##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295143_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2586187
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.826735267016655	33.0	27.0	33.0	15.0	33.0
2	22.916883427223166	27.0	15.0	33.0	2.0	33.0
3	25.23659735355564	33.0	15.0	33.0	15.0	33.0
4	25.5364855673623	33.0	15.0	37.0	2.0	37.0
5	27.32380682448717	33.0	15.0	37.0	2.0	37.0
6	28.174201246854924	33.0	15.0	37.0	6.0	37.0
7	27.82316437287791	33.0	15.0	37.0	6.0	37.0
8	28.678731661708916	33.0	27.0	37.0	6.0	37.0
9	29.551224254085263	33.0	27.0	37.0	15.0	37.0
10-11	29.103021939248787	35.0	24.5	37.0	10.5	37.0
12-13	27.898604393263135	33.0	15.0	37.0	4.0	37.0
14-15	29.710070076139118	33.0	27.0	37.0	10.5	40.0
16-17	30.142081566414184	35.0	27.0	38.5	6.0	40.0
18-19	30.29049407486775	37.0	27.0	40.0	10.5	40.0
20-21	29.839000428043292	33.0	27.0	40.0	6.0	40.0
22-23	30.54598004707316	37.0	27.0	40.0	15.0	40.0
24-25	30.68331872366538	37.0	27.0	40.0	15.0	40.0
26-27	29.286710125756567	33.0	24.5	38.5	4.0	40.0
28-29	29.95722331757139	33.0	27.0	37.0	10.5	40.0
30-31	30.644080455125632	37.0	27.0	40.0	15.0	40.0
32-33	29.452247652625275	33.0	27.0	37.0	6.0	40.0
34-35	29.108131198556023	33.0	22.0	37.0	6.0	40.0
36-37	29.401217120030374	33.0	24.5	37.0	6.0	40.0
38-39	29.490408272874312	33.0	24.5	37.0	6.0	40.0
40-41	28.61097225374654	33.0	22.0	37.0	6.0	40.0
42-43	29.173937344824637	33.0	22.0	37.0	6.0	40.0
44-45	29.0333554766148	33.0	22.0	37.0	6.0	40.0
46-47	28.66315950857382	33.0	22.0	37.0	6.0	40.0
48-49	28.511029944857043	33.0	22.0	37.0	6.0	40.0
50-51	28.479212060071447	33.0	22.0	37.0	6.0	40.0
52-53	28.697472572555657	33.0	22.0	37.0	6.0	40.0
54-55	28.672175097933753	33.0	22.0	37.0	6.0	40.0
56-57	28.674332134528555	33.0	22.0	37.0	6.0	40.0
58-59	28.454393089130832	33.0	22.0	37.0	6.0	40.0
60-61	28.4258131759227	33.0	22.0	37.0	10.5	40.0
62-63	28.328030223645854	33.0	22.0	37.0	6.0	40.0
64-65	27.943372424345185	33.0	22.0	37.0	6.0	40.0
66-67	28.119071242721425	33.0	22.0	37.0	10.5	38.5
68-69	27.69583173993219	33.0	22.0	37.0	6.0	37.0
70-71	27.778019918899908	33.0	22.0	37.0	10.5	37.0
72-73	27.760344669584992	33.0	22.0	37.0	10.5	37.0
74-75	27.848380453540287	33.0	22.0	37.0	15.0	37.0
76-77	27.602785877432684	33.0	22.0	37.0	10.5	37.0
78-79	27.19444146923637	30.0	22.0	33.0	10.5	37.0
80-81	27.03225134145365	27.0	22.0	33.0	10.5	37.0
82-83	27.080823428468243	27.0	22.0	33.0	15.0	37.0
84-85	27.035184617353657	27.0	22.0	33.0	10.5	37.0
86-87	26.80485807870815	27.0	22.0	33.0	15.0	37.0
88-89	26.725690176309755	27.0	22.0	33.0	15.0	37.0
90-91	26.85528057329188	27.0	22.0	33.0	15.0	37.0
92-93	26.760846566779584	27.0	22.0	33.0	15.0	37.0
94-95	26.654550502341863	27.0	22.0	33.0	15.0	37.0
96-97	26.763144737793517	27.0	22.0	33.0	15.0	37.0
98-99	26.83036744829357	27.0	22.0	33.0	15.0	37.0
100	26.465896317629003	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.248778608632307
1101	2	11.199118403953484
1101	3	8.512595850021711
1101	4	12.906140526337094
1101	5	10.694300490803453
1101	6	9.856366254414368
1101	7	9.324040615250734
1101	8	9.023358893283074
1101	9	7.3290640768974455
1101	10-11	7.638181358260489
1101	12-13	9.076550306564751
1101	14-15	7.996197297570479
1101	16-17	8.161910607782865
1101	18-19	8.187704561726203
1101	20-21	7.083602207963562
1101	22-23	7.23145073607683
1101	24-25	7.292468017382134
1101	26-27	8.494623633313903
1101	28-29	8.215547776037127
1101	30-31	5.449305641508701
1101	32-33	6.86458272560305
1101	34-35	8.640985973417848
1101	36-37	8.175469657046161
1101	38-39	8.103391831373855
1101	40-41	8.995028049783308
1101	42-43	8.573992924515782
1101	44-45	8.509099316696897
1101	46-47	7.321970380765226
1101	48-49	5.402524903993456
1101	50-51	6.688888716789833
1101	52-53	5.782227370624099
1101	54-55	3.8411930099834173
1101	56-57	6.061978374534373
1101	58-59	6.259215384660429
1101	60-61	6.793240330720181
1101	62-63	6.213501752153213
1101	64-65	6.675269036656324
1101	66-67	5.7662302572619275
1101	68-69	6.778008971751888
1101	70-71	6.295901450913753
1101	72-73	5.4497613741685385
1101	74-75	4.895315491057264
1101	76-77	4.989721046345011
1101	78-79	5.009546885315313
1101	80-81	4.873265613037333
1101	82-83	4.477362354366157
1101	84-85	4.123600183648765
1101	86-87	3.9440872383434957
1101	88-89	3.9371487617194525
1101	90-91	3.813295005221498
1101	92-93	3.796496448882202
1101	94-95	3.7659145955254623
1101	96-97	3.7882824054286495
1101	98-99	3.4706197046512024
1101	100	3.7282010324509507
1102	1	6.810396600453128
1102	2	10.592105561239059
1102	3	8.281126115183174
1102	4	12.628575406573969
1102	5	10.663113498343076
1102	6	9.55745862129897
1102	7	6.908436404723911
1102	8	7.984705450263625
