##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295142_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 505549 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59086062874222 33.0 33.0 33.0 27.0 33.0 2 30.19777113593341 33.0 33.0 33.0 27.0 33.0 3 30.981740642351188 33.0 33.0 33.0 27.0 33.0 4 35.23609383066726 37.0 37.0 37.0 33.0 37.0 5 35.63894696656506 37.0 37.0 37.0 33.0 37.0 6 35.47747696069026 37.0 37.0 37.0 33.0 37.0 7 35.385165434013324 37.0 37.0 37.0 33.0 37.0 8 35.40989300740383 37.0 37.0 37.0 33.0 37.0 9 35.29881574288546 37.0 37.0 37.0 33.0 37.0 10-11 35.29558559110986 37.0 37.0 37.0 33.0 37.0 12-13 35.211900330136146 37.0 37.0 37.0 33.0 37.0 14-15 36.60554466530445 40.0 37.0 40.0 33.0 40.0 16-17 36.566540533162964 37.0 37.0 40.0 33.0 40.0 18-19 36.6652342305098 40.0 37.0 40.0 33.0 40.0 20-21 36.71376266197737 40.0 37.0 40.0 33.0 40.0 22-23 36.728731537397955 40.0 37.0 40.0 33.0 40.0 24-25 36.70188547499846 40.0 37.0 40.0 33.0 40.0 26-27 36.56465050865495 40.0 37.0 40.0 33.0 40.0 28-29 36.46062894002362 38.5 37.0 40.0 33.0 40.0 30-31 36.19041180973555 37.0 37.0 40.0 30.0 40.0 32-33 36.05371783941814 37.0 37.0 40.0 27.0 40.0 34-35 36.02646133213595 37.0 37.0 40.0 27.0 40.0 36-37 35.97071401585207 37.0 37.0 40.0 27.0 40.0 38-39 35.83132099954703 37.0 37.0 40.0 27.0 40.0 40-41 35.655329157015444 37.0 37.0 40.0 27.0 40.0 42-43 35.41828784153465 37.0 37.0 40.0 27.0 40.0 44-45 35.1288618907366 37.0 35.0 40.0 24.5 40.0 46-47 34.77384289158914 37.0 33.0 40.0 22.0 40.0 48-49 34.48498167338873 37.0 33.0 40.0 22.0 40.0 50-51 34.356377917867505 37.0 33.0 40.0 22.0 40.0 52-53 34.34667757230258 37.0 33.0 40.0 22.0 40.0 54-55 34.21422849219364 37.0 33.0 40.0 22.0 40.0 56-57 33.97971215450926 37.0 33.0 40.0 22.0 40.0 58-59 33.88312112179037 37.0 33.0 40.0 22.0 40.0 60-61 33.62825858621024 37.0 33.0 40.0 22.0 40.0 62-63 33.373577041988014 37.0 33.0 40.0 22.0 40.0 64-65 33.26821435706529 37.0 33.0 37.0 22.0 40.0 66-67 32.9975264514419 37.0 33.0 37.0 18.5 40.0 68-69 32.766678403082594 37.0 33.0 37.0 15.0 40.0 70-71 32.408661672755755 37.0 33.0 37.0 15.0 40.0 72-73 32.089399840569364 37.0 33.0 37.0 15.0 37.0 74-75 31.87546706649603 37.0 33.0 37.0 15.0 37.0 76-77 31.538173352137974 33.0 30.0 37.0 15.0 37.0 78-79 31.300460489487666 33.0 27.0 37.0 15.0 37.0 80-81 31.234105892801686 33.0 27.0 37.0 15.0 37.0 82-83 31.15150559095162 33.0 27.0 37.0 15.0 37.0 84-85 30.92615948206801 33.0 27.0 37.0 15.0 37.0 86-87 30.879628878704143 33.0 27.0 37.0 15.0 37.0 88-89 30.92487177306255 33.0 27.0 37.0 15.0 37.0 90-91 30.94997913159753 33.0 27.0 37.0 15.0 37.0 92-93 30.853205129473107 33.0 27.0 37.0 15.0 37.0 94-95 30.97578177387355 33.0 27.0 37.0 15.0 37.0 96-97 30.950843538410716 33.0 27.0 37.0 15.0 37.0 98-99 30.84019649925131 33.0 27.0 37.0 15.0 37.0 100 30.218471404354474 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.574162147841168 1101 2 3.9677322142021616 1101 3 2.229536658033119 1101 4 0.9143868316651123 1101 5 0.46426345146921477 1101 6 0.1784447436594334 1101 7 0.28340099310351263 1101 8 0.3375290730896907 1101 9 0.39721720666871363 1101 10-11 0.509494650526598 1101 12-13 0.4817583522290505 1101 14-15 0.9906566609861613 1101 16-17 0.8865316978732309 1101 18-19 0.798971092754698 1101 20-21 0.7584986809827114 1101 22-23 0.6822564143879362 1101 24-25 0.6256448497192224 1101 26-27 0.7352899870417389 1101 28-29 0.4428402989525395 1101 30-31 0.5612795614023511 1101 32-33 0.6927264056331452 1101 34-35 0.9497525528520967 1101 36-37 0.932225416419243 1101 38-39 0.8520629161987188 1101 40-41 0.8232106386926574 1101 42-43 0.9842461842674055 1101 44-45 0.8301534756825646 1101 46-47 0.8583248174811047 1101 48-49 0.7646594380178939 1101 50-51 0.9565502504899399 1101 52-53 0.7787845935385178 1101 54-55 1.1259175576078064 1101 56-57 0.8175857028305202 1101 58-59 0.8069118561329773 1101 60-61 0.8993495035704839 1101 62-63 0.8429107556417748 1101 64-65 1.0249390991664882 1101 66-67 1.24818540272544 1101 68-69 0.8895979898414907 1101 70-71 0.8830329814091371 1101 72-73 0.9641550170618913 1101 74-75 0.7889085187272755 1101 76-77 1.0123319912782982 1101 78-79 1.1282693648903077 1101 80-81 1.01728736038676 1101 82-83 0.9928558593104917 1101 84-85 1.0510304186047463 1101 86-87 1.1512759748357446 1101 88-89 1.0132079530107028 1101 90-91 0.7662943493782386 1101 92-93 0.9817635986133588 1101 94-95 0.972154005625061 1101 96-97 0.7848298714123381 