##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295144_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2259135 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.65291228722498 33.0 27.0 33.0 15.0 33.0 2 22.81259774205614 27.0 6.0 33.0 2.0 33.0 3 25.03965854187554 27.0 15.0 33.0 15.0 33.0 4 25.097928631976398 33.0 6.0 37.0 2.0 37.0 5 26.973067567896564 33.0 15.0 37.0 2.0 37.0 6 28.162499806341806 33.0 15.0 37.0 6.0 37.0 7 27.88881939326335 33.0 15.0 37.0 6.0 37.0 8 28.60092911667519 33.0 22.0 37.0 6.0 37.0 9 29.614432515099807 37.0 27.0 37.0 15.0 37.0 10-11 29.197551717803496 35.0 24.5 37.0 10.5 37.0 12-13 27.990861989212686 33.0 18.5 37.0 4.0 37.0 14-15 29.825293973135736 35.0 27.0 37.0 10.5 40.0 16-17 30.27154286928404 37.0 27.0 40.0 6.0 40.0 18-19 30.431667208909605 37.0 27.0 40.0 10.5 40.0 20-21 30.002568018290184 35.0 27.0 40.0 6.0 40.0 22-23 30.72443368811514 37.0 27.0 40.0 15.0 40.0 24-25 30.8818255659799 37.0 27.0 40.0 15.0 40.0 26-27 29.531323493283935 35.0 24.5 40.0 4.0 40.0 28-29 30.219667925998223 37.0 27.0 40.0 10.5 40.0 30-31 30.913315494647286 37.0 27.0 40.0 15.0 40.0 32-33 29.722510164288543 33.0 27.0 40.0 6.0 40.0 34-35 29.3785046046385 33.0 24.5 37.0 6.0 40.0 36-37 29.663051787520445 33.0 27.0 37.0 6.0 40.0 38-39 29.789261376588826 33.0 24.5 37.0 6.0 40.0 40-41 28.898441881516597 33.0 22.0 37.0 6.0 40.0 42-43 29.510650315275537 33.0 22.0 37.0 6.0 40.0 44-45 29.430977121774482 33.0 22.0 37.0 6.0 40.0 46-47 29.10461415541789 33.0 22.0 37.0 6.0 40.0 48-49 29.026284839108776 33.0 22.0 37.0 10.5 40.0 50-51 28.997883039304867 33.0 22.0 37.0 6.0 40.0 52-53 29.159006655202102 33.0 22.0 37.0 6.0 40.0 54-55 29.149306924995628 33.0 22.0 37.0 6.0 40.0 56-57 29.151507988677082 33.0 22.0 37.0 6.0 40.0 58-59 29.054407992439586 33.0 22.0 37.0 10.5 40.0 60-61 29.00243898660328 33.0 22.0 37.0 10.5 40.0 62-63 28.966744129943542 33.0 22.0 37.0 10.5 40.0 64-65 28.534684956852956 33.0 22.0 37.0 6.0 40.0 66-67 28.720020494569823 33.0 22.0 37.0 10.5 40.0 68-69 28.304399914126424 33.0 22.0 37.0 10.5 40.0 70-71 28.452075683834742 33.0 22.0 37.0 10.5 37.0 72-73 28.48867354097918 33.0 22.0 37.0 10.5 37.0 74-75 28.583714341993726 33.0 22.0 37.0 15.0 37.0 76-77 28.365409548344832 33.0 24.5 37.0 15.0 37.0 78-79 27.933323595092812 33.0 22.0 37.0 15.0 37.0 80-81 27.721324976152378 33.0 22.0 37.0 15.0 37.0 82-83 27.793694931909776 33.0 22.0 35.0 15.0 37.0 84-85 27.746842043525508 33.0 22.0 33.0 15.0 37.0 86-87 27.510561121845306 27.0 22.0 33.0 15.0 37.0 88-89 27.45688239082658 27.0 22.0 33.0 15.0 37.0 90-91 27.589857622497107 27.0 22.0 33.0 15.0 37.0 92-93 27.507845480681766 27.0 22.0 33.0 15.0 37.0 94-95 27.377943770513937 27.0 22.0 33.0 15.0 37.0 96-97 27.462498035752624 27.0 22.0 33.0 15.0 37.0 98-99 27.510178231933903 27.0 22.0 33.0 15.0 37.0 100 27.187359321156105 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.249284133293198 1101 2 11.254372143625794 1101 3 8.322840598667092 1101 4 12.78991606886898 1101 5 10.713902093348562 1101 6 10.019518981301122 1101 7 9.447163995931803 1101 8 9.003279187807248 1101 9 7.426227763598934 1101 10-11 7.800749259327077 1101 12-13 9.211373448598174 1101 14-15 8.112015644601577 1101 16-17 8.23954494218209 1101 18-19 8.162645822226 1101 20-21 7.228685856661237 1101 22-23 7.355557709498466 1101 24-25 7.311229174819626 1101 26-27 8.524419798885287 1101 28-29 8.21038045998161 1101 30-31 5.460004040222113 1101 32-33 7.064168121906459 1101 34-35 8.774113988080401 1101 36-37 8.15840642664297 1101 38-39 8.172211587566121 1101 40-41 9.15629342234962 1101 42-43 8.627287474286593 1101 44-45 8.544611357066156 1101 46-47 7.403117660320685 1101 48-49 