##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295142_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 505549 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.470245218564372 33.0 27.0 33.0 15.0 33.0 2 22.717153035610792 27.0 6.0 33.0 2.0 33.0 3 24.983681107073696 27.0 15.0 33.0 15.0 33.0 4 24.961499280979687 33.0 6.0 37.0 2.0 37.0 5 27.026559245493512 33.0 15.0 37.0 2.0 37.0 6 27.831248800808627 33.0 15.0 37.0 6.0 37.0 7 27.564145117486138 33.0 15.0 37.0 6.0 37.0 8 28.28377071263122 33.0 22.0 37.0 6.0 37.0 9 29.072920725785234 33.0 22.0 37.0 15.0 37.0 10-11 28.720411869076983 33.0 24.5 37.0 10.5 37.0 12-13 27.538436432472423 33.0 15.0 37.0 4.0 37.0 14-15 29.324324645088804 33.0 22.0 37.0 6.0 40.0 16-17 29.733663799156957 35.0 27.0 37.0 6.0 40.0 18-19 29.87279472415137 35.0 27.0 40.0 6.0 40.0 20-21 29.497817224443132 33.0 24.5 38.5 6.0 40.0 22-23 30.293663917839815 35.0 27.0 40.0 15.0 40.0 24-25 30.491685276798094 35.0 27.0 40.0 15.0 40.0 26-27 29.150563051257148 33.0 24.5 37.0 6.0 40.0 28-29 29.800281476177382 33.0 27.0 37.0 10.5 40.0 30-31 30.4468617285367 33.0 27.0 38.5 15.0 40.0 32-33 29.14512539832934 33.0 27.0 37.0 6.0 40.0 34-35 28.846198884776747 33.0 22.0 37.0 6.0 40.0 36-37 29.1643688346728 33.0 24.5 37.0 6.0 40.0 38-39 29.211680766849504 33.0 24.5 37.0 6.0 40.0 40-41 28.249004547531495 33.0 22.0 37.0 6.0 40.0 42-43 28.726777226342058 33.0 22.0 37.0 6.0 40.0 44-45 28.61530830839345 33.0 22.0 37.0 6.0 40.0 46-47 28.148623575558453 33.0 22.0 37.0 6.0 40.0 48-49 28.003222239585085 33.0 22.0 37.0 6.0 40.0 50-51 28.071014876896207 33.0 22.0 37.0 6.0 40.0 52-53 28.29363622517303 33.0 22.0 37.0 6.0 40.0 54-55 28.218113377733907 33.0 22.0 37.0 6.0 40.0 56-57 28.12303555145001 33.0 22.0 37.0 6.0 40.0 58-59 27.920338087900483 33.0 22.0 37.0 6.0 40.0 60-61 27.785170181327626 33.0 22.0 37.0 6.0 40.0 62-63 27.55304827029625 33.0 22.0 37.0 6.0 40.0 64-65 27.240999388783283 33.0 22.0 37.0 6.0 37.0 66-67 27.444670051765506 33.0 22.0 37.0 6.0 37.0 68-69 26.9577647270591 33.0 22.0 37.0 6.0 37.0 70-71 26.878541941532866 27.0 22.0 37.0 6.0 37.0 72-73 26.770050974287358 27.0 22.0 35.0 6.0 37.0 74-75 26.84819770190427 27.0 22.0 33.0 6.0 37.0 76-77 26.47675299525862 27.0 22.0 33.0 6.0 37.0 78-79 26.07115927437301 27.0 22.0 33.0 6.0 37.0 80-81 26.02314909138382 27.0 22.0 33.0 6.0 37.0 82-83 26.13016740217071 27.0 22.0 33.0 6.0 37.0 84-85 25.976521563686212 27.0 22.0 33.0 6.0 37.0 86-87 25.579231686740556 27.0 22.0 33.0 6.0 37.0 88-89 25.449259122261147 27.0 22.0 33.0 6.0 37.0 90-91 25.584394391048146 27.0 22.0 33.0 6.0 37.0 92-93 25.428224563791048 27.0 22.0 33.0 6.0 37.0 94-95 25.357343205109693 27.0 22.0 33.0 6.0 37.0 96-97 25.510306617162726 27.0 22.0 33.0 6.0 37.0 98-99 25.51414304053613 27.0 22.0 33.0 6.0 37.0 100 24.990082069196063 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.386626089186471 1101 2 11.506184880700431 1101 3 8.363544374120153 1101 4 12.676682898643271 1101 5 10.631135931464126 1101 6 9.70219496374363 1101 7 9.514387033353646 1101 8 8.909069205402549 1101 9 7.142385351153791 1101 10-11 7.666063429415569 1101 12-13 8.866852151680366 1101 14-15 7.591522446370082 1101 16-17 7.899454284260056 1101 18-19 7.99028307849165 1101 20-21 6.9267695221472785 1101 22-23 6.849729708687981 1101 24-25 6.934268899509849 1101 26-27 8.414468477921492 1101 28-29 8.06383849927548 1101 30-31 5.254665941567378 1101 32-33 6.367037807188034 1101 34-35 8.491353904464841 1101 36-37 7.9273417026163 1101 38-39 7.846398984303171 1101 40-41 8.743310820663517 1101 42-43 8.50129907664149 1101 44-45 8.401841743970568 1101 46-47 7.182678246422171 