##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295146_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3320166 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.235625568119186 33.0 33.0 33.0 27.0 33.0 2 29.850913478422463 33.0 33.0 33.0 22.0 33.0 3 30.720897087675738 33.0 33.0 33.0 27.0 33.0 4 34.99270518401791 37.0 37.0 37.0 33.0 37.0 5 35.35680264179562 37.0 37.0 37.0 33.0 37.0 6 35.12072920450363 37.0 37.0 37.0 33.0 37.0 7 35.00702916661396 37.0 37.0 37.0 33.0 37.0 8 35.004065459377635 37.0 37.0 37.0 33.0 37.0 9 34.945840960964 37.0 37.0 37.0 33.0 37.0 10-11 34.92391856310799 37.0 37.0 37.0 33.0 37.0 12-13 34.83217977655334 37.0 37.0 37.0 30.0 37.0 14-15 36.27196396204286 37.0 37.0 40.0 27.0 40.0 16-17 36.27072004833494 37.0 37.0 40.0 27.0 40.0 18-19 36.2961662157856 38.5 37.0 40.0 27.0 40.0 20-21 36.28598359238664 40.0 37.0 40.0 27.0 40.0 22-23 36.24131925933824 38.5 37.0 40.0 27.0 40.0 24-25 36.21179468135027 37.0 37.0 40.0 27.0 40.0 26-27 36.18865773578791 37.0 37.0 40.0 27.0 40.0 28-29 36.135464009932036 37.0 37.0 40.0 27.0 40.0 30-31 36.04361965636658 37.0 37.0 40.0 27.0 40.0 32-33 35.9992022989212 37.0 37.0 40.0 27.0 40.0 34-35 35.90399004146178 37.0 37.0 40.0 27.0 40.0 36-37 35.89140226723603 37.0 37.0 40.0 27.0 40.0 38-39 35.826619662992755 37.0 37.0 40.0 27.0 40.0 40-41 35.758375484840215 37.0 37.0 40.0 27.0 40.0 42-43 35.66683457995775 37.0 37.0 40.0 27.0 40.0 44-45 35.55697651864395 37.0 37.0 40.0 27.0 40.0 46-47 35.44950674153039 37.0 37.0 40.0 27.0 40.0 48-49 35.34030512329805 37.0 35.0 40.0 27.0 40.0 50-51 35.243096278920994 37.0 33.0 40.0 27.0 40.0 52-53 35.13713139644223 37.0 33.0 40.0 27.0 40.0 54-55 35.011722606640745 37.0 33.0 40.0 27.0 40.0 56-57 34.882478315843244 37.0 33.0 40.0 27.0 40.0 58-59 34.74769469357858 37.0 33.0 40.0 27.0 40.0 60-61 34.58063196237779 37.0 33.0 40.0 27.0 40.0 62-63 34.40977695091149 37.0 33.0 40.0 27.0 40.0 64-65 34.2136108556018 37.0 33.0 40.0 22.0 40.0 66-67 33.98500044877274 37.0 33.0 38.5 22.0 40.0 68-69 33.765313240362076 37.0 33.0 37.0 22.0 40.0 70-71 33.536647866401864 37.0 33.0 37.0 22.0 40.0 72-73 33.321156532534815 37.0 33.0 37.0 22.0 40.0 74-75 33.09107782562679 37.0 33.0 37.0 22.0 40.0 76-77 32.85974225385117 37.0 33.0 37.0 22.0 37.0 78-79 32.62344714089596 37.0 33.0 37.0 22.0 37.0 80-81 32.41598130936826 37.0 33.0 37.0 22.0 37.0 82-83 32.2122033958543 35.0 33.0 37.0 22.0 37.0 84-85 32.046677786592596 33.0 33.0 37.0 22.0 37.0 86-87 32.083700634245396 33.0 33.0 37.0 22.0 37.0 88-89 32.10101633472543 33.0 33.0 37.0 22.0 37.0 90-91 32.11782980128102 33.0 33.0 37.0 22.0 37.0 92-93 32.09557202862749 33.0 33.0 37.0 22.0 37.0 94-95 32.12201528477793 33.0 33.0 37.0 22.0 37.0 96-97 32.085645868308994 33.0 33.0 37.0 22.0 37.0 98-99 32.01029978621551 33.0 33.0 37.0 22.0 37.0 100 31.47057857950476 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7176557800521408 1101 2 4.233239943271457 1101 3 2.450318553268289 1101 4 1.2213125994197256 1101 5 0.5776200006251884 1101 6 0.5922593555773261 1101 7 0.6234790635036234 1101 8 0.5522670728689576 1101 9 0.6785821129445821 1101 10-11 0.6838825736189236 1101 12-13 0.7336329529482626 1101 14-15 1.0255788028573036 1101 16-17 1.0244718341432772 1101 18-19 1.0415688651601442 1101 20-21 1.0082521252492072 1101 22-23 1.007207109163467 1101 24-25 0.9711262195270862 1101 26-27 1.075698960093149 1101 28-29 0.8778729878527969 1101 30-31 0.8098827131475517 1101 32-33 0.9964757634980614 1101 34-35 1.130890362952094 1101 36-37 1.042251645719496 1101 38-39 1.070296641167829 1101 40-41 1.067430367772971 1101 42-43 1.114504746373406 1101 44-45 1.1007062623517072 1101 46-47 1.166843293619955 1101 48-49 1.1386503670582826 1101 50-51 1.1265376160673952 1101 52-53 1.0856880741597834 1101 54-55 1.0277687768637733 1101 56-57 1.0298207280705753 1101 58-59 1.095846245645376 1101 60-61 1.1029053013579855 1101 62-63 1.0766904535089168 1101 64-65 1.0520883797963165 1101 66-67 1.0739141169120856 1101 68-69 1.044340552111258 1101 70-71 0.9679508897927391 1101 72-73 0.9445582919837392 1101 74-75 0.9570916731406456 1101 76-77 0.9228363609478194 1101 78-79 0.9230377409168824 1101 80-81 0.9979066937389547 1101 82-83 0.9018376925834239 1101 84-85 1.038433457406537 1101 86-87 1.0230371600469148 1101 88-89 0.8879785654792265 1101 90-91 0.9219551740690086 1101 92-93 0.9214471516208391 1101 94-95 0.9355843594240163 1101 96-97 0.9223148379894042 1101 98-99 0.9063387312357527 1101 100 1.0963492616090704 1102 1 0.599375269915015 1102 2 4.116735915143483 1102 3 2.3426370603981077 1102 4 1.1049657204889058 1102 5 0.49405210035436653 1102 6 0.5326790257331169 1102 7 0.5413598774782642 1102 8 0.5548303626827931 1102 9 0.5847615056746207 1102 10-11 0.6344619138086003 1102 12-13 0.6919786725301478 1102 14-15 0.9131734841671104 1102 16-17 0.9232963809579857 1102 18-19 0.9302519600004331 1102 20-21 0.873230054163578 1102 22-23 0.8925167365377789 1102 24-25 0.9696419835067616 1102 26-27 0.8666373582764209 1102 28-29 0.8812281291110864 1102 30-31 0.9589402013992085 1102 32-33 1.012541791329916 1102 34-35 1.060755848194482 1102 36-37 0.9822101227015239 1102 38-39 1.0006955331008172 1102 40-41 1.011947085816601 1102 42-43 1.071137358803803 1102 44-45 1.0345019522714907 1102 46-47 1.023911251799234 1102 48-49 0.9442155073864953 1102 50-51 0.9757453025350884 1102 52-53 0.9557509063100156 1102 54-55 0.957841051336878 1102 56-57 1.0071080187079104 1102 58-59 0.9090551744411712 1102 60-61 0.9164249694884603 1102 62-63 1.0707338067880627 1102 64-65 0.9775239172625021 1102 66-67 0.9615201341218125 1102 68-69 0.944588575309254 1102 70-71 0.9258663760871215 1102 72-73 0.9004966518235449 1102 74-75 0.8724502149722824 1102 76-77 0.8413603618892935 1102 78-79 0.8550604980555008 1102 80-81 