166 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.131644622588148 33.0 27.0 33.0 6.0 33.0 2 22.22327618558831 27.0 2.0 33.0 2.0 33.0 3 24.474237432706676 27.0 15.0 33.0 15.0 33.0 4 24.43670105651344 33.0 6.0 37.0 2.0 37.0 5 26.350753245470255 33.0 15.0 37.0 2.0 37.0 6 27.332849923768872 33.0 15.0 37.0 2.0 37.0 7 27.08101974419351 33.0 15.0 37.0 6.0 37.0 8 27.735636109760776 33.0 15.0 37.0 6.0 37.0 9 28.757737715523863 33.0 22.0 37.0 15.0 37.0 10-11 28.317745709100087 33.0 18.5 37.0 10.5 37.0 12-13 27.089195992007628 33.0 15.0 37.0 4.0 37.0 14-15 28.841185199776156 33.0 22.0 37.0 4.0 40.0 16-17 29.27577160298612 33.0 22.0 37.0 6.0 40.0 18-19 29.46326915581932 33.0 22.0 40.0 6.0 40.0 20-21 28.970517437983524 33.0 22.0 38.5 6.0 40.0 22-23 29.73040429303836 33.0 27.0 38.5 6.0 40.0 24-25 29.85150938236221 33.0 27.0 38.5 6.0 40.0 26-27 28.41873418377274 33.0 18.5 37.0 4.0 40.0 28-29 29.108139020759808 33.0 22.0 37.0 6.0 40.0 30-31 29.855948467636857 33.0 27.0 38.5 10.5 40.0 32-33 28.558754441795983 33.0 22.0 37.0 4.0 40.0 34-35 28.230283666539563 33.0 22.0 37.0 6.0 40.0 36-37 28.50214266395114 33.0 22.0 37.0 6.0 40.0 38-39 28.622042241261433 33.0 22.0 37.0 6.0 40.0 40-41 27.754554440952653 33.0 15.0 37.0 6.0 40.0 42-43 28.337391262966975 33.0 22.0 37.0 6.0 40.0 44-45 28.24238622406229 33.0 22.0 37.0 6.0 40.0 46-47 27.922370447742672 33.0 22.0 37.0 6.0 40.0 48-49 27.861085710774702 33.0 18.5 37.0 6.0 40.0 50-51 27.751936348965685 33.0 22.0 37.0 6.0 40.0 52-53 27.990692332853236 33.0 22.0 37.0 6.0 40.0 54-55 27.933487060586728 33.0 22.0 37.0 6.0 40.0 56-57 27.928568481214494 33.0 22.0 37.0 6.0 40.0 58-59 27.79203328990177 33.0 22.0 37.0 6.0 40.0 60-61 27.75250107976529 33.0 22.0 37.0 6.0 40.0 62-63 27.74987124137769 33.0 22.0 37.0 6.0 40.0 64-65 27.304569560678594 33.0 22.0 37.0 6.0 40.0 66-67 27.48928833678798 33.0 22.0 37.0 6.0 38.5 68-69 27.041808150556328 33.0 22.0 37.0 6.0 37.0 70-71 27.196547100355826 33.0 22.0 37.0 6.0 37.0 72-73 27.248022990416743 33.0 22.0 37.0 10.5 37.0 74-75 27.37248318306976 30.0 22.0 37.0 10.5 37.0 76-77 27.12612577202465 27.0 22.0 37.0 6.0 37.0 78-79 26.69287198290688 27.0 22.0 33.0 6.0 37.0 80-81 26.52493836151566 27.0 22.0 33.0 6.0 37.0 82-83 26.618287007336377 27.0 22.0 33.0 10.5 37.0 84-85 26.57539848911169 27.0 22.0 33.0 6.0 37.0 86-87 26.39250130866951 27.0 22.0 33.0 10.5 37.0 88-89 26.34986262735056 27.0 22.0 33.0 6.0 37.0 90-91 26.48874227975348 27.0 22.0 33.0 15.0 37.0 92-93 26.400787942530584 27.0 22.0 33.0 6.0 37.0 94-95 26.271237040557608 27.0 22.0 33.0 10.5 37.0 96-97 26.36768568198096 27.0 22.0 33.0 10.5 37.0 98-99 26.4490100796165 27.0 22.0 33.0 15.0 37.0 100 26.104951680126838 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.253395137067535 1101 2 11.237823354742062 1101 3 8.45060898086032 1101 4 12.729408518340247 1101 5 10.798164883391873 1101 6 10.017054646611285 1101 7 9.50918888922918 1101 8 9.010132755857434 1101 9 7.3305473368628356 1101 10-11 7.775027456323521 1101 12-13 9.118256933554715 1101 14-15 8.009622361516744 1101 16-17 8.286116444515528 1101 18-19 8.178856069104615 1101 20-21 7.259548128957835 1101 22-23 7.409944210691567 1101 24-25 7.359373318394297 1101 26-27 8.524916891656066 1101 28-29 8.141259925658822 1101 30-31 5.428518188969832 1101 32-33 6.878504408053651 1101 34-35 8.565267210074222 1101 36-37 8.245896375031585 1101 38-39 8.206972585471465 1101 40-41 9.017216858967235 1101 42-43 8.579325202378499 1101 44-45 8.60918898450797 1101 46-47 