1101 48-49 5.074553289148174 1101 50-51 6.516445350268555 1101 52-53 5.589037522306015 1101 54-55 3.511353125730377 1101 56-57 5.908912860884072 1101 58-59 6.095073587467336 1101 60-61 6.433646312045688 1101 62-63 5.8427099133911256 1101 64-65 6.409069634285739 1101 66-67 5.408641964692762 1101 68-69 6.425279412168724 1101 70-71 5.939028932957111 1101 72-73 5.187981775913883 1101 74-75 4.684255509139888 1101 76-77 4.958192243357942 1101 78-79 4.678223041726216 1101 80-81 4.631129582246633 1101 82-83 4.3949640083713675 1101 84-85 4.091220751214028 1101 86-87 4.114357853582199 1101 88-89 3.993484355218449 1101 90-91 3.704854385826568 1101 92-93 3.5323511530398264 1101 94-95 3.4815631824287543 1101 96-97 3.771832176043862 1101 98-99 3.426745220492112 1101 100 3.489309936524723 1102 1 6.732206322150624 1102 2 10.270061705514369 1102 3 8.013482190189109 1102 4 12.350368306801336 1102 5 10.473286828521278 1102 6 9.203163620688414 1102 7 6.653908158235456 1102 8 7.641150228906898 1102 9 6.183824565870779 1102 10-11 7.237598097447535 1102 12-13 8.790374155376089 1102 14-15 5.847665708731345 1102 16-17 6.843999949235791 1102 18-19 7.075374628146598 1102 20-21 6.4095090675964705 1102 22-23 6.186342508769577 1102 24-25 6.660286891655449 1102 26-27 8.23382513905634 1102 28-29 7.332611180717198 1102 30-31 3.6601620594853763 1102 32-33 3.9809462143770453 1102 34-35 4.577424703910875 1102 36-37 4.942615409994804 1102 38-39 4.28542891497888 1102 40-41 6.11298154007471 1102 42-43 6.812644015026439 1102 44-45 6.394667640733477 1102 46-47 5.272804536689698 1102 48-49 3.3083840939298668 1102 50-51 4.753327679439295 1102 52-53 5.541918580335192 1102 54-55 5.269782873581402 1102 56-57 6.284247614443466 1102 58-59 5.830259099592222 1102 60-61 5.536810434467608 1102 62-63 3.9720834838472854 1102 64-65 5.772787958732852 1102 66-67 3.264828775422135 1102 68-69 5.796095728290641 1102 70-71 4.782764833682524 1102 72-73 4.375548128326109 1102 74-75 3.9253549681200717 1102 76-77 3.100557743634532 1102 78-79 3.7389745845546614 1102 80-81 3.8542380726091032 1102 82-83 3.2467967669447084 1102 84-85 2.4181928841817104 1102 86-87 2.7198068921833176 1102 88-89 2.8730644861774124 1102 90-91 2.8323420373140777 1102 92-93 2.6178205142127275 1102 94-95 2.486197356520222 1102 96-97 2.904626058871287 1102 98-99 2.769919308036105 1102 100 2.9148128024781386 1103 1 6.903813444328968 1103 2 11.13709867008317 1103 3 8.137034174983203 1103 4 12.334842948316492 1103 5 10.584575856549183 1103 6 9.2570735113161 1103 7 8.231958623636832 1103 8 8.250419123483805 1103 9 7.451000491981574 1103 10-11 7.663240095778402 1103 12-13 8.880417241440409 1103 14-15 8.642597206281547 1103 16-17 8.138653124387229 1103 18-19 7.508395963535072 1103 20-21 8.326816967240685 1103 22-23 6.9258798626702855 1103 24-25 5.277679446032135 1103 26-27 6.178991234037351 1103 28-29 7.3784177765986705 1103 30-31 6.470623307982958 1103 32-33 8.295169008063084 1103 34-35 7.773550152640855 1103 36-37 4.268348052779789 1103 38-39 6.649171133332182 1103 40-41 7.2753569039503 1103 42-43 7.831695722296761 1103 44-45 5.927347961303379 1103 46-47 6.610544175469553 1103 48-49 6.0525594889520065 1103 50-51 6.784746963947079 1103 52-53 6.365823160076353 1103 54-55 5.0313564133870905 1103 56-57 6.111239696031461 1103 58-59 5.5100537719344835 1103 60-61 4.716897804540562 1103 62-63 2.77703858090074 1103 64-65 5.098324138648117 1103 66-67 4.196549542585686 1103 68-69 5.383401910881318 1103 70-71 3.7057669620302782 1103 72-73 4.199453906011602 1103 74-75 3.547075695352138 1103 76-77 3.8446173373716803 1103 78-79 3.0157360618596094 1103 80-81 