5.408557150675119 1101 50-51 6.784790316570465 1101 52-53 5.9433394580238215 1101 54-55 3.995122147796433 1101 56-57 6.1919224850930945 1101 58-59 6.272790772954071 1101 60-61 6.823779173657915 1101 62-63 6.2681926002322506 1101 64-65 6.850103957386779 1101 66-67 5.805581909290446 1101 68-69 6.8347069257043565 1101 70-71 6.295954592926666 1101 72-73 5.392803157303046 1101 74-75 4.773761031037889 1101 76-77 5.069761377834446 1101 78-79 5.07468779695872 1101 80-81 4.829327495802378 1101 82-83 4.318029530313776 1101 84-85 4.134809060313486 1101 86-87 4.015421691833307 1101 88-89 3.972005124883392 1101 90-91 3.7584594339509394 1101 92-93 3.6990211706306333 1101 94-95 3.6467353516309693 1101 96-97 3.705794240087105 1101 98-99 3.4328731254217573 1101 100 3.6182429311258666 1102 1 6.8090454080330645 1102 2 10.634725782979995 1102 3 8.190240645222659 1102 4 12.775180453852858 1102 5 10.552164710552919 1102 6 9.708692011778812 1102 7 6.916262197283746 1102 8 7.931787146104956 1102 9 6.593669808057495 1102 10-11 7.543193869477172 1102 12-13 9.236326032452155 1102 14-15 6.465669181320095 1102 16-17 7.655130200292259 1102 18-19 7.614904621745556 1102 20-21 7.256923769405574 1102 22-23 6.7638370857437415 1102 24-25 7.031139992264595 1102 26-27 8.607210853219609 1102 28-29 7.8275984206728815 1102 30-31 4.3430094892775095 1102 32-33 4.77882623257695 1102 34-35 5.323732275767963 1102 36-37 5.5625227426998975 1102 38-39 4.7976077050629975 1102 40-41 6.524366446383986 1102 42-43 7.170731031119008 1102 44-45 6.571761067554245 1102 46-47 5.8400088301452335 1102 48-49 3.9781823960206246 1102 50-51 5.137502912499045 1102 52-53 6.129259095349376 1102 54-55 5.628071889851157 1102 56-57 6.566388293672688 1102 58-59 6.263092057757714 1102 60-61 6.380176042447037 1102 62-63 4.542294473792889 1102 64-65 6.427035766641836 1102 66-67 4.02822836774947 1102 68-69 6.467624009855914 1102 70-71 5.5666217898763755 1102 72-73 5.226572885545163 1102 74-75 4.511686573746847 1102 76-77 3.629150216069256 1102 78-79 4.627694522641239 1102 80-81 4.73510447024745 1102 82-83 4.134749907328807 1102 84-85 3.2613600198420016 1102 86-87 3.4774297781548036 1102 88-89 3.6708661584442446 1102 90-91 3.493369737455197 1102 92-93 3.333417569531111 1102 94-95 3.20694300454117 1102 96-97 3.498733184171236 1102 98-99 3.2717449489291432 1102 100 3.2819819946400344 1103 1 6.710672489874842 1103 2 10.919633486553266 1103 3 7.9375852955606945 1103 4 11.978452491729502 1103 5 10.361434343775453 1103 6 9.249997591463941 1103 7 8.249964490075264 1103 8 8.213989287483866 1103 9 7.301408681025574 1103 10-11 7.450882676756983 1103 12-13 8.751048012472218 1103 14-15 8.642957131062865 1103 16-17 8.08334610292351 1103 18-19 7.328758797861912 1103 20-21 8.504690515019995 1103 22-23 6.97022478882101 1103 24-25 5.322062573921354 1103 26-27 6.272603327588779 1103 28-29 7.137691604690762 1103 30-31 6.2425146327376915 1103 32-33 8.200223203965837 1103 34-35 7.302539589029557 1103 36-37 4.3215071148955815 1103 38-39 6.5000051434162 1103 40-41 6.993786769414953 1103 42-43 7.671756594559913 1103 44-45 5.833213746708967 1103 46-47 6.485226369925311 1103 48-49 5.803777506307089 1103 50-51 6.602472349342037 1103 52-53 6.246463956607791 1103 54-55 5.156616266471744 1103 56-57 6.065675589795937 1103 58-59 5.646479998069818 1103 60-61 5.210729181419666 1103 62-63 2.8857265970544965 1103 64-65 5.293424133780864 1103 66-67 4.2452354707629105 1103 68-69 5.465503050435142 1103 70-71 3.9915503012620164 1103 72-73 4.623820157833979 1103 74-75 3.531591070407611 1103 76-77 3.748140497925135 1103 78-79 3.0856228245292634 1103 80-81 4.213698839134246 1103 82-83 3.4740498165465077 1103 84-85 