0.8376275985738246 1102 82-83 0.8847932084671548 1102 84-85 0.8717846876318838 1102 86-87 0.89149906424608 1102 88-89 0.8513694684047941 1102 90-91 0.9024170869200283 1102 92-93 0.9212934140084741 1102 94-95 0.9068461915768005 1102 96-97 0.9301245553233812 1102 98-99 0.9261118932911394 1102 100 0.9769752549750592 1103 1 0.5854343615999085 1103 2 4.070508435987325 1103 3 2.260417149434012 1103 4 1.0623356336840004 1103 5 0.477803617784474 1103 6 0.4812345683273094 1103 7 0.44971204091791606 1103 8 0.4139362739522241 1103 9 0.5184896826435406 1103 10-11 0.5292855837774724 1103 12-13 0.5147981402155679 1103 14-15 0.7827819626871744 1103 16-17 0.7378348270895501 1103 18-19 0.8459956163071922 1103 20-21 0.7304652669859664 1103 22-23 0.7072909783529653 1103 24-25 0.722139202760431 1103 26-27 0.7806509591723341 1103 28-29 0.7627886245251361 1103 30-31 0.6937920419941648 1103 32-33 0.784010553629173 1103 34-35 0.912380196227268 1103 36-37 0.7871917854827757 1103 38-39 0.680872418380801 1103 40-41 0.7556736240510418 1103 42-43 0.8276447254055128 1103 44-45 0.8800876938008457 1103 46-47 0.7632569565441045 1103 48-49 0.7495905464790198 1103 50-51 0.6691633525050804 1103 52-53 0.7361030001184545 1103 54-55 0.7568703862848025 1103 56-57 0.6922260686178774 1103 58-59 0.7330185848417159 1103 60-61 0.7158505520345528 1103 62-63 0.8180281641190206 1103 64-65 0.8035222297061395 1103 66-67 0.7735292065880941 1103 68-69 0.7149307126552102 1103 70-71 0.6942681037414573 1103 72-73 0.6572647052650993 1103 74-75 0.6063337615778721 1103 76-77 0.6880981597703055 1103 78-79 0.7583333149320239 1103 80-81 0.6515401141633177 1103 82-83 0.6644054706830751 1103 84-85 0.6587280356404612 1103 86-87 0.7244694615781562 1103 88-89 0.7804744632634915 1103 90-91 0.6772614121466027 1103 92-93 0.7346568234886064 1103 94-95 0.63417430003501 1103 96-97 0.5950455529144989 1103 98-99 0.6344774427303967 1103 100 0.7826237466290209 1104 1 0.4762004937173465 1104 2 3.6904066052903097 1104 3 2.197694569092061 1104 4 1.0668097023398317 1104 5 0.423418945126528 1104 6 0.38692705536389127 1104 7 0.4094750604170514 1104 8 0.33837557618777225 1104 9 0.47774447558867905 1104 10-11 0.4824667827855933 1104 12-13 0.4768094554421367 1104 14-15 0.6231433308342034 1104 16-17 0.6066558455607591 1104 18-19 0.6259640908933051 1104 20-21 0.6465356418702228 1104 22-23 0.5707448219418012 1104 24-25 0.6860832709259768 1104 26-27 0.6705711650421051 1104 28-29 0.5818353547798125 1104 30-31 0.6250090195662565 1104 32-33 0.7609016536191007 1104 34-35 0.8031596834701347 1104 36-37 0.6871802516956151 1104 38-39 0.6877239432921982 1104 40-41 0.6462982568867233 1104 42-43 0.6840254547035087 1104 44-45 0.7644091749651523 1104 46-47 0.6845991096537674 1104 48-49 0.588046156522033 1104 50-51 0.5536830464974827 1104 52-53 0.5678886499081202 1104 54-55 0.6289520677768081 1104 56-57 0.585048768086736 1104 58-59 0.5935834525763752 1104 60-61 0.6103546556966677 1104 62-63 0.5918791578338514 1104 64-65 0.5997993858393045 1104 66-67 0.6614111194713672 1104 68-69 0.6419611734806239 1104 70-71 0.615993957532389 1104 72-73 0.5433236740184952 1104 74-75 0.5926224384886467 1104 76-77 0.4911453481240855 1104 78-79 0.5341991234448074 1104 80-81 0.510095919764403 1104 82-83 0.5528123192727143 1104 84-85 0.6420092074179458 1104 86-87 0.6428974531404883 1104 88-89 0.6502983764429011 1104 90-91 0.5717786676597818 1104 92-93 0.6000318130504638 1104 94-95 0.5929639605904065 1104 96-97 0.5995980448182863 1104 98-99 0.5864654415748198 1104 100 0.5368008517728455 1105 1 0.5361848571643009 1105 2 3.8735946049123093 1105 3 2.2373538734458407 1105 4 1.0895531589405039 1105 5 0.3894450693215106 1105 6 0.3935749272819109 1105 7 0.36186509771067676 1105 8 0.31612058762922857 1105 9 0.3310857023611362 1105 10-11 0.4000998354413028 1105 12-13 0.3531683114646995 1105 14-15 0.48778040926684696 1105 16-17 0.5045589321400783 1105 18-19 0.5659411280370819 1105 20-21 0.5149181134336587 1105 22-23 0.5444182106726956 1105 24-25 0.6179017294791578 1105 26-27 0.5798457544339755 1105 28-29 0.5398068195054293 1105 30-31 0.5328710714682146 1105 32-33 0.49184109928402364 1105 34-35 0.6680824344293299 1105 36-37 0.5172635407342625 1105 38-39 0.49542954766813807 1105 40-41 0.47944105645699153 1105 42-43 0.5162929619339778 1105 44-45 0.5565087491417202 1105 46-47 0.4917477899454994 1105 48-49 0.43229868808366234 1105 50-51 0.42751446116891145 1105 52-53 0.38679995182744875 1105 54-55 0.43801935189735985 1105 56-57 0.463284297459289 1105 58-59 0.4712328161985937 1105 60-61 0.4107219343030266 1105 62-63 0.5001294382074164 1105 64-65 0.3906567478002927 1105 66-67 0.5012654810098738 1105 68-69 0.43979671621845284 1105 70-71 0.47320904397722785 1105 72-73 0.4605038754528792 1105 74-75 0.44510030327510464 1105 76-77 0.36032763511551025 1105 78-79 0.4394446458210979 1105 80-81 0.43518114902295935 1105 82-83 0.47071928834172994 1105 84-85 0.451592171856678 1105 86-87 0.38172981178577814 1105 88-89 0.39662343799593813 1105 90-91 0.43048860488603324 1105 92-93 0.40043932072781274 1105 94-95 0.4638639827907092 1105 96-97 0.42208307191679495 1105 98-99 0.42759430108015195 1105 100 0.3535945056445833 1106 1 0.4616534900614937 1106 2 3.8909785633682787 1106 3 2.2636189590833524 1106 4 1.0492821973430253 1106 5 0.33819822640560204 1106 6 0.2934491577977667 1106 7 0.25853729479535303 1106 8 0.22616916838478573 1106 9 0.19315539127058656 1106 10-11 0.2363982365909365 1106 12-13 0.24632711453258338 1106 14-15 0.32495279850213876 1106 16-17 0.2733819244381337 1106 18-19 0.29006806191168266 1106 20-21 0.2499412470788016 1106 22-23 0.26774861119842797 1106 24-25 0.23045285293054008 1106 26-27 0.2300586617565159 1106 28-29 0.2242679100624798 1106 30-31 0.20162914365840123 1106 32-33 