##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295141_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 672046 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.05569856825277 27.0 27.0 33.0 6.0 33.0 2 22.201051713721977 27.0 6.0 33.0 2.0 33.0 3 24.371296012475337 27.0 15.0 33.0 6.0 33.0 4 24.330754144805564 33.0 6.0 37.0 2.0 37.0 5 26.40640670430298 33.0 15.0 37.0 2.0 37.0 6 27.248743389589404 33.0 15.0 37.0 2.0 37.0 7 26.919742100987136 33.0 15.0 37.0 6.0 37.0 8 27.687204447314617 33.0 15.0 37.0 6.0 37.0 9 28.5577847349735 33.0 22.0 37.0 6.0 37.0 10-11 28.15435327343664 33.0 18.5 37.0 6.0 37.0 12-13 26.936057204417555 33.0 15.0 37.0 4.0 37.0 14-15 28.688280266529375 33.0 18.5 37.0 6.0 40.0 16-17 29.095303000092255 33.0 22.0 37.0 6.0 40.0 18-19 29.26762825758951 33.0 22.0 37.0 6.0 40.0 20-21 28.73166940953447 33.0 22.0 37.0 6.0 40.0 22-23 29.534885260830357 33.0 24.5 37.0 6.0 40.0 24-25 29.681480880773044 33.0 24.5 37.0 6.0 40.0 26-27 28.270250845924238 33.0 18.5 37.0 4.0 40.0 28-29 28.961254884338274 33.0 22.0 37.0 6.0 40.0 30-31 29.662221782437513 33.0 27.0 37.0 10.5 40.0 32-33 28.34457760331882 33.0 22.0 37.0 6.0 40.0 34-35 27.9764249768617 33.0 22.0 37.0 6.0 40.0 36-37 28.254832258506113 33.0 22.0 37.0 6.0 40.0 38-39 28.371497635578514 33.0 22.0 37.0 6.0 40.0 40-41 27.36352645503433 33.0 15.0 37.0 6.0 40.0 42-43 27.909229427747505 33.0 22.0 37.0 6.0 40.0 44-45 27.762721599414327 33.0 22.0 37.0 6.0 40.0 46-47 27.373508658633483 33.0 22.0 37.0 6.0 40.0 48-49 27.226323793311767 33.0 18.5 37.0 6.0 40.0 50-51 27.130263404588376 33.0 22.0 37.0 6.0 40.0 52-53 27.32448225270294 33.0 22.0 37.0 6.0 40.0 54-55 27.234886451225066 33.0 22.0 37.0 6.0 40.0 56-57 27.173450180493596 33.0 22.0 37.0 6.0 40.0 58-59 27.02798245953402 33.0 22.0 37.0 6.0 40.0 60-61 26.93929359002211 33.0 22.0 37.0 6.0 37.0 62-63 26.817850266202015 27.0 22.0 37.0 6.0 37.0 64-65 26.35221026536874 27.0 18.5 37.0 6.0 37.0 66-67 26.527858956083364 27.0 22.0 37.0 6.0 37.0 68-69 26.036559997381133 27.0 18.5 35.0 6.0 37.0 70-71 26.120534159864057 27.0 18.5 33.0 6.0 37.0 72-73 26.101625483969848 27.0 22.0 33.0 6.0 37.0 74-75 26.22124601589772 27.0 22.0 33.0 6.0 37.0 76-77 25.946551277739914 27.0 22.0 33.0 6.0 37.0 78-79 25.539411736696593 27.0 22.0 33.0 6.0 37.0 80-81 25.40860967850415 27.0 22.0 33.0 6.0 37.0 82-83 25.53179395458049 27.0 22.0 33.0 6.0 37.0 84-85 25.451347080408187 27.0 22.0 33.0 6.0 37.0 86-87 25.21262309425248 27.0 22.0 33.0 6.0 37.0 88-89 25.15308103909554 27.0 22.0 33.0 6.0 37.0 90-91 25.310181892310943 27.0 22.0 33.0 6.0 37.0 92-93 25.231996768078375 27.0 22.0 33.0 6.0 37.0 94-95 25.095229344419877 27.0 18.5 33.0 6.0 37.0 96-97 25.215306690315842 27.0 22.0 33.0 6.0 37.0 98-99 25.273232040663885 27.0 22.0 33.0 6.0 37.0 100 24.90490234299438 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.059152100169683 1101 2 11.05935844769634 1101 3 8.22729953195023 1101 4 12.537026053192303 1101 5 10.42476976333786 1101 6 9.654373470053383 1101 7 9.238061245243092 1101 8 8.853055665592354 1101 9 7.2333016119468745 1101 10-11 7.782384622136835 1101 12-13 9.156397103276795 1101 14-15 7.901713211531295 1101 16-17 8.153223354944338 1101 18-19 7.875643659506082 1101 20-21 7.159956900013267 1101 22-23 7.2825147017426595 1101 24-25 6.8742165927525 1101 26-27 8.335577850764277 1101 28-29 7.986807292004624 1101 30-31 5.266642660764251 1101 32-33 6.657285468344252 