1101 98-99 0.6779425260580645 1101 100 0.8555209906583698 1102 1 0.6166221990791634 1102 2 4.157639193318559 1102 3 2.359881239399911 1102 4 1.1133149925508334 1102 5 0.5190221895865932 1102 6 0.5639301902656015 1102 7 0.3503069796309788 1102 8 0.4033082946331845 1102 9 0.5138460137180587 1102 10-11 0.5659466747410633 1102 12-13 0.5327366212010389 1102 14-15 0.812381411760029 1102 16-17 0.876085582113042 1102 18-19 0.9672929897373024 1102 20-21 0.7771915237495648 1102 22-23 0.7371712989062971 1102 24-25 0.7791147915370402 1102 26-27 0.7920397879354653 1102 28-29 0.47403426966136664 1102 30-31 0.5726609862578798 1102 32-33 0.6865633307260381 1102 34-35 0.7998276835952893 1102 36-37 0.6575160331190659 1102 38-39 0.5672267718378379 1102 40-41 0.6242687463395171 1102 42-43 0.6978575994324387 1102 44-45 0.9205453717628274 1102 46-47 0.8083465916651633 1102 48-49 0.5736009772761577 1102 50-51 0.496245568844202 1102 52-53 0.6277145198986247 1102 54-55 0.5618994948432317 1102 56-57 0.6039338779184931 1102 58-59 0.6865127804569013 1102 60-61 0.5610434173917938 1102 62-63 0.55680621601865 1102 64-65 0.5616782287483773 1102 66-67 0.29443259267871014 1102 68-69 0.4955135060073488 1102 70-71 0.4866871210542385 1102 72-73 0.5618752011420156 1102 74-75 0.4912075587015394 1102 76-77 0.5273522550009382 1102 78-79 0.5251279974715466 1102 80-81 0.49439229626080916 1102 82-83 0.37973563835156554 1102 84-85 0.350908012380863 1102 86-87 0.2902827581806484 1102 88-89 0.2181810981836989 1102 90-91 0.31137711089403197 1102 92-93 0.5275223406540128 1102 94-95 0.4458099892100691 1102 96-97 0.38723170122845474 1102 98-99 0.48061349263567266 1102 100 0.5345247923132064 1103 1 0.5654755667003251 1103 2 4.098979054117429 1103 3 2.231148544704009 1103 4 0.9028138125756726 1103 5 0.5579258309380393 1103 6 0.4134124786587208 1103 7 0.32296350104024185 1103 8 0.48747426582662 1103 9 0.5599453073302172 1103 10-11 0.6322169549402403 1103 12-13 0.6713275990829857 1103 14-15 0.825997646301559 1103 16-17 0.7755105688358199 1103 18-19 0.8184208543216727 1103 20-21 0.8588310303562281 1103 22-23 0.7305518578671126 1103 24-25 0.6430211282632143 1103 26-27 0.660729516361485 1103 28-29 0.7051995899717127 1103 30-31 0.8054535142797903 1103 32-33 0.8948814568216505 1103 34-35 1.0701785614888237 1103 36-37 0.8887188890183353 1103 38-39 0.8639619742305342 1103 40-41 0.8841951126948473 1103 42-43 0.9156650418612315 1103 44-45 0.8497991722412053 1103 46-47 0.8391633101761968 1103 48-49 0.7005898643459432 1103 50-51 0.6254602364745523 1103 52-53 0.6949255857096901 1103 54-55 0.7916851826030324 1103 56-57 0.7767373471652661 1103 58-59 0.8419296850486191 1103 60-61 0.6369711254447736 1103 62-63 0.8762356750669156 1103 64-65 0.8999321849595248 1103 66-67 0.8396913286877279 1103 68-69 0.9378716194846248 1103 70-71 0.886324143922991 1103 72-73 0.710025774757689 1103 74-75 0.7759098096389394 1103 76-77 0.659353740451909 1103 78-79 0.8554016478874331 1103 80-81 0.8466805695837891 1103 82-83 0.8537965498274751 1103 84-85 0.6490863383734577 1103 86-87 0.7013920945594236 1103 88-89 0.7749081545549679 1103 90-91 0.6468230856014365 1103 92-93 0.7688905528019667 1103 94-95 0.8062886079003952 1103 96-97 0.7284617504639677 1103 98-99 0.8319166564066443 1103 100 0.7367633509465925 1104 1 0.3242537154628593 1104 2 3.5025183138373315 1104 3 2.035829952724118 1104 4 0.975089367248529 1104 5 0.3107881203463023 1104 6 0.2563044124805387 1104 7 0.30858408836103024 1104 8 0.39817962705380694 1104 9 0.3435809724411385 1104 10-11 0.501635429547143 1104 12-13 0.29183270993615906 1104 14-15 0.3879513775743817 1104 16-17 0.38503902568211856 1104 18-19 0.4965836169661344 1104 20-21 0.5370401887055891 1104 22-23 0.4758146122091915 1104 24-25 0.4681569374450021 1104 26-27 0.5054984413323069 1104 28-29 0.4338066932355247 1104 30-31 0.313069328540692 1104 32-33 0.35963618633468997 1104 34-35 0.48559267978321685 1104 36-37 0.4331151018264876 1104 38-39 0.36459808376346814 1104 40-41 0.43421578727211596 1104 42-43 0.5745654473759743 1104 44-45 0.5481627875898809 1104 46-47 0.3826438182824248 1104 48-49 0.3159137145057187 1104 50-51 0.4246311370884399 1104 52-53 0.3536013383375618 1104 54-55 0.49882409785517723 1104 56-57 0.4286340361398615 1104 58-59 0.39290025063667855 1104 60-61 0.49533236403102165 1104 62-63 0.48263974747026595 1104 64-65 0.5054575878288858 1104 66-67 0.5268552650621174 1104 68-69 0.5553133928749787 