##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295144_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2259135 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47139458243974 33.0 33.0 33.0 27.0 33.0 2 30.146726512581143 33.0 33.0 33.0 27.0 33.0 3 30.936689485134796 33.0 33.0 33.0 27.0 33.0 4 35.20224554973474 37.0 37.0 37.0 33.0 37.0 5 35.53393489100917 37.0 37.0 37.0 33.0 37.0 6 35.346878783251114 37.0 37.0 37.0 33.0 37.0 7 35.25153875266418 37.0 37.0 37.0 33.0 37.0 8 35.25504451925184 37.0 37.0 37.0 33.0 37.0 9 35.19541860048204 37.0 37.0 37.0 33.0 37.0 10-11 35.17940295732659 37.0 37.0 37.0 33.0 37.0 12-13 35.10271763307638 37.0 37.0 37.0 33.0 37.0 14-15 36.58686621206789 40.0 37.0 40.0 33.0 40.0 16-17 36.599179995883375 40.0 37.0 40.0 33.0 40.0 18-19 36.645273080183344 40.0 37.0 40.0 33.0 40.0 20-21 36.64598839821436 40.0 37.0 40.0 33.0 40.0 22-23 36.593843440077734 40.0 37.0 40.0 33.0 40.0 24-25 36.57147824277877 40.0 37.0 40.0 33.0 40.0 26-27 36.55047219400346 40.0 37.0 40.0 33.0 40.0 28-29 36.501055492478315 40.0 37.0 40.0 33.0 40.0 30-31 36.40479254227835 37.0 37.0 40.0 33.0 40.0 32-33 36.36452757360671 37.0 37.0 40.0 33.0 40.0 34-35 36.272813267024766 37.0 37.0 40.0 33.0 40.0 36-37 36.26335234503472 37.0 37.0 40.0 33.0 40.0 38-39 36.19877696551999 37.0 37.0 40.0 27.0 40.0 40-41 36.12592540950408 37.0 37.0 40.0 27.0 40.0 42-43 36.028562923419805 37.0 37.0 40.0 27.0 40.0 44-45 35.9057077155637 37.0 37.0 40.0 27.0 40.0 46-47 35.77800264260436 37.0 37.0 40.0 27.0 40.0 48-49 35.65117401129193 37.0 37.0 40.0 27.0 40.0 50-51 35.540277584119586 37.0 37.0 40.0 27.0 40.0 52-53 35.44839153038663 37.0 37.0 40.0 27.0 40.0 54-55 35.34178723272403 37.0 35.0 40.0 27.0 40.0 56-57 35.2288911463901 37.0 33.0 40.0 27.0 40.0 58-59 35.11697021205019 37.0 33.0 40.0 27.0 40.0 60-61 34.9677268069416 37.0 33.0 40.0 27.0 40.0 62-63 34.80809026463669 37.0 33.0 40.0 27.0 40.0 64-65 34.63094038204888 37.0 33.0 40.0 27.0 40.0 66-67 34.41578413861943 37.0 33.0 40.0 27.0 40.0 68-69 34.20353896513488 37.0 33.0 38.5 27.0 40.0 70-71 33.97605167464539 37.0 33.0 37.0 27.0 40.0 72-73 33.76341475830351 37.0 33.0 37.0 24.5 40.0 74-75 33.52056008162416 37.0 33.0 37.0 22.0 40.0 76-77 33.28315306522187 37.0 33.0 37.0 22.0 38.5 78-79 33.045903409933445 37.0 33.0 37.0 22.0 37.0 80-81 32.82312035358666 37.0 33.0 37.0 22.0 37.0 82-83 32.60403893525619 37.0 33.0 37.0 22.0 37.0 84-85 32.429715798303334 37.0 33.0 37.0 22.0 37.0 86-87 32.440579469575745 37.0 33.0 37.0 22.0 37.0 88-89 32.44572126057097 37.0 33.0 37.0 22.0 37.0 90-91 32.44243084189303 37.0 33.0 37.0 22.0 37.0 92-93 32.4078474725946 35.0 33.0 37.0 22.0 37.0 94-95 32.42315465875213 37.0 33.0 37.0 22.0 37.0 96-97 32.37400907869605 33.0 33.0 37.0 22.0 37.0 98-99 32.29951419459218 33.0 33.0 37.0 22.0 37.0 100 31.779097752015705 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5599813104033835 1101 2 4.09824722254827 1101 3 2.327127963563658 1101 4 1.0814657408183095 1101 5 0.48066942132399504 1101 6 0.36408461971718253 1101 7 0.4358694608945868 1101 8 0.4468477027093627 1101 9 0.4607623438578301 1101 10-11 0.49114961144736213 1101 12-13 0.5126474564074144 1101 14-15 0.8156524630988002 1101 16-17 0.7908820238807337 1101 18-19 0.8340567937358401 1101 20-21 0.7697515049506052 1101 22-23 0.7265329351057588 1101 24-25 0.7579408242536729 1101 26-27 0.7853365259102318 1101 28-29 0.7848076287794257 1101 30-31 0.6717081445203092 1101 32-33 0.8479594113276008 1101 34-35 0.9097561961706546 1101 36-37 0.7661793392728669 1101 38-39 0.7799506389505737 1101 40-41 0.8020567156993863 1101 42-43 0.9482462586913414 1101 44-45 1.0194516147699133 1101 46-47 0.9890082039689929 1101 48-49 0.902404763324185 1101 50-51 0.9630857543643927 1101 52-53 0.8740551565678842 1101 54-55 0.8482371624980303 1101 56-57 0.8406258371317819 1101 58-59 0.8437921140086075 1101 60-61 0.8330655506712574 1101 62-63 0.7850196974871437 1101 64-65 0.8417035612678134 1101 66-67 0.7355373304063093 1101 68-69 0.6989968268464821 1101 70-71 0.7299049315044712 1101 72-73 0.6266012951719588 1101 74-75 0.6800257538351744 1101 76-77 0.7280156511250837 1101 78-79 0.7361761104824325 1101 80-81 0.6863854355805472 1101 82-83 0.6696825577488354 