7.357392532295428 1101 48-49 5.410362370303652 1101 50-51 6.678641972947936 1101 52-53 5.896325338580876 1101 54-55 4.1092810433374325 1101 56-57 6.152490918010006 1101 58-59 6.102665601363707 1101 60-61 6.676202744090524 1101 62-63 6.209412525616962 1101 64-65 6.62375217431892 1101 66-67 5.8585580423754955 1101 68-69 6.7428121562388945 1101 70-71 6.373646647672235 1101 72-73 5.5364303558935966 1101 74-75 4.939084369931038 1101 76-77 5.070351136720596 1101 78-79 5.110980798475538 1101 80-81 4.9195969691212404 1101 82-83 4.471289910050839 1101 84-85 4.193854309633238 1101 86-87 4.067424938716371 1101 88-89 4.132562441158353 1101 90-91 3.886648456338669 1101 92-93 3.779911501982518 1101 94-95 3.6773800106817696 1101 96-97 3.8339433482457395 1101 98-99 3.6264115173745033 1101 100 3.8483994846977865 1102 1 6.654336852451699 1102 2 10.356505665016162 1102 3 8.088376974610533 1102 4 12.374844417611389 1102 5 10.433402012131321 1102 6 9.463260049971709 1102 7 6.454046293833105 1102 8 7.647194797345847 1102 9 6.332675398907668 1102 10-11 7.313916268743974 1102 12-13 9.004176556223602 1102 14-15 6.223772671420381 1102 16-17 7.334605102618298 1102 18-19 7.329537570036752 1102 20-21 6.8909531827832105 1102 22-23 6.53948824992538 1102 24-25 6.943593018163035 1102 26-27 8.463811121319228 1102 28-29 7.576052604535484 1102 30-31 4.124436950385615 1102 32-33 4.369080352389169 1102 34-35 5.047745526847436 1102 36-37 5.2549121711607825 1102 38-39 4.57240688021626 1102 40-41 6.19232809924446 1102 42-43 6.893092510272361 1102 44-45 6.376314933729827 1102 46-47 5.416598069563001 1102 48-49 3.6670363641761696 1102 50-51 4.974350218935193 1102 52-53 5.794713979242289 1102 54-55 5.2905346503264035 1102 56-57 6.284254198070837 1102 58-59 5.9907829630268985 1102 60-61 6.0413098806343335 1102 62-63 4.3528762142617765 1102 64-65 6.055608589661578 1102 66-67 3.8692808500291633 1102 68-69 6.066629884799838 1102 70-71 5.294614886899247 1102 72-73 4.967626241227382 1102 74-75 4.271788047934066 1102 76-77 3.273817847616609 1102 78-79 4.278589039605958 1102 80-81 4.474177370736545 1102 82-83 3.9251880536964485 1102 84-85 3.0043492883113245 1102 86-87 3.220954100294339 1102 88-89 3.5006606723843596 1102 90-91 3.2989539640522914 1102 92-93 3.30650940669922 1102 94-95 3.1561661176859808 1102 96-97 3.2575892194514324 1102 98-99 3.0669864293860947 1102 100 3.238933093920508 1103 1 6.5878005908992705 1103 2 10.871764223902257 1103 3 7.833292325670879 1103 4 11.69929101457615 1103 5 10.190737429865553 1103 6 8.951628936906829 1103 7 7.885772333444059 1103 8 8.032878160629274 1103 9 7.019816275448534 1103 10-11 7.349056798729514 1103 12-13 8.698398796631754 1103 14-15 8.547804942684362 1103 16-17 8.03808547252008 1103 18-19 7.136223696758751 1103 20-21 8.295627857972637 1103 22-23 6.864711154831358 1103 24-25 5.170416050246018 1103 26-27 5.879976076452127 1103 28-29 6.972586100848531 1103 30-31 6.020680446720668 1103 32-33 7.864324380762689 1103 34-35 7.041755041912094 1103 36-37 4.056689667834789 1103 38-39 6.323096199169019 1103 40-41 6.818967759140026 1103 42-43 7.461669286383405 1103 44-45 5.669667644414755 1103 46-47 6.4068153149323805 1103 48-49 5.782899984250687 1103 50-51 6.477189921689966 1103 52-53 6.2615003729039245 1103 54-55 5.100242541403183 1103 56-57 5.958991785457503 1103 58-59 5.5028993235796975 1103 60-61 5.032319191007247 1103 62-63 2.728539306459698 1103 64-65 5.0039294170564155 1103 66-67 4.227822968606709 1103 68-69 5.276362597001448 1103 