3.396311421779167 1103 86-87 2.9134166310372223 1103 88-89 3.0973284363098728 1103 90-91 2.8329613769992115 1103 92-93 2.4316038936767903 1103 94-95 2.949545099549969 1103 96-97 3.0759271210426213 1103 98-99 2.7783101661572864 1103 100 2.986333092087751 1104 1 6.340671875666516 1104 2 9.724185816427685 1104 3 7.405899739175695 1104 4 12.236342622009165 1104 5 9.55290923345419 1104 6 8.457999156682742 1104 7 4.109632061630535 1104 8 6.70785032588741 1104 9 6.71308678929093 1104 10-11 7.264238434318834 1104 12-13 7.6763853377097035 1104 14-15 7.516335764510082 1104 16-17 7.439071941925338 1104 18-19 7.063811472975406 1104 20-21 7.5543973486613325 1104 22-23 6.551465820367017 1104 24-25 6.107270167898601 1104 26-27 8.434545891348332 1104 28-29 7.39634215525091 1104 30-31 6.489736943078935 1104 32-33 7.914889899768031 1104 34-35 8.207104650227656 1104 36-37 6.77887759793331 1104 38-39 7.43779659707857 1104 40-41 7.493205549475725 1104 42-43 7.522708187181852 1104 44-45 6.800669268914287 1104 46-47 6.817505227929789 1104 48-49 5.7483784463833025 1104 50-51 5.922535801786786 1104 52-53 6.1940222085303915 1104 54-55 4.897716657810193 1104 56-57 6.075821290472653 1104 58-59 3.9047319038030146 1104 60-61 4.412921162693738 1104 62-63 3.9521704810244707 1104 64-65 2.862149742623302 1104 66-67 3.3174539676128987 1104 68-69 4.2087834594843905 1104 70-71 2.728693689208143 1104 72-73 2.587333610008745 1104 74-75 3.257129759795035 1104 76-77 4.085497542624651 1104 78-79 4.49587880428604 1104 80-81 3.235573092466158 1104 82-83 3.2485265245518704 1104 84-85 3.120819624835093 1104 86-87 0.6816902567967134 1104 88-89 0.48026526498354727 1104 90-91 2.4151088720416745 1104 92-93 2.631937852580176 1104 94-95 2.591057390128551 1104 96-97 2.892235001740417 1104 98-99 2.558819910133657 1104 100 0.8819008871878538 1105 1 5.916995647195893 1105 2 -2.9095095851019686 1105 3 0.3730483392521293 1105 4 8.33319237069535 1105 5 8.645796979950553 1105 6 8.305924064082827 1105 7 5.331857329755042 1105 8 6.225228631788809 1105 9 5.211814497989437 1105 10-11 5.164156759344916 1105 12-13 5.381231896798681 1105 14-15 6.135287744785536 1105 16-17 6.376731683822744 1105 18-19 6.48709303313672 1105 20-21 7.308679842481283 1105 22-23 2.4179635200836955 1105 24-25 4.429654512749206 1105 26-27 7.267666907375332 1105 28-29 6.295530807509021 1105 30-31 5.5405830409824794 1105 32-33 7.474729113753238 1105 34-35 6.586598184762543 1105 36-37 6.511969346422767 1105 38-39 4.423803811566888 1105 40-41 5.897936086563259 1105 42-43 6.058393620010843 1105 44-45 6.547407913835361 1105 46-47 5.84319172503287 1105 48-49 5.446031076994732 1105 50-51 6.277187808346589 1105 52-53 5.892186819481772 1105 54-55 5.595148774054721 1105 56-57 5.3670151479986 1105 58-59 2.0989155652606506 1105 60-61 4.850328542628301 1105 62-63 -0.7179694548933284 1105 64-65 -0.23052958352994324 1105 66-67 1.166928119459012 1105 68-69 3.2344405277962736 1105 70-71 3.4920019529739648 1105 72-73 3.0280180672531465 1105 74-75 3.020125191479117 1105 76-77 2.109933114534911 1105 78-79 3.512450035667289 1105 80-81 1.8807541883782157 1105 82-83 1.270886761981746 1105 84-85 1.6962028844486312 1105 86-87 -0.06922969527870038 1105 88-89 1.9060764917851927 1105 90-91 1.527388579398547 1105 92-93 1.9761212721573393 1105 94-95 1.1274575797628472 1105 96-97 2.145412504921147 1105 98-99 2.1963265416900306 1105 100 2.2950198391681305 1106 1 6.042159513117774 1106 2 10.292740044250039 1106 3 6.812318534398209 1106 4 11.696110138651438 1106 5 9.842344310065041 1106 6 9.419518566758615 1106 7 6.741868768104528 1106 8 7.661507706234762 1106 9 6.927419654594733 1106 10-11 5.706726547762258 1106 