1101 84-85 0.6867247018625164 1101 86-87 0.6959693721354014 1101 88-89 0.7040761926185581 1101 90-91 0.6732065762878534 1101 92-93 0.6856677490480152 1101 94-95 0.7442447550590359 1101 96-97 0.5982315858872127 1101 98-99 0.6474632575843415 1101 100 0.6354034784933198 1102 1 0.4539239674584117 1102 2 4.026528701898325 1102 3 2.1944841586595665 1102 4 0.9360657348644636 1102 5 0.4515077192069228 1102 6 0.3217490243760466 1102 7 0.3242087198586816 1102 8 0.4339623544970763 1102 9 0.48892505114825724 1102 10-11 0.5156079335838015 1102 12-13 0.5404674362558026 1102 14-15 0.7478415367914835 1102 16-17 0.6611666379080461 1102 18-19 0.755244266388857 1102 20-21 0.7118628239471505 1102 22-23 0.7101877348105106 1102 24-25 0.6802803305191389 1102 26-27 0.6776306609600482 1102 28-29 0.7069279981074104 1102 30-31 0.7191421039412234 1102 32-33 0.7355386117578249 1102 34-35 0.859585331947045 1102 36-37 0.7422300230547307 1102 38-39 0.6260545029582545 1102 40-41 0.6445131051619697 1102 42-43 0.6647812158472988 1102 44-45 0.7477697560264929 1102 46-47 0.7378696106483957 1102 48-49 0.6035780887661346 1102 50-51 0.6120521919095339 1102 52-53 0.6884118877508953 1102 54-55 0.6329498255077084 1102 56-57 0.6359963506720945 1102 58-59 0.6409870368360515 1102 60-61 0.6567272566800071 1102 62-63 0.6954721883824462 1102 64-65 0.6861023738039833 1102 66-67 0.6036876862691329 1102 68-69 0.6710011201972677 1102 70-71 0.6770416157419419 1102 72-73 0.659695726983422 1102 74-75 0.6360841439192981 1102 76-77 0.6582096840046106 1102 78-79 0.5634467153926579 1102 80-81 0.5469447547298643 1102 82-83 0.6550141843122077 1102 84-85 0.6023329689499164 1102 86-87 0.6689778358281941 1102 88-89 0.6395365932259125 1102 90-91 0.6164702699191622 1102 92-93 0.6698707722390651 1102 94-95 0.5917507424081521 1102 96-97 0.6811548766451807 1102 98-99 0.6882849344400839 1102 100 0.684439761423743 1103 1 0.3622858510965159 1103 2 3.9401537644702387 1103 3 2.1702286094201213 1103 4 0.804816905538452 1103 5 0.26997611704616276 1103 6 0.19461187131160784 1103 7 0.20612609784916458 1103 8 0.2463534451051217 1103 9 0.34871877104929183 1103 10-11 0.3631882809778375 1103 12-13 0.3682840330029151 1103 14-15 0.503292574228901 1103 16-17 0.4970384914463679 1103 18-19 0.5074435630707654 1103 20-21 0.5207261680316435 1103 22-23 0.502186479945891 1103 24-25 0.5153384228703857 1103 26-27 0.45350171990850185 1103 28-29 0.41037267980482284 1103 30-31 0.4175290661083011 1103 32-33 0.46857821741281924 1103 34-35 0.6436712183014492 1103 36-37 0.4889979430958249 1103 38-39 0.4335988196990712 1103 40-41 0.44347140518344474 1103 42-43 0.45126808520889483 1103 44-45 0.48507587691380394 1103 46-47 0.44245669884035976 1103 48-49 0.42670562631417397 1103 50-51 0.38401423988252503 1103 52-53 0.3782126292484591 1103 54-55 0.4377169323586756 1103 56-57 0.3430978181670028 1103 58-59 0.3926241244732722 1103 60-61 0.3260037377335223 1103 62-63 0.3678174260969769 1103 64-65 0.31980798690431556 1103 66-67 0.28649762771421905 1103 68-69 0.3269773578811126 1103 70-71 0.3703469554185048 1103 72-73 0.35164834674785794 1103 74-75 0.28520143533701514 1103 76-77 0.2972386617345606 1103 78-79 0.43846454063771034 1103 80-81 0.377293963925311 1103 82-83 0.33843881118128394 1103 84-85 0.3491955626609382 1103 86-87 0.3314803566523281 1103 88-89 0.3577403126896783 1103 90-91 0.3209729839249036 1103 92-93 0.3457110224745037 1103 94-95 0.3346013914918089 1103 96-97 0.30574053147533675 1103 98-99 0.3649416176098512 1103 100 0.41598757354418936 1104 1 0.34062716126362247 1104 2 3.5520309673520245 1104 3 2.083155129855708 1104 4 0.7603467884608932 1104 5 0.3075282910061574 1104 6 0.1843022833171517 1104 7 0.11972141353199106 1104 8 0.1585337192985179 1104 9 0.20534668634523712 1104 10-11 0.3231022023807739 1104 12-13 0.2922937655050646 1104 14-15 0.3571189655457516 1104 16-17 0.3833122937063891 1104 18-19 0.36829091239061285 1104 20-21 0.36638333550533275 1104 22-23 0.31409652778664565 1104 24-25 0.33071385433486 1104 26-27 0.41590875340377664 1104 28-29 0.349448065102429 1104 30-31 0.24176656190670798 1104 32-33 0.3980705185476907 1104 34-35 0.403010264334668 1104 36-37 0.22976448521093573 1104 38-39 