3.9869786897101527 1103 82-83 3.214321294854816 1103 84-85 3.2533972734497247 1103 86-87 2.944643871926079 1103 88-89 3.3492481550017104 1103 90-91 3.057385063481174 1103 92-93 2.4997842644173787 1103 94-95 3.087444815879959 1103 96-97 3.1958654920040317 1103 98-99 2.926579279525029 1103 100 2.913578919820779 1104 1 6.479462862103567 1104 2 9.645823504483062 1104 3 7.361433920376598 1104 4 12.054908358120798 1104 5 9.53469803190541 1104 6 8.22528919762253 1104 7 3.827221215186949 1104 8 6.580205491611743 1104 9 6.31684446241205 1104 10-11 6.952015916616094 1104 12-13 7.382868888162346 1104 14-15 7.309689302569733 1104 16-17 6.998329721905304 1104 18-19 6.673016064107941 1104 20-21 7.380330343041006 1104 22-23 6.37778338890665 1104 24-25 6.034490661094953 1104 26-27 8.363298171741757 1104 28-29 7.029051200064476 1104 30-31 6.109418501062347 1104 32-33 7.738851133995674 1104 34-35 7.78689380155723 1104 36-37 6.442815031247303 1104 38-39 7.33066054941375 1104 40-41 7.254800757821734 1104 42-43 7.132479891105493 1104 44-45 6.548780986254499 1104 46-47 6.737165379771817 1104 48-49 5.113984146397051 1104 50-51 5.413924001817371 1104 52-53 5.87028447338411 1104 54-55 4.591459200222435 1104 56-57 5.55424827028331 1104 58-59 3.595672975523719 1104 60-61 4.114678299135054 1104 62-63 3.6636104429638685 1104 64-65 2.6581147719250673 1104 66-67 3.2051543179246593 1104 68-69 4.174187398304827 1104 70-71 2.1822553281037855 1104 72-73 2.0419478135229845 1104 74-75 2.766015398842889 1104 76-77 3.2283391194225644 1104 78-79 4.124673176958396 1104 80-81 3.0904215765974286 1104 82-83 2.677997527718926 1104 84-85 2.799634256198665 1104 86-87 0.2542183988933786 1104 88-89 -0.10280839566483735 1104 90-91 1.900247694752533 1104 92-93 1.9786693349307107 1104 94-95 1.9185306707248948 1104 96-97 2.5533578285731267 1104 98-99 2.4523333725215544 1104 100 0.6613962867912697 1105 1 5.86366839740062 1105 2 -2.5214069243346344 1105 3 0.46159294692555974 1105 4 8.73427596688942 1105 5 8.615537613874146 1105 6 8.45949000774548 1105 7 5.384863451074157 1105 8 5.953590378223858 1105 9 4.822112216846378 1105 10-11 4.758372579274507 1105 12-13 5.381821203008844 1105 14-15 5.957453643473119 1105 16-17 6.2867103558607305 1105 18-19 6.546907684907829 1105 20-21 7.448107262683724 1105 22-23 1.843372715878921 1105 24-25 3.987862968967743 1105 26-27 7.169314295991025 1105 28-29 6.195532933193167 1105 30-31 5.340647339614552 1105 32-33 7.1649485964142485 1105 34-35 6.229189649595632 1105 36-37 6.329656887817077 1105 38-39 3.9731937967163233 1105 40-41 5.37066121202502 1105 42-43 5.744491772474301 1105 44-45 6.518820938416187 1105 46-47 5.3547977742619395 1105 48-49 4.891768984779802 1105 50-51 6.100864109420989 1105 52-53 5.521053373265815 1105 54-55 5.02426478049011 1105 56-57 4.754722742937588 1105 58-59 1.5548987964819823 1105 60-61 4.081169482611937 1105 62-63 -0.34061125568733885 1105 64-65 -0.1591928890485761 1105 66-67 1.010456777948054 1105 68-69 2.4692590417203775 1105 70-71 2.9402090996074044 1105 72-73 2.3493920809091833 1105 74-75 2.45753485189584 1105 76-77 1.8853786512066932 1105 78-79 2.870895481735385 1105 80-81 1.2730185974566268 1105 82-83 0.928694083555822 1105 84-85 1.4801961934548693 1105 86-87 -0.5844551958561404 1105 88-89 1.474254305766074 1105 90-91 1.1505866037913002 1105 92-93 1.3995291862669568 1105 94-95 0.7697764873164452 1105 96-97 1.7494826970014685 1105 98-99 1.5941733227280004 1105 100 2.0105459183906262 1106 1 5.878927603796516 1106 2 9.970456761052628 1106 3 6.479837772522 1106 4 11.06188880561455 1106 5 9.453238871932907 1106 6 8.883365960290611 1106 7 