12-13 7.959331582893526 1106 14-15 7.911500234146381 1106 16-17 7.852965113771798 1106 18-19 7.247652640637444 1106 20-21 8.324902168681362 1106 22-23 6.3162098019342885 1106 24-25 5.1253836718864925 1106 26-27 7.817808622120207 1106 28-29 6.358904538276821 1106 30-31 5.407191182348992 1106 32-33 7.518097371204927 1106 34-35 6.941437345624372 1106 36-37 6.099329486008955 1106 38-39 7.202425071268383 1106 40-41 6.8345976655010645 1106 42-43 6.40561066446919 1106 44-45 4.556909695344057 1106 46-47 6.164879145728914 1106 48-49 5.950940830250342 1106 50-51 6.664942805625934 1106 52-53 5.711519483918906 1106 54-55 5.344726023980229 1106 56-57 5.041746109448006 1106 58-59 5.11054050128649 1106 60-61 4.96672915894165 1106 62-63 4.94053169860555 1106 64-65 5.203920764061792 1106 66-67 3.0958063710537367 1106 68-69 3.5946064040229295 1106 70-71 3.1045127864447046 1106 72-73 2.8014761709677494 1106 74-75 2.8871219474336876 1106 76-77 3.6316955730314575 1106 78-79 4.164789281841859 1106 80-81 3.8170213543713665 1106 82-83 2.188968161847182 1106 84-85 2.892353286919004 1106 86-87 3.518372645195729 1106 88-89 3.4065517076347334 1106 90-91 3.1950875890766497 1106 92-93 2.889417801651792 1106 94-95 1.4535994684168578 1106 96-97 1.5800181819653183 1106 98-99 1.9522034254960765 1106 100 2.9406400370014047 1107 1 6.137513652507106 1107 2 10.416532504032137 1107 3 6.064669028076501 1107 4 11.359397649379968 1107 5 9.411296891943476 1107 6 8.203065707961354 1107 7 3.7022275885828577 1107 8 6.369901978293086 1107 9 5.873389930094152 1107 10-11 4.552282676224742 1107 12-13 6.233926410845683 1107 14-15 6.326497573569938 1107 16-17 7.08491056557067 1107 18-19 6.397015341793274 1107 20-21 5.719778681122481 1107 22-23 6.0348055274682615 1107 24-25 4.781772421486874 1107 26-27 7.428021032129887 1107 28-29 6.615024693192179 1107 30-31 5.1954247395057 1107 32-33 5.060841990025324 1107 34-35 6.84183577710305 1107 36-37 6.709064397580647 1107 38-39 6.5048299289221525 1107 40-41 7.850407077690271 1107 42-43 6.390497280302181 1107 44-45 4.341003792694789 1107 46-47 3.6615146648306727 1107 48-49 4.311672670344475 1107 50-51 5.147404661300477 1107 52-53 4.136568023478834 1107 54-55 5.689846544196293 1107 56-57 5.6107655788317246 1107 58-59 3.2253155428740605 1107 60-61 3.187730948467422 1107 62-63 0.8104148355420797 1107 64-65 2.4378104306448876 1107 66-67 4.390780062106508 1107 68-69 5.257782794851909 1107 70-71 4.544163881982033 1107 72-73 2.682208245696703 1107 74-75 2.8063839612338626 1107 76-77 3.4142024031432463 1107 78-79 4.09106467961006 1107 80-81 2.346370128333966 1107 82-83 1.7260590350004499 1107 84-85 2.5639248162140333 1107 86-87 2.6030422777128344 1107 88-89 2.8234832712080014 1107 90-91 1.6478152229892906 1107 92-93 2.3980406312382634 1107 94-95 2.4224465972826117 1107 96-97 2.546460801398588 1107 98-99 1.9854200052069153 1107 100 0.24798988564122126 1108 1 4.495362764346044 1108 2 -3.196588415945026 1108 3 0.10208640575987005 1108 4 6.704838951110801 1108 5 7.101262911805794 1108 6 6.967304336638566 1108 7 4.300768834144357 1108 8 5.3119860087985735 1108 9 5.049052054099157 1108 10-11 3.400878290500984 1108 12-13 2.662716250196123 1108 14-15 4.157549748911865 1108 16-17 4.869050883909697 1108 18-19 4.0813651908546795 1108 20-21 4.958704727803678 1108 22-23 4.290379010896331 1108 24-25 3.1603496727760607 1108 26-27 4.905381108195815 1108 28-29 6.259993071573053 1108 30-31 4.555119853598011 1108 32-33 4.523805625987411 1108 34-35 3.0931483726139817 1108 36-37 1.3993752745539894 1108 38-39 2.596226437259869 1108 40-41 5.600005420826868 1108 42-43 3.2222098944043758 1108 44-45 3.867599426913781 1108 46-47 