0.28226012396707745 1104 40-41 0.26842452117702464 1104 42-43 0.31493800864208765 1104 44-45 0.3919147744570992 1104 46-47 0.43922944749686366 1104 48-49 0.28525593773804303 1104 50-51 0.16429243238216173 1104 52-53 0.22091569090822105 1104 54-55 0.2681605898815107 1104 56-57 0.2664188442728843 1104 58-59 0.3153611464538386 1104 60-61 0.193747726928855 1104 62-63 0.2484116152812419 1104 64-65 0.30421713536858874 1104 66-67 0.1966960254404384 1104 68-69 0.2621572028036425 1104 70-71 0.22232405658468934 1104 72-73 0.19907566599174942 1104 74-75 0.2625984423875849 1104 76-77 0.18440011314257987 1104 78-79 0.19096786141238908 1104 80-81 0.26303016516155964 1104 82-83 0.16299965222822266 1104 84-85 0.26937041629712155 1104 86-87 0.27967711525225525 1104 88-89 0.22644256811665286 1104 90-91 0.18966519940807558 1104 92-93 0.21360147673217966 1104 94-95 0.243940867808341 1104 96-97 0.19069492481975203 1104 98-99 0.27085641847811814 1104 100 0.0534401411374823 1105 1 0.5261309418554561 1105 2 3.706675636870731 1105 3 2.1768241502881587 1105 4 0.9685059006627981 1105 5 0.374750100239865 1105 6 0.37698910776514083 1105 7 0.32588501181044904 1105 8 0.2820927163653124 1105 9 0.2743447045378744 1105 10-11 0.2154266898763595 1105 12-13 0.284802324042559 1105 14-15 0.3689106387297585 1105 16-17 0.45181768199711314 1105 18-19 0.4556883752256766 1105 20-21 0.36167674458488364 1105 22-23 0.42478828982141437 1105 24-25 0.48611399720559234 1105 26-27 0.44492715405782235 1105 28-29 0.36768746549686426 1105 30-31 0.38960431170876575 1105 32-33 0.47556297590819696 1105 34-35 0.5503658657220996 1105 36-37 0.41656877649307944 1105 38-39 0.406709241183961 1105 40-41 0.45982736343386676 1105 42-43 0.5373023580400513 1105 44-45 0.40452763185565743 1105 46-47 0.5202368313015171 1105 48-49 0.3626903437844149 1105 50-51 0.38909130876673004 1105 52-53 0.2909739518521093 1105 54-55 0.3089239001609414 1105 56-57 0.29048998293328765 1105 58-59 0.3400228766087565 1105 60-61 0.38418909429530856 1105 62-63 0.46411659205264755 1105 64-65 0.3792000827950588 1105 66-67 0.3167828282804308 1105 68-69 0.36828145519140065 1105 70-71 0.39535446418555154 1105 72-73 0.2736316943788424 1105 74-75 0.26346518075838077 1105 76-77 0.2627568900691202 1105 78-79 0.3245604637399282 1105 80-81 0.37088426608377034 1105 82-83 0.44456182921700105 1105 84-85 0.3948205155487443 1105 86-87 0.42934106264486616 1105 88-89 0.33538190610590846 1105 90-91 0.37875163297721315 1105 92-93 0.4195034409628988 1105 94-95 0.36300129116367685 1105 96-97 0.29128817699949394 1105 98-99 0.34619694587430416 1105 100 0.40672915121744424 1106 1 0.35815076383884303 1106 2 3.8212390262300424 1106 3 2.1628163183086535 1106 4 0.8234097371007607 1106 5 0.3208977074451411 1106 6 0.1254068526317127 1106 7 0.05571813011965787 1106 8 0.10459604273078327 1106 9 0.0949903953966853 1106 10-11 0.10290047725190732 1106 12-13 0.042206877377687135 1106 14-15 0.10891609043191863 1106 16-17 0.10815176726022457 1106 18-19 0.07465805838310757 1106 20-21 0.09802420222996489 1106 22-23 0.044861468822581685 1106 24-25 0.03323098572123229 1106 26-27 0.07614782949279686 1106 28-29 0.06007893262697195 1106 30-31 -0.002787916065969398 1106 32-33 0.1502611423514537 1106 34-35 0.24292130788295196 1106 36-37 0.15599873610700854 1106 38-39 0.03125933339445908 1106 40-41 0.029401906628315544 1106 42-43 0.051597863324431614 1106 44-45 -0.022872890894241493 1106 46-47 -0.015862386020096153 1106 48-49 -0.12731200091305084 1106 50-51 -0.13623568425417432 1106 52-53 -0.13484492885225308 1106 54-55 -0.06773196188821373 1106 56-57 -0.12757566369091933 1106 58-59 -0.1519733545841575 1106 60-61 -0.012918922354579365 1106 62-63 -0.03213870871558555 1106 64-65 -0.14886340201891812 1106 66-67 -0.16640607332419677 1106 68-69 -0.16810826187414563 1106 70-71 -0.12354437510816041 1106 72-73 -0.10780918351978386 1106 74-75 -0.19359189930312937 1106 76-77 -0.19170601771902085 1106 78-79 -0.17774659635985302 1106 80-81 -0.09979583459232799 1106 82-83 -0.09792856951498408 1106 84-85 -0.07417390518524769 1106 86-87 0.05393644022823452 1106 88-89 -0.018395116366065167 1106 90-91 0.015338323749531924 1106 92-93 0.005709582889792841 1106 94-95 0.10335169312696024 