1102	9	6.528993091686111
1102	10-11	7.5562672647478095
1102	12-13	9.14227922059828
1102	14-15	6.253436219134574
1102	16-17	7.409176814250117
1102	18-19	7.440666468506667
1102	20-21	7.037495857064233
1102	22-23	6.573780767812842
1102	24-25	6.999068344695516
1102	26-27	8.452402847854092
1102	28-29	7.7198849688542595
1102	30-31	4.150569733759994
1102	32-33	4.420769893339255
1102	34-35	4.7905324614505815
1102	36-37	5.083249959195324
1102	38-39	4.5006469849775
1102	40-41	6.26219466902063
1102	42-43	6.904053933087031
1102	44-45	6.44295279677295
1102	46-47	5.358888847784275
1102	48-49	3.6701058333364465
1102	50-51	5.146029028473997
1102	52-53	5.940219022461228
1102	54-55	5.499171763317474
1102	56-57	6.295841178685119
1102	58-59	6.079368709960434
1102	60-61	6.151023667987481
1102	62-63	4.385507185643888
1102	64-65	6.115113549321521
1102	66-67	3.965762144968192
1102	68-69	6.190610602518166
1102	70-71	5.393061275441379
1102	72-73	5.135982381224935
1102	74-75	4.203883398874524
1102	76-77	3.458575011500656
1102	78-79	4.421871370545194
1102	80-81	4.494221872400729
1102	82-83	4.003331190743804
1102	84-85	3.216360869586662
1102	86-87	3.3584339721578793
1102	88-89	3.4281590743498036
1102	90-91	3.383304634306093
1102	92-93	3.2905005025776077
1102	94-95	3.14790557670036
1102	96-97	3.437337102452247
1102	98-99	3.2133732027821615
1102	100	3.3246896254702527
1103	1	6.748637126562365
1103	2	11.029399296397017
1103	3	8.082198656997058
1103	4	12.115874944624363
1103	5	10.47570510454987
1103	6	9.123196136331295
1103	7	8.071793885101247
1103	8	8.046037687351543
1103	9	7.080465424189946
1103	10-11	7.4799017229935245
1103	12-13	8.765847863526535
1103	14-15	8.545452081850012
1103	16-17	8.113289804161695
1103	18-19	7.340188447938164
1103	20-21	8.454138552588038
1103	22-23	6.894748820045393
1103	24-25	5.228229810439103
1103	26-27	6.010818954666181
1103	28-29	7.159352251642339
1103	30-31	6.305047266479605
1103	32-33	8.036496605212513
1103	34-35	7.301025933027454
1103	36-37	4.149530599743624
1103	38-39	6.434043110711876
1103	40-41	6.908876690749587
1103	42-43	7.5778899549708605
1103	44-45	5.917857208957777
1103	46-47	6.524757762811912
1103	48-49	5.623555565250193
1103	50-51	6.592410748212679
1103	52-53	6.253930399322471
1103	54-55	5.085244941389149
1103	56-57	6.015696536286288
1103	58-59	5.638945518268727
1103	60-61	5.177401565874167
1103	62-63	2.9362625826260036
1103	64-65	5.1689597015118025
1103	66-67	4.2154857464664985
1103	68-69	5.2598530399839305
1103	70-71	3.87408873859086
1103	72-73	4.717414798316302
1103	74-75	3.379606206480563
1103	76-77	3.7260421944064106
1103	78-79	2.9427325689867097
1103	80-81	4.304395114725523
1103	82-83	3.600359919151405
1103	84-85	3.4483122134867337
1103	86-87	3.099984136610068
1103	88-89	3.1947036527107784
1103	90-91	2.8519889731327694
1103	92-93	2.57369104846957
1103	94-95	3.1170791482554954
1103	96-97	3.2745531920186473
1103	98-99	3.0082466901938254
1103	100	3.0793221449286996
1104	1	6.624142665510842
1104	2	9.91244286263947
1104	3	7.6660194533643455
1104	4	12.458634629300018
1104	5	9.737839002169533
1104	6	8.33293026491016
1104	7	3.944407161972272
1104	8	6.823530214167089
1104	9	6.859091122221201
1104	10-11	7.273105489560571
1104	12-13	7.486225830346829
1104	14-15	7.299233075953222
1104	16-17	7.3436356368412845
1104	18-19	7.111744603446066
1104	20-21	7.696896201059662
1104	22-23	6.710898427689401
1104	24-25	6.26552288712444
1104	26-27	8.53989885247083
1104	28-29	7.43860892036836
1104	30-31	6.4657549531340095
1104	32-33	7.738160846849638
1104	34-35	8.16546211444723
1104	36-37	6.917762754783038
1104	38-39	7.6123086373989395
1104	40-41	7.407531075580785
1104	42-43	7.467290786941721
1104	44-45	6.9772068422358515
1104	46-47	6.875856965724854
1104	48-49	5.801713851858697
1104	50-51	5.991148506917135
1104	52-53	6.224946171537852
1104	54-55	5.061572459597389
1104	56-57	6.237903945606551
1104	58-59	4.098245004103532
1104	60-61	4.364188338061453
1104	62-63	4.0509302441388115
1104	64-65	2.8855601468248864
1104	66-67	3.6040077117411684
1104	68-69	4.3946193926753025
1104	70-71	2.7458456101480593
1104	72-73	2.6515653492436613
1104	74-75	3.3503024051559898
1104	76-77	4.070303792640313
1104	78-79	4.4321677165199524
1104	80-81	3.232490673622486
1104	82-83	3.073318750894341
1104	84-85	3.249782305437922
1104	86-87	0.7712241280359144
1104	88-89	0.3839283135723157
1104	90-91	2.4861879352053897
1104	92-93	2.697179056490633
1104	94-95	2.6767098566114136
1104	96-97	3.0232969646280097
1104	98-99	2.7806022373029045
1104	100	1.08110406021693
1105	1	5.930348339269706
1105	2	-2.692895703896049
1105	3	0.5251753289389143
1105	4	8.58940961433892
1105	5	8.682441227872069
1105	6	8.195523373890637