4.8284677818988975 1108 48-49 4.930309903161078 1108 50-51 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26-27 -17.817856687194638 2212 28-29 -18.718801904495486 2212 30-31 -6.361126510582707 2212 32-33 -17.842453624316317 2212 34-35 -14.281073114864519 2212 36-37 -18.306448662353706 2212 38-39 -24.445129221404645 2212 40-41 -10.723808275754312 2212 42-43 -14.470822109537353 2212 44-45 -17.807204074156598 2212 46-47 -17.8109571798376 2212 48-49 -18.42146000294551 2212 50-51 -9.54311921560653 2212 52-53 -2.3021588318399964 2212 54-55 -24.355598717241612 2212 56-57 -24.2943610551552 2212 58-59 -13.417899290003714 2212 60-61 -6.84617818064147 2212 62-63 -3.5793971363504333 2212 64-65 -18.192570103172713 2212 66-67 -18.48583182283419 2212 68-69 -15.27920806636633 2212 70-71 -10.323156067427815 2212 72-73 -18.405848477363836 2212 74-75 -6.900036705112452 2212 76-77 -12.085898615940389 2212 78-79 -5.270923945407102 2212 80-81 -2.899370493662765 2212 82-83 -3.4019899991687055 2212 84-85 -5.555087625384715 2212 86-87 -5.281031888236171 2212 88-89 -3.6485597949045037 2212 90-91 -4.458289397938223 2212 92-93 -5.515276024195391 2212 94-95 -4.023928260744622 2212 96-97 -5.909109653496721 2212 98-99 -4.040788011417941 2212 100 -4.044760948513787 2213 1 -21.34022327559747 2213 2 -17.193965861980935 2213 3 -20.07503685427644 2213 4 -19.02826574931284 2213 5 -21.209005395638226 2213 6 -22.20134605674453 2213 7 -10.89731060239975 2213 8 -10.712455117087437 2213 9 -4.58555268650051 2213 10-11 -18.025875211224022 2213 12-13 -16.672612652519778 2213 14-15 -24.082918276103264 2213 16-17 -18.371015060329402 2213 18-19 -19.02119703496534 2213 20-21 -18.187553142850046 2213 22-23 -20.350008779610505 2213 24-25 -7.78214866248344 2213 26-27 -23.878964261987765 2213 28-29 -24.801233476742464 2213 30-31 -20.067787360305978 2213 32-33 -24.055375330236114 2213 34-35 -18.63787785976413 2213 36-37 -24.489771386733082 2213 38-39 -12.063773289201256 2213 40-41 -17.737959766222435 2213 42-43 -23.981643242573636 2213 44-45 -17.842383545291252 2213 46-47 -17.699987633828904 2213 48-49 -6.553350316251294 2213 50-51 -24.17806510994135 2213 52-53 -18.560232200848183 2213 54-55 -18.43425337825856 2213 56-57 -18.368248767019608 2213 58-59 -18.872853491688918 2213 60-61 -20.240688653836777 2213 62-63 -18.35886378692386 2213 64-65 -18.522522113389677 2213 66-67 -9.435477276987324 2213 68-69 -23.628348741095802 2213 70-71 -18.308946924487447 2213 72-73 -10.094099982396795 2213 74-75 -4.791262335985589 2213 76-77 -3.7566859733938074 2213 78-79 -4.957497965787081 2213 80-81 -3.771427520386542 2213 82-83 -3.7692138269691995 2213 84-85 -3.514149987153644 2213 86-87 -3.585548200851477 2213 88-89 -3.275807398449693 2213 90-91 -3.167506173931063 2213 92-93 -4.22547424081225 2213 94-95 -4.19713735918133 2213 96-97 -3.8035655085604247 2213 98-99 -4.1709234643843125 2213 100 -3.8179338708670336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 565.0 3 543.0 4 2634.0 5 10425.0 6 15996.0 7 19219.0 8 17206.0 9 16992.0 10 19466.0 11 22876.0 12 29063.0 13 39677.0 14 28523.0 15 31058.0 16 36988.0 17 40707.0 18 41493.0 19 42002.0 20 41710.0 21 40774.0 22 42710.0 23 46752.0 24 52904.0 25 58814.0 26 64943.0 27 70361.0 28 77117.0 29 81102.0 30 85663.0 31 93731.0 32 107572.0 33 132409.0 34 164128.0 35 197245.0 36 222593.0 37 198902.0 38 64098.0 39 174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.8601260243185 21.92484294819947 14.288291875614402 22.926739151867633 2 14.954835307900344 21.526340202075698 41.4936095496218 22.02521494040216 3 17.933707026855313 27.6762581756259 29.77405414424168 24.61598065327711 4 11.708042961907566 18.312574062494374 