70-71 3.8643724722489985 1103 72-73 4.626407266883081 1103 74-75 3.316403254469634 1103 76-77 3.5724892421356316 1103 78-79 3.1248744547290137 1103 80-81 4.237776185725423 1103 82-83 3.681854761714984 1103 84-85 3.5384071730552655 1103 86-87 2.950943341472229 1103 88-89 3.191177121857013 1103 90-91 2.82069240722252 1103 92-93 2.473290417948462 1103 94-95 2.99489717694674 1103 96-97 2.9855180571564937 1103 98-99 2.825725996460058 1103 100 2.9649159994587713 1104 1 6.368970498260641 1104 2 9.9002134462518 1104 3 7.422980388156443 1104 4 12.155285774651016 1104 5 9.501022242449434 1104 6 8.187326898528468 1104 7 4.042341522466366 1104 8 6.580947627446317 1104 9 6.678490655049089 1104 10-11 7.105803759310305 1104 12-13 7.53527061482902 1104 14-15 7.345789583111131 1104 16-17 7.286053379401565 1104 18-19 6.910434343798112 1104 20-21 7.625415483426455 1104 22-23 6.548998611933353 1104 24-25 6.030848322115872 1104 26-27 8.255038735773486 1104 28-29 7.254657692614423 1104 30-31 6.350154501147301 1104 32-33 7.7902419144690285 1104 34-35 8.099021169272913 1104 36-37 6.742559697136386 1104 38-39 7.400802823693386 1104 40-41 7.231837030390878 1104 42-43 7.347932627177997 1104 44-45 6.873497613869123 1104 46-47 6.753088267351785 1104 48-49 5.544206957886573 1104 50-51 5.746915462275766 1104 52-53 6.026140131303407 1104 54-55 4.92452504999417 1104 56-57 6.108960168163602 1104 58-59 3.911694448149884 1104 60-61 4.336444546564589 1104 62-63 4.0132874523739375 1104 64-65 2.8835317416694686 1104 66-67 3.3919263794772867 1104 68-69 4.3578519134296165 1104 70-71 2.7528527232099016 1104 72-73 2.5993625077392117 1104 74-75 3.220280786058673 1104 76-77 3.977153323531887 1104 78-79 4.453387610930154 1104 80-81 3.291581140237092 1104 82-83 3.0743364684455337 1104 84-85 3.116524554078172 1104 86-87 0.9665076119868239 1104 88-89 0.44503811504415225 1104 90-91 2.3421261638896134 1104 92-93 2.677280709890283 1104 94-95 2.6149479849663884 1104 96-97 2.9299224588106902 1104 98-99 2.628449876944817 1104 100 0.9358824039801128 1105 1 5.734176318387391 1105 2 -2.790516176878164 1105 3 0.4793888022946895 1105 4 8.187038226779446 1105 5 8.513924797082563 1105 6 8.009778860917926 1105 7 5.246744273991329 1105 8 5.954243334384351 1105 9 4.851503395314012 1105 10-11 4.854787936832331 1105 12-13 5.036284428415808 1105 14-15 5.874147952080076 1105 16-17 6.041001596131409 1105 18-19 6.111940919944413 1105 20-21 7.006975061501908 1105 22-23 2.110008729570332 1105 24-25 4.083900012321074 1105 26-27 6.9006507229502 1105 28-29 5.994458634046431 1105 30-31 5.294522246718358 1105 32-33 7.061594008932836 1105 34-35 6.097114342056205 1105 36-37 6.256297776263324 1105 38-39 4.10717881883761 1105 40-41 5.407400867725627 1105 42-43 5.484610193787599 1105 44-45 6.074972508981428 1105 46-47 5.4003044976346715 1105 48-49 5.135929711223639 1105 50-51 5.9933468003223425 1105 52-53 5.561284616702352 1105 54-55 5.293044054412292 1105 56-57 5.091546986598583 1105 58-59 1.9049575700528862 1105 60-61 4.331395752032549 1105 62-63 -0.916369234271631 1105 64-65 -0.5696446535310855 1105 66-67 0.6894821431193456 1105 68-69 2.859280901278492 1105 70-71 3.0327425847255505 1105 72-73 2.5917896849211566 1105 74-75 2.7384366218978258 1105 76-77 1.8585051658593486 1105 78-79 3.2091489263685062 1105 80-81 1.593616802975422 1105 82-83 1.0537657301595758 1105 84-85 1.4353552543632908 1105 86-87 -0.21117036808538714 1105 88-89 1.5803455768654828 1105 90-91 1.3736172859229896 1105 92-93 