5.9973590108194905 1106 8 7.046103586474615 1106 9 6.201607321716757 1106 10-11 5.4106170576872366 1106 12-13 7.432313930635182 1106 14-15 7.246983964987738 1106 16-17 7.136112990920818 1106 18-19 6.311770282131327 1106 20-21 7.473100069872995 1106 22-23 5.632802980976187 1106 24-25 4.854381680723282 1106 26-27 7.232638355018683 1106 28-29 5.650054277758532 1106 30-31 4.97498533581803 1106 32-33 6.860992068315667 1106 34-35 6.539271719967843 1106 36-37 5.416715213073569 1106 38-39 6.6349673429247105 1106 40-41 5.921470584056479 1106 42-43 5.7114976665607635 1106 44-45 3.9470571808597157 1106 46-47 5.493082976038362 1106 48-49 5.18808948035241 1106 50-51 5.932689363499996 1106 52-53 5.164611427686864 1106 54-55 4.910621183859721 1106 56-57 4.145803898737885 1106 58-59 4.244432171927418 1106 60-61 4.30209123929723 1106 62-63 4.0705069008957295 1106 64-65 4.66723534598837 1106 66-67 2.340241925878857 1106 68-69 2.7054432194655824 1106 70-71 2.1738617294436615 1106 72-73 2.001734204741254 1106 74-75 1.9406667061622613 1106 76-77 2.932334846587544 1106 78-79 3.2640175473498907 1106 80-81 3.0195439474933536 1106 82-83 1.5415239683583373 1106 84-85 2.0807709753801014 1106 86-87 2.4743209172614584 1106 88-89 2.6040692697055796 1106 90-91 2.4699277101822155 1106 92-93 2.0788584144743467 1106 94-95 1.1307864704555328 1106 96-97 0.9323118906512207 1106 98-99 1.3842593792835807 1106 100 2.3203937201230076 1107 1 6.142051593430875 1107 2 10.356051282535336 1107 3 6.084794758362666 1107 4 11.324149262645072 1107 5 9.627466119920296 1107 6 8.433151904936793 1107 7 4.192562058772765 1107 8 6.739118151986926 1107 9 5.983465013493294 1107 10-11 4.932701721250712 1107 12-13 6.561929118410877 1107 14-15 6.438539542206076 1107 16-17 7.399070041749834 1107 18-19 6.710857195916162 1107 20-21 5.746312687359737 1107 22-23 5.7736733373560085 1107 24-25 4.672036982553518 1107 26-27 7.442822028322286 1107 28-29 6.761248113614226 1107 30-31 5.142849006522095 1107 32-33 5.239407814991111 1107 34-35 7.005944479291639 1107 36-37 6.860015321116688 1107 38-39 6.713760834342189 1107 40-41 7.792121442308783 1107 42-43 6.668671567242331 1107 44-45 4.436109082174919 1107 46-47 3.34584416824951 1107 48-49 4.228874539532416 1107 50-51 4.999507940417761 1107 52-53 3.875451889429037 1107 54-55 5.542222577859672 1107 56-57 5.667405211502409 1107 58-59 2.9187110044118363 1107 60-61 2.8765975309811616 1107 62-63 0.45808316065413734 1107 64-65 2.287834619743638 1107 66-67 4.2471359705096 1107 68-69 5.031430839186292 1107 70-71 4.320697609507679 1107 72-73 2.2931459952513293 1107 74-75 2.453343494763306 1107 76-77 3.025288079351416 1107 78-79 3.728668763038076 1107 80-81 2.1348868828543246 1107 82-83 1.5303947146682155 1107 84-85 2.1534774961479357 1107 86-87 2.3838880136635403 1107 88-89 2.436491141532322 1107 90-91 1.4325541328176534 1107 92-93 2.3440900572984127 1107 94-95 2.214462617296693 1107 96-97 2.2806047281234996 1107 98-99 1.7779546976858427 1107 100 0.24543535328006172 1108 1 4.360618597935378 1108 2 -3.201048004251451 1108 3 -0.11686627767337043 1108 4 6.543391942825249 1108 5 7.006074415716096 1108 6 6.76555618421607 1108 7 4.1744783500640565 1108 8 4.92508380514008 1108 9 4.905659082562362 1108 10-11 3.2813644464759335 1108 12-13 2.3424987505430046 1108 14-15 3.5364350940078744 1108 16-17 4.388231077785868 1108 18-19 3.5382619444146606 1108 20-21 4.654470063485864 1108 22-23 4.148204118136796 1108 24-25 2.8206903233943628 1108 26-27 4.568199749793759 1108 28-29 5.987538466467075 1108 30-31 4.284562595110689 1108 32-33 4.297002128054174 1108 34-35 2.838135263826171 1108 36-37 