FastQCFastQC Report
Wed 25 May 2016
SRR1295143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586187
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45810.17713336274600408No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37290.14418910929488085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA10200.018.6658551
GTATATA15100.018.3029861
GTATAGG6750.018.1975351
GTACTAA5900.017.6963331
CATGGGG44950.017.4551074
GTATCGT2000.001839614816.6937664
GTCTAGG6351.8189894E-1216.4422631
GTACTAG6001.2732926E-1116.377781
GTACATG168700.015.2540541
GTGTAGG7401.8189894E-1214.9391931
TATACTG11900.014.6731645
TACATGG168950.014.6469832
CCTAGTA5101.0885378E-714.4481562
CCTAGAC7205.456968E-1213.9125293
TATCGTT2200.00724221613.5862717
ACATGGG172450.013.5212523
TACATCG4104.3358596E-513.4790722
GTATTAT12850.013.3826241
CTAGTAC5053.2062053E-713.2237893
CCTATAC7258.731149E-1113.0489913
Produced by FastQC (version 0.11.5)