1105	7	5.344647176233067
1105	8	6.083281987216733
1105	9	4.988558443356464
1105	10-11	5.028026100228153
1105	12-13	5.251918433833083
1105	14-15	6.058713320933297
1105	16-17	6.265872448789793
1105	18-19	6.3149364111290645
1105	20-21	7.284500928767624
1105	22-23	2.149123563612598
1105	24-25	4.285700796899455
1105	26-27	7.251562463253013
1105	28-29	6.171515225676686
1105	30-31	5.517096677416831
1105	32-33	7.416719042349847
1105	34-35	6.372202886312721
1105	36-37	6.392327768725512
1105	38-39	4.3100124266957
1105	40-41	5.5664905469127675
1105	42-43	5.854098119791207
1105	44-45	6.358232186977823
1105	46-47	5.629688599222437
1105	48-49	5.1047143484399236
1105	50-51	6.1875766236009895
1105	52-53	5.836566968105075
1105	54-55	5.453406244946972
1105	56-57	5.1646913197104745
1105	58-59	2.1717242240890187
1105	60-61	4.656971198369334
1105	62-63	-0.8677421650441204
1105	64-65	-0.5898315475357379
1105	66-67	0.8132621077583337
1105	68-69	3.0148183116561675
1105	70-71	3.126028835888789
1105	72-73	2.9272874611144957
1105	74-75	2.8394883939256133
1105	76-77	1.9367361748295977
1105	78-79	3.311937920372909
1105	80-81	1.5316275454153967
1105	82-83	0.9432281566521361
1105	84-85	1.367844333526591
1105	86-87	-0.18901929226280245
1105	88-89	1.7039623421193717
1105	90-91	1.4546287656419992
1105	92-93	1.7677989553917897
1105	94-95	1.1065639381271346
1105	96-97	2.122065061193961
1105	98-99	2.044551713346042
1105	100	2.0521285454913887
1106	1	6.22502997695716
1106	2	10.259236832744879
1106	3	6.814812821308664
1106	4	11.881519936728747
1106	5	9.999560832420645
1106	6	9.415631833555729
1106	7	6.499770298562481
1106	8	7.479253372995835
1106	9	6.920271804001366
1106	10-11	5.7242688711570615
1106	12-13	8.093236626234923
1106	14-15	7.9558383689173375
1106	16-17	7.827922657402382
1106	18-19	7.289498733745443
1106	20-21	8.254713144088537
1106	22-23	6.348250018079675
1106	24-25	5.226157243122714
1106	26-27	7.8490403905941655
1106	28-29	6.397947116656951
1106	30-31	5.452519755066021
1106	32-33	7.5530218798585835
1106	34-35	6.861681323385973
1106	36-37	6.047001021005148
1106	38-39	7.142694514331865
1106	40-41	6.662460831418798
1106	42-43	6.32847361929721
1106	44-45	4.753405071117605
1106	46-47	6.214435180556173
1106	48-49	6.106822185099102
1106	50-51	6.742620296944516
1106	52-53	5.704906075255796
1106	54-55	5.391053422226761
1106	56-57	4.998419223452913
1106	58-59	5.122208705578188
1106	60-61	5.034911361483605
1106	62-63	5.2007266563419705
1106	64-65	5.422589947426726
1106	66-67	3.0707212662598486
1106	68-69	3.2651984868529773
1106	70-71	3.086801803725322
1106	72-73	2.9300220008138176
1106	74-75	2.8178219475135613
1106	76-77	3.7177536882179325
1106	78-79	4.235764513915047
1106	80-81	3.7245266153116248
1106	82-83	2.145859727319259
1106	84-85	2.7315075925397245
1106	86-87	3.411957458289411
1106	88-89	3.4263594832144797
1106	90-91	3.2467923424510765
1106	92-93	2.9806478229684217
1106	94-95	1.4968695886019425
1106	96-97	1.5589564504971385
1106	98-99	2.117131272459236
1106	100	3.0051567317890324
1107	1	6.059532448081107
1107	2	10.385819645316687
1107	3	6.11213578212752
1107	4	11.414981177593884
1107	5	9.535738999916244
1107	6	8.000462744807486
1107	7	3.456720023906886
1107	8	6.377428039869759
1107	9	5.644012522091526
1107	10-11	4.7110356020441415
1107	12-13	6.339895232803041
1107	14-15	6.325176323527213
1107	16-17	7.129878114901075
1107	18-19	6.285691309660603
1107	20-21	5.571950843327759
1107	22-23	5.794224546265106
1107	24-25	4.6169708953276825
1107	26-27	7.241949663194848
1107	28-29	6.532747575103812
1107	30-31	5.170487035436835
1107	32-33	4.9652560648299975
1107	34-35	6.571068473039517
1107	36-37	6.621816041586474
1107	38-39	6.5704585788927545
1107	40-41	7.9090582186732306
1107	42-43	6.391545495602511
1107	44-45	4.076083117288228
1107	46-47	3.34659513063961
1107	48-49	3.919442288593153
1107	50-51	4.820123602991366
1107	52-53	3.9483914415184174
1107	54-55	5.346921841640704
1107	56-57	5.287460825137128
1107	58-59	3.0073204445502064
1107	60-61	2.771138498156077
1107	62-63	0.38094191681266665
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2207	7	5.792497587161932
2207	8	7.008245604188172
2207	9	6.315239787833967
2207	10-11	7.110420452385966
2207	12-13	8.936719672849645
2207	14-15	8.501138424226209
2207	16-17	7.62736394114188
2207	18-19	4.271181956700058
2207	20-21	7.728647684448109
2207	22-23	6.570995183853519
2207	24-25	4.639564188595152
2207	26-27	4.041109818505877
2207	28-29	5.338659544192659
2207	30-31	5.768144807678041
2207	32-33	6.943793622574425
2207	34-35	6.631603039819197
2207	36-37	6.953928036365053
2207	38-39	7.067513996648302
2207	40-41	4.154922986619113
2207	42-43	5.995366255235755