1.114383752047626 1108 38-39 2.2650136496575044 1108 40-41 5.311371160777249 1108 42-43 3.010464923098187 1108 44-45 3.610639862955697 1108 46-47 4.785436885967229 1108 48-49 4.755078086829716 1108 50-51 1.96155279777512 1108 52-53 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2213 54-55 -17.543538793267555 2213 56-57 -17.496251127763685 2213 58-59 -18.035332063045864 2213 60-61 -19.06421767446819 2213 62-63 -17.21862525507867 2213 64-65 -17.52610737102762 2213 66-67 -8.747612436433926 2213 68-69 -22.448298890543438 2213 70-71 -16.92489676535499 2213 72-73 -9.16166613395104 2213 74-75 -3.9393174588732585 2213 76-77 -2.9042109318549123 2213 78-79 -4.535328160714943 2213 80-81 -2.808334387196215 2213 82-83 -2.90219153011315 2213 84-85 -2.730844630857117 2213 86-87 -3.250104515450939 2213 88-89 -2.5357017830996256 2213 90-91 -2.930596823016227 2213 92-93 -3.1310912215414 2213 94-95 -3.2972271212223134 2213 96-97 -3.5858092468270613 2213 98-99 -3.5432054868889757 2213 100 -3.5297349389634007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 123.0 3 130.0 4 699.0 5 2406.0 6 3929.0 7 4209.0 8 3855.0 9 3899.0 10 4705.0 11 5891.0 12 7392.0 13 9482.0 14 8233.0 15 9346.0 16 9856.0 17 10277.0 18 10303.0 19 10535.0 20 10956.0 21 11243.0 22 11957.0 23 13066.0 24 14001.0 25 15078.0 26 16135.0 27 17028.0 28 17965.0 29 19050.0 30 20768.0 31 23087.0 32 26670.0 33 31388.0 34 36247.0 35 40408.0 36 39192.0 37 28327.0 38 7678.0 39 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32641093511954 21.153813418671753 12.724587847786676 22.795187798422035 2 13.582037176562872 24.494008607788295 36.3637771057602 25.560177109888638 3 20.222039836188422 31.46005151122689 27.28289823069221 21.03501042189248 4 13.335207040325098 22.176869345036266 31.91507466034763 32.572848954291004 5 12.22986721345678 41.03797394521181 33.02860552817368 13.703553313157734 6 30.266366413273634 34.24190357180048 18.623737373737374 16.86799264118852 7 22.877690454901323 35.74909195141673 22.423773118339373 18.949444475342577 8 25.53579719064123 35.73888726935078 21.618242477245488 17.107073062762503 9 25.481190851243348 20.387072372932778 20.17925943782445 33.952477337999426 10-11 21.899198363515016 31.98983331588116 24.27258813611849 21.838380184485334 12-13 24.841067169730294 29.71394829356041 24.819485389573043 20.62549914713625 14-15 24.83726498852721 29.131746777729813 23.107946317116895 22.923041916626087 16-17 20.786185559961712 32.66101463147819 27.38690944208943 19.16589036647067 18-19 22.379548292219813 31.191370548179098 27.959766438976736 18.469314720624354 20-21 22.50097649371522 30.541948675917208 26.49222113367185 20.464853696695716 22-23 20.673818938627395 31.225704650162655 24.31789906109427 23.782577350115677 24-25 21.68128360967813 31.704300475862507 22.960392026515763 23.654023887943602 26-27 23.14187737842632 28.724048711041117 24.235232956627943 23.898840953904614 28-29 22.861442930063674 27.9889491132979 27.030258380255752 22.119349576382678 30-31 24.690421229201185 30.15803956298643 25.005283793856776 20.146255413955608 32-33 19.73142873816247 30.13245927717458 27.44858381937037 22.687528165292576 34-35 18.62136867959685 30.485093588549166 27.554734479546035 23.33880325230795 36-37 23.403083176397068 30.034270439340478 25.05580445653174 21.50684192773072 38-39 24.37287020921431 27.96704597101704 25.02089210875206 22.639191711016583 40-41 21.764377861899604 30.307985973340767 24.758018680764817 23.16961748399481 42-43 24.00593368641858 29.12215787272285 25.302031170817894 21.569877270040678 44-45 25.44778424816589 27.565499545018003 24.84979289387466 22.13692331294145 