1.6843073397928734 1105 94-95 1.0613970443030887 1105 96-97 2.073544864753071 1105 98-99 1.8647701069095604 1105 100 1.9956695066116978 1106 1 6.125798974467202 1106 2 10.274893285315724 1106 3 6.733666969893157 1106 4 11.599692236963936 1106 5 9.899510598532029 1106 6 9.281854649879108 1106 7 6.5039769060235955 1106 8 7.249523462181138 1106 9 6.801350309608786 1106 10-11 5.612092229862128 1106 12-13 7.859249934205213 1106 14-15 7.850092447453445 1106 16-17 7.80860787295768 1106 18-19 7.212390524186684 1106 20-21 8.17302855575139 1106 22-23 6.14341705236242 1106 24-25 4.986764044042147 1106 26-27 7.614633041062557 1106 28-29 6.1269301041629305 1106 30-31 5.293498880920609 1106 32-33 7.285606529052224 1106 34-35 6.803851604344121 1106 36-37 5.944428772405601 1106 38-39 7.009667527091704 1106 40-41 6.643195959189121 1106 42-43 6.104726761806198 1106 44-45 4.570780542147009 1106 46-47 5.946644214694647 1106 48-49 5.815120534424306 1106 50-51 6.497448878551722 1106 52-53 5.64213205636333 1106 54-55 5.301536154674011 1106 56-57 4.982611781158635 1106 58-59 4.967486495925872 1106 60-61 4.931108997205655 1106 62-63 5.009845088133936 1106 64-65 5.153714685462713 1106 66-67 3.0061597893417407 1106 68-69 3.275213281187373 1106 70-71 3.100856735696894 1106 72-73 2.938404035297893 1106 74-75 2.9212353727877947 1106 76-77 3.6823618595829473 1106 78-79 4.211493375553179 1106 80-81 3.611843302107971 1106 82-83 2.2358674891791175 1106 84-85 2.7532866053040834 1106 86-87 3.38733006105436 1106 88-89 3.3915629009317882 1106 90-91 3.289540798891796 1106 92-93 3.027869759927441 1106 94-95 1.5446762624324357 1106 96-97 1.5508002465322797 1106 98-99 2.0469907030782757 1106 100 2.964245030673048 1107 1 6.061627670253543 1107 2 10.388049970451796 1107 3 6.044678171901715 1107 4 11.273198521382017 1107 5 9.39581362065896 1107 6 8.144021596723896 1107 7 3.78244783629842 1107 8 6.299621531254402 1107 9 5.650235399959165 1107 10-11 4.584851828150526 1107 12-13 6.184875265915373 1107 14-15 6.2360010768048575 1107 16-17 7.081279035399319 1107 18-19 6.294697074053669 1107 20-21 5.578035591843882 1107 22-23 5.762578872795846 1107 24-25 4.657469139675655 1107 26-27 7.207026285929079 1107 28-29 6.40803090527578 1107 30-31 5.048654393913139 1107 32-33 4.988598766731592 1107 34-35 6.631958612728457 1107 36-37 6.4939247345286475 1107 38-39 6.4208335271741 1107 40-41 7.721197204036255 1107 42-43 6.150032896454874 1107 44-45 4.37319335235912 1107 46-47 3.4698618052489962 1107 48-49 4.1323506606943 1107 50-51 4.732508471266179 1107 52-53 4.028814534715835 1107 54-55 5.22276657418184 1107 56-57 5.3234994557179505 1107 58-59 3.157821158657928 1107 60-61 2.9763517787483096 1107 62-63 0.5456984829475857 1107 64-65 2.115839605589734 1107 66-67 4.09315038957455 1107 68-69 4.994343758292608 1107 70-71 4.335193270950466 1107 72-73 2.5001516237599937 1107 74-75 2.7252132490834313 1107 76-77 3.105819149855286 1107 78-79 3.936954962608059 1107 80-81 2.41758357416483 1107 82-83 1.8846867625793742 1107 84-85 2.4805123047328763 1107 86-87 2.372029205494595 1107 88-89 2.457388737043342 1107 90-91 1.4541208588803407 1107 92-93 2.3220986090375852 1107 94-95 2.314729449152235 1107 96-97 2.2864876953873186 1107 98-99 1.7925947811037766 1107 100 0.05021767958011125 1108 1 4.349434821508076 1108 2 -3.277453591602642 1108 3 0.04404337808068348 1108 4 6.565071262715218 1108 5 6.969172720691105 1108 6 6.871701041489597 1108 7 4.231050911966122 1108 8 5.0252457492421385 1108 9 