FastQCFastQC Report
Wed 25 May 2016
SRR1295146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320166
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50050.15074547477445405No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38210.11508460721542237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG2151.5594336E-518.5795151
GTATAGG10800.018.0311621
GTATCAA132800.016.6190831
CATGGGG57950.016.3866674
GTACATG139700.016.0484391
ACATGGG135300.015.9473293
TACATGG138000.015.6895642
GTATATA24100.015.5391441
CAACGCA145850.014.9878465
TATCAAC146700.014.9631422
ATCAACG146650.014.8733563
TCAACGC147300.014.8403094
AACGCAG148200.014.7957696
CTGGTCG3859.3279596E-814.6326829
GAGTACT42000.014.58717312-13
CCTATAC11600.014.183493
TATACCG2000.00565649214.1030815
AGTACTT51350.013.89674612-13
ACGCAGA158650.013.6717777
CGCAGAG159900.013.5061758
Produced by FastQC (version 0.11.5)