1106 96-97 0.09253506059064875 1106 98-99 0.014532216080425542 1106 100 0.0659958540486798 1107 1 0.3602566735188617 1107 2 3.586345031422187 1107 3 2.0532668138441252 1107 4 0.9152919859071886 1107 5 0.23131529441091914 1107 6 0.075202421864077 1107 7 -0.04379273504773096 1107 8 0.05370665240324257 1107 9 0.02938210587290513 1107 10-11 0.010621670728959032 1107 12-13 -0.04391212260373578 1107 14-15 -0.0025354898957701266 1107 16-17 -0.018903525968632096 1107 18-19 0.011063482808474134 1107 20-21 0.05077030430722118 1107 22-23 0.008856486439263733 1107 24-25 0.027640894076021993 1107 26-27 -0.08305108661041771 1107 28-29 -0.006268705305139122 1107 30-31 4.6654348795982514E-4 1107 32-33 0.05690299493512185 1107 34-35 0.2862558715981578 1107 36-37 0.025299597307991917 1107 38-39 -0.005886115154353888 1107 40-41 0.07868282552791328 1107 42-43 0.12292253689184207 1107 44-45 0.15037567625807213 1107 46-47 0.1589832524585475 1107 48-49 -0.026925670124050782 1107 50-51 -0.07043371736769899 1107 52-53 -0.027426621565872722 1107 54-55 0.044832226902109085 1107 56-57 0.10308735958617632 1107 58-59 0.0983118759582311 1107 60-61 0.1678127129522764 1107 62-63 0.2555876061016562 1107 64-65 0.11625819022125228 1107 66-67 0.1778348357787749 1107 68-69 0.09539497194085556 1107 70-71 0.11587311783787158 1107 72-73 0.12221555843165532 1107 74-75 0.09865781001500551 1107 76-77 0.1494579832307963 1107 78-79 0.114304087911556 1107 80-81 0.2022863794582932 1107 82-83 0.04580146579797173 1107 84-85 0.20765909601131227 1107 86-87 0.13663207260472632 1107 88-89 0.22831504231008637 1107 90-91 0.1090556572765422 1107 92-93 0.1494759119807867 1107 94-95 0.0552764489740909 1107 96-97 0.085961305995788 1107 98-99 0.1380077115827305 1107 100 0.11481038442750346 1108 1 0.4764434615956681 1108 2 3.7037852460259515 1108 3 2.1525644829487547 1108 4 1.046862627411052 1108 5 0.28100537419510374 1108 6 0.053211955816856005 1108 7 0.06527877668519722 1108 8 0.11455676072039012 1108 9 0.03615893233208567 1108 10-11 0.06717980268997081 1108 12-13 0.004405389206986854 1108 14-15 0.04267550604184578 1108 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0.8501418322628282 2202 58-59 0.885850671062073 2202 60-61 0.897755644266816 2202 62-63 0.9528568417051702 2202 64-65 0.8221453774432845 2202 66-67 0.8459041690347533 2202 68-69 0.8509507692104563 2202 70-71 0.8149083162367603 2202 72-73 0.814356226335903 2202 74-75 0.8211996990585888 2202 76-77 0.7952096172177292 2202 78-79 0.8108653430842381 2202 80-81 0.685667572172072 2202 82-83 0.8390414491680431 2202 84-85 0.8378183613939925 2202 86-87 0.8813619813335052 2202 88-89 0.880006678037347 2202 90-91 0.9389520586378808 2202 92-93 0.8924097645158895 2202 94-95 0.9202749261756153 2202 96-97 0.832443883247592 2202 98-99 0.8783761321264905 2202 100 0.7705614866435404 2203 1 0.3182535403269533 2203 2 3.901905216444593 2203 3 2.198259713374931 2203 4 0.8421276085802489 2203 5 0.43156610603264056 2203 6 0.40429342018808967 2203 7 0.4029591177156888 2203 8 0.3719170606948694 2203 9 0.46833345349703137 2203 10-11 0.4423160360622589 2203 12-13 0.4421660865739341 2203 14-15 0.6920958668310249 2203 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0.6260647252160965 2203 84-85 0.5868361765366785 2203 86-87 0.6248973752390228 2203 88-89 0.577552218003234 2203 90-91 0.5912030315679573 2203 92-93 0.6915167084248992 2203 94-95 0.6679971910366049 2203 96-97 0.5855296130954741 2203 98-99 0.629098063344081 2203 100 0.5214031993777475 2204 1 0.4161798511589154 2204 2 3.9622237947201846 2204 3 2.2841828488268305 2204 4 0.9467497306788957 2204 5 0.460527664094883 2204 6 0.4116469319077467 2204 7 0.35177245726789863 2204 8 0.467224223007328 2204 9 0.38439024084348716 2204 10-11 0.43491832709954537 2204 12-13 0.4829217713199725 2204 14-15 0.6121909463277859 2204 16-17 0.5786596374327289 2204 18-19 0.6839148749107267 2204 20-21 0.637542456701226 2204 22-23 0.5108639019827521 2204 24-25 0.6327111110596277 2204 26-27 0.6135942827831755 2204 28-29 0.5658918220061224 2204 30-31 0.5452650739319935 2204 32-33 0.7334955619429095 2204 34-35 0.7904000280719714 2204 36-37 0.6003142322632584 2204 38-39 0.5648960582713656 2204 40-41 