FastQCFastQC Report
Wed 25 May 2016
SRR1295144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259135
Sequences flagged as poor quality0
Sequence length100
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41450.026.1396351
ATCAACG43650.024.2126793
TCAACGC43500.024.169754
GTACATG70850.023.8079571
TATCAAC45600.023.2161792
CAACGCA43050.023.127135
TACATGG72450.022.5980222
AACGCAG44250.022.5509056
GGTATCA30100.021.6795751
ACATGGG75850.021.1588293
CGCAGAG46800.021.1245678
CATGGGT20150.020.2605574
ACGCAGA47400.020.1640017
GCAGAGT54650.017.909389
GTGTACG1750.00941261417.589091
TCTGTCG4001.9645086E-1017.5644868
GTCTTGC11950.017.5154951
GCGTTAA2150.0015178417.180041
GTATAAG9600.016.672991
CATGGGG29650.016.4096054
Produced by FastQC (version 0.11.5)