4.920416873537459 1108 10-11 3.4266134571986484 1108 12-13 2.4601516192461403 1108 14-15 4.004444842427322 1108 16-17 4.717410186297872 1108 18-19 3.996257796810795 1108 20-21 4.692077504653501 1108 22-23 4.08192176711594 1108 24-25 2.9814729286888983 1108 26-27 4.808204450013182 1108 28-29 6.087942968390244 1108 30-31 4.52546656955203 1108 32-33 4.42767806577227 1108 34-35 3.025569610619794 1108 36-37 1.444355021602071 1108 38-39 2.7132691852672615 1108 40-41 5.386248199668163 1108 42-43 3.154303135511377 1108 44-45 3.6978480986427407 1108 46-47 4.6586124407133305 1108 48-49 4.71288786629297 1108 50-51 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2213 44-45 -17.244595499130824 2213 46-47 -17.065579511577106 2213 48-49 -6.4327736628272625 2213 50-51 -23.429884078877087 2213 52-53 -17.838957154914663 2213 54-55 -17.76635997285204 2213 56-57 -17.655871132437927 2213 58-59 -18.07943227916629 2213 60-61 -19.37336612805526 2213 62-63 -17.68302203162779 2213 64-65 -17.674911658052775 2213 66-67 -8.6966464046628 2213 68-69 -22.689968562624298 2213 70-71 -17.401191792364074 2213 72-73 -9.283979627856233 2213 74-75 -4.469130529184305 2213 76-77 -3.2376694693134453 2213 78-79 -4.629370846855313 2213 80-81 -3.323001912665976 2213 82-83 -3.365279251226962 2213 84-85 -3.2603279097877724 2213 86-87 -3.3945317811591416 2213 88-89 -2.93676958409581 2213 90-91 -2.876299770245904 2213 92-93 -3.871678178781597 2213 94-95 -3.7702171335866232 2213 96-97 -3.455746005000371 2213 98-99 -3.7725397201014452 2213 100 -3.3523767989226485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 977.0 3 1044.0 4 7044.0 5 21598.0 6 25112.0 7 31004.0 8 30135.0 9 31212.0 10 36700.0 11 43753.0 12 54043.0 13 70675.0 14 56756.0 15 60858.0 16 68150.0 17 70436.0 18 69573.0 19 69140.0 20 68262.0 21 66007.0 22 69525.0 23 75667.0 24 82121.0 25 89701.0 26 98051.0 27 105748.0 28 113284.0 29 118436.0 30 124980.0 31 135678.0 32 154467.0 33 186176.0 34 223427.0 35 260600.0 36 281927.0 37 242889.0 38 74838.0 39 172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.447066064901314 20.72307246416861 13.957094116799459 23.872767354130623 2 15.023864626888653 21.245761407073434 40.452755070248195 23.277618895789715 3 17.831082681064647 27.255754922905297 29.529689059629753 25.383473336400304 4 12.600221390838904 17.889660887685185 39.07540299738375 30.434714724092167 5 13.241036427288671 38.168712921101 35.386668716414995 13.203581935195343 6 30.682440854373798 38.189886364822776 18.35342321719471 12.774249563608722 7 26.267653210431824 32.45731631538728 23.817555507960307 17.457474966220595 8 24.455073849533438 36.14356455021658 21.66807238448663 17.733289215763353 9 24.609405977319646 17.430022434774155 22.136560495867347 35.82401109203885 10-11 23.4090547491205 27.524108136675913 28.930137799552853 20.136699314650734 12-13 24.374625910626502 25.128319114054715 28.923163037996215 21.57389193732257 14-15 21.68891712605907 26.583076466702167 26.412268232822182 25.315738174416573 16-17 21.276235517855664 30.089295635054743 27.19580119612563 21.43866765096396 18-19 21.229778506107337 29.20784420673629 28.696839314861577 20.8655379722948 20-21 22.686165009619568 28.435706116655478 27.70721302110537 21.170915852619583 22-23 21.66123026951206 28.45180938207402 28.264109376012442 21.622850972401476 24-25 21.977564723537828 28.28611547514229 27.83086549768179 21.9054543036381 26-27 21.937215341190534 28.25592025611792 27.91928392672462 21.887580475966924 28-29 21.684926252241297 