0.5922126876629719 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0.3870489591107287 2210 48-49 0.34310750962205105 2210 50-51 0.32629585590888865 2210 52-53 0.19370501815699015 2210 54-55 0.2483409591093917 2210 56-57 0.21347770510082853 2210 58-59 0.2577437636093691 2210 60-61 0.3218770667538422 2210 62-63 0.2934997349639872 2210 64-65 0.02694646476124518 2210 66-67 0.19630445418810183 2210 68-69 0.27274057338389923 2210 70-71 0.31107913038145796 2210 72-73 0.37801517427492115 2210 74-75 -0.15944113298790086 2210 76-77 0.20439937857261015 2210 78-79 0.23166411410063148 2210 80-81 0.23554725886780403 2210 82-83 0.3099757363529889 2210 84-85 0.17790227892727728 2210 86-87 0.2841545823784557 2210 88-89 0.394826282102386 2210 90-91 0.4897014972137441 2210 92-93 0.606919845096769 2210 94-95 0.453169483208562 2210 96-97 0.3904583550078513 2210 98-99 0.3345026586762714 2210 100 0.33039335549121773 2211 1 0.33814015796806984 2211 2 3.8922108477563135 2211 3 2.1272725171125266 2211 4 0.8713305326850502 2211 5 0.36070281251610936 2211 6 0.04599663362623119 2211 7 0.06794288554826977 2211 8 0.01816836723344295 2211 9 -0.061214174400781474 2211 10-11 0.055508456118893434 2211 12-13 -0.1493628687716111 2211 14-15 -0.15349430736353042 2211 16-17 -0.20175632730176574 2211 18-19 -0.0956248835023672 2211 20-21 -0.11330995858760673 2211 22-23 -0.14016157275860763 2211 24-25 -0.1540745834969357 2211 26-27 -0.1711344739137033 2211 28-29 -0.20807572841810185 2211 30-31 -0.197844462767236 2211 32-33 -0.1689769878476639 2211 34-35 -0.11738848287863846 2211 36-37 -0.2678398110749498 2211 38-39 -0.29843919846057076 2211 40-41 -0.2415063311902017 2211 42-43 -0.11753195925740556 2211 44-45 -0.13085162578651222 2211 46-47 -0.1518697333827035 2211 48-49 -0.2660836577306398 2211 50-51 -0.30932428118623534 2211 52-53 -0.2886534357539645 2211 54-55 -0.29703553766764657 2211 56-57 -0.30785862473009473 2211 58-59 -0.3808493073077841 2211 60-61 -0.37380081457718717 2211 62-63 -0.3468967864784531 2211 64-65 -0.3121327181542455 2211 66-67 -0.2740389186584693 2211 68-69 -0.2934366901848904 2211 70-71 -0.3831977946957039 2211 72-73 -0.3472735375104392 2211 74-75 -0.2996311230601165 2211 76-77 -0.40024542128970353 2211 78-79 -0.43597068695662244 2211 80-81 -0.22140821227360874 2211 82-83 -0.6185401725278297 2211 84-85 -0.38828841833991135 2211 86-87 -0.1997405269286361 2211 88-89 -0.10067779723271997 2211 90-91 -0.20116251693315235 2211 92-93 -0.1265513981459918 2211 94-95 -0.17326501032033548 2211 96-97 -0.22923918574229063 2211 98-99 -0.2030150775633004 2211 100 -0.23413668300995027 2212 1 0.6624392057721629 2212 2 4.0959029708721 2212 3 2.394099481517607 2212 4 1.3189442223162828 2212 5 0.7187031203242213 2212 6 0.655728672784555 2212 7 0.5638876275704732 2212 8 0.557228409079606 2212 9 0.3614894289633952 2212 10-11 0.4794152246453294 2212 12-13 0.44663960280741577 2212 14-15 0.49452949618778064 2212 16-17 0.48997198211706205 2212 18-19 0.47050321562925035 2212 20-21 0.4263611424353826 2212 22-23 0.4598118513456697 2212 24-25 0.5038853529777612 2212 26-27 0.4063244230354073 2212 28-29 0.4019005272722538 2212 30-31 0.45473753153520136 2212 32-33 0.5209623681539668 2212 34-35 0.5647047697230789 2212 36-37 0.423777368429171 2212 38-39 0.4705321693777904 2212 40-41 0.4930288485223002 2212 42-43 0.47519285303867065 2212 44-45 0.4620976212953778 2212 46-47 0.380012227383979 2212 48-49 0.4475815725672092 2212 50-51 0.4047088274499302 2212 52-53 0.49978534533419605 2212 54-55 0.44882795419790966 2212 56-57 0.4310904736261918 2212 58-59 0.5631683508524645 2212 60-61 0.5586229796114495 2212 62-63 0.561610358667572 2212 64-65 0.6387080889161396 2212 66-67 0.46548271107571537 2212 68-69 0.4921104345952614 2212 70-71 0.4295465383518504 2212 72-73 0.4728054734768179 2212 74-75 0.7130313694854706 2212 76-77 0.6728247243681622 2212 78-79 0.4828719545341258 2212 80-81 0.532236548577508 2212 82-83 0.4711205404891601 2212 84-85 0.6569872345975298 2212 86-87 0.657116064525276 2212 88-89 0.6279098088714932 2212 90-91 0.8068776754324105 2212 92-93 0.7607577934475742 2212 94-95 0.6163764180833127 2212 96-97 0.5023273856336701 2212 98-99 0.6407596690387507 2212 100 