1101 34-35 8.636977159186273 1101 36-37 7.914080316594845 1101 38-39 7.96255008975152 1101 40-41 8.86489875467289 1101 42-43 8.308753409834754 1101 44-45 8.174748833331332 1101 46-47 7.1358638259811435 1101 48-49 5.268265389860407 1101 50-51 6.502243579641956 1101 52-53 5.65193171173118 1101 54-55 3.859201715386437 1101 56-57 5.946192216544837 1101 58-59 5.927825536675144 1101 60-61 6.3050629558421 1101 62-63 5.91605407128462 1101 64-65 6.2530442651369995 1101 66-67 5.482264645902383 1101 68-69 6.429425335345449 1101 70-71 5.907343926212683 1101 72-73 5.048852821226252 1101 74-75 4.529256539565814 1101 76-77 4.754875469978973 1101 78-79 4.982554632085076 1101 80-81 4.650024812941801 1101 82-83 4.023467565833592 1101 84-85 3.981816789580982 1101 86-87 3.569847042085616 1101 88-89 3.6947799039253297 1101 90-91 3.304397805581356 1101 92-93 3.2687238832874463 1101 94-95 3.3789342933177835 1101 96-97 3.4502460367696166 1101 98-99 3.2774103497964298 1101 100 3.652919714435253 1102 1 6.829650110694647 1102 2 10.447370256193324 1102 3 8.09036589056915 1102 4 12.525223973870009 1102 5 10.580423723034944 1102 6 9.255406713539461 1102 7 6.089569395673077 1102 8 7.7498981761814925 1102 9 6.204749436359663 1102 10-11 7.088632215513808 1102 12-13 8.781210991096067 1102 14-15 5.894044106153295 1102 16-17 6.974642299880806 1102 18-19 7.1863354834054505 1102 20-21 6.898645771788392 1102 22-23 6.387815690062514 1102 24-25 6.7116957353529365 1102 26-27 8.139553592004173 1102 28-29 7.429449443204728 1102 30-31 3.7645858002842125 1102 32-33 4.365910805271476 1102 34-35 5.190709029292801 1102 36-37 5.159629668411046 1102 38-39 4.598450487646534 1102 40-41 6.392943935009175 1102 42-43 7.079033219871974 1102 44-45 6.448747678152277 1102 46-47 5.465818260585177 1102 48-49 3.369167071287695 1102 50-51 4.625080827748743 1102 52-53 5.37792965807953 1102 54-55 5.110985825256801 1102 56-57 6.3145691899757175 1102 58-59 5.876805128511876 1102 60-61 5.5856495300944395 1102 62-63 3.988945869513344 1102 64-65 5.804876507467892 1102 66-67 3.5330604359165 1102 68-69 5.740595275019437 1102 70-71 5.120326855233344 1102 72-73 4.950232618428153 1102 74-75 4.005617083783502 1102 76-77 2.9801803088956724 1102 78-79 4.180809028183138 1102 80-81 3.9594555663752473 1102 82-83 3.6192447446296363 1102 84-85 2.6056904900045517 1102 86-87 2.717947414309979 1102 88-89 3.021920194003627 1102 90-91 2.8917036713238815 1102 92-93 2.958725166819729 1102 94-95 2.808388150332288 1102 96-97 2.9846928343565757 1102 98-99 2.9386284219309466 1102 100 2.5030929912931654 1103 1 6.447701733257549 1103 2 10.73677884259439 1103 3 7.750923127818115 1103 4 11.384241647925279 1103 5 10.004548971220341 1103 6 8.84048304072105 1103 7 7.775365053649185 1103 8 7.579328895837779 1103 9 6.940781073895057 1103 10-11 7.080249245100756 1103 12-13 8.497794658055682 1103 14-15 8.117769002810519 1103 16-17 7.247193808522546 1103 18-19 6.673200372901917 1103 20-21 7.592328029380422 1103 22-23 6.38072979350294 1103 24-25 4.499569640896016 1103 26-27 5.159922696583802 1103 28-29 6.373872188379355 1103 30-31 5.6213689920298116 1103 32-33 7.515979157971408 1103 34-35 6.734707011260706 1103 36-37 3.6507080136247296 1103 38-39 5.981798467986994 1103 40-41 6.21612201006695 1103 42-43 7.11495093624082 1103 44-45 4.999538616537205 1103 46-47 5.783539511765074 1103 48-49 5.035345504383422 1103 50-51 5.8334849549498635 1103 52-53 