0.3409362280491237 1106 34-35 0.48393389343394233 1106 36-37 0.2468746468185401 1106 38-39 0.20520406944281433 1106 40-41 0.2988286713166133 1106 42-43 0.38942938806945193 1106 44-45 0.30627475260828874 1106 46-47 0.2796835018086412 1106 48-49 0.15430654419257195 1106 50-51 0.1888884479446773 1106 52-53 0.12027461545302742 1106 54-55 0.17377920735011543 1106 56-57 0.17054961625701992 1106 58-59 0.1852261273014335 1106 60-61 0.21421031951837222 1106 62-63 0.38193168609763717 1106 64-65 0.21226645814758882 1106 66-67 0.1138105386681616 1106 68-69 0.13644111343076304 1106 70-71 0.08807864050928771 1106 72-73 0.12872824853503317 1106 74-75 0.1068357891521785 1106 76-77 0.20322001686226798 1106 78-79 0.21756730502448107 1106 80-81 0.1634115415482924 1106 82-83 0.16970929348657293 1106 84-85 0.15270866067737998 1106 86-87 0.29677586042538096 1106 88-89 0.24416547182613968 1106 90-91 0.22428260329764171 1106 92-93 0.18576866608766807 1106 94-95 0.2572642991389422 1106 96-97 0.2892414171281317 1106 98-99 0.288152075945721 1106 100 0.2876539741964699 1107 1 0.45094837019345846 1107 2 3.6834643235611217 1107 3 2.1253211279931925 1107 4 0.9490278963194356 1107 5 0.36864419026544937 1107 6 0.14110232609932183 1107 7 0.054398036711319264 1107 8 0.02275242248466469 1107 9 0.121241073266674 1107 10-11 0.07110137972896524 1107 12-13 0.0959817926454889 1107 14-15 0.10282553974276709 1107 16-17 0.05009964787849697 1107 18-19 0.13580401618273186 1107 20-21 0.13707331639279374 1107 22-23 0.13672293713511152 1107 24-25 0.1925535991117897 1107 26-27 0.11724025029777607 1107 28-29 0.04725739308335619 1107 30-31 0.08631663532778333 1107 32-33 0.12827461401312945 1107 34-35 0.26417624683319474 1107 36-37 0.12416071042771648 1107 38-39 0.03606208093483332 1107 40-41 0.03841180101255759 1107 42-43 0.17745407578730266 1107 44-45 0.2785101377717467 1107 46-47 0.11876836952443881 1107 48-49 0.09760597880093513 1107 50-51 0.0895078825853517 1107 52-53 0.07800058428903611 1107 54-55 0.0891311775771868 1107 56-57 0.054031884253788576 1107 58-59 0.08396033749399834 1107 60-61 0.11310001442984685 1107 62-63 0.19719887585322482 1107 64-65 0.036753837207179174 1107 66-67 0.0909048263465948 1107 68-69 0.0054736426353940715 1107 70-71 0.03975447859999548 1107 72-73 0.06355189788396842 1107 74-75 0.058047434975797785 1107 76-77 0.05441510242228986 1107 78-79 0.03904736040846757 1107 80-81 0.097374723639291 1107 82-83 0.1317218259709385 1107 84-85 0.14792541029346395 1107 86-87 0.12673422549921654 1107 88-89 0.03803041924124173 1107 90-91 0.04766593785616635 1107 92-93 0.189485374908962 1107 94-95 0.11411400788025716 1107 96-97 0.1288052803710542 1107 98-99 0.17105186696327124 1107 100 0.18311569454127863 1108 1 0.5150837181028187 1108 2 3.7263542548395385 1108 3 2.0776447025720195 1108 4 0.9330695835925553 1108 5 0.3085832303902265 1108 6 0.14301463181791263 1108 7 0.10519913830474081 1108 8 0.07366560149684886 1108 9 0.1322776715422691 1108 10-11 0.16065783070282436 1108 12-13 0.13229246856823096 1108 14-15 0.10321707923868217 1108 16-17 0.1427808867769187 1108 18-19 0.15106847906653798 1108 20-21 0.13491503365616353 1108 22-23 0.06116220462505595 1108 24-25 0.1027982188470844 1108 26-27 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0.11333003719951407 1108 92-93 0.1693059433503521 1108 94-95 0.13623925053517993 1108 96-97 0.06807109403563771 1108 98-99 0.06657039570129442 1108 100 0.12769517868443714 1109 1 0.562582177372267 1109 2 3.932247863293359 1109 3 2.255930076292607 1109 4 1.1291241434408477 1109 5 0.4627961877361315 1109 6 0.40189363668456224 1109 7 0.25989038555256627 1109 8 0.19625660013767998 1109 9 0.18900270873447056 1109 10-11 0.2361021863172894 1109 12-13 0.24564169921064405 1109 14-15 0.29040757344090906 1109 16-17 0.15769473364212416 1109 18-19 0.22284635818738963 1109 20-21 0.2501426351103433 1109 22-23 0.1336802983749621 1109 24-25 0.16812158609595684 1109 26-27 0.18643194097158755 1109 28-29 0.1479132097712892 1109 30-31 0.1593963620539327 1109 32-33 0.3048541792059538 1109 34-35 0.40659067191041487 1109 36-37 0.2610195751568227 1109 38-39 0.1907731066520597 1109 40-41 0.12684695282153768 1109 42-43 0.2125236839277207 1109 44-45 0.22514271781486173 1109 46-47 0.14211141282960682 1109 48-49 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90-91 1.139827062259453 2202 92-93 1.2130501366217956 2202 94-95 1.1912894133994136 2202 96-97 1.1704236768396896 2202 98-99 1.1574245070850857 2202 100 1.0414300596334343 2203 1 0.4563396651545695 2203 2 4.013320560685752 2203 3 2.271441783855419 2203 4 1.0575520644854777 2203 5 0.5504868930122342 2203 6 0.591310242729385 2203 7 0.5636560491347069 2203 8 0.6030111176546527 2203 9 0.6165516914537363 2203 10-11 0.6158637421504025 2203 12-13 0.7101737523882576 2203 14-15 0.9919357511184401 2203 16-17 1.034885469126536 2203 18-19 1.0360108592498989 2203 20-21 1.0358279440398164 2203 22-23 1.0092077664110306 2203 24-25 1.086988544549186 2203 26-27 1.0123332602411352 2203 28-29 1.0307927400923802 2203 30-31 1.0287333022242606 2203 32-33 1.0750937752000311 2203 34-35 1.2745100677676362 2203 36-37 1.1077072461289106 2203 38-39 1.091309351031832 2203 40-41 0.7069061871650533 2203 42-43 0.898469305803566 2203 44-45 1.07463171945885 2203 46-47 1.1538559210406873 2203 48-49 1.0688780070311097 2203 50-51 0.9753716988670291 2203 52-53 0.9959171867717416 2203 54-55 1.101029287988304 2203 56-57 0.9828017151272519 2203 58-59 1.0585421207272603 2203 60-61 1.0284219040423679 2203 62-63 1.139838686244282 2203 64-65 1.0172607519371297 2203 66-67 1.0098564222520068 2203 68-69 0.9597872281896969 2203 70-71 1.0198663320344323 2203 72-73 1.0270009225570007 2203 74-75 0.9964854974882869 2203 76-77 0.9062134680064986 2203 78-79 