28.207722948059555 28.318074813743817 21.789275985955335 30-31 22.246449140879697 28.53343183287728 27.666154077732802 21.553964948510217 32-33 21.49021982555251 28.42015487531483 28.235091914437362 21.854533384695298 34-35 21.71131018408559 27.83593075061923 28.111990619543402 22.34076844575178 36-37 22.12379631860029 28.413555911984297 27.740307064012903 21.722340705402505 38-39 21.833304212882272 28.19301712556787 27.99866029791915 21.975018363630703 40-41 22.066624507688783 28.17162216329067 27.65263402500372 22.109119304016822 42-43 21.696842849314713 28.361293857871477 28.090340487207005 21.851522805606805 44-45 22.208551992634927 27.80257307500273 27.960446940881894 22.028427991480452 46-47 22.127784885637208 28.251818840549358 28.011433218322406 21.608963055491028 48-49 21.98017316273681 27.992279938234464 28.0550597778734 21.972487121155325 50-51 22.167256646506427 28.018893424559018 27.864278391477665 21.949571537456887 52-53 21.71800503655773 28.15804508446189 27.90091105655729 22.22303882242309 54-55 22.073973648688426 28.082093108478084 27.740541030361264 22.103392212472226 56-57 22.251126243039206 28.052225321217318 27.580231878999495 22.116416556743985 58-59 21.964925451664126 27.60722851071382 27.998730958487577 22.429115079134476 60-61 22.328853685807555 28.015948098747884 27.671438351871934 21.983759863572626 62-63 21.815423165532813 28.164791241743796 27.9927656394984 22.02701995322499 64-65 21.905063950210973 27.72032508591 27.949212685529044 22.425398278349984 66-67 21.992658022235144 27.929702262361555 27.691824417686618 22.385815297716686 68-69 22.42130446596427 27.799943483971923 27.67747079113334 22.101281258930467 70-71 22.525293636812542 27.82465888966832 27.45461962443378 22.195427849085355 72-73 22.144281877164897 27.945665019659494 27.90626628514255 22.00378681803306 74-75 22.315356800429136 27.70936578509718 27.568313247626342 22.40696416684735 76-77 22.32366979935076 27.968802543253684 27.610722653401126 22.096805003994433 78-79 22.273165715483064 27.762589120176035 27.79904292254727 22.165202241793633 80-81 22.50239496426095 27.69004251495224 27.5614476207863 22.246114900000507 82-83 21.97546891005622 27.904829763691417 27.972126582517248 22.147574743735113 84-85 22.153347479063694 27.55227067392075 27.598246589508413 22.696135257507148 86-87 22.290212515790877 27.817269901199726 27.646412061037783 22.24610552197161 88-89 22.083385536762997 27.48094208255736 27.94456921022 22.491103170459645 90-91 22.388804766634525 27.500704823957182 27.602643671526955 22.507846737881334 92-93 22.025179162333362 27.792122530017966 27.866714569348588 22.315983738300087 94-95 21.984810600870393 27.354074939946916 27.84677701964503 22.814337439537667 96-97 22.106040369985188 27.571781603785528 27.875807794572456 22.446370231656832 98-99 22.057988757238533 27.457621784983548 27.82869403506691 22.65569542271101 100 22.240504156942073 27.132058630740698 27.62673200866618 23.00070520365105 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 421.0 1 481.5 2 464.5 3 382.0 4 435.5 5 596.5 6 914.0 7 1404.0 8 2095.5 9 3011.5 10 4092.0 11 5202.0 12 6281.5 13 7392.0 14 8490.0 15 9461.0 16 10312.5 17 11222.0 18 12306.5 19 13478.5 20 14650.0 21 15872.0 22 17224.5 23 18952.5 24 20892.0 25 23496.5 26 27430.0 27 32921.0 28 39862.0 29 48040.0 30 57025.0 31 66462.0 32 76388.5 33 85945.5 34 94922.0 35 102889.5 36 109891.5 37 116300.5 38 121022.0 39 123930.0 40 125165.5 41 125426.5 42 124258.5 43 120865.5 44 