2207	44-45	4.183915303258978
2207	46-47	5.739089749524219
2207	48-49	2.4751065375519303
2207	50-51	5.609593589057052
2207	52-53	3.2211338979257
2207	54-55	3.493835764043073
2207	56-57	5.3448757766813095
2207	58-59	3.945548441729308
2207	60-61	3.828526834499172
2207	62-63	4.591244692209809
2207	64-65	3.648705119579489
2207	66-67	3.8859366466159067
2207	68-69	3.923043928797057
2207	70-71	3.932820999084118
2207	72-73	4.2611850428890925
2207	74-75	3.832854596584067
2207	76-77	4.3152058946802825
2207	78-79	4.513284777726518
2207	80-81	4.613012455429704
2207	82-83	4.113164810310625
2207	84-85	3.5412242296605214
2207	86-87	3.713749244391856
2207	88-89	3.602264862017897
2207	90-91	1.5761933729295698
2207	92-93	-0.691581760606276
2207	94-95	1.43095389940164
2207	96-97	-0.705268561210012
2207	98-99	1.0773253461166838
2207	100	2.250711850166855
2208	1	0.8455712369394135
2208	2	8.466453271812554
2208	3	6.637170905581609
2208	4	11.757208685186772
2208	5	10.016869898146613
2208	6	9.156982711128386
2208	7	7.664616533981356
2208	8	7.869697461553848
2208	9	6.597775212575826
2208	10-11	6.8916915560316525
2208	12-13	7.150862635281175
2208	14-15	8.026397483337231
2208	16-17	7.413161906866748
2208	18-19	6.649488021082856
2208	20-21	6.644271544330923
2208	22-23	6.081518786880942
2208	24-25	6.374125624536788
2208	26-27	8.343067269638464
2208	28-29	7.4019378539911145
2208	30-31	6.2577446127715675
2208	32-33	8.62850827802336
2208	34-35	6.802216848306443
2208	36-37	7.06921356455916
2208	38-39	7.492369347149406
2208	40-41	7.857544368545213
2208	42-43	7.181080908236115
2208	44-45	6.0554475105748296
2208	46-47	4.7526956172865376
2208	48-49	4.821081388250139
2208	50-51	6.106579761960642
2208	52-53	5.748009933978501
2208	54-55	6.5893231649064425
2208	56-57	6.627211397798792
2208	58-59	5.9977519133172805
2208	60-61	5.318755800637845
2208	62-63	3.4937672129675903
2208	64-65	3.492589622800587
2208	66-67	3.0484281072146793
2208	68-69	5.7021274153703025
2208	70-71	5.411984676450238
2208	72-73	4.811837526599952
2208	74-75	2.28614492888002
2208	76-77	3.5964620008112647
2208	78-79	4.400481980965491
2208	80-81	3.720002950066103
2208	82-83	1.4416466210799435
2208	84-85	2.03555351631751
2208	86-87	1.1219424172783086
2208	88-89	0.7935689969245239
2208	90-91	2.203176656516046
2208	92-93	1.0471426181293282
2208	94-95	-0.9199947393137435
2208	96-97	1.483099978534014
2208	98-99	0.3743920770992233
2208	100	1.8402336681839522
2209	1	5.461112468268741
2209	2	9.770649448365248
2209	3	5.696180845230916
2209	4	-3.461199857936638
2209	5	0.5973569731966535
2209	6	4.590646636277228
2209	7	2.480741688537641
2209	8	5.568127875189955
2209	9	5.3508476364502044
2209	10-11	5.210599210285359
2209	12-13	7.473817778835304
2209	14-15	6.92770726613433
2209	16-17	6.519263603534412
2209	18-19	5.551168799785014
2209	20-21	6.171061265068321
2209	22-23	5.708526467712552
2209	24-25	5.524886880765408
2209	26-27	7.221290418201651
2209	28-29	6.463119700979021
2209	30-31	4.132868896399756
2209	32-33	6.400209440213644
2209	34-35	5.728311164588362
2209	36-37	6.397478541390154
2209	38-39	7.027570214063445
2209	40-41	7.424160742966443
2209	42-43	7.034934962704394
2209	44-45	6.813979123987366
2209	46-47	7.0945277775227105
2209	48-49	5.458733838678093
2209	50-51	4.866884431704346
2209	52-53	5.105144823832248
2209	54-55	5.6580973214533365
2209	56-57	5.292181295434332
2209	58-59	3.3243084540054966
2209	60-61	4.291037845105052
2209	62-63	4.490797162676358
2209	64-65	5.316607061432698
2209	66-67	4.706031181736808
2209	68-69	3.8849382780087645
2209	70-71	3.7977671858787048
2209	72-73	0.9717659852092808
2209	74-75	2.66418896548576
2209	76-77	3.6187226217854267
2209	78-79	3.783734252707223
2209	80-81	3.91903278056963
2209	82-83	3.245295806743929
2209	84-85	3.213364342464569
2209	86-87	1.7479904807100937
2209	88-89	2.229516462687986
2209	90-91	2.564842896436055
2209	92-93	2.5618502794380404
2209	94-95	0.9045168689048495
2209	96-97	0.34143719921515014
2209	98-99	0.5759799699823667
2209	100	0.4467198929843583
2210	1	1.8545434721657443
2210	2	-17.14626449822229
2210	3	-7.601698284399497
2210	4	-5.699135465906439
2210	5	0.11768647813711297
2210	6	3.5433221016928798
2210	7	-5.605907756572506
2210	8	-5.461471046231196
2210	9	-1.8021847357434524
2210	10-11	-0.6768759552043413
2210	12-13	3.5451005730060814
2210	14-15	-3.1298015902041527
2210	16-17	-0.11505296513036711
2210	18-19	-0.2534535596761778
2210	20-21	2.770358209668391
2210	22-23	2.8006373600573795
2210	24-25	2.535273803458889
2210	26-27	4.400920956281492
2210	28-29	3.721165822712159
2210	30-31	2.776382192490658
2210	32-33	4.696413355540052
2210	34-35	2.3384993891991215
