FastQCFastQC Report
Wed 25 May 2016
SRR1295145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3322492
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70200.025.8580441
CAACGCA76500.023.3025
TCAACGC77000.023.0896034
ATCAACG77450.022.9540673
AACGCAG79000.022.5309526
TATCAAC82100.021.9710542
ACGCAGA89050.019.8260567
CGCAGAG89000.019.6789198
CCGTGCG1708.698334E-519.3341469
GTACATG98800.018.1218261
TAGTACG2654.4482658E-717.7488234
GTATTAG14550.017.3843671
TACATGG102250.017.302052
GTATAGG8700.017.1022681
GAGTACT34900.016.75019312-13
GCAGAGT105950.016.5304159
TATACTG18050.016.4164335
CATGGGG48900.016.3514214
TACCGTT2304.7188245E-516.3321957
ACATGGG102000.016.2304943
Produced by FastQC (version 0.11.5)