0.6363204286106772 2213 1 -0.6105358101412683 2213 2 -25.91937392282618 2213 3 -14.783635673139367 2213 4 -6.182761077125935 2213 5 -2.178413851484727 2213 6 -1.3778550723200382 2213 7 -1.3589652729894652 2213 8 -1.3212068016789544 2213 9 -1.1238803290445674 2213 10-11 -1.175422626933603 2213 12-13 -1.2136754022093683 2213 14-15 -1.3910210748076963 2213 16-17 -1.5076490948176442 2213 18-19 -1.4185781478550865 2213 20-21 -1.5596969051754428 2213 22-23 -1.560021947702758 2213 24-25 -1.5836986982580896 2213 26-27 -1.5530767734388249 2213 28-29 -1.5200329680723925 2213 30-31 -1.6533411307116666 2213 32-33 -1.5198736196655247 2213 34-35 -1.5727729447306302 2213 36-37 -1.5528357882021453 2213 38-39 -1.6605471321035026 2213 40-41 -1.6330240676813474 2213 42-43 -1.4253648924866837 2213 44-45 -1.2343745868415184 2213 46-47 -1.3414165896816925 2213 48-49 -1.6326155449440378 2213 50-51 -1.5538515195168046 2213 52-53 -1.5586766215658727 2213 54-55 -1.4783343843751666 2213 56-57 -1.508987873049243 2213 58-59 -1.5277007112800476 2213 60-61 -1.575010899543571 2213 62-63 -1.459878841776927 2213 64-65 -1.427852533164149 2213 66-67 -1.5916604976653446 2213 68-69 -1.4552638155854467 2213 70-71 -1.489207473720782 2213 72-73 -1.4670773268015296 2213 74-75 -1.5169831144338701 2213 76-77 -1.5806554957766252 2213 78-79 -1.402335230245086 2213 80-81 -1.6003110366267776 2213 82-83 -1.542499603217209 2213 84-85 -1.3811647103473916 2213 86-87 -1.4366290468147938 2213 88-89 -1.3164638155607484 2213 90-91 -1.4025051464234721 2213 92-93 -1.3441629630607501 2213 94-95 -1.4808962828305 2213 96-97 -1.6624202853413124 2213 98-99 -1.5592789008023331 2213 100 -1.489470522850663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 21.0 5 27.0 6 22.0 7 30.0 8 37.0 9 72.0 10 282.0 11 1245.0 12 3462.0 13 6331.0 14 8661.0 15 10170.0 16 11062.0 17 11730.0 18 12386.0 19 13312.0 20 15740.0 21 18589.0 22 21092.0 23 22312.0 24 21801.0 25 21576.0 26 23045.0 27 26537.0 28 30503.0 29 36950.0 30 45188.0 31 55622.0 32 70310.0 33 95083.0 34 131724.0 35 187984.0 36 290146.0 37 507566.0 38 553146.0 39 5359.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.83062242618458 21.48564908323996 10.997998044435562 28.6857304461399 2 12.851302142639694 23.14208929231958 37.53591992656319 26.47068863847754 3 15.401248708023205 28.2919790096652 28.63463228182468 27.672140000486912 4 10.321364589913427 18.786085843304825 38.26897734108949 32.62357222569226 5 11.543736169016558 39.00697584812666 34.77437899965105 14.674908983205734 6 29.106110686182763 40.235980649250166 17.8563946893946 12.801513975172474 7 25.232758017565004 33.81223669982651 23.469349800784112 17.485655481824374 8 23.853279668196055 37.25957078222886 21.03470860211292 17.85244094746216 9 24.325871803387578 17.088940551311858 22.790036532713383 35.795151112587185 10-11 22.691242517290274 28.484425247298077 28.903740402779903 19.920591832631743 12-13 23.374246508615677 26.216285930952786 29.801449138985603 20.608018421445927 14-15 21.11403848082723 27.346885635594354 27.26529372313528 24.273782160443133 16-17 19.820863178584876 31.282436058857087 27.82844204410629 21.068258718451744 18-19 19.599151830564473 29.730273618326407 30.717671952892857 19.95290259821626 20-21 21.775184370369157 28.774218742044415 29.520245210266566 19.930351677319866 22-23 20.46794202426289 28.984415141095205 30.23087622189425 20.316766612747656 24-25 20.430623810156284 28.71184752291141 30.044826670208526 20.812701996723778 26-27 20.31477368461362 28.855007014597177 30.056740985833123 20.77347831495608 28-29 19.89370537852821 28.821962153819726 30.46632122058403 20.818011247068032 30-31 20.816033681108458 29.229691638011325 29.565608361479512 20.38866631940071 32-33 19.72325354149814 29.201731214650035 30.566274513275708 20.508740730576125 34-35 20.035386274339682 28.497282950314023 30.536575260240063 20.93075551510623 36-37 20.513828988087518 29.28781408542229 29.713964973911704 20.484391952578484 38-39 20.182912185993185 29.157627852054496 30.12840816154882 20.531051800403496 40-41 20.633847676645875 28.905284070762377 29.77586955626767 20.68499869632408 42-43 19.95454136983245 29.078759987374813 30.15973602210204 20.806962620690694 