2210	36-37	4.195335494698835
2210	38-39	3.372308483526254
2210	40-41	4.26747070770741
2210	42-43	2.7006187724451074
2210	44-45	3.7043408413551617
2210	46-47	3.1429029813501685
2210	48-49	3.2319048938437653
2210	50-51	2.017964781578062
2210	52-53	2.373699486023291
2210	54-55	2.815534047545359
2210	56-57	2.9775686570341726
2210	58-59	2.009567883749302
2210	60-61	2.9524129143345554
2210	62-63	2.1717277436172324
2210	64-65	2.890523513812589
2210	66-67	2.7912245577223125
2210	68-69	3.43855395786505
2210	70-71	2.478176161252115
2210	72-73	1.055267118643723
2210	74-75	0.38726848392341395
2210	76-77	-0.5402308063109089
2210	78-79	0.339982072750594
2210	80-81	1.1843883763486112
2210	82-83	0.9727845411504887
2210	84-85	-0.5382487759385519
2210	86-87	-0.4612817474113946
2210	88-89	-1.5181551225313719
2210	90-91	-1.3190411789541088
2210	92-93	-0.7955065150943312
2210	94-95	-0.119202357213684
2210	96-97	0.27002187512162834
2210	98-99	-0.07489524891530763
2210	100	-0.07174152085383412
2211	1	1.3176082830270914
2211	2	-17.14626449822229
2211	3	-7.9577222939735215
2211	4	-19.218923718994414
2211	5	-21.311353098369064
2211	6	-12.030446556449073
2211	7	-9.480480146079818
2211	8	-20.481430442692304
2211	9	-14.388560481385678
2211	10-11	-9.002304755809678
2211	12-13	-22.41368440304635
2211	14-15	-17.86502006772064
2211	16-17	-16.6460674300872
2211	18-19	-18.951183661272985
2211	20-21	-7.416783425206599
2211	22-23	-2.913294486675774
2211	24-25	-1.8268803642509255
2211	26-27	-17.401374684775703
2211	28-29	-3.9574243353494616
2211	30-31	-3.303240741876955
2211	32-33	0.14006160791568334
2211	34-35	-2.9287025033214604
2211	36-37	-3.8707498220621517
2211	38-39	-1.0586223372090622
2211	40-41	-2.575461760902723
2211	42-43	-0.39038780499703307
2211	44-45	0.5864861849833716
2211	46-47	0.6337365267621529
2211	48-49	0.4916524985480173
2211	50-51	-2.76073834232103
2211	52-53	-0.40480213800615417
2211	54-55	-0.45371725345043146
2211	56-57	-4.147100946566038
2211	58-59	-1.004375363918058
2211	60-61	-0.5068064528342546
2211	62-63	-0.23244598143794803
2211	64-65	-2.435657601842127
2211	66-67	-1.5947661433583882
2211	68-69	0.3834357878719423
2211	70-71	-0.2115992446560675
2211	72-73	-1.2634337509405427
2211	74-75	-1.9772879434136108
2211	76-77	-1.7392111298680426
2211	78-79	-1.1112453861493385
2211	80-81	-1.0585424623246311
2211	82-83	-1.6910432612780504
2211	84-85	-1.7786301001044542
2211	86-87	-2.2820511707612816
2211	88-89	-2.833612935150896
2211	90-91	-2.4936401907639087
2211	92-93	-2.364157731971577
2211	94-95	-2.5106000073966293
2211	96-97	-3.1872710187588744
2211	98-99	-3.131845071265122
2211	100	-2.535645964812076
2212	1	-21.375857334489158
2212	2	-17.14626449822229
2212	3	-20.096278571770306
2212	4	-19.218923718994414
2212	5	-21.311353098369064
2212	6	-22.06671066866255
2212	7	-22.47911168901516
2212	8	-22.97646978583291
2212	9	-24.252399003452048
2212	10-11	-11.448569644452697
2212	12-13	-22.41368440304635
2212	14-15	-23.801215757080886
2212	16-17	-24.164712657593185
2212	18-19	-18.77070188674327
2212	20-21	-23.94466573789904
2212	22-23	-18.922064379649814
2212	24-25	-25.50871409671757
2212	26-27	-17.43336661476117
2212	28-29	-18.34311243389778
2212	30-31	-6.3532933935051545
2212	32-33	-17.604380248430296
2212	34-35	-14.107229737800754
2212	36-37	-17.985065848295765
2212	38-39	-24.022700339261746
2212	40-41	-10.419325055924112
2212	42-43	-14.302905426308083
2212	44-45	-17.39096994068175
2212	46-47	-17.255240478791748
2212	48-49	-17.862306928155817
2212	50-51	-9.137423376399006
2212	52-53	-2.1709684928878374
2212	54-55	-23.77496254937927
2212	56-57	-23.746656198020023
2212	58-59	-12.72286797449254
2212	60-61	-6.273436603049106
2212	62-63	-3.1363591863207176
2212	64-65	-17.523253533351337
2212	66-67	-17.854911651106914
2212	68-69	-14.731724241535323
2212	70-71	-9.743829320139579
2212	72-73	-17.659857928956427
2212	74-75	-6.347745648627576
2212	76-77	-11.093405669142031
2212	78-79	-4.582122363315033
2212	80-81	-2.184773163804458
2212	82-83	-2.9122682972485734
2212	84-85	-5.484194673565607
2212	86-87	-4.78520723552521
2212	88-89	-3.366339076320344
2212	90-91	-4.043864142159421
2212	92-93	-5.3096833141117585
2212	94-95	-3.4324431540714464
2212	96-97	-5.438927846607459
2212	98-99	-3.5256610526114045
2212	100	-3.4936161353596766
2213	1	-21.375857334489158
2213	2	-17.14626449822229
2213	3	-20.096278571770306
2213	4	-19.218923718994414
2213	5	-21.311353098369064
2213	6	-22.06671066866255
2213	7	-10.848038814521232
2213	8	-10.64246168866692
2213	9	-4.6435933354358525
2213	10-11	-17.833022080780832
2213	12-13	-16.431903026528133
2213	14-15	-23.801215757080886
2213	16-17	-18.112334115083065