1104 70-71 0.4770733287423141 1104 72-73 0.2789872923693366 1104 74-75 0.47445331008905356 1104 76-77 0.5486055649426547 1104 78-79 0.32776242414455226 1104 80-81 0.38287328920232966 1104 82-83 0.44865948714769033 1104 84-85 0.3766804687470149 1104 86-87 0.49781343918834864 1104 88-89 0.3840524224063273 1104 90-91 0.49307016145109017 1104 92-93 0.5023639143417178 1104 94-95 0.44024928713351485 1104 96-97 0.3688669558992608 1104 98-99 0.5707320356874597 1104 100 0.5042012173302162 1105 1 0.48348230635076916 1105 2 3.6257622599116885 1105 3 2.0341272606783782 1105 4 0.9826610513983454 1105 5 0.3573490082879829 1105 6 0.2634377959984775 1105 7 0.2993993275363067 1105 8 0.23860560964561728 1105 9 0.3600343545252329 1105 10-11 0.3383342273976737 1105 12-13 0.39118845688842896 1105 14-15 0.5324724567389723 1105 16-17 0.40971746943788645 1105 18-19 0.5030791019808163 1105 20-21 0.4816554454780615 1105 22-23 0.4152533592885419 1105 24-25 0.49916467043682644 1105 26-27 0.47043439078271376 1105 28-29 0.4475960547504485 1105 30-31 0.37034169735193956 1105 32-33 0.4159627056410997 1105 34-35 0.5249533800214294 1105 36-37 0.5960171082331698 1105 38-39 0.26766521056433135 1105 40-41 0.24322832364781277 1105 42-43 0.5868598615684419 1105 44-45 0.4965663304928327 1105 46-47 0.4045197449868212 1105 48-49 0.4171570870417085 1105 50-51 0.39132762123303877 1105 52-53 0.3191523751059293 1105 54-55 0.25367147473180296 1105 56-57 0.34049894830050675 1105 58-59 0.3245734184911555 1105 60-61 0.3741414246338266 1105 62-63 0.32987161013389965 1105 64-65 0.29340888268953336 1105 66-67 0.16021678650300686 1105 68-69 0.19503190284834915 1105 70-71 0.2594195895002329 1105 72-73 0.3861412059540541 1105 74-75 0.4425828254817148 1105 76-77 0.4284957127917224 1105 78-79 0.1530002307513243 1105 80-81 0.400067220123546 1105 82-83 0.34693626503517905 1105 84-85 0.4114561207395724 1105 86-87 0.5074430181264553 1105 88-89 0.5253815649990443 1105 90-91 0.33476623825309204 1105 92-93 0.061545905236794596 1105 94-95 0.3522518810911599 1105 96-97 0.18167348120175575 1105 98-99 0.08055713907101136 1105 100 0.32928830717695945 1106 1 0.21475407565361948 1106 2 3.7091779690103124 1106 3 2.13483625482608 1106 4 0.7894147390197972 1106 5 0.32919658820855346 1106 6 0.0880722485170864 1106 7 0.2326604916625854 1106 8 -0.02039665508517885 1106 9 0.0620181236866415 1106 10-11 0.08549929557266722 1106 12-13 0.03794108367075921 1106 14-15 0.030511913525046452 1106 16-17 0.1859001041443591 1106 18-19 -0.06290308235124087 1106 20-21 0.12287603732985986 1106 22-23 -0.08658864318996962 1106 24-25 -0.12947412830752825 1106 26-27 0.005043463528956238 1106 28-29 -0.05398555994313625 1106 30-31 0.05095719299686863 1106 32-33 -0.06621479524694962 1106 34-35 0.16945976032582877 1106 36-37 0.07874632583217078 1106 38-39 -0.08881490914976098 1106 40-41 -0.11337596246371362 1106 42-43 5.417127005671318E-4 1106 44-45 -0.05784868906036422 1106 46-47 -0.2022083056891404 1106 48-49 -0.05970054674055092 1106 50-51 -0.1056084021311392 1106 52-53 -0.22901092661118838 1106 54-55 -0.21022583925568483 1106 56-57 -0.08614161529439457 1106 58-59 -0.08560163603758042 1106 60-61 -0.03212620578911185 1106 62-63 -0.042417825355030914 1106 64-65 -0.1508054453894374 1106 66-67 -0.39582364754092936 1106 68-69 -0.23708443905688625 1106 70-71 -0.15643215983955372 1106 72-73 -0.2607396055557345 1106 74-75 -0.24924823106583105 1106 76-77 -0.26789413915692606 1106 78-79 -0.2458801017735226 1106 80-81 -0.22491771113890024 1106 82-83 -0.17394137038656154 1106 84-85 -0.36100875503777274 1106 86-87 -0.18323558137302243 1106 88-89 -0.24183095161674828 1106 90-91 -0.2483878775616084 1106 92-93 -0.30321920730055396 1106 94-95 -0.5314110356699366 1106 96-97 -0.30981609766118723 1106 98-99 -0.2509104836367335 1106 100 -0.261990470093437 1107 1 0.4610127779988886 1107 2 3.512186116923779 1107 3 2.1502904827239533 1107 4 0.8943920337175584 1107 5 0.3042199433942159 1107 6 0.2805019189449567 1107 7 0.11692112741104665 1107 8 0.14243082523553596 1107 9 0.14832264281351826 1107 10-11 0.17269131175475394 1107 12-13 0.09645415038941252 1107 14-15 0.22207114633765457 1107 16-17 -0.04787514991944164 1107 18-19 0.20348860239030842 1107 20-21 0.274161587984203 1107 22-23 0.1612330287976249 1107 24-25 0.34005689956434537 1107 26-27 0.29032888420661607 1107 28-29 0.10753727939755464 1107 30-31 -0.08016897374697152 1107 32-33 0.19307163275002637 