FastQCFastQC Report
Wed 25 May 2016
SRR1295144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259135
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68400.036.6304051
TATCAAC81900.030.502412
CAACGCA82850.029.7919275
GGTATCA42850.029.6999991
TCAACGC83300.029.6874284
ATCAACG83600.029.6358183
AACGCAG85300.028.6801646
ACGCAGA96950.025.085187
CGCAGAG97350.024.9821058
GCAGAGT108300.022.1961049
CTGTCGC3301.8189894E-1221.340899
GTATAGG5300.020.6354581
GAGTACT43500.020.23615612-13
GTACATG98800.020.076281
CCGTACT1200.00505658819.5893754
AGTACCG1708.611674E-519.3589125
TACATGG101100.019.3550872
ACATGGG100850.018.6930873
CATGGGT25000.018.6177434
TACCGCA1550.001041163118.174187
Produced by FastQC (version 0.11.5)