5.607895807218615 1103 54-55 4.368042427802127 1103 56-57 5.2671226515517375 1103 58-59 4.735925331617043 1103 60-61 4.545113124144503 1103 62-63 2.311800082969061 1103 64-65 4.656523482047199 1103 66-67 3.6323020464363047 1103 68-69 4.739151731094687 1103 70-71 3.2529538773340505 1103 72-73 3.8422858033384806 1103 74-75 2.5958149811242563 1103 76-77 2.849405319474066 1103 78-79 2.401318093053103 1103 80-81 3.676200350413268 1103 82-83 3.1685042570470436 1103 84-85 2.9234719566581937 1103 86-87 2.430962093866011 1103 88-89 2.6275169781718404 1103 90-91 2.2695443769841326 1103 92-93 1.8449049018716401 1103 94-95 2.448787850882365 1103 96-97 2.407324087814896 1103 98-99 2.045855906226098 1103 100 2.250754352488361 1104 1 6.4857123396450085 1104 2 10.022657193921528 1104 3 7.443480432397077 1104 4 12.266547291160538 1104 5 9.812384610959047 1104 6 8.326748644293566 1104 7 4.459666461791336 1104 8 6.519341628859976 1104 9 6.804508080195081 1104 10-11 7.0942969446664925 1104 12-13 7.64933436360813 1104 14-15 7.256004565634498 1104 16-17 7.550115372244811 1104 18-19 7.034592672915267 1104 20-21 7.588952965344614 1104 22-23 6.521072379722288 1104 24-25 6.176560323595513 1104 26-27 8.42729726890111 1104 28-29 7.441226862359937 1104 30-31 6.500757567801582 1104 32-33 7.475732858655455 1104 34-35 8.2685487726079 1104 36-37 6.847308602825958 1104 38-39 7.514561844715605 1104 40-41 7.32223419555006 1104 42-43 7.0404900209268995 1104 44-45 6.557024480825763 1104 46-47 6.287759059297944 1104 48-49 5.286829867686873 1104 50-51 5.228320332087922 1104 52-53 5.521192631568436 1104 54-55 4.440289653156054 1104 56-57 5.993766859246364 1104 58-59 3.8228820197302262 1104 60-61 4.211112367433717 1104 62-63 3.713554915371013 1104 64-65 2.8472978286905715 1104 66-67 3.1001212700472145 1104 68-69 4.127120507925753 1104 70-71 2.328051204595205 1104 72-73 1.7661904501262633 1104 74-75 2.7290341062957957 1104 76-77 3.5988670838155805 1104 78-79 3.9893459909038462 1104 80-81 2.7339234641011707 1104 82-83 2.689546987290175 1104 84-85 2.926292880715909 1104 86-87 0.5486241636661262 1104 88-89 0.08260317958414021 1104 90-91 1.906701076864124 1104 92-93 2.466494740709468 1104 94-95 2.5276319010166084 1104 96-97 3.0501922321562844 1104 98-99 2.5756699285265867 1104 100 0.7750085594798541 1105 1 5.735192533283971 1105 2 -2.5753995096979523 1105 3 0.5320918887134383 1105 4 8.293673149290694 1105 5 8.725380897131451 1105 6 8.259776960610186 1105 7 5.205745790837039 1105 8 6.064010442061118 1105 9 5.126707445060056 1105 10-11 4.9662972362856515 1105 12-13 5.0586986002589995 1105 14-15 5.884292295902121 1105 16-17 5.919924922868315 1105 18-19 6.032768134601639 1105 20-21 6.967276788279978 1105 22-23 1.7803023900200863 1105 24-25 4.033712259577431 1105 26-27 6.996823737896662 1105 28-29 6.005146617207981 1105 30-31 5.434830772844858 1105 32-33 7.2864091896835035 1105 34-35 6.343069699400459 1105 36-37 6.2757487136988 1105 38-39 4.049416809747683 1105 40-41 5.408307174204783 1105 42-43 5.551962213265082 1105 44-45 6.160035112934278 1105 46-47 5.291686085439586 1105 48-49 4.705778206488862 1105 50-51 5.745549604704355 1105 52-53 5.389321534912511 1105 54-55 5.047274075992323 1105 56-57 5.247296386406038 1105 58-59 2.029338314507317 1105 60-61 4.334954104563021 1105 62-63 -0.35099412293325827 1105 64-65 -0.32637241430781216 1105 66-67 0.9171880417223583 1105 68-69 2.785711210358617 1105 