2213	18-19	-18.83152789817119
2213	20-21	-17.96971634518649
2213	22-23	-20.102321794873635
2213	24-25	-7.854867942871415
2213	26-27	-23.50606165022216
2213	28-29	-24.417225909075086
2213	30-31	-19.798082278565595
2213	32-33	-23.696578829990578
2213	34-35	-18.26711107986295
2213	36-37	-24.124427550423423
2213	38-39	-11.859997910111948
2213	40-41	-17.493690131870935
2213	42-43	-23.567089823471203
2213	44-45	-17.493004999687695
2213	46-47	-17.243271316934567
2213	48-49	-6.62764955025936
2213	50-51	-23.646643234555036
2213	52-53	-18.05372217537556
2213	54-55	-18.000671051403565
2213	56-57	-17.881524619072657
2213	58-59	-18.29204957210359
2213	60-61	-19.51038817797661
2213	62-63	-17.8555856485483
2213	64-65	-17.928633698165992
2213	66-67	-9.11071964490483
2213	68-69	-22.963639135152345
2213	70-71	-17.65235287944701
2213	72-73	-9.741380885283412
2213	74-75	-4.5506248802582085
2213	76-77	-3.456489268073895
2213	78-79	-4.815582394325453
2213	80-81	-3.730401555569472
2213	82-83	-3.55291635766147
2213	84-85	-3.164353027716121
2213	86-87	-3.588703804279337
2213	88-89	-3.212413242915229
2213	90-91	-2.972274929766737
2213	92-93	-4.159433507926899
2213	94-95	-4.011477021473176
2213	96-97	-3.691170359600253
2213	98-99	-4.0347470347517
2213	100	-3.538572634627485
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	664.0
3	625.0
4	3669.0
5	12793.0
6	16950.0
7	19080.0
8	18092.0
9	18014.0
10	21856.0
11	27520.0
12	34493.0
13	43627.0
14	36070.0
15	41140.0
16	46655.0
17	49178.0
18	49728.0
19	50933.0
20	51214.0
21	51354.0
22	54378.0
23	60040.0
24	66283.0
25	73349.0
26	79544.0
27	86301.0
28	93396.0
29	97697.0
30	104087.0
31	115530.0
32	132555.0
33	158914.0
34	189507.0
35	218451.0
36	228260.0
37	183093.0
38	51078.0
39	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40181964892919	20.86129327991657	14.133178193343213	23.603708877811027
2	14.676087533169877	21.499273313719698	40.26134482852524	23.563294324585193
3	17.74608423015762	27.27768251785424	30.09639794631709	24.87983530567105
4	12.124855341070138	18.384968968628694	38.898817483547	30.591358206754165
5	12.796447394704897	38.74956371706475	35.38600338489704	13.067985503333313
6	30.13407768625117	38.150914698156605	18.810830894831028	12.904176720761196
7	26.438651102248024	32.4871521833575	23.6445688945666	17.42962781982787
8	24.057376410169105	36.63816444032969	21.75643723154059	17.548021917960615
9	24.889987793555257	17.888163319633545	21.97151937773103	35.25032950908017
10-11	22.906147530923914	28.096872409428087	28.898050765772798	20.0989292938752
12-13	24.095647549544125	25.72063500199693	28.70105655686762	21.48266089159133
14-15	21.79329927215073	27.12795599294101	26.33064547918922	24.74809925571904
16-17	20.98207838847822	30.389846052475566	27.428798983421633	21.19927657562458
18-19	21.189121057044076	29.582504978365314	28.777154532741918	20.451219431848692
20-21	22.491113010943327	28.771487862132965	27.881238143829236	20.856160983094476
22-23	21.604119345508817	28.78158425620228	27.94355812083147	21.670738277457435
24-25	21.762376530857118	28.8954460062564	27.517342428189455	21.824835034697028
26-27	21.796760442437133	28.4355301152219	27.787210104873296	21.98049933746767
28-29	21.692289519231263	28.29237477466373	28.330667817101418	21.684667889003588
30-31	22.255055950269114	28.83598687500566	27.66465860827164	21.244298566453583
32-33	21.266963751269504	28.64164882375812	28.22778320939831	21.863604215574068
34-35	21.21116721214821	28.4269859154697	28.15756193489812	22.204284937483976
36-37	22.11220802071822	28.659670360414857	27.627204880468305	21.600916738398613
38-39	22.032699883485055	28.27006507037441	27.821988896811135	21.8752461493294
40-41	21.732482968958134	28.509121884120788	27.62405942129358	22.1343357256275
42-43	21.85608225162931	28.37425181753194	27.96569369298284	21.803972237855913
44-45	22.255097126937684	28.05225152955888	27.892490987682656	21.80016035582078
46-47	21.84507373996229	28.43162633917088	28.27061785471133	21.452682066155496
48-49	21.93185927042755	28.215260752784182	28.170033184273645	21.682846792514617
50-51	21.866742970373284	28.29379785688561	28.02312035294862	21.816338819792485
52-53	21.40937583691255	28.389624197319062	27.762400401113936	22.438599564654453
54-55	21.7906726479528	28.585550950918925	27.52965750253866	22.094118898589617
56-57	22.065722388530652	28.155263905854728	27.55109935606014	22.22791434955448
58-59	21.858831869465437	27.712881636968774	28.12456694742491	22.303719546140876
60-61	22.202124891169202	28.248719732187894	27.72309911553895	21.826056261103954
62-63	21.557659661855684	28.36404464435775	27.978105449073986	22.10019024471258