0.9193878925883681 2203 80-81 0.9659625787079094 2203 82-83 0.9417787858693281 2203 84-85 0.9890744653414352 2203 86-87 0.9920660825030048 2203 88-89 0.9540251274626108 2203 90-91 0.9752057414315836 2203 92-93 1.0415592127919098 2203 94-95 1.0277013842777158 2203 96-97 1.024424680854132 2203 98-99 1.066805088538377 2203 100 0.8312690481744696 2204 1 0.35009925175615564 2204 2 3.865540477706759 2204 3 2.264223943008517 2204 4 1.039521871922176 2204 5 0.5623151035477818 2204 6 0.5066961829376524 2204 7 0.46148390366488456 2204 8 0.4241916375014654 2204 9 0.41047668244702606 2204 10-11 0.5106788408562508 2204 12-13 0.5236988406396534 2204 14-15 0.621156033947635 2204 16-17 0.6792124847102841 2204 18-19 0.736493379910911 2204 20-21 0.6874182490090348 2204 22-23 0.5118121434932874 2204 24-25 0.6522230115657166 2204 26-27 0.565895625134381 2204 28-29 0.6427880071579892 2204 30-31 0.6975593589096647 2204 32-33 0.7834901564708971 2204 34-35 0.8191950767847516 2204 36-37 0.6161960646391833 2204 38-39 0.5140325830655144 2204 40-41 0.5295152271671739 2204 42-43 0.6551592923737104 2204 44-45 0.7467904201370956 2204 46-47 0.6588343777261869 2204 48-49 0.4626994162179088 2204 50-51 0.47169356936582574 2204 52-53 0.5498630402845919 2204 54-55 0.5461248700858619 2204 56-57 0.48207105153556284 2204 58-59 0.5925420280425016 2204 60-61 0.5215101802701554 2204 62-63 0.6372139864850723 2204 64-65 0.518669980774149 2204 66-67 0.44424085347449704 2204 68-69 0.5133100773123118 2204 70-71 0.5056141634260314 2204 72-73 0.44154306403910226 2204 74-75 0.39562195953948276 2204 76-77 0.45518954789157107 2204 78-79 0.5254006605729202 2204 80-81 0.512183153087463 2204 82-83 0.5524720068195386 2204 84-85 0.6627592527048414 2204 86-87 0.6091430305229402 2204 88-89 0.5897835759549928 2204 90-91 0.5226008958178276 2204 92-93 0.7170639258358023 2204 94-95 0.6643356625497141 2204 96-97 0.746765797448603 2204 98-99 0.8238286656135934 2204 100 0.5532779690536849 2205 1 0.5143406668453387 2205 2 3.841742391653849 2205 3 2.259135125101956 2205 4 1.0918906988899053 2205 5 0.5537227745878042 2205 6 0.47643484023565463 2205 7 0.40954253676532204 2205 8 0.40269451803736445 2205 9 0.4823428002365233 2205 10-11 0.5535925683126095 2205 12-13 0.5472278363265204 2205 14-15 0.7007872059464262 2205 16-17 0.7732556076562744 2205 18-19 0.7904810817090606 2205 20-21 0.7946064944692921 2205 22-23 0.7160445268290161 2205 24-25 0.7285792487731442 2205 26-27 0.7379842554855855 2205 28-29 0.7476060433812393 2205 30-31 0.7481563968864506 2205 32-33 0.691202614886798 2205 34-35 0.9168184361697413 2205 36-37 0.7496037324595477 2205 38-39 0.7406762120432901 2205 40-41 0.7047856605504279 2205 42-43 0.7684329212137513 2205 44-45 0.7397607510734332 2205 46-47 0.7822322595570128 2205 48-49 0.6992444060224088 2205 50-51 0.6883524839960558 2205 52-53 0.7299992801635469 2205 54-55 0.6898388410283118 2205 56-57 0.5990044047513123 2205 58-59 0.6346900319770938 2205 60-61 0.5801455364606767 2205 62-63 0.7108062416113938 2205 64-65 0.6106503425733436 2205 66-67 0.5567536777237194 2205 68-69 0.5076045745231355 2205 70-71 0.5851810017703443 2205 72-73 0.5984888698018267 2205 74-75 0.5812808570975463 2205 76-77 0.5978458691819171 2205 78-79 0.5765844624200156 2205 80-81 0.5933666800706661 2205 82-83 0.6712454973233513 2205 84-85 0.6733869061078686 2205 86-87 0.670157718331513 2205 88-89 0.6681491815496194 2205 90-91 0.6077254007174879 2205 92-93 0.6648985140530144 2205 94-95 0.6189846395879606 2205 96-97 0.6028158867535325 2205 98-99 0.7058457119054573 2205 100 0.5256422423831957 2206 1 0.4401046319109341 2206 2 3.6936217061457732 2206 3 2.1988494133471264 2206 4 0.9664829463373863 2206 5 0.5022374217515946 2206 6 0.36818480307542956 2206 7 0.30199117997177893 2206 8 0.24475570688615989 2206 9 0.33292686552326245 2206 10-11 0.2677124608745416 2206 12-13 0.32421365976157546 2206 14-15 0.4626369372977095 2206 16-17 0.4481219890271575 2206 18-19 0.35314482396904623 2206 20-21 0.3241646961421907 2206 22-23 0.3972005889685022 2206 24-25 0.2763761171128323 2206 26-27 0.34161820911643304 2206 28-29 0.3624030356370156 2206 30-31 0.49732168624803563 2206 32-33 0.25964245294512267 2206 34-35 0.43101481990311186 2206 36-37 0.2497446541557764 2206 38-39 0.41150361036712013 2206 40-41 0.41867716982598324 2206 42-43 0.4559646558511119 2206 44-45 0.5424154074515926 2206 46-47 0.588943527578742 2206 48-49 0.4424527969617955 2206 50-51 0.28053883977383975 2206 52-53 0.19263562071220264 2206 54-55 0.23065680011965384 2206 56-57 0.2813134705515381 2206 58-59 0.2306616494499778 2206 60-61 0.3102334818394752 2206 62-63 0.38404171984816315 2206 64-65 0.10905047199897666 2206 66-67 0.22396298912698853 2206 68-69 0.0033027394650844144 2206 70-71 0.22606120119639428 2206 72-73 0.2731348109756553 2206 74-75 0.2122507871506656 2206 76-77 0.22466560320328455 2206 78-79 0.1820241450235187 2206 80-81 0.24559241449249924 2206 82-83 0.16498421115223394 2206 84-85 0.20686718334893683 2206 86-87 0.2602031389799002 2206 88-89 0.23844187173672182 2206 90-91 0.18507533108403962 2206 92-93 0.29464301816040006 2206 94-95 0.2810155921327713 2206 96-97 0.24087801241963547 2206 98-99 0.23006622569289803 2206 100 0.09739030900507828 2207 1 0.3973131093114546 2207 2 3.868508593103595 2207 3 2.210431086524501 2207 4 0.9820485017059326 2207 5 0.44403358894925304 2207 6 0.31363828376820635 2207 7 0.2912491564466251 2207 8 0.1752544475593183 2207 9 0.24390599091363896 2207 10-11 0.16605914533614907 2207 12-13 0.22387734715324115 2207 14-15 0.25562638926662373 2207 16-17 0.28769974298648293 2207 18-19 0.4133725362460865 2207 20-21 0.28644665149932536 2207 22-23 0.28337269795465403 2207 24-25 0.2711782812455965 2207 26-27 0.2610078563572884 2207 28-29 0.25817929572663445 