1107 34-35 0.27949883456688696 1107 36-37 0.0857025155656359 1107 38-39 0.04766954645101862 1107 40-41 0.06289638743352555 1107 42-43 0.06120345650028014 1107 44-45 -0.07340283653515911 1107 46-47 -0.05291870951045752 1107 48-49 0.0585623960940822 1107 50-51 0.1425248077243424 1107 52-53 0.03138680204623512 1107 54-55 0.05252487358519886 1107 56-57 0.16144609865941817 1107 58-59 0.06746256432148101 1107 60-61 0.11748102091460311 1107 62-63 0.30032013352359144 1107 64-65 0.19610435891442535 1107 66-67 0.09931684913247807 1107 68-69 0.35628593763179595 1107 70-71 0.16152249454432166 1107 72-73 0.20535881928930877 1107 74-75 0.11338062863246279 1107 76-77 0.28926798238752127 1107 78-79 0.12456644937636696 1107 80-81 0.227939745054897 1107 82-83 0.48076581651178785 1107 84-85 0.36118151458261494 1107 86-87 0.36180540190060384 1107 88-89 0.31536325792191633 1107 90-91 0.3876688735086695 1107 92-93 0.3667923281302805 1107 94-95 0.2805411329098213 1107 96-97 0.17535031311388138 1107 98-99 -0.0035157038253608164 1107 100 0.10934804606022652 1108 1 0.3101775677886778 1108 2 3.443187430737595 1108 3 2.0511819992404696 1108 4 0.840253520204044 1108 5 0.1849819554062293 1108 6 0.022680034560572437 1108 7 0.09877172801164846 1108 8 0.0010926120223260227 1108 9 0.14996491008079005 1108 10-11 0.05613725770070488 1108 12-13 0.08944166915818386 1108 14-15 0.10310061330461906 1108 16-17 0.24484725999047185 1108 18-19 -0.021778852564636964 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56-57 0.3247164486554297 2210 58-59 0.19555962471942934 2210 60-61 0.5224137665553101 2210 62-63 0.5060717764433775 2210 64-65 -0.07776211185471027 2210 66-67 0.040089733268196426 2210 68-69 0.6265368459358029 2210 70-71 0.5096438143470507 2210 72-73 0.3487142480429739 2210 74-75 -0.4265363427004374 2210 76-77 0.25791412536994685 2210 78-79 0.577478147420809 2210 80-81 0.5833946943151531 2210 82-83 0.18504367211707873 2210 84-85 0.4585596765625475 2210 86-87 0.27116862099833483 2210 88-89 0.5104925844671726 2210 90-91 0.6475514488779659 2210 92-93 0.6883461828699744 2210 94-95 0.3271522193439189 2210 96-97 0.14967862372537866 2210 98-99 0.325057433154857 2210 100 0.4332441292483473 2211 1 0.12027330035089179 2211 2 3.653900386398302 2211 3 1.782720073197705 2211 4 0.7968616958086727 2211 5 0.3890325162194017 2211 6 0.1834291363832108 2211 7 0.24461601472738437 2211 8 -0.02321842022443832 2211 9 -0.1241927748123075 2211 10-11 0.012170380729095598 2211 12-13 -0.03069365988142181 2211 14-15 -0.3505693174699971 2211 16-17 -0.2391744934269937 2211 18-19 -0.12523081102941092 2211 20-21 -0.14495768902150985 2211 22-23 -0.27819204296183386 2211 24-25 -0.20397640819135887 2211 26-27 -0.3510102467224101 2211 28-29 -0.12877584760958172 2211 30-31 0.03403513572528283 2211 32-33 0.025631953322964307 2211 34-35 0.0691413161652008 2211 36-37 -0.2550881173385591 2211 38-39 -0.28270957083661585 2211 40-41 -0.10800763962192406 2211 42-43 0.10242748810285462 2211 44-45 0.03351449088690117 2211 46-47 0.09836017189461188 2211 48-49 -0.1222188583464785 2211 50-51 -0.14698836475412946 2211 52-53 -0.2553554607212618 2211 54-55 -0.33453710953356364 2211 56-57 -0.38523023066675677 2211 58-59 -0.19380295987780016 2211 60-61 0.06975032491892108 2211 62-63 8.174762922337209E-4 2211 64-65 -0.14085957662995696 2211 66-67 0.10760400063729492 2211 68-69 -0.22214927992013855 2211 70-71 -0.33007809658308673 2211 72-73 -0.23454796446605286 2211 74-75 0.07004786369086347 2211 76-77 0.05666645341375087 2211 78-79 0.34332479762998247 2211 80-81 -0.05550726662555405 2211 82-83 -0.6603203800949125 2211 84-85 -0.24085048587210167 2211 86-87 -0.18695550411582218 2211 88-89 -0.06067476748519596 2211 90-91 -0.07476327472789634 2211 92-93 -0.227638853840336 2211 94-95 0.032026056319036655 2211 96-97 0.04272320651201156 2211 98-99 -0.3367112021628138 2211 100 -0.5588873817966515 2212 1 0.7392721694658242 2212 2 3.9538825303183067 2212 3 2.3078971459909923 2212 4 1.1934571365562476 2212 5 0.6013472593894562 2212 6 0.2887800083551184 2212 7 0.21479636113533473 2212 8 0.2916914065715872 2212 9 0.11286454320448058 2212 10-11 0.2780712199648576 2212 12-13 0.26857721685210834 2212 14-15 0.3254112789230703 2212 16-17 0.2349907348332323 2212 18-19 0.2752632071837837 2212 20-21 0.20644103724478668 2212 22-23 0.189806885673363 2212 24-25 -0.24059922826159408 2212 26-27 -0.04889430124298144 2212 28-29 -0.2957093634717296 2212 30-31 -0.07078600587343686 2212 32-33 0.22662594177603523 2212 34-35 0.3920774509190892 2212 36-37 0.04351315639514297 2212 38-39 0.40893080771228085 2212 40-41 0.5093097819601198 2212 42-43 0.1331905379213154 2212 44-45 0.5336513875001998 2212 46-47 0.4186774149158552 2212 48-49 0.16122260347126627 2212 50-51 0.00904699028426137 2212 52-53 0.03699618501411095 2212 54-55 0.15627296129555646 2212 56-57 0.63810766588702 2212 58-59 0.055283404029111693 2212 60-61 0.3092539258950495 2212 62-63 0.18350898275017613 2212 64-65 0.21719053943456856 2212 66-67 0.23276916937725645 2212 68-69 0.4191280250021876 2212 70-71 0.08455496097300852 2212 72-73 0.43115348187642866 2212 74-75 0.33105018498125816 2212 76-77 0.21790983178408752 2212 78-79 -0.1370007782285967 2212 80-81 0.14874545642664572 2212 82-83 0.33594174715941705 2212 84-85 0.5400018443463388 2212 86-87 0.351878493860486 2212 88-89 0.6663831457342546 2212 90-91 0.48970015006824497 2212 92-93 0.28128323413061906 2212 94-95 0.3581375972987395 2212 96-97 0.5135791079464518 2212 98-99 0.06106869189111208 2212 100 0.5048945359463382 2213 1 -0.6478424186611882 2213 2 -25.951777847040184 2213 3 -14.864629315122675 2213 4 -6.0735930245114 2213 5 -1.97292793619269 2213 6 -1.1223106497212711 2213 7 -0.735594803153667 2213 8 -0.7965276454302526 2213 9 -0.8643558893772081 2213 10-11 -0.7033139618114888 2213 12-13 -1.0027987610586209 2213 14-15 -1.1984957620341277 2213 16-17 -1.4924367847249798 2213 18-19 -1.5644744826459487 2213 20-21 -1.5160163948767007 2213 22-23 -1.5069717613583862 2213 24-25 -1.3171063612574514 2213 26-27 -1.3769200720667243 2213 28-29 -1.2355851112858076 2213 30-31 -1.2612830146171135 2213 32-33 -1.2387675234793747 2213 34-35 -1.2310702711241746 2213 36-37 -1.213297704166294 2213 38-39 -1.0861911435072358 2213 40-41 -0.858384649701172 2213 42-43 -0.986873889138046 2213 44-45 -0.8912450690115392 2213 46-47 -0.873683376616583 2213 48-49 -0.9244193661559805 2213 50-51 -1.0338430235215412 2213 52-53 -1.259483336384875 2213 54-55 -1.1666906834352346 2213 56-57 -1.2162181509560526 2213 58-59 -1.2160465545534933 2213 60-61 -0.9827387110124519 2213 62-63 -0.7045029822751303 2213 64-65 -0.7931177900638318 2213 66-67 -1.3227991693250019 2213 68-69 -0.5154785097424082 2213 70-71 -0.7670096961829955 2213 72-73 -0.8146587592648515 2213 74-75 -1.2995066488889648 2213 76-77 -1.3752562979904184 2213 78-79 -1.233135154666698 2213 80-81 -1.010458409196918 2213 82-83 -0.9733541571203794 2213 84-85 -1.0041997203108153 2213 86-87 -1.2023782939904102 2213 88-89 -1.3343071441875125 2213 90-91 -0.5607600432129338 2213 92-93 -1.023341708345221 2213 94-95 -1.0242600096961958 2213 96-97 -1.4604659909951074 2213 98-99 -0.9975829417950379 2213 100 -1.1640424175741373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 5.0 5 9.0 6 6.0 7 7.0 8 9.0 9 11.0 10 80.0 11 320.0 12 824.0 13 1480.0 14 2239.0 15 2915.0 16 3439.0 17 3712.0 18 3876.0 19 4303.0 20 5300.0 21 6739.0 22 8059.0 23 7956.0 24 6599.0 25 5775.0 26 5654.0 27 6163.0 28 7269.0 29 8802.0 30 10528.0 31 13193.0 32 17373.0 33 23108.0 34 32718.0 35 47024.0 36 73347.0 37 114807.0 38 81219.0 39 680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.683204220862024 20.479668770721755 10.6812675337924 27.155859474623824 2 12.01879970474288 23.896643326897173 33.82654061245 30.258016355909938 3 18.783144660557134 29.860409178932212 27.858427175209528 23.49801898530113 4 11.833987106524479 21.142688718901265 31.65627157402077 35.367052600553485 5 11.018255449491408 38.476519933587724 34.498718116364124 16.00650650055674 6 32.406817363219325 32.819840965133444 17.676301905014466 17.09703976663276 7 22.242052735093807 34.621305357800054 23.67004752727575 19.46659437983039 8 27.43799036340071 33.25576563473925 21.306976145953914 17.999267855906126 9 26.89379923343748 14.87495374703437 22.278000605493368 35.95324641403479 10-11 22.450716070147667 28.04472037398897 26.057038264612036 23.447525291251328 12-13 26.687806398314095 26.006025318209026 27.508892043908638 19.797276239568234 14-15 26.65763676443234 24.335421621899723 26.17748210179714 22.82945951187079 16-17 20.261774148071567 28.546836895710804 31.38034913112946 19.811039825088173 18-19 22.435268989955848 26.45767814197541 33.301214735906974 17.805838132161764 20-21 21.25775188622224 26.176125229131365 32.630878153060245 19.93524473158615 22-23 19.284724344953865 26.569444732971075 