70-71 2.6871403935342038 1105 72-73 2.60296070075087 1105 74-75 2.4192129676476704 1105 76-77 1.7433682159981991 1105 78-79 2.984113873082027 1105 80-81 1.3957694613643596 1105 82-83 0.8809438862465306 1105 84-85 1.2587363279421915 1105 86-87 -0.6454085792183477 1105 88-89 1.3436052079680891 1105 90-91 1.247288756625995 1105 92-93 1.684984882494895 1105 94-95 0.9553530363552376 1105 96-97 1.783420413119405 1105 98-99 1.8204077134967847 1105 100 1.9585041619300299 1106 1 6.075158451017078 1106 2 10.418216402871174 1106 3 6.857973755632983 1106 4 11.695813853657544 1106 5 9.779734132130091 1106 6 9.305080555679051 1106 7 6.61522404109289 1106 8 7.439680116354825 1106 9 6.887835821598689 1106 10-11 5.502463638493957 1106 12-13 7.723492698299502 1106 14-15 7.528397847649892 1106 16-17 7.652081494736834 1106 18-19 7.121544224436107 1106 20-21 8.147576439946068 1106 22-23 6.0249813782556 1106 24-25 4.847419339863233 1106 26-27 7.354719932512982 1106 28-29 6.200428382725743 1106 30-31 5.100568001383831 1106 32-33 7.184501803314532 1106 34-35 6.678594594477779 1106 36-37 5.970879784895995 1106 38-39 6.8656110505018155 1106 40-41 6.3967203770928585 1106 42-43 6.081868463558489 1106 44-45 4.556729226936177 1106 46-47 5.762761695493385 1106 48-49 5.563168096355255 1106 50-51 6.597287518644283 1106 52-53 5.526697247307002 1106 54-55 4.912819298372074 1106 56-57 4.414848753856063 1106 58-59 4.477923384033261 1106 60-61 4.533492228817767 1106 62-63 4.715986685839873 1106 64-65 4.762664691234548 1106 66-67 2.8047243069443617 1106 68-69 3.1757597517690392 1106 70-71 2.4204083946984483 1106 72-73 2.321048479018966 1106 74-75 2.36482405424292 1106 76-77 3.255548401338128 1106 78-79 3.8056669165439594 1106 80-81 3.3572027474394552 1106 82-83 1.6595741640686477 1106 84-85 2.520039106163342 1106 86-87 3.1443568822067256 1106 88-89 2.977143379168105 1106 90-91 2.860912039602699 1106 92-93 2.67230731200657 1106 94-95 1.0579035729396864 1106 96-97 1.5731072412152116 1106 98-99 1.9568513275315418 1106 100 2.4079154682291453 1107 1 6.305488133926225 1107 2 10.716646211700876 1107 3 6.535495028575326 1107 4 11.748488711519943 1107 5 9.83597864695437 1107 6 8.280463087063797 1107 7 4.027278072458554 1107 8 6.746470286919941 1107 9 5.879708222102373 1107 10-11 4.745567580188922 1107 12-13 6.466229371707371 1107 14-15 6.651656900574686 1107 16-17 7.384536563993137 1107 18-19 6.6716253096423515 1107 20-21 5.922734794584141 1107 22-23 6.117474796044743 1107 24-25 5.0874840172395075 1107 26-27 7.6189308303647 1107 28-29 6.659351571186843 1107 30-31 5.332841821355597 1107 32-33 5.287140411320024 1107 34-35 6.936436010893239 1107 36-37 6.650399569686321 1107 38-39 6.394609982084976 1107 40-41 7.785455257086749 1107 42-43 6.465435831055725 1107 44-45 4.227243784115554 1107 46-47 3.6522578224823583 1107 48-49 3.851215183011494 1107 50-51 5.005048803797106 1107 52-53 3.959950767358894 1107 54-55 5.354719363240239 1107 56-57 5.576050913584382 1107 58-59 3.192996646901964 1107 60-61 3.1226210875196365 1107 62-63 0.40721231258082113 1107 64-65 1.9752529310178133 1107 66-67 3.930093483118668 1107 68-69 5.068211196039655 1107 70-71 4.183561369094598 1107 72-73 2.6082618189663087 1107 74-75 2.4410651348702324 1107 76-77 2.816480595026839 1107 78-79 3.555665962600358 1107 80-81 1.7060551532852202 1107 82-83 1.4708430998491657 1107 84-85 2.411558547533968 1107 86-87 2.319894906398737 