2207 30-31 0.1969755805157618 2207 32-33 0.32278172494692114 2207 34-35 0.4529909837346864 2207 36-37 0.3769085654605817 2207 38-39 0.29872786503165116 2207 40-41 0.24985065181795107 2207 42-43 0.3300386150979264 2207 44-45 0.3593871865226532 2207 46-47 0.2692966016846441 2207 48-49 0.18012182677324517 2207 50-51 0.20325642674809785 2207 52-53 0.21856978139226868 2207 54-55 0.28190156030622404 2207 56-57 0.22696842385457217 2207 58-59 0.19004311770992643 2207 60-61 0.2593962435594861 2207 62-63 0.3704281949042354 2207 64-65 0.3416915938677576 2207 66-67 0.3130001035903973 2207 68-69 0.09515190764008707 2207 70-71 0.10993177841985613 2207 72-73 0.24799428702876725 2207 74-75 0.2545455670711263 2207 76-77 0.22231535187924578 2207 78-79 0.20138933406033743 2207 80-81 0.17228586215440345 2207 82-83 0.26085771351784715 2207 84-85 -0.1461498862137347 2207 86-87 0.1330655559228937 2207 88-89 0.179207743241097 2207 90-91 0.3158543322641343 2207 92-93 0.44549541363726064 2207 94-95 0.36735019808009994 2207 96-97 0.3551265156464396 2207 98-99 0.38916590788388206 2207 100 0.276598178149257 2208 1 0.4812208166085945 2208 2 3.837170443200634 2208 3 2.199487141023237 2208 4 1.0541522740306988 2208 5 0.5340109204282442 2208 6 0.4653411729242478 2208 7 0.3434741680362521 2208 8 0.2814568613442958 2208 9 0.3003413758070508 2208 10-11 0.34835422504919933 2208 12-13 0.3191293331042644 2208 14-15 0.4103132140236383 2208 16-17 0.3493751116937105 2208 18-19 0.4580399336484362 2208 20-21 0.4105778224836172 2208 22-23 0.05186836461346189 2208 24-25 0.34314327272034717 2208 26-27 0.30293941471006747 2208 28-29 0.3112376799364611 2208 30-31 0.3661042567841406 2208 32-33 0.45359176116941313 2208 34-35 0.40773565840016346 2208 36-37 0.3682321595801312 2208 38-39 0.31522481244225276 2208 40-41 0.3983972342535793 2208 42-43 0.3675568834900744 2208 44-45 0.010102796765522726 2208 46-47 0.09389302736568084 2208 48-49 0.13826324377188115 2208 50-51 0.20302355385682347 2208 52-53 0.2907965216039443 2208 54-55 0.3368509195374614 2208 56-57 0.2539826614143834 2208 58-59 0.30011283225156404 2208 60-61 0.18151956109949907 2208 62-63 0.3462898540671091 2208 64-65 0.26896198178626207 2208 66-67 0.31781451244094683 2208 68-69 0.27252375388701466 2208 70-71 0.27490478834371856 2208 72-73 0.24800008184132594 2208 74-75 0.3036784077471637 2208 76-77 0.2233813027392344 2208 78-79 0.07910994944978711 2208 80-81 0.05756625250868552 2208 82-83 0.26670045605530035 2208 84-85 0.25215557308900216 2208 86-87 0.4368361127749161 2208 88-89 0.30441282067550546 2208 90-91 0.4029920091532482 2208 92-93 0.3440907117852703 2208 94-95 0.31411580871584377 2208 96-97 0.18156195947015163 2208 98-99 0.22393900659605848 2208 100 -0.2372765674682924 2209 1 0.5561693205198281 2209 2 3.750343791233899 2209 3 2.1707539448069255 2209 4 1.0712169352065075 2209 5 0.5040832002457947 2209 6 0.41856442661235604 2209 7 0.278770760801784 2209 8 0.29058216900833145 2209 9 0.2631424387727961 2209 10-11 0.3300899057394773 2209 12-13 0.2159517850901409 2209 14-15 0.27333303885972526 2209 16-17 0.2573903196390859 2209 18-19 0.3263248282320035 2209 20-21 0.3239512872516457 2209 22-23 0.43729489428663726 2209 24-25 0.3821095601534452 2209 26-27 0.39193094835160025 2209 28-29 0.3112946962206564 2209 30-31 0.26550826869232225 2209 32-33 0.41982866325118096 2209 34-35 0.5484759359077245 2209 36-37 0.2696932356399273 2209 38-39 0.21759432737534468 2209 40-41 0.24099986356512204 2209 42-43 0.37382933099804205 2209 44-45 0.28579015760255544 2209 46-47 0.20826263648952192 2209 48-49 0.12611093887797864 2209 50-51 0.15479847039765104 2209 52-53 0.2546700465922882 2209 54-55 0.24697650205317956 2209 56-57 0.31447026066209816 2209 58-59 0.3091406679334838 2209 60-61 -0.1631251158880005 2209 62-63 0.19376527182622993 2209 64-65 0.27477687071838375 2209 66-67 0.28177944877725736 2209 68-69 0.2797228993762104 2209 70-71 0.24751898304371167 2209 72-73 0.3243851876592245 2209 74-75 0.2569769754301703 2209 76-77 0.29624475549407947 2209 78-79 0.289986432954052 2209 80-81 0.29395406824070136 2209 82-83 -0.02378272703606754 2209 84-85 0.06467170904485187 2209 86-87 0.2727881804934249 2209 88-89 0.3440944559504082 2209 90-91 0.3143068656590522 2209 92-93 0.3621724014128631 2209 94-95 0.3282541872761833 2209 96-97 0.33336034649220636 2209 98-99 0.3293314654686732 2209 100 0.16336091859610846 2210 1 0.43800926869985446 2210 2 3.9214392473078377 2210 3 2.2158583738963387 2210 4 0.9800599963936705 2210 5 0.49675662458756165 2210 6 0.41255572020991593 2210 7 0.30310976527081124 2210 8 0.2789033704052599 2210 9 0.2397900590427895 2210 10-11 0.2574111152234906 2210 12-13 0.28373521339458563 2210 14-15 0.22462773599958297 2210 16-17 0.22477535450878605 2210 18-19 0.22790649960352738 2210 20-21 0.3011950367804417 2210 22-23 0.19141732001651945 2210 24-25 0.2741094996307254 2210 26-27 0.20086892847353965 2210 28-29 0.2977397258050445 2210 30-31 0.19132550806830295 2210 32-33 0.3750950988880035 2210 34-35 0.37235918079178987 2210 36-37 0.24647807553253642 2210 38-39 0.14281481791724104 2210 40-41 0.14837691178320966 2210 42-43 0.2357583141041104 2210 44-45 0.3836071068868563 2210 46-47 0.29880694577524736 2210 48-49 0.1517317467989443 2210 50-51 0.17064365196712572 2210 52-53 0.2570721400002398 2210 54-55 0.0970065742813162 2210 56-57 0.11793044518857698 2210 58-59 0.12887223661203961 2210 60-61 0.2526826237682869 2210 62-63 0.18873520090247808 2210 64-65 -0.17377718403015763 2210 66-67 0.03617207455300786 2210 68-69 0.13915297228260926 2210 70-71 0.18555020363833563 2210 72-73 0.21423546174629138 2210 74-75 -0.44743092450698896 2210 76-77 -0.02510498080162904 2210 78-79 0.15705035825439495 2210 80-81 0.16623263268345312 2210 82-83 0.15990119460682806 