30.103088092291593 24.042742829783467 24-25 20.66392594512358 27.355117980940246 28.83999422311821 23.140961850817963 26-27 22.402464126640602 24.45471603492429 30.634122891787925 22.508696946647188 28-29 20.99599666438159 23.53282745099203 33.71229994450528 21.7588759401211 30-31 23.05803584677571 26.187258744551656 30.640307540722834 20.1143978679498 32-33 18.11699126877688 26.277737556919107 32.32026221362419 23.285008960679825 34-35 17.35940086997288 26.969554905416658 33.35163085298091 22.319413371629548 36-37 23.13687556685726 25.929003013677764 30.48186205129879 20.452259368166185 38-39 23.272274094002217 24.68418658015509 30.38534578256053 21.658193543282163 40-41 20.49099985262685 26.167042023106923 30.61108424106291 22.730873883203316 42-43 23.25345165409377 25.294722151612653 30.576427964555204 20.87539822973838 44-45 25.12769181466784 23.558299077770346 28.969227360666572 22.34478174689524 46-47 21.69158559565555 25.899727482701827 32.92404631310309 19.484640608539536 48-49 23.83539527807071 25.97456813914516 30.714304086231696 19.475732496552432 50-51 21.175274212184043 26.770805305392443 29.82344375682563 22.230476725597885 52-53 19.716118131104434 27.076410481152855 27.238453449249782 25.96901793849292 54-55 21.465208784881977 27.919827713273577 25.661977320593188 24.952986181251255 56-57 23.203148724614202 24.929942064337567 27.227090585182832 24.639818625865402 58-59 21.675364601055623 23.697584888938128 30.6369736727572 23.990076837249052 60-61 23.237549617059663 25.92975421194805 28.347278368890784 22.485417802101498 62-63 18.82716843169413 26.521705584318724 29.409815438731158 25.241310545255992 64-65 17.919881930850018 26.88654935589203 30.584618367389332 24.608950345868617 66-67 22.9183009089767 25.820616331508916 28.231595670069847 23.029487089444533 68-69 23.429776061745507 24.627272448481936 28.00014641013237 23.942805079640188 70-71 20.996363478081943 25.88162903519394 28.28483977935532 24.837167707368803 72-73 23.243020802317147 25.280320349155254 28.73833832724798 22.738320521279615 74-75 24.932435908089094 23.73616568337666 27.11486489160069 24.216533516933563 76-77 21.454219208109613 25.747021607441727 31.13720982681602 21.661549357632637 78-79 23.26848187446401 25.79732278775877 30.105234837192143 20.82896050058508 80-81 20.733797763967292 26.32120887805679 29.62827415189315 23.31671920608277 82-83 19.155716666584244 26.505412770082142 27.62179350863224 26.71707705470137 84-85 20.779278584230454 26.666547973713467 26.26348648680728 26.2906869552488 86-87 22.30449296506021 24.717687495363617 27.547488934497167 25.430330605079003 88-89 21.132080697024154 23.59933969047744 30.319146305639432 24.94943330685897 90-91 22.323680509260814 25.392716763234468 28.42921207397575 23.854390653528963 92-93 18.59646728245421 26.126561846294443 29.208268920927775 26.068701950323568 94-95 17.760676186501193 26.358000636941416 30.053942212402156 25.827380964155232 96-97 21.959151055641875 25.578212050151766 28.075316662891865 24.38732023131449 98-99 22.15461668252408 24.558646377092536 27.85566920583052 25.43106773455287 100 19.719827159418106 25.779743115487136 29.842724575918762 24.657705149176 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 48.0 1 35.5 2 22.5 3 25.5 4 40.5 5 131.5 6 445.0 7 958.5 8 1449.0 9 1489.0 10 903.5 11 400.0 12 294.0 13 284.0 14 288.0 15 311.0 16 345.0 17 385.5 18 467.5 19 519.5 20 579.5 21 708.0 22 877.0 23 1106.0 24 1393.0 25 1768.5 26 2284.0 27 2993.5 28 3800.5 29 4675.5 30 5716.0 31 6733.0 32 7641.5 33 8561.0 34 9363.0 35 10039.0 36 10842.0 37 11708.5 38 12444.5 39 13131.0 40 13301.0 41 13318.0 42 13314.5 43 13155.5 44 13077.0 45 12984.0 46 13869.5 47 16409.5 48 19215.0 49 27782.5 50 60124.5 51 61076.5 52 25551.0 53 12126.5 54 9607.0 55 8689.0 56 7882.5 57 6730.0 58 5702.0 59 4959.5 60 4252.5 61 3571.0 62 3025.5 63 2539.5 64 2055.5 65 1605.0 66 1239.5 67 872.5 68 607.5 69 490.5 70 400.5 71 271.0 72 172.5 73 138.0 74 104.5 75 64.0 76 35.5 77 19.5 78 15.0 79 7.0 80 0.5 81 1.0 82 1.