44-45 20.569274863692897 28.56451958123235 29.898716733330783 20.967488821743974 46-47 20.703576364877584 29.3895272391129 29.34746902523227 20.559427370777243 48-49 20.423354309630692 29.10694812362275 29.56458059285897 20.905116973887587 50-51 21.042123473789186 28.98299100239855 28.923929369548063 21.050956154264203 52-53 20.437511498109274 29.33369964935056 29.28707626907579 20.941712583464376 54-55 20.863291992799958 29.066166474475214 28.990389394130915 21.08015213859391 56-57 20.82023361565932 29.08131789708276 29.00734041660699 21.09110807065093 58-59 20.272969293112723 28.658566144219638 29.688681130968288 21.37978343169935 60-61 21.01145722912621 29.08461916627248 28.725060367319283 21.178863237282027 62-63 20.266264199325818 29.389715654875697 29.292825095828018 21.05119504997047 64-65 20.404272679739837 28.498204516445796 29.454142894015778 21.64337990979859 66-67 20.489445906201674 29.044599607649896 29.06368140569541 21.40227308045302 68-69 20.708619267462552 28.95299736598934 29.208583624857376 21.129799741690736 70-71 21.05443057870816 28.794677151199988 28.767934507087467 21.382957763004384 72-73 20.427570060355333 29.17860608937123 29.33956692216146 21.054256928111982 74-75 20.88839310619028 28.50931061017282 29.02431853984272 21.57797774379418 76-77 20.821086455761378 29.161248820691544 28.832437036771402 21.185227686775672 78-79 20.743220733857427 29.0267584698631 29.12056861610112 21.109452180178344 80-81 21.124518366966576 28.67837925600687 28.78150688524677 21.415595491779783 82-83 20.378573110764442 29.150877662070446 29.312500996035336 21.15804823112978 84-85 20.640715357982238 28.425709538770473 29.12304102400419 21.810534079243098 86-87 20.76775781931493 29.06396646393897 28.815929938867384 21.35234577787871 88-89 20.36375644980221 28.660163152992673 29.22880374540059 21.74727665180453 90-91 20.74664982133561 28.58273896583597 28.921585987328818 21.749025225499604 92-93 20.231982008402188 29.119239929802493 29.108638149278825 21.540139912516498 94-95 20.24367215100166 28.352340156549 29.255863283669036 22.148124408780305 96-97 20.31522127172563 28.794749617272096 29.094270026069985 21.795759084932286 98-99 20.240443394280422 28.519780728585225 29.202325863456448 22.037450013677905 100 20.57991936480844 28.354715085001935 28.810353987516123 22.255011562673502 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8.0 1 9.0 2 11.0 3 14.5 4 16.0 5 17.5 6 31.5 7 53.0 8 88.5 9 134.0 10 160.5 11 180.0 12 217.0 13 276.0 14 374.5 15 482.0 16 594.5 17 762.0 18 915.5 19 1198.5 20 1647.0 21 2301.0 22 3301.5 23 4673.0 24 6719.0 25 9620.0 26 13717.5 27 19356.0 28 26279.0 29 34376.5 30 43558.5 31 52803.5 32 61607.0 33 70331.0 34 78265.5 35 84928.5 36 90724.0 37 95712.5 38 99599.0 39 101672.5 40 101682.0 41 100099.0 42 96594.0 43 92209.0 44 87469.5 45 81972.5 46 76767.5 47 72109.0 48 68121.0 49 64907.5 50 63522.5 51 59415.5 52 51874.0 53 46495.0 54 42874.5 55 39347.5 56 35795.0 57 32601.5 58 28864.0 59 24799.5 60 20541.0 61 16253.5 62 12853.0 63 9842.5 64 7262.0 65 5338.0 66 3851.0 67 2804.0 68 1962.5 69 1337.0 70 941.5 71 617.5 72 422.5 73 311.5 74 219.0 75 144.0 76 85.0 77 43.0 78 22.0 79 11.0 80 8.0 81 5.0 82 2.5 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7669749705086238 2 5.342664338341888 3 0.0 4 0.004957649719914923 5 0.16740920750641286 6 0.054799735296916736 7 0.03457075385047817 8 0.04134325748571909 9 0.03917428573325631 10-11 0.041121933837508604 12-13 0.03972759485378253 14-15 0.041852301876603215 16-17 0.033176414866752095 18-19 0.02762119129666886 20-21 0.02222089428033296 22-23 0.02215449718586981 24-25 0.019255157394312425 26-27 0.012128535921934722 28-29 0.01828133334218628 30-31 0.023880821641911614 32-33 0.001217280065157682 34-35 0.0029657368860205344 36-37 0.003364119452799412 38-39 0.005643753029367435 40-41 0.006108532690609459 42-43 0.006993827283451409 44-45 0.007679930592903922 46-47 0.015979567400797208 48-49 0.024522660221722028 50-51 0.02113640840410157 52-53 0.027067882176142637 54-55 0.023748027452985324 56-57 0.01502787571349211 58-59 0.013699933824229184 60-61 0.011309638423555918 62-63 0.01775015658648111 64-65 0.01823706861254418 66-67 0.019387951583238718 68-69 0.02664736724454271 70-71 0.02523089589599559 72-73 0.022619276847111836 74-75 0.023416041980669593 76-77 0.01752883293827062 78-79 0.014054051661365965 80-81 0.01254905085353465 82-83 0.007923386605935457 84-85 0.010535005654819211 86-87 0.008565225185745871 88-89 0.006683974175956726 90-91 0.006683974175956726 92-93 0.0035633107361888513 94-95 0.001505000807831316 96-97 0.001040221146589292 98-99 7.52500403915658E-4 100 0.10198593709539269 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2259135.