40.22044133553728 29.758941640060776 5 13.09700738044687 38.135325042968354 35.642149428773635 13.12551814781114 6 29.311091261619772 39.02542472265005 19.269566950987826 12.393917064742352 7 25.330656890438124 32.37723420878659 24.581188773363785 17.7109201274115 8 23.012796673777547 36.765409849914114 22.39714062941497 17.824652846893365 9 23.910007271222206 17.630302596149388 23.042844962840917 35.416845169787486 10-11 22.47465795776429 28.3057122749872 29.446501609429077 19.773128157819432 12-13 23.214365746160563 26.114917892221445 29.842482495182082 20.828233866435912 14-15 20.85317451777373 27.481050742259967 27.23544808436622 24.43032665560008 16-17 20.087081307093396 31.339308390637775 27.831378748953973 20.742231553314856 18-19 19.8292582300331 30.09583922152565 29.903852537534263 20.17105001090699 20-21 22.097812097812096 29.19377533183503 28.550034576153976 20.15837799419889 22-23 20.804784948812546 29.296044516220554 29.43398335808858 20.46518717687832 24-25 20.853757066605866 29.07995860883596 28.960275005700055 21.106009318858117 26-27 20.727474737949667 29.128891362251313 29.116261253195084 21.02737264660393 28-29 20.45904835079158 29.040107878038878 29.531306188938157 20.969537582231386 30-31 21.243787307952942 29.508951595930437 28.679058515522186 20.56820258059443 32-33 20.17413757347996 29.291152169657547 29.614386470190347 20.920323786672146 34-35 20.470805622426845 28.60046528129993 29.32160367272199 21.607125423551235 36-37 20.979971580057875 29.5480772274207 28.715398149300604 20.756553043220823 38-39 20.609969835234057 29.11261175284426 29.15365673357736 21.123761678344323 40-41 20.98065034847422 29.061975397476335 28.741648888847067 21.21572536520238 42-43 20.413715406311844 29.222417948579753 29.429868976805007 20.93399766830339 44-45 20.973102025004934 28.696093176586647 29.147673968712123 21.1831308296963 46-47 20.847882702468926 29.357289257441565 29.070203644158628 20.724624395930878 48-49 20.545480543205635 28.95702151008293 29.298234666879114 21.19926327983232 50-51 21.122994020635026 28.946803830233858 28.896641055218748 21.033561093912372 52-53 20.44831661610464 29.074276796134196 29.28011825161574 21.197288336145427 54-55 20.9802045995072 28.971199874075417 28.89435979876283 21.15423572765456 56-57 21.022849336114675 29.102585325795317 28.827210323819052 21.047355014270956 58-59 20.49959235645451 28.55252189366458 29.419657579421788 21.528228170459126 60-61 21.167164876516914 29.08958943987313 28.66434478812378 21.078900895486175 62-63 20.548367860967804 29.217592445735903 29.280509654493926 20.953530038802366 64-65 20.64935542792241 28.452117552396434 29.25107822085286 21.647448798828297 66-67 20.740491780622026 28.890176891879836 28.880350447761327 21.488980879736804 68-69 21.139009753994728 28.816961230919304 28.947847590830683 21.09618142425529 70-71 21.39192484767878 28.806680004584674 28.601663419190032 21.19973172854651 72-73 20.71135160764078 29.099995119194006 29.28164207407714 20.90701119908808 74-75 21.026040883769852 28.500396115543786 28.84402383706529 21.629539163621068 76-77 21.113540342648456 29.108201006104657 28.69797167869099 21.0802869725559 78-79 21.01722423204495 28.780005957791545 29.001205293202826 21.20156451696068 80-81 21.326079412471323 28.65077795880657 28.700660164409943 21.322482464312166 82-83 20.62661815909294 29.006673511293634 29.404517453798768 20.96219087581466 84-85 20.88751160042642 28.37870961584048 28.910211915312743 21.823566868420357 86-87 21.11460708950011 28.980736427452168 28.79712341090431 21.107533072143408 88-89 20.78693968692203 28.468265051516788 29.145108446541855 21.599686815019325 90-91 