FastQCFastQC Report
Wed 25 May 2016
SRR1295142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505549
Sequences flagged as poor quality0
Sequence length100
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68731.3595121343331704No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54131.0707171807282776No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24940.4933250782812349No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24870.4919404449420333No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21740.43002755420345007No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC18910.3740488063471592No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG17810.3522902824454207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16820.3327076109338561No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16100.3184656680163545No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT15970.31589420610069446No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14590.2885971488421498No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13020.2575418010914867No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10080.19938720084502196No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC9670.19127720557255576No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC9220.1823759912491173No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA9060.17921111504522805No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT8830.17466160550213727No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA7540.1491447911082803No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC7500.148353572057308No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC7250.14340845298873106No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7230.14301284346324492No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7170.14182601488678645No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG7100.1404413815475849No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG6790.1343094339025495No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA6630.13114455769866026No Hit
CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5950.11769383383213101No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5700.11274871476355407No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC5400.10681457188126177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA3250.043.4684521
TTGTTCG703.1002328E-642.704235
GTACAAA2450.037.6058041
TGTTCGC851.1294393E-535.374026
GGTATGG1051.19091E-535.098751
TACATCG1301.3536992E-633.0643352
TCGCTAT952.400535E-531.6838179
TACATAA2357.2759576E-1231.355842
TAGGTGT803.2798416E-431.3207456
CATAGTC650.004517853330.8064337
GGATATA800.004585499430.7114091
GTATAAG1003.6788307E-430.7114071
ATAGGTG1003.5718986E-529.8929585
GTACATC1902.7181159E-829.0950161
ACGACTG1054.753573E-528.666319
AGCGTTC350.00759779627.70065138-39
TATAGGT1058.6736894E-426.5242334
CACGGGA1100.001137881225.3185834
TTCGCTA1201.1821794E-425.0742058
GTTCGCT1201.1963628E-425.0302287
Produced by FastQC (version 0.11.5)