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.78919575712376 #Duplication Level Percentage of deduplicated Percentage of total 1 93.35065296496558 84.75230706091585 2 5.605799014276294 10.178919681644437 3 0.658340452421721 1.7931060062924713 4 0.16386961037967618 0.5951036054161608 5 0.0733092324169904 0.3327843126355312 6 0.03537045073390164 0.19267528654187388 7 0.022842967410577644 0.14517262479387727 8 0.018684586224647942 0.1357086845112336 9 0.011936619314694155 0.09753444608760291 >10 0.05439071536193088 0.8147722866208313 >50 0.0021624518319200686 0.1338548506556907 >100 0.002152730712374697 0.3839825650683816 >500 2.9292236982165256E-4 0.14653396692808565 >1k 1.9528157987555822E-4 0.29754462188808134 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2132364821048765E-5 0.0 12-13 0.0 0.0 0.0 4.426472964209753E-5 0.0 14-15 0.0 0.0 0.0 1.1066182410524383E-4 0.0 16-17 0.0 0.0 0.0 1.327941889262926E-4 0.0 18-19 0.0 0.0 0.0 1.327941889262926E-4 0.0 20-21 0.0 0.0 0.0 1.327941889262926E-4 0.0 22-23 0.0 2.2132364821048765E-5 0.0 2.655883778525852E-4 0.0 24-25 0.0 4.426472964209753E-5 0.0 3.76250201957829E-4 0.0 26-27 0.0 4.426472964209753E-5 0.0 4.8691202606307286E-4 0.0 28-29 0.0 4.426472964209753E-5 0.0 7.746327687367068E-4 0.0 30-31 0.0 4.426472964209753E-5 0.0 0.0024566924951364127 0.0 32-33 0.0 4.426472964209753E-5 0.0 0.008543092820924823 0.0 34-35 0.0 4.426472964209753E-5 0.0 0.01688699435846021 0.0 36-37 0.0 4.426472964209753E-5 0.0 0.029502442306458 0.0 38-39 0.0 4.426472964209753E-5 0.0 0.05001914449557021 0.0 40-41 0.0 4.426472964209753E-5 0.0 0.07376717194855553 0.0 42-43 0.0 4.426472964209753E-5 0.0 0.09846689108884596 0.0 44-45 0.0 4.426472964209753E-5 0.0 0.12931940764938793 0.0 46-47 0.0 4.426472964209753E-5 0.0 0.1648861179168133 0.0 48-49 0.0 4.426472964209753E-5 0.0 0.2058752575653956 0.0 50-51 0.0 8.852945928419506E-5 0.0 0.2473955739696831 0.0 52-53 0.0 8.852945928419506E-5 0.0 0.2912619210450017 0.0 54-55 0.0 8.852945928419506E-5 0.0 0.34203356594448764 0.0 56-57 0.0 8.852945928419506E-5 0.0 0.4029860986616559 0.0 58-59 0.0 8.852945928419506E-5 0.0 0.4741859162909698 0.0 60-61 0.0 8.852945928419506E-5 0.0 0.5512065458682195 0.0 62-63 0.0 1.1066182410524383E-4 0.0 0.6334061488135946 0.0 64-65 0.0 1.327941889262926E-4 0.0 0.7196338421564006 0.0 66-67 0.0 1.327941889262926E-4 0.0 0.8238994128283613 0.0 68-69 0.0 1.327941889262926E-4 0.0 0.9413780052984881 0.0 70-71 0.0 1.327941889262926E-4 0.0 1.0760091805049279 0.0 72-73 0.0 1.327941889262926E-4 0.0 1.2170366091446505 0.0 74-75 0.0 1.327941889262926E-4 0.0 1.3633094082469617 0.0 76-77 0.0 1.327941889262926E-4 0.0 1.5271553050171858 0.0 78-79 0.0 1.327941889262926E-4 0.0 1.7136647433641636 0.0 80-81 0.0 1.327941889262926E-4 0.0 1.9256042688905266 0.0 82-83 0.0 1.327941889262926E-4 0.0 2.1555374070164026 0.0 84-85 0.0 1.327941889262926E-4 0.0 2.3903839301325505 0.0 86-87 0.0 1.327941889262926E-4 0.0 2.6477390682717057 0.0 88 0.0 1.327941889262926E-4 0.0 2.8603868294723425 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6840 0.0 36.630405 1 TATCAAC 8190 0.0 30.50241 2 CAACGCA 8285 0.0 29.791927 5 GGTATCA 4285 0.0 29.699999 1 TCAACGC 8330 0.0 29.687428 4 ATCAACG 8360 0.0 29.635818 3 AACGCAG 8530 0.0 28.680164 6 ACGCAGA 9695 0.0 25.08518 7 CGCAGAG 9735 0.0 24.982105 8 GCAGAGT 10830 0.0 22.196104 9 CTGTCGC 330 1.8189894E-12 21.34089 9 GTATAGG 530 0.0 20.635458 1 GAGTACT 4350 0.0 20.236156 12-13 GTACATG 9880 0.0 20.07628 1 CCGTACT 120 0.005056588 19.589375 4 AGTACCG 170 8.611674E-5 19.358912 5 TACATGG 10110 0.0 19.355087 2 ACATGGG 10085 0.0 18.693087 3 CATGGGT 2500 0.0 18.617743 4 TACCGCA 155 0.0010411631 18.17418 7 >>END_MODULE