21.205550939367708 28.47015292321606 28.769130941405592 21.555165196010638 92-93 20.730840920997608 28.8814125747616 29.136275479350353 21.251471024890442 94-95 20.7845208031316 28.21568311421865 28.985493373131348 22.0143027095184 96-97 20.854838106666048 28.671507948235437 28.984799633966357 21.488854311132158 98-99 20.77138788788143 28.430151427060252 29.070272280453896 21.72818840460442 100 21.096609273419546 28.24102584852647 28.631201514281763 22.031163363772222 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 279.5 2 261.5 3 223.5 4 287.5 5 413.0 6 627.0 7 920.5 8 1392.5 9 2043.5 10 2728.5 11 3505.5 12 4405.5 13 5175.0 14 5848.5 15 6571.0 16 7286.0 17 8001.5 18 8622.5 19 9281.5 20 10110.5 21 11186.0 22 12571.5 23 14254.0 24 16234.5 25 19100.5 26 23174.5 27 28238.0 28 34411.0 29 41425.5 30 48950.0 31 56528.0 32 64035.5 33 70929.0 34 77281.5 35 83029.0 36 87345.0 37 90698.0 38 92662.0 39 93709.5 40 93093.0 41 90899.0 42 87984.0 43 84073.5 44 78977.0 45 73635.0 46 68944.5 47 64374.5 48 60451.5 49 57302.5 50 54590.5 51 50809.5 52 45985.5 53 41743.0 54 38052.0 55 34908.5 56 32139.0 57 28808.5 58 25291.0 59 21708.0 60 18087.5 61 14753.5 62 11853.5 63 9231.5 64 7058.0 65 5428.0 66 4015.5 67 3017.5 68 2220.0 69 1566.5 70 1151.0 71 826.5 72 614.0 73 466.0 74 348.5 75 255.0 76 181.0 77 128.5 78 81.0 79 51.5 80 41.0 81 31.5 82 23.5 83 16.5 84 14.5 85 9.0 86 5.5 87 3.5 88 2.0 89 3.0 90 2.5 91 2.0 92 1.5 93 0.5 94 1.0 95 0.5 96 1.0 97 1.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.5115918260750245 2 23.941375792062004 3 6.083390324172747 4 23.760067459447974 5 12.435511822002669 6 9.411699610691702 7 7.934895435642403 8 8.825546060771048 9 5.4586379300041825 10-11 6.722307431826784 12-13 10.123078080769853 14-15 7.443379877696551 16-17 7.801835658338257 18-19 6.657194014523257 20-21 7.824897582481791 22-23 5.767384419257813 24-25 5.355921624869696 26-27 9.403532768072735 28-29 6.266048731040863 30-31 4.303859663101143 32-33 8.645211552209142 34-35 7.0526993738754 36-37 7.000267801614335 38-39 5.959648272458264 40-41 8.278854517326321 42-43 6.371332390494593 44-45 6.129492040094992 46-47 5.838296516144453 48-49 3.8784092141461226 50-51 6.330918692331357 52-53 4.531734491298661 54-55 4.949593539120061 56-57 4.807725080617139 58-59 3.3577010670013077 60-61 4.037231064101968 62-63 3.0171061047701886 64-65 4.360761973056059 66-67 2.4742434604395047 68-69 5.069484559355683 70-71 2.4851325839314606 72-73 1.5997937263598678 74-75 0.9083343846206623 76-77 1.0966586768829663 78-79 1.7047896650709233 80-81 2.1657846919285477 82-83 0.8381526557731167 84-85 1.2188735954247976 86-87 0.507849243183785 88-89 0.7246578889707787 90-91 0.3008009702828737 92-93 0.34209996303895074 94-95 0.5810631060118142 96-97 0.5465366168909782 98-99 0.49231232307940864 100 0.4491984764080057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2259135.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.56478519513 #Duplication Level Percentage of deduplicated Percentage of total 1 98.21869884754135 95.82686255205533 2 1.5448857840225425 3.0145289933833874 3 0.13783414042047112 0.40343274908035953 4 0.04396936396525043 0.17159446201744588 5 0.01987681175975481 0.09696384348522559 6 0.009320501106726683 0.05456116130332561 7 0.008163672127289792 0.05575408422517491 8 0.003872482176399651 0.030225431336992115 9 0.003245778936522831 0.028500635225945477 >10 0.008868187199942588 0.1358318897920616 >50 6.269646627690817E-4 0.039999366770205325 >100 5.465854688020845E-4 0.09129303854183718 >500 9.088061225962666E-5 0.05045179278274658 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.426472964209753E-5 0.0 3 0.0 0.0 0.0 