FastQCFastQC Report
Wed 25 May 2016
SRR1295141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672046
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27210.40488299908042014No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21810.32453135648452636No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9830.1462697493921547No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9750.14507935468703034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9380.13957377917583025No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9260.1377881871181437No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7670.11412909235379722No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7180.10683792478491055No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG1150.00140426224.422924
GTTCTAA1300.001604956123.8684431
ACACTCG1302.0201906E-423.1715346
TATACCT2603.110472E-1023.1715345
GTACAAA2202.8930644E-622.5665281
GGTAATA2202.898605E-622.5615562
GTATAGT1702.9561465E-421.9028051
ATACCTT2305.0118615E-821.8282576
TAACGCC1050.008125151521.399134
GTATAGG2156.184733E-520.2049121
GTACATA2207.380732E-519.7457121
ATATACT1757.0407946E-419.2592164
AGTACTC3153.4124241E-919.1257115
CATATAC1808.515086E-418.7223723
ATGGGTA3809.458745E-1118.4965745
ATGTAGT1750.0089779317.7308431
CATGGGT6400.017.5539744
TTAATGT2602.895743E-517.2839134
GTAATAA2954.460324E-617.1357293
CTATACC2302.2270532E-417.0960454
Produced by FastQC (version 0.11.5)