FastQCFastQC Report
Wed 25 May 2016
SRR1295145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3322492
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG850.00986914926.2560044
GTACATG77500.020.269511
TACATGG78100.019.4789262
GTATATA20000.017.5569731
ACATGGG84300.017.074053
GTATAGG9050.016.3368151
GTATCAA50250.016.3049831
CATGGGG41300.015.6709564
ATCAACG47900.015.257393
TCAACGC47750.015.189994
CAACGCA46650.015.1889325
GTCTAGA9850.015.0099681
CCGTGCG2000.003755258415.0002779
GTATAGA12800.014.9195771
GTCCTAC6757.8216544E-1114.602291
AACGCAG48250.014.59557156
CTACGCT2707.954208E-414.4651144
GTGTTAG15250.014.1384881
TATACTG16000.014.0340525
TATCAAC55100.013.9726092
Produced by FastQC (version 0.11.5)