64-65	21.415906377362106	28.109118937626892	28.090418591529424	22.384556093481578
66-67	21.849255117451406	28.079564909074183	27.68832725578801	22.3828527176864
68-69	22.463969674818667	27.899686202379282	27.550120481487728	22.086223641314326
70-71	22.07481254732305	28.141795478982434	27.552062276142696	22.23132969755182
72-73	22.115607920549714	28.127437119377856	27.730358288912704	22.02659667115973
74-75	22.346442701064426	27.81479679616593	27.520460219086285	22.318300283683357
76-77	22.072180166673636	28.08399575870585	27.867612372603865	21.976211702016645
78-79	22.2048417951315	27.945200207203662	27.947929671714146	21.90202832595069
80-81	22.05142141991337	27.858865325743498	27.88614067581819	22.203572578524945
82-83	21.579106333144317	28.113073351143537	27.88910128627075	22.418719029441394
84-85	21.7951217909363	27.958961067307975	27.510331219008542	22.735585922747184
86-87	22.161607156545156	27.830077462095154	27.600196464226105	22.408118917133585
88-89	21.958967156485315	27.53299168808517	28.01716748886851	22.490873666561008
90-91	22.178431816185764	27.70668622500268	27.72948618847621	22.385395770335347
92-93	21.656975982844084	27.904222378156085	27.94775624989451	22.49104538910532
94-95	21.44217498800335	27.700995124181105	28.05866919900093	22.798160688814615
96-97	21.952097342124834	27.702791073059256	27.84980625672524	22.495305328090666
98-99	22.005621816079202	27.447409302732872	27.80944695767794	22.73752192350998
100	21.80845960807283	27.489278962919055	27.832787166682653	22.869474262325454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	351.0
1	408.0
2	376.5
3	300.5
4	339.5
5	440.0
6	618.5
7	858.0
8	1279.5
9	1824.5
10	2423.5
11	3136.0
12	3919.5
13	4642.5
14	5340.0
15	5990.5
16	6621.5
17	7304.0
18	8081.5
19	8798.5
20	9594.5
21	10497.0
22	11471.0
23	12856.5
24	14615.0
25	17155.5
26	20631.0
27	25281.0
28	31369.0
29	38422.0
30	46264.5
31	54162.5
32	61750.5
33	69251.5
34	76125.5
35	82195.5
36	87615.0
37	92010.5
38	95127.0
39	96840.0
40	97299.5
41	96895.0
42	95146.5
43	92326.0
44	89243.0
45	85847.0
46	83065.0
47	81100.5
48	79453.5
49	80504.5
50	84190.5
51	80544.0
52	69162.5
53	59614.0
54	53418.5
55	48722.5
56	44012.5
57	39684.5
58	35540.0
59	31365.0
60	27281.5
61	23054.0
62	19205.0
63	15779.0
64	12680.0
65	10174.0
66	8232.0
67	6518.5
68	4999.0
69	3843.5
70	2930.0
71	2205.0
72	1647.0
73	1236.5
74	940.5
75	665.5
76	463.5
77	327.0
78	227.5
79	163.0
80	104.5
81	68.5
82	51.5
83	38.5
84	31.5
85	20.5
86	14.5
87	10.5
88	6.5
89	5.5
90	4.0
91	1.5
92	1.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.0836331634178045
2	23.324415442502804
3	5.580802934977246
4	22.65079826014128
5	11.925742415378316
6	8.83632931416019
7	7.258755844028293
8	8.242095409187348
9	4.7140829336780365
10-11	6.2691715641599
12-13	9.476963576106446
14-15	6.813505751904252
16-17	7.221654891931635
18-19	6.0969682393423215
20-21	7.1232474681838545
22-23	5.333489032308955
24-25	4.69225543241846
26-27	9.129772905052883
28-29	5.889925979830538
30-31	3.9291242280623946
32-33	8.206212466461242
34-35	6.3478781696760525
36-37	6.29548443326024
38-39	5.502096329461095
40-41	7.487625604799653
42-43	5.9111541431458745
44-45	5.81373272698378
46-47	5.523092490991564
48-49	3.6479960652497283
50-51	6.217319165242111
52-53	3.986641337227354
54-55	4.404959888824745
56-57	4.293328363339542
58-59	3.4559372543439437
60-61	3.715237915897033
62-63	2.7963755134489503
64-65	4.0580785534843375
66-67	2.25967805112314
68-69	4.705788869869039
70-71	2.2561786908680617
72-73	1.4334230278011606
74-75	0.7303416187615203
76-77	1.064656190754961
78-79	1.5428118693659816
80-81	1.8275747268082316
82-83	0.7826966882131879
84-85	1.1498201792832459
86-87	0.37261419997857853
88-89	0.6404215936434604
90-91	0.27977481906760804
92-93	0.3434979759777619
94-95	0.5256193770984078
96-97	0.5151792967793899
98-99	0.4269026176374717
100	0.44760877693685724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2586187.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.59165205570231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.37580601220199	90.19970902881872
2	2.8380024051675954	5.312266672753838
3	0.4552412750965137	1.2782034906068152
4	0.1448237194241659	0.5421716463103676
5	0.06032995299099333	0.2823189984434962
6	0.03540157172348997	0.19879749497819177
7	0.020117687910528727	0.13179933529611895
8	0.014777895492403725	0.11064701224324648
9	0.010929802780600371	0.09206444689914578
>10	0.04065865716334267	0.6224947382893334
>50	0.0019473889902408288	0.11958985693827531
>100	0.0014212545203110976	0.34679043956969696
>500	2.0860636075264506E-4	0.13506574477654684