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7241632364024061 2 5.136396274149489 3 0.0 4 0.004351704780347701 5 0.16279331973755262 6 0.04964899544851241 7 0.030659738225176987 8 0.03639607634472623 9 0.03481363824278161 10-11 0.03689058825158392 12-13 0.03590156443786854 14-15 0.03778070968392777 16-17 0.029967421555576215 18-19 0.02581352153797159 20-21 0.021264011994880812 22-23 0.021264011994880812 24-25 0.017011209595904648 26-27 0.010978164332240792 28-29 0.018890354841963886 30-31 0.02373657152916928 32-33 0.0013846333392015413 34-35 0.0031648762038892376 36-37 0.004054997636233085 38-39 0.005736338119549243 40-41 0.005835240500920781 42-43 0.006033045263663859 44-45 0.006923166696007707 46-47 0.015132064349845415 48-49 0.02373657152916928 50-51 0.01997828103705081 52-53 0.02522010724974236 54-55 0.0222530358085962 56-57 0.014736454824359263 58-59 0.012857309578300025 60-61 0.01038475004401156 62-63 0.015824381019446186 64-65 0.01631889292630388 66-67 0.017901331028248497 68-69 0.023637669147797743 70-71 0.023934376291912356 72-73 0.01987937865567927 74-75 0.020373890562536964 76-77 0.014835357205730799 78-79 0.011967188145956178 80-81 0.010978164332240792 82-83 0.006230850026406936 84-85 0.008505604797952324 86-87 0.007219873840122322 88-89 0.0056374357381777035 90-91 0.006329752407778475 92-93 0.0034615833480038534 94-95 0.0015824381019446188 96-97 6.923166696007707E-4 98-99 3.956095254861547E-4 100 0.11373773857726946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 505549.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.11454050289055 #Duplication Level Percentage of deduplicated Percentage of total 1 82.18656718502365 42.8311518435745 2 11.291108375864509 11.768618495530351 3 3.459154339472545 5.408167167905746 4 1.3015561146930203 2.7131999542381706 5 0.6197372869453814 1.6148661970833291 6 0.30072132423225684 0.9403172179070898 7 0.18408211238615774 0.6715348291264247 8 0.10856311163226384 0.4526173342623554 9 0.07173951646344706 0.33648047427528854 >10 0.38002938194020547 3.715744622446903 >50 0.0457045281987402 1.6557969661128584 >100 0.03313578293925499 3.2202888965737064 >500 0.00571306602400948 1.9573020715676355 >1k 0.009902647774949766 11.597327394539478 >5k 0.001523484273069195 4.57636715972654 >10k+ 7.617421365345975E-4 6.54021937512966 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 18935 3.745433182540169 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 14015 2.772233749844229 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6338 1.2536865862656241 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 5939 1.1747624859311363 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 5763 1.1399488476883546 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 5016 0.9921886899192758 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 4740 0.9375945754021866 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 4191 0.828999760656237 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3927 0.7767793032920647 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3219 0.636733531269966 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2986 0.5906450215508289 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2984 0.5902494120253428 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2544 0.5032153164183887 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 2387 0.4721599686677256 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 2333 0.46147851147959945 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 2102 0.41578561128594854 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 2098 0.41499439223497625 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 2066 0.4086646398271977 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 2056 0.40668659219976705 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 1954 0.3865105063999731 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 1923 0.3803785587549377 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1898 0.3754334396863608 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 1818 0.35960905866691456 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 1767 0.3495210157670176 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1746 0.34536711574941303 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1740 0.34418028717295457 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 1590 0.31450957276149294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.2955203155381575 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 1321 0.2613000915836051 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 1303 0.2577396058542298 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 1123 0.22213474856047585 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 1118 0.22114572474676045 