116486.5 45 112366.0 46 108767.5 47 105119.5 48 101683.0 49 98557.5 50 95535.5 51 89741.5 52 81113.0 53 73822.5 54 67957.5 55 62739.5 56 57800.5 57 52679.5 58 47557.5 59 42367.5 60 36880.0 61 31213.5 62 26411.0 63 21923.0 64 17611.0 65 14291.0 66 11444.0 67 9051.5 68 7043.0 69 5373.0 70 4215.5 71 3235.5 72 2400.0 73 1830.5 74 1415.5 75 1049.0 76 736.5 77 501.5 78 348.0 79 238.5 80 170.0 81 126.0 82 83.5 83 57.5 84 39.5 85 30.0 86 25.0 87 14.5 88 10.0 89 8.0 90 5.5 91 5.0 92 2.0 93 2.0 94 2.5 95 2.0 96 1.0 97 0.5 98 0.5 99 1.5 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.44665598045399 2 25.46809406517626 3 6.640390872022664 4 24.76746644595481 5 13.795274091717099 6 10.569561883351614 7 8.874676748090305 8 9.974892821623978 9 5.809287848860569 10-11 7.335762729935793 12-13 11.108857207742021 14-15 8.316120338561385 16-17 8.65038073397535 18-19 7.230316195033621 20-21 8.629282391302121 22-23 6.337529509066715 24-25 5.792601936168252 26-27 10.404374359595273 28-29 6.915241587318224 30-31 4.556549280969686 32-33 9.576162758127154 34-35 7.6824472029410575 36-37 7.723710200032167 38-39 6.655375062572172 40-41 8.816923611650743 42-43 7.109764993678026 44-45 7.071679548552693 46-47 6.517836758764471 48-49 4.384720523009994 50-51 7.236339990229404 52-53 4.922841207337224 54-55 5.604855299403705 56-57 5.423825194282455 58-59 4.116089376254079 60-61 4.601170543882445 62-63 3.3359777794242818 64-65 4.841775983489982 66-67 2.9683606181136724 68-69 5.725030013559563 70-71 2.8359124212464075 72-73 1.8043977319206328 74-75 0.9540185641320343 76-77 1.2982483405950185 78-79 1.96862144844565 80-81 2.3319466556792645 82-83 1.001004166659137 84-85 1.4397021112799782 86-87 0.5070529606049817 88-89 0.7158377020907991 90-91 0.3261583908756369 92-93 0.44737220970276786 94-95 0.645088227516335 96-97 0.6506150596084653 98-99 0.46033842886168946 100 0.6574370076676889 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3320166.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.07321700207034 #Duplication Level Percentage of deduplicated Percentage of total 1 97.09873263608361 92.31488878536388 2 2.282266011214634 4.339647434813168 3 0.31717151701989604 0.9046354939352528 4 0.11721054419219956 0.44574334011662975 5 0.05214812997678266 0.24789452387674135 6 0.03867173471151368 0.22059877356445418 7 0.021560344616689674 0.14348679256673696 8 0.013781005021637637 0.1048163584743021 9 0.010469539579783502 0.0895835527542469 >10 0.045341372857816226 0.6898002041285972 >50 0.0011694700369331889 0.07756177996071875 >100 0.0012866174830210011 0.2556055263862955 >500 1.2738480442723145E-4 0.07072755757056008 >1k 6.369240096982764E-5 0.09500987648837271 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.0118975978911897E-5 0.0 0.0 0.0 3 0.0 3.0118975978911897E-5 0.0 0.0 0.0 4 0.0 3.0118975978911897E-5 0.0 0.0 0.0 5 0.0 6.0237951957823794E-5 0.0 0.0 0.0 6 0.0 6.0237951957823794E-5 0.0 0.0 0.0 7 0.0 6.0237951957823794E-5 0.0 0.0 0.0 8 0.0 6.0237951957823794E-5 0.0 0.0 0.0 9 0.0 6.0237951957823794E-5 0.0 3.0118975978911897E-5 0.0 10-11 1.5059487989455948E-5 6.0237951957823794E-5 0.0 4.517846396836784E-5 0.0 12-13 3.0118975978911897E-5 6.0237951957823794E-5 0.0 7.529743994727975E-5 0.0 14-15 3.0118975978911897E-5 7.529743994727975E-5 0.0 1.2047590391564759E-4 0.0 16-17 4.517846396836784E-5 1.0541641592619165E-4 0.0 1.3553539190510353E-4 0.0 18-19 6.0237951957823794E-5 1.505948798945595E-4 0.0 1.807138558734714E-4 0.0 20-21 6.0237951957823794E-5 