>1k	3.337701771395969E-4	0.6280810940762114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4581	0.17713336274600408	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3729	0.14418910929488085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.8666964144510816E-5	0.0
5	0.0	0.0	0.0	3.8666964144510816E-5	0.0
6	0.0	0.0	0.0	3.8666964144510816E-5	0.0
7	0.0	0.0	0.0	1.1600089243353246E-4	0.0
8	0.0	0.0	0.0	1.1600089243353246E-4	0.0
9	0.0	0.0	0.0	1.5466785657804327E-4	0.0
10-11	0.0	0.0	0.0	1.7400133865029868E-4	0.0
12-13	0.0	0.0	0.0	2.320017848670649E-4	0.0
14-15	0.0	0.0	0.0	4.446700876618744E-4	0.0
16-17	0.0	0.0	0.0	5.413374980231515E-4	0.0
18-19	1.9333482072255408E-5	0.0	0.0	5.606709800954068E-4	0.0
20-21	3.8666964144510816E-5	0.0	0.0	8.313397291069826E-4	0.0
22-23	3.8666964144510816E-5	0.0	0.0	8.50673211179238E-4	3.8666964144510816E-5
24-25	3.8666964144510816E-5	1.9333482072255408E-5	0.0	8.893401753237488E-4	3.8666964144510816E-5
26-27	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.0010633415139740475	3.8666964144510816E-5
28-29	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.0013533437450578786	3.8666964144510816E-5
30-31	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.0030546901674163547	3.8666964144510816E-5
32-33	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.007482057561962843	3.8666964144510816E-5
34-35	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.013282102183639465	3.8666964144510816E-5
36-37	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.023490180717790323	3.8666964144510816E-5
38-39	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.04195365609679424	3.8666964144510816E-5
40-41	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.06751251939631589	3.8666964144510816E-5
42-43	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.09019069386707149	3.8666964144510816E-5
44-45	3.8666964144510816E-5	3.8666964144510816E-5	0.0	0.1183209102822031	3.8666964144510816E-5
46-47	3.8666964144510816E-5	9.666741036127704E-5	0.0	0.1524058391755894	3.8666964144510816E-5
48-49	3.8666964144510816E-5	1.1600089243353246E-4	0.0	0.1875154426188052	3.8666964144510816E-5
50-51	3.8666964144510816E-5	1.1600089243353246E-4	0.0	0.22682041167170047	3.8666964144510816E-5
52-53	3.8666964144510816E-5	1.1600089243353246E-4	0.0	0.2697214083900352	3.8666964144510816E-5
54-55	3.8666964144510816E-5	1.3533437450578785E-4	0.0	0.3177264443754454	5.800044621676623E-5
56-57	3.8666964144510816E-5	1.5466785657804327E-4	0.0	0.3751662196121162	1.1600089243353246E-4
58-59	3.8666964144510816E-5	1.5466785657804327E-4	0.0	0.43856070732704167	1.1600089243353246E-4
60-61	7.733392828902163E-5	1.933348207225541E-4	0.0	0.5060152262771408	1.1600089243353246E-4
62-63	7.733392828902163E-5	1.933348207225541E-4	0.0	0.5738177479045405	1.1600089243353246E-4
64-65	7.733392828902163E-5	1.933348207225541E-4	0.0	0.6554823761777474	1.1600089243353246E-4
66-67	7.733392828902163E-5	1.933348207225541E-4	0.0	0.751589115558929	1.1600089243353246E-4
68-69	7.733392828902163E-5	1.933348207225541E-4	0.0	0.8595859464145477	1.1600089243353246E-4
70-71	7.733392828902163E-5	1.933348207225541E-4	0.0	0.9898742820994769	1.1600089243353246E-4
72-73	7.733392828902163E-5	1.933348207225541E-4	0.0	1.1267553351710453	1.1600089243353246E-4
74-75	7.733392828902163E-5	2.1266830279480952E-4	0.0	1.2675610851032815	1.1600089243353246E-4
76-77	7.733392828902163E-5	2.7066874901157575E-4	0.0	1.4274489818408336	1.1600089243353246E-4
78-79	7.733392828902163E-5	2.7066874901157575E-4	0.0	1.6036350039652971	1.1600089243353246E-4
80-81	7.733392828902163E-5	2.7066874901157575E-4	0.0	1.8057085585845107	1.1600089243353246E-4
82-83	7.733392828902163E-5	2.7066874901157575E-4	0.0	2.0324903032920667	1.1600089243353246E-4
84-85	7.733392828902163E-5	2.7066874901157575E-4	0.0	2.2598713859438626	1.1600089243353246E-4
86-87	7.733392828902163E-5	3.0933571315608653E-4	0.0	2.513932673855371	1.1600089243353246E-4
88	7.733392828902163E-5	3.0933571315608653E-4	0.0	2.7285729918215504	1.1600089243353246E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGA	1020	0.0	18.665855	1
GTATATA	1510	0.0	18.302986	1
GTATAGG	675	0.0	18.197535	1
GTACTAA	590	0.0	17.696333	1
CATGGGG	4495	0.0	17.455107	4
GTATCGT	200	0.0018396148	16.693766	4
GTCTAGG	635	1.8189894E-12	16.442263	1
GTACTAG	600	1.2732926E-11	16.37778	1
GTACATG	16870	0.0	15.254054	1
GTGTAGG	740	1.8189894E-12	14.939193	1
TATACTG	1190	0.0	14.673164	5
TACATGG	16895	0.0	14.646983	2
CCTAGTA	510	1.0885378E-7	14.448156	2
CCTAGAC	720	5.456968E-12	13.912529	3
TATCGTT	220	0.007242216	13.586271	7
ACATGGG	17245	0.0	13.521252	3
TACATCG	410	4.3358596E-5	13.479072	2
GTATTAT	1285	0.0	13.382624	1
CTAGTAC	505	3.2062053E-7	13.223789	3
CCTATAC	725	8.731149E-11	13.048991	3
>>END_MODULE