1107 88-89 2.460449366118155 1107 90-91 1.379969924900518 1107 92-93 2.022876816337476 1107 94-95 2.254345363301045 1107 96-97 2.2153955611297356 1107 98-99 1.702178911764765 1107 100 0.15123977089637108 1108 1 4.4171532252784225 1108 2 -2.961129149097472 1108 3 0.22701220078639395 1108 4 6.516544510377635 1108 5 6.856501369020776 1108 6 7.161146009949107 1108 7 4.263756195479385 1108 8 5.472626410529831 1108 9 5.16888494543544 1108 10-11 3.363232020881707 1108 12-13 2.628365084246809 1108 14-15 3.892081330718309 1108 16-17 4.72857513770149 1108 18-19 3.8424729764123278 1108 20-21 4.596267875225482 1108 22-23 4.008860873858218 1108 24-25 2.6096292971674337 1108 26-27 4.520430282694271 1108 28-29 6.16628239338549 1108 30-31 4.433299159850566 1108 32-33 4.402799935491636 1108 34-35 2.9934524487061793 1108 36-37 1.0399530209725292 1108 38-39 2.127700777949247 1108 40-41 5.487091338269028 1108 42-43 2.7036847390836662 1108 44-45 3.7007532350398584 1108 46-47 4.292553612638919 1108 48-49 4.652239249709858 1108 50-51 2.1567809438698298 1108 52-53 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-5.461075778540408 2213 50-51 -22.717096042999554 2213 52-53 -17.069916146909133 2213 54-55 -16.961898382189617 2213 56-57 -16.774992656268157 2213 58-59 -17.096496019904478 2213 60-61 -18.462466920786987 2213 62-63 -16.746289299823207 2213 64-65 -16.7954599712096 2213 66-67 -7.4321944832554685 2213 68-69 -21.74341833042274 2213 70-71 -16.567742781223387 2213 72-73 -8.422574784102753 2213 74-75 -3.4706794563379226 2213 76-77 -2.331984126771065 2213 78-79 -3.6604498409559163 2213 80-81 -2.923281614518615 2213 82-83 -2.48820533217528 2213 84-85 -2.206053347315322 2213 86-87 -2.6998420670739733 2213 88-89 -2.3546433346336357 2213 90-91 -2.203219889498296 2213 92-93 -3.3144113996348104 2213 94-95 -3.0887846973013886 2213 96-97 -2.6582191691545027 2213 98-99 -3.0973638244299657 2213 100 -2.341848856413659 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 203.0 3 169.0 4 973.0 5 3287.0 6 5224.0 7 6380.0 8 6309.0 9 6442.0 10 7519.0 11 9291.0 12 11700.0 13 14041.0 14 12171.0 15 13711.0 16 14886.0 17 15543.0 18 15313.0 19 15285.0 20 15094.0 21 15225.0 22 16323.0 23 17774.0 24 18913.0 25 20405.0 26 22003.0 27 23041.0 28 24323.0 29 25083.0 30 27180.0 31 30053.0 32 34885.0 33 40578.0 34 46298.0 35 49814.0 36 47655.0 37 32401.0 38 6549.0 39 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.5265024515979 19.429963112980225 13.021893189144492 25.021641246277383 2 16.517161999928682 21.42466589272908 36.34310528588806 25.715066821454187 3 19.570585726923838 26.551829481737006 27.685987232769648 26.191597558569512 4 14.635257791492261 19.13543751583637 32.91214327663996 33.31716141603141 5 14.47941407648035 37.76514768813991 32.912476218690436 14.842962016689299 6 31.817986337396825 35.347106207717886 17.257389549597235 15.577517905288055 7 27.665447451841015 32.34934568804357 20.826952775745752 19.158254084369666 8 27.1278885003281 33.57443375493179 19.89438492391427 19.40329282082584 9 26.659934678679058 18.180154309787152 19.634894838984522 35.525016172549265 10-11 24.90846069816537 27.817776724190402 25.27822181502494 21.99554076261929 12-13 25.86920106149003 25.883014744858773 25.072084959145613 23.175699234505583 14-15 24.44093888716837 26.7885600547125 23.24458681738412 25.525914240735005 16-17 22.981768037716495 29.235307605117555 25.261674799738465 