4.426472964209753E-5 0.0 4 0.0 0.0 0.0 4.426472964209753E-5 0.0 5 0.0 0.0 0.0 4.426472964209753E-5 0.0 6 0.0 0.0 0.0 4.426472964209753E-5 0.0 7 0.0 0.0 0.0 4.426472964209753E-5 0.0 8 0.0 0.0 0.0 1.327941889262926E-4 0.0 9 0.0 0.0 0.0 1.7705891856839012E-4 0.0 10-11 0.0 0.0 0.0 1.7705891856839012E-4 0.0 12-13 0.0 0.0 0.0 1.7705891856839012E-4 0.0 14-15 0.0 0.0 0.0 1.7705891856839012E-4 0.0 16-17 0.0 0.0 0.0 1.7705891856839012E-4 0.0 18-19 0.0 0.0 0.0 1.9919128338943888E-4 0.0 20-21 0.0 0.0 0.0 2.4345601303153643E-4 0.0 22-23 0.0 0.0 0.0 2.655883778525852E-4 0.0 24-25 0.0 0.0 0.0 2.8772074267363395E-4 4.426472964209753E-5 26-27 0.0 0.0 0.0 3.9838256677887776E-4 4.426472964209753E-5 28-29 0.0 0.0 0.0 6.63970944631463E-4 4.426472964209753E-5 30-31 0.0 4.426472964209753E-5 0.0 0.0019919128338943886 4.426472964209753E-5 32-33 0.0 4.426472964209753E-5 0.0 0.006020003231325264 4.426472964209753E-5 34-35 0.0 4.426472964209753E-5 0.0 0.01179655044961899 4.426472964209753E-5 36-37 0.0 4.426472964209753E-5 0.0 0.02133559968749101 4.426472964209753E-5 38-39 0.0 4.426472964209753E-5 0.0 0.036252813576877874 4.426472964209753E-5 40-41 0.0 4.426472964209753E-5 0.0 0.05243157226106452 4.426472964209753E-5 42-43 0.0 4.426472964209753E-5 0.0 0.07002680229379829 4.426472964209753E-5 44-45 0.0 6.63970944631463E-5 0.0 0.09304446170768901 4.426472964209753E-5 46-47 0.0 8.852945928419506E-5 0.0 0.11953690239848438 4.426472964209753E-5 48-49 0.0 8.852945928419506E-5 0.0 0.1495926538254686 4.426472964209753E-5 50-51 0.0 1.1066182410524383E-4 0.0 0.1801353172785159 4.426472964209753E-5 52-53 0.0 1.327941889262926E-4 0.0 0.21207231971528928 4.426472964209753E-5 54-55 0.0 1.327941889262926E-4 0.0 0.2502506490315984 4.426472964209753E-5 56-57 0.0 1.327941889262926E-4 0.0 0.295489202725822 4.426472964209753E-5 58-59 0.0 1.327941889262926E-4 0.0 0.3482527604592023 4.426472964209753E-5 60-61 0.0 1.327941889262926E-4 0.0 0.40579690899392906 4.426472964209753E-5 62-63 0.0 1.327941889262926E-4 0.0 0.4671256919130552 4.426472964209753E-5 64-65 0.0 1.327941889262926E-4 0.0 0.5314202117182019 4.426472964209753E-5 66-67 0.0 1.327941889262926E-4 0.0 0.6120041520316404 4.426472964209753E-5 68-69 0.0 1.327941889262926E-4 0.0 0.7017508913809931 4.426472964209753E-5 70-71 0.0 1.327941889262926E-4 0.0 0.8090264636686165 4.426472964209753E-5 72-73 0.0 1.327941889262926E-4 0.0 0.9229417454025546 4.426472964209753E-5 74-75 0.0 1.327941889262926E-4 0.0 1.0425893096251442 4.426472964209753E-5 76-77 0.0 1.327941889262926E-4 0.0 1.1760474694960683 4.426472964209753E-5 78-79 0.0 1.327941889262926E-4 0.0 1.3284066689241678 4.426472964209753E-5 80-81 0.0 1.327941889262926E-4 0.0 1.5028097037140322 4.426472964209753E-5 82-83 0.0 1.327941889262926E-4 0.0 1.6973089257614087 4.426472964209753E-5 84-85 0.0 1.327941889262926E-4 0.0 1.8932024867925112 4.426472964209753E-5 86-87 0.0 1.327941889262926E-4 0.0 2.1071339251527688 4.426472964209753E-5 88 0.0 1.327941889262926E-4 0.0 2.2861847565550533 4.426472964209753E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4145 0.0 26.139635 1 ATCAACG 4365 0.0 24.212679 3 TCAACGC 4350 0.0 24.16975 4 GTACATG 7085 0.0 23.807957 1 TATCAAC 4560 0.0 23.216179 2 CAACGCA 4305 0.0 23.12713 5 TACATGG 7245 0.0 22.598022 2 AACGCAG 4425 0.0 22.550905 6 GGTATCA 3010 0.0 21.679575 1 ACATGGG 7585 0.0 21.158829 3 CGCAGAG 4680 0.0 21.124567 8 CATGGGT 2015 0.0 20.260557 4 ACGCAGA 4740 0.0 20.164001 7 GCAGAGT 5465 0.0 17.90938 9 GTGTACG 175 0.009412614 17.58909 1 TCTGTCG 400 1.9645086E-10 17.564486 8 GTCTTGC 1195 0.0 17.515495 1 GCGTTAA 215 0.00151784 17.18004 1 GTATAAG 960 0.0 16.67299 1 CATGGGG 2965 0.0 16.409605 4 >>END_MODULE