1.505948798945595E-4 0.0 1.807138558734714E-4 0.0 22-23 6.0237951957823794E-5 1.807138558734714E-4 0.0 2.1083283185238327E-4 0.0 24-25 6.0237951957823794E-5 1.807138558734714E-4 0.0 2.8613027179966307E-4 0.0 26-27 6.0237951957823794E-5 1.807138558734714E-4 0.0 4.517846396836785E-4 0.0 28-29 6.0237951957823794E-5 1.807138558734714E-4 0.0 7.379149114833416E-4 0.0 30-31 6.0237951957823794E-5 1.807138558734714E-4 0.0 0.0019125549746609055 0.0 32-33 6.0237951957823794E-5 1.807138558734714E-4 0.0 0.005602129532077613 0.0 34-35 7.529743994727975E-5 1.807138558734714E-4 0.0 0.011038604696271211 0.0 36-37 9.03569279367357E-5 1.807138558734714E-4 0.0 0.01888459793877776 0.0 38-39 9.03569279367357E-5 1.807138558734714E-4 0.0 0.032438137129288114 0.0 40-41 9.03569279367357E-5 2.1083283185238327E-4 0.0 0.04859696774197435 0.0 42-43 9.03569279367357E-5 2.1083283185238327E-4 0.0 0.06522264248233371 0.0 44-45 9.03569279367357E-5 2.1083283185238327E-4 0.0 0.08614027129968803 0.0 46-47 9.03569279367357E-5 2.1083283185238327E-4 0.0 0.11293110043293016 0.0 48-49 9.03569279367357E-5 2.1083283185238327E-4 0.0 0.14064055833352912 0.0 50-51 9.03569279367357E-5 2.1083283185238327E-4 0.0 0.17143721127196654 0.0 52-53 9.03569279367357E-5 2.1083283185238327E-4 0.0 0.20130017595505767 0.0 54-55 1.0541641592619165E-4 2.1083283185238327E-4 0.0 0.2382410999931931 0.0 56-57 1.2047590391564759E-4 3.01189759789119E-4 0.0 0.28222986441039394 0.0 58-59 1.2047590391564759E-4 3.01189759789119E-4 0.0 0.3298329059450642 0.0 60-61 1.2047590391564759E-4 3.01189759789119E-4 0.0 0.38287242264392807 0.0 62-63 1.2047590391564759E-4 3.01189759789119E-4 0.0 0.43750824506967423 0.0 64-65 1.2047590391564759E-4 3.01189759789119E-4 0.0 0.5013152956809991 0.0 66-67 1.2047590391564759E-4 3.162492477785749E-4 0.0 0.5779680895473298 0.0 68-69 1.2047590391564759E-4 3.3130873576803086E-4 0.0 0.6660660942856471 0.0 70-71 1.505948798945595E-4 3.3130873576803086E-4 0.0 0.7694344198452727 0.0 72-73 1.505948798945595E-4 3.3130873576803086E-4 0.0 0.8781488636411553 1.5059487989455948E-5 74-75 1.505948798945595E-4 3.3130873576803086E-4 0.0 0.9916221056417059 6.0237951957823794E-5 76-77 1.505948798945595E-4 3.3130873576803086E-4 0.0 1.1179109719212836 6.0237951957823794E-5 78-79 1.807138558734714E-4 3.3130873576803086E-4 0.0 1.265900560393667 6.0237951957823794E-5 80-81 2.1083283185238327E-4 3.3130873576803086E-4 0.0 1.4341451602118689 6.0237951957823794E-5 82-83 2.1083283185238327E-4 3.3130873576803086E-4 0.0 1.6149192540372983 6.0237951957823794E-5 84-85 2.1083283185238327E-4 3.3130873576803086E-4 0.0 1.8015364292026361 6.0237951957823794E-5 86-87 2.1083283185238327E-4 3.3130873576803086E-4 0.0 2.0073092730905624 6.0237951957823794E-5 88 2.1083283185238327E-4 3.614277117469428E-4 0.0 2.178113985866972 6.0237951957823794E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACGG 200 8.766544E-4 18.64556 2 GTACATG 9940 0.0 18.32494 1 TACATGG 9885 0.0 17.919353 2 TATACTG 1555 0.0 17.165512 5 GTATAGG 945 0.0 17.104204 1 ACATGGG 10150 0.0 16.681482 3 GTATAGA 1120 0.0 16.65193 1 CATGGGG 4700 0.0 16.277363 4 GTAATAC 1190 0.0 15.126456 3 TCTATAC 1160 0.0 15.032731 3 CTATACT 1435 0.0 14.896172 4 GTCTAGG 1015 0.0 14.699635 1 AGTACCG 345 3.4164332E-6 14.5979595 5 GTCTACG 300 7.7867863E-4 14.505681 1 GTATTAG 1215 0.0 14.326599 1 GTATCAA 9645 0.0 14.1801815 1 GTACCGT 255 0.0011206699 13.824121 6 GTCTAGT 905 0.0 13.738608 1 CATGGGT 2070 0.0 13.315845 4 GTATTAT 1785 0.0 13.234475 1 >>END_MODULE