22.52124955742748 18-19 23.671496812870238 28.470521146232592 26.169046864105518 21.688935176791656 20-21 24.366757005302624 27.54208295083484 25.711609529305672 22.379550514556858 22-23 23.78348201930361 27.406190249510924 24.79381386235069 24.016513868834778 24-25 23.738283285007096 27.975097867524372 24.248684000009458 24.03793484745907 26-27 24.04722224746897 26.91615225274875 24.78705288128255 24.24957261849973 28-29 24.142543124621337 26.482143894961187 25.84539543607875 23.529917544338726 30-31 24.5675669157865 27.246921138674978 25.144248085703243 23.041263859835283 32-33 23.019067892048263 27.068773327065003 25.749885540229638 24.162273240657093 34-35 22.916286565100847 27.15528639229697 25.711851533333064 24.21657550926912 36-37 24.80858195262067 26.769698492986894 24.694393609307234 23.727325945085205 38-39 24.893555120254373 26.237880028633693 24.864065270866647 24.00449958024529 40-41 23.791759447326125 27.17334959100438 24.73412839430503 24.300762567364465 42-43 24.637416321472376 26.808728980121437 25.055878783139253 23.497975915266938 44-45 24.907500760540454 26.30036490014223 24.800547270575606 23.99158706874171 46-47 24.048354990126214 26.6684952922141 26.07416556407417 23.20898415358552 48-49 24.562078444269325 26.251481456201642 25.75414652610155 23.432293573427483 50-51 23.72165248875281 26.669035196621255 25.597448374696118 24.011863939929825 52-53 23.268477016322848 26.907490845997835 24.74319946541409 25.08083267226523 54-55 23.68816852798899 27.213627205376202 24.3463631134427 24.751841153192117 56-57 24.22226751113062 26.39853891013984 24.420894212077506 24.958299366652035 58-59 24.172552556076802 25.723215471421913 25.35191802271176 24.752313949789528 60-61 24.541854346330677 26.39923205173546 25.05361258253695 24.005301019396914 62-63 23.10965474153069 26.616264120000892 25.580035304324973 24.694045834143445 64-65 22.781102086498723 26.891026242238937 25.425646989676924 24.902224681585412 66-67 23.973973476027176 26.281447377181795 24.888590589645258 24.855988557145775 68-69 24.6395884381969 25.918482129863797 24.557543529818957 24.884385902120346 70-71 23.92435519398828 26.326566175829917 24.806439729191414 24.942638900990385 72-73 24.612053082654313 25.970444229391276 24.93854212616626 24.478960561788146 74-75 24.539400316097087 25.66945490513335 24.833658786253228 24.957485992516336 76-77 23.956507966843006 26.19498883054404 25.63941279284553 24.209090409767427 78-79 24.23002067414369 25.86630720414392 25.666608607410886 24.23706351430151 80-81 23.568161888290746 25.97533980464278 25.60554344384089 24.850954863225585 82-83 23.39424612826842 26.35206091329465 24.91662875076925 25.33706420766768 84-85 23.55226509688201 26.205191129311512 24.604793664654252 25.637750109152226 86-87 23.951681323979564 25.57413934227099 25.022854943058864 25.451324390690583 88-89 23.974680492102443 25.239457755765443 25.650117229651055 25.135744522481062 90-91 24.204964520495334 25.62839553588408 25.328152791191087 24.838487152429497 92-93 22.918092162453167 25.896877800469642 25.75413546121147 25.430894575865725 94-95 22.698009210357014 25.83238825933828 25.828348629069183 25.64125390123553 96-97 23.839062318846 25.57395197151651 25.392863420636314 25.194122289001168 98-99 23.828586379606786 25.10540479928235 25.21619197129401 25.849816849816847 100 23.226982652814403 25.550890353483624 25.782325520281184 25.439801473420793 