2210 84-85 0.2053495074577505 2210 86-87 0.2209092499403802 2210 88-89 0.2724319971782556 2210 90-91 0.2312309495550231 2210 92-93 0.31040157044370176 2210 94-95 0.2124238037734223 2210 96-97 0.1553336364057074 2210 98-99 0.11888443472130561 2210 100 0.10902357850196864 2211 1 0.5081315616786952 2211 2 3.9299355379189294 2211 3 2.1540552080184234 2211 4 0.9867059469191943 2211 5 0.500584792403906 2211 6 0.2587542952546329 2211 7 0.3310413773388632 2211 8 0.17523306946468153 2211 9 0.2247062196838172 2211 10-11 0.20655426323664727 2211 12-13 0.06225830664267562 2211 14-15 0.08898541624387946 2211 16-17 0.07177240209279745 2211 18-19 0.32802731226907866 2211 20-21 0.17720613902707782 2211 22-23 0.17365122442647873 2211 24-25 0.1958334596436373 2211 26-27 0.13722090197094872 2211 28-29 0.17678852780766618 2211 30-31 0.21683971596687712 2211 32-33 0.17068849925352225 2211 34-35 0.3491307325850812 2211 36-37 0.2732242689304414 2211 38-39 0.07614642324503507 2211 40-41 0.19360376369756693 2211 42-43 0.24593484103490226 2211 44-45 0.30394818151323477 2211 46-47 0.27616079764559487 2211 48-49 0.2751865431187994 2211 50-51 0.11716607173965343 2211 52-53 0.20078818258723885 2211 54-55 0.19662708983640442 2211 56-57 0.0541231192489775 2211 58-59 0.08936408676421337 2211 60-61 0.06473201325843547 2211 62-63 0.15645810150714823 2211 64-65 0.05420140367949955 2211 66-67 0.059724171669799375 2211 68-69 0.10608004560497619 2211 70-71 0.15048086372305391 2211 72-73 0.16335566129622947 2211 74-75 0.1944019836785813 2211 76-77 0.040853815095253765 2211 78-79 0.11991790683600811 2211 80-81 0.2020595123627018 2211 82-83 -0.3348310681119848 2211 84-85 0.06845766449243129 2211 86-87 0.1391591115024049 2211 88-89 0.194746485048082 2211 90-91 0.13923937232810246 2211 92-93 0.3436388981253913 2211 94-95 0.29064338298311654 2211 96-97 0.2217215994832813 2211 98-99 0.2410759147058279 2211 100 0.00590407503250745 2212 1 0.6111913679056364 2212 2 4.028172348704942 2212 3 2.330389873689647 2212 4 1.196533504813253 2212 5 0.6401052861984624 2212 6 0.5313679799053261 2212 7 0.48498709515071425 2212 8 0.40995172947473435 2212 9 0.29390272005372964 2212 10-11 0.30354401760907024 2212 12-13 0.3974888671218437 2212 14-15 0.4761207797418834 2212 16-17 0.4800001387605306 2212 18-19 0.43489888302265456 2212 20-21 0.45788304962839277 2212 22-23 0.41889014799226487 2212 24-25 0.48358056430581087 2212 26-27 0.26130468689403585 2212 28-29 0.22183758899193862 2212 30-31 0.2956206267984314 2212 32-33 0.4948951910771697 2212 34-35 0.4074671555202585 2212 36-37 0.31766429764257964 2212 38-39 0.21430778849352805 2212 40-41 0.3878065279177747 2212 42-43 0.4203290674578568 2212 44-45 0.44139745702276656 2212 46-47 0.5247605731627445 2212 48-49 0.33934483400184234 2212 50-51 0.349482672128012 2212 52-53 0.32097386342543643 2212 54-55 0.21386685648258918 2212 56-57 0.303042901334706 2212 58-59 0.2233053098402351 2212 60-61 0.1895870982351795 2212 62-63 0.26963174051638106 2212 64-65 0.4630126941850321 2212 66-67 0.42862500137602666 2212 68-69 0.316311918106841 2212 70-71 0.33879548168744833 2212 72-73 0.4535915460481661 2212 74-75 0.3998257162223311 2212 76-77 0.35968819720027767 2212 78-79 0.46850083767463246 2212 80-81 0.49249977639245657 2212 82-83 0.4237399952697096 2212 84-85 0.5468120409769277 2212 86-87 0.42223410333400935 2212 88-89 0.5493112659572184 2212 90-91 0.29389214340468683 2212 92-93 0.4350785425418451 2212 94-95 0.3769763387924989 2212 96-97 0.39110842779114563 2212 98-99 0.4183248062582656 2212 100 0.3059361561036624 2213 1 -0.6401369136029409 2213 2 -25.719114473000484 2213 3 -14.645708926291249 2213 4 -6.328733345607219 2213 5 -2.234148389580035 2213 6 -1.4643709829252742 2213 7 -1.580401021541384 2213 8 -1.4520002753804135 2213 9 -1.1354820137701793 2213 10-11 -1.287310086366361 2213 12-13 -1.2239324292134768 2213 14-15 -1.645152164898775 2213 16-17 -1.6323845585373888 2213 18-19 -1.575134734932405 2213 20-21 -1.5353998918935048 2213 22-23 -1.6379862199761277 2213 24-25 -1.7011696260507705 2213 26-27 -1.6695071593259456 2213 28-29 -1.775157657010574 2213 30-31 -1.7309454950691148 2213 32-33 -1.6629630234764718 2213 34-35 -1.455146812578512 2213 36-37 -1.7322943296546995 2213 38-39 -1.655205025103129 2213 40-41 -1.5821025926207852 2213 42-43 -1.5955403493345415 2213 44-45 -1.557126190743105 2213 46-47 -1.5267119859882783 2213 48-49 -1.6820192851178248 2213 50-51 -1.6161402645050202 2213 52-53 -1.6974664208029182 2213 54-55 -1.6526738657766273 2213 56-57 -1.612037483930152 2213 58-59 -1.7100723463715681 2213 60-61 -1.7744692950766279 2213 62-63 -1.6320140360963151 2213 64-65 -1.5972120530130098 2213 66-67 -1.4513714022192516 2213 68-69 -1.623159115381867 2213 70-71 -1.6796845596712089 2213 72-73 -1.643912394543733 2213 74-75 -1.748787139238722 2213 76-77 -1.5222025001931705 2213 78-79 -1.5507365559434376 2213 80-81 -1.7341958019965276 2213 82-83 -1.7495873253244802 2213 84-85 -1.5650603793753568 2213 86-87 -1.5642143346181783 2213 88-89 -1.5801964620983888 2213 90-91 -1.4938918812531625 2213 92-93 -1.518332773770382 2213 94-95 -1.7056766278199404 2213 96-97 -1.8121341665653112 2213 98-99 -1.652772532683212 2213 100 -1.8831033790610299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 19.0 4 34.0 5 63.0 6 55.0 7 57.0 8 66.0 9 100.0 10 511.0 11 2491.0 12 6996.0 13 12996.0 14 16774.0 15 19140.0 16 19865.0 17 20441.0 18 21281.0 19 22479.0 20 24474.0 21 26892.0 22 29390.0 23 31724.0 24 33141.0 25 34953.0 26 38051.0 27 43182.0 28 50824.0 29 60075.0 30 72678.0 31 88764.0 32 111769.0 33 148748.0 34 203181.0 35 287944.0 36 438281.0 37 739862.0 38 708179.0 39 4682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.353545114768025 20.68828915642154 10.737312055343594 