46-47 21.972750273702076 29.48648776067176 28.479578420228286 20.061183545397874 48-49 23.170855680043374 28.68870891722296 27.907177337124445 20.23325806560922 50-51 21.394480488693997 29.26379825147344 27.304449702994916 22.03727155683765 52-53 20.02539174881796 29.885303259916263 24.978952120334988 25.110352870930786 54-55 21.46887381500612 30.7888895803514 23.19372705208787 24.54850955255461 56-57 23.026985888170444 28.05014368276665 24.115748644604633 24.80712178445827 58-59 22.476074694435574 26.8296002488723 27.031808990511742 23.662516066180384 60-61 24.129519151196256 28.658551648324504 25.330303067732203 21.88162613274704 62-63 19.81523109372259 29.251439108670553 27.097671724115425 23.835658073491434 64-65 18.711708091568987 29.544231383069388 27.42080270066742 24.32325782469421 66-67 22.55286198694266 28.80236854091806 25.234070550129058 23.410698922010223 68-69 24.134369609941604 27.147503169094573 24.816400324182787 23.90172689678103 70-71 21.625389394695627 29.05722191119017 24.891192213422208 24.426196480692 72-73 23.606562311860323 28.03833032309854 25.525185631145895 22.829921733895244 74-75 24.7915707483522 26.74950022821085 24.59176168808261 23.867167335354345 76-77 21.696381803860103 28.480349624345752 27.80041343958544 22.0228551322087 78-79 22.949084461171022 27.887545937773915 27.769836674627875 21.393532926427188 80-81 21.341772356312212 28.174171422111964 27.208784066112635 23.275272155463195 82-83 20.002131584504376 29.02859510651946 25.384980098570843 25.584293210405317 84-85 21.173116888444632 29.17073151208247 23.853063217476482 25.803088381996414 86-87 22.544564802746606 27.13177909527291 24.859232444289027 25.464423657691455 88-89 22.3699846305403 26.02528639688268 27.21310756928459 24.391621403292433 90-91 23.569033256607668 27.538594332883566 25.797979998412572 23.094392412096198 92-93 20.092947589264902 28.100625437086784 27.152302114384064 24.654124859264257 94-95 19.0662441629078 28.315537946814622 27.397506724210935 25.22071116606664 96-97 22.433358020150408 27.755769507970196 25.794608995611046 24.01626347626835 98-99 23.31630670973498 26.418258793418136 25.433026350695453 24.832408146151426 100 21.048629956148275 27.977162958095697 26.644538981186834 24.329668104569194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1765.0 1 1770.5 2 1524.0 3 1060.5 4 705.0 5 479.5 6 377.0 7 358.5 8 382.0 9 426.0 10 486.5 11 583.0 12 720.5 13 852.0 14 1020.5 15 1216.0 16 1391.5 17 1550.0 18 1726.0 19 1875.0 20 1925.0 21 2030.0 22 2286.5 23 2605.5 24 2926.5 25 3355.0 26 4015.5 27 4709.5 28 5428.0 29 6173.0 30 7077.0 31 7976.0 32 8677.5 33 9391.0 34 9900.0 35 10363.5 36 10939.5 37 11678.5 38 12383.0 39 12857.0 40 13164.0 41 13228.0 42 13184.5 43 13217.5 44 13204.0 45 13371.5 46 14628.0 47 17236.5 48 21385.0 49 29125.5 50 39525.5 51 38537.5 52 25898.0 53 16457.5 54 11846.5 55 9407.0 56 7889.5 57 6685.0 58 5613.5 59 4770.5 60 4042.5 61 3387.5 62 2811.5 63 2327.5 64 1948.5 65 1526.0 66 1205.0 67 916.5 68 693.0 69 562.5 70 449.5 71 339.0 72 253.0 73 185.5 74 131.0 75 92.0 76 64.5 77 50.5 78 34.0 79 19.0 80 15.5 81 12.0 82 6.5 83 3.0 84 3.0 85 3.5 86 2.0 87 1.5 88 2.