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 963.0 1 1041.5 2 960.0 3 705.0 4 510.5 5 385.0 6 345.5 7 366.0 8 432.5 9 487.5 10 559.0 11 668.5 12 805.5 13 986.0 14 1201.5 15 1416.5 16 1600.0 17 1777.0 18 2036.0 19 2251.5 20 2344.5 21 2575.5 22 2821.5 23 3057.0 24 3379.0 25 3726.5 26 4164.5 27 4691.0 28 5241.5 29 5713.0 30 6212.0 31 6844.5 32 7623.0 33 8401.5 34 9287.5 35 10571.5 36 12062.0 37 13713.5 38 15342.0 39 16665.5 40 17834.5 41 18768.5 42 19374.5 43 20092.5 44 20646.0 45 21159.5 46 22278.0 47 23856.0 48 25772.0 49 29125.5 50 33049.5 51 32270.5 52 26962.0 53 22586.5 54 20065.0 55 18117.5 56 16524.5 57 15157.0 58 14150.5 59 13516.0 60 12709.5 61 11470.0 62 9943.0 63 8306.5 64 6922.5 65 5799.5 66 4839.5 67 3973.0 68 3134.0 69 2376.5 70 1756.5 71 1372.5 72 1145.5 73 917.0 74 732.5 75 587.0 76 415.0 77 297.5 78 216.5 79 135.5 80 79.5 81 52.0 82 32.0 83 20.0 84 14.5 85 12.5 86 10.0 87 5.0 88 3.0 89 2.0 90 0.5 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.865235415432872 2 24.889367692092506 3 6.481996768078376 4 24.24432256125325 5 13.106096904080971 6 10.040384140371343 7 8.466087142844389 8 9.07065885370942 9 5.69246747990465 10-11 7.101299613419319 12-13 10.593396880570676 14-15 7.7491719316832475 16-17 8.171390053657042 18-19 6.846629546191779 20-21 8.337301315683748 22-23 5.91164890498567 24-25 5.601179085955426 26-27 9.955791716638444 28-29 6.5488820705725495 30-31 4.362275796597256 32-33 9.16150084964422 34-35 7.433047737803662 36-37 7.349273710430536 38-39 6.148463051636346 40-41 8.718450820330752 42-43 6.765385107567042 44-45 6.577377143826464 46-47 6.150695041708454 48-49 4.07858985843231 50-51 6.811810501066891 52-53 4.805995422932359 54-55 5.321064331905852 56-57 5.083878186909825 58-59 3.638441416212581 60-61 4.281477755986941 62-63 3.1869098246250998 64-65 4.808301812673537 66-67 2.784407614954929 68-69 5.4190486960714 70-71 2.6571097811756936 72-73 1.6706445689729572 74-75 0.8622921645244521 76-77 1.1175574291045554 78-79 1.7559809893965592 80-81 2.261452341059987 82-83 0.7439222910336495 84-85 1.1659916136693025 86-87 0.4692387128262054 88-89 0.6457147278608905 90-91 0.23636774863625407 92-93 0.29127470441011477 94-95 0.5457959722995153 96-97 0.533966424917342 98-99 0.4755626846971785 100 0.3440240697809376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 672046.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.74790023339791 #Duplication Level Percentage of deduplicated Percentage of total 1 94.5731154254667 80.14872950839077 2 3.1743630481759606 5.380412058228023 3 0.917657571962381 2.333086570712701 4 0.44136326944113485 1.4961844130113446 5 0.2452479914665452 1.0392126156623998 6 0.1597441270647036 0.8122787606010444 7 0.09342070433627768 0.5542045971577236 8 0.06905733029947338 0.46819709908836615 9 0.05350777783559117 0.4081204635949351 >10 0.25202522494477575 3.638883126594586 >50 0.011662533640457107 0.654720321061486 >100 0.006891296387273882 1.2674794388911144 >500 0.0015902991644450254 1.0644191690221145 >1k 3.5339981432111673E-4 0.734071857983456 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2721 0.40488299908042014 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2181 0.32453135648452636 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 983 0.1462697493921547 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 975 0.14507935468703034 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 