30.220853673466845 2 12.851276602273948 22.58856556140324 36.451764278663454 28.108393557659355 3 15.426638306638885 27.16695490526678 28.53917545086601 28.867231337228322 4 10.684459641405535 18.549489421866877 37.28464361941492 33.481407317312666 5 11.858432495507987 38.65270371536866 34.758529395083585 14.73033439403977 6 29.94331672926468 39.431443499204256 17.664723983602265 12.960515787928806 7 26.14363330456875 32.98563739267515 23.199762812694342 17.670966490061755 8 24.44036118929594 36.64708773276789 20.756632937626538 18.15591814030963 9 24.729520679246146 16.95822327643821 21.779332987418712 36.53292305689693 10-11 23.20873032146905 27.620566148685633 28.82019738833421 20.350506141511108 12-13 24.080626370154583 25.13870849498477 29.157883841776723 21.62278129308393 14-15 21.65709850167719 26.547198192996902 26.33518388835922 25.460519416966687 16-17 21.01536881340745 30.050755334161 27.116674899663646 21.817200952767905 18-19 20.94414050272252 29.07380074578953 29.059489926237518 20.92256882525043 20-21 22.220264014590757 28.281142581014063 28.243484741947398 21.255108662447782 22-23 21.4530803258531 28.264100620950167 28.539154596150418 21.743664457046314 24-25 21.62075816434275 28.0903398589833 28.367944834483872 21.92095714219008 26-27 21.47051337083644 28.104602037819397 28.46190573014331 21.962978861200853 28-29 21.18846005655618 28.079689570605005 28.828153736175384 21.903696636663433 30-31 21.87101064731166 28.3408887044503 28.147659993989848 21.64044065424819 32-33 21.024345424647763 28.368995549997507 28.85212706022887 21.75453196512586 34-35 21.302121618880278 27.86687070062956 28.804691998022307 22.026315682467853 36-37 21.75075756583153 28.286960711094565 28.225274122039146 21.737007601034755 38-39 21.498890114313934 28.28563136674451 28.530711355473215 21.684767163468337 40-41 21.685023733184696 28.06547548675356 28.303673315954452 21.945827464107296 42-43 21.302353068926656 28.227128028161104 28.491533702009264 21.97898520090298 44-45 21.685318936034474 27.777493296448487 28.39701832775984 22.140169439757194 46-47 21.77898595583382 28.343965641633222 28.13984460171244 21.73720380082052 48-49 21.563037392581737 28.157985435342976 28.33513292689834 21.94384424517695 50-51 21.77454279031928 28.085062924747128 28.01988551249374 22.12050877243985 52-53 21.451890420022035 28.304977459814662 28.016578176015 22.226553944148304 54-55 21.700616001703967 28.157185727511358 27.870996644181062 22.27120162660361 56-57 21.832777445074907 28.023064141230964 27.919061022794228 22.2250973908999 58-59 21.50066686096382 27.710599662766416 28.36431167974407 22.424421796525696 60-61 21.927417634917518 28.072326320317426 27.85575763344752 22.14449841131754 62-63 21.34717849522356 28.351739090224708 28.15767506355582 22.14340735099591 64-65 21.377850922381164 27.824231228528085 28.228635328963936 22.569282520126816 66-67 21.596385637445778 28.024293977553633 27.953769683199507 22.425550701801082 68-69 21.738810817585506 27.944876879525953 28.025107345531495 22.291204957357042 70-71 21.886892954670895 27.871286144338743 27.73867856996522 22.503142331025142 72-73 21.497797648806895 28.07836968921248 28.17789388012841 22.245938781852225 74-75 21.80761732004183 27.759357532368995 27.934712644924804 22.49831250266437 76-77 21.66076189147816 28.067125402000944 27.905323365331736 22.36678934118916 78-79 21.655452794571435 27.97460314400112 28.110100437594802 22.259843623832644 80-81 21.821207578509533 27.82542214580246 27.834127813357718 22.51924246233029 82-83 21.312577817746035 28.06454134388038 28.131200851603072 22.491679986770514 84-85 21.466360179662995 27.656531128201312 27.96982080992936 22.907287882206333 86-87 21.517444649356186 27.970643318274956 27.865262289119336 22.646649743249522 88-89 21.320901884943687 27.68706172615642 28.153881117685746 22.838155271214145 90-91 21.577554330080616 27.653474872074153 27.9260741012057 22.842896696639528 92-93 21.133991973249586 28.041819583505962 28.118686509297298 22.705501933947154 94-95 21.087888818124824 27.484259178801178 28.29075366339574 23.137098339678257 96-97 21.2456795762457 27.783794105745514 28.126416077194534 22.84411024081426 98-99 21.20102272888137 27.627203335284058 28.112725468858663 23.059048466975913 100 21.318426314251692 27.33838308152436 28.04295773607412 23.30023286814983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 43.0 1 38.0 2 31.0 3 30.5 4 29.0 5 41.5 6 73.5 7 142.0 8 213.0 9 236.0 10 229.5 11 217.5 12 248.5 13 292.0 14 363.0 15 462.5 16 541.5 17 659.5 18 832.0 19 1068.5 20 1374.0 21 1862.5 22 2699.0 23 3951.0 24 5679.5 25 8260.5 26 12394.0 27 18218.0 28 26155.5 29 35963.0 30 46949.0 31 58779.5 32 70486.0 33 82300.5 34 93650.0 35 103422.0 36 112507.5 37 121002.5 38 128344.5 39 133383.5 40 135956.5 41 136788.5 42 135303.0 43 132741.0 44 129101.5 45 125154.5 46 121256.5 47 116789.0 48 114112.0 49 111488.0 50 113123.5 51 107011.0 52 91332.0 53 82464.0 54 76694.0 55 71101.0 56 65920.5 57 60531.0 58 54688.5 59 49119.5 60 42996.0 61 36341.5 62 30177.0 63 24354.0 64 19570.0 65 15715.0 66 12027.5 67 9099.5 68 7036.0 69 5382.5 70 3961.5 71 2942.5 72 2187.5 73 1556.0 74 1089.5 75 734.0 76 479.0 77 310.0 78 179.0 79 89.5 80 45.0 81 23.0 82 14.5 83 8.5 84 3.5 85 4.0 86 4.5 87 1.5 88 2.0 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8565535578642754 2 5.778686969265995 3 0.0 4 0.006144271099698027 5 0.1624617564302508 6 0.05731641128786934 7 0.03825109949321811 8 0.044064061857148104 9 0.04300989769788619 10-11 0.04456102496080015 12-13 0.04293460025793891 14-15 0.045841081439903904 16-17 0.03627830656659938 18-19 0.030088857002932987 20-21 0.024215656687045166 22-23 0.024622262862760477 24-25 0.02111340216121724 26-27 0.013282468406700148 28-29 0.019125549746609054 