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 914 0.1807935531471727 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 834 0.1649691721277265 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 804 0.15903502924543417 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 756 0.14954040063376647 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 688 0.13608967676723718 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 656 0.1297599243594587 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 645 0.12758407196928487 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 622 0.12303456242619411 No Hit ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT 621 0.12283675766345103 No Hit CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 574 0.11353993381452637 No Hit CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC 574 0.11353993381452637 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 573 0.1133421290517833 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 550 0.10879261950869253 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.10701237664400484 No Hit GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 509 0.10068262423622637 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.9780476274307732E-4 0.0 0.0 0.0 0.0 9 1.9780476274307732E-4 0.0 0.0 0.0 0.0 10-11 1.9780476274307732E-4 0.0 0.0 0.0 0.0 12-13 1.9780476274307732E-4 0.0 0.0 1.9780476274307732E-4 0.0 14-15 1.9780476274307732E-4 0.0 0.0 1.9780476274307732E-4 0.0 16-17 1.9780476274307732E-4 0.0 0.0 1.9780476274307732E-4 0.0 18-19 1.9780476274307732E-4 0.0 0.0 1.9780476274307732E-4 0.0 20-21 1.9780476274307732E-4 0.0 0.0 3.9560952548615464E-4 0.0 22-23 1.9780476274307732E-4 0.0 0.0 0.00148353572057308 0.0 24-25 1.9780476274307732E-4 0.0 0.0 0.002670364297031544 0.0 26-27 1.9780476274307732E-4 0.0 0.0 0.004549509543090779 0.0 28-29 1.9780476274307732E-4 0.0 0.0 0.008802311942066942 0.0 30-31 1.9780476274307732E-4 0.0 0.0 0.023044254859568507 0.0 32-33 1.9780476274307732E-4 0.0 0.0 0.05815460024646474 0.0 34-35 1.9780476274307732E-4 0.0 0.0 0.11195749571258178 0.0 36-37 2.9670714411461597E-4 0.0 0.0 0.18910135318238191 0.0 38-39 3.9560952548615464E-4 0.0 0.0 0.3048171393870822 0.0 40-41 3.9560952548615464E-4 0.0 0.0 0.49352288304397796 0.0 42-43 3.9560952548615464E-4 0.0 0.0 0.6277334145651559 0.0 44-45 3.9560952548615464E-4 0.0 0.0 0.7704495508842861 0.0 46-47 3.9560952548615464E-4 0.0 0.0 0.9454078635305381 0.0 48-49 3.9560952548615464E-4 0.0 0.0 1.1055308189710593 0.0 50-51 3.9560952548615464E-4 0.0 0.0 1.270203283954671 0.0 52-53 3.9560952548615464E-4 0.0 0.0 1.481755477708392 0.0 54-55 3.9560952548615464E-4 0.0 0.0 1.6907362095464533 0.0 56-57 3.9560952548615464E-4 0.0 0.0 1.92384912243917 0.0 58-59 3.9560952548615464E-4 0.0 0.0 2.1903910402354665 0.0 60-61 3.9560952548615464E-4 0.0 0.0 2.4413063817750604 0.0 62-63 3.9560952548615464E-4 0.0 0.0 2.704485618604725 0.0 64-65 3.9560952548615464E-4 0.0 0.0 3.028984331884743 0.0 66-67 3.9560952548615464E-4 0.0 0.0 3.3659447452175755 0.0 68-69 3.9560952548615464E-4 0.0 0.0 3.776587432672204 0.0 70-71 3.9560952548615464E-4 0.0 0.0 4.41658474252743 0.0 72-73 3.9560952548615464E-4 0.0 0.0 4.9077339684184915 0.0 74-75 3.9560952548615464E-4 0.0 0.0 5.365454189405972 0.0 76-77 3.9560952548615464E-4 0.0 0.0 5.915252527450356 0.0 78-79 3.9560952548615464E-4 0.0 0.0 6.444775877313574 0.0 80-81 3.9560952548615464E-4 0.0 0.0 7.044717722713328 0.0 82-83 3.9560952548615464E-4 0.0 0.0 7.814573859309384 0.0 84-85 3.9560952548615464E-4 0.0 0.0 8.534484293312815 0.0 86-87 3.9560952548615464E-4 0.0 0.0 9.244900098704576 0.0 88 3.9560952548615464E-4 0.0 0.0 9.867094979912926 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAGCA 45 0.0010169832 41.74795 9 CTAGAGT 105 2.0563002E-6 31.345093 4 AGTACTC 290 0.0 29.183365 5 GTAGCAC 65 0.0061396766 28.933933 3 TAGATGC 65 0.0061396766 28.933933 4 GTTGACC 65 0.006172447 28.902424 8 GCCTAAC 90 6.9450785E-4 27.554476 1 GTCTAGA 130 2.1930027E-4 22.89141 1 AAGTACT 360 0.0 20.896729 4 TAGACCA 115 0.0039470196 20.442453 4 ATAGACT 115 0.0039470196 20.442453 4 ACTGTAC 140 5.19181E-4 20.150415 3 ATGTACC 730 0.0 19.950579 94 AAAGTAC 380 0.0 19.796902 3 CTTAGAG 120 0.005049304 19.590683 3 AATGCCG 60 0.005105081 19.5558 30-31 TCTATAC 145 6.576236E-4 19.455576 3 TATAAGG 130 0.0059205163 19.058245 2 GAGTACT 3845 0.0 18.810076 12-13 ATAGTAC 125 0.0063924375 18.807056 3 >>END_MODULE