938 0.13957377917583025 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 926 0.1377881871181437 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 767 0.11412909235379722 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 718 0.10683792478491055 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 1.4879933814054396E-4 0.0 0.0 1.4879933814054396E-4 0.0 20-21 1.4879933814054396E-4 0.0 0.0 2.975986762810879E-4 0.0 22-23 1.4879933814054396E-4 0.0 0.0 2.975986762810879E-4 0.0 24-25 1.4879933814054396E-4 0.0 0.0 7.439966907027198E-4 0.0 26-27 1.4879933814054396E-4 0.0 0.0 9.671956979135357E-4 0.0 28-29 1.4879933814054396E-4 0.0 0.0 0.002752787755600063 0.0 30-31 1.4879933814054396E-4 0.0 0.0 0.010713552346119166 0.0 32-33 1.4879933814054396E-4 0.0 0.0 0.030652663656952052 0.0 34-35 1.4879933814054396E-4 0.0 0.0 0.06368611672415281 0.0 36-37 1.4879933814054396E-4 0.0 0.0 0.1085491171735268 0.0 38-39 1.4879933814054396E-4 0.0 0.0 0.17461602330792833 0.0 40-41 1.4879933814054396E-4 0.0 0.0 0.2601756427387411 0.0 42-43 1.4879933814054396E-4 0.0 0.0 0.33249212107504544 0.0 44-45 1.4879933814054396E-4 0.0 0.0 0.41626614844817167 0.0 46-47 1.4879933814054396E-4 0.0 0.0 0.5183424944125848 0.0 48-49 1.4879933814054396E-4 0.0 0.0 0.6207164390532791 0.0 50-51 1.4879933814054396E-4 0.0 0.0 0.7255455727732922 0.0 52-53 1.4879933814054396E-4 0.0 0.0 0.8433946485806032 0.0 54-55 1.4879933814054396E-4 0.0 0.0 0.9697996863309952 0.0 56-57 1.4879933814054396E-4 0.0 0.0 1.1116054555789336 0.0 58-59 1.4879933814054396E-4 0.0 0.0 1.271787943087229 0.0 60-61 1.4879933814054396E-4 0.0 0.0 1.4325656279480867 0.0 62-63 1.4879933814054396E-4 0.0 0.0 1.5960961005645447 0.0 64-65 1.4879933814054396E-4 0.0 0.0 1.7897584391544625 0.0 66-67 1.4879933814054396E-4 0.0 0.0 2.0168262291569325 0.0 68-69 1.4879933814054396E-4 0.0 0.0 2.2706779000247006 0.0 70-71 1.4879933814054396E-4 0.0 0.0 2.60138442904206 0.0 72-73 1.4879933814054396E-4 0.0 0.0 2.9210798070370183 0.0 74-75 1.4879933814054396E-4 1.4879933814054396E-4 0.0 3.2482895516080745 0.0 76-77 1.4879933814054396E-4 1.4879933814054396E-4 0.0 3.619692699606872 0.0 78-79 1.4879933814054396E-4 1.4879933814054396E-4 0.0 3.99935421087247 0.0 80-81 1.4879933814054396E-4 1.4879933814054396E-4 0.0 4.43154188850168 0.0 82-83 1.4879933814054396E-4 1.4879933814054396E-4 0.0 4.942072417661887 0.0 84-85 1.4879933814054396E-4 1.4879933814054396E-4 0.0 5.452602946822093 0.0 86-87 1.4879933814054396E-4 1.4879933814054396E-4 0.0 5.977418212443791 1.4879933814054396E-4 88 1.4879933814054396E-4 1.4879933814054396E-4 0.0 6.415334664591412 1.4879933814054396E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACG 115 0.001404262 24.42292 4 GTTCTAA 130 0.0016049561 23.868443 1 ACACTCG 130 2.0201906E-4 23.171534 6 TATACCT 260 3.110472E-10 23.171534 5 GTACAAA 220 2.8930644E-6 22.566528 1 GGTAATA 220 2.898605E-6 22.561556 2 GTATAGT 170 2.9561465E-4 21.902805 1 ATACCTT 230 5.0118615E-8 21.828257 6 TAACGCC 105 0.0081251515 21.39913 4 GTATAGG 215 6.184733E-5 20.204912 1 GTACATA 220 7.380732E-5 19.745712 1 ATATACT 175 7.0407946E-4 19.259216 4 AGTACTC 315 3.4124241E-9 19.125711 5 CATATAC 180 8.515086E-4 18.722372 3 ATGGGTA 380 9.458745E-11 18.496574 5 ATGTAGT 175 0.00897793 17.730843 1 CATGGGT 640 0.0 17.553974 4 TTAATGT 260 2.895743E-5 17.283913 4 GTAATAA 295 4.460324E-6 17.135729 3 CTATACC 230 2.2270532E-4 17.096045 4 >>END_MODULE