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.037300044110461 2 23.33957737034392 3 5.765613224435217 4 23.28676349869152 5 11.827933592985051 6 8.823081442154965 7 7.529042684289752 8 8.397603397494604 9 4.815952558505704 10-11 6.493633653711115 12-13 9.721213967389906 14-15 6.983694953407088 16-17 7.421041283832032 18-19 6.450512215433123 20-21 7.3258971929526115 22-23 5.480279854178329 24-25 5.080318623911826 26-27 9.062919716980945 28-29 6.027902339832539 30-31 4.069931895820187 32-33 8.48157151927904 34-35 6.580766651699439 36-37 6.466732205978055 38-39 5.557522614029501 40-41 7.772540347226481 42-43 6.126112404534476 44-45 5.875988282045855 46-47 5.597182468959487 48-49 3.6832235846574712 50-51 6.12759594025505 52-53 4.2594288585280555 54-55 4.644258024444713 56-57 4.355364168458449 58-59 3.3518017046814452 60-61 3.9131716213462986 62-63 3.0293799414102294 64-65 4.2568573966123955 66-67 2.289392323988377 68-69 4.814370120403759 70-71 2.309073897881313 72-73 1.4338867251245675 74-75 0.7556141936785554 76-77 1.059244504489179 78-79 1.6095373544404201 80-81 2.008113951367721 82-83 0.7071520268065015 84-85 1.154685302512714 86-87 0.44248925425626395 88-89 0.7080421482388453 90-91 0.3143117679987499 92-93 0.2970038512587306 94-95 0.49767678306158253 96-97 0.5106329950212541 98-99 0.4584125376570817 100 0.3572354015139977 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 505549.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.56071321876654 #Duplication Level Percentage of deduplicated Percentage of total 1 95.24349446359992 74.82396854508052 2 3.176428082994229 4.990849113762919 3 0.6987812912381258 1.6469026987079332 4 0.26595262329055286 0.8357371107243109 5 0.14001120309803533 0.5499689986999615 6 0.08466415653920317 0.3990765913071058 7 0.04655932051892962 0.2560413398863784 8 0.039000236981396944 0.2451109146367567 9 0.030324492297075283 0.21440823685697036 >10 0.22464452857435183 3.467645089981291 >50 0.027137414116006404 1.4403755987864348 >100 0.01576554372899828 2.4245170043217477 >500 0.003876773048114248 2.2545713772706124 >1k 0.002842966901950448 3.956261102856437 >5k 5.169030730818997E-4 2.4945662771205637 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6873 1.3595121343331704 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5413 1.0707171807282776 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2494 0.4933250782812349 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2487 0.4919404449420333 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2174 0.43002755420345007 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1891 0.3740488063471592 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1781 0.3522902824454207 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1682 0.3327076109338561 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1610 0.3184656680163545 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1597 0.31589420610069446 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1459 0.2885971488421498 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1302 0.2575418010914867 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1008 0.19938720084502196 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 967 0.19127720557255576 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 922 0.1823759912491173 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 906 0.17921111504522805 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 883 0.17466160550213727 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 754 0.1491447911082803 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 750 0.148353572057308 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 725 0.14340845298873106 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 723 0.14301284346324492 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 717 0.14182601488678645 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 710 0.1404413815475849 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 679 0.1343094339025495 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 