30-31 0.023598217679477472 32-33 0.0016716031668296103 34-35 0.003930526365248003 36-37 0.004743738716678624 38-39 0.00691230498716028 40-41 0.007123137819012663 42-43 0.008056826074358932 44-45 0.008553789178010978 46-47 0.017077459380043045 48-49 0.027046840429062885 50-51 0.023025957135878145 52-53 0.029606953387270395 54-55 0.025811962413927494 56-57 0.01668591269231719 58-59 0.015315499285276701 60-61 0.013071635574847765 62-63 0.01936650155444035 64-65 0.021504948848943096 66-67 0.022363339664342084 68-69 0.029456358507375834 70-71 0.029335882603460192 72-73 0.026278806541600627 74-75 0.027152256844989075 76-77 0.02040560622571281 78-79 0.016339544468559702 80-81 0.014743238741677373 82-83 0.009321823065473233 84-85 0.0118969955116702 86-87 0.00929170408949432 88-89 0.007605041434675254 90-91 0.008252599418221861 92-93 0.004291954076994946 94-95 0.001701722142808522 96-97 0.0010391046712724605 98-99 8.734503033884451E-4 100 0.11134985419403728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3320166.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.09067789448491 #Duplication Level Percentage of deduplicated Percentage of total 1 89.37777777132841 74.26460143722302 2 7.8770857146128925 13.090247837202968 3 1.5126426321965931 3.770595051639388 4 0.5045677627342994 1.6769950979718622 5 0.2357010042795072 0.9792278112997571 6 0.1278434299893756 0.6373558357304003 7 0.08211914721847112 0.47763349273498396 8 0.05194606177919173 0.3452986789745467 9 0.03497340140847481 0.26153672681754947 >10 0.18234905011406505 2.611325867730554 >50 0.008952400596811282 0.4882098732663474 >100 0.0033144858157027075 0.5631381885136845 >500 2.908551752137017E-4 0.16515365206686922 >1k 3.9992585500998125E-4 0.5174834465066583 >5k 3.635689590999823E-5 0.15119700232141398 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5005 0.15074547477445405 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3821 0.11508460721542237 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.0118975978911897E-5 0.0 4 0.0 0.0 0.0 9.03569279367357E-5 0.0 5 0.0 0.0 0.0 9.03569279367357E-5 0.0 6 0.0 0.0 0.0 9.03569279367357E-5 0.0 7 0.0 0.0 0.0 9.03569279367357E-5 0.0 8 0.0 0.0 0.0 9.03569279367357E-5 0.0 9 0.0 0.0 0.0 1.807138558734714E-4 0.0 10-11 0.0 0.0 0.0 2.108328318523833E-4 0.0 12-13 0.0 1.5059487989455948E-5 0.0 2.4095180783129518E-4 0.0 14-15 0.0 3.0118975978911897E-5 0.0 2.710707838102071E-4 0.0 16-17 0.0 3.0118975978911897E-5 0.0 2.710707838102071E-4 0.0 18-19 0.0 4.517846396836784E-5 0.0 3.162492477785749E-4 0.0 20-21 0.0 6.0237951957823794E-5 0.0 3.463682237574868E-4 0.0 22-23 0.0 6.0237951957823794E-5 0.0 4.066061757153106E-4 0.0 24-25 0.0 6.0237951957823794E-5 0.0 5.87320031588782E-4 0.0 26-27 0.0 6.0237951957823794E-5 0.0 7.830933754517093E-4 0.0 28-29 0.0 6.0237951957823794E-5 0.0 0.001159580575188108 0.0 30-31 0.0 6.0237951957823794E-5 0.0 0.002921540669954454 0.0 32-33 0.0 6.0237951957823794E-5 0.0 0.008418253786105875 0.0 34-35 0.0 6.0237951957823794E-5 0.0 0.016670853204327735 0.0 36-37 0.0 6.0237951957823794E-5 0.0 0.02900457386769216 0.0 38-39 3.0118975978911897E-5 7.529743994727975E-5 0.0 0.0491240498216053 0.0 40-41 3.0118975978911897E-5 9.03569279367357E-5 0.0 0.07320417111674538 0.0 42-43 4.517846396836784E-5 9.03569279367357E-5 0.0 0.09866976530691537 0.0 44-45 6.0237951957823794E-5 9.03569279367357E-5 0.0 0.12961701312524734 0.0 46-47 6.0237951957823794E-5 9.03569279367357E-5 0.0 0.16821448084222296 0.0 48-49 6.0237951957823794E-5 9.03569279367357E-5 0.0 0.20861908711793326 0.0 50-51 6.0237951957823794E-5 9.03569279367357E-5 0.0 0.25245725665523955 0.0 52-53 6.0237951957823794E-5 1.2047590391564759E-4 0.0 0.29676227032021896 0.0 54-55 6.0237951957823794E-5 1.2047590391564759E-4 0.0 0.34951565674728313 0.0 56-57 6.0237951957823794E-5 1.2047590391564759E-4 0.0 0.4124944355191879 0.0 58-59 6.0237951957823794E-5 1.2047590391564759E-4 0.0 0.48191867515057984 0.0 60-61 6.0237951957823794E-5 1.2047590391564759E-4 0.0 0.5584811120889739 0.0 62-63 6.0237951957823794E-5 1.2047590391564759E-4 0.0 0.638416874337006 0.0 64-65 6.0237951957823794E-5 1.2047590391564759E-4 0.0 0.7276443406745325 0.0 66-67 6.0237951957823794E-5 1.505948798945595E-4 0.0 0.8339643258800915 0.0 68-69 6.0237951957823794E-5 1.505948798945595E-4 0.0 0.9557504052508218 0.0 70-71 6.0237951957823794E-5 1.505948798945595E-4 0.0 1.0945838250256161 0.0 72-73 6.0237951957823794E-5 1.6565436788401546E-4 0.0 1.2356611085108395 0.0 74-75 6.0237951957823794E-5 1.807138558734714E-4 0.0 1.3819489748404146 0.0 76-77 6.0237951957823794E-5 1.9577334386292733E-4 0.0 1.5455401928698747 0.0 78-79 6.0237951957823794E-5 2.1083283185238327E-4 0.0 1.7365246195521549 0.0 80-81 6.0237951957823794E-5 2.258923198418392E-4 0.0 1.9481254852920005 0.0 82-83 6.0237951957823794E-5 2.4095180783129518E-4 0.0 2.1734606040782296 0.0 84-85 6.0237951957823794E-5 2.4095180783129518E-4 0.0 2.408072367465964 0.0 86-87 6.0237951957823794E-5 2.4095180783129518E-4 0.0 2.6648215781981985 0.0 88 6.0237951957823794E-5 2.4095180783129518E-4 0.0 2.875398398754761 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 215 1.5594336E-5 18.579515 1 GTATAGG 1080 0.0 18.031162 1 GTATCAA 13280 0.0 16.619083 1 CATGGGG 5795 0.0 16.386667 4 GTACATG 13970 0.0 16.048439 1 ACATGGG 13530 0.0 15.947329 3 TACATGG 13800 0.0 15.689564 2 GTATATA 2410 0.0 15.539144 1 CAACGCA 14585 0.0 14.987846 5 TATCAAC 14670 0.0 14.963142 2 ATCAACG 14665 0.0 14.873356 3 TCAACGC 14730 0.0 14.840309 4 AACGCAG 14820 0.0 14.795769 6 CTGGTCG 385 9.3279596E-8 14.632682 9 GAGTACT 4200 0.0 14.587173 12-13 CCTATAC 1160 0.0 14.18349 3 TATACCG 200 0.005656492 14.103081 5 AGTACTT 5135 0.0 13.896746 12-13 ACGCAGA 15865 0.0 13.671777 7 CGCAGAG 15990 0.0 13.506175 8 >>END_MODULE