FastQCFastQC Report
Wed 25 May 2016
SRR1295143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586187
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT121470.46968761346337295No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA97160.37568822362806714No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39720.15358518158199697No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35030.1354503753982214No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32860.12705964417886256No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30860.11932625134996037No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30350.11735423617859034No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG26610.10289279158854328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.10014743713428302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT49100.019.6046512-13
TCTATAC8550.019.2509783
ACGCGGA1300.00808556418.0598187
GTCTCGC4403.6379788E-1217.9653631
AGTACTT56650.016.66030512-13
CATGGGG54150.016.5880074
GTACTTT59350.016.3767614-15
GTCTTAC8550.016.1793331
CTATACT12350.015.9934314
TAGACTG10950.015.8908425
GTATCAA185950.015.4364921
CTATACA11200.014.6963444
CCTATAC8200.014.337623
TATACTA10850.014.3035395
CAACGCA200900.014.18573955
AACGCAG201100.014.1760686
ACCGTTC2651.5791153E-414.1752568
TCAACGC204800.014.03041654
ATCAACG205550.013.9789523
GTACATG218350.013.9152361
Produced by FastQC (version 0.11.5)