663 0.13114455769866026 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 595 0.11769383383213101 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 570 0.11274871476355407 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 540 0.10681457188126177 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.9780476274307732E-4 0.0 9 0.0 0.0 0.0 1.9780476274307732E-4 0.0 10-11 1.9780476274307732E-4 0.0 0.0 1.9780476274307732E-4 0.0 12-13 1.9780476274307732E-4 0.0 0.0 3.9560952548615464E-4 0.0 14-15 1.9780476274307732E-4 0.0 0.0 3.9560952548615464E-4 0.0 16-17 1.9780476274307732E-4 0.0 0.0 3.9560952548615464E-4 0.0 18-19 1.9780476274307732E-4 0.0 0.0 3.9560952548615464E-4 0.0 20-21 1.9780476274307732E-4 0.0 0.0 4.945119068576933E-4 0.0 22-23 1.9780476274307732E-4 0.0 0.0 0.0011868285764584639 0.0 24-25 1.9780476274307732E-4 0.0 0.0 0.001780242864687696 0.0 26-27 1.9780476274307732E-4 0.0 0.0 0.00296707144114616 0.0 28-29 1.9780476274307732E-4 0.0 0.0 0.005736338119549243 0.0 30-31 1.9780476274307732E-4 0.0 0.0 0.016714502451790035 0.0 32-33 1.9780476274307732E-4 0.0 0.0 0.041637902557417776 0.0 34-35 1.9780476274307732E-4 0.0 0.0 0.078033978902144 0.0 36-37 1.9780476274307732E-4 0.0 0.0 0.1338149219956918 0.0 38-39 1.9780476274307732E-4 0.0 0.0 0.2179808485428712 0.0 40-41 1.9780476274307732E-4 0.0 0.0 0.3598068634296576 0.0 42-43 1.9780476274307732E-4 0.0 0.0 0.45851144003845323 0.0 44-45 1.9780476274307732E-4 0.0 0.0 0.5622600380971974 0.0 46-47 1.9780476274307732E-4 0.0 0.0 0.6919210600752845 0.0 48-49 1.9780476274307732E-4 0.0 0.0 0.8050653843643247 0.0 50-51 1.9780476274307732E-4 0.0 0.0 0.9232537301033135 0.0 52-53 1.9780476274307732E-4 0.0 0.0 1.0810030283909176 0.0 54-55 1.9780476274307732E-4 0.0 0.0 1.238257814771664 0.0 56-57 1.9780476274307732E-4 0.0 0.0 1.4083699107307106 0.0 58-59 1.9780476274307732E-4 0.0 0.0 1.6039988210836142 0.0 60-61 1.9780476274307732E-4 0.0 0.0 1.7887484694856481 0.0 62-63 1.9780476274307732E-4 0.0 0.0 1.986454329847354 0.0 64-65 1.9780476274307732E-4 0.0 0.0 2.228270652300766 0.0 66-67 1.9780476274307732E-4 0.0 0.0 2.4850212343412803 0.0 68-69 1.9780476274307732E-4 0.0 0.0 2.797948369000829 0.0 70-71 1.9780476274307732E-4 0.0 0.0 3.302746123521162 0.0 72-73 1.9780476274307732E-4 0.0 0.0 3.7045864990337236 0.0 74-75 1.9780476274307732E-4 0.0 0.0 4.071118724396646 0.0 76-77 1.9780476274307732E-4 0.0 0.0 4.5061903000500445 0.0 78-79 1.9780476274307732E-4 0.0 0.0 4.932360661380004 0.0 80-81 1.9780476274307732E-4 0.0 0.0 5.418564768202488 1.9780476274307732E-4 82-83 1.9780476274307732E-4 0.0 0.0 6.055397201853826 1.9780476274307732E-4 84-85 1.9780476274307732E-4 0.0 0.0 6.642481737675279 1.9780476274307732E-4 86-87 1.9780476274307732E-4 0.0 0.0 7.220566156791923 1.9780476274307732E-4 88 1.9780476274307732E-4 0.0 0.0 7.738517928034671 1.9780476274307732E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 325 0.0 43.468452 1 TTGTTCG 70 3.1002328E-6 42.70423 5 GTACAAA 245 0.0 37.605804 1 TGTTCGC 85 1.1294393E-5 35.37402 6 GGTATGG 105 1.19091E-5 35.09875 1 TACATCG 130 1.3536992E-6 33.064335 2 TCGCTAT 95 2.400535E-5 31.683817 9 TACATAA 235 7.2759576E-12 31.35584 2 TAGGTGT 80 3.2798416E-4 31.320745 6 CATAGTC 65 0.0045178533 30.806433 7 GGATATA 80 0.0045854994 30.711409 1 GTATAAG 100 3.6788307E-4 30.711407 1 ATAGGTG 100 3.5718986E-5 29.892958 5 GTACATC 190 2.7181159E-8 29.095016 1 ACGACTG 105 4.753573E-5 28.66631 9 AGCGTTC 35 0.007597796 27.700651 38-39 TATAGGT 105 8.6736894E-4 26.524233 4 CACGGGA 110 0.0011378812 25.318583 4 TTCGCTA 120 